CN105418781A - Ultrasonic extraction method of ganoderan - Google Patents
Ultrasonic extraction method of ganoderan Download PDFInfo
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- CN105418781A CN105418781A CN201510822880.5A CN201510822880A CN105418781A CN 105418781 A CN105418781 A CN 105418781A CN 201510822880 A CN201510822880 A CN 201510822880A CN 105418781 A CN105418781 A CN 105418781A
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Abstract
The invention discloses an ultrasonic extraction method of ganoderan. The method comprises the following steps: 1, pre-treatment of raw materials; 2, degreasing; 3, coarse extraction; 4, ultrasonic alcohol precipitation; 5, deproteinization and decoloration; 6, ultrafiltration and condensation; 7, purification. By means of the ultrasonic extraction method, activity of the ganoderan is retained to the greatest extent, continuity and high efficiency are achieved in the total processing process, and the extraction rate and content of the ganoderan can be greatly raised.
Description
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of ultrasonic wave method for extracting of ganoderan.
Background technology
Ganoderan forms, has the dextran of spirrillum stereo formation (tertiary structure) by three strands of monose chains, its stereo formation and thymus nucleic acid (DNA), Yeast Nucleic Acid (RNA) are similar, it is a kind of macromolecular cpd, its molecular weight is from thousands of to hundreds thousand of, and it is a kind of material extracted from Ganoderma spore powder or glossy ganoderma.Be insoluble to the ethanol of high density, be slightly soluble in ethanol and the cold water of lower concentration, can all dissolve in the hot water.Ganoderan is all present in the cell walls inwall of glossy ganoderma.Except containing except glucose in ganoderan, mostly also contain the monose such as pectinose, wood sugar, semi-lactosi, Fucose, seminose, rhamnosyl, but content is less.
Traditional Extraction method of ganoderan mainly adopts Hot water extraction, acidleach formulation, alkali method to extract.Though acid or alkali method can improve extraction yield, high and very easily destroy the structure of polysaccharide to equipment requirements, also there is problem of environmental pollution.In addition because Ganoderma sporophore is made up of main components such as Mierocrystalline cellulose, hemicellulose and xylogen, close structure, active polysaccharide effective constituent is attached to inside cell walls, and traditional Hot water extraction time is grown, it is high to consume energy, and active component extract yield is low.Chinese patent CN1181271 describes and uses low pressure boiled process, and the pharmacologically active of polysaccharide is higher than conventional poach, but polysaccharide extract rate is on the low side, and extract obtained middle Ganoderma triterpenoids acid content is also on the low side.Chinese patent CN1560072 is by adopting supercritical CO
2extracting ganoderma triterpenic acid extracting solution, raffinate obtains ganoderma lucidum polysaccharide extract through hot water extraction again.But the method required equipment drops into comparatively large, operation easier and production cost is also higher, and ganoderan extraction yield is on the low side.
Summary of the invention
(1) technical problem solved
For the deficiencies in the prior art, the invention provides a kind of ultrasonic wave method for extracting of ganoderan.The present invention not only remains the activity of ganoderan to greatest extent, and all the course of processing achieves continuously and high efficiency.
(2) technical scheme
For realizing above object, the present invention is achieved by the following technical programs:
A ultrasonic wave method for extracting for ganoderan, comprises the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 60-80 eye mesh screen after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 100 ~ 200nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: in the glossy ganoderma particulate that Ethanol Treatment is crossed, add prozyme, 1 ~ 4h is processed at 35 ~ 60 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 15 ~ 20 times amount subsequently, refluxing extraction 1 ~ 2h at 95 ~ 100 DEG C, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 85 ~ 95% of 3 ~ 5 times amount, stir, be placed in ultrasonator sonic oscillation 0.5 ~ 1h again, ultrasonic frequency 35 ~ 40KHz, ultrasonic power is 800 ~ 1000W, and leave standstill 12 ~ 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.08 ~ 1.16 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
Preferably, in the composite enzyme solution of described step (1), prozyme is any two or three mixture in papoid, cellulase and polygalacturonase.
Preferably, described composite enzyme solution consumption is add 3mL in every kairine sesame particulate.
Preferably, the described ultrasonic wave alcohol precipitation of described step (4) is: the supernatant liquor obtained in step (3) adds the ethanol of 85 ~ 90% of 4 ~ 5 times amount, stir, be placed in ultrasonator sonic oscillation 0.5 ~ 1h again, ultrasonic frequency 35KHz, ultrasonic power is 800 ~ 900W, leave standstill 12 ~ 24h, centrifuging obtains filter cake, is respectively washed once successively by gained filter cake, be precipitated after cryodrying with 95% ethanol, dehydrated alcohol, ether.
Preferably, described step (3) is described to be slightly extracted as: in the glossy ganoderma particulate that Ethanol Treatment is crossed, add prozyme, 2 ~ 3h is processed at 45 ~ 50 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 16 ~ 18 times amount subsequently, refluxing extraction 1 ~ 2h at 95 ~ 100 DEG C, repeats 2 times, centrifuging and taking supernatant liquor after united extraction liquid.
