KR20220011331A - Cosmetic composition for improving atopic dermatitis and method of manufacturing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating atopic dermatitis, comprising as active ingredients: a polydeoxyribonucleotide; a mixture of Aloe DNA extract, Castrodia elata Blume DNA extract and Centella asiatica DNA extract; and an extract of Centella asiatica, and to a method for preparing the same.

Description

Cosmetic composition for improving atopic dermatitis and method of manufacturing the same

The present invention relates to a cosmetic composition for improving atopic dermatitis, and more particularly, polydeoxyribonucleotide; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And it relates to a cosmetic composition for improving atopic dermatitis comprising an extract of Centella asiatica as an active ingredient and a method for preparing the same.

Atopic dermatitis (Atopic dermatitis) is a chronic recurrent eczematous disease, the cause and pathogenesis are not clearly identified. It is currently understood that atopic dermatitis is mainly caused by a unique genetic predisposition, but the cause is not simple and is attributed to a number of factors.

Various external environmental factors involved in the exacerbation of atopic dermatitis are well known, and the genetic predisposition and clinical and immunological forms of atopic dermatitis patients and their families are currently weak.

Immunological abnormalities due to genetic predisposition have been understood as the main etiology, and immunological abnormalities such as increase in blood IgE, increase in IL-4 and IL-5, and decrease in INF-γ have been suggested as evidence.

In general, atopic dermatitis is a skin inflammatory disease that takes a chronic course, and exhibits extreme pruritus and immunological abnormalities. More aggressive treatment is required in children because it can cause psychological problems such as secondary infection, lichenification, sleep disturbance, depression, inferiority complex, and psychological atrophy caused by unsightly appearance.

However, dry skin, the most characteristic clinical finding, is not explained by an immunological abnormality, and it is not clear whether it is caused by genetic factors or secondary changes in atopic dermatitis.

As a prior art related to a cosmetic composition for improving or treating atopic dermatitis, a cosmetic composition for improving atopic dermatitis comprising at least one of tetrahydrodiferuloylmethane and curcumin (Patent Document 1), gold, green tea, aloe vera and ceramide Cosmetics effective for atopic skin containing at least one natural product selected from the group consisting of (Patent Document 2), a skin cosmetic comprising a functional composition for improving atopic dermatitis containing a plant cell fermentation nano extract as an active ingredient (Patent Document 3), etc. This is disclosed.

However, in the prior art, a cosmetic composition for improving atopic dermatitis comprising polydeoxyribonucleotides (PDRN), aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract, and Centella asiatica extract is not known. it is not the case.

Republic of Korea Patent Publication No. 10-2018-0045539 Republic of Korea Patent Publication No. 10-2002-0076375 Republic of Korea Patent Publication No. 10-1835486

An object of the present invention is polydeoxyribonucleotide; Aloe DNA extract, Chunma DNA extract and Centella asiatica DNA extract; And by including the Centella asiatica extract as an active ingredient, to provide a cosmetic composition excellent in the treatment effect of atopic dermatitis.

In addition, the present invention provides a polydeoxyribonucleotide; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And by including a mixture of Centella asiatica extract as an active ingredient, to provide a method for preparing a cosmetic composition excellent in the treatment of atopic dermatitis.

The present invention provides a polydeoxyribonucleotide; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And it provides a cosmetic composition for improving atopic dermatitis comprising an extract of Centella asiatica as an active ingredient.

In addition, the present invention comprises the steps of preparing an aqueous component by dissolving a water-soluble component containing betaine in water; adding an oil-based component including macadamia seed oil to the water-based component and mixing by stirring; And in the mixture of the water-based component and the oil-based component, polydeoxyribonucleotide; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And it provides a method for preparing a cosmetic composition for improving atopic dermatitis comprising the step of adding and mixing the Centella asiatica extract.

According to the present invention, polydeoxyribonucleotides; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And by including the Centella asiatica extract as an active ingredient, it is possible to provide a cosmetic composition excellent in the treatment effect of atopic dermatitis.

1 is a photograph showing the state before using the cosmetic composition for improving atopic dermatitis of the present invention.
2 is a photograph showing the state after 45 days of using the cosmetic composition for improving atopic dermatitis of the present invention.
3 is a photograph showing the state after 90 days of using the cosmetic composition for improving atopic dermatitis of the present invention.

