KR20210093662A - Cosmetic composition comprising seaweed extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a seaweed extract as an active ingredient. It is possible to prevent and alleviate skin damage due to its antibacterial, antioxidant and anti-inflammatory activity. In addition, it is possible to provide a cosmetic composition capable of exhibiting excellent effects in preventing and alleviating skin aging, reducing skin wrinkles, and alleviating skin inflammation.

Description

Cosmetic composition comprising seaweed extract as an active ingredient {COSMETIC COMPOSITION COMPRISING SEAWEED EXTRACTS}

The present invention relates to a cosmetic composition comprising a seaweed extract as an active ingredient. More particularly, it relates to a cosmetic composition containing a seaweed extract as an active ingredient and exhibiting excellent antibacterial, antioxidant and anti-inflammatory effects.

Recently, research on the development of functional natural materials for the purpose of beauty has been conducted in various ways, and in particular, as the antibacterial, antioxidant, whitening, moisturizing and skin aging inhibitory effects of these various natural substances have been scientifically proven, research on them is attracting attention. there is. The skin is the most external organ of the human body and acts as an essential barrier to protect the body by protecting the body's moisture and blocking intrusion factors from the outside.

Cosmetics generally aim to clean and beautify the skin, and in the process accompanying this purpose, it cleans and moisturizes the skin, blocks UV rays, and removes free radicals and peroxides from the body caused by pollution and stress to reduce skin aging. gives the effect of delaying

The skin has the function of protecting the organs of the body from changes in temperature and humidity and from external environmental stimuli such as UV rays. However, the skin has a lot of opportunities to come in direct contact with stimuli such as ultraviolet rays or various pathogens, and the constant physical and chemical stimuli received from the outside decrease the skin function, and include genetics, inflammation, benign and malignant tumors, hormones, trauma and degenerative diseases. Because it is also affected by the pathological changes of the skin, various causes can cause skin diseases.

Skin aging can be broadly divided into natural aging and extrinsic aging. Extrinsic aging is mainly affected by sunlight, and in particular, ultraviolet rays are well known to cause photo-aging. On the other hand, infrared radiation is known to have a beneficial effect by increasing heat in the body.

One of the causes of skin aging is caused by active oxygen such as superoxide anion radical, hydroxy radical, singlet oxygen, and hydrogen peroxide (H ₂ O _{2 ) derived from oxygen.} It is known that the free-radical reaction destroys major macromolecules of cells including lipids, which are highly reactive and cause abnormal metabolism by reacting with cell components or cell membranes. Exposure to such abnormal metabolism or physical and chemical external stimuli causes an imbalance between oxidation and antioxidant, causing peroxidation or incapacity of the body's defense mechanisms, causing oxidation and denaturation of proteins, and impairing cell functions. Eventually, skin aging is accelerated, which is characterized by loss of elasticity, wrinkles, melasma and freckle production (J. Soc., Cosmet. Sci. Kor., 23, pp75-132, 1997).

In particular, one of the many causes of aging and disease of living things living in the natural world can be found in active oxygen generated during oxidative metabolic processes. Although it is an essential material for non-specifically removing non-magnetic substances, it is known to cause lipid peroxidation by participating in a free-radical reaction in a biofilm mainly rich in unsaturated fatty acids.

Substances with active oxygen suppression and antioxidant effects used as raw materials for general cosmetics, pharmaceuticals, and foods are known, but their use is limited because they are synthetic products that do not secure safety when applied to human skin for a long period of time.

Most of the natural products have a weak inhibitory effect on free radicals, but safety is guaranteed for human skin.

Therefore, there is a hope for a substance that has a strong active oxygen inhibitory effect and an antioxidant effect and also has another effect on human skin.

Inflammatory reaction is a biological defense reaction process for repairing and regenerating damage caused by invasion that causes certain organic changes in cells or tissues of the living body, and local blood vessels, various tissue cells of body fluids, and immune cells act. While the inflammatory response normally induced by external invading bacteria is a defense system to protect the living body, when an abnormally excessive inflammatory response is induced, various diseases appear. These diseases are called inflammatory diseases. The inflammatory disease is a disease in which various inflammatory mediators secreted from target cells activated by external stimuli amplify and sustain inflammation, thereby threatening the life of the human body, and chronic inflammatory diseases in joints such as acute inflammation and rheumatoid arthritis. there is. The key inflammatory mediators that induce these inflammatory diseases are prostaglandins and hydroxy fatty acids, which are produced by cyclooxygenase and the like.

