CN112940146A - Preparation method of tremella polysaccharide - Google Patents
Preparation method of tremella polysaccharide Download PDFInfo
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- CN112940146A CN112940146A CN202110448568.XA CN202110448568A CN112940146A CN 112940146 A CN112940146 A CN 112940146A CN 202110448568 A CN202110448568 A CN 202110448568A CN 112940146 A CN112940146 A CN 112940146A
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- 241001506047 Tremella Species 0.000 title claims abstract description 90
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 70
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 150000004676 glycans Chemical class 0.000 title claims abstract 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 238000002386 leaching Methods 0.000 claims abstract description 31
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 19
- 239000002244 precipitate Substances 0.000 claims abstract description 18
- 238000001471 micro-filtration Methods 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 238000001728 nano-filtration Methods 0.000 claims abstract description 10
- 239000000919 ceramic Substances 0.000 claims abstract description 9
- 238000001816 cooling Methods 0.000 claims abstract description 9
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 238000007873 sieving Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 23
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 239000000047 product Substances 0.000 abstract description 6
- 230000009467 reduction Effects 0.000 abstract description 3
- 230000006378 damage Effects 0.000 abstract description 2
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 150000004804 polysaccharides Chemical class 0.000 description 55
- 238000000034 method Methods 0.000 description 20
- 238000000605 extraction Methods 0.000 description 19
- 239000000126 substance Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 238000001035 drying Methods 0.000 description 6
- 239000000706 filtrate Substances 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 238000000874 microwave-assisted extraction Methods 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 108010059820 Polygalacturonase Proteins 0.000 description 2
- 229920001284 acidic polysaccharide Polymers 0.000 description 2
- 150000004805 acidic polysaccharides Chemical class 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 108010093305 exopolygalacturonase Proteins 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002137 ultrasound extraction Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000002925 chemical effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
A preparation method of tremella polysaccharide comprises selecting dried high-quality tremella, pulverizing with a pulverizer, and sieving with a 40-mesh sieve to obtain dried tremella powder; adding 10-20 times of water by weight into the powder obtained in the step one, and circularly leaching with hot water to obtain a leaching solution; carrying out microfiltration treatment on the leaching liquor obtained in the step two through a ceramic membrane, and carrying out reduced pressure concentration on the obtained microfiltration permeating liquor to obtain thick paste; fourthly, cooling the thick paste obtained in the third step to 2-4 ℃, adding an ethanol water solution, sequentially stirring, centrifuging and standing, and removing supernate to obtain polysaccharide precipitate; fifthly, adding ethyl acetate and water into the polysaccharide precipitate obtained in the step IV, extracting, collecting water, performing nanofiltration concentration, and freeze-drying the concentrated solution to obtain the tremella polysaccharide. The preparation method provided by the invention overcomes a series of product problems of destruction of the space structure of the tremella polysaccharide, reduction of the molecular weight of the tremella polysaccharide, large capital investment, high equipment cost, high noise and electromagnetic wave pollution of the product and the like in other traditional tremella polysaccharide preparation methods.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a preparation method of tremella polysaccharide.
Background
Tremella is a traditional edible fungus medicine in China and is a name of 'crown of fungus'. As recorded in the materia medica-Zhongxin, tremella has the effects of moistening lung and nourishing yin. Modern pharmacological research proves that the content of tremella polysaccharide in tremella is 60-70%. The tremella polysaccharide is an effective component of tremella, has high physiological activity, can induce an organism to generate antibodies, has various pharmacological effects such as free radical removal, tumor resistance, organism immunity improvement and the like, has an auxiliary treatment effect on various difficult and complicated diseases such as hypertension, diabetes and the like, and shows good development prospects. With the progress of medical technology, the tremella polysaccharide product is not only applied to health-care food, but also applied to the fields of cosmetics and medical products. The research on tremella polysaccharide in China is relatively late, and at present, cosmetics are mainly used, and the medicinal value of tremella polysaccharide is not well utilized. The main reasons are that the extraction yield of the tremella polysaccharide is not high, the purity of the extract is not enough, and the use standard of medical supplies cannot be met. In addition, the extraction process of the tremella polysaccharide is complex, the cost is high, so the tremella polysaccharide is very expensive and is difficult to meet the consumption level of most people.
