JPWO2007148737A1 - Method for preparing plant extract, plant extract and use thereof - Google Patents

Abstract

Various activators and anti-aging agents derived from animal and plant systems have been found, but in reality, sufficient and stable effects have not been obtained that are industrially usable. An object of the present invention is to provide a plant-derived cell activator or anti-aging agent that has an excellent cell activation or anti-aging effect on cells and can sufficiently withstand long-term use. The present invention relates to a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. To be used for cell activators or anti-aging agents characterized by containing, and cosmetics and quasi-drugs (external preparations for skin, bath preparations, hair growth agents, etc.), foods and drinks, and pharmaceuticals containing these as active ingredients Provide a way.

Description

  The present invention relates to a substance that enhances the activation or anti-aging effect of various cells and a method for using the same.

  The present invention relates to a method for efficiently and mass-producing a plant extract from a plant, and the plant extract and use thereof.

  Individual aging and the various diseases that accompany this are closely related to the aging of all dividing cells (decrease in division rate and cell function). For example, the skin is dermis and the epidermis is composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin, collagen, and hyaluronic acid that support these extracellular skin structures. In particular, fibroblasts mainly control the synthesis and degradation of collagen and hyaluronic acid (Non-patent Document 1). In young skin, moisture retention, flexibility, and elasticity are ensured by maintaining the constancy of the interaction between these skin tissues, and the skin has a firm and glossy appearance and is kept fresh. However, aging, ultraviolet rays, drying, stress, etc. cause deterioration of the function of the extracellular matrix and fibroblasts in particular. As a result, the skin's flexibility and moisturizing function are reduced, and the skin loses its tension and gloss and becomes rough. Aging symptoms such as wrinkles and dullness occur. It is also known that the function of fibroblasts declines with age and the turnover rate of collagen and hyaluronic acid decreases (Non-patent Document 2).

  Searching for an activator and an anti-aging agent has been carried out for the purpose of preventing or suppressing aging at the cellular level. For example, connective tissue hydrolysates (Patent Document 1), thymus / spleen-derived water-soluble protein (Patent Document 2), bovine placenta extract (Patent Document 3) and the like are known as animal-derived cell activators. . Examples of plant-derived cell activators include sesame seeds, sayaks, capsicum, toki, dokudami, bakumondo (patent document 4), almonds, dandelion, elderberry, cucumber, assembly, sunflower, tonin, carrot, hop, mugweed, and yokuinin. (Patent Document 5), extracts from the genus Turmeric (Patent Document 6), and the genus Hanayasuri (Patent Document 7) are known. Some of these are pharmaceuticals as cell activators and anti-aging agents. Although it is used in quasi-drugs and cosmetics, there are very large individual differences, and it cannot be said that the effect is sufficient, and there has been no cell activator or anti-aging agent that exhibits a satisfactory effect. .

  Examples of substances having an action to promote extracellular matrix production include, for example, licorice leaf extract (Patent Document 8), lotus germ extract (Patent Document 9), hydrolysis, and the like, which have an action to promote the production of collagen or hyaluronic acid. Potato protein (patent document 10), persimmon leaf extract or hawthorn fruit extract (patent document 11), rice bran solvent extract (patent document 12), iridaceae crocus saffron extract (patent document 13), cucurbitaceae Plant seed extracts (Patent Document 14) and the like are known, and some of these have been tried to be applied to quasi-drugs and cosmetics as collagen or hyaluronic acid production promoters. However, an extracellular matrix production promoter that exhibits a satisfactory effect has not been obtained.

  The report regarding the preparation method of the plant extract from a plant includes a mulberry leaf extract (Patent Document 15), a saw palmetto extract (Patent Document 16), a processed sweet potato stem and leaves (Patent Document 17), and the like. A method for producing water-soluble polysaccharides for soybean-related extracts (Patent Document 18), a method for producing isoflavone compounds (Patent Document 19), and a method for producing γ-aminobutyric acid for wheat-related extracts (Patent Document 20) Etc. have been reported. However, a method for preparing a plant extract containing an effective ingredient by separating a liquid fraction after treating soybean-related or wheat-related materials under acidic conditions has not been obtained.

JP-A-62-84024 Japanese Patent Laid-Open No. 63-188867 Japanese Patent Laid-Open No. 03-141299 Japanese Patent Laid-Open No. 10-45615 Japanese Patent Laid-Open No. 10-36279 JP 2004-75632 A JP 2005-89375 A JP 2000-191498 A JP 2002-29980 A Japanese Patent Laid-Open No. 2005-263689 JP 2006-160629 A JP 2005-272433 A JP-A-2005-206510 JP 2006-273815 A JP 2004-217532 A JP 2005-336165 A JP 2005-278596 A Japanese Patent No. 2891081 JP 11-263786 A Japanese Patent No. 2813771 FRAGRANCE JOURNAL, 12, 51-55, 2004 FRAGRANCE JOURNAL, 26 (1), 45-50, 1998

  Giving cell activation and anti-aging to mammals, especially humans, is an extremely important issue, and various activators and anti-aging agents derived from animals and plants have been found. Sufficient and stable effects are not obtained to the extent that they can be used, and new cell activators and anti-aging agents are currently being searched.

  Accordingly, the first object of the present invention is to provide a cell activator or anti-aging agent that is derived from plants and has an excellent activation or anti-aging effect on cells, and has sufficient safety to withstand long-term use. And providing a method for use in cosmetics and quasi-drugs (external preparations for skin, bath preparations, hair-restoring agents, etc.), foods and drinks, and pharmaceuticals using these as active ingredients.

  The second object of the present invention is to provide a method for preparing a plant extract containing an effective component in a high yield from a plant material with high safety, and to provide the plant extract.

As a result of diligent efforts to achieve the above object, the present inventors have found a method for efficiently preparing a large amount of a plant extract that can be used for health, cosmetics, food, and pharmaceutical applications from a plant. It came to be completed. Furthermore, the inventors have found that plant extracts derived from wheat or soybean in particular are effective for cell activation, anti-aging, and extracellular matrix production, and have completed the present invention. That is, the present invention has the following configuration.
Item 1. It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. Anti-aging agent.
Item 2. It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. Cell activating agent.
Item 3. It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. And an extracellular matrix production promoter.
Item 4. It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. A collagen production promoter.
Item 5. It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. Hyaluronic acid production promoter.
Item 6. A plant extract obtained from at least one selected from the group consisting of soybean seeds, soybean germs, soybean embryos, soybean germs, wheat seeds, wheat germs, wheat germs, wheat germs, soy milk, and okara is used as animal skin. The anti-aging method characterized by including the process made to contact.
Item 7. A plant extract obtained from at least one selected from the group consisting of soybean seeds, soybean germs, soybean embryos, soybean germs, wheat seeds, wheat germs, wheat germs, wheat germs, soy milk, and okara is used as animal skin. The cell activation method characterized by including the process made to contact.
Item 8. The anti-aging agent according to Item 1, the cell activator according to Item 2, the extracellular matrix production promoter according to Item 3, the collagen production promoter according to Item 4, or the hyaluronic acid production promotion according to Item 5. Cosmetics characterized by containing an agent.
Item 9. The anti-aging agent according to Item 1, the cell activator according to Item 2, the extracellular matrix production promoter according to Item 3, the collagen production promoter according to Item 4, or the hyaluronic acid production promotion according to Item 5. A quasi-drug containing an agent.
Item 10. The anti-aging agent according to Item 1, the cell activator according to Item 2, the extracellular matrix production promoter according to Item 3, the collagen production promoter according to Item 4, or the hyaluronic acid production promotion according to Item 5. Foods and drinks characterized by containing an agent.
Item 11. The anti-aging agent according to Item 1, the cell activator according to Item 2, the extracellular matrix production promoter according to Item 3, the collagen production promoter according to Item 4, or the hyaluronic acid production promotion according to Item 5. Pharmaceuticals characterized by containing an agent.
Item 12. Contact the animal skin with a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara A method of promoting extracellular matrix production, comprising the step of:
Item 13. Contact the animal skin with a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara A method for promoting collagen production, comprising the step of:
Item 14. Contact the animal skin with a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara And a hyaluronic acid production promoting method comprising the step of:
Item 15. (1) A method for preparing a plant extract, comprising a step of treating a plant and / or plant extract under acidic conditions, and (2) a step of separating a liquid fraction.
Item 16. Item 16. The method for preparing a plant extract according to Item 15, wherein an acid solution containing a mineral acid and / or an organic acid is added and treated under acidic conditions.
Item 17. Item 16. The method for preparing a plant extract according to Item 15, wherein an acid solution containing a mineral acid and / or an organic acid is added so as to have a pH of 2 or less and the mixture is treated under acidic conditions.
Item 18. Mineral acid and / or organic acid is hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, perchloric acid, citric acid, lactic acid, propionic acid, butyric acid, succinic acid, malonic acid, succinic acid, fumaric acid, maleic acid Item 18. The method for preparing a plant extract according to Item 16 or 17, which is at least one acid selected from the group consisting of malic acid, tartaric acid, benzoic acid, sulfosalicylic acid and formic acid.
Item 19. Item 19. The method for preparing a plant extract according to any one of Items 15 to 18, wherein the acid solution added so as to be in an acidic condition is hydrochloric acid and / or perchloric acid and / or sulfuric acid.
Item 20. Item 20. The plant extract according to any one of Items 15 to 19, wherein a polyphenol adsorbent is added and a liquid fraction is separated simultaneously with or after the plant and / or plant extract is treated under acidic conditions. Preparation method.
Item 21. Item 15 is characterized in that the plant and / or plant extract is at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, wheat seed, wheat germ, wheat embryo, soy milk, and okara. The preparation method of the plant extract in any one of -20.
Item 22. Item 22. The method for preparing a plant extract according to any one of Items 15 to 21, wherein the step of separating the liquid fraction is centrifugation and / or filtration.
Item 23. Item 15. The plant extract recovered by the preparation method according to any one of Items 15 to 22, further comprising at least one selected from the group consisting of an ion exchange method, a gel filtration method, a membrane fractionation method, and an electrodialysis method. A method for preparing a plant extract, which is purified by treatment.
Item 24. Item 24. A cell activator or anti-aging agent comprising a plant extract recovered by the preparation method according to any one of Items 15 to 23.
Item 25. Item 24. An extracellular matrix production promoter containing a plant extract recovered by the preparation method according to any one of Items 15 to 23.
Item 26. Item 24. A collagen production promoter comprising a plant extract recovered by the preparation method according to any one of Items 15 to 23.
Item 27. Item 24. A hyaluronic acid production promoter containing a plant extract recovered by the preparation method according to any one of Items 15 to 23.