(3) beneficial effect
The invention provides a kind of ultrasonic wave method for extracting of ganoderan, compared with prior art, there is following beneficial effect:
(1) polysaccharide being present in glossy ganoderma cell inwall can farthest discharge by the nano-pulverization means that the present invention adopts;
(2) in scheme provided by the invention, first with after Ethanol Treatment, to remove lipid material, make Glycosylase lose activity simultaneously, prevent the degraded of polysaccharide; Adopt complex enzyme degradation outer layer fiber and pectin, be more conducive to the extraction of polysaccharide, the extraction yield of ganoderan and content can be made to obtain larger raising;
(3) the present invention adopts ultrasonic wave method for extracting to extract ganoderan from ultra-fine ganoderma particles, decreases the destruction to ganoderan, significantly improves the extraction yield of effective constituent, reduces extraction time; Adopt ultrasonator to vibrate in alcohol precipitation process, ganoderan in extract can be made to maximize and be dissolved in ethanol, improve the yield of ganoderan;
(4) the inventive method solvent for use safety, avoids in traditional method the pollution adopting the organic solvent such as chloroform, methyl alcohol to environment.
Embodiment
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1:
The ultrasonic wave method for extracting of the present embodiment ganoderan, comprises the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 60 eye mesh screens after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 150nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: the prozyme adding papoid and cellulase composition in the glossy ganoderma particulate that Ethanol Treatment is crossed, add-on is 3mL/g glossy ganoderma particulate, and 3h is processed at 45 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 18 times amount subsequently, refluxing extraction 1h at 95 DEG C, repeats 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 90% of 3 times amount, stir, be placed in ultrasonator sonic oscillation 0.5h again, ultrasonic frequency 35KHz, ultrasonic power is 750W, and leave standstill 18h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.12 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
Embodiment 2:
The ultrasonic wave method for extracting of the present embodiment ganoderan, comprises the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 60 eye mesh screens after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 200nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: the prozyme adding polygalacturonase and cellulase composition in the glossy ganoderma particulate that Ethanol Treatment is crossed, add-on is 3mL/g glossy ganoderma particulate, and 4h is processed at 60 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 20 times amount subsequently, refluxing extraction 2h at 100 DEG C, repeats 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 95% of 5 times amount, stir, be placed in ultrasonator sonic oscillation 1h again, ultrasonic frequency 40KHz, ultrasonic power is 800W, and leave standstill 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.16 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
Embodiment 3:
The ultrasonic wave method for extracting of the present embodiment ganoderan, comprises the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 70 eye mesh screens after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 200nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: the prozyme adding polygalacturonase and cellulase composition in the glossy ganoderma particulate that Ethanol Treatment is crossed, add-on is 3mL/g glossy ganoderma particulate, and 4h is processed at 55 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 20 times amount subsequently, refluxing extraction 2h at 98 DEG C, repeats 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 95% of 5 times amount, stir, be placed in ultrasonator sonic oscillation 1h again, ultrasonic frequency 40KHz, ultrasonic power is 1000W, and leave standstill 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.16 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
Embodiment 4:
The ultrasonic wave method for extracting of the present embodiment ganoderan, comprises the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 80 eye mesh screens after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 200nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: the prozyme adding polygalacturonase and cellulase composition in the glossy ganoderma particulate that Ethanol Treatment is crossed, add-on is 3mL/g glossy ganoderma particulate, and 2h is processed at 45 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 16 times amount subsequently, refluxing extraction 2h at 95 DEG C, repeats 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 85% of 4 times amount, stir, be placed in ultrasonator sonic oscillation 1h again, ultrasonic frequency 40KHz, ultrasonic power is 800W, and leave standstill 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.16 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
Embodiment 5:
The ultrasonic wave method for extracting of the present embodiment ganoderan, comprises the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 80 eye mesh screens after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 150nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: the prozyme adding polygalacturonase and cellulase composition in the glossy ganoderma particulate that Ethanol Treatment is crossed, add-on is 3mL/g glossy ganoderma particulate, and 3h is processed at 50 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 18 times amount subsequently, refluxing extraction 2h at 100 DEG C, repeats 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 90% of 5 times amount, stir, be placed in ultrasonator sonic oscillation 1h again, ultrasonic frequency 40KHz, ultrasonic power is 900W, and leave standstill 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.16 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
It should be noted that, in this article, the such as relational terms of first and second grades and so on is only used for an entity or operation to separate with another entity or operational zone, and not necessarily requires or imply the relation that there is any this reality between these entities or operation or sequentially.And, term " comprises ", " comprising " or its any other variant are intended to contain comprising of nonexcludability, thus make to comprise the process of a series of key element, method, article or equipment and not only comprise those key elements, but also comprise other key elements clearly do not listed, or also comprise by the intrinsic key element of this process, method, article or equipment.When not more restrictions, the key element limited by statement " comprising ... ", and be not precluded within process, method, article or the equipment comprising described key element and also there is other identical element.