At present, the preparation method of tremella polysaccharide which can be applied industrially is still in the laboratory research stage, and people often extract and purify tremella polysaccharide in a laboratory by utilizing the characteristic that tremella polysaccharide is easy to dissolve in water and difficult to dissolve in an organic solvent. The extraction method of tremella polysaccharide mainly comprises a water extraction method, an alkali extraction method, an enzymolysis method, an ultrasonic-assisted extraction method, a microwave-assisted extraction method and the like which take tremella fruiting bodies as raw materials for extraction. The proportion of the acidic polysaccharide in the tremella polysaccharide is higher, so that the extraction efficiency of the acidic polysaccharide can be obviously improved by adopting an alkali extraction method; the enzymolysis method mainly utilizes enzymes (such as pectinase, cellulase, protease, etc.) to remove substances which hinder extraction, thereby achieving the purpose of improving the extraction efficiency; the ultrasonic method mainly utilizes the ultrasonic action to form mechanical vibration of medium particles in the tremella cells, the mechanical action, the cavitation action and the like are acted between the tremella cells and water, and mechanical effect, thermal effect, optical effect, electrical effect, chemical effect and the like are generated, so that the extraction efficiency of tremella polysaccharide is improved; the microwave-assisted extraction method mainly causes polar substances in cells to absorb microwaves, generates a large amount of heat, gasifies liquid water to enable the cells to form holes and even expand and break, reduces water in the cells and cell walls, enables external solvents to enter the cells more easily, and promotes dissolution and diffusion of cell contents. Then, the current tremella polysaccharide extraction methods have certain defects and shortcomings. For example, the alkali extraction method can destroy the spatial structure of the tremella polysaccharide, thereby changing the biological activity and function of the tremella polysaccharide. Cellulase, pectinase and the like in the enzymolysis method can cut off certain glycosidic bonds of the extracted tremella polysaccharide, so that the molecular weight of the tremella polysaccharide is reduced. The ultrasonic and microwave assisted extraction method has the disadvantages of large capital investment, high equipment cost, and high noise and electromagnetic wave pollution.
Therefore, the problem to be solved urgently in the industry at present is to explore an extraction process with high extraction rate, low investment and small environmental pollution.
Disclosure of Invention
The inventor finds that the tremella polysaccharide is prepared by taking mechanically crushed tremella as a raw material and adopting a water extraction and alcohol precipitation method, and the tremella polysaccharide is finally prepared by processes of purification, nanofiltration concentration, freeze drying and the like, so that the tremella polysaccharide preparation method capable of being applied industrially is provided. The preparation method provided by the invention overcomes a series of product problems of destruction of the space structure of the tremella polysaccharide, reduction of the molecular weight of the tremella polysaccharide, large capital investment, high equipment cost, high noise and electromagnetic wave pollution of the product and the like in other traditional tremella polysaccharide preparation methods. The method of the invention improves the extraction rate of the tremella polysaccharide to more than 38 percent, and the extract has stronger free radical scavenging and reducing abilities.
The method comprises the following specific steps:
step 1, selecting high-quality dried tremella, crushing by a crusher, and sieving by a 40-mesh sieve to obtain dried tremella powder;
step 2, adding 10-20 times of water by weight into the powder obtained in the step 1, and circularly leaching with hot water to obtain a leaching solution;
step 3, performing microfiltration treatment on the leaching liquor obtained in the step 2 through a ceramic membrane, and performing reduced pressure concentration on the obtained microfiltration permeate to obtain thick paste;
step 4, cooling the thick paste obtained in the step 3 to 2-4 ℃, adding an ethanol water solution, stirring, centrifuging, standing, and removing supernatant to obtain polysaccharide precipitate;
and 5, adding ethyl acetate and water into the polysaccharide precipitate obtained in the step 4, extracting, collecting water, carrying out nanofiltration concentration, and freeze-drying the concentrated solution to obtain the polysaccharide.
Preferably, the leaching time in the step 2 is 50-70 min, and the leaching water temperature is controlled at 90-100 ℃.
