JPH10101624A - Production of polyamine - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a polyamine in a good efficiency and yield, having a physiological activity, and useful as a medicine, a cosmetic material, a food, etc., by extracting teas under an acidic condition. SOLUTION: This method for producing a polyamine is e.g. to add 25ml of 5% trichloroacetic acid to 5g green tea, agitate vigorously with a homogenizer, then keep agitating mildly for one hour, separate the obtained mixture to a supernatant and a precipitate with a centrifugal separator, pass the obtained supernatant through a week cation exchanging resin, then wash the resin sufficiently with water, and elute the polyamine adsorbed to the resin with 6N HCl for obtaining the polyamine from the tea.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for producing a polyamine.

[0002]

BACKGROUND OF THE INVENTION Polyamines (i.e., putrescine containing two molecules of amine, spermidine containing three molecules of amine, and spermine containing four molecules of amine) are about 200 years older than those of nucleic acids, but their physiology. It is only recently that activity has become somewhat apparent. And as its physiological activity, various studies are currently being conducted.
Various effects on living organisms, especially as an essential component of cells (eg, cell proliferation, cell differentiation, environmental adaptation, synthesis of proteins and nucleic acids, stabilization of cell membranes, neurotransmission, etc.), and intracellular antioxidant properties And chemical antioxidant and anti-Maillard reactivity. Furthermore, it is said to be effective also in the diagnosis of cancer. (For example, for polyamines in general, Kazue Igarashi, "Mysterious Biological Substances-Polyamines", Kyoritsu Shuppan, 1993;
Lφvaas "Antioxidative Effe
cts of Polyamines " A. O.
C. S. 68, 353-358, 1991). These polyamines are widely found in biological tissues and the like.

[0003]

SUMMARY OF THE INVENTION The present invention relates to a method for obtaining polyamines having various bioactive actions. That is, the present invention relates to a method for producing a polyamine by extracting a polyamine from teas under acidic conditions. In the present invention, tea is used as a raw material. The teas used in the present invention can be obtained from leaves and / or stems of tea plants, which are subtropical plants of the genus Camellia, and can be either raw or processed. The tea plant is Chinese C. sinensis var
sinensis. And Assam species C.I. sin. va
rassamica, which is broadly classified into two varieties, and further includes an Assam hybrid which is an intermediate between the two varieties. These leaves and / or stems are processed into teas such as green tea (Japanese tea), oolong tea, and black tea. In the present invention, these teas can be used, but green tea is preferred.
In addition, teas themselves or so-called tea husks remaining after extraction with hot water can be used. Further, as the shape of tea, powdered tea can also be used.

[0004] As described above, polyamines are widely present in living organisms, but in teas, three types of compounds contained in polyamines at high concentrations are present on average. For example, in green tea, putrescine (A) contains 520 nmol /
g, spermidine (B) at 885 nmol / g and spermine (C) at 552 nmol / g. In contrast, in the same plant rice, A <10 nmol / g, B 27 nmol / g, and C <20 nmol / g.
In the flour, A is 17 nmol / g, B is 66 nmol / g, and C is 26 nmol / g. In soybean, A is 200-500 nmol / g, and B is 400-8 nmol / g.
00 nmol, C is 40-200 nmol / g,
In Shiitake mushrooms, A was 29 nmol / g and B was 893 nmol / g.
g and C do not exist. Furthermore, in orange, A is 89
Although it is as large as 0-1331 nmol / g, B is as small as 18-26 nmol / g and C is as small as <5 nmol / g. Regarding animals, in beef, A is <5 nmol / g, B is 18 nmol / g, and C is 136 nmol / g. In pork, A is 13 nmol / g, B is 18 nmol / g, C is 18 nmol / g.
Is 136 nmol / g, in chicken, A is <5 nmol / g, B is 20 nmol / g, and C is 308 nmol / g. In tuna meat, A is not present and B is 30 nmol / g. g and C are 34 nmol / g. In chicken eggs,
None of A, B and C are present. As can be seen from this data, tea contains a high concentration of any of the three compounds that make up the polyamine, so that extraction from it results in a high yield of the resulting polyamine. For this reason, extracting polyamines from teas is industrially superior. In particular, it can be said that the ability to use tea husks that are conventionally produced as a large amount of waste is also advantageous from an environmental point of view.

In the present invention, a polyamine is extracted from this tea under acidic conditions. In this extraction, a conventional method can be used. For example, as an example,
Tea is extracted in an organic solvent, and the extract is passed through a cation exchange resin to adsorb the polyamine to the resin. The polyamine adsorbed on the resin is eluted with an acid such as hydrochloric acid. The obtained eluate is purified by high performance liquid chromatography. Alternatively, teas are extracted with a polyamine by an acidic solution containing an inorganic acid such as hydrochloric acid or an organic acid such as acetic acid, and water is removed from the resulting solution containing the polyamine by means of heating or the like, and the tea is treated with an alkali. Neutralize to obtain a liquid containing a high concentration of polyamine, or evaporate the liquid to dryness to obtain a solid. This method is suitable for obtaining low-purity polyamines such as food applications from tea leaves. Any method other than these methods can be used. In the present invention, a polyamine can be obtained in a high yield.
Since the obtained polyamine has the above-mentioned physiological activities, it can be used for applications such as pharmaceuticals, cosmetics, and foods, taking advantage of the characteristic of being a natural product.

[0006]

【Example】

Example 1 To 5 g of green tea, 25 mL of 5% trichloroacetic acid was added and stirred vigorously with a homogenizer, followed by gentle stirring for another hour. The obtained mixture was separated into a supernatant and a precipitate by a centrifuge (15,000 rotations / 1 minute, 15 minutes). 100 μL of the obtained supernatant was added to Muroma
c After passing through a CR-70 column (weak cation exchange resin, equilibrated with 2 mM acetate buffer pH 5.0), the plate was washed thoroughly with water, and the polyamine adsorbed on the resin was eluted with 6N HCl. The solvent was evaporated to dryness using an evaporator, and 400 μL of water was added to dissolve the solid. 40 μL of the mixture was subjected to high performance liquid chromatography (column: Tosoh, TSK-Kel Aminopak, eluent: 6% ethanol, 0.2M NaCl containing 2.4 M NaCl).
2M acetate buffer (pH 5.6, 65 ° C.) to separate the purified polyamine. The obtained polyamine is green tea 1
Each g contained 46 μg putrescine, 128 μg spermidine, 112 μg spermine.

Claims (1)

[Claims] 1. A method for producing a polyamine, comprising extracting a polyamine from teas under acidic conditions.