CN101570773A - Method for producing active polypeptide by microwave aid enzymolysis of oil meal protein - Google Patents
Method for producing active polypeptide by microwave aid enzymolysis of oil meal protein Download PDFInfo
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Abstract
The invention relates to a method for preparing active polypeptide. The method for producing active polypeptide by microwave aid enzymolysis of oil meal protein is characterized in that the method comprises the following steps: 1) preparation of raw materials: crushing the meal protein, and then sieving the meal protein by a sieve of 20 to 60 meshes to obtain meal protein powder; 2) microwave pretreatment: according to a ratio of the meal protein powder to buffer solution being 1 gram to 20 to 30 milliliters, selecting the meal protein powder and the buffer solution, placing the meal protein powder into a microwave reaction bottle, then adding the buffer solution into the microwave reaction bottle, putting the microwave reaction bottle into a microwave device, reacting for 2 to 4 minutes at the temperature between 50 and 80 DEG C under 600 to 800 watts, and taking out a reaction product to obtain a microwave pretreatment material; 3) adopting one of the following nine modes to carry out microwave enzymolysis to obtain enzymolysis solution; and 4) inactivating the enzymolysis solution to obtain the active polypeptide. The method has the advantages of simple process, low cost and environmental protection, and can be applicable to industrialized production.
Description
Technical field
The present invention relates to a kind of preparation method of active polypeptide.
Background technology
Microwave is a kind of frequency electromagnetic waves, and the polar molecule in the microwave field is in the shimmy state of high speed, and the result of molecular motion has caused collision of molecules and friction aggravation, thereby produces a large amount of heats.The advantage of microwave heating is inside and outside the medium characteristics of heating simultaneously, be all chain carriers that microwave can be given transmission ofenergy medium simultaneously, therefore than other heating systems more active centre is arranged, molecule bumps and is reflected at moment and finishes, so speed of response improves greatly.
The oil meal of low price, high protein is mainly as the feedstuff protein raw material.Oil meal is degraded to micromolecule polypeptide, and its solvability, emulsifying property, retentiveness etc. all are better than oilseed protein, also have nutritional-physiological performances such as absorption is fast, specific absorption height.Low molecular peptide has low antigenicity, can not produce anaphylaxis, can be used as sports nutrition agent, diet food, old people food etc.
Summary of the invention
The object of the present invention is to provide a kind of microwave-assisted enzymolysis oil meal protein to produce the method for active polypeptide, this method technology is simple, the reaction times short.
To achieve these goals, technical scheme of the present invention is: microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, it is characterized in that it comprises the steps:
1) preparation of raw material: cake protein is pulverized, crossed 20～60 mesh sieves then, obtain the cake protein powder;
2) Microwave Pretreatment:
Solid-liquid ratio by cake protein powder and damping fluid is 1g: 20～30mL, chooses cake protein powder and damping fluid, and the cake protein powder is placed the microwave reaction bottle, add damping fluid, be put in the microwave device, 50～80 ℃, the following reaction of 600～800W 2～4min, take out, obtain the Microwave Pretreatment material;
3) adopt one of following 9 kinds of modes to carry out the microwave enzymolysis:
1. be 8.0 with Microwave Pretreatment material adjust pH, add Sumizyme MP, Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
2. be 7.0 with Microwave Pretreatment material adjust pH, add flavor protease, flavor protease with the ratio of Microwave Pretreatment material is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
3. be 7.0 with Microwave Pretreatment material adjust pH, add neutral protease, neutral protease with the ratio of Microwave Pretreatment material is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
4. be 8.0 with Microwave Pretreatment material adjust pH, add Sumizyme MP, Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
5. be 8.0 with Microwave Pretreatment material adjust pH, add Sumizyme MP, Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material A is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
6. be 7.0 with Microwave Pretreatment material adjust pH, add flavor protease, flavor protease with the ratio of Microwave Pretreatment material is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material B;
With microwave enzymolysis material B adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material B is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
7. be 7.0 with Microwave Pretreatment material adjust pH, add flavor protease, flavor protease with the ratio of Microwave Pretreatment material is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material B;
With microwave enzymolysis material B adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of microwave enzymolysis material B is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
8. be 7.0 with Microwave Pretreatment material adjust pH, add neutral protease, neutral protease with the ratio of Microwave Pretreatment material is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material C;
With microwave enzymolysis material C adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of microwave enzymolysis material C is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
9. be 7.0 with Microwave Pretreatment material adjust pH, add neutral protease, neutral protease with the ratio of Microwave Pretreatment material is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material C;
With microwave enzymolysis material C adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material C is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Described cake protein is any one or any two kinds mixing in rapeseed cake dregs, soybean cake dregs, the peanut dregs, and any two kinds is any proportioning when mixing.