  According to the present invention, it has become possible to provide a method for preparing a large amount of a plant extract containing an active ingredient in a high yield from a plant material. Furthermore, it can be expected to have plant-derived safety-enhanced cell activation, anti-aging, and extracellular matrix production promoting effects, and it is a cosmetic / quasi-drug (skin) with a plant extract derived from wheat or soybean as an active ingredient. External preparations, bath preparations, hair restorers, etc.), foods and drinks, and pharmaceuticals can be provided.

Cell activation and anti-aging activity by soybean germ extract using human skin fibroblasts Cell activation and anti-aging activity by wheat germ extract using human skin fibroblasts Cell activation and anti-aging activity by soybean germ extract using human hair papilla cells Cell activation and anti-aging activity by wheat germ extract using human hair papilla cells Collagen production promoting activity by soybean germ extract and wheat germ extract Collagen production promoting activity by soybean germ extract and wheat germ extract (3 days culture) Collagen production promoting activity by soybean germ extract and wheat germ extract (6 days culture) Hyaluronic acid production promoting activity by soybean germ extract and wheat germ extract (3 days culture) Hyaluronic acid production promoting activity by soybean germ extract and wheat germ extract (6 days culture) Collagen production promoting activity by soybean germ extract (6 days culture) Collagen production promoting activity by wheat germ extract (6 days culture)

  In the present invention, “cell activation” and “anti-aging” are to reduce a decrease in cell function or cell activity by actively increasing or maintaining the cell function or cell activity of an animal. For example, skin cells are elastic by preventing and improving skin wrinkles, sagging, hardening, etc. by reducing the decrease in skin cell function associated with the accumulation of structural changes in the sublamellar membrane due to aging and photoaging. It refers to maintaining a youthful and healthy skin condition. In hair papilla or hair matrix cells, it refers to the suppression of hair loss by maintaining the hair cycle by reducing the decrease in function of hair milk or hair matrix cells due to aging, stress, and hormone balance.

  In the present invention, “contact with animal skin” means that plant extracts can be applied to humans, domestic animals, etc. in all dosage forms (ampoules, capsules, powders, granules, pills, tablets, Solid, liquid, gel, foam, emulsion, cream, ointment, sheet, mousse, etc.) for external application, external treatment, oral administration, and the like. For example, a plant extract can be blended in cosmetics, quasi drugs, foods and drinks, pharmaceuticals, feeds, and the like and brought into contact with animal skin.

  “Promoting production of extracellular matrix” is to promote production of extracellular matrix such as elastin, collagen, hyaluronic acid, etc., which supports the extracellular skin structure. A substance that promotes extracellular matrix production in this way is called “extracellular matrix production promoter”. By promoting extracellular matrix production, “anti-aging” is realized. The “extracellular matrix production promoter” includes “elastin production promoter”, “collagen production promoter” and “hyaluronic acid production promoter”. In particular, the “collagen production promoter” refers to a substance that promotes collagen production among the “extracellular matrix production promoters”. The “hyaluronic acid production promoter” refers to a substance that promotes the production of hyaluronic acid among the “extracellular matrix production promoters”.

  In the present invention, the “plant and / or plant extract” refers to various plants, plant extracts, and plant processed products, and is not particularly limited. The plant from which the plant extract is obtained is not particularly limited. For example, dicotyledonous plants, monocotyledonous plants, herbaceous plants, woody plants, cucurbitaceae plants, solanaceous plants, gramineous plants, cruciferous plants , Leguminous plant, mallow family, asteraceae plant, red crustacean plant, legume plant, plant extract, plant extract, and the like. For example, sweet potato, tomato, cucumber, pumpkin, melon, watermelon, tobacco, Arabidopsis, pepper, eggplant, bean, taro, spinach, carrot, strawberry, potato, rice, corn, alfalfa, wheat, barley, soybean, rapeseed, sorghum, Eucalyptus, poplar, kenaf, Tochu, sugar cane, sugar beet, cassava, sago palm, red pepper, lily, orchid, carnation, rose, chrysanthemum, petunia, torenia, snapdragon, cyclamen, gypsophila, geranium, sunflower, shiba, cotton, enoki mushroom , Matsutake, shiitake mushrooms, ginseng, agaricus, turmeric, ginseng, citrus, green tea, tea, oolong tea, banana, kiwi, natto, soy milk, soybean extract, wheat extract, Bud extract, embryo extract, fruit juice, Ocala, rice germ, wheat germ, barley germ, soybean germ, corn germ, milo germ, such as sunflower germ, and the like.

  Preferred are monocotyledonous plants and dicotyledonous plants, more preferred are grasses and legumes, and particularly preferred are corn, mushrooms, soybean, wheat, natto, soy milk, okara, wheat germ, soybean germ. Corn germ, soybean extract, wheat extract, germ extract, embryo extract, and more preferably soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat embryo, wheat germ, soy milk, and okara.

  The plant tissue for obtaining the plant extract (collecting the plant extract) is not particularly limited. Preferably, the seed is in the form of seed or growth process. Plants in the process of growing can be obtained from whole or partial tissues. The sites that can be obtained are not particularly limited, but include whole trees, flowers, buds, ovary, fruits, leaves, cotyledons, stems, buds, roots, seeds, dried seeds, embryos, embryos, roots, and the like. Preferred are fruits, leaves, stems, buds, seeds, dried seeds, embryos, embryos, and particularly preferred are seeds, dried seeds, germs, embryos, and the like.

  In the present invention, the “plant extract” refers to a (natural) plant extract obtained from plants and / or plant processed products or processed products thereof. The plant from which the plant extract is obtained may be a processed plant product. The processing method (preparation method of plant extract) is a method of impregnating a plant with water, an organic solvent, a mixture of water and an organic solvent, etc. at a low temperature, room temperature, and under a heating condition, a distillation method, a pressing method, Extracts are collected by ultrasonic method, supercritical fluid method, subcritical fluid method, etc. Furthermore, the processed material etc. which fermented the extract collect | recovered from the plant and the plant are included. For example, plant extracts, soy milk, okara, wheat flour, fermented extract, natto and the like can be mentioned.

  The plant from which the plant extract is obtained is particularly preferably selected from soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara.

  One of the important disclosures in the present invention is that an acid solution is added to a plant and / or plant extract (especially soybean or wheat-related material) so as to be in an acidic condition, and a liquid fraction is separated in a purification process. There is to do. The present inventors have found that a plant extract containing a large amount of active ingredients can be highly stably purified by this process.

  In the present invention, “under acidic conditions” refers to conditions under which the pH is 6 or less. By effecting the pH under acidic conditions during purification, an efficient and stable recovery of plant extract from plant tissue can be obtained. This effect is uniformly obtained as long as the pH is 6 or less, but is preferably 4 or less, particularly preferably 2 or less. The lower limit is not particularly limited because it may be the pH of the stock solution of the acid solution to be used, but is preferably pH 0-2.

  Acid solutions to be added under acidic conditions include mineral acids such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, propionic acid, butyric acid, succinic acid, malonic acid, succinic acid, fumaric acid, maleic acid, malic acid, lactic acid Organic acids such as tartaric acid, citric acid, benzoic acid and acidic water, but 0.01N to 6N hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, sulfosalicylic acid, formic acid, citric acid, lactic acid, Inorganic acids such as 0.1 to 10% perchloric acid and organic acids. Preferred are 0.0625 to 1N hydrochloric acid, 0.25 to 5% perchloric acid, 0.1 to 1N sulfuric acid, and the like.

  By obtaining a plant extract under acidic conditions (acid aqueous solution), it contains active and natural ingredients that are solubilized only under acidic conditions compared to plant extracts recovered with organic solvents such as ethanol and methanol. In addition, the components contained in the plant extract are limited compared to the organic solvent. Moreover, the stability of an active ingredient and a natural ingredient increases by extracting on acidic conditions. In addition, since the recovered plant extract is an aqueous solution, a high merit is expected in terms of safety as compared with an organic solvent.

  Another important disclosure in the present invention is to add a “polyphenol adsorbent” in the purification process of the plant extract. It has been found that the addition of a polyphenol adsorbent is important for removing substances other than active ingredients, removing decomposed substances of active ingredients, and removing substances that affect the stability of plant extracts.

  In the present invention, the “polyphenol adsorbent” is not particularly limited as long as it is a substance capable of adsorbing polyphenols, but PVPP (polyvinyl polypyrrolidone), PVP (polyvinyl pyrrolidone), PEG (polyethylene glycol) and the like are preferably used. The Particularly preferred is PVPP (polyvinyl polypyrrolidone). These polyphenol adsorbents may use commercially available materials. For example, Polyclar (registered trademark), POLYCLAR (registered trademark) (manufactured by ISP), Dowex (registered trademark) -1, Divergan (registered trademark) (manufactured by BSAF), PVP-40, or the like may be used. By adding a polyphenol adsorbent after adding an acid solution to a plant and / or plant extract, it can be expected that the recovered amount, yield, and purity of the plant extract will increase.

  The amount of polyphenol adsorbent added is preferably 0.1 to 30% (w / v), more preferably 0.5 to 20% (w / v), still more preferably 1 to 10% (w / v). It is.

  By crushing, crushing, and mixing the plant and / or plant extract after adding the acid solution to the acidic condition or adding the acid solution to the acidic condition and then adding the polyphenol adsorbent The amount of recovered plant extract can be increased. Particularly in the case of plant tissue, it is desirable to damage the cell wall because it has a cell wall. In the case of a plant extract or plant extract, it does not need to be crushed or crushed so as to damage the cell wall because it does not contain the cell wall. As a method for performing crushing or crushing, for example, a mixer, a blender, a homogenizer, a mortar, an ultrasonic crusher, or the like can be used.

  After the active ingredient contained in the plant and / or plant extract is sufficiently extracted into the acid solution (liquid fraction), the liquid fraction is separated from the residue and precipitate by centrifugation or filtration. The recovered liquid fraction contains a large amount of active ingredients, and the “plant extract” in the present invention is obtained.