On the other hand, in order to eliminate inflammation, an anti-inflammatory agent that acts to remove an inflammatory source, reduce biological reactions and symptoms. Substances used for anti-inflammatory purposes so far include flufenamic acid, ibuprofen, benzydamine, indomethacin, etc. as a non-steroidal type, and prednisolone as a steroid type. , and dexamethasone. In addition, allantoin, azene, hydrocortisone, etc. are known to be effective in anti-inflammatory, but these substances have limitations in usage due to safety issues for the skin, or have insignificant effects, so that no inflammatory relief effect can be expected. There is this.

KR 10-1752451 B1

An object of the present invention is to provide a cosmetic composition comprising a seaweed extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition comprising a seaweed extract as an active ingredient, and exhibiting antibacterial, antioxidant and anti-inflammatory effects.

Another object of the present invention is to provide a cosmetic composition comprising a seaweed extract as an active ingredient, which can exhibit excellent effects in preventing and improving skin aging, improving skin wrinkles, and improving skin inflammation.

In order to achieve the above object, a cosmetic composition according to an embodiment of the present invention includes a seaweed extract including seaweed extract, kelp extract, laver extract and agar agar extract as an active ingredient.

The composition is to further include an extract of P. oleracea, an extract of water grass, and an extract of Hokdol leaf.

The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof.

The extract is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidium (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) It is fermented with one or more selected from the group consisting of Bacillus acidophilus.

A moisturizing cream according to another embodiment of the present invention is prepared by including the cosmetic composition as an active ingredient.

The whitening cream according to another embodiment of the present invention is prepared by including the cosmetic composition as an active ingredient.

The lotion according to another embodiment of the present invention is prepared by including the cosmetic composition as an active ingredient.

Hereinafter, the present invention will be described in more detail.

As used herein, the term 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the plant extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the plant extract of the present invention.

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

As used herein, the term "prevention" refers to preventing, inhibiting, or delaying skin aging and damage by protecting the skin, and "improvement" refers to the treatment of skin aging and damage or the improvement of symptoms even without complete healing. It means to stop the progression of damage by suppressing tremor or exacerbation and to induce some or all of the symptoms in the direction of healing.

The cosmetic composition according to an embodiment of the present invention includes a seaweed extract including seaweed extract, kelp extract, laver extract and agar agar extract as an active ingredient.

Seaweed (Undaria pinnatifida) is a brown algae in the seaweed family that lives in the sea and is similar to plants, but belongs to a protozoan, not a plant, in classification. It is edible because it contains minerals, vitamins and fiber components, viscous polysaccharides, and iodine. It is a food mainly used in Northeast Asia such as China, Japan, and Korea. Korean records show that it was eaten even in the 12th century, the Goryeo Dynasty, and Chinese records show that Koreans were already eating it in the 8th century. was edible. Dried seaweed contains 12.7% protein, 52% carbohydrate, fat, 1.1%, Ca 1,300mg%, vitamin A 470IU, B1 0.11mg%, B2 0.14mg%, C15mg%, nicotinic acid 10mg%. Silver alginic acid is secreted from the mucous glands and is found in more leaves and stems than roots. Polysaccharides in seaweed are not digested or absorbed by the human body and are excreted as they are, so they are not used as calorie sources. On the other hand, algins are effective in removing harmful metals due to their strong metal absorption, and the fiber containing 5~15% acts as a bowel movement along with polysaccharides. It is a good source of nutrients as it contains iron, zinc, iodine, potassium and calcium as trace minerals. Therefore, seaweed is a food material that nutritionally shows vitamin and mineral source, intestinal function, alkaline food, removal of harmful metals, anti-cholesterol effect, and the like.

Laminaria japonica is a seaweed belonging to the phylum Phaeophyta, Phaeophyceae, Laminariales, and Laminariaceae. The central part of the part forms a thick middle part, and both sides of the middle part have vertical flaws. The stem is usually columnar and upright, and the root shows a dendritic form that branches in the lower part of the stem in a dichotomous manner. The kelp has been widely used for food in Japan and China, including Korea, since ancient times.