Described damping fluid is that the pH value is 8.0 phosphoric acid, boric acid or Tris (Tutofusin tris) damping fluid.
Utilize that several proteolytic enzyme are compound to carry out enzymolysis to grouts, not only can promote proteinic stripping in the grouts, improve the nitrogen yield, and can improve degree of hydrolysis, the hydrophobic amino acid hydrolysis of little peptide end is formed total free aminoacids, thereby reduce or eliminate the bitter taste of hydrolysate, obtain the ideal hydrolysate.China is the country of protein famine, utilizes enzyme engineering technology that a large amount of cheap grouts are converted into human fine food source and has the important social economic implications.
The present invention compares with the traditional heating method, and microwave-assisted enzymolysis oil meal protein is produced active polypeptide 3 advantages, 1) the reaction times weak point, microwave can carry out in accelerated reaction; 2) utilization ratio of raising enzyme; The ordinary method required time is long, heating up all can cause enzyme deactivation slowly, and microwave heating can reach temperature required at short notice, finishes hydrolysis reaction rapidly; 3) improve proteic degree of hydrolysis, conventional heating hydrolysis method can cause protein aggregation and stop further hydrolysis, and microwave radiation can be destroyed protein aggregation, sex change, and to hydrophilic and hydrophobin hydrolyzable all.
The invention has the beneficial effects as follows:
1) technology is simple: technology preface of the present invention is simple, does not need complex apparatus; Utilize microwave device to get final product realization response.
2) reduce cost: utilize microwave to react, reach temperature required in the short period of time, improved the utilization ratio of enzyme, saved the cost of enzyme.
3) utilize microwave technology to be applied to the complex enzyme zymohydrolysis of grouts, proteolysis is thorough, utilization ratio is high.
4) reaction times is short: utilize microwave to carry out enzymolysis, the reaction times dropped to several minutes by several hours, and the reaction times greatly shortens.
Embodiment
In order to understand the present invention better, further illustrate content of the present invention below in conjunction with embodiment, but content of the present invention not only is confined to the following examples.
Embodiment 1:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: rapeseed cake dregs (or claiming the rapeseed cake cake protein) is pulverized, crossed 40 mesh sieves then, obtain the rapeseed cake dregs protein powder;
2) Microwave Pretreatment:
15g rapeseed cake dregs protein powder is placed the microwave reaction bottle of 500mL, add 400mL phosphoric acid buffer (pH value is 8.0), be put in the microwave device, 75 ℃, 600W are reaction 3min down, and taking-up obtains the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 4000U/g; Place microwave device then, 50 ℃, the following reaction of 500W 5min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 75LAPU/g; Place microwave device then, 50 ℃, the following reaction of 500W 5min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Active polypeptide is 42.6% through last mensuration degree of hydrolysis.
