CN114045316A - Modified cereal dietary fiber and preparation method thereof - Google Patents
Modified cereal dietary fiber and preparation method thereof Download PDFInfo
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- CN114045316A CN114045316A CN202111369316.4A CN202111369316A CN114045316A CN 114045316 A CN114045316 A CN 114045316A CN 202111369316 A CN202111369316 A CN 202111369316A CN 114045316 A CN114045316 A CN 114045316A
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 45
- 235000013339 cereals Nutrition 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000000034 method Methods 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 238000000502 dialysis Methods 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 24
- 238000001035 drying Methods 0.000 claims abstract description 15
- 108010059892 Cellulase Proteins 0.000 claims abstract description 13
- 229940106157 cellulase Drugs 0.000 claims abstract description 13
- 239000006228 supernatant Substances 0.000 claims abstract description 12
- 230000009471 action Effects 0.000 claims abstract description 8
- 238000005238 degreasing Methods 0.000 claims abstract description 8
- JBTWLSYIZRCDFO-UHFFFAOYSA-N ethyl methyl carbonate Chemical compound CCOC(=O)OC JBTWLSYIZRCDFO-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 230000001376 precipitating effect Effects 0.000 claims abstract description 8
- 238000007873 sieving Methods 0.000 claims abstract description 8
- 238000002604 ultrasonography Methods 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 244000062793 Sorghum vulgare Species 0.000 claims description 3
- 241000209140 Triticum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 235000019713 millet Nutrition 0.000 claims description 3
- 239000012716 precipitator Substances 0.000 claims description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 2
- 239000008158 vegetable oil Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 abstract description 13
- 238000012545 processing Methods 0.000 abstract description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 150000002148 esters Chemical class 0.000 description 6
- 239000012535 impurity Substances 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 1
- FIVPIPIDMRVLAY-UHFFFAOYSA-N aspergillin Natural products C1C2=CC=CC(O)C2N2C1(SS1)C(=O)N(C)C1(CO)C2=O FIVPIPIDMRVLAY-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- FIVPIPIDMRVLAY-RBJBARPLSA-N gliotoxin Chemical compound C1C2=CC=C[C@H](O)[C@H]2N2[C@]1(SS1)C(=O)N(C)[C@@]1(CO)C2=O FIVPIPIDMRVLAY-RBJBARPLSA-N 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000003871 intestinal function Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Mycology (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a modified cereal dietary fiber and a preparation method thereof, belonging to the technical field of cereal processing and comprising the following steps: s1, crushing and sieving the grain raw materials, adding an ester solvent, and carrying out degreasing treatment; s2, adding the degreased raw material of S1, adjusting the pH value to 5-6, and adding cellulase for enzymolysis under an ultrasonic condition to obtain an enzymolysis liquid; s3, centrifuging the S2 enzymolysis liquid, and taking supernate; s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid; s5, concentrating the trapped liquid S4, precipitating with ethanol, and drying to prepare modified cereal dietary fiber; the method is simple and environment-friendly, and the obtained product has high yield and good purity and is suitable for popularization and application.
Description
Technical Field
The invention belongs to the technical field of grain processing, and particularly relates to modified grain dietary fiber and a preparation method thereof.
Background
The grains are important sources of nutrition and energy of people, contain a large amount of dietary fibers, and can be divided into insoluble dietary fibers and water-soluble dietary fibers according to solubility, wherein the water-soluble dietary fibers are easy to absorb by a human body due to good water solubility, so that the water-soluble dietary fibers have a great promotion effect on the human body, for example, the water-soluble dietary fibers can improve the intestinal function, increase satiety, reduce blood fat and cholesterol, and can wrap toxic substances such as ammonia and aspergillin to prevent the toxic substances from being absorbed by the intestinal tract and being taken out of the body, so that the water-soluble dietary fibers have the effect of expelling toxins. In view of the above-mentioned effects of water-soluble dietary fibers, they are gradually extracted separately and added to specific health foods in order to better utilize their health effects.