Seaweed contains a large amount of protein and dietary fiber useful for health, but has a lipid content of less than 1%. It is a low-calorie food, and contains a large amount of omega-3 fatty acids such as eicosapentaenoic acid (EPA), which are effective in preventing cardiovascular diseases. It is a very good food resource. In addition, seaweed contains a large amount of chlorophyll, carotenoid and phycobilin pigments, which provide color to food, and contain natural antioxidants such as polyphenol compounds, which have been reported to exhibit radical scavenging effects and anti-aging and carcinogenic effects. . However, as shown in most food resources, there are differences in the content of nutrients and functional ingredients as well as the physiological activity depending on the variety and type of laver. In particular, green laver is rich in calcium, which is good for teeth, atopic dermatitis and hypersensitivity dermatitis. It is effective in calming down.

Gelidium amansii is a seaweed of the red algae plant agaricaceae with a body length of 10 to 30 cm and a main axis butterfly of about 1 mm that grows on rocks in the middle and lower parts of the intertidal zone, and is a living organism distributed in Korea, Japan, Indonesia, and the like. Agar agar is well known as a seaweed used as a main raw material for agar production because it contains agar quality, and it is widely used in food production such as jelly and pharmaceutical raw materials in addition to agar production, and as a stabilizer to prevent additives from precipitating during cosmetic production. Seaweed is also used.

The polysaccharides contained in seaweeds as described above are alginic acid and fucoidan, and the alginic acid is a type of dietary fiber that activates intracellular metabolism to make skin and hair healthy, It helps the formation of elastic cells such as collagen and elastin in the skin. It is rich in zinc, which is called a skin mineral, which helps to get rid of skin problems such as acne and pimples.

The fucoidan (Fucoidan) is a substance secreted by seaweed to protect itself from waves and sunlight, and acts as a very excellent natural moisturizer. Through a recent study, fucoidan has been confirmed to have the effect of protecting the skin from UV rays by helping cell activation. .

The composition is to further include an extract of P. oleracea, an extract of water grass, and an extract of Hokdol leaf.

A cosmetic composition is provided by mixing the seaweed extract, kelp extract, laver extract, and agaric agar extract with pink algae extract, water grass extract and humpback leaf extract, and according to the synergistic effect of the mixed use, seaweed extract, kelp extract and laver extract And it is possible to equal or increase the excellent antibacterial activity, antioxidant and anti-inflammatory effects compared to the case of using only the agar agar extract, and further provide a composition with high palatability.

Binghamia californica J. Agardh is a red algae plant that grows in the lower intertidal zone. The plant is membranous, and several flat stems come out of thin pink, string-shaped roots. The stem is bifurcated. Spherical cells form the cortex in 2-3 layers, and in the male tissue, round cells and rod-shaped cells combine to connect both cortical tissues.

Plants of water grass (Gelidiopsis variabilis (J. Agardh) Schmitz) are reddish-brown, cartilaginous, and form small populations by gathering from thin stems that are slightly flat and thorny. Branches are sparse and irregular in length and place of flight. The bead-shaped cells form a cortex in one or two layers, and as they go inward, they grow little by little, and the long, membrane-shaped cells in the middle form a water tissue. It grows on seashells in the subtidal zone.

Lithophyllum okamurai (Lithophyllum okamurai) is a red plant with a thickness of about 0.6mm, envelops the surface of small stones or other objects, and grows with lump-shaped projections. The whole body is covered with calcareous and has a pink or red color. The columnar protrusion corresponds to the branch, and the tip is round and the surface is smooth. In Korea, it is collected from Jeju and the southern coast.

Preferably, the cosmetic composition is based on 100 parts by weight of seaweed extract, 100 parts by weight of kelp, 100 parts by weight of laver extract, and 100 parts by weight of agar agar extract, 30 to 50 parts by weight of Apifolia extract, 30 to 50 parts by weight of water grass extract and lumps Stone leaf extract may be included in an amount of 30 to 50 parts by weight. In the case of the above range, a synergistic effect with a critical significance is expressed as a synergistic effect due to the interaction of each seaweed extract, and when it is outside the above range, the synergistic effect is rapidly reduced or almost absent.

The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof.