If necessary, the plant extract may be subjected to a desalting treatment or a purification treatment by an ion exchange method, a membrane fractionation method, a gel filtration method, or an electrodialysis method, and by carrying out at least one of these methods. A more highly pure plant extract can be obtained. For example, as an ion exchange method, a plant extract is passed through a column filled with an ion exchange resin, and an active ingredient and impurities such as amino acids, peptides, proteins, and saccharides are separated. As the ion exchange resin to be used, the ion exchange group may be a sulfonic acid group, sulfopropyl group, phosphoric acid group, carboxylmethyl group, aminoethyl group, diethylamino group, quaternary aminoethyl group, quaternary ammonium group, etc. Either a cation exchange resin or an anion exchange resin can be used. When a cation exchange resin is used, the active ingredient is adsorbed on the cation exchange resin, so after separating the non-adsorbed substance sufficiently, it is effective in an acidic solution such as sulfuric acid or hydrochloric acid, or a salt solution such as sodium chloride. Elute the components. When an anion exchange resin is used, since the active ingredient is not adsorbed on the anion exchange resin, the non-adsorbed fraction containing the active ingredient is collected. For example, as a membrane fractionation method, cellulose, cellulose acetate, polysulfone, polyamide, polyacrylonitrile, polytetrafluoroethylene, polyester, polypropylene and the like have a fractional molecular weight in the range of 1000 to 100,000. The UF of the plant extract is performed using an ultrafiltration (UF) membrane and the permeate containing the active ingredient is recovered. Further, NF of the active ingredient solution is performed using a nanofiltration (NF) membrane having a salt rejection rate of 30 to 80% for desalting. For example, in the gel filtration method, a plant extract is neutralized and passed through a column packed with a gel filtration carrier to recover an active ingredient by molecular weight fractionation. The gel filtration carrier used is a dextran-based, acrylamide-based, agarose-based, cellulose-based, polyvinyl-based, glass-based, polystyrene-based, or the like with a molecular weight cut-off in the range of 100 to 100,000. For example, in electrodialysis, electrodialysis is performed by alternately supplying a plant extract and saline between each membrane partitioned by a cation exchange membrane and an anion exchange membrane. The electrodialysis conditions include an initial current density of 0.5 to 15 A / dm ² and a voltage of 0.1 to 1.5 V / tank.

  Plant extracts may be used as cell activators and anti-aging agents as they are, but should be used in cosmetics and quasi-drugs (external preparations for skin, bath preparations, hair restorers, etc.), foods and pharmaceuticals. Is preferred. The concentration at which the plant extract is blended is determined by the degree of absorption, the degree of action, the product form, the frequency of use, etc., and is not particularly limited, but is usually 0.0001 to 100%, preferably 0.005 to 75%, more preferably 0.001 to 50%.

  Since the “plant extract” of the present invention is derived from a natural plant, it is extremely safer than chemically synthesized products (there is no contamination of substrates, catalysts, unnecessary reactants, etc. used for chemical synthesis). Among plants, plant extracts derived from edible plants that have a large amount of net food per person per person per year are highly safe. For example, the net food per person per year per person per year in 2003 is 61.9 kg. 32.6 kg of wheat, 4.6 kg of sweet potato, 15.2 kg of potato, 6.7 kg of soybean, 5.5 kg of mandarin orange, and 8.2 kg of apple. In particular, chemically synthesized products cannot be used in functional foods, and natural products, particularly plants, are desired in cosmetics. Moreover, various natural components are contained in the plant-derived extract, and higher effects are expected when the components act synergistically. In terms of cost, plant-derived materials that are cultivated or marketed may be used. In particular, wheat and soybean seeds are sold at low prices among plant materials, and a large amount of materials can be secured. The production cost of the extract is lower than that of chemically synthesized products. Therefore, the usefulness of the cell activator or anti-aging agent characterized by using the plant extract of the present invention as an active ingredient is very high.

  The cell activator and anti-aging agent of the present invention are components and additives used for cosmetics, quasi-drugs, foods and drinks, pharmaceuticals, etc., as long as they do not impair the effects of the present invention. Can be used in combination.

  For example, as fats and oils, avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, beef fat, beef tallow fatty acid, cucumber nut oil, Safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, cacao butter, palm oil, palm kernel oil, molasses, palm oil Beef tallow, pork tallow, hydrogenated oil, hydrogenated castor oil, and the like.

  Examples of waxes include beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, and the like.

  Examples of the mineral oil include liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax, polyethylene powder, squalene, squalane and pristane.

  Examples of fatty acids include lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid and other natural fatty acids, isononanoic acid, caproic acid, 2- Synthetic fatty acids such as ethyl butanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid are exemplified.

  Alcohols include natural alcohols such as ethanol, isopyropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol, oxidation Ethylene, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene glycol, 1,3-butylene glycol, Glycerin, batyl Alcohol, pentaerythritol, sorbitol, mannitol, glucose, polyhydric alcohols such as sucrose and the like.

  Esters include isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate, Examples include diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

  Examples of the metal soap include aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, and zinc undecylate.

  Gum and water-soluble polymer compounds include gum arabic, benzoin gum, danmar gum, guaiac oil, Irish moss, Karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, carboxyalkyl Chitin, chitosan, hydroxyalkylchitin, low molecular chitosan, chitosan salt, sulfated chitin, phosphorylated chitin, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate, heparin, ethylcellulose, methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxy Sodium ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose, polyvinyl chloride Alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

  Surfactants include anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant (carboxylic acid) Type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester type non-type) Ionic surfactants, ester-type nonionic surfactants, block polymer-type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants, derivatives of protein hydrolysates, Examples thereof include polymer surfactants, surfactants containing titanium and silicon, and fluorocarbon surfactants.

  As vitamins, retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group, thiamine hydrochloride, thiamine sulfate (vitamin B1), Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group, ascorbic acid and its derivatives, vitamin D group , Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group, tocopherol and its derivatives, ubiquinones, vitamin K group, phytonadione (vitamin K1), menaquinone (bi Tamine K2), menadione (vitamin K3), menadiol (vitamin K4) and the like.

  As amino acids, valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine Ornithine, histidine and the like, and sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

  Whitening agents include ascorbic acid or derivatives thereof, sulfur, placental hydrolysate, ellagic acid or derivatives thereof, kojic acid or derivatives thereof, glucosamine or derivatives thereof, arbutin or derivatives thereof, hydroxycinnamic acid or derivatives thereof, glutathione, arnica Extract, Ogon extract, Sakuhakuhi extract, Psycho extract, Bowfu extract, Manntake mycelium culture or its extract, Linden extract, Peach leaf extract, Ages extract, Kujin extract, Gyuyu extract, Toki extract, Yokuinin extract, Oyster leaf extract, Daio extract , Button pipi extract, clam extract, horse chestnut extract, hypericum extract, oil-soluble licorice extract and the like.

  As humectants, hyaluronic acid, polyglutamic acid, serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, glyceroglycolipid, sphingophospholipid , Glycosphingolipid, linoleic acid or esters thereof, eicosapentaenoic acid or esters thereof, pectin, bifidobacteria fermentation product, lactic acid fermentation product, yeast extract, litchi mycelium culture or extract thereof, wheat germ oil, avocado Oil, rice germ oil, jojoba oil, soybean phospholipid, γ-oryzanol, bellows oyster extract, yokuinin extract, siamese extract, typhoid extract, diatomaceous earth extract, beetle aloe extract, burdock extract, mannen wax extract Arnica extract, such as wheat bran, and the like.

  As hair restorer, pentadecanoic acid glyceride, coleus extract, gentian extract, pine coconut extract, royal jelly extract, kumazasa extract, t-flavanone, 6-benzylaminopurine, assembly extract, carpronium chloride, minoxidil, finasteride, adenosine, nicotinamide, Examples include mulberry root extract, diau extract and 5-aminolevulinic acid.

  As an extract or extract of animal or plant, herbal medicine, Asenyaku (Asen Yaku), Ashitaba, Acerola, Altea, Arnica, Avocado, Achacha (Amacha), Aloe, Aloe Vera, Nettle, Ginkgo (Ginkgo leaf, Ginkgo), Fennel ( Ayaka), Turmeric (Usukin), Usubasaicin (Spicy), Japanese apricot (Plum), Vulture, Uwaurushi, Neubara (Natural Fruit), Hikikoshi (Elongation Grass), Ogi (Twilight), Koganebana (Ougon), Yamazakura (Cherry Blossom) ), Yellowfin, yellow orchid, ginseng, carrot, helicopter, licorice, Dutch pepper, orange, yellow-bellied (dianthus), crabs (summer hay), tsurudukudami (what heads), Enju ), Mugwort (guy leaves), scallops (莪 朮), kudu (katsune), valerian (yoshikusa) ), Chamomile, yellow-crowned cucumber (Gurone), Chinese mugwort (licorice), licorice (licorice), Japanese dandelion (Japanese winter flower, Japanese winter leaf), raspberry, kiwi fruit, Japanese cypress (flowerflower), chrysanthemum (Chrysanthemum flower) (Fruit), citrus fruit (fruit), tachibana (tachibana bark), cucumber, udo or shishiudo (clam alive, solitary), apricot (apricot jelly), wolfberry (earthbone bark, coconut, coconut leaf), Clara (bitter) ), Camphor, cinnamon, grapefruit fruit, Nikkei (Kaikin), Kaigai (Kei-Gai), Ebisu gussa (Keiko), Maruba morning glory or morning glory (ken cow), safflower (red flower), gobishi (penta), comfrey, copaiba, gardenia (Yamako), Gentiana, Honoki (Koh-pak), Hinata Taikozuchi (cow knee), Goshuyu (Kurean), Burdock, Korean ginger (Gomi) Child), Rice, Rice bran, Wheat, Mishima Saiko (Saiko), Saffron, Savonso, Hawthorn (Yamazako), Salamander (Samurai), Salvia, Sanchinin ginseng (Thirty-seven ginseng), Shiitake mushroom (Shibai), Giou ( Ground yellow), shikunshi (messenger), purple (purple), perilla (purple leaves, shiso), oysters (pepper), peonies (glaze), psyllium (carrots, carrots), ginger (ginger), ginger (ginger)菖蒲), hornworm (Sadako), Shimosukeso, white birch, honeysuckle (gold and silver flowers, Shinobu), Ivy, Achillea millefolium, elderberry, red bean, elderberry (bone graft), mallow, senkyu (river cucumber), Assembly (this medicine), mulberry (mulberry white skin, mulberry leaf), jujube (large coral), soybean, taranki, chikutsujinjin (bamboo ginseng), Hanasuge (knowledge) ), Warmoko (land), Dokudami (10 medicines), Fuyumushinatsusatake (Cycium Cordyceps), red pepper, physalis (Torone), Tachikakuso, Ryokucha (green tea), kocha (tea), clove (clove), Eunshu mikan (Chenhide), camellia, camellia, capsicum (banban), Touki (toll), red snapper, daidai (orange peel), walnut mushroom (gem), corn (south fur), eucommia (Tochu, Tochu), tomato , Nanten (Southern fruit), garlic (large sun), barley (malt), hakusen (white birch), janohi (barley winter), parsley, batata, mint (light load), hamamelis, rose, loquat leaves (bamboo leaves) ), Matsuhodo, Grape or its leaves, Loofah, Bodaiju, Button (peony skin), Hops, Maikai (My buds), Pine leaves, Marronnier , Mannenrou, Mukuroji, Melissa, Merirot, Bokeh (Kiso), Palms, Peach (Momojin, Momoha), Giant oysters (Ishiboshi), Areca (Mellow wax), Mejijiki (Masuhagi), Cornflower, Yukinoshita (Tiger ear grass), Prunus (Plum), Yachabushi (Yakuin), pearl barley (Yokuinin), mugwort, lavender, apple fruit, garlic mushroom (ganoderma), lemon fruit, forsythia (rendoku), forsythia, genokosho (old lady), ashridokoro (roth) Root), chicken crest, cattle / human placenta extract, pig / cow stomach, duodenum, or intestinal extract or its degradation product, water-soluble collagen, water-soluble collagen derivative, collagen hydrolyzate, elastin, elastin water Degradation product, water-soluble elastin derivative, silk protein, silk protein degradation product, bovine blood Such as protein decomposition products and the like.