The present invention relates to polydeoxyribonucleotides; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And it relates to a cosmetic composition for improving atopic dermatitis comprising an extract of Centella asiatica as an active ingredient.

The Centella asiatica extract included in the cosmetic composition for improving atopic dermatitis of the present invention is a water-insoluble extract and is known as a wound healing and anti-wrinkle substance.

The Centella asiatica extract is an organic solvent extract of Centella asiatica (L.) Urban. belonging to the Umbelliferae, preferably an organic solvent selected from alcohol, acetic acid, propylene glycol, ethylene glycol, butylene glycol and dioxane. Extracts, more preferably those prepared by purifying the ethanol extract may be used.

In the cosmetic composition for improving atopic dermatitis of the present invention, the Centella asiatica extract is a highly concentrated pure powder raw material obtained by separating and purifying only the Triterpenic part of Centella asiatica, and the Triterpenic part is Asian Since it is composed of components of Asiatic acid, Madecassic acid and Asiaticoside, it is preferable to use a quantitative extract extracted with high concentration of only the core component.

The polydeoxyribonucleotide (PDRN) contained in the cosmetic composition for improving atopic dermatitis of the present invention is a material extracted from testes of salmon and trout, and is a DNA fragment having a composition most similar to that of the human body, wherein the PDRN is water. It is soluble in a very small amount in a solvent, and insoluble in organic solvents such as alcohol and acetone.

The PDRN is a substance that promotes cell regeneration and quickly recovers wounds while reducing scar formation and pain as well. It is used to treat ulcers and promote tissue regeneration after surgery.

In addition, PDRN is used according to the judgment of the medical staff for some tissue regeneration or inflammation treatment for which there is no suitable treatment. Also, it stimulates the A2 receptor, which is a signal for skin regeneration, to promote secretion of various growth factors, and vascular endothelial cell growth factor (VEGF) causes capillaries. It is known as a component that produces blood vessels, improves blood circulation, has anti-inflammatory action and prevents capillary leakage.

In the cosmetic composition for improving atopic dermatitis of the present invention, the molecular weight of the polydeoxyribonucleotide may be 20Da to 10,000kDa, but it is preferable to use molecularized PDRN having a molecular weight of 2,000kDa or less.

In general, in the case of isolating proteins or DNA from microorganisms and animal cells, since the microorganisms and animal cells are composed of cell membranes, it was possible to form a kit in which the separation and purification process consisted of only a chemical reaction without a physical grinding process. On the other hand, in the case of plants, because of the cell wall composed of cellulose, cell lysis does not occur only through a simple chemical reaction.

Therefore, the plant dried using a grinder is ground into powder through a grinding operation. Since the degree of pulverization is closely related to the extraction time, the finer the pulverization, the larger the surface area and the larger the contact area with the DNA extraction solution. This means that a lot of extraction occurs even in a short time, and the actual extraction time is also prolonged because the transmittance is lowered.

When several examples were conducted according to the concentration of salt obtained from the deep sea water suitable for each target, the most DNA was extracted when 3 to 10% of the raw material was added to the salt obtained by drying the deep sea water and each plant DNA was extracted. The reason for using deep sea water at all times is to obtain clean salt rich in inorganic nutrients because sunlight does not reach it and there are almost no organic matter and pathogens under the temperature condition maintained at 2℃.

The DNA released through the cell membrane is ^{combined with Na +} of salt and then treated with ethanol (1.5 to 2.5 times the volume of the cell extract).

After centrifuging the DNA condensed in the ethanol layer several times, the DNA precipitate is purified once more with purified water to obtain plant DNA.

Aloe is a shrub succulent plant belonging to the Liliaceae family, and the juice of the raw leaves is taken internally for gastrointestinal diseases, externally applied to trauma and burns, etc. have.

The aloe DNA extract included in the cosmetic composition for improving atopic dermatitis of the present invention is obtained by selecting an aloe raw material and setting the concentration with a salt extracted from deep sea water suitable for the aloe raw material by extracting, separating and purifying the DNA. manufactured can be used.