Preferably, in the step 3, the vacuum degree of the reduced pressure concentration tank is 0.07-0.09 MPa, the concentration temperature is controlled at 55-75 ℃, and the concentration time is controlled at 1-3 h.
Preferably, the concentration of the ethanol aqueous solution in the step 4 is controlled to be 85-95%, and the volume ratio of the added ethanol aqueous solution to the thick paste obtained in the step 3 is 6: 1-5: 1.
Compared with the prior art, the invention has the beneficial results that: according to the preparation process of the tremella polysaccharide, the tremella fine powder is crushed, extracted in a hot water circulation mode, precipitated in alcohol, purified and then subjected to freeze drying technology to obtain a target product; the tremella polysaccharide extraction process provided by the invention adopts a water extraction and alcohol precipitation method to prepare tremella polysaccharide, and provides a tremella polysaccharide preparation method which can be applied industrially. The preparation method provided by the invention overcomes a plurality of bottleneck problems existing in other traditional tremella polysaccharide preparation methods, has the advantages of low extraction cost, high extraction efficiency, reduction of environmental pollution and the like, can effectively improve the extraction rate of tremella polysaccharide, and is suitable for large-scale industrial production.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
step 1: selecting high-quality dried tremella, crushing by using a crusher, additionally arranging a 40-mesh sieve at the outlet of the crusher, and sieving to obtain dried tremella powder. Naturally, fresh tremella can be selected in this step, the fresh tremella is placed in a drying oven at 60-80 ℃, and the crushing process can be repeated when the water content is less than 10%.
Step 2: and (2) adding 10 times of hot water with the temperature of 90 ℃ in weight into the powder obtained in the step (1), circularly leaching for 50min in a heat-preservation environment, and filtering out impurities to obtain a leaching solution.
And step 3: and (3) performing microfiltration treatment on the leaching liquor obtained in the step (2) through a ceramic membrane, and performing reduced pressure concentration on the obtained micro-filtrate at the vacuum degree of 0.07-0.09 MPa, wherein the concentration temperature is 55-75 ℃, and the concentration time is 1h, so as to obtain thick paste.
And 4, step 4: cooling the thick paste obtained in the step 3 to 2-4 ℃, adding 85% ethanol water solution, stirring, centrifuging for 15min, standing for 2h, and removing supernatant, namely polysaccharide precipitate, wherein the volume ratio of the ethanol solution to the thick paste is 6: 1.
And 5: adding ethyl acetate and water into the precipitate of the polysaccharide in the step 4, extracting, and collecting a water phase; and (4) carrying out nanofiltration concentration on the obtained aqueous solution, and freeze-drying the concentrated solution. The obtained substance is the tremella polysaccharide crude sugar.
Example 2:
step 1: selecting high-quality dried tremella, crushing by using a crusher, additionally arranging a 40-mesh sieve at the outlet of the crusher, and sieving to obtain dried tremella powder. Naturally, fresh tremella can be selected in this step, the fresh tremella is placed in a drying oven at 60-80 ℃, and the crushing process can be repeated when the water content is less than 10%.
Step 2: and (2) adding 20 times of hot water with the temperature of 100 ℃ into the powder obtained in the step (1), circularly leaching for 50min in a heat preservation environment, and filtering out impurities to obtain a leaching solution.
And step 3: and (3) performing microfiltration treatment on the leaching liquor obtained in the step (2) through a ceramic membrane, and performing reduced pressure concentration on the obtained micro-filtrate at the vacuum degree of 0.07-0.09 MPa, wherein the concentration temperature is 55-75 ℃, and the concentration time is 3 hours, so as to obtain thick paste.
And 4, step 4: cooling the thick paste obtained in the step 3 to 2-4 ℃, adding 95% ethanol water solution and stirring, wherein the volume ratio of the ethanol solution to the thick paste is 5:1, centrifuging for 15min, standing for 2h, and removing supernatant, namely polysaccharide precipitate.
And 5: adding ethyl acetate and water into the precipitate of the polysaccharide in the step 4, extracting, and collecting a water phase; and (4) carrying out nanofiltration concentration on the obtained aqueous solution, and freeze-drying the concentrated solution. The obtained substance is the tremella polysaccharide crude sugar.
