KR20020076375A - Cosmetics Useful for atopic skin - Google Patents

Abstract

PURPOSE: A cosmetic containing ceramide, aloe vera, green tea extracts, Scutellaria baicalensis George extracts as an effective ingredient is provided which is effective for improvement of dermatoxerasia, pruritus and erythema of atopic skin. CONSTITUTION: The cosmetic contains one or more natural products selected from the group consisting of ceramide, aloe vera, green tea extracts and Scutellaria baicalensis George extracts, wherein the green tea extracts and Scutellaria baicalensis George extracts exhibit antibacterial activity against skin bacteria, the aloe vera, green tea extracts and Scutellaria baicalensis George extracts inhibit prostaglandin formation, and the ceramide has a moisturizing effect.

Description

Cosmetics useful for atopic skin {Cosmetics Useful for atopic skin}

The present invention relates to natural products effective for atopic skin and cosmetics containing the active ingredients thereof. More specifically, the present invention comprises a ceramide, aloe vera, green tea extract, golden extract as an active ingredient, and relates to a cosmetic that improves the dryness of atopic skin and is effective in normalizing skin fungus.

Atopic dermatitis is a disease characterized by severe itching and chronic skin rash. It is often called febrile fever and is the most common form of infant eczema. Although its cause is not yet clear, about 70% of patients with atopic dermatitis had a history of atopic dermatitis, and it is called in allergic congenitals, especially with allergic rhinitis and asthma. have. The disease is characterized by the initial severe itching, which is most likely to be aggravated by scratching the skin and subsequent secondary infections, especially the patient's mental stability and emotional state.

In the field of atopic dermatitis treatment, various medicines have been developed and used, but the selection of cosmetics for proper skin care is only a highly moisturizing product, but there are still many areas that are insufficient.

For atopic dermatitis, steroids, antihistamines, corticosteroids, and antibiotics are used in secondary infections, but they are mainly used in pharmaceuticals, and in cosmetics, effective substances are used because they have excellent moisturizing effects such as oils and high moisturizing factors. This is mainly a concept to relieve dryness of atopic skin rather than the treatment of diseases. In addition, according to a recent research report that ceramide (ceramide), which is one of skin keratinocyte lipids, is reduced in atopic skin compared to normal skin, there is a cosmetic product for improving skin barrier function through ceramide. However, these raw materials rely only on existing moisturizing effects, and the effects of improving the main symptoms of atopic skin such as itching are insignificant.

That is, until now, cosmetics have been mainly developed to alleviate dryness among the various symptoms of atopic skin described above, and its healing effect is insignificant, and development of cosmetic raw materials with antibacterial activity against Staphylococcus aureus is insufficient.

Therefore, the present inventors have intensively studied to reduce the dryness of atopic skin, as well as to suppress erythema through anti-inflammatory action, and to develop cosmetics containing antibacterial substances against skin fungus. As a result, ceramide reduces the dryness and aloe vera. The green tea extract, the anti-inflammatory effect of the golden extract, through the antimicrobial effect of the green tea extract was found to be effective in improving atopic skin, thereby completing the present invention.

That is, an object of the present invention is to provide an effective cosmetic for atopic skin containing ceramide, aloe vera, green tea extract, and golden extract.

Cosmetics effective for atopic skin according to the present invention for achieving the above object is characterized in that it contains at least one selected from the group consisting of gold, green tea, aloe vera and ceramide of natural products.

Cosmetics according to the present invention is prescribed to effectively maintain the skin moisture in the composition. In addition, aloe vera, green tea extract, golden extract, antimicrobial green tea extract, etc., which is an anti-inflammatory substance, is mixed to form an effective ingredient composition for atopy.

Natural products used in the cosmetic for atopy according to the present invention are as follows.

1) Aloe Vera

Scientific name: Aloe vera

Plant: A perennial herb, stems are very short. The leaves gather at the end of the stem and are succulent. The juice is called presbyopia and is used as a herbal medicine.

Ingredients: The juice contains alloin, coumalic acid, protein and a lot of calcium.

Action: Aloe juice is effective for the treatment of wounds and lacerations of the skin. It has also been found to have anticancer activity.

Application: Used in allergic rhinitis, fever, etc. in the treatment of sinus.

2) golden

Scientific name: Scutellaria baicalensis Georgi

Plants: perennial vegetation, 25-60cm high, stems squared, many branches at bottom. The root part is golden used as a herbal medicine.

Ingredients: Contains baicalein, baicalin, wogonoside.

Action: It is an antipyretic, anti-inflammatory, anti-allergic effect, and inhibits blood pressure.

Application: According to agreement, it is used for vomiting and bleeding in the nose area.

3) green tea

Scientific name: Camellia sinensis

Plant: A perennial vegetation, green tea is the young and tender leaf of the tea tree. Usually leaves can be harvested after 3 years.