Gastrodia elata BLUME) is a perennial parasitic plant that parasitizes the mycelium of mushrooms growing on the rotten stump of an oak tree. It is used to treat diseases such as incontinence, speech disorders, high blood pressure, epilepsy in children, and epidemic meningitis.

The Chunma DNA extract included in the cosmetic composition for improving atopic dermatitis of the present invention is extracted, separated and purified by setting the concentration with the salt extracted from the deep sea water suitable for the Chunma raw material after selecting the Chunma raw material. manufactured can be used.

In addition, the Centella asiatica DNA extract included in the cosmetic composition for improving atopic dermatitis of the present invention is a salt extracted from deep sea water suitable for the Centella asiatica raw material after selecting the Centella asiatica raw material. One prepared by extracting, separating and purifying DNA by setting the concentration may be used.

The mixture of the aloe DNA extract, Chunma DNA extract, and Centella asiatica DNA extract included in the cosmetic composition for improving atopic dermatitis of the present invention is 1:0.1 A weight ratio of ~10:0.1 to 10, for example, a mixture in a weight ratio of 1:1:1 may be used, but the present invention is not limited thereto.

The cosmetic composition for improving atopic dermatitis of the present invention comprises: the polydeoxyribonucleotide as an active ingredient; and a mixture of Aloe DNA extract, Chunma DNA extract and Centella asiatica DNA extract; And Centella asiatica extract may include 0.1 to 10% by weight, preferably 0.5 to 5% by weight, more preferably 1 to 2% by weight relative to the total weight of the composition.

When the active ingredient is less than 0.1% by weight relative to the total weight of the composition, the predetermined purpose of treating atopic dermatitis cannot be achieved, and when it exceeds 10% by weight, it may be difficult to maintain the stability of the formulation.

The cosmetic composition for improving atopic dermatitis of the present invention contains 20 to 30 parts by weight of the Centella asiatica extract and 20 to 30 parts by weight of the polydeoxy based on 100 parts by weight of a mixture of the aloe DNA extract, the chrysanthemum DNA extract and the Centella asiatica DNA extract. It may contain 0.5 to 2 parts by weight of ribonucleotides.

The cosmetic composition for improving atopic dermatitis of the present invention preferably contains 23 to 27 parts by weight of the Centella asiatica extract and 23 to 27 parts by weight of the Centella asiatica extract based on 100 parts by weight of a mixture of the aloe DNA extract, the Chunma DNA extract and the Centella asiatica DNA extract. It may contain 0.75 to 1.5 parts by weight of polydeoxyribonucleotides.

The cosmetic composition for improving atopic dermatitis of the present invention more preferably contains 25 parts by weight of the Centella asiatica extract and 25 parts by weight of the poly 1 part by weight of deoxyribonucleotides

The formulation of the cosmetic composition for improving atopic dermatitis of the present invention may be a cream, lotion, lotion, essence, gel, serum, pack, powder, ointment for external use, suspension, emulsion, spray or cosmetic solution, but is not limited thereto.

The cosmetic composition for improving atopic dermatitis of the present invention preferably has a cream, lotion, external skin ointment or spray formulation, but the formulation can be modified as needed.

The cosmetic composition for improving atopic dermatitis of the present invention may further include an aqueous component including betaine and an oil-based component including macadamia seed oil, but is not limited thereto.

The cosmetic composition for improving atopic dermatitis of the present invention includes butylene glycol, glycerin, thickener, allantoin, xanthan gum, 1,2-hexanediol, ethylhexylglycerin, EDTA-2Na, panthenol, ceramide and hyaluron as the aqueous components. It may further include one or more components selected from among.

The glycerin (glycerin) suppresses the skin conditioning effect that enhances the vitality of the skin and the growth of anaerobic bacteria, and expresses a deodorizing effect.

The 1,2-hexanediol (1,2-hexanediol) has a surfactant structure having both a hydrophobic group and a hydrophilic group, and functions as a moisturizer and a preservative. Substances can be substituted, and a more excellent effect can be achieved by mixing with capryryl glycol in a weight ratio of 1:1.

The ceramide is a kind of sphingolipid having a structure in which a fatty acid is linked to sphingosine or phytosphingosine, and accounts for about 40% or more of the keratinocyte intercellular lipid constituting the stratum corneum of the skin, and plays a role in the formation or function of the structure of the stratum corneum. It is an essential component to show.