  Examples of microorganism culture metabolites include yeast extract, zinc-containing yeast extract, germanium-containing yeast extract, selenium-containing yeast extract, magnesium-containing yeast extract, rice fermentation extract, Euglena extract, and lactic acid fermented milk powder.

  Examples of the α-hydroxy acid include glycolic acid, citric acid, malic acid, tartaric acid, and lactic acid.

  Inorganic pigments include silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, Examples include Bengala, black iron oxide, Gunjo, chromium oxide, chromium hydroxide, carbon black, and calamine.

  Examples of ultraviolet absorbers include p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, Examples include pyrone derivatives, nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin B6 derivatives, oxybenzone, benzophenone, guaiazulene, shikonin, baicalin, baicalein, and berberine.

  Astringents include lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid, etc. Can be mentioned.

  Antioxidants include ascorbic acid and its salts, stearic acid ester, tocopherol and its ester derivatives, nordihydrogua cetelenic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), parahydroxyanisole, propyl gallate , Sesamol, sesamorin, gossypol, etc.

  Anti-inflammatory agents include ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, Examples thereof include glycyrrhetinic acid and a salt thereof.

  Examples of the disinfectant / disinfectant include acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

  For hair, selenium disulfide, alkylisoquinolinium bromide, zinc pyrithione, biphenamine, thianthol, castari tincture, ginger tincture, pepper tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, sodium bromate, thiol Examples include glycolic acid.

  Perfumes include natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, Iran essential oil, Iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil, cumin Essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil, large Perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petit gren essential oil, bay essential oil, vetiva essential oil, bergamot Oil, Peru balsam essential oil, Boadrose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary essential oil, Japanese mint essence essential oil, etc. Examples include synthetic fragrances.

  As dyes and colorants, red cabbage dye, red rice dye, red dye, anato dye, squid dye, turmeric dye, enju dye, krill dye, amber dye, caramel, gold, silver, gardenia dye, corn dye, onion dye , Tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin and the like.

  Examples of the sweetener include sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin and the like.

  Examples of the nutrient fortifier include shell calcined calcium, cyanocolabamine, yeast, wheat germ, soybean germ, egg yolk powder, hemicellulose, heme iron and the like.

  In addition, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, refreshing agents, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animal / plant polysaccharides and their Degradation product, animal / plant glycoprotein and its degradation product, blood flow promoter, anti-inflammatory agent / antiallergic agent, cell activator, keratolytic agent, wound treatment agent, foam enhancer, thickener, oral preparation, Deodorizing / deodorizing agents, bittering agents, seasonings, enzymes and the like.

  The dosage form of the present invention is arbitrary, ampoules, capsules, powders, granules, pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets It can be used in combination with quasi-drugs such as mousse, cosmetics for skin and hair, bath preparations, foods and drinks, and pharmaceuticals.

  Specific examples of cosmetics and quasi-drugs include basic cosmetics such as internal and external pharmaceutical preparations, lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers, skin cleansers, shampoos, etc. , Rinse, hair treatment, hair cream, pomade, hair spray, hairdressing agent, perm, hair art, hair dye, hair growth, hair restoration, etc., foundation, white powder, funny, lipstick, blusher, eye shadow, eye Makeup cosmetics such as liners, mascara, eyebrows, eyelashes, finishing cosmetics such as nail polishes, perfumes, bath preparations, other toothpastes, mouth fresheners, gargles, liquid odors and deodorants, hygiene Goods, sanitary cotton, wet tissue and the like.

  Examples of foods and beverages include soft drinks, carbonated drinks, nutrition drinks, fruit drinks, lactic acid drinks, ice cream, ice sherbet, shaved ice and other frozen desserts, buckwheat, udon, harsame, gyoza skin, shumai skin, chinese food Noodles such as noodles, instant noodles, candy, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bakery confectionery, crab, salmon, clams, tuna, sardines, shrimp, Seafood such as skipjack, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, salmon roe, tocobushi, fishery products such as kamaboko, ham, sausage, milk, processed milk, fermented milk, etc. Fats and oils for products, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc. Processed foods, sauces, sauces and other seasonings, curry, stew, oyakodon, rice bowls, miscellaneous cooking, Chinese rice bowls, bowls, tempura, eel rice bowls, hayashi rice, oden, mabodorf, beef bowl, meat sauce, egg soup, omelet rice, dumplings Retort pouch foods such as shumai, hamburger and meatballs, various forms of health and nutritional supplements, health functional foods, tablets, capsules, drinks, troches and the like.

  The plant extract, cell activator, and anti-aging agent of the present invention are preferably applied to humans, but should be applied to animals other than humans as long as each effect can be expected. You can also.

  EXAMPLES The present invention will be described more specifically with reference to the following examples. However, these are merely examples and do not limit the scope of the present invention.

Example 1 Preparation of Plant Extract Containing Cell Activation and Anti-Aging Components from Soybean Seeds 480 mL of 5% aqueous perchloric acid solution was added to 100 g of soybean seeds (variety 'Fukuyutaka') overnight at room temperature. I left it alone. Thereafter, 16 g of polyclar VT (manufactured by ISP) as a polyphenol adsorbent was added, and the soybean seeds were sufficiently crushed with a blender mixer, and then allowed to stand at room temperature for 30 minutes and extracted under acidic conditions. The crushed material is centrifuged at 2 ° C., 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (soybean seed extract). did. Further, the recovered plant extract was passed through a column filled with a cation exchange resin (AG 50W-X4, 200-400mesh, H + type, manufactured by Bio-Rad), and cell activation and anti-aging components were adsorbed on the resin. The column was washed by flowing 0.7N NaCl / 0.1M sodium phosphate solution (pH 8.0), water and 1N hydrochloric acid sequentially. After removing impurities, cell activation and anti-aging components were eluted with 6N hydrochloric acid and neutralized with 30% sodium hydroxide to obtain a purified plant extract (purified soybean seed extract). Plant extract (soybean seed extract) and purified plant extract (purified soybean seed extract) were desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations. .

  To 100 g of soybean seeds (variety 'Fukuyutaka'), 500 mL of 1N hydrochloric acid solution was added and left overnight at a low temperature (4 ° C.). Thereafter, 16 g of polyclar VT (manufactured by ISP) as a polyphenol adsorbent was added, and the soybean seeds were sufficiently crushed with a blender mixer, and then left under low temperature (4 ° C.) for 30 minutes for extraction under acidic conditions. The crushed material is centrifuged at 2 ° C., 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (soybean seed extract). did. The recovered plant extract (soybean seed extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  From the above results, a plant extract containing cell activating and anti-aging components that can be used industrially or practically from soybean seeds could be obtained. In particular, since it has been confirmed that a plant extract containing cell activation and anti-aging components can be recovered from a plant with 1N hydrochloric acid, a plant extract containing cell activation and anti-aging components excellent in safety is provided. Can do. If a plant extract containing cell activation and anti-aging components is collected with hydrochloric acid and neutralized with 30% sodium hydroxide, it becomes a neutralization reaction with very common ions, which is safer than trichloroacetic acid. it is conceivable that.

Example 2 Preparation of Plant Extract Containing Cell Activation and Anti-Aging Components from Soybean Buds Soybean seeds (variety 'Fukuyutaka') are absorbed and cultured at 26 ° C. for 2 days under light or dark conditions And germinated. About 50 g of bud parts of germinated soybeans cultured under light conditions or dark conditions were sampled. To 50 g of soybean shoots, 250 g of 5% aqueous perchloric acid solution and 8 g of polyclar VT (manufactured by ISP) as a polyphenol adsorbent were added, and the soybean shoots were sufficiently crushed with a homogenizer. Next, it was left for 30 minutes at room temperature and extracted under acidic conditions. The crushed material is centrifuged at 2 ° C., 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (soybean bud extract). did. The recovered plant extract (soybean bud extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  From the above result, the plant extract containing the cell activation and anti-aging component which can be utilized industrially or practically from soybean bud was able to be obtained.