Example 3:
step 1: selecting high-quality dried tremella, crushing by using a crusher, additionally arranging a 40-mesh sieve at the outlet of the crusher, and sieving to obtain dried tremella powder. Naturally, fresh tremella can be selected in this step, the fresh tremella is placed in a drying oven at 60-80 ℃, and the crushing process can be repeated when the water content is less than 10%.
Step 2: and (2) adding 20 times of hot water with the temperature of 95 ℃ into the powder obtained in the step (1), circularly leaching for 50min in a heat-preservation environment, and filtering out impurities to obtain a leaching solution.
And step 3: and (3) performing microfiltration treatment on the leaching liquor obtained in the step (2) through a ceramic membrane, and performing reduced pressure concentration on the obtained micro-filtrate at the vacuum degree of 0.07-0.09 MPa, wherein the concentration temperature is 55-75 ℃, and the concentration time is 2 hours, so as to obtain thick paste.
And 4, step 4: cooling the thick paste obtained in the step 3 to 2-4 ℃, adding 90% ethanol water solution and stirring, wherein the volume ratio of the ethanol solution to the thick paste is 5.5:1, centrifuging for 15min, standing for 2h, and removing supernatant, namely polysaccharide precipitate.
And 5: adding ethyl acetate and water into the precipitate of the polysaccharide in the step 4, extracting, and collecting a water phase; and (4) carrying out nanofiltration concentration on the obtained aqueous solution, and freeze-drying the concentrated solution. The obtained substance is the tremella polysaccharide crude sugar.
Example 4:
step 1: selecting high-quality dried tremella, crushing by using a crusher, additionally arranging a 40-mesh sieve at the outlet of the crusher, and sieving to obtain dried tremella powder. Naturally, fresh tremella can be selected in this step, the fresh tremella is placed in a drying oven at 60-80 ℃, and the crushing process can be repeated when the water content is less than 10%.
Step 2: and (2) adding 20 times of hot water with the temperature of 95 ℃ into the powder obtained in the step (1), circularly leaching for 70min in a heat-preservation environment, and filtering out impurities to obtain a leaching solution.
And step 3: and (3) performing microfiltration treatment on the leaching liquor obtained in the step (2) through a ceramic membrane, and performing reduced pressure concentration on the obtained micro-filtrate at the vacuum degree of 0.07-0.09 MPa, wherein the concentration temperature is 55-75 ℃, and the concentration time is 2 hours, so as to obtain thick paste.
And 4, step 4: cooling the thick paste obtained in the step 3 to 2-4 ℃, adding 95% ethanol water solution and stirring, wherein the volume ratio of the ethanol solution to the thick paste is 5:1, centrifuging for 15min, standing for 2h, and removing supernatant, namely polysaccharide precipitate.
And 5: adding ethyl acetate and water into the precipitate of the polysaccharide in the step 4, extracting, and collecting a water phase; and (4) carrying out nanofiltration concentration on the obtained aqueous solution, and freeze-drying the concentrated solution. The obtained substance is the tremella polysaccharide crude sugar.
Example 5:
step 1: selecting high-quality dried tremella, crushing by using a crusher, additionally arranging a 40-mesh sieve at the outlet of the crusher, and sieving to obtain dried tremella powder. Naturally, fresh tremella can be selected in this step, the fresh tremella is placed in a drying oven at 60-80 ℃, and the crushing process can be repeated when the water content is less than 10%.
Step 2: and (2) adding 20 times of hot water with the temperature of 100 ℃ into the powder obtained in the step (1), circularly leaching for 50min in a heat preservation environment, and filtering out impurities to obtain a leaching solution.
And step 3: and (3) performing microfiltration treatment on the leaching liquor obtained in the step (2) through a ceramic membrane, and performing reduced pressure concentration on the obtained micro-filtrate at the vacuum degree of 0.07-0.09 MPa, wherein the concentration temperature is 55-75 ℃, and the concentration time is 3 hours, so as to obtain thick paste.
And 4, step 4: cooling the thick paste obtained in the step 3 to 2-4 ℃, adding 95% ethanol water solution and stirring, wherein the volume ratio of the ethanol solution to the thick paste is 5:1, centrifuging for 15min, standing for 2h, and removing supernatant, namely polysaccharide precipitate.