The cosmetic composition for improving atopic dermatitis of the present invention contains cetearyl alcohol, cetyl alcohol, beeswax, olive oil, hydrogenated polydecene, diisostearyl malate, cyprilic/capric triglyceride as the oil-based ingredients. It further contains at least one component selected from ceride, dimethicone, glyceryl monostearate (GMS), glyceryl stearate, glyceryl caprylate, and pentaerythrityltetra-di-t-butylhydroxyhydrocinnamate. can do.

The cosmetic composition for improving atopic dermatitis of the present invention further comprises a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water , ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or commonly used in cosmetics It may contain adjuvants commonly used in cosmetic or dermatological fields such as any other ingredients, but is not limited thereto.

In addition, the above ingredients may be introduced in an amount generally used in the field of dermatology.

In particular, when the cosmetic composition according to the present invention is in a cream formulation, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used, but is not limited thereto.

In addition, when the cosmetic composition according to the present invention is a spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and additionally, chlorofluorohydrocarbon, propane/ propellants such as butane or dimethyl ether, but are not limited thereto.

In addition, the present invention comprises the steps of preparing an aqueous component by dissolving a water-soluble component containing betaine in water, adding an oil-based component including macadamia seed oil to the aqueous component, and stirring to mix, and the aqueous component For improving atopic dermatitis comprising adding and mixing a mixture of Centella asiatica extract, polydeoxyribonucleotides, and aloe DNA extract, Chunma DNA extract and Centella asiatica DNA extract to a mixture of oil and oil-based ingredients It relates to a method for manufacturing a cosmetic composition.

The manufacturing method of the cosmetic composition for improving atopic dermatitis will be described in detail for each step.

In the step of preparing the aqueous component, for example, a water-soluble component including betaine may be dissolved in water at a temperature of 65°C to 85°C.

Butylene glycol, glycerin, thickeners (polyacrylate-13, polyisobutene, polysorbate 20, etc.), allantoin, xanthan gum, 1,2-hexanediol, ethylhexylglycerin, EDTA-2Na, It may further include one or more components selected from panthenol, ceramide NP, and hyaluron.

Next, after confirming that the water-soluble component in the water-based component is completely dissolved in water, an oil-based component including macadamia seed oil may be added to the water-based component and mixed at high speed.

The oil-based component may be prepared by heat treatment at a temperature of 70 to 90° C., and when two or more oil-based components are used, it may be prepared using a separate container.

The oil-based ingredients include cetearyl alcohol, cetyl alcohol, wax, olive oil, hydrogenated polydecene, diisostearyl malate, cyprilic/capric triglyceride, dimethicone, glyceryl monostearate, It may further include one or more components selected from glyceryl stearate, glyceryl caprylate, and pentaerythrityltetra-di-t-butylhydroxyhydrocinnamate.

In addition, the water-based component and the oil-based component may be homogenized by stirring at a speed of 4,000 to 6,000 rpm at a temperature of 75 to 80° C. for 20 to 30 minutes.

Then, polydeoxyribonucleotide (PDRN); Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; and adding and mixing a Centella asiatica extract, preferably a quantitative extract of Centella asiatica.

According to an embodiment of the present invention, polydeoxyribonucleotide (PDRN); Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And before adding and mixing the quantitative extract of Centella asiatica, the resultant mixture of the water-based component and the oil-based component is cooled to a temperature of 40 to 55° C., and again at a speed of 4,000 to 6,000 rpm for 10 to 20 minutes. can be further mixed with

In addition, when adding polydeoxyribonucleotide (PDRN), a mixture of the DNA extract, and a Centella asiatica extract, preferably a quantitative extract of Centella asiatica to the mixed result, the quantitative extract of Centella asiatica is organic It can be added dropwise after dissolving in a solvent (propylene glycol, alcohol, etc.) at a temperature of 50 to 60 °C.

In addition, optionally, polydeoxyribonucleotides (PDRN), a mixture of the DNA extract, and a mixture of a quantitative extract of Centella asiatica are added and mixed with ingredients commonly used in cosmetic compositions such as fragrances and extracts. More can be added. Of course, it is also possible to add conventional components of the cosmetic composition in the previous step.