The existing method for preparing the water-soluble dietary fiber can be roughly divided into a chemical method, a physical method and a biological method, wherein most of the chemical methods use acid-base treatment, and the subsequent treatment steps are complex and the product loss is large; physical methods such as cooking, although the conditions are mild compared with chemical methods using acid-base treatment agents, the production equipment is greatly limited; the biological method has mild conditions, but is not easy to expand the production; therefore, research on how to simply and efficiently prepare water-soluble dietary fiber should be continued, which contributes to further expansion of its application value.
Disclosure of Invention
Aiming at the technical problems, the invention provides a modified cereal dietary fiber and a preparation method thereof.
The invention is realized by the following technical scheme.
The first purpose of the invention is to provide a preparation method of modified cereal dietary fiber, which comprises the following steps:
s1, crushing and sieving the grain raw materials, adding an ester solvent, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 5-6, and adding cellulase for enzymolysis under the ultrasonic condition to obtain an enzymolysis liquid;
s3, centrifuging the S2 enzymolysis liquid, and taking supernate;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid;
s5, concentrating the trapped liquid S4, precipitating with ethanol, and drying to prepare the modified cereal dietary fiber.
Preferably, in S1, the ester solvent is ethanol or vegetable oil.
Preferably, in S1, the grains are one or more of rice, wheat, millet and soybean, and the crushed raw materials are sieved by a 50-70 mesh sieve.
Preferably, in S2, the pH is adjusted with citric acid.
Preferably, in S2, the ultrasonic condition is 100-300W, and the ultrasonic is performed once every 20-40 min, 15-30 min each time.
Preferably, in S3, the centrifugation condition is centrifugation for 15-30 min at 2000-3000 r/min, and then centrifugation for 10-20 min at 4000-6000 r/min.
Preferably, in the S4, in the dialysis process, a dialysis bag with the molecular weight cutoff of 1-3 KDa is selected, and deionized water is used for dialysis for 1-3 hours.
Preferably, in S4, the ultrasonic condition is that the power is 50-150W and the ultrasonic time is 15-30 min.
Preferably, in S5, the concentration is carried out under the conditions that the pressure is 0.04-0.06 MPa and the temperature is 40-70 ℃, the volume is concentrated to 1/2-2/3 of the original volume, the alcohol precipitation is carried out by adopting ethanol as a precipitator, and the drying is carried out under the conditions that the temperature is 80-100 ℃ and the wind speed is 1-3 m/S, until the water content is lower than 10%.
The second purpose of the invention is to provide the modified cereal dietary fiber prepared by the preparation method.
Compared with the prior art, the invention has the following beneficial effects:
the method comprises the steps of firstly soaking crushed and sieved grain raw materials by using an ester solvent to dissolve out ester substances in the grains and remove the ester substances, then adding cellulase to carry out enzymolysis on the raw materials after the ester is removed, wherein the enzymolysis process is accompanied with ultrasonic treatment, so that the enzymolysis process can be accelerated, more importantly, the ultrasonic treatment can destroy the effects among a crystalline region of cellulose, hemicellulose and lignin, the cellulase can be more fully contacted with an enzymolysis substrate, the enzymolysis process is further accelerated, and meanwhile, the content and the quality of an enzymolysis product are improved; then the enzymolysis liquid is centrifuged to obtain supernatant, the supernatant is dialyzed, ultrasonic treatment is carried out in the dialysis process, the dialysis speed can be accelerated, meanwhile, impurities in the enzymolysis liquid can be fully shaken out from the enzymolysis product molecules and then removed through dialysis, the traditional method directly carries out concentration and drying after centrifugal treatment, a large amount of alcohol is used for precipitation after concentration, the impurities can be brought out by the product in the alcohol precipitation process, the centrifugal treatment and dialysis treatment are carried out in the invention, and the ultrasonic treatment is carried out in the dialysis process, so that the impurities can be removed in advance, and the purity and the yield of the product are improved in the process. The method is simple and environment-friendly, and the obtained product has high yield and good purity and is suitable for popularization and application.
Detailed Description
In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following examples and data are provided for further illustration, but the examples are not intended to limit the present invention. The starting materials and reagents used in the following examples are commercially available unless otherwise specified, and the test methods and detection methods used therein are conventional unless otherwise specified.