The seaweed extract can be eluted with a polar solvent such as an alcohol having 1 to 6 carbon atoms, such as water, ethanol, methanol, etc., or a mixed solvent having a mixing ratio of 1:0.1 to 1:10 of alcohol and water, from a pulverized product of dried seaweed. , More specifically, water may be used as an extraction solvent. At this time, the extraction temperature may be 10 ℃ to 100 ℃, preferably the extract extracted at room temperature.

The extraction solvent may be used in an amount of 2 to 50 times, preferably 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and in particular, from 1 to 24 hours.

It may be a result obtained by concentrating the filtered extract under reduced pressure with a vacuum rotary concentrator, but is not limited thereto, as long as it is a cosmetic composition comprising the seaweed extract of the present invention as an active ingredient, and the extract, the diluted or concentrated solution of the extract, and the extract are dried All of the dried product obtained by the process, a crude product thereof, or a purified product thereof are included.

The extract is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidium (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) It is fermented with one or more selected from the group consisting of Bacillus acidophilus.

More specifically, the cosmetic composition will include a fermented seaweed extract, a fermented kelp extract, a fermented laver extract, a fermented agar agar extract, a fermented pink ragweed extract, a fermented watergrass extract and a fermented Hokdol leaf extract.

When using the fermented seaweed extract, fermented kelp extract, fermented laver extract, fermented agar agar extract, fermented pink chrysanthemum extract, fermented water grass extract and fermented honeysuckle leaf extract, it has low cytotoxicity and can be used stably at a relatively high concentration. And it can increase the preference for falling due to the peculiar odor of seaweed.

In addition, in the case of fermentation, it may be advantageous to obtain an active ingredient exhibiting antioxidant and anti-inflammatory activity.

The microorganisms used for the fermentation include Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus rhamnosus, Lactobacillus Lactobacillus plantarum), Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus Lactobacillus reuteri (Lactobacillus reuteri), Lactobacillus buchneri (Lactobacillus buchneri), Lactobacillus gasseri (Lactobacillus gasseri), Lactobacillus johonsonii (Lactobacillus johonsonii), Lactobacillus kefir, such as Lactobacillus kefir Cocos lactis (Lactococcus lactis), Lactococcus plantarum (Lactococcus plantarum), Lactococcus raffinolactis (Lactococcus raffinolactis), Enterococcus faecalis (Enterococcus faecalis), Enterococcus faecium (Enterococcus faecium) Lactic acid coxae and Bifidobacterium animals such as Streptococcus thermophilus, Leuconostoc lactis, Leuconostoc mesenteroides, etc., Bifidobacterium bifidium (Bifidobacterium bifidum), Bifidobacterium brev (Bifidobact) erium breve), Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium themophilum, Bifidobacterium Bifidobacterium such as Bifidobacterium adolescentis and the like may be included, and more preferably Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum) may be at least one selected from the group consisting of Bifidobacterium, Bifidobacterium breve, Bifidobacterium, and Lactobacillus acidophilus.

Preferably, the cosmetic composition is based on 100 parts by weight of fermented seaweed extract, 100 parts by weight of fermented seaweed extract, 100 parts by weight of fermented laver extract and 100 parts by weight of fermented agar agar extract, 30 to 50 parts by weight of fermented pink ragweed extract, 30 parts by weight of fermented water grass extract. to 50 parts by weight and 30 to 50 parts by weight of the fermented hokdol leaf extract. In the case of the above range, a synergistic effect of critical significance is expressed as a synergistic effect due to the interaction of each extract, and when out of the above range, the synergistic effect is rapidly reduced or almost absent.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, a suspension, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing agent, an oil , powder foundation, emulsion foundation, wax foundation, and spray, etc., but are not limited thereto. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation, oil-in-water (O/W) type or water-in-oil It can be prepared as a (W/O) type formulation or ointment.

In addition, functional substances such as whitening agents, moisturizing agents, anti-inflammatory agents, antibacterial agents, antifungals, vitamins, sunscreen agents, antibiotics, anti-acne agents, perfumes, and dyes may be included, if necessary, in an amount conventionally used in the cosmetic field. It may be included in the cosmetic composition according to the present invention.

A moisturizing cream according to another embodiment of the present invention is prepared by including the cosmetic composition as an active ingredient.

The whitening cream according to another embodiment of the present invention is prepared by including the cosmetic composition as an active ingredient.

The lotion according to another embodiment of the present invention is prepared by including the cosmetic composition as an active ingredient.