Thereafter, the mixture may be further mixed for 10 to 30 minutes, and the pH and viscosity of the resultant mixture may be checked, cooled, and transferred to a packing step. If necessary, the pH may be adjusted to 5.5 to 7 with citric acid.

Hereinafter, the contents of the present invention will be described in detail through examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited thereto.

<Preparation Example 1> Preparation of Aloe DNA Extract

50 g of dried aloe plant leaves were placed in a 200 ml grinder and ground into powder for 30 minutes. Then, 50 g of aloe plant leaf powder was added to a solution in which 2.5 g of salt obtained from deep sea water (5% of the original water) was added to 100 ml of purified water.

After mixing slowly for 50 minutes at 100 rpm in a state of being placed in an azimuth mixer, residues were removed using a vacuum filtration device using 10 μm filter paper. 200 ml of ethanol was slowly added while slowly rotating the beaker containing the aloe cell extract from which the residue was removed at 100 rpm in an azimuth mixer.

After confirming the white condensed material in the ethanol layer, it was transferred to a 1.5 ml Eppendorf-tube and centrifuged 3 times at 4° C. at 12,000 rpm for 15 minutes each. After pouring the supernatant into another tube, centrifuge again at 4°C at 12,000 rpm for 15 minutes to dry the DNA precipitate, then purify with purified water once more, and centrifuge at 4°C at 12,000 rpm for 15 minutes to separate the supernatant. Transferred to a Dorp tube to obtain 10 mg of aloe DNA extract.

<Preparation Example 2> Preparation of Chunma DNA extract

50 g of dried cheonma was put into a 200 ml grinder and ground into powder for 30 minutes. Then, 50 g of Chunma powder was added to a solution in which 1.5 g of salt obtained from deep sea water (3% of the original water) was added to 100 ml of purified water.

After mixing slowly for 50 minutes at 100 rpm in a state of being placed in an azimuth mixer, residues were removed using a vacuum filtration device using 10 μm filter paper. 200ml of ethanol was slowly added while slowly rotating the beaker containing the Cheonma cell extract from which the residue was removed at 130rpm in an azimuth mixer.

After confirming the white condensed material in the ethanol layer, it was transferred to a 1.5 ml Eppendorf tube and centrifuged 3 times at 4° C. at 12,000 rpm for 15 minutes each. After pouring the supernatant into another tube, centrifuge again at 4°C at 12,000 rpm for 15 minutes to dry the DNA precipitate, then purify with purified water once more, and centrifuge at 4°C at 12,000 rpm for 15 minutes to separate the supernatant. Transferred to a Dorf tube to obtain 10mg of the Chunma DNA extract.

<Preparation Example 3> Preparation of Centella asiatica DNA extract

50 g of dried Centella asiatica was placed in a 200 ml grinder and ground into powder for 30 minutes. Then, 50 g of Centella asiatica powder was added to a solution in which 3.5 g of salt obtained from deep sea water (7% of the original water) was added to 100 ml of purified water.

After mixing slowly for 50 minutes at 150 rpm in a state of being placed in an azimuth mixer, residues were removed using a vacuum filtration device using 10 μm filter paper. The beaker containing the Centella asiatica cell extract from which the residue was removed was slowly turned at 100 rpm in an azimuth mixer, and 200 ml of ethanol was slowly added.

After checking the white condensed material in the ethanol layer, it was transferred to a 1.5 ml Eppendorf tube and centrifuged 3 times for 15 minutes at 12,000 rpm at 4°C. After pouring the supernatant into another tube, centrifuge again at 4°C at 12,000 rpm for 15 minutes to dry the DNA precipitate, then purify with purified water once more, and centrifuge at 4°C at 12,000 rpm for 15 minutes to separate the supernatant. 10mg of Centella asiatica DNA extract was obtained by transferring to a Dorf tube.

<Examples 1 to 3> (Preparation of cosmetic composition)

After heating and mixing the water-based component and the oil-based component at 75° C. with the composition and content (wt%) shown in Table 1 below, respectively, cooled to room temperature, and then the water-based component and the oil-based component were mixed at 75° C. at a rate of 5,000 rpm At a temperature of , stirred for 25 minutes to homogenize. As a thickener in Table 1 below, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer was used.

