WO2021096186A1 - Cosmetic composition for skin improvement comprising taraxacum officinale leaf extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin improvement comprising a Taraxacum officinale leaf extract. The cosmetic composition of the present invention activates the proliferation of lactic acid bacteria in the skin to promote skin beneficial bacteria, and does not kill skin harmful bacteria but inhibits same, thereby exhibiting an effect of regulating the growth of skin beneficial bacteria and skin harmful bacteria.

Description

Cosmetic composition for skin improvement comprising extract of western dandelion leaf

The present invention relates to a cosmetic composition for improving skin comprising an extract of Western dandelion leaves (Taraxacum officinale).

Microbiome is a compound word of microbiota, which represents a microbial community, and genome, which represents a genome. Microbiota is a term that focuses on the microbial strain itself compared to the microbiome. It refers to a microorganism that lives in the human body and has a symbiotic relationship. It refers to research and technology based on genomics of the interaction between the two. However, recently, microbiota, microflora, and microbiome, which mean microorganisms, are commonly used. The term microbiome is better known as the intestinal microbiome, and inhibits harmful bacteria by ingesting lactic acid bacteria to expand the power of the intestinal lactic acid bacteria, and both lactic acid bacteria and harmful bacteria correspond to microbiome.

One of the reasons microbiome is receiving great attention in recent years is that its applicability to human health and disease issues has been greatly expanded. Since the association between the body's microbes and metabolic-related diseases such as obesity and diabetes has been revealed, the relationship between cancer, infection, immunity, and mental illness has been revealed, and microbiome-based therapeutics are emerging as one of the innovative treatment technologies. Maintaining the balance of the microbiome is well known to affect the functioning of the human body, and interest in microbiome research is increasing as research that the microbiome in the body is associated with obesity is revealed.

In the currently forming human microbiome market, the functional food or Wennis product market, centered on probiotics and prebiotics, accounts for 83% of the total market. With the rapid growth of the health functional probiotics market, the demand for research and development in the fields of cosmetics, medicine, and pharmaceuticals as well as food is increasing rapidly.

Recently, research on applying microbiome to the skin is also actively being conducted. When Staphylococcus aureus and Propionibacterium acnes, known to be harmful to the skin, increase, and Staphylococcus epidermidis, known to be beneficial to the skin, decreases, The increase in acne has already been known by research. In order to prevent or recover from this, many studies are also being conducted in the cosmetic field, but most of them focus only on the antiseptic effect on harmful bacteria.

(Patent Document 0001) Korean Patent Registration No. 10-1376202 (2014.03.13) discloses a pharmaceutical composition for preventing or treating skin diseases containing a mixture extract of dandelion and barley.

(Patent Document 0002) Korean Patent Registration No. 10-1425757 (2014.07.25) discloses a skin massage composition for improving acne and a mask pack including the same.

The present invention is to develop and provide a novel cosmetic composition having skin microbiome effect using a prebiotic material that increases the growth of lactic acid bacteria in the skin.

The present invention provides a cosmetic composition for improving skin comprising an extract of Western dandelion leaves (Taraxacum officinale).

In the cosmetic composition for improving skin of the present invention, the skin improvement is preferably an improvement in acne skin and an improvement in skin oil/moisture balance.

The cosmetic composition for improving skin of the present invention preferably promotes the growth of beneficial skin bacteria and inhibits the growth of harmful skin bacteria.

In the cosmetic composition for improving skin of the present invention, the beneficial bacteria are preferably Lactobacillus plantarum and Staphylococcus epidermidis.

In the cosmetic composition for improving skin of the present invention, the harmful bacteria are preferably Staphylococcus aureus and Propionibacterium acnes.

The cosmetic composition of the present invention promotes skin beneficial bacteria by activating the proliferation of lactic acid bacteria in the skin, does not kill and inhibits skin harmful bacteria, thereby exerting the effect of controlling the growth of beneficial skin bacteria and harmful bacteria.

1 is a result of evaluating the efficacy of controlling skin lactic acid bacteria (L. plantarum), beneficial bacteria (S. epidermidis) and harmful bacteria (S. aureus and P. acne) of Example 1.

2 is a result of confirming changes in skin oil/moisture and pH of a subject during the treatment of Example 1. FIG.

3 is a result of confirming changes in bacterial porphyrin during treatment in Example 1.

4 is a result of confirming the change in skin microbial distribution during treatment in Example 1 (Bad: Staphylococcus aureus, Pseudomonas aeruginosa, Corynebacterium jeikeium, Corynebacterium diphtheriae, Streptococcus pyogenes, Streptococcus cristatus, Acinetobacter junii, Finephogoldia magna, Stenotrobacterium, Stenotrobacterium P.acne: Propionibacterium acnes, Normal: P.Koreensis, Propionibacterium qranulosum, Staphylococcus epidermidis, Streptococcus salivarius, Neisseria subflava, Staphylococcus warneri, Pseudomonas tolaaii, Acidovorax temperaceticans, Dietozia Lactobacillus, Bradyrhis , Lactobacillus rhamnosus HK-9, Bacillus licheniformis, Bacillus subtilis).