Example 3 Preparation of Plant Extract Containing Cell Activation and Anti-Aging Components from Soybean Germ (Soybean Embryo) 500 mL of 5% perchloric acid aqueous solution was added to 100 g of soybean germ (manufactured by Foryou) at room temperature And left for 1 hour. Thereafter, 16 g of polyclar VT (manufactured by ISP), which is a polyphenol adsorbent, was added, and the soybean germ was sufficiently crushed with a blender mixer and then extracted under acidic conditions by leaving it at room temperature for 30 minutes. The crushed material is centrifuged at 2 ° C. and 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (soybean germ extract). did. Further, the recovered plant extract was passed through a column filled with a cation exchange resin (AG 50W-X4, 200-400mesh, H + type, manufactured by Bio-Rad), and cell activation and anti-aging components were adsorbed on the resin. The column was washed by flowing 0.7N NaCl / 0.1M sodium phosphate solution (pH 8.0), water and 1N hydrochloric acid sequentially. After removing impurities, cell activation and anti-aging components were eluted with 6N hydrochloric acid and neutralized with 30% sodium hydroxide to obtain this solution as a purified plant extract (purified soybean germ extract). Plant extract (soybean germ extract) and purified plant extract (purified soybean germ extract) were desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations. .

  5 kg of 1N hydrochloric acid solution was added to 1 kg of soybean germ (manufactured by Foryou) and left at room temperature for 1 hour. Thereafter, 80 g of polyphenol VT (ISP), which is a polyphenol adsorbent, was added, and the soybean germ was sufficiently crushed with a homogenizer and then left for 1 hour at room temperature to extract under acidic conditions. The crushed material is centrifuged at 2 ° C. and 22,000 × g for 30 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (soybean germ extract). did. The plant extract (soybean germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations.

  From the above results, it was revealed that a plant extract containing a high content of cell activation and anti-aging components can be obtained from soybean germ (soybean embryo). Since it was confirmed that a plant extract containing cell activation and anti-aging components can be recovered from the plant with 1N hydrochloric acid, a plant extract containing cell activation and anti-aging components excellent in safety can be provided. .

(Example 4) Cell activation from wheat germ (wheat embryo) and preparation of plant extract containing anti-aging components 500 g of 5% excess over 100 g wheat germ (cultured and roasted type, Nisshin Pharma) An aqueous chloric acid solution was added and the mixture was allowed to stand at room temperature for 1 hour. Thereafter, 16 g of polyclar VT (manufactured by ISP), which is a polyphenol adsorbent, was added, and the soybean germ was sufficiently crushed with a blender mixer and then extracted under acidic conditions by leaving it at room temperature for 30 minutes. The crushed material is centrifuged at 2 ° C. and 22,000 × g for 20 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (wheat germ extract). did. Further, the recovered plant extract was passed through a column filled with a cation exchange resin (AG 50W-X4, 200-400mesh, H + type, manufactured by Bio-Rad), and cell activation and anti-aging components were adsorbed on the resin. The column was washed by flowing 0.7N NaCl / 0.1M sodium phosphate solution (pH 8.0), water and 1N hydrochloric acid sequentially. After removing impurities, cell activation and anti-aging components were eluted with 6N hydrochloric acid and neutralized with 30% sodium hydroxide to obtain a purified plant extract (purified wheat germ extract). Plant extracts (wheat germ extract) and purified plant extracts (purified wheat germ extract) were desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations. .

  5 kg of 1N hydrochloric acid solution was added to 1 kg of wheat germ (cultured / roasted type, manufactured by Nisshin Pharma) and left at room temperature for 1 hour. Thereafter, 80 g of polyphenol VT (ISP), which is a polyphenol adsorbent, was added, and the wheat germ was sufficiently crushed with a homogenizer, and then left at room temperature for 1 hour for extraction under acidic conditions. The crushed material is centrifuged at 2 ° C. and 22,000 × g for 30 minutes to collect a liquid fraction, neutralized with a 30% sodium hydroxide solution, and this solution is used as a plant extract (wheat germ extract). did. The plant extract (wheat germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  From the above results, it was possible to obtain a plant extract containing cell activation and anti-aging components that can be used industrially or practically from wheat germ (wheat embryo). Since it was confirmed that a plant extract containing cell activation and anti-aging components can be recovered from the plant with 1N hydrochloric acid, a plant extract containing cell activation and anti-aging components excellent in safety can be provided. .

(Example 5) Preparation of a plant extract containing cell activation and anti-aging components from soy milk 100 mL of 5% perchloric acid aqueous solution was added to 100 mL of soy milk (variety 'Fukuyutaka'). Then, after sufficiently mixing with a mixer, the mixture was allowed to stand at room temperature for 30 minutes and extracted under acidic conditions. The mixture was centrifuged at 4 ° C., 27,700 × g for 20 minutes to collect a liquid fraction, which was neutralized with a 30% sodium hydroxide solution to obtain a plant extract (soy milk extract). The recovered plant extract (soy milk extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  100 mL of soy milk (variety 'Fukuyutaka') was added 2.5% (final concentration 0.25%), 5 mL (final concentration 0.5%), 10 mL (final concentration 1%), 20 mL (final concentration). Concentration 2%) and 40 mL (final concentration 4%) were added respectively. Then, after sufficiently mixing with a mixer, the mixture was allowed to stand at room temperature for 30 minutes and extracted under acidic conditions. The mixture was centrifuged at 2 ° C., 27,700 × g for 20 minutes to collect a liquid fraction, which was neutralized with a 30% sodium hydroxide solution to obtain a plant extract (soy milk extract). The liquid fraction was white at a perchloric acid concentration of 0.25%. It is considered that because the perchloric acid concentration was low, the soy protein was not sufficiently denatured due to a decrease in pH and was mixed into the liquid fraction. Slight white color was exhibited even at 0.5% and 1% perchloric acid concentrations. The recovered plant extract (soy milk extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations. It was confirmed that a plant extract containing cell activation and anti-aging components could be recovered even at a perchloric acid concentration of 0.5%, and an excellent preparation method was found in terms of safety.

  To 100 mL of soy milk (variety 'Fukuyutaka'), 6N hydrochloric acid was used to add final concentrations of 0.0625N, 0.125N, 0.25N, 0.5N, and 1N. Then, after sufficiently mixing with a mixer, the mixture was allowed to stand at room temperature for 30 minutes and extracted under acidic conditions. The mixture was centrifuged at 2 ° C., 27,700 × g for 20 minutes to collect a liquid fraction, which was neutralized with 30% sodium hydroxide to make this solution a plant extract (soy milk extract). The liquid fraction was slightly white at hydrochloric acid concentrations of 0.0625N, 0.125N, 0.25N, and 0.5N. It is considered that the soy protein was not sufficiently denatured due to a decrease in pH due to the low concentration of hydrochloric acid and mixed into the liquid fraction. The recovered plant extract (soy milk extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations. It was confirmed that plant extracts containing cell activation and anti-aging components could be recovered even with hydrochloric acid of 1N or less, and an excellent preparation method was found in terms of safety.

  From the above result, the plant extract containing the cell activation and anti-aging component which can be utilized industrially or practically from soybean milk was able to be obtained. When recovering a plant extract containing cell activation and anti-aging components from soy milk, there is no need for crushing or pulverization, and there are few preparation steps and great merit. Since soy milk is eaten and consumed in general, plant extracts containing cell activation and anti-aging components prepared from soy milk are extremely safe.

(Example 6) Preparation of a plant extract containing cell activation and anti-aging components from okara 5 L of 5% perchloric acid aqueous solution was added to 1 kg of okara and left at room temperature for 1 hour. Thereafter, the okara was sufficiently crushed with a homogenizer, and left under room temperature for 1 hour for extraction under acidic conditions. The crushed material was centrifuged at 2 ° C. and 22,000 × g for 30 minutes to collect a liquid fraction, which was neutralized with a 30% sodium hydroxide solution and used as a plant extract (Okara extract). . The plant extract (Okara extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  From the above results, a plant extract containing cell activating and anti-aging components that can be used industrially or practically from okara could be obtained. Okara was a residue after collecting soymilk and was thought to contain almost no cell activation and anti-aging components, but was confirmed to contain cell activation and anti-aging components. Since most of okara is discarded, a great industrial advantage can be expected by recovering plant extracts containing cell activation and anti-aging components using okara as a production material.

(Example 7) Evaluation using normal human skin fibroblasts with plant extract derived from soybean germ Plant extract derived from soybean germ was purified from the plant extract (soybean germ extract) described in Example 3. A plant extract (purified soybean germ extract) was used. The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 48-well microplate so as to be 2.0 × 10 ⁴ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. After culturing at 37 ° C. and a carbon dioxide concentration of 5 vol. Subsequently, the medium containing 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 100 μg / mL and cultured for 3 hours. Formazan generated by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in FIG. 1 as relative values with the cell activation effect in the control (water addition) as 100.

  As is apparent from FIG. 1, the medium supplemented with soybean germ extract and purified soybean germ extract has high cell activation / anti-aging effects on normal human skin fibroblasts. Admitted. In particular, soybean germ extract compared to blank (water) at 40 μg / mL, 400 μg / mL, 800 μg / mL, 2000 μg / mL concentrations, and purified soybean germ extract at 13 μg / mL, 26 μg / mL, 65 μg / mL concentrations. In both cases, significant cell activation / anti-aging effects of 20% or more were observed. From the above, it became clear that the plant extract containing these cell activation and anti-aging components has an excellent cell activation effect, and by applying these to the skin, fibroblasts associated with aging Reduced decline reduces decrease in collagen and hyaluronic acid turnover rate, exhibits excellent anti-aging effect, effectively improves skin wrinkles and sagging caused by aging, UV exposure, etc. can do.

(Example 8) Evaluation using normal human skin fibroblasts with plant extract derived from wheat germ Plant extract derived from wheat germ was purified with the plant extract (wheat germ extract) described in Example 4 A plant extract (purified wheat germ extract) was used. The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 48-well microplate so as to be 2.0 × 10 ⁴ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. After culturing at 37 ° C. and a carbon dioxide concentration of 5 vol. Subsequently, the medium containing 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 100 μg / mL and cultured for 3 hours. Formazan generated by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in FIG. 2 as relative values with the cell activation effect in the control (water addition) as 100.

  As is clear from FIG. 2, the medium supplemented with wheat germ extract and purified wheat germ extract has high cell activation / anti-aging effects on normal human skin fibroblasts. Admitted. In particular, wheat germ extract compared to blank (water) at 40 μg / mL, 400 μg / mL, 800 μg / mL, 2000 μg / mL concentrations, and purified wheat germ extract at 10 μg / mL, 20 μg / mL, 50 μg / mL concentrations. In both cases, significant cell activation / anti-aging effects of 20% or more were observed. From the above, it became clear that the plant extract containing these cell activation and anti-aging components has an excellent cell activation effect, and by applying these to the skin, fibroblasts associated with aging Reduced decline reduces decrease in collagen and hyaluronic acid turnover rate, exhibits excellent anti-aging effect, effectively improves skin wrinkles and sagging caused by aging, UV exposure, etc. can do.