Then, polydeoxyribonucleotide (PDRN) to the homogenized result; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And after adding and mixing the quantitative extract of Centella asiatica and homogenizing, PDRN, a mixture of aloe DNA extract, Chunma DNA extract and Centella asiatica DNA extract, and a cosmetic composition of a cream formulation comprising the quantitative extract of Centella asiatica was prepared.

ingredient Example 1 Example 2 Example 3 Purified water 59.24 59.28 59.185 butylene glycol 6.3 6.3 6.3 glycerin 6.5 6.5 6.5 thickener 0.8 0.8 0.8 allantoin 0.1 0.1 0.1 xanthan gum 0.1 0.1 0.1 1,2-Hexynediol 0.8 0.8 0.8 Ethylenehexylglycerin 0.05 0.05 0.05 EDTA-2Na 0.03 0.03 0.03 panthenol 0.5 0.5 0.5 betaine 0.5 0.5 0.5 Ceramide NP 0.5 0.5 0.5 Hyaluronic Acid (1%) 0.1 0.1 0.1 cetearyl alcohol One One One ceryl alcohol One One One beeswax 0.3 0.3 0.3 olive oil One One One Hydrogenated polydecene 3 3 3 Diisostearylate 2 2 2 Caprylic/Capric Triglyceride Dimethicone 2 2 2 macadamia seed oil 2 2 2 dimethicone 2 2 2 G.M.S. 1.5 1.5 1.5 Arachel165 1.5 1.5 1.5 Arachel 60 0.6 0.6 0.6 Glyceryl Caprylate 0.5 0.5 0.5 Pentaerythrityltetra-di-t-butylhydroxy
hydrocinnamate 0.02 0.02 0.02 alcohol (ethanol) 3 3 3 Centella asiatica quantitative extract 0.25 0.20 0.30 polydeoxyribonucleotides 0.01 0.02 0.015 Oat kernel, rice bran complex extract One One One natural flower complex 0.5 0.5 0.5 Aloe, Chunma, Centella asiatica DNA extract One One One Spices 0.3 0.3 0.3 Sum 100 100 100

<Comparative Examples 1 to 3> (Preparation of cosmetic composition)

As in the composition and content (wt%) shown in Table 2 below, either the quantitative extract of Centella asiatica is not included (Comparative Example 1), or does not contain polydeoxyribonucleotide (Comparative Example 2), or Aloe DNA extract, A cosmetic composition of a cream formulation was prepared in the same manner as in Example 1 except that the mixture of Chunma DNA extract and Centella asiatica DNA extract was not included (Comparative Example 3).

ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Purified water 59.49 59.50 60.24 butylene glycol 6.3 6.3 6.3 glycerin 6.5 6.5 6.5 thickener 0.8 0.8 0.8 allantoin 0.1 0.1 0.1 xanthan gum 0.1 0.1 0.1 1,2-Hexynediol 0.8 0.8 0.8 Ethylenehexylglycerin 0.05 0.05 0.05 EDTA-2Na 0.03 0.03 0.03 panthenol 0.5 0.5 0.5 betaine 0.5 0.5 0.5 Ceramide NP 0.5 0.5 0.5 Hyaluronic Acid (1%) 0.1 0.1 0.1 cetearyl alcohol One One One ceryl alcohol One One One beeswax 0.3 0.3 0.3 olive oil One One One Hydrogenated polydecene 3 3 3 Diisostearylate 2 2 2 Caprylic/Capric Triglyceride Dimethicone 2 2 2 macadamia seed oil 2 2 2 dimethicone 2 2 2 G.M.S. 1.5 1.5 1.5 Arachel165 1.5 1.5 1.5 Arachel 60 0.6 0.6 0.6 Glyceryl Caprylate 0.5 0.5 0.5 Pentaerythrityltetra-di-t-butylhydroxy
hydrocinnamate 0.02 0.02 0.02 alcohol (ethanol) 3 3 3 Centella asiatica quantitative extract - 0.25 polydeoxyribonucleotides 0.01 - 0.01 Oat kernel, rice bran complex extract One One One natural flower complex 0.5 0.5 0.5 Aloe, Chunma, Centella asiatica DNA extract One One - Spices 0.3 0.3 0.3 Sum 100 100 100

<Experimental Example 1> (Treatment effect of atopic dermatitis)

For a total of 50 patients with atopic dermatitis, using the cosmetic composition of the cream formulation prepared in Example 1 and Comparative Examples 1 to 3, the experiment was conducted for each 10 patients, and for the remaining 10 patients, the cosmetic composition was used. The experiment was conducted as a control group that was not used.