And 5: adding ethyl acetate and water into the precipitate of the polysaccharide in the step 4, extracting, and collecting a water phase; and (4) carrying out nanofiltration concentration on the obtained aqueous solution, and freeze-drying the concentrated solution. The obtained substance is the tremella polysaccharide crude sugar.
Example 6:
step 1: selecting high-quality dried tremella, crushing by using a crusher, additionally arranging a 40-mesh sieve at the outlet of the crusher, and sieving to obtain dried tremella powder. Naturally, fresh tremella can be selected in this step, the fresh tremella is placed in a drying oven at 60-80 ℃, and the crushing process can be repeated when the water content is less than 10%.
Step 2: and (2) adding 20 times of hot water with the temperature of 100 ℃ into the powder obtained in the step (1), circularly leaching for 50min in a heat preservation environment, and filtering out impurities to obtain a leaching solution.
And step 3: and (3) performing microfiltration treatment on the leaching liquor obtained in the step (2) through a ceramic membrane, and performing reduced pressure concentration on the obtained micro-filtrate at the vacuum degree of 0.07-0.09 MPa, wherein the concentration temperature is 55-75 ℃, and the concentration time is 2 hours, so as to obtain thick paste.
And 4, step 4: cooling the thick paste obtained in the step 3 to 2-4 ℃, adding 90% ethanol water solution and stirring, wherein the volume ratio of the ethanol solution to the thick paste is 5:1, centrifuging for 15min, standing for 2h, and removing supernatant, namely polysaccharide precipitate.
And 5: adding ethyl acetate and water into the precipitate of the polysaccharide in the step 4, extracting, and collecting a water phase; and (4) carrying out nanofiltration concentration on the obtained aqueous solution, and freeze-drying the concentrated solution. The obtained substance is the tremella polysaccharide crude sugar.
Claims (4)
1. The preparation method of the tremella polysaccharide is characterized by comprising the following steps:
firstly, selecting high-quality dried tremella, crushing by a crusher, and sieving by a 40-mesh sieve to obtain dried tremella powder;
adding 10-20 times of water by weight into the powder obtained in the step one, and circularly leaching with hot water to obtain a leaching solution;
carrying out microfiltration treatment on the leaching liquor obtained in the step two through a ceramic membrane, and carrying out reduced pressure concentration on the obtained microfiltration permeating liquor to obtain thick paste;
fourthly, cooling the thick paste obtained in the third step to 2-4 ℃, adding an ethanol water solution, sequentially stirring, centrifuging and standing, and removing supernate to obtain polysaccharide precipitate;
fifthly, adding ethyl acetate and water into the polysaccharide precipitate obtained in the step IV, extracting, collecting water, performing nanofiltration concentration, and freeze-drying the concentrated solution to obtain the tremella polysaccharide.
2. The preparation method of the tremella polysaccharide as claimed in claim 1, wherein the time for leaching in the second step is 50-70 min, and the temperature of the leaching water is controlled to be 90-100 ℃.
3. The preparation method of the tremella polysaccharide as claimed in claim 2, wherein the vacuum degree of the vacuum concentration tank in the third step is 0.07-0.09 MPa, the concentration temperature is controlled at 55-75 ℃, and the concentration time is controlled at 1-3 hours.
4. The preparation method of the tremella polysaccharide as claimed in claim 3, wherein the concentration of the ethanol aqueous solution in the step (iv) is controlled to be 85-95%, and the volume ratio of the added ethanol aqueous solution to the thick paste obtained in the step (iii) is 6: 1-5: 1.
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| CN116462778A (en) * | 2023-04-17 | 2023-07-21 | 浙江天草生物科技股份有限公司 | Extraction method of tremella polysaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116462778A (en) * | 2023-04-17 | 2023-07-21 | 浙江天草生物科技股份有限公司 | Extraction method of tremella polysaccharide |
| CN116462778B (en) * | 2023-04-17 | 2024-02-23 | 浙江天草生物科技股份有限公司 | Extraction method of tremella polysaccharide |
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