The invention provides a preparation method of modified cereal dietary fiber, which comprises the following steps:
s1, crushing and sieving the grain raw materials, adding an ester solvent, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 5-6, and adding cellulase for enzymolysis under the ultrasonic condition to obtain an enzymolysis liquid; cellulase is purchased from Shanghai leaf Biotech, Inc., cat # S10041; the addition amount is 4-6 u/g.
S3, centrifuging the S2 enzymolysis liquid, and taking supernate;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid;
s5, concentrating the trapped liquid S4, precipitating with ethanol, and drying to prepare the modified cereal dietary fiber.
The following specific examples 1 to 4 specifically describe the contents of the above invention.
Example 1
A preparation method of modified cereal dietary fiber comprises the following steps:
s1, crushing the rice raw material, sieving the crushed rice raw material by a 50-mesh sieve, adding ethanol, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 5 by using citric acid, and adding cellulase for enzymolysis (4u/g) under the ultrasonic condition to obtain an enzymolysis liquid; the ultrasonic condition is 100W, and the ultrasonic is carried out once every 20min, 15min each time;
s3, centrifuging the S2 enzymolysis liquid, and taking supernate; the centrifugation condition is that the centrifugation is carried out for 15min at the rotating speed of 2000r/min, and then the centrifugation is carried out for 10min at the rotating speed of 4000 r/min;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid; in the dialysis process, a dialysis bag with the molecular weight cutoff of 1KDa is selected, and deionized water is used for dialysis for 1 h; the ultrasonic condition is that the power is 50W, and the ultrasonic time is 15 min;
s5, concentrating the trapped liquid S4, precipitating with 95% ethanol, and drying, wherein the concentration condition is that the trapped liquid is concentrated to 1/2 of the original volume under the pressure of 0.04MPa and the temperature of 40 ℃, and the drying condition is that the trapped liquid is dried to the water content of less than 10% under the conditions of the temperature of 80 ℃ and the wind speed of 1m/S, so that the modified cereal dietary fiber can be prepared.
Example 2
A preparation method of modified cereal dietary fiber comprises the following steps:
s1, crushing the wheat raw material, sieving with a 70-mesh sieve, adding soybean oil, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 6 by using citric acid, and adding cellulase for enzymolysis (6u/g) under the ultrasonic condition to obtain an enzymolysis liquid; the ultrasonic condition is 300W of power, and ultrasonic is carried out once every 40min, 30min each time;
s3, centrifuging the S2 enzymolysis liquid, and taking supernate; centrifuging at 3000r/min for 30min, and centrifuging at 6000r/min for 20 min;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid; in the dialysis process, a dialysis bag with the molecular weight cutoff of 3KDa is selected, and deionized water is used for dialysis for 1-3 h; the ultrasonic condition is that the power is 150W, and the ultrasonic time is 30 min;
s5, concentrating the trapped liquid S4, precipitating with 95% ethanol, and drying, wherein the concentration condition is that the trapped liquid is concentrated to 2/3 of the original volume under the pressure of 0.06MPa and the temperature of 70 ℃, and the drying condition is that the trapped liquid is dried to the water content of less than 10% under the conditions of the temperature of 100 ℃ and the wind speed of 3m/S, so that the modified cereal dietary fiber can be prepared.
Example 3
A preparation method of modified cereal dietary fiber comprises the following steps:
s1, crushing the soybean raw material, sieving the crushed soybean raw material with a 50-mesh sieve, adding ethanol, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 6 by using citric acid, and adding cellulase for enzymolysis (4u/g) under the ultrasonic condition to obtain an enzymolysis liquid; the ultrasonic condition is 200W of power, and ultrasonic is carried out once every 30min, 20min each time;
s3, centrifuging the S2 enzymolysis liquid, and taking supernate; centrifuging at 2500r/min for 20min, and centrifuging at 5000r/min for 15 min;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid; in the dialysis process, a dialysis bag with the molecular weight cutoff of 2KDa is selected, and deionized water is used for dialysis for 2 hours; the ultrasonic condition is that the power is 100W, and the ultrasonic time is 20 min;
s5, concentrating the trapped liquid S4, precipitating with 95% ethanol, and drying, wherein the concentration condition is that the trapped liquid is concentrated to 2/3 of the original volume under the pressure of 0.05MPa and the temperature of 50 ℃, and the drying condition is that the trapped liquid is dried to the water content of less than 10% under the conditions of the temperature of 900 ℃ and the wind speed of 2m/S, so that the modified cereal dietary fiber can be prepared.