As for the experimental method, after washing in the morning and evening, 5 ml of each test sample, a cosmetic composition, was applied to the affected area for 90 days, and the results were evaluated compared to before application. was treated with , almost cured (10 points), and the overall evaluation was divided into 5 stages, and the results are shown in Table 3 (unit: persons) below.

division completely
cured A lot of
improved usually almost improved
not done no improvement at all Sum average score 10 7.5 5 2.5 0 - - Example 1 9 One - - - 97.5 9.75 Comparative Example 1 - One 9 - - 52.5 5.25 Comparative Example 2 - 9 One - - 72.5 7.25 Comparative Example 3 - 7 3 - - 67.5 6.75 control - - - One 9 2.5 0.25

As shown in Table 3 above, PDRN; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And the cosmetic composition of Example 1 comprising a quantitative extract of Centella asiatica is a cosmetic composition that does not contain a quantitative extract of Centella asiatica (Comparative Example 1), and a cosmetic composition that does not contain polydeoxyribonucleotide (Comparative Example 2) ) and the cosmetic composition (Comparative Example 3) that does not contain a mixture of aloe DNA extract, Chunma DNA extract, and Centella asiatica DNA extract, it can be seen that the therapeutic effect of atopic dermatitis is very excellent.

<Experimental Example 2> (Visual observation of the therapeutic effect of atopic dermatitis)

As shown in FIG. 1 , a patient (male, 28 years old) with severe atopic dermatitis applied 5 ml each of the cosmetic composition of Example 1 to the affected area with atopic dermatitis after washing in the morning and evening. The photographed pictures are shown in FIGS. 2 and 3, respectively.

As shown in Fig. 2, it can be seen that the progress of treatment compared to Fig. 1 can be seen after 45 days of application, and as shown in Fig. 3, it can be seen that atopic dermatitis is completely cured after 90 days of application.

Although described with reference to preferred embodiments of the present invention as described above, those of ordinary skill in the art can view the present invention within the scope without departing from the spirit and scope of the present invention as set forth in the following claims. It will be understood that various modifications and variations of the invention are possible.

Claims (7)

polydeoxyribonucleotides; Aloe DNA extract, a mixture of Chunma DNA extract and Centella asiatica DNA extract; And a cosmetic composition for improving atopic dermatitis comprising an extract of Centella asiatica as an active ingredient. The cosmetic composition for improving atopic dermatitis according to claim 1, wherein the active ingredient is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition. The method according to claim 1, wherein 0.01 to 1 of the Centella asiatica extract based on 100 parts by weight of the mixture of the Aloe DNA extract, the Chunma DNA extract, and the Centella asiatica DNA extract. A cosmetic composition for improving atopic dermatitis, comprising parts by weight and 0.01 to 2 parts by weight of the polydeoxyribonucleotide. The cosmetic composition for improving atopic dermatitis according to claim 1, wherein the Centella asiatica extract is a quantitative extract of Centella asiatica. The cosmetic composition for improving atopic dermatitis according to claim 1, wherein the formulation of the composition is a cream, lotion, lotion, essence, gel, serum, pack, powder, external ointment, suspension, emulsion, spray or cosmetic solution. The cosmetic composition for improving atopic dermatitis according to claim 1, further comprising an aqueous component containing betaine and an oil-based component containing macadamia seed oil. preparing an aqueous component by dissolving a water-soluble component including betaine in water;
adding an oil-based component including macadamia seed oil to the water-based component, stirring and mixing; and
Atopy comprising adding and mixing a mixture of Centella asiatica extract, polydeoxyribonucleotide, and aloe DNA extract, Chunma DNA extract and Centella asiatica DNA extract to the mixture of the water-based component and the oil-based component A method of manufacturing a cosmetic composition for improving dermatitis.

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