The dermatologically acceptable carrier includes purified water, oil, wax, fatty acid, fatty acid alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, etc. However, the present invention is not limited thereto, and the types and concentrations thereof vary, and include parts that those skilled in the art can change within the scope of the present invention.

Cosmetics prepared from the cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Cosmetics with emulsified formulations include nutritional lotions, creams, and essences, and solubilized cosmetics include softened lotions.

The cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically commonly used in the art by containing a dermatologically acceptable medium or base in addition to the extract of the present invention.

Suitable cosmetic formulations include, for example, solutions, gels, solid or kneaded dry products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic It may be provided in the form of a vesicular dispersant, cream, skin, lotion, powder, ointment, spray or in the form of a cone stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essence, pack, foundation, etc., and cleansing, face wash, soap, treatment or liquid cosmetics. etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence, nourishing essence, pack, soap, shampoo, cleansing foam. , cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder or eye shadow.

According to the cosmetic composition comprising the seaweed extract of the present invention as an active ingredient, it is possible to prevent and improve skin aging, skin wrinkles and skin inflammation through antibacterial, antioxidant and anti-inflammatory activities.

1 is an experimental result on the hydrogen peroxide scavenging ability of a cosmetic composition according to an embodiment of the present invention.
2 is an experimental result for DPPH free radical scavenging ability of the cosmetic composition according to an embodiment of the present invention.
3 is a graph showing data comparing the size of edema when the cosmetic composition according to an embodiment of the present invention is treated by concentration.
4 is a graph comparing the amount of prostaglandins when the cosmetic composition according to an embodiment of the present invention is treated by concentration.
5 is a result of evaluating the effect of the cosmetic composition according to an embodiment of the present invention on skin itching symptoms.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example: Preparation of Extract]

One. Preparation of seaweed extract

After washing and drying the seaweed, it was pulverized. The pulverized product was mixed with ethanol and maintained at 98 to 100° C. for 2 hours, cooled and filtered with Whatman filter paper to obtain the filtrate, which was prepared as a seaweed extract (UE).

2. Preparation of other seaweed extracts

In the same manner as the seaweed extract (UE), a kelp extract (KE), laver extract (LE), agaric agaric extract (GE), chrysanthemum extract (BE), water grass extract (VE), and humpback leaf extract (AE) were prepared did.

3. Preparation of fermented seaweed extract

DW 75% (w/w), Ginseng powdered milk 15% (w/w), and seaweed extract (UE) 10% (w/w) are mixed with 'Lactobacillus casei', a microorganism used for fermentation. Fermented seaweed extract (ULc) was prepared by inoculation in a sterile medium and then culturing (25° C., 24 hours).

4. Preparation of other fermented seaweed extracts

In the same manner as the fermented seaweed extract (ULc), fermented kelp extract (KLc), fermented laver extract (LLc), fermented agar agaric extract (GLc), fermented pink ragweed extract (BLc), fermented water grass extract (VLc) and fermented hump Stone leaf extract (ALc) was prepared.

5. Preparation of complex extracts

A complex extract was prepared by mixing the prepared extract in the content range shown in Table 1 below.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 MX10 MX11 MX12 UE 100 100 100 100 100 100 - - - - - - KE 100 100 100 100 100 100 - - - - - - LE 100 100 100 100 100 100 - - - - - - GE 100 100 100 100 100 100 - - - - - - BE - 20 30 40 50 60 - - - - - - VE - 20 30 40 50 60 - - - - - - AE - 20 30 40 50 60 - - - - - - ULc - - - - - - 100 100 100 100 100 100 KLc - - - - - - 100 100 100 100 100 100 LLc - - - - - - 100 100 100 100 100 100 GLc - - - - - - 100 100 100 100 100 100 BLc - - - - - - - 20 30 40 50 60 VLc - - - - - - - 20 30 40 50 60 ALc - - - - - - - 20 30 40 50 60

(Unit: parts by weight)

[Experimental Example 1: Antibacterial activity test]

Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 9027), Candida albicans ( Candida albicans) (ATCC 10231) and Aspergillus brasillensis (ATCC 16404) were evaluated for antibacterial effects using the paper disk diffusion method.