The present invention provides a cosmetic composition for improving skin comprising an extract of Western dandelion leaves (Taraxacum officinale).

Probiotics control the composition of the microbiome, which also applies to the skin's microbiome. A representative example of probiotics is lactic acid bacteria, and the lactic acid bacteria play a role of suppressing harmful bacteria by using a substance called bacteriocin. Existing studies have confirmed that certain lactic acid bacteria and metabolites are effective against skin diseases such as atopy.

However, the use of probiotics (live bacteria) as a cosmetic material exceeds the microbial limit, so it is difficult to commercialize it. Therefore, it was attempted to develop a material having skin microbiome efficacy by using a prebiotic material that increases the growth of lactic acid bacteria in the skin.

Accordingly, the present invention provides a cosmetic composition for improving skin comprising an extract of Western dandelion leaves (Taraxacum officinale).

In the case of a material containing sugars, which is well known as a nutrient source of probiotics, there is a concern of formulation instability when applied to cosmetics in the future. Accordingly, the applicant of the present invention confirmed that the probiotic efficacy of Western dandelion leaves (Taraxacum officinale) was very excellent, and was selected as the material of the present invention.

In the cosmetic composition for improving skin of the present invention, the skin improvement is preferably an improvement in acne skin and an improvement in skin oil/moisture balance. It was confirmed through the following experiment that the extract of Western dandelion leaf of the present invention effectively improves the oil/moisture balance.

The cosmetic composition for improving skin of the present invention preferably promotes the growth of beneficial skin bacteria and inhibits the growth of harmful skin bacteria. At this time, the beneficial bacteria are preferably Lactobacillus plantarum and Staphylococcus epidermidis, and the harmful bacteria are preferably Staphylococcus aureus and Profi. It is better to be Propionibacterium acnes.

In the following experiment, the extract of Western Dandelion leaves of the present invention increases the growth of Lactobacillus plantarum and Staphylococcus epidermidis, which are beneficial bacteria for skin, while increasing the growth of Staphylococcus epidermidis, Staphylococcus aureus, which is harmful to the skin ( Staphylococcus aureus) and Propionibacterium acnes. In particular, as Staphylococcus epidermidis, which is a skin beneficial bacteria, is killed by cosmetic preservatives, the microbiome balance is destroyed.The western dandelion leaf extract of the present invention treats the concentration of the preservative formulation applied to the finished product. As a result of performing the experiment, it was confirmed that the growth of Staphylococcus epidermidis is increased (not shown), and the above problem can be solved.

Meanwhile, in the present invention, the extract is preferably contained in an amount of 0.00001 to 30.0% by weight based on the total weight of the cosmetic composition. More preferably, it is preferably contained in an amount of 0.01 to 10.0% by weight based on the total weight of the cosmetic composition. When the content of the mixed extract is less than 0.00001% by weight, skin improvement effects including improvement of acne skin and skin oil/moisture balance improvement do not appear, and when it exceeds 30.0% by weight, a distinct effect due to the increase in content does not increase.

On the other hand, the ingredients included in the cosmetic composition of the present invention are active ingredients

In addition to the mixed extract of the present invention, components commonly used in cosmetic compositions may be included, and for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers are included.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray may be formulated, but is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. Can be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the cleansing formulation without surfactant is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the configuration of the present invention will be described in detail through the following examples and experimental examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes modifications of equivalent technical ideas.

(Experimental Example 1: Isolation and Identification of Lactobacillus Skin)

In 44 male and female subjects, microbes on the skin surface of the face, cheeks, and palms were swapped. After the swab was put into the tube and concentrated culture was performed, the culture solution was cultured on BHI agar medium to obtain colonies. After culturing a plurality of single colonies in each BHI medium, a sequencing analysis was requested to Macrogen Co., Ltd. to determine the lactic acid bacteria for the cultured strain.

For the identification of the isolated strain, the nucleotide sequence was analyzed using the 16S rRNA method, and it showed 99% homology with the standard strain, so it was deposited with the Biological Resource Center to receive the accession number SD1323. The isolated lactic acid bacteria was named Lactobacillus plantarum RD-02, and was used in the following experiment to confirm the efficacy of the extract of the present invention on skin lactic acid bacteria.

(Example 1: Preparation of the present invention Western dandelion leaf extract)

The dried western dandelion leaves were pulverized finely using a grinder and then powdered. After mixing 50g of western dandelion leaf powder and 1L of purified water, an extract solution was prepared by stirring at 20~25℃ for 1 hour using a stirrer. After the extraction solution was first filtered using a nonwoven filter paper, the first filtrate was changed to No. It was filtered under reduced pressure using 5C filter paper.

(Experimental Example 2: Evaluation of skin microbial control efficacy)

In order to evaluate the skin microbial control efficacy, the skin lactic acid bacteria'Lactobacillus plantarum RD-02 (Lactobacillus plantarum RD-02) and Staphylococcus epidermidis','Lactobacillus plantarum RD02 (Lactobacillus) plantarum RD-02) and Staphylococcus aureus', Lactobacillus plantarum RD-02 (Lactobacillus plantarum RD-02) and Propionibacterium acnes' at the same concentration. Then, they were cultured together with each other, and treated with the extract of Western dandelion leaves and cultured together. Thereafter, the culture solution was diluted and spread on an agar medium, and then the colonies of each cultured microorganism were counted to confirm the change in the number of microorganisms.

As a result of the experiment, by activating the proliferation of Lactobacillus plantarum RD-02 (accession number SD1323), a skin lactic acid bacteria, the extract of Western Dandelion leaves significantly increased the growth of Staphylococcus epidermidis, a beneficial bacteria of the skin, and harmful bacteria for the skin. In Staphylococcus aureus (Staphylococcus aureus) and Propionibacterium acne (Propionibacterium acnes) was confirmed to significantly reduce the growth (Fig. 1). 1 is a result of evaluating the efficacy of controlling skin lactic acid bacteria (L. plantarum), beneficial bacteria (S. epidermidis) and harmful bacteria (S. aureus and P. acne) of Example 1.

(Experimental Example 3: Skin safety evaluation)

In order to confirm the safety of the skin, a human patch experiment was performed. The diluted sample was treated on the inner part of the arm of 20 healthy adult women using the Finn chamber kit and fixed to the skin. The criteria for the judgment were based on the standards of the International Contact Dermatitis Research Group. As a result of the experiment, it was confirmed that the extract of Western dandelion of the present invention was not irritated (Table 1).

sample	Index under test	Judgment result				Irritation
		++	+	±	-
Example 1	20	0	0	0	20	0
Control	20	0	0	0	20	0
(Criteria) -: No erythema or unusual phenomena ±: slightly redder than the surrounding area +: Remarkably redder than surroundings ++: Reddens and swells more severely than the surroundings (Irritation degree calculation formula) Stimulus = [{(±) number×1}+{(+) number×2}+{(++) number×3}/number of subjects

(Experimental Example 4: Evaluation of skin improvement efficacy)

When applied to the actual skin, after applying the diluted sample of Example 1 (0.05% dry weight concentration) to the skin in order to confirm the improvement effect, changes in the oil/moisture, pH, distribution of bacterial porphyrin and microorganisms of the skin Was confirmed.

The oil/moisture and pH measurements were performed using a Soft Plus instrument manufactured by Callegari s.r.l, and the OBSERV instrument manufactured by Sylton Diagnostic System was used to measure bacterial porphyrin. Changes in skin microbes were analyzed by Seva Biotech.

As a result of the experiment, according to the treatment of Example 1, the oil/moisture level of the skin was improved to the level of normal skin. When viewed as an average for all subjects, moisture was improved by 10% and oil content was improved by 20% (FIG. 2). 2 is a result of confirming changes in skin oil/moisture and pH of a subject during the treatment of Example 1. FIG.

Bacterial porphyrin was reduced by about 25% when treated with Example 1. This value is a value derived from the bacterial porphyrin at the tip of the nose, and it was confirmed that the dot representing the bacterial porphyrin was decreased even at a region other than the tip of the nose (FIG. 3). 3 is a result of confirming changes in bacterial porphyrin during treatment in Example 1.

On the other hand, as a result of confirming the change in the distribution of microorganisms, when treated in Example 1, the distribution of the bad type microbial community and the P.acne community decreased, and the normal type and good type microbial community distribution increased (Fig. 4). 4 is a result of confirming the change in skin microbial distribution during treatment in Example 1 (Bad: Staphylococcus aureus, Pseudomonas aeruginosa, Corynebacterium jeikeium, Corynebacterium diphtheriae, Streptococcus pyogenes, Streptococcus cristatus, Acinetobacter junii, Finephogoldia magna, Stenotrobacterium, Stenotrobacterium P.acne: Propionibacterium acnes, Normal: P.Koreensis, Propionibacterium qranulosum, Staphylococcus epidermidis, Streptococcus salivarius, Neisseria subflava, Staphylococcus warneri, Pseudomonas tolaaii, Acidovorax temperaceticans, Dietzobium, Hs. , Lactobacillus rhamnosus HK-9, Bacillus licheniformis, Bacillus subtilis).

The present invention relates to a cosmetic composition for improving skin comprising an extract of Western dandelion leaves (Taraxacum officinale).

Claims (5)

1. Western dandelion leaf (Taraxacum officinale) cosmetic composition for skin improvement comprising an extract.
2. The method of claim 1,

   The skin improvement,

   A cosmetic composition for improving skin, characterized in that it improves acne skin and improves skin oil/moisture balance.
3. The method of claim 1,

   The cosmetic composition,

   A cosmetic composition for improving skin, characterized in that it promotes the growth of beneficial skin bacteria and inhibits the growth of harmful skin bacteria.
4. The method of claim 3,

   The beneficial bacteria are,

   Cosmetic composition for skin improvement, characterized in that Lactobacillus plantarum (Lactobacillus plantarum) and Staphylococcus epidermidis (Staphylococcus epidermidis).
5. The method of claim 3,

   The harmful bacteria,

   Staphylococcus aureus (Staphylococcus aureus) and Propionibacterium acne (Propionibacterium acnes), characterized in that the cosmetic composition for skin improvement.

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