Embodiment 2:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: rapeseed cake dregs (or claiming the rapeseed cake cake protein) is pulverized, crossed 40 mesh sieves then, obtain the rapeseed cake dregs protein powder;
2) Microwave Pretreatment:
10g rapeseed cake dregs protein powder is placed the microwave reaction bottle of 400mL, add 250mL borate buffer (pH value is 8.0), be put in the microwave device, 55 ℃, 800W is reaction 2min down, and taking-up obtains the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 6000U/g; Place microwave device then, 40 ℃, the following reaction of 400W 10min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 100LAPU/g; Place microwave device then, 50 ℃, the following reaction of 400W 10min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Active polypeptide is 52.1% through last mensuration degree of hydrolysis.
Embodiment 3:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: peanut dregs (or claiming peanut dregs albumen) is pulverized, crossed 40 mesh sieves then, obtain the peanut dregs protein powder;
2) Microwave Pretreatment:
5g peanut dregs albumen is placed 200mL microwave reaction bottle, add 150mL phosphoric acid buffer (pH value 8.0), be put in the microwave device, 60 ℃, the following reaction of 700W 3min take out, and obtain the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 8000U/g; Place microwave device then, 40 ℃, the following reaction of 500W 8min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 50LAPU/g; Place microwave device then, 50 ℃, the following reaction of 400W 15min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Active polypeptide is 31.6% through last mensuration degree of hydrolysis.
Embodiment 4:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: rapeseed cake dregs (or claiming the rapeseed cake cake protein) is pulverized, crossed 40 mesh sieves then, obtain the rapeseed cake dregs protein powder;
2) Microwave Pretreatment:
10g rapeseed cake dregs protein powder is placed the microwave reaction bottle of 400mL, add 200mLTris (Tutofusin tris) damping fluid (pH value is 8.0), be put in the microwave device, 75 ℃, 600W is reaction 4min down, and taking-up obtains the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 5000U/g; Place microwave device then, 50 ℃, the following reaction of 400W 10min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 50LAPU/g; Place microwave device then, 55 ℃, the following reaction of 600W 10min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Active polypeptide is 39.6% through last mensuration degree of hydrolysis.
Embodiment 5:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: the preparation of raw material: rapeseed cake dregs (or claiming the rapeseed cake cake protein) is pulverized, crossed 60 mesh sieves then, obtain the rapeseed cake dregs protein powder;
2) Microwave Pretreatment:
20g rapeseed cake dregs protein powder is placed the microwave reaction bottle of 1000mL, add 500mL phosphoric acid buffer (pH value is 8.0), be put in the microwave device, 75 ℃, 700W are reaction 3min down, and taking-up obtains the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 4000U/g; Place microwave device then, 50 ℃, the following reaction of 500W 8min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material A is: 2000U/g; Place microwave device then, 50 ℃, the following reaction of 400W 15min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Active polypeptide is 43.5% through last mensuration degree of hydrolysis.
Embodiment 6:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: the preparation of raw material: rapeseed cake dregs (or claiming the rapeseed cake cake protein) is pulverized, crossed 20 mesh sieves then, obtain the rapeseed cake dregs protein powder;
2) Microwave Pretreatment:
10g rapeseed cake dregs protein powder is placed the microwave reaction bottle of 400mL, add 250mL borate buffer (pH value is 8.0), be put in the microwave device, 65 ℃, 600W are reaction 3min down, and taking-up obtains the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 3000U/g; Place microwave device then, 50 ℃, the following reaction of 400W 10min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 100LAPU/g; Place microwave device then, 60 ℃, the following reaction of 500W 15min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Active polypeptide is 45.85 through last mensuration degree of hydrolysis.