The strain was pre-incubated in Muller-Hinton broth at 35° C. for 24 hours (48 hours for C. albicans). The pre-prepared and sterilized Muller-Hinton solid medium was maintained in a constant temperature water bath at 45° C., and ^{the liquid medium diluted to 1 X 10 6} CFU/ml was inoculated in a Petri dish, followed by control 1 is a conventional synthetic preservative, potassium sorbate, control 2 is methyl-para-benzene, potassium sorbate and methyl-para-benzene are 0.019 to 0.6%, MX1 is diluted to 0.188 to 6% inoculated.

15 ml of the solid medium stored in a 45 ℃ constant temperature water bath was dispensed into each inoculated Petri dish and mixed evenly with the inoculum of the plate. After leaving sufficiently to harden the medium, it was cultured at 35° C. for 24 hours.

The minimum inhibitory concentration (MIC) of MX1 for each strain was determined as the lowest concentration of the sample in which bacterial growth was not observed by visually checking within each plate.

Minimum inhibitory concentration (MIC) Control 1 Control 2 MX1 E. coli > 0.6% 0.15% 3.0% S. aureus > 0.6% 0.30% 3.0% P. aeruginosa > 0.6% 0.15% 1.5% C. albicans > 0.6% 0.30% 3.0% A. brasillensis > 0.6% 0.30% 3.0%

It was confirmed that MX1 of the present invention exhibits significantly excellent antibacterial activity against a wide range of strains.

[Experimental Example 2: Anti-aging effect test by antioxidant]

cell

HaCa T cells were maintained in 10% FCS-DMEM medium supplemented with streptomycin and penicillin.

Hydrogen peroxide scavenging ability

Hydrogen peroxide solution (40 mM) was prepared in phosphate buffer (pH 7.4). MX1 mixed with seaweed extract was added to hydrogen peroxide solution (0.6 mL, 40 mM). The absorbance of hydrogen peroxide at 230 nm for a blank solution containing a phosphate buffer without hydrogen peroxide was measured after 10 minutes. The percentage of hydrogen peroxide scavenging capacity of both sample and standard compounds was calculated:

[Equation 1]

[H2O2] = [(AC - AS)/AC] × 100

where AC is the absorbance of the control and AS is the absorbance in the presence of a sample or standard sample.

As shown in FIG. 1 , it was confirmed that the hydrogen peroxide scavenging ability was improved as the content of MX1 was increased.

Evaluation of DPPH free radical scavenging ability

10 mg DPPH was dissolved in 100 ml methanol (MeOH) to 100 μg/ml. A stock solution of the extract was prepared by dissolving 25 mg in 50 ml of MeOH.

1 ml DPPH was added to each solution and the solution was kept dark and allowed to stand for exactly 30 minutes. The UV absorption of each solution was recorded at 517 nm. The reaction was allowed to proceed at 37 °C for 30 min and absorbance was monitored with a microplate reader at 517 nm. The % radical scavenging activity (% RSA) was determined compared to the DMSO containing control. The scavenging of free radicals by DPPH as a radical scavenging activity (%) was calculated as follows:

[Equation 2]

% RSA = (Control Absorbance - Absorbance)X100/Control Absorbance

The result is shown in FIG. 2 . According to FIG. 2, when 25 mg/ml, 50 mg/ml and 100 mg/ml of MX1 were used, that is, it was confirmed that the DPPH free radical scavenging ability was improved as the content increased. According to the experimental results, in the case of MX1 mixed with seaweed extract, DPPH radical scavenging ability is excellent, so it will be said that it is excellent in anti-aging effect by antioxidant.

[Experimental Example 3: Anti-inflammatory effect test]

The anti-inflammatory effect of MX1 mixed with seaweed extract was evaluated to confirm the effect of inhibiting and improving skin inflammation.

In order to induce atopic dermatitis as an experimental method, 200 μl/mouse/day of a 1% DNCB (Sigma-Aldrich, St.Loulse, MO, USA) solution dissolved in a mixture of acetone and olive oil (3:1) was administered once daily for 7 days. It was applied to the entire dorsal skin of hairless mice for sensitization, and boosted by applying 0.5% DNCB solution 3 times a week for 4 weeks. To the skin with atopic dermatitis, the concentration of MX1 mixed with seaweed extract was increased to 10% and 20%, and diluted samples were respectively applied to observe the size of edema.

