CN113024682A - Method for extracting ganoderma lucidum spore powder polysaccharide - Google Patents
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Abstract
The invention discloses a method for extracting ganoderma lucidum spore powder polysaccharide, which comprises the steps of pretreatment; breaking cell wall; ultrasonic extraction; concentrating; and (5) freeze drying and the like. The ganoderma lucidum spore wall breaking method adopts enzymolysis and microwave wall breaking, effectively improves the ganoderma lucidum spore wall breaking rate, has the wall breaking rate as high as 99 percent, and can effectively retain the effective components in the raw materials; the biological activity of the ganoderma lucidum spore powder polysaccharide is effectively kept by the ultrasonic extraction and vacuum freeze drying, the influence of physicochemical factors such as solvent, temperature, pressure and the like is avoided, the ganoderma lucidum spore powder polysaccharide with sweet sucrose, high purity and no organic solvent residue is finally obtained, the extraction rate of the ganoderma lucidum spore powder polysaccharide is higher, and the extraction rate is up to 99%.
Description
Technical Field
The invention belongs to the technical field of deep processing of natural products, and particularly relates to a method for extracting ganoderma lucidum spore powder polysaccharide.
Background
Ganoderma lucidum is a large-scale medicinal fungus. It is sweet in nature and neutral in flavor, and enters heart, lung, liver and kidney meridians. Dried fruiting body of Ganoderma lucidum (Leys. exFr.) karst or Ganoderma sinense Zhao, XuetZhang belonging to Polyporaceae of Basidiomycetes. The ganoderma lucidum is characterized in that extremely fine particles ejected from pileus in the later growth and maturation stage of ganoderma lucidum are ganoderma lucidum spore powder, which is called basidiospore in biology, is the germ cells of ganoderma lucidum, has all genetic active substances of ganoderma lucidum, has the main active ingredient content 75 times higher than that of ganoderma lucidum, has polysaccharide, triterpene, protein, amino acid, nucleoside and the like as main active substances, wherein the polysaccharide and the triterpene are the main active substances and are the key points of research of people in recent years. Modern pharmacological research shows that the ganoderma lucidum spore powder has pharmacological effects of improving immunity, resisting oxidation, resisting tumor, resisting aging, protecting liver, regulating blood sugar and the like.
Ganoderma spore (Ganoderma spore) is the seeds of Ganoderma lucidum which is the tiny ovoid germ cells ejected from Ganoderma lucidum fold