(Example 9) Evaluation using human hair papilla cells with plant extract derived from soybean germ (1) Culture method of human hair papilla cells Human hair papilla cells (THPC-001) total kit (HDPC total kit: THPCK- 001, manufacturer: Cell Applications Inc. USA, import sales company: Toyobo Co., Ltd.), and cultured human hair papilla cells by a conventional method. Dispense PCGM medium for suspending thawed cells into 10 mL and 15 mL centrifuge tubes, and incubate with ice. That is, a vial containing frozen human hair dermal papilla cells (THPC-001) is rapidly thawed in a 37 ° C. constant temperature bath. About 1 mL of PCGM medium is gradually dropped into this vial to dilute DMSO, and the entire amount is transferred to a centrifuge tube containing PCGM medium and suspended. The suspended cells are centrifuged at 4 ° C. and 1000 rpm for 5 minutes in a cooling low centrifuge. Carefully aspirate the precipitated cells and resuspend in 1 mL PCGM medium. The whole amount is planted in a T-75 flask coated with a collagen solution, and placed in an incubator kept at 37 ° C. under a carbon dioxide concentration of 5 vol% under static humidity for stationary culture. After 1 day, the medium is changed. Thereafter, the culture medium is changed every other day and subcultured. The PCGM medium components were 250 mL of PCGM basal medium containing 1% FBS, 2.5 mL of bovine pituitary extract (BPE) 100-fold diluted solution, 2.5 mL of fetal calf serum (FCS) 100-fold diluted solution, 1.25 mL of a 200-fold diluted solution of insulin, transferrin, triiodothyronine solution (ITT) and 1.25 mL of a 200-fold diluted solution of siloprotein solution (Cyp) were used.

(2) Evaluation of dermal papilla cell activation activity Plant extract derived from soybean germ used the plant extract (soybean germ extract) and purified plant extract (purified soybean germ extract) described in Example 3 . The evaluation was performed according to the following procedure. Human hair papilla cells were seeded in a 48-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Subsequently, the medium containing 100 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced and cultured for 3 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in FIG. 3 as relative values with the cell activation effect in the blank with no sample taken as 100.

  As is clear from FIG. 3, the medium supplemented with soybean germ extract and purified soybean germ extract was found to have a high cell activation / anti-aging effect on human hair papilla cells. In particular, the soybean germ extract has a concentration of 400 μg / mL, 800 μg / mL, 2000 μg / mL, and the purified soybean germ extract has a concentration of 13 μg / mL, 26 μg / mL, and 65 μg / mL. A significant cell activation / anti-aging effect of 20% or more was observed. From the above, it has been clarified that the plant extract containing these cell activation and anti-aging components has an excellent cell activation effect, and when applied to the skin, it exhibits an extremely excellent anti-aging effect. In addition, it is possible to effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like. Furthermore, since excellent cell activation action has been confirmed in human hair papilla cells, it is possible to effectively improve aging, stress, hormone balance, hair formation inhibition caused by ultraviolet exposure, and the like.

(Example 10) Evaluation using human hair papilla cells with plant extract derived from wheat germ (1) Culture method of human hair papilla cells Human hair papilla cells (THPC-001) total kit (HDPC total kit: THPCK- 001, manufacturer: Cell Applications Inc. USA, import sales company: Toyobo Co., Ltd.), and cultured human hair papilla cells by a conventional method. Dispense PCGM medium for suspending thawed cells into 10 mL and 15 mL centrifuge tubes, and incubate with ice. That is, a vial containing frozen human hair dermal papilla cells (THPC-001) is rapidly thawed in a 37 ° C. constant temperature bath. About 1 mL of PCGM medium is gradually dropped into this vial to dilute DMSO, and the entire amount is transferred to a centrifuge tube containing PCGM medium and suspended. The suspended cells are centrifuged at 4 ° C. and 1000 rpm for 5 minutes in a cooling low centrifuge. Carefully aspirate the precipitated cells and resuspend in 1 mL PCGM medium. The whole amount is planted in a T-75 flask coated with a collagen solution, and placed in an incubator kept at 37 ° C. under a carbon dioxide concentration of 5 vol% under static humidity for stationary culture. After 1 day, the medium is changed. Thereafter, the culture medium is changed every other day and subcultured. The PCGM medium components were 250 mL of PCGM basal medium containing 1% FBS, 2.5 mL of bovine pituitary extract (BPE) 100-fold diluted solution, 2.5 mL of fetal calf serum (FCS) 100-fold diluted solution, 1.25 mL of a 200-fold diluted solution of insulin, transferrin, triiodothyronine solution (ITT) and 1.25 mL of a 200-fold diluted solution of siloprotein solution (Cyp) were used.

(2) Evaluation of dermal papilla cell activation action The plant extract derived from wheat germ used the plant extract (wheat germ extract) and the purified plant extract (purified wheat germ extract) described in Example 4 . The evaluation was performed according to the following procedure. Human hair papilla cells were seeded in a 48-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Subsequently, the medium containing 100 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced and cultured for 3 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in FIG. 4 as relative values with the cell activation effect in the blank with no sample as 100.

  As apparent from FIG. 4, it was confirmed that the medium supplemented with wheat germ extract and purified wheat germ extract has a high cell activation / anti-aging effect on human hair papilla cells. In particular, the wheat germ extract has a concentration of 800 μg / mL and 2000 μg · mL, and the purified soybean germ extract has a concentration of 10%, 20 μg / mL, and 50 μg / mL. Significant cell activation and anti-aging effects were observed. From the above, it has been clarified that the plant extract containing these cell activation and anti-aging components has an excellent cell activation effect, and when applied to the skin, it exhibits an extremely excellent anti-aging effect. In addition, it is possible to effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like. Furthermore, since excellent cell activation action has been confirmed in human hair papilla cells, it is possible to effectively improve aging, stress, hormone balance, hair formation inhibition caused by ultraviolet exposure, and the like.

(Example 11) Evaluation of collagen production ability by plant extracts derived from soybean germ and wheat germ The plant extract (soybean germ extract) described in Example 3 was used as the plant extract derived from soybean germ. . The plant extract derived from wheat germ was the plant extract described in Example 4 (wheat germ extract). The evaluation was performed according to the following procedure. Normal human skin fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 1.0 × 10 ⁵ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, after washing twice with PBS (−), the medium is replaced with a serum-free medium to which a sample of any concentration is added, and further cultured under the same conditions for 50 hours. did. From the culture supernatant, type I procollagen C-terminal peptide (Procollagen type I carboxyterminal propeptide: PIP) produced by human fibroblasts was measured with Procollagen type I C-peptide (PIP) EIA Kit (TaKaRa). Collagen production acceleration rate was determined by measuring standard products using the above ELISA kit, creating a calibration curve from the results, and determining the amount of collagen produced when the sample was added and the amount of collagen produced when no sample was added. Evaluation was performed by calculating the collagen production amount at the time of addition as 100%.

  As is clear from FIG. 5, it was confirmed that the collagen production activity (collagen production promoting action) of human skin was significantly increased in the medium supplemented with soybean germ extract or wheat germ extract. By applying a plant extract containing cell activation and anti-aging components to the skin, the decline in the rate of turnover of collagen and hyaluronic acid is reduced by reducing the decline of fibroblasts with aging. It exhibits an excellent anti-aging effect and can effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like.

(Example 12) Evaluation of collagen production ability by plant extracts derived from soybean germ and wheat germ The plant extract (soybean germ extract) described in Example 3 was used as the plant extract derived from soybean germ. . The plant extract derived from wheat germ was the plant extract described in Example 4 (wheat germ extract). The evaluation was performed according to the following procedure. Normal human skin fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 1.0 × 10 ⁵ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, after washing twice with PBS (−), the medium was replaced with a serum-free medium to which a sample of any concentration was added, and the conditions were the same for 3 days and 6 days. Incubated in From the culture supernatant, type I procollagen C-terminal peptide (Procollagen type I carboxyterminal propeptide: PIP) produced by human fibroblasts was measured with Procollagen type I C-peptide (PIP) EIA Kit (TaKaRa). Collagen production acceleration rate was determined by measuring standard products using the above ELISA kit, creating a calibration curve from the results, and determining the amount of collagen produced when the sample was added and the amount of collagen produced when no sample was added. Evaluation was performed by calculating the collagen production amount at the time of addition as 100%.

  As apparent from FIGS. 6 and 7, it was recognized that the collagen production activity (collagen production promoting action) of human skin was significantly increased in the medium supplemented with soybean germ extract or wheat germ extract. In all the extracts, compared with the blank (water), a significant collagen production promoting effect of 2 times or more was observed. By applying a plant extract derived from soybean germ or wheat germ to the skin, the decline of fibroblasts accompanying aging can be reduced, collagen production can be promoted, and the amount of collagen can be maintained. As a result, it is possible to effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like.

(Example 13) Evaluation of hyaluronic acid production ability by plant extracts derived from soybean germ and wheat germ The plant extract (soybean germ extract) described in Example 3 is used as the plant extract derived from soybean germ. It was. The plant extract derived from wheat germ was the plant extract described in Example 4 (wheat germ extract). The evaluation was performed according to the following procedure. Normal human skin fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 1.0 × 10 ⁵ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, after washing twice with PBS (−), the medium was replaced with a serum-free medium to which a sample of any concentration was added, and the conditions were the same for 3 days and 6 days. Incubated in Hyaluronic acid in the culture supernatant was measured. The cells on the plate were lysed with a cell lysate containing 0.5 Triton-X100, and after recovery, the amount of protein was measured (Bio Rad, protein assay kit), which was used as an indicator of the number of cells. For the measurement of hyaluronic acid, hyaluronic acid produced by normal human skin fibroblasts was measured by a sandwich method using hyaluronic acid binding protein (HABP) using a hyaluronic acid plate “Chugai” (Fujirebio). The production rate was defined as the amount of hyaluronic acid in the sample to which the plant extract was added when the amount of hyaluronic acid in the sample to which the plant extract was not added (control) was 100.