In addition, prostaglandins are characterized by their role in anti-acid maintenance, vasodilation, smooth muscle stimulation, and mediator of inflammation, and to induce inflammation and pain while protecting cells from apoptosis. Accordingly, the amount of protaglandin was determined by observing that serum was extracted every 7 days from mice immunized with KLH.

3 and 4, it was found that the concentration of MX1 mucus mixed with seaweed extract affects the anti-inflammatory effect.

[Experimental Example 4: Antibacterial, antioxidant and anti-inflammatory effect test of complex extract]

Antibacterial activity test

The antimicrobial activity test was carried out by the same method as the antimicrobial activity test of MX1 in Example 1.

Evaluation of DPPH free radical scavenging ability

The experiment was carried out by the same method as the previously evaluated DPPH free radical scavenging ability.

After the same treatment with 100 mg / ml, DPPH free radical scavenging ability was evaluated.

Anti-inflammatory evaluation

The experiment was carried out in the same manner as in the evaluation of inflammation of MX1 mixed with seaweed extract.

The size of the edema was observed by increasing the concentration of the extract to 20%.

For relative comparison of the results, the effect of MX1 mixed with seaweed extract was set to index 5, and the complex extracts MX2 to MX12 were evaluated as indices, and are shown in Table 3 below. The higher the index, the better the effect.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 MX10 MX11 MX12 antibacterial activity 5 6 7 8 9 7 8 9 10 10 9 8 DPPH free radical scavenging ability 5 5 6 7 8 6 7 7 8 9 8 7 size of edema 5 6 6 7 7 6 7 8 9 10 8 8

(Unit: Index)

As shown in Table 3 above, it was confirmed that MX1 and complex extracts MX2 to MX12 mixed with seaweed extract exhibited antibacterial activity equal to or superior to MX1 and DPPH free radical scavenging activity, and complex ferment extracts (MX6 to MX12) showed low It showed excellent antibacterial, antioxidant and anti-inflammatory effects as stability was increased due to cytotoxicity.

Based on the experimental results, it will be said that it is excellent in antibacterial, anti-oxidation and anti-inflammatory action, and skin inflammation improvement.

In particular, it can be seen that in the case of the complex extract in the range of the fermented complex extract MX9 to MX11, the synergistic effect more than a simple combination appears due to the synergistic action by mixing each extract.

[Experimental Example 5: Skin wrinkle improvement and skin moisturizing effect]

Skin wrinkle improvement effect

Among healthy women over the age of 40, those with wrinkles around the eyes were selected from the questionnaire and physical examination. The wrinkle area around the eyes was determined as the test site.

The cosmetic compositions MX1 to MX12 were prepared as moisturizing creams, and after they were randomly assigned to the recruited experimenters, an appropriate amount was applied to the test site, the wrinkle area, twice a day for 6 weeks.

Four experimenters were assigned to each experimental group, and the experiment was conducted for a total of 28 people. A replica of the facial wrinkle test area was made and a Visiometer, SkinVisiometer SV 600, Courage & Khazaka, Germany ) was used to perform image analysis on the skin wrinkle replica. For wrinkle analysis, the change in the depth of skin wrinkles was analyzed by determining the depth of wrinkles by the contrast of shadows generated by irradiating light at a certain angle to the wrinkles of the replica plate.

As the depth of the wrinkles decreases, it means that the skin wrinkles are improved.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 MX10 MX11 MX12 0 weeks 0.25±0.12 0.26±0.12 0.26±0.10 0.26±0.14 0.28±0.12 0.27±0.11 0.27±0.11 0.27±0.54 0.28±0.21 0.28±0.22 0.28±0.14 0.27±0.22 6 weeks 0.25±0.11 0.25±0.11 0.24±0.12 0.24±0.10 0.25±0.10 0.26±0.14 0.23±0.08 0.22±0.69 0.23±0.12 0.23±0.04 0.24±0.22 0.25±0.10

(Unit mm, means mean±standard deviation)

According to the experimental results, it can be confirmed that MX1 mixed with seaweed extract and complex extracts (MX2 to MX12) exhibit the same or superior anti-wrinkle effect as MX1.

skin moisturizing effect

The skin moisturizing effect of the prepared moisturizing cream was evaluated for 10 women aged 20 to 25 years.

The test subjects used the moisturizing cream after washing their face twice every morning (between 7:00 and 9:00) and in the evening (between 7:00 and 9:00) every 12 hours for 4 weeks. At the 4th week, the moisturizing effect of the skin was evaluated by measuring the skin moisture evaporation rate.