Ingredients: Contains 1-5% of caffeine and purine alkaloids, and contains large amounts of tannins and flavonoids.

Action: Antioxidant and anti-inflammatory effects, antibacterial, diuretic and anticancer effects have been reported.

Application: Used for headache, malaria, etc. in motion.

In order to determine the effective ability of the raw material for atopic skin, the following test was carried out and the present invention will be described in more detail with reference to the following examples. These examples are intended to illustrate the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention.

Example 1: Preparation of Extract

The green tea and gold, which were well dried, were ground and extracted using ethanol or hot water as follows.

First, in the preparation of hot water extract, 500 ml of distilled water was added to dried golden 20 to 80 g and left at room temperature for 24 hours, then boiled for 30 minutes, filtered using a filter paper, and centrifuged at 8,000 × g for 15 minutes. Only drunk. The supernatant was then concentrated under reduced pressure on a 60 ° C. water bath with a rotary vacuum evaporimeter and lyophilized with a freeze dryer.

Ethanol extract was prepared as follows. In other words, 500 g of 80% ethanol was added to 20 g of dried green tea and golden sample, which was immersed in a 0 ° C. thermostat for 18 hours, the extract was filtered through a filter paper, and centrifuged at 8,000 × g for 15 minutes, and the supernatant was rotated by a rotary vacuum evaporator. It was concentrated under reduced pressure and lyophilized.

Example 2 Antibacterial Activity Test against Staphylococcus aureus

1. Test Methods and Results

Antimicrobial activity test against Staphylococcus aureus was done by liquid media dilution method. After diluting each sample in 100 ml intervals from 0 to 1000 ppm in 2 ml of liquid nutrient medium, 20 μl of 106 / ml of the bacterial solution was inoculated and incubated for 24 hours. The minimum concentration at which growth was inhibited based on turbidity was defined as the minimum growth inhibition concentration (MIC).

The results are shown in Table 1.

Antimicrobial Activity Test Results for Staphylococcus aureus sample Minimum growth inhibition (ppm) green tea 200-300 Gold 400-500

As shown in Table 1, it can be seen that gold, green tea is particularly excellent antibacterial activity against Staphylococcus aureus.

Example 3: Anti-inflammatory Test

1. Test Methods and Results

The anti-inflammatory effect was evaluated by the inhibitory effect of the production of prostaglandins. The effects of macrophages were measured using the extracted extracts. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500 uM to irreversibly inhibit the COX (cyclooxygenase) activity remaining in the cells. The suspension was then incubated for 2 hours in an incubator at 37 ° C. with 5% CO 2 in 100 ul of each well of a 96 well cell culture tube (96 well plate) to attach macrophages to the vessel surface. The attached macrophages were then washed three times with PBS and then used for the effect test of the extract.

After incubation for 12 hours by adding RPMI medium containing 1% (w / v) of LPS to 5 × 104 cells / ml of electrocultured macrophages, prostaglandins were produced and 100ul of the extract was treated to release prostaglandins. Was quantified using an enzyme immunoassay (ELISA). At this time, the production inhibitory activity of the prostaglandin of the extract was set to 100% the difference between the prostaglandin produced in the LPS treated group and the untreated group and compared with the control group through the percentage of prostaglandin reduced by treating the LPS and the sample together. , And the results are shown in Table 2 below.

Inhibitory Effect of Prostaglandins Blank Control group (Aspirin treated group) Green Tea 0.01% 0.01% golden Aloe 0.01% 100.0% 23.4% 34.6% 42.2% 31.8%

Example 4 Primary Skin Stimulation Test

From the results of Test Example 1-3, it can be seen that aloe vera, gold, and green tea have antimicrobial activity against erythema reaction and skin fungus caused by atopic inflammation, and whether the natural products are harmful to human body when applied to cosmetics. Human skin stimulation test was conducted to determine whether or not.

Human skin primary stimulation test was a human patch test (human patch test). That is, 30 healthy women aged 20-30 years old were tested according to CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 20 μl of the test substance (10% in patch base) prepared in the Finn Chamber was added dropwise, washed with 70% ethanol, dried, and fixed on the back of the test site with a micropore tape. . The patch is applied for 24 hours, and after removing the patch, the marking area is marked with a marking pen. After 30 minutes and 24 hours, the skin reaction of each test site is observed and the International Contact Dermatitis Research Group ; ICDRG). The results are shown in Table 4.

In Table 4, the average reactivity was calculated according to Equation 2 below, ± was 1 point, + was 2 points, ++ was 3 points, and +++ was 4 points. The criterion was based on less than 1 grade I (non-irritating range), less than 3 grade II (light stimulation range), less than 5 grade III (medium stimulation range), and 5 or more grade IV (strong stimulus range). .