  As is clear from FIGS. 8 and 9, it was recognized that the hyaluronic acid production rate of human skin was significantly increased in the medium supplemented with soybean germ extract or wheat germ extract. In all of the extracts, a significant hyaluronic acid production promoting effect was observed as compared with the control. By applying the plant extract derived from soybean germ or wheat germ to the skin, the decline of fibroblasts accompanying aging can be reduced, the production of hyaluronic acid can be promoted, and the amount of hyaluronic acid can be maintained. As a result, it is possible to effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like.

(Example 14) Manufacture of essence The essence of the composition shown below was manufactured by the conventional method. As a control, a serum containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Sorbit 4.0
Dipropylene glycol 6.0
Polyethylene glycol 1500 5.0
POE (20) oleyl alcohol ether 0.5
Sucrose fatty acid ester 0.2
Methylcellulose 0.2
Purified soybean germ extract (concentration 13 mg / mL) 1.0
Total amount of purified water is 100

(Example 15) Manufacture of emulsion The emulsion of the composition shown below was manufactured by the conventional method. As a control, an emulsion containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Glyceryl ether 1.5
Sucrose fatty acid ester 1.5
Sorbitan monostearate 1.0
Squalane 7.5
Dipropylene glycol 5.0
Purified soybean germ extract (concentration 13 mg / mL) 1.0
Total amount of purified water is 100

(Example 16) Production of cream A cream having the composition shown below was produced by a conventional method. As a control, a cream containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Propylene glycol 6.0
Dibutyl phthalate 19.0
Stearic acid 5.0
Glycerol monostearate 5.0
Sorbitan monostearate 12.0
Polyethylene sorbitan monostearate 38.0
Sodium edetate 0.03
Purified soybean germ extract (concentration 13 mg / mL) 1.0
Total amount of purified water is 100

(Example 17) Sensory evaluation Sensory evaluation was performed using Examples 14-16. In addition, the comparative example which does not contain an embryo extract was also evaluated simultaneously. For sensory evaluation, 20 panelists aged 40 to 60 who are worried about aging symptoms such as wrinkles are taken as a group, and the examples and comparative examples are used twice a day for 3 months continuously. A questionnaire survey was conducted on the skin condition.

  Table 1 shows the number of evaluators in each item as a result of sensory evaluation. More than 80% of the panel responded that the elasticity of the skin was recovered and wrinkles were reduced, compared to the comparative example that did not contain the germ extract. It was shown that there is.

(Example 18) Manufacture of essence The essence of the composition shown below was manufactured by the conventional method. As a control, a serum containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Sorbit 4.0
Dipropylene glycol 6.0
Polyethylene glycol 1500 5.0
POE (20) oleyl alcohol ether 0.5
Sucrose fatty acid ester 0.2
Methylcellulose 0.2
Purified wheat germ extract (concentration 25 mg / mL) 1.0
Total amount of purified water is 100

(Example 19) Manufacture of emulsion The emulsion of the composition shown below was manufactured by the conventional method. As a control, an emulsion containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Glyceryl ether 1.5
Sucrose fatty acid ester 1.5
Sorbitan monostearate 1.0
Squalane 7.5
Dipropylene glycol 5.0
Purified wheat germ extract (concentration 25 mg / mL) 1.0
Total amount of purified water is 100

(Example 20) Production of cream A cream having the composition shown below was produced by a conventional method. As a control, a cream containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Propylene glycol 6.0
Dibutyl phthalate 19.0
Stearic acid 5.0
Glycerol monostearate 5.0
Sorbitan monostearate 12.0
Polyethylene sorbitan monostearate 38.0
Sodium edetate 0.03
Purified wheat germ extract (concentration 25 mg / mL) 1.0
Total amount of purified water is 100

(Example 21) Sensory evaluation Sensory evaluation was performed using Examples 18-20. In addition, the comparative example which does not contain an embryo extract was also evaluated simultaneously. For sensory evaluation, 20 panelists aged 40 to 60 who are worried about aging symptoms such as wrinkles are taken as a group, and the examples and comparative examples are used twice a day for 3 months continuously. A questionnaire survey was conducted on the skin condition.

  As a result of sensory evaluation, the number of evaluators in each item is shown in Table 2. More than 80% of the panel responded that the elasticity of the skin was recovered and wrinkles were reduced, compared to the comparative example that did not contain the germ extract. It was shown that there is.

(Example 22) Evaluation of collagen production ability by plant extract derived from soybean germ and wheat germ The extract derived from soybean germ was the plant extract (soy germ extract / purified soybean germ extract described in Example 3). Used). The extract derived from wheat germ used the plant extract described in Example 4 (wheat germ extract / purified wheat germ extract). The evaluation was performed according to the following procedure. Normal human skin fibroblasts were seeded in a 48-well microplate so that the number of normal human skin fibroblasts was 1.0 × 10 ⁵ per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing at 37 ° C. in a carbon dioxide concentration of 5 vol% for 24 hours, after washing twice with PBS (−), the medium was replaced with a serum-free medium to which a sample of any concentration was added, and cultured under the same conditions for 6 days. . From the culture supernatant, type I procollagen C-terminal peptide (Procollagen type I carboxyterminal propeptide: PIP) produced by human fibroblasts was measured with Procollagen type I C-peptide (PIP) EIA Kit (TaKaRa). The cells on the plate were lysed with a cell lysate containing 0.5% Triton-X100, and after recovery, the amount of protein was measured (Bio Rad, protein assay kit), which was used as an indicator of the number of cells. Collagen production acceleration rate is measured with the above-mentioned ELISA kit for the standard product, and a calibration curve is created from the results. From the calibration curve, the amount of collagen produced when the sample is added per protein (number of cells) and the collagen when no sample is added The production amount was determined, and the collagen production amount when no sample was added was calculated as 100% for evaluation.

  As is clear from FIG. 10 and FIG. 11, in the medium supplemented with soybean germ extract or wheat germ extract prepared under acidic conditions, it was recognized that the collagen production rate of human skin was significantly increased per cell. . In all the extracts, a significant collagen production-promoting effect was recognized as compared with the blank (water). Collagen production promotion effect was demonstrated by each extract per cell using protein amount as an index. Therefore, each extract extracts collagen production promotion (extracellular matrix production promotion) without cell proliferation (increase in cell number). Was found for the first time from this result. In other words, it was revealed that each extract has an action of increasing the amount of collagen produced per cell. From the above, by applying these extracts to the skin, the decline of fibroblasts accompanying aging can be reduced, collagen production can be promoted, and the amount of collagen can be maintained. As a result, it is possible to effectively improve skin wrinkles, sagging, dullness, etc. caused by aging, exposure to ultraviolet rays, and the like.

(Example 23) Component analysis of plant extracts derived from soybean germ and wheat germ The plant extract (soybean germ extract) described in Example 3 was used as the extract derived from soybean germ. The plant germ extract (wheat germ extract) described in Example 4 was used as the extract derived from wheat germ. As components, energy, moisture, protein, lipid, ash, carbohydrates, sodium and saccharides were analyzed. The analysis was conducted at the Mathis Food Safety Analysis Center. The analysis results of the components are shown in Table 3. Since the sample is an aqueous solution, it contains about 85% moisture, and the remaining about 15% solid components are carbohydrate (about 11-12%), protein (about 3%), ash (0.6-0.8). %) And lipid (about 0.1%). Carbohydrates accounted for most of the solid components, and about 70% of the carbohydrates were sugars. The remaining 30% appears to be nucleic acids, dietary fiber, etc. The breakdown of sugars is as follows: soybean germ extract is monosaccharide glucose and fructose (60%), disaccharide maltose (3%), trisaccharide raffinose, maltotriose (36%), wheat germ extract is glucose and Fructose (59%), lactose, sucrose (18%), raffinose, maltotriose (7.4%), and the rest were oligosaccharides with more than tetrasaccharide. From the above results, saccharides (especially monosaccharides / oligosaccharides and combinations thereof), which occupy most of the components of soybean germ extract and wheat germ extract, activate cells, promote anti-aging, promote extracellular matrix production, collagen It is considered to be one of the active ingredients for promoting production, hyaluronic acid production, and beautifying skin. In addition, soy germ extract and wheat germ extract are common in many monosaccharides and oligosaccharides because the components are extracted from soy, wheat seeds and germs under acidic conditions such as hydrochloric acid. I think it is a big point. However, this does not limit the present invention.

(Example 24) Manufacture of essence The essence of the composition shown below was manufactured by the conventional method. As a control, a serum containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Sorbit 4.0
Dipropylene glycol 6.0
Polyethylene glycol 1500 5.0
POE (20) oleyl alcohol ether 0.5
Sucrose fatty acid ester 0.2
Methylcellulose 0.2
Soybean germ extract (concentration 6mg / mL) 1.0
Total amount of purified water is 100

(Example 25) Production of emulsion An emulsion having the composition shown below was produced by a conventional method. As a control, an emulsion containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Glyceryl ether 1.5
Sucrose fatty acid ester 1.5
Sorbitan monostearate 1.0
Squalane 7.5
Dipropylene glycol 5.0
Soybean germ extract (concentration 6mg / mL) 1.0
Total amount of purified water is 100

(Example 26) Production of cream A cream having the following composition was produced by a conventional method. As a control, a cream containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Propylene glycol 6.0
Dibutyl phthalate 19.0
Stearic acid 5.0
Glycerol monostearate 5.0
Sorbitan monostearate 12.0
Polyethylene sorbitan monostearate 38.0
Sodium edetate 0.03
Soybean germ extract (concentration 6mg / mL) 1.0
Total amount of purified water is 100

(Example 27) Sensory evaluation Sensory evaluation was performed using Examples 24-26. In addition, the comparative example which does not contain an embryo extract was also evaluated simultaneously. For sensory evaluation, 20 panelists aged 40 to 60 who are worried about aging symptoms such as wrinkles are taken as a group, and each of Examples and Comparative Examples is used twice a day for 3 months continuously. A questionnaire survey was conducted on the skin condition.

  Table 4 shows the number of evaluators in each item as a result of the sensory evaluation. More than 80% of the panel responded that the elasticity of the skin was recovered and the wrinkles were reduced, compared to the comparative example that did not contain the germ extract. It was shown that there is.