The skin moisture evaporation rate was measured with Tewamter TM300 and was expressed as an average value of 10 subjects, and the results are shown in Table 5.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 MX10 MX11 MX12 Water evaporation rate (g/h/m ² ) 20.22 20.21 19.77 18.24 18.12 18.34 18.02 17.58 17.16 16.74 17.28 18.08 Decrease rate of water evaporation compared to before first use (%) 28.50 30.19 35.42 35.59 39.16 35.24 37.22 39.54 39.55 38.42 37.18 37.20

As shown in Table 5, when the cosmetic compositions MX1 to MX12 prepared in Preparation Example of the present invention were prepared as a moisturizing cream and continuously used, it was confirmed that the skin moisture evaporation rate was gradually reduced.

In particular, in the case of the cosmetic compositions MX9 to MX11 using the complex fermented extract, even after 12 hours have elapsed after application, it was confirmed that the effect was significantly reduced compared to the moisture evaporation rate of the skin before use.

sensory test

In addition to the skin wrinkle improvement and skin moisturizing effect test, a moisturizing cream was applied to the wrinkles around the eyes, and a sensory test for spreadability and absorbency was performed.

Evaluation was requested on a scale of 1 to 10, and a higher score would mean better evaluation.

MX1 MX2 MX3 MX4 MX5 MX6 MX7 MX8 MX9 MX10 MX11 MX12 spreadability One 2 4 4 4 3 4 5 7 8 7 5 absorbency One 2 3 4 4 3 5 6 7 8 7 6

According to the sensory evaluation, it was confirmed that MX1 mixed with seaweed extract and MX2 to MX12 complex extracts exhibited superior applicability and absorbency than MX1 mixed with seaweed extract.

In addition, in the case of MX1 in which the seaweed extract is mixed and the complex fermented extracts MX7 to MX12, the characteristic odor of seaweed is neutralized and the preference can be increased.

Therefore, in the case of a moisturizing cream containing the complex fermented extracts MX8 to MX11, it will be confirmed that it can be provided as a functional moisturizing cream with high preference.

Formulation Example 1. Moisturizing cream

A moisturizing cream containing the MX1 to MX12 was prepared in a conventional manner according to the following composition ingredients and composition ratios.

MX1 3.0 wt%

8.0 wt% glycerin

Butylene glycol 5.0 wt%

Glyceryl Stearate / PEG-100 Stearate 1.0 wt%

Polysorbate 60 0.5 wt%

0.5 wt% sorbitan stearate

Phytosqualane 5.0 wt%

jojoba oil 3.0 wt%

15.0 wt% caprylic/capric triglycerides

Aloe Butter 2.0 wt%

beeswax 1.0 wt%

0.5% by weight of stearyl alcohol

0.5% by weight of beheryl alcohol

0.5% by weight of stearic acid

0.2 wt% of carboxyl vinyl polymer

0.1% by weight of xanthan gum

Arginine 0.5 wt%

Dimethicone 0.5 wt%

Beta glucan 0.2 wt%

0.5% by weight of preservative

Purified water remaining

Although the preferred embodiment of the present invention has been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

Contains seaweed extract, kelp extract, seaweed extract and agar agar extract as active ingredients.
A cosmetic composition comprising a seaweed extract as an active ingredient. The method of claim 1,
The seaweed extract further comprises an extract of Apifolium oleracea, an extract of water grass, and an extract of Hokdol leaf.
A cosmetic composition comprising a seaweed extract as an active ingredient. The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof.
A cosmetic composition comprising a seaweed extract as an active ingredient. The method of claim 1,
The extract is Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), Bifidobacterium bifidium (Bifidobacterium bifidum) Bifidobacterium, Bifidobacterium breve (Bifidobacterium breve) Bifidobacterium, and Lactobacillus Bacillus acidophilus (Lactobacillus acidophilus) that is fermented with one or more selected from the group consisting of
A cosmetic composition comprising a seaweed extract as an active ingredient. A moisturizing cream comprising the cosmetic composition according to any one of claims 1 to 4. A whitening cream comprising the cosmetic composition according to any one of claims 1 to 4. A lotion comprising the cosmetic composition according to any one of claims 1 to 4.

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