Embodiment 7:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: soybean cake dregs (or claiming soybean cake dregs albumen) is pulverized, crossed 20 mesh sieves then, obtain the soybean cake dregs protein powder;
2) Microwave Pretreatment:
Solid-liquid ratio by soybean cake dregs protein powder and phosphoric acid buffer is 1g: 20mL, choose soybean cake dregs protein powder and phosphoric acid buffer, the soybean cake dregs protein powder is placed the microwave reaction bottle, add phosphoric acid buffer (the pH value is 8.0), be put in the microwave device, 50 ℃, the following reaction of 600W 2min take out, and obtain the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000U/g; Place microwave device then, 40 ℃, 400W is reaction 5min down, takes out, and gets microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material A is: 500U/g; Place microwave device then, 40 ℃, the following reaction of 400W 5min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Embodiment 8:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: cake protein is pulverized, and described cake protein is rapeseed cake dregs, soybean cake dregs and peanut dregs, and the quality of rapeseed cake dregs, soybean cake dregs, peanut dregs respectively accounts for 1/3; Cross 60 mesh sieves then, obtain the cake protein powder;
2) Microwave Pretreatment:
Solid-liquid ratio by cake protein powder and phosphoric acid buffer is 1g: 30mL, chooses cake protein powder and phosphoric acid buffer, and the cake protein powder is placed the microwave reaction bottle, add phosphoric acid buffer, be put in the microwave device, 80 ℃, the following reaction of 800W 4min, take out, obtain the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 8000U/g; Place microwave device then, 60 ℃, the following reaction of 600W 30min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 50LAPU/g; Place microwave device then, 60 ℃, the following reaction of 600W 30min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Embodiment 9:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: cake protein is pulverized, crossed 30 mesh sieves then, obtain the cake protein powder; Described cake protein is a soybean cake dregs;
2) Microwave Pretreatment:
Solid-liquid ratio by cake protein powder and phosphoric acid buffer is 1g: 25mL, chooses cake protein powder and phosphoric acid buffer, and the cake protein powder is placed the microwave reaction bottle, add phosphoric acid buffer, be put in the microwave device, 60 ℃, the following reaction of 700W 3min, take out, obtain the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
Embodiment 10:
Microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, and it comprises the steps:
1) preparation of raw material: cake protein is pulverized, crossed 20 mesh sieves then, obtain the cake protein powder; Described cake protein is a soybean cake dregs;
2) Microwave Pretreatment:
Solid-liquid ratio by cake protein powder and phosphoric acid buffer is 1g: 20mL, chooses cake protein powder and phosphoric acid buffer, and the cake protein powder is placed the microwave reaction bottle, add phosphoric acid buffer, be put in the microwave device, 50 ℃, the following reaction of 600W 2min, take out, obtain the Microwave Pretreatment material;
3) microwave enzymolysis:
With Microwave Pretreatment material adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of Microwave Pretreatment material is: 50LAPU/g; Place microwave device then, 40 ℃, the following reaction of 400W 5min take out, and get microwave enzymolysis material B;
With microwave enzymolysis material B adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material B is: 500U/g; Place microwave device then, 40 ℃, the following reaction of 400W 5min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
The bound of each raw material of the present invention, interval value, and the bound of processing parameter (as temperature, time etc.), interval value can both realize the present invention, the variety of way of step 3) microwave enzymolysis can both realize the present invention, does not enumerate embodiment one by one at this.
Claims (3)
1. microwave-assisted enzymolysis oil meal protein is produced the method for active polypeptide, it is characterized in that it comprises the steps:
1) preparation of raw material: cake protein is pulverized, crossed 20～60 mesh sieves then, obtain the cake protein powder;
2) Microwave Pretreatment:
Solid-liquid ratio by cake protein powder and damping fluid is 1g: 20～30mL, chooses cake protein powder and damping fluid, and the cake protein powder is placed the microwave reaction bottle, add damping fluid, be put in the microwave device, 50～80 ℃, the following reaction of 600～800W 2～4min, take out, obtain the Microwave Pretreatment material;
3) adopt one of following 9 kinds of modes to carry out the microwave enzymolysis:
1. be 8.0 with Microwave Pretreatment material adjust pH, add Sumizyme MP, Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
2. be 7.0 with Microwave Pretreatment material adjust pH, add flavor protease, flavor protease with the ratio of Microwave Pretreatment material is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
3. be 7.0 with Microwave Pretreatment material adjust pH, add neutral protease, neutral protease with the ratio of Microwave Pretreatment material is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
4. be 8.0 with Microwave Pretreatment material adjust pH, add Sumizyme MP, Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material A is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
5. be 8.0 with Microwave Pretreatment material adjust pH, add Sumizyme MP, Sumizyme MP with the ratio of Microwave Pretreatment material is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material A;
With microwave enzymolysis material A adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material A is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
6. be 7.0 with Microwave Pretreatment material adjust pH, add flavor protease, flavor protease with the ratio of Microwave Pretreatment material is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material B;
With microwave enzymolysis material B adjust pH is 7.0, adds neutral protease, and neutral protease with the ratio of microwave enzymolysis material B is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
7. be 7.0 with Microwave Pretreatment material adjust pH, add flavor protease, flavor protease with the ratio of Microwave Pretreatment material is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material B;
With microwave enzymolysis material B adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of microwave enzymolysis material B is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
8. be 7.0 with Microwave Pretreatment material adjust pH, add neutral protease, neutral protease with the ratio of Microwave Pretreatment material is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material C;
With microwave enzymolysis material C adjust pH is 8.0, adds Sumizyme MP, and Sumizyme MP with the ratio of microwave enzymolysis material C is: 2000～8000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
9. be 7.0 with Microwave Pretreatment material adjust pH, add neutral protease, neutral protease with the ratio of Microwave Pretreatment material is: 500～2000U/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get microwave enzymolysis material C;
With microwave enzymolysis material C adjust pH is 7.0, adds flavor protease, and flavor protease with the ratio of microwave enzymolysis material C is: 50～100LAPU/g; Place microwave device then, 40～60 ℃, the following reaction of 400～600W 5～30min take out, and get enzymolysis solution;
4) the enzymolysis solution enzyme that goes out obtains active polypeptide.
2. microwave-assisted enzymolysis oil meal protein according to claim 1 is produced the method for active polypeptide, it is characterized in that; Described cake protein is any one or any two kinds mixing in rapeseed cake dregs, soybean cake dregs, the peanut dregs, and any two kinds is any proportioning when mixing.
3. microwave-assisted enzymolysis oil meal protein according to claim 1 is produced the method for active polypeptide, and it is characterized in that: described damping fluid is phosphoric acid buffer, borate buffer or tris buffer, and the pH value is 8.0.
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| CN102048026A (en) * | 2010-12-06 | 2011-05-11 | 中南林业科技大学 | Preparation method of oil-tea camellia meal protein polypeptide as additive for functional feeds |
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| CN103478403A (en) * | 2013-09-06 | 2014-01-01 | 江苏丘陵地区镇江农业科学研究所 | Peanut polypeptide and preparation method thereof |
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| CN101849607A (en) * | 2010-05-21 | 2010-10-06 | 东北农业大学 | Method for increasing content of soluble proteins of high-temperature denatured soybean meal by microwave processing |
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| CN102250998B (en) * | 2011-05-27 | 2013-09-25 | 山东省花生研究所 | Preparation method for peanut bioactive peptide |
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| CN104256741A (en) * | 2014-10-16 | 2015-01-07 | 青岛博之源生物技术有限公司 | Preparation method of black-coat peanut and highland barley breakfast powder suitable for diabetics |
| CN104531525A (en) * | 2014-12-30 | 2015-04-22 | 江苏天琦生物科技有限公司 | Production system of oil cake functional polypeptide |
| CN104480177A (en) * | 2014-12-31 | 2015-04-01 | 广西壮族自治区农业科学院农产品加工研究所 | Preparation method of cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor |
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| CN105039089B (en) * | 2015-09-08 | 2018-02-06 | 南宁荣港生物科技有限公司 | A kind of pawpaw peptide wine and preparation method thereof |
| CN107164443A (en) * | 2017-06-05 | 2017-09-15 | 深圳知本康业有限公司 | A kind of soya bean protein polypeptide and application |
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