(Example 28) Manufacture of essence The essence of the composition shown below was manufactured by the conventional method. As a control, a serum containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Sorbit 4.0
Dipropylene glycol 6.0
Polyethylene glycol 1500 5.0
POE (20) oleyl alcohol ether 0.5
Sucrose fatty acid ester 0.2
Methylcellulose 0.2
Wheat germ extract (concentration 6 mg / mL) 1.0
Total amount of purified water is 100

(Example 29) Manufacture of emulsion The emulsion of the composition shown below was manufactured by the conventional method. As a control, an emulsion containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Glyceryl ether 1.5
Sucrose fatty acid ester 1.5
Sorbitan monostearate 1.0
Squalane 7.5
Dipropylene glycol 5.0
Wheat germ extract (concentration 6 mg / mL) 1.0
Total amount of purified water is 100

(Example 30) Manufacture of cream The cream of the composition shown below was manufactured by the conventional method. As a control, a cream containing no germ extract was also produced by a conventional method.
(Composition) (wt%)
Propylene glycol 6.0
Dibutyl phthalate 19.0
Stearic acid 5.0
Glycerol monostearate 5.0
Sorbitan monostearate 12.0
Polyethylene sorbitan monostearate 38.0
Sodium edetate 0.03
Wheat germ extract (concentration 6 mg / mL) 1.0
Total amount of purified water is 100

(Example 31) Sensory evaluation Sensory evaluation was performed using Examples 28-30. In addition, the comparative example which does not contain an embryo extract was also evaluated simultaneously. For sensory evaluation, 20 panelists aged 40 to 60 who are worried about aging symptoms such as wrinkles are taken as a group, and the examples and comparative examples are used twice a day for 3 months continuously. A questionnaire survey was conducted on the skin condition.

  Table 5 shows the number of evaluators in each item as a result of sensory evaluation. More than 80% of the panel responded that the elasticity of the skin was recovered and the wrinkles were reduced, compared to the comparative example that did not contain the germ extract. It was shown that there is.

(Example 32)

I. Experimental example extracted with industrial grade raw material and polyphenol adsorbent, 1N or less hydrochloric acid 1kg each of soybean germ (soybean germ / powder type, manufactured by For You) 6L each 0.1N, 0.5N, 1N Hydrochloric acid solution was added. Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. The stirred product was centrifuged at 12,000 × g for 30 minutes at 4 ° C., and the liquid fraction was collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 12,000 × g for 30 minutes at 4 ° C. The liquid fraction was collected, and this liquid was used as a plant extract (soybean germ extract). The plant extract (soybean germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom), concentrated by freeze-drying, and used for various evaluations.

  From the above results, it can be confirmed that plant extracts containing cell activation and anti-aging components can be recovered even at hydrochloric acid concentrations of 0.1 N, 0.5 N, and 1 N, and there is a preparation method that is excellent in terms of safety and handling. It was found.

II. Experimental example extracted with industrial grade raw material and polyphenol adsorbent, 1N or less sulfuric acid 1kg each of soybean germ (soy germ / powder type, manufactured by For You) 6L each 0.1N, 0.5N, 1N Sulfuric acid solution was added. Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. Centrifugation is performed at 4 ° C. and 12,000 × g for 30 minutes, and a liquid fraction is collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 4 ° C. and 12,000 × g for 30 minutes. The liquid fraction was collected, and this liquid was used as a plant extract (soybean germ extract). In the plant extract with 0.1N sulfuric acid, the plant extract (soybean germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by freeze-drying, and used for various evaluations. .

  From the above results, it can be confirmed that plant extracts containing cell activating and anti-aging components can be recovered even at sulfuric acid concentrations of 0.1 N, 0.5 N, and 1 N, and a preparation method that is excellent in terms of safety and handling is also provided. It was found.

III. Experimental example of extraction with industrial grade raw material and polyphenol adsorbent, 1N or less hydrochloric acid 1kg of wheat germ (specially defatted wheat germ, fine powder type, 20kg, Nisshin Pharma Co., Ltd.) 1N, 0.5N, 1N hydrochloric acid solution was added. Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. Centrifugation is performed at 4 ° C. and 12,000 × g for 30 minutes, and a liquid fraction is collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 4 ° C. and 12,000 × g for 30 minutes. Then, the liquid fraction was collected, and this liquid was used as a plant extract (wheat germ extract). The plant extract (wheat germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  From the above results, it can be confirmed that plant extracts containing cell activating and anti-aging components can be recovered even at sulfuric acid concentrations of 0.1 N, 0.5 N, and 1 N, and a preparation method that is excellent in terms of safety and handling is also provided. It was found.

IV. Industrial grade raw material, polyphenol adsorbent, experimental example extracted with 1N or less sulfuric acid 1kg of wheat germ (specially defatted wheat germ, fine powder type, 20kg, Nisshin Pharma Co., Ltd.) A 1N, 0.5N, 1N sulfuric acid solution was added. Further, 80 g of each of Daibagan F (BASF), which is a polyphenol adsorbent, was added, and the mixture was stirred for 2 hours at room temperature with a three-one motor and extracted under acidic conditions. Centrifugation is performed at 4 ° C. and 12,000 × g for 30 minutes, and a liquid fraction is collected, neutralized with 30% sodium hydroxide solution, and then centrifuged at 4 ° C. and 12,000 × g for 30 minutes. Then, the liquid fraction was collected, and this liquid was used as a plant extract (wheat germ extract). The plant extract (wheat germ extract) was desalted with an electrodialyzer (Acylizer, manufactured by Astom Corp.), concentrated by lyophilization, and used for various evaluations.

  From the above results, it can be confirmed that plant extracts containing cell activating and anti-aging components can be recovered even at sulfuric acid concentrations of 0.1 N, 0.5 N, and 1 N, and a preparation method that is excellent in terms of safety and handling is also provided. It was found.

  According to the present invention, it is possible to provide a method for preparing a plant extract derived from a plant that is highly safe and contains an active ingredient in a large amount with a high yield, and further, the plant extract has a cell activation action, an anti-aging action, an extracellular action. It can be expected to promote matrix production, and can provide cosmetics and quasi-drugs (external preparations for skin, bath preparations, hair growth agents, etc.), foods and drinks, and pharmaceuticals that contain cell activation and anti-aging ingredients as active ingredients. It is expected to make a significant contribution to industry.

Claims (27)

  It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. Anti-aging agent.   It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. Cell activating agent.   It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. And an extracellular matrix production promoter.   It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. A collagen production promoter.   It contains a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean germ, wheat seed, wheat germ, wheat germ, wheat germ, soy milk, and okara. Hyaluronic acid production promoter.   A plant extract obtained from at least one selected from the group consisting of soybean seeds, soybean germs, soybean embryos, soybean germs, wheat seeds, wheat germs, wheat germs, wheat germs, soy milk, and okara is used as animal skin. The anti-aging method characterized by including the process made to contact.   A plant extract obtained from at least one selected from the group consisting of soybean seeds, soybean germs, soybean embryos, soybean germs, wheat seeds, wheat germs, wheat germs, wheat germs, soy milk, and okara is used as animal skin. The cell activation method characterized by including the process made to contact. The anti-aging agent according to claim 1, the cell activator according to claim 2, the extracellular matrix production promoter according to claim 3, the collagen production promoter according to claim 4, or the claim 5. Cosmetics characterized by containing a hyaluronic acid production promoter.   The anti-aging agent according to claim 1, the cell activator according to claim 2, the extracellular matrix production promoter according to claim 3, the collagen production promoter according to claim 4, or the claim 5. A quasi-drug containing a hyaluronic acid production promoter.   The anti-aging agent according to claim 1, the cell activator according to claim 2, the extracellular matrix production promoter according to claim 3, the collagen production promoter according to claim 4, or the claim 5. A food or drink comprising a hyaluronic acid production promoter.   The anti-aging agent according to claim 1, the cell activator according to claim 2, the extracellular matrix production promoter according to claim 3, the collagen production promoter according to claim 4, or the claim 5. A pharmaceutical product comprising a hyaluronic acid production promoter.   Contact the animal skin with a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara A method of promoting extracellular matrix production, comprising the step of:   Contact the animal skin with a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara A method for promoting collagen production, comprising the step of:   Contact the animal skin with a plant extract obtained from at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, soybean bud, wheat seed, wheat germ, wheat germ, wheat germ, soy milk and okara And a hyaluronic acid production promoting method comprising the step of:   (1) A method for preparing a plant extract, comprising a step of treating a plant and / or plant extract under acidic conditions, and (2) a step of separating a liquid fraction.   The method for preparing a plant extract according to claim 15, wherein an acid solution containing a mineral acid and / or an organic acid is added and the mixture is treated under acidic conditions.   16. The method for preparing a plant extract according to claim 15, wherein an acid solution containing a mineral acid and / or an organic acid is added so as to have a pH of 2 or less and the mixture is treated under acidic conditions.   Mineral acid and / or organic acid is hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trichloroacetic acid, perchloric acid, citric acid, lactic acid, propionic acid, butyric acid, succinic acid, malonic acid, succinic acid, fumaric acid, maleic acid The method for preparing a plant extract according to claim 16 or 17, which is at least one acid selected from the group consisting of malic acid, tartaric acid, benzoic acid, sulfosalicylic acid and formic acid.   The method for preparing a plant extract according to any one of claims 15 to 18, wherein the acid solution added so as to be in an acidic condition is hydrochloric acid and / or perchloric acid and / or sulfuric acid.   The plant extract according to any one of claims 15 to 19, wherein a polyphenol adsorbent is added and a liquid fraction is separated simultaneously with or after the plant and / or plant extract is treated under acidic conditions. Preparation method.   The plant and / or plant extract is at least one selected from the group consisting of soybean seed, soybean germ, soybean embryo, wheat seed, wheat germ, wheat embryo, soy milk, and okara. The preparation method of the plant extract in any one of 15-20.   The method for preparing a plant extract according to any one of claims 15 to 21, wherein the step of separating the liquid fraction is centrifugation and / or filtration separation.   The plant extract recovered by the preparation method according to any one of claims 15 to 22, further comprising at least one selected from the group consisting of an ion exchange method, a gel filtration method, a membrane fractionation method, and an electrodialysis method. A method for preparing a plant extract, which is purified by the treatment of   The cell activation agent or anti-aging agent containing the plant extract collect | recovered by the preparation method in any one of Claims 15-23.   The extracellular matrix production promoter containing the plant extract collect | recovered by the preparation method in any one of Claims 15-23.   The collagen production promoter containing the plant extract collect | recovered by the preparation method in any one of Claims 15-23.   The hyaluronic acid production promoter containing the plant extract collect | recovered by the preparation method in any one of Claims 15-23.

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