Above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit, although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (5)
1. a ultrasonic wave method for extracting for ganoderan, is characterized in that, comprise the following steps:
(1) raw material pre-treatment: by glossy ganoderma through selection, removes mildew and rot, lopsided sporophore, and sporophore crosses 60-80 eye mesh screen after first fragmentation, and subsequently the glossy ganoderma powder after just pulverizing is put into nano grinder and again pulverize, powder particle diameter is 100 ~ 200nm;
(2) degreasing: add 95% alcohol immersion 3 days in glossy ganoderma particulate, elimination ethanol;
(3) slightly extract: in the glossy ganoderma particulate that Ethanol Treatment is crossed, add prozyme, 1 ~ 4h is processed at 35 ~ 60 DEG C, enzymolysis terminates post-heating deactivation prozyme, add the distilled water of 15 ~ 20 times amount subsequently, refluxing extraction 1 ~ 2h at 95 ~ 100 DEG C, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(4) ultrasonic wave alcohol precipitation: the supernatant liquor obtained in step (3) adds the ethanol of 85 ~ 95% of 3 ~ 5 times amount, stir, be placed in ultrasonator sonic oscillation 0.5 ~ 1h again, ultrasonic frequency 35 ~ 40KHz, ultrasonic power is 800 ~ 1000W, and leave standstill 12 ~ 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying;
(5) removing protein and decolouring: dried precipitation is dissolved in distilled water, add tan-liquor under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor, add 0.1% activated carbon decolorizing twice in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(6) ultrafiltration is with concentrated: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, and it is 1.08 ~ 1.16 that filtered solution is concentrated into relative density, makes concentrated solution;
(7) purifying: by concentrated solution respectively with the borax of 0.025mol/L, 0.1mol/L, the saturated sodium hydroxide solution of 0.1mol/L carries out wash-out, then the Yong copper sulfuric acid liquid colorimetric estimation optical density of 0.2% is used, collect the elutriant having polysaccharide, concentrating and desalinating final vacuum is drying to obtain ganoderan.
2. the ultrasonic wave method for extracting of ganoderan according to claim 1, is characterized in that, in the composite enzyme solution of described step (1), prozyme is two or three mixture in papoid, cellulase and polygalacturonase.
3. the ultrasonic wave method for extracting of ganoderan according to claim 1 and 2, is characterized in that, described composite enzyme solution consumption is add 3mL in every kairine sesame particulate.
4. the ultrasonic wave method for extracting of ganoderan according to claim 1, it is characterized in that, the described ultrasonic wave alcohol precipitation of described step (4) is: the supernatant liquor obtained in step (3) adds the ethanol of 85 ~ 90% of 4 ~ 5 times amount, stir, be placed in ultrasonator sonic oscillation 0.5 ~ 1h again, ultrasonic frequency 35KHz, ultrasonic power is 800 ~ 900W, leave standstill 12 ~ 24h, centrifuging obtains filter cake, gained filter cake is respectively washed once with 95% ethanol, dehydrated alcohol, ether successively, is precipitated after cryodrying.
5. the ultrasonic wave method for extracting of ganoderan according to claim 1, it is characterized in that, described step (3) is described to be slightly extracted as: in the glossy ganoderma particulate that Ethanol Treatment is crossed, add prozyme, 2 ~ 3h is processed at 45 ~ 50 DEG C, enzymolysis terminates post-heating deactivation prozyme, adds the distilled water of 16 ~ 18 times amount subsequently, refluxing extraction 1 ~ 2h at 95 ~ 100 DEG C, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid.
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| CN106008732A (en) * | 2016-06-06 | 2016-10-12 | 福建山之灵生物科技股份有限公司 | Extraction method for dynamic cycle extraction of ganoderma lucidum through combination of enzymolysis and ultrasonic waves |
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| CN108250320B (en) * | 2018-03-09 | 2020-04-21 | 杨凌萃健生物工程技术有限公司 | Low-ash ganoderma lucidum polysaccharide extract and preparation method thereof |
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| CN110922447A (en) * | 2019-12-31 | 2020-03-27 | 武夷山元生泰生物科技有限公司 | Method for extracting triterpenic acid and polysaccharide from ganoderma lucidum |
| CN114272274A (en) * | 2021-12-08 | 2022-04-05 | 安徽省双辉生物科技有限公司 | Ganoderma lucidum composition for improving immunity and preparation method thereof |
| CN115558037A (en) * | 2022-10-19 | 2023-01-03 | 陕西科技大学 | Ganoderma lucidum beta-glucan extract and preparation method and detection method thereof |
| CN115558037B (en) * | 2022-10-19 | 2024-02-06 | 陕西科技大学 | Ganoderma lucidum beta-glucan extract and preparation method and detection method thereof |
| CN116694721A (en) * | 2023-08-09 | 2023-09-05 | 长春中医药大学 | Ganoderma lucidum glycopeptide and preparation method and application thereof, and ganoderma lucidum glycopeptide microemulsion and face cream |
| CN116694721B (en) * | 2023-08-09 | 2023-10-20 | 长春中医药大学 | Ganoderma lucidum glycopeptide and preparation method and application thereof, and ganoderma lucidum glycopeptide microemulsion and face cream |
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