CN102048026A - Preparation method of oil-tea camellia meal protein polypeptide as additive for functional feeds - Google Patents
Preparation method of oil-tea camellia meal protein polypeptide as additive for functional feeds Download PDFInfo
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- CN102048026A CN102048026A CN2010105729221A CN201010572922A CN102048026A CN 102048026 A CN102048026 A CN 102048026A CN 2010105729221 A CN2010105729221 A CN 2010105729221A CN 201010572922 A CN201010572922 A CN 201010572922A CN 102048026 A CN102048026 A CN 102048026A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention relates to a preparation method of an oil-tea camellia meal protein polypeptide as an additive for functional feeds, comprising the following steps of, firstly, crushing a oil-tea camellia cake; secondly, removing saponin; thirdly, adding compound enzyme to carry out hydrolysis; fourthly, inactivating enzyme at the temperature of 80-90 DEG C for 5-15min; fifthly, carrying out centrifugal separation, and collecting supernate; sixthly, regulating a pH value of the supernate to be neutral; seventhly, filtering with a microporous membrane; and eighthly, carrying out low-temperature vacuum drying with the vacuum degree lower than 0.098MPa at the temperature of 35-55 DEG C to obtain the oil-tea camellia meal protein polypeptide. In the invention, the prepared oil-tea camellia meal protein polypeptide has the relative molecular mass lower than 1000Da, belongs to small molecular peptides, is safe to eat without bitter taste and toxicity, has strong immuno-modulating activity, and can efficiently enhance the survival rate and the weight growth rate of raised animals. The method provided by the invention can comprehensively utilize the agriculture waste resources, change the wastes into valuable and be beneficial to environmental protection, and has the advantages of low production cost and remarkable economic and social benefits.
Description
Technical field
The present invention relates to the preparation method of a kind of feed, relate in particular to the preparation method of a kind of functional feed with additive oil tea cake protein polypeptide with functional additive.
Background technology
At present, the accessory substance albumen after the processing of fish meal and animal is most widely used protein sources in livestock and poultry and the aquatic feeds always.But the problem that has three aspects: the first because be subjected to global to excessively capture, EI Nino phenomenon and environmental pollution etc. influence, the output of fish meal descends year by year, the supply of world's fish meal at present can not have been satisfied growing breed demand; The second, in order to take precautions against the invasion of the spongy virus of ox brain, animal derived feed such as digested tankage are prohibited in many countries and regions, bone meal, and animal proteins such as meat meal tankage, blood meal, plasma powder, feather meal, whey powder are used in animal and fowl fodder; The 3rd, China's animal protein raw material resources are shortage very, mostly dependence on import.The price of high-quality animal-based protein is constantly soaring, has badly influenced the profit level of China's aquaculture.Oil tea cake protein polypeptide is to be raw material dry gained after the compound protein enzyme hydrolysis with the oil tea dregs of rice, this polypeptide has following advantage: it is rich in glutamic acid, arginine, leucine and aspartic acid (1), and amino acid is formed comprehensively, the fish albumen that its biological value (BV) and protein effect are used always near feed than (PER); (2) oil tea cake protein polypeptide is easier is digested and assimilated by animal, can improve the utilization rate of feed, reduces production costs; (3) oil tea cake protein polypeptide has the excellent function activity, it has very strong antioxidation activity on the one hand, on the other hand, tea seed polypeptide also has stronger immunoregulatory activity, can effectively improve by the resistance against diseases of letting animals feed, reduce the use of medicine, thereby improve greatly by the security of letting animals feed meat.Therefore oil tea cake protein polypeptide is a kind of feed addictive that is suitable as very much high economic worth animal, can add in the feed such as South America prawn, Tilapia mossambica etc., as the senior substitute of the conventional albumen of feed.
The present oil meal protein polypeptide method commonly used for preparing is " using the protease hydrolytic method again with the heavy albumen that extracts of the molten acid of alkali earlier " or " grouts are directly used the microbial inoculant fermentation method that produces protease ", the deadly defect of these methods has three: the protein denaturation that extract (1) is serious, poorly water-soluble causes the efficient of follow-up protease hydrolyzed low; (2) environmental pollution is serious, and after the heavy method of the molten acid of alkali was extracted albumen, waste liquid is difficult, and was big to ambient influnence; (3) grouts are directly used the microbial inoculant fermentation method that produces protease, this method is because the proteinase activity that obtains is not strong, and hydrolysis ability is limited, and degree of hydrolysis is wayward, and fermentation time is long, and production efficiency is low.
Summary of the invention
The objective of the invention is to overcome the above-mentioned defective that existing polypeptide preparation method exists, provide a kind of protein denaturation few, water-soluble good, production efficiency height, the functional feed that environmental pollution the is few preparation method of additive oil tea cake protein polypeptide.
The objective of the invention is to be achieved through the following technical solutions, it may further comprise the steps:
(1) grouts of tea seed after oil expression is pulverized with pulverizer, crossed 100 mesh sieves;
(2) use water extract method, according to solid masses and the ratio of liquid volume ratio for 1:7 ~ 13 (preferred 1:8 ~ 12), carrying out Tea Saponin separates, extraction temperature is 65 ~ 85 ℃ (preferred 70 ~ 80 ℃), extraction time is 6 ~ 10 h (preferred 7 ~ 9 h), and leaching liquor pH value is 7.0, and is centrifugal after lixiviate is finished, leaching liquor is separated with the tea dregs of rice, obtain removing the oil tea dregs of rice of Tea Saponin;
(3) according to solid masses and the ratio of liquid volume ratio for 1:7 ~ 13 (preferred 1:8 ~ 12), to take off in the saponin oil tea dregs of rice adding clear water and soak 1 ~ 3 h (preferred 2 h), regulate pH value to 8.0, adding is the compound protease that 2 ~ 4:1 (preferred 3:1) forms by alkali protease and flavor protease by mass ratio, the complex enzyme consumption places 45 ~ 55 ℃ of temperature (preferred 50 ℃) shaking bath hydrolysis 12 ~ 16 h (preferred 13 ~ 14 h) for removing 1 ~ 3 wt% (preferred 1.5 ~ 2.5 wt%) of the contained protein content of the oil tea dregs of rice (measuring with Kjeldahl) of Tea Saponin;
(4) after hydrolysis is finished, hydrolysate is heated to 80 ~ 90 ℃ (preferred 85 ℃), keeps 5 ~ 15 min (preferred 8 ~ 12 min), make enzyme deactivation;
(5) centrifugation solid-liquid phase is collected supernatant;
(6) supernatant pH value is adjusted to neutrality;
(7) be that the microporous barrier (optimization polypropylene macromolecular ultrafine fibrous membrane) of 0.1 ~ 1 μ m filters with the aperture, remove big molecule;
(8) place vacuum to be lower than 0.098 MPa, 35 ~ 55 ℃ of (preferred 40 ℃) low-temperature vacuum dryings, obtaining relative molecular mass is the following oil tea cake protein polypeptide of 1000 Da.
Advantage of the present invention is that production cost is low, and the product that uses the inventive method to obtain is used for culturing, and animal survival rate, rate of body weight gain significantly improve, and immunity obviously strengthens, and culture efficiency is obvious; Have no side effect; Can make the agricultural wastes resource obtain comprehensive utilization, turn waste into wealth, help environmental protection, economic and social benefit is remarkable.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
(1) grouts of tea seed after oil expression is pulverized with pulverizer, crossed 100 mesh sieves;
(2) use water extract method, than being the ratio of 1:8, carrying out Tea Saponin and separate according to solid masses and liquid volume, extraction temperature is 70 ℃, and extraction time is 9 h, and leaching liquor pH value is 7.0, centrifugal after lixiviate is finished, leaching liquor is separated with the tea dregs of rice, obtain removing the oil tea dregs of rice of Tea Saponin;
(3) according to solid masses and liquid volume than being the ratio of 1:8, to take off the saponin oil tea dregs of rice and add immersion 2 h in the clear water, regulate pH value to 8.0, adding is the compound protease that 3:1 forms by flavor protease and alkali protease by mass ratio, the complex enzyme consumption places 50 ℃ of shaking bath hydrolysis 14 h of temperature for removing 1.5 wt% of the contained protein content of the oil tea dregs of rice (measuring with Kjeldahl) of Tea Saponin;
(4) after hydrolysis is finished, hydrolysate is heated to 85 ℃, keeps 8 min, make enzyme deactivation;
(5) centrifugation solid-liquid phase is collected supernatant;
(6) hydrochloric acid with 0.5 mol/L is adjusted to neutrality with supernatant pH value;
(7) be that the polypropylene macromolecular ultrafine fibrous membrane of 0.1 ~ 1 μ m filters with the aperture, remove big molecule;
(8) place vacuum to be lower than 0.098 MPa, 40 ℃ of low-temperature vacuum dryings obtain the oil tea cake protein polypeptide that relative molecular mass is lower than 1000 Da.
Embodiment 2
(1) grouts of tea seed after oil expression is pulverized with pulverizer, crossed 100 mesh sieves;
(2) use water extract method, than being the ratio of 1:12, carrying out Tea Saponin and separate according to solid masses and liquid volume, extraction temperature is 80 ℃, and extraction time is 7 h, and leaching liquor pH value is 7.0, centrifugal after lixiviate is finished, leaching liquor is separated with the tea dregs of rice, obtain removing the oil tea dregs of rice of Tea Saponin;
(3) according to solid masses and liquid volume than being the ratio of 1:12, to take off the saponin oil tea dregs of rice and add immersion 2.0 h in the clear water, regulate pH value to 8.0, adding is the compound protease that 3:1 forms by flavor protease and alkali protease by mass ratio, the complex enzyme consumption places 50 ℃ of shaking bath hydrolysis 13 h of temperature for removing 2.5 wt% of the contained protein content of the oil tea dregs of rice (measuring with Kjeldahl) of Tea Saponin;
(4) after hydrolysis is finished, hydrolysate is heated to 85 ℃, keeps 12 min, make enzyme deactivation;
(5) centrifugation solid-liquid phase is collected supernatant;
(6) hydrochloric acid with 0.5 mol/L is adjusted to neutrality with supernatant pH value;
(7) be that the polypropylene macromolecular ultrafine fibrous membrane of 0.1 ~ 1 μ m filters with the aperture, remove big molecule;
(8) place vacuum to be lower than 0.098 MPa, 40 ℃ of low-temperature vacuum dryings obtain the oil tea cake protein polypeptide that relative molecular mass is lower than 1000 Da.
Embodiment 3
Adding the oil tea cake protein polypeptide of 500 mg embodiment, 1 preparation in every kilogram of feed feeds 5 weeks of Penaeus Vannmei, compare with basal feed group (not adding oil tea cake protein polypeptide), its survival rate has improved 15.2%, rate of body weight gain has improved 25.4%, (the serum lysozyme vigor has improved 49.1% in the immunity enhancing, the acid phosphatase enzyme activity has improved 63.7%, alkaline phosphatase activity has improved 59.3%, superoxide dismutase activity has improved 61.5%, mda content has reduced by 21.6%, the vigor of described enzyme and MDA content all adopt Nanjing to build up the corresponding reagent box that bio-engineering research is produced, and measure with AAS).
Embodiment 4
Adding the oil tea cake protein polypeptide of 1000 mg embodiment, 1 preparation in every kilogram of feed feeds 5 weeks of Penaeus Vannmei, compare with basal feed group (not adding oil tea cake protein polypeptide), its survival rate has improved 20.4%, rate of body weight gain has improved 32.6%, (the serum lysozyme vigor has improved 56.8% in the immunity enhancing, the acid phosphatase enzyme activity has improved 75.9%, alkaline phosphatase activity has improved 70.8%, superoxide dismutase activity has improved 76.2%, mda content has reduced by 27.9%, the vigor of described enzyme and MDA content all adopt Nanjing to build up the corresponding reagent box that bio-engineering research is produced, and measure with AAS).
Embodiment 5
Adding the oil tea cake protein polypeptide of 1500 mg embodiment, 2 preparations in every kilogram of feed feeds 5 weeks of Penaeus Vannmei, compare with basal feed group (not adding oil tea cake protein polypeptide), its survival rate has improved 24.7%, rate of body weight gain has improved 38.8%, (the serum lysozyme vigor has improved 60.3% in the immunity enhancing, the acid phosphatase enzyme activity has improved 82.1%, alkaline phosphatase activity has improved 76.8%, superoxide dismutase activity has improved 83.4%, mda content has reduced by 33.7%, the vigor of described enzyme and MDA content all adopt Nanjing to build up the corresponding reagent box that bio-engineering research is produced, and measure with AAS).
Embodiment 6
Adding the oil tea cake protein polypeptide of 2000 mg embodiment, 2 preparations in every kilogram of feed feeds 5 weeks of Penaeus Vannmei, compare with basal feed group (not adding oil tea cake protein polypeptide), its survival rate has improved 26.3%, rate of body weight gain has improved 41.2%, (the serum lysozyme vigor has improved 63.1% in the immunity enhancing, the acid phosphatase enzyme activity has improved 84.9%, alkaline phosphatase activity has improved 78.5%, superoxide dismutase activity has improved 85.1%, mda content has reduced by 35.2%, the vigor of described enzyme and MDA content all adopt Nanjing to build up the corresponding reagent box that bio-engineering research is produced, and measure with AAS).
Claims (6)
1. a functional feed is characterized in that may further comprise the steps with the preparation method of additive oil tea cake protein polypeptide:
(1) grouts of tea seed after oil expression is pulverized with pulverizer, crossed 100 mesh sieves;
(2) use water extract method, than being the ratio of 1:7 ~ 13, carrying out Tea Saponin and separate according to solid masses and liquid volume, extraction temperature is 65 ~ 85 ℃, and extraction time is 6 ~ 10 h, and leaching liquor pH value is 7.0, centrifugal after lixiviate is finished, leaching liquor is separated with the tea dregs of rice, obtain removing the oil tea dregs of rice of Tea Saponin;
(3) according to solid masses and liquid volume than being the ratio of 1:7 ~ 13, to take off in the saponin oil tea dregs of rice adding clear water and soak 1 ~ 3 h, regulate pH value to 8.0, adding is the compound protease that 2 ~ 4:1 forms by alkali protease and flavor protease by mass ratio, place 45 ~ 55 ℃ of shaking bath hydrolysis 12 ~ 16 h of temperature, the complex enzyme consumption is for removing 1 ~ 3 wt% of the contained protein content of the oil tea dregs of rice of Tea Saponin, and the contained protein content of the oil tea dregs of rice that has removed Tea Saponin is measured with Kjeldahl;
(4) after hydrolysis is finished, hydrolysate is heated to 80 ~ 90 ℃, keeps 5 ~ 15 min, make enzyme deactivation;
(5) centrifugation solid-liquid phase is collected supernatant;
(6) supernatant pH value is adjusted to neutrality;
(7) be that the microporous barrier of 0.1 ~ 1 μ m filters with the aperture, remove big molecule;
(8) place vacuum to be lower than 0.098 MPa, 35 ~ 55 ℃ of low-temperature vacuum dryings obtain oil tea cake protein polypeptide.
2. the functional feed according to claim 1 preparation method of additive oil tea cake protein polypeptide, it is characterized in that the described water extract method of step (2), according to solid masses and liquid volume than being the ratio of 1:8 ~ 12, carrying out Tea Saponin separates, extraction temperature is 70 ~ 80 ℃, and extraction time is 7 ~ 9 h.
3. the functional feed according to claim 1 preparation method of additive oil tea cake protein polypeptide, it is characterized in that step (3) described according to solid masses and liquid volume than being the ratio of 1:8 ~ 12, to take off the saponin oil tea dregs of rice and add immersion 2 h in the clear water, adding is the compound protease that 3:1 forms by alkali protease and flavor protease by mass ratio, the complex enzyme consumption places 50 ℃ of shaking bath hydrolysis 13 ~ 14 h of temperature for removing 1.5 ~ 2.5 wt% of the contained protein content of the oil tea dregs of rice of Tea Saponin.
4. functional feed according to claim 1 is with the preparation method of additive oil tea cake protein polypeptide, it is characterized in that the described hydrolysis of step (4) is finished after, hydrolysate is heated to 85 ℃, keep 8 ~ 12 min, make enzyme deactivation.
5. functional feed according to claim 1 is with the preparation method of additive oil tea cake protein polypeptide, it is characterized in that step (7) described be that the polypropylene macromolecular ultrafine fibrous membrane of 0.1 ~ 1 μ m filters with the aperture, remove big molecule.
6. functional feed according to claim 1 is characterized in that with the preparation method of additive oil tea cake protein polypeptide the described vacuum that places of step (8) is lower than 0.098 MPa, and 40 ℃ of low-temperature vacuum dryings obtain oil tea cake protein polypeptide.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484517A (en) * | 2013-09-11 | 2014-01-01 | 邹远东 | Camellia seed protein peptide, preparation method and application thereof |
| CN104450846A (en) * | 2014-12-05 | 2015-03-25 | 中南林业科技大学 | Semi-continuous preparation method of antihypertensive peptide of oil-tea meal protein |
| CN104531814A (en) * | 2014-12-05 | 2015-04-22 | 中南林业科技大学 | Preparation method of oil-tea meal enzymatically-modified protein |
| CN107259101A (en) * | 2017-02-13 | 2017-10-20 | 中南林业科技大学 | A kind of method of camellia seed meal ferment making feed |
| CN108060196A (en) * | 2017-12-11 | 2018-05-22 | 盛蓓蓓 | A kind of Extracted From Oil-tea-cake polypeptide, preparation method and the effect for promoting weary feelings estrus of cow and fertilization |
| WO2019114159A1 (en) * | 2017-12-11 | 2019-06-20 | 盛蓓蓓 | Camellia oleifera meal polypeptide and polysaccharide as well as preparation method and application thereof, and camellia oleifera meal detoxification method |
| CN114686552A (en) * | 2022-04-02 | 2022-07-01 | 吉林农业大学 | Preparation method of camellia seed meal polypeptide zinc chelate |
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| CN1439720A (en) * | 2003-01-30 | 2003-09-03 | 大连理工大学 | Soya protein oligopeptides production |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484517A (en) * | 2013-09-11 | 2014-01-01 | 邹远东 | Camellia seed protein peptide, preparation method and application thereof |
| CN103484517B (en) * | 2013-09-11 | 2016-03-02 | 邹远东 | A kind of camellia seed protein peptide and preparation method thereof and its application |
| CN104450846A (en) * | 2014-12-05 | 2015-03-25 | 中南林业科技大学 | Semi-continuous preparation method of antihypertensive peptide of oil-tea meal protein |
| CN104531814A (en) * | 2014-12-05 | 2015-04-22 | 中南林业科技大学 | Preparation method of oil-tea meal enzymatically-modified protein |
| CN104531814B (en) * | 2014-12-05 | 2017-11-14 | 中南林业科技大学 | A kind of preparation method of Extracted From Oil-tea-cake enzyme modification albumen |
| CN104450846B (en) * | 2014-12-05 | 2018-05-01 | 中南林业科技大学 | A kind of Extracted From Oil-tea-cake protein blood pressure-reducing peptide semi continuous preparation method |
| CN107259101A (en) * | 2017-02-13 | 2017-10-20 | 中南林业科技大学 | A kind of method of camellia seed meal ferment making feed |
| CN108060196A (en) * | 2017-12-11 | 2018-05-22 | 盛蓓蓓 | A kind of Extracted From Oil-tea-cake polypeptide, preparation method and the effect for promoting weary feelings estrus of cow and fertilization |
| CN108060196B (en) * | 2017-12-11 | 2018-11-27 | 正大联合动物制药科技（江苏）有限公司 | A kind of Extracted From Oil-tea-cake polypeptide, preparation method and the effect for promoting weary feelings estrus of cow and fertilization |
| WO2019114159A1 (en) * | 2017-12-11 | 2019-06-20 | 盛蓓蓓 | Camellia oleifera meal polypeptide and polysaccharide as well as preparation method and application thereof, and camellia oleifera meal detoxification method |
| CN114686552A (en) * | 2022-04-02 | 2022-07-01 | 吉林农业大学 | Preparation method of camellia seed meal polypeptide zinc chelate |
| CN114686552B (en) * | 2022-04-02 | 2024-04-30 | 吉林农业大学 | Preparation method of camellia seed meal polypeptide zinc chelate |
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