Test substance 24 hours 48 hours Average reactivity (n = 30) Judgment grade ± + ++ +++ ± + ++ +++ Atopy base 5 - - - One - - - 2.50 Ⅱ Base + green tea 0.4% 6 - - - One - - - 2.92 Ⅱ Base + Golden 0.4% 6 - - - - - - - 2.75 Ⅱ Base + Aloe Vera 0.4% 6 - - - - - - - 2.75 Ⅱ Base + Golden 0.2% + Green Tea 0.2% + Aloe Vera 0.2% 6 - - - - - - - 2.75 Ⅱ

As shown in Table 3, atopy bases exhibited a safe range of stimulus values, but tended to be somewhat higher. All raw materials showed grade II or higher stimulation at the test prescription. In the case of a combination of various ingredients, the level of safety was Grade II (less than 3 stimulus level).

[Example 1-25]

After the preparation of the cosmetics containing natural products in the compositions of Tables 4 and 5, the changes in skin moisture content, efficacy evaluation and side effects were evaluated for the prepared cosmetics, respectively, and the results are shown in Table 6.

Evaluation of Skin Moisture Change: The change of skin moisture content was evaluated through the increased ratio by comparing the results before and 1 month after treatment with a corneometer.

Efficacy evaluation: The patient's questionnaire was evaluated before the sample treatment, and the degree of itching and erythema was assessed. The validity of the sample was evaluated through the percentage of subjects. For example, an effective 50% means that the itch or erythema is 50% improved in at least three stages among all the subjects.

Side effects evaluation: The presence or absence of side effects such as irritation through the survey of the subjects, and the dermatologist, the evaluator, directly evaluated the skin condition of the subjects to determine the side effects.

Furtherance content(%) Stearic acid 1.20 Eldew CL-301 0.60 Cetanol-k 2.00 GMS # 105 1.20 Crodalan SWL 1.50 Wecobee SS 1.50 Arl # 165 1.50 Myrj # 52s 1.70 Arl # 83 0.40 D-M 0.20 D-P 0.10 MDF 4.00 ODO 5.00 CEH 5.00 DC 200F 0.50 Vitamin E acetate 0.50 Ceramide PC 104 1.20 Cholesterol 0.50 Linoleic acid 1.00 C6-PS 0.30 Keramide 0.00 DC 345 5.00 Fragrance 0.10 Dl-water to 100 EDTA-2Na 0.02 Glycerin 6.00 1,3 BG 4.00 PG 3.00 Bio-HE 5.00 SC-glucan 5.00 Flocare ET-58 0.60

0 in Table 5 indicates that a natural product labeled 0 is additionally used in the composition of Table 4. In addition, the amount of natural products used in each of Examples 1 to 7 is golden 0.2%, green tea 0.2%, aloe vera 0.2% and 8 to 14 is golden 0.3%, green tea 0.5%, aloe vera 0.5%.

Natural products Example One 2 3 4 5 6 7 8 9 10 11 12 13 14 Gold ○ ○ ○ ○ ○ ○ ○ ○ green tea 0 0 ○ ○ ○ ○ ○ ○ Aloe ○ ○ ○ 0 0 ○ ○ ○

Example Skin moisture change Effectiveness evaluation Side effects assessment One 10% increase 35% Weak erythema, itching 2 10% increase 25% Weak erythema, itching 3 10% increase 43% Weak erythema, itching 4 10% increase 35% Weak erythema, itching 5 10% increase 20% Weak erythema, itching 6 10% increase 26% Weak itching 7 10% increase 20% Weak erythema, itching 8 10% increase 46% Weak erythema, itching 9 10% increase 48% Weak erythema, itching 10 10% increase 60% Weak erythema, itching 11 10% increase 50% Weak erythema, itching 12 10% increase 47% Weak erythema, itching 13 10% increase 38% Weak erythema, itching 14 10% increase 43% Weak erythema, itching

As shown in Table 6, the case of Example 10 was the most ideal, the higher the concentration of natural products contained was found to have a higher effect of improving the erythema and itching.

As described and demonstrated above, the present invention includes a green tea, golden, aloe vera and ceramide and an electric extract or a mixture thereof as an active ingredient and provides a cosmetic that is effective in improving dryness, itching and erythema of atopic skin . Green tea and gold of the present invention exhibit antimicrobial activity against skin fungus, aloe vera and green tea, gold inhibits the production of prostaglandins, ceramide has a moisturizing effect. In addition, it was confirmed that the extract or the mixture of the present invention is safe when applied to the actual skin, the extract or the mixture of the present invention as an active ingredient of cosmetics for the improvement of dryness and itching, erythema of atopic skin It may be useful.

Claims (1)

Cosmetics effective for atopic skin, comprising at least one natural product selected from the group consisting of gold, green tea, aloe vera and ceramide among natural products.