JP2002302416A - Fat synthesis-accelerating agent and cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a fat synthesis-accelerating agent and to prepare a cosmetic each being incorporated with a useful plant extract liquid having the fat synthesis-accelerating effect as recognized in each of various extracts from each of various plants as an active ingredient. SOLUTION: The excellent fat synthesis-accelerating agent and the cosmetic based on the fat synthesis-accelerating effect of each of the various extracts from each of the various plants are provided. Also, since the above fat synthesis- accelerating agent is a natural product contained in each of the various extracts from each of the various plants, it is safe and is also stable to heat, has less adverse effect and can open up uses widely by exhibiting a marked effect not only in the cosmetic field such as the above cosmetic, but also in each of various technological fields such as a medicine and food.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fat synthesis promoting agent and a cosmetic, which have an effect of promoting fat synthesis using various extracts of various plants as active ingredients. In particular, the cosmetics according to the present invention are feminine "fluffy"
The purpose of the present invention is mainly to provide a cosmetic for breast augmentation of breasts, but the field of application of the present invention is not limited to the cosmetics field of the cosmetics, such as pharmaceuticals and foods. It can be widely applied to various technical fields.

[0002]

2. Description of the Related Art In recent years, health consciousness centering on diet has been increasing in various fields, and beautiful and thinness has become an eternal desire especially for women. I have. Not surprisingly, interest in this aspect has grown in the cosmetics field, and a variety of slimming products have hit the shelves.

[0003] On the other hand, from the point of "want to maintain femininity", on the other hand, the desire to "puffy" the underside of the cheekbones, under the eyes, the depression of the face, etc., and to augment the breast, which is a symbol of female beauty There is also. Behind this is the desire of women to be beautifully balanced.

[0004] In general, as a method of making "fluffy",
There are ways to improve the metabolism of collagen (elastin) and collagen (elastin) in the dermal extracellular matrix, but they are not effective, but rather increase and accumulate adipose tissue, which accounts for most of the "plumping" factors. Think effective and direct.

[0005] In addition, fat tissue is increased and accumulated by the fat cells constituting the tissue, which synthesize fat. However, the fat cells under the cheekbones, under the eyes, in the hollows of the face and the breast, are compared with the lower legs. It has a low fat synthesis effect and a high lipolysis effect, and the formation of a feminine "fluffy" contour, breast augmentation promotes fat synthesis in these adipocytes and increases and accumulates adipose tissue. It is necessary to encourage. However, fat synthesis promoters and cosmetics that solve these problems have not been found so far.

From the above, the feminine "fluffy"
There has been a demand for a fat synthesis promoter and a cosmetic that promote the formation of a contour, a fat synthesis in fat cells such as under the cheekbone and under the eyes, a depression in the face and a breast, and promote an increase and accumulation of adipose tissue.

[0007]

Means for Solving the Problems In view of the above problems, the present inventors have conducted intensive studies, and as a result, have found that extracts of various plants have an effect of promoting fat synthesis.

That is, the present invention relates to Kawakawa (Pipe
r methodicum Forst. ), Versicolora (Verbena officinalis)
L. ), Kohakobe (Stellaria media)
(L.) Vill. ), Dandelion (Tarax)
acum officinale Weber, Tar
axacum sec. Ruderalia spe
ces), Ficus pumila (Filipend)
ura ulmaria (L.) Maxim. ), Sunflower (Helianthus annuus L.),
Majorana (Origanum majorana)
L. ), Purple clover (Trifolimpra)
tense L. ), Rattanii (Krameriat)
riandra Ruiz et Par. ), Larea (Larrea tridentata, Larrea)
one or more plant extracts selected from M. mexicana), which are safe without side effects and have a stable effect of promoting fat synthesis, and fats containing these active ingredients. May provide synthesis accelerators and cosmetics.

[0009]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail. "Kawakawa (Piper methisti)" used in the present invention
cum Forst. ")" Is a shrub of the family Pepperaceae from Polynesia, and all plants commonly referred to as Kawakawa can be used.

[0010] As used in the present invention,
ena officinalis L. ) "Is a medicinal herb (Herbs) of the family Verbenaceae.
Thus, all the plants commonly referred to as black pine can be used.

In the present invention, "Kohakobe (Stell)" is used.
aria media (L.) Vill. ) "Is a herb of Caryophyllaceae (H)
erbs), all plants commonly called chickweed can be used.

The dandelion (T) used in the present invention
araxacum officinale web
r, Taraxacumsect. Ruderalia
Species) ”is a kind of Asteraceae (Rosaceae)
Herbs, and all plants commonly called dandelions can be used.

[0013] As used in the present invention, “Filipendula ulmaria (L.) M
axim. ")" Is a medicinal herb (Herbs) of the family Rosaceae, and all plants commonly referred to as eucalyptus can be used.

[0014] The "sunflower" (Helia) used in the present invention.
nthus annuus L .; ) "Means Asteraceae (Co
All of the plants commonly referred to as sunflowers can be used.

[0015] The "Majorana" (Origa) used in the present invention is used.
num majorana L .; ) "Means the Labiatae (La)
All of the plants commonly referred to as Majorana can be used as the herb (Biatae).

The "red clover" (T) used in the present invention
rifolium platenense L. ) "Is a herb of Leguminosae (Herbs)
Thus, all plants generally called clovergrass can be used.

In the present invention, "Lattany (Krame)" is used.
ria triandra Ruiz et Pa
r. ) "Means legumes from South America (Leguminosae)
In the small shrub, all plants commonly called rattany can be used.

As used in the present invention, "Larrea"
tridentata, Larrea mexica
na) "is an evergreen shrub of Zygophyllaceae from northern Mexico (such as Arizona),
All plants commonly called larea can be used.

In addition, various extracts of each plant are obtained by drying some or all of the flowers, whole plants or their leaves, branches, bark, roots, etc. Or, after pulverization without drying, water and / or a lower alcohol or an aqueous solution of a lower alcohol such as methanol, ethanol, or propanol, or a polyhydric alcohol or an aqueous solution of a polyhydric alcohol such as propylene glycol, 1.3-butylene glycol, or glycerin are used alone. And / or two or more solvents can be used in any combination.

The extract of each plant with lower alcohol is as follows:
An extract can be obtained using a Soxhlet extractor. For various plant extracts, lower alcohols, various organic solvents or organic solvent aqueous extracts are concentrated by distillation by removing the lower alcohols and organic solvents, respectively, and for water extracts, the extracts are concentrated under reduced pressure. After that, each extract can be obtained by freeze-drying. In addition,
Specific examples of the preparation of various plant extracts will be described in detail in Examples.

The amount (content) of various active ingredients (various extracts of each plant) in the fat synthesis promoter according to the present invention.
Is not particularly limited because it can be varied according to the kind of the active ingredient and the combination thereof, the purpose of use, the mode, the use form, the number of uses, and the like. In principle, it is sufficient that an effective amount is present, but generally 0.0001 to 100 for each composition of the fat synthesis promoter.
%, Preferably 1 to 10% by weight is available. Furthermore, although the active ingredient (various extracts of each plant) according to the present invention can exert an effect even by one kind,
By using two or more kinds of active ingredients in appropriate combination, an excellent synergistic effect can be expected.

The measurement of the fat synthesis-promoting effect of various extracts of each plant according to the present invention was carried out by the following method.

The effect of promoting fat synthesis was measured using an adipocyte precursor cell line (mouse 3T3-L1: manufactured by Dainippon Pharmaceutical). That is, triglycerides (neutral fats) in adipocytes were extracted with isopropanol, quantified using acetylacetone, and searched for the presence or absence of an active ingredient. The specific measurement method and the method of calculating the fat synthesis promotion rate (%) will be described in detail in the Examples section.

The increase and accumulation of adipose tissue is achieved by the synthesis of fat by the fat cells constituting the tissue. Featuring a low synthetic effect and high lipolytic action, the formation of a feminine "fluffy" contour, breast augmentation promotes fat synthesis in these adipocytes and promotes the growth and accumulation of adipose tissue is necessary.
Therefore, promoting fat synthesis is important as a means of forming a feminine “fluffy” contour and breast augmentation. In addition, the fat synthesis promoter according to the present invention is present in various extracts of each plant, has an extremely excellent fat synthesis promotion effect, and has stable properties. Therefore, the various plant extracts having the effect of promoting fat synthesis according to the present invention can be used in industrial fields such as pharmaceuticals and foods.

That is, the fat synthesis promoter and the cosmetic according to the present invention are prepared from Kawakawa (Piper methys).
ticum Forst. ), Kumatsudura (Verbe)
naofficinalis L .; ), Kohakobe (St
ellaria media (L.) Vill. ), Taraxacum officin
ale Weber, Taraxcacum sec
t. Ruderaria species), Filippodia ulmari (Filipendura ulmari)
a (L.) Maxim. ), Sunflower (Heliant)
hus annuus L .; ), Majorana (Origa
num majorana L .; ), Trifolium platenense L., Ratani (Krameria triandra Rui)
z et Par. ), Larrea tri
dentata, Larrea mexicana), characterized by a fat synthesis promoting effect comprising an extract of one or more plants selected from the group consisting of:

For example, one of the various active ingredients according to the present invention
Seeds or two or more kinds are blended with various cosmetic bases and the like, and various basic cosmetics such as creams, emulsions, lotions, packs, face wash, foundations, various make-ups such as blush, lipstick, white powder, It can be widely applied to other cosmetics such as soap and cologne. In addition, the above-mentioned various aspects of cosmetics can be applied in various aspects such as solutions, emulsions, ointments, oils, waxes, sols, gels, powders, and sprays.

[0027]

As described above, various extracts of each plant according to the present invention provide a cosmetic composition containing one or more kinds of safe and stable fat synthesis promoters without side effects as active ingredients. Therefore, it is possible to achieve a feminine “plump” contour, especially breast augmentation.

[0028]

EXAMPLES Next, the present invention will be described in more detail by way of examples, but the present invention is not limited to these examples. In addition, the part in an Example shows a weight part unless there is particular notice.

1. Preparation Examples of Various Plant Extracts (1) Preparation Example 1 (Water Extract): Active Substances 2 to 5 of Each Plant
g was placed in a cylindrical filter paper, immersed in 50 to 100 ml of ion-exchanged water, and heated and extracted at 60 ° C. for 8 hours to obtain a filtrate. This operation was repeated three times, and all the liquids were combined and freeze-dried to obtain a water extract of each plant. (2) Preparation Example 2 (Various lower alcohol extracts): After extracting various lower alcohols for 8 hours using a Soxhlet extractor, the solvent was distilled off, and the extract was powdered to obtain various extracts of each plant. A lower alcohol extract was obtained. (3) Preparation Example 3 (extract of various organic solvents or organic solvent aqueous solution): In the extraction operation of the water extract, various organic solvents or organic solvent aqueous solutions were used instead of water. All extracts were combined, the solvent was distilled off as much as possible, concentrated, and then lyophilized to obtain extracts of various organic solvents or aqueous organic solvent of each plant.

2. First, (1) DMEM medium containing 10% by volume of CS, (2)
Prepare a differentiation induction medium. (1) DMEM medium containing 10% by volume CS: 13.48 g of DMEM medium (manufactured by IBC Biomedicals)
, 1 L of distilled water was added, and the final concentration was 10% by volume CS (C
ALF BOVINE SERUM), 0.1 wt% sodium bicarbonate, 70 mg / L streptomycin sulfate and 70 mg / L potassium benzylpenicillin. (2) Differentiation induction medium: DMEM medium (IBC Biom)
1L of distilled water to 13.48 g)
Each with a final concentration of 10% by volume CS (CALF BOVIN
E SERUM), 10 μg / mL insulin, 0.
5 mmol / L 1-methyl-3-isobutylxanthine (MIX), 0.25 μmol / L dexamethasone (D
EX), 0.1% by weight sodium bicarbonate, 70 mg /
Prepared by adding L streptomycin sulfate and 70 mg / L potassium benzylpenicillin.

Adipocyte precursor cell line (mouse 3T3-L-
1: Dainippon Pharmaceutical Co., Ltd.) was adjusted to 1 × 10 ⁵ cells / mL in a DMEM medium containing 10% by volume of CS, and a 6-well plate (I
2 mL each, and cultured at 37 ° C. under 5% carbon dioxide gas and saturated steam.

Each sample (about 1% by weight of pure content) was added to 10% by volume.
The mixture was diluted with a CS-containing DMEM medium to give a 1% by volume sample-supplemented solution (about 0.1 mg / mL pure). In addition, a DMEM medium containing 10% by volume of CS as a blank, β-estradiol as a positive control, and soybean and cuckoo extracts as comparative controls were subjected to the test.

After confient (about 3 days), the culture medium was removed by suction, and the cells were washed once with PBS (-).
1.8 mL of differentiation induction medium, 0.2 mL of each sample added solution
Was added, and differentiation induction / culture was performed for 2 days.

After 2 days of differentiation induction / culture, the culture solution is removed by suction, and the cells are washed once with PBS (-). next,
The fat cells were detached with a 0.25% by weight trypsin solution, the supernatant was removed, and then the fat cells were collected with PBS (-) to obtain a test solution.

After isopropanol is added to the test solution and mixed, phospholipids, sugars and free glycerin exhibiting a similar color are adsorbed on an adsorbent (activated alumina) and centrifuged to obtain triglyceride as a supernatant. Next, 5% potassium hydroxide was added to the triglyceride to saponify it, and glycerin was released. Then, a buffer solution (2.4 mol / L ammonium acetate buffer (pH 5.5), 11% by weight isopropanol) was added to adjust the pH to 6, and Glycerin is oxidized with a 50 mmol / L sodium metaperiodate solution. As a result, one molecule of formic acid and two molecules of formaldehyde are produced from glycerin. The formed formaldehyde reacts with acetylacetone and ammonia in the buffer to form a cyclic compound (3,5-diacetyl-1,4-dihydrolutidine), which becomes a yellow solution (measurement solution) having an absorption maximum at 410 nm.

Transfer the measurement solution to a 96-well microplate,
Plate reader: The absorbance of the sample at 410 nm (Es) and the absorbance of the blank (Eb) were measured.
The fat synthesis promoting effect of the various extracts of each plant according to the present invention was calculated by calculating the fat synthesis promoting rate (%) according to Equation 1. The results are shown in Table 1.

[0037]

(Equation 1)

[0038]

[Table 1]

From these results, various extracts of each plant according to the present invention have an effect of promoting fat synthesis.

3. Example of Formulation of Cosmetic The cosmetic according to the present invention was prepared using various extracts of each plant according to the present invention. The mixing ratio is part by weight.

(1) Formulation Example 1 (Lotion) [Production method] The raw materials are added to purified water and mixed uniformly.

(2) Formulation example 2 (cosmetic cream) [Manufacturing method] The raw materials for the aqueous phase are mixed and heated to 70 ° C. to form an aqueous phase. On the other hand, the raw materials of the oil phase are mixed and dissolved by heating to 70 ° C. to obtain an oil phase portion. This oil phase is added to the above-mentioned aqueous phase to carry out preliminary emulsification, homogenize uniformly with a homomixer, and cool to 30 ° C. to obtain a cosmetic cream.

(3) Formulation example 3 (cosmetic emulsion) [Manufacturing method] The raw materials for the aqueous phase are mixed and heated to 70 ° C. to form an aqueous phase. On the other hand, other raw materials are mixed and dissolved by heating to 70 ° C. to obtain an oil phase. This oil phase is added to the above-mentioned aqueous phase and emulsified, and cooled to 30 ° C. to obtain a cosmetic emulsion.

(4) Formulation Example 4 (Packing agent) [Production method] The raw materials of the aqueous phase are mixed and made uniform. Further, other raw materials are mixed and stirred until the mixture becomes uniform to obtain a pack.

(5) Formulation Example 5 (creamy foundation) [Production method] A part of the oil phase and the powder are put on a three-roll mill, and the remaining oil phase is added and dissolved by heating, and kept at 80 ° C. Next, the aqueous phase dissolved by heating is gradually added, and the mixture is emulsified at 80 ° C., and cooled to room temperature with stirring to obtain a creamy foundation.

4. Example of Effect Test of Cosmetic Further, the effect of the cosmetic according to the present invention when actually used was examined.

The rattany (Krameria) of Formulation Example 2
triandra Ruiz et Par. ) About the cosmetic cream containing the extract, 20 people of 16-30 years old who want to form a feminine "fluffy" outline and breast augmentation are panelists, twice a day every morning for 1 month,
It was applied to the face and the whole breast, and a use test was performed. Controls include rattanii (Krameriatori).
andra Ruiz et Par. ) A placebo cream excluding the extract was formulated in the same manner. In addition, the evaluation method was evaluated based on the following criteria. The results are as shown in Table 2, and the numbers in the table represent the number of people. No one complained of skin abnormalities during the use period.

[Evaluation Criteria] Effective: Clearly feminine “fluffy” contour formation and breast augmentation could be felt. Slightly effective: I was able to feel the formation of a "fluffy" contour that was somewhat feminine and breast augmentation. Invalid: No change from before use.

[0049]

[Table 2] Forming effect of feminine "fluffy" contour

[Table 3] Breast augmentation effect of breast

From the above results, it can be seen that the cosmetic according to the present invention has a feminine “fluffy” contour formation and a breast augmentation effect.

[0052]

As described above, it is possible to provide an excellent fat synthesis promoter and cosmetic based on the fat synthesis promoting effect of various extracts of each plant. Moreover, since the fat synthesis promoter is a natural product contained in various extracts of each plant, it is safe with little side effects and is stable to heat and the like. It can be expected that a remarkable effect to achieve the object of the invention will be achieved, such as opening up a wide range of technical fields.

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Claims (2)

[Claims] 1. Kawakawa (Piper method)
icum Forst. ), Kumatsudura (Verben)
a officinalis L .; ), Kohakobe (St
ellaria media (L.) Vill. ), Taraxacum officin
ale Weber, Taraxacum sec.
Ruderalia species), Filippodia ulmaria
(L.) Maxim. ), Sunflower (Helianth)
us annuus L. ), Majorana (Origan)
um majorana L. ), Trifolium platenense L., Ratani (Krameria triandra Rui)
z et Par. ), Larrea tri
A fat synthesis promoter comprising one or more plant extracts selected from dentata, Larrea mexicana). 2. Kawakawa (Piper method)
icum Forst. ), Kumatsudura (Verben)
a officinalis L .; ), Kohakobe (St
ellaria media (L.) Vill. ), Taraxacum officin
ale Weber, Taraxacum sec.
Ruderalia species), Filippodia ulmaria
(L.) Maxim. ), Sunflower (Helianth)
us annuus L. ), Majorana (Origan)
um majorana L. ), Trifolium platenense L., Ratani (Krameria triandra Rui)
z et Par. ), Larrea tri
A cosmetic comprising one or more plant extracts selected from dentata, Larrea mexicana).