Example 4
A preparation method of modified cereal dietary fiber comprises the following steps:
s1, crushing the millet raw material, sieving with a 50-mesh sieve, adding ethanol, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 6 by using citric acid, and adding cellulase for enzymolysis (6u/g) under the ultrasonic condition to obtain an enzymolysis liquid; the ultrasonic condition is that the power is 250W, and ultrasonic is carried out once every 35min, and each time is 25 min;
s3, centrifuging the S2 enzymolysis liquid, and taking supernate; centrifuging at 3000r/min for 25min, and centrifuging at 5500r/min for 20 min;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid; in the dialysis process, a dialysis bag with the molecular weight cutoff of 3KDa is selected, and deionized water is used for dialysis for 2.5 hours; the ultrasonic condition is that the power is 120W, and the ultrasonic time is 25 min;
s5, concentrating the trapped liquid S4, precipitating with 95% ethanol, and drying, wherein the concentration condition is that the trapped liquid is concentrated to 2/3 of the original volume under the pressure of 0.06MPa and the temperature of 60 ℃, and the drying condition is that the trapped liquid is dried to the water content of less than 10% under the conditions of the temperature of 100 ℃ and the wind speed of 3m/S, so that the modified cereal dietary fiber can be prepared.
Comparative example 1
The method was the same as in example 1, except that there was no ultrasonic treatment in S2.
Comparative example 2
The method was the same as in example 1, except that there was no ultrasonic treatment in S4.
The results of the tests on the dietary fibers obtained in examples 1 to 4 are shown in Table 1:
TABLE 1 dietary fiber yields obtained in examples 1-4 and comparative examples 1-2
The purity results of the dietary fibers obtained in examples 1 to 4 were measured and shown in Table 2:
TABLE 2 purity of dietary fiber obtained in examples 1 to 4 and comparative examples 1 to 2
As can be seen from table 1, comparative example 1 does not use ultrasonic treatment in the enzymolysis process, and comparative example 2 does not use ultrasonic treatment in the product dialysis purification process, and compared with comparative example 1 and comparative example 2, the dietary fibers prepared in examples 1 to 4 of the present application have higher yields, which indicates that the present application respectively uses ultrasonic treatment in the enzymolysis process and the dialysis process, and can improve the yield of the product; the table 2 also shows that the dietary fibers prepared in the embodiments 1 to 4 of the present invention have high purity, which indicates that the method of the present invention is feasible and has a better effect, because the present invention firstly uses an ester solvent to soak the crushed and sieved cereal raw materials to dissolve out ester substances in the cereal and remove the ester substances, and then adds cellulase to carry out enzymolysis on the raw materials from which the ester substances are removed, and the enzymolysis process is accompanied with ultrasonic treatment, which not only can accelerate the enzymolysis process, but also more importantly destroys the effects among the crystalline region of the cellulose, hemicellulose and lignin, so that the cellulase can be more fully contacted with an enzymolysis substrate, further accelerates the enzymolysis process, and simultaneously improves the content and quality of enzymolysis products; then the enzymolysis liquid is centrifuged to obtain supernatant, the supernatant is dialyzed, the dialysis speed can be accelerated along with ultrasonic in the dialysis process, meanwhile, impurities in the enzymolysis liquid can be shaken out from the enzymolysis product molecules and removed through dialysis, the traditional step of carrying out aftertreatment by using a large amount of ethanol is simplified in the process, the dialyzed product is directly concentrated and dried, the alcohol consumption is reduced, the operation steps are simplified, and the operation time is saved. In conclusion, the method is simple and environment-friendly, and the obtained product has high yield and good purity and is suitable for popularization and application.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, it is intended that such changes and modifications be included within the scope of the appended claims and their equivalents.
Claims (10)
1. A preparation method of modified cereal dietary fiber is characterized by comprising the following steps:
s1, crushing and sieving the grain raw materials, adding an ester solvent, and carrying out degreasing treatment;
s2, adjusting the pH value of the degreased raw material of S1 to 5-6, and adding cellulase for enzymolysis under the ultrasonic condition to obtain an enzymolysis liquid;
s3, centrifuging the S2 enzymolysis liquid, and taking supernate;
s4, subjecting the supernatant of S3 to dialysis treatment under the action of ultrasound, and taking trapped fluid;
s5, concentrating the trapped liquid S4, precipitating with ethanol, and drying to prepare the modified cereal dietary fiber.
2. The method according to claim 1, wherein the ester solvent is ethanol or vegetable oil at S1.
3. The preparation method of the modified cereal dietary fiber as claimed in claim 1, wherein in S1, the cereal is one or more of rice, wheat, millet and soybean, and the crushed raw materials are sieved by a 50-70 mesh sieve.
4. The method for producing a modified cereal dietary fiber according to claim 1, wherein in S2, the pH is adjusted with citric acid.
5. The method for preparing modified cereal dietary fiber according to claim 1, wherein in S2, the ultrasonic condition is 100-300W, and the ultrasonic is performed every 20-40 min for 15-30 min.
6. The preparation method of the modified cereal dietary fiber as claimed in claim 1, wherein in S3, the centrifugation is carried out for 15-30 min at 2000-3000 r/min and then for 10-20 min at 4000-6000 r/min.
7. The method for preparing modified cereal dietary fiber according to claim 1, wherein in the dialysis process of S4, a dialysis bag with a molecular weight cut-off of 1-3 KDa is selected, and deionized water is dialyzed for 1-3 h.
8. The preparation method of the modified cereal dietary fiber as claimed in claim 1, wherein in S4, the ultrasonic condition is 50-150W of power and the ultrasonic time is 15-30 min.
9. The method for preparing modified cereal dietary fiber according to claim 1, wherein in S5, the concentration is performed under the conditions of 0.04-0.06 MPa of pressure and 40-70 ℃ until the volume is 1/2-2/3 of the original volume, the alcohol precipitation is performed by using ethanol as a precipitator, and the drying is performed under the conditions of 80-100 ℃ of temperature and 1-3 m/S of wind speed until the water content is less than 10%.
10. The modified cereal dietary fiber prepared by the preparation method according to any one of claims 1 to 9.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114521657A (en) * | 2022-03-01 | 2022-05-24 | 珠海津之敦医药科技有限公司 | Cereal beta-glucan co-evaporation compound and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101545837A (en) * | 2009-04-30 | 2009-09-30 | 广西师范大学 | A method for ultrasound dialyzing, a special set for the method and the application of the method |
| CN104473188A (en) * | 2015-01-13 | 2015-04-01 | 中南林业科技大学 | Method for extracting water-soluble dietary fibers from rice bran |
| CN105361187A (en) * | 2015-09-28 | 2016-03-02 | 华中农业大学 | Modified cereal dietary fiber and preparation method thereof |
| CN109170429A (en) * | 2018-09-06 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of modified bean dregs dietary fiber beverage |
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2021
- 2021-11-18 CN CN202111369316.4A patent/CN114045316A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101545837A (en) * | 2009-04-30 | 2009-09-30 | 广西师范大学 | A method for ultrasound dialyzing, a special set for the method and the application of the method |
| CN104473188A (en) * | 2015-01-13 | 2015-04-01 | 中南林业科技大学 | Method for extracting water-soluble dietary fibers from rice bran |
| CN105361187A (en) * | 2015-09-28 | 2016-03-02 | 华中农业大学 | Modified cereal dietary fiber and preparation method thereof |
| CN109170429A (en) * | 2018-09-06 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of modified bean dregs dietary fiber beverage |
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| CN114521657A (en) * | 2022-03-01 | 2022-05-24 | 珠海津之敦医药科技有限公司 | Cereal beta-glucan co-evaporation compound and preparation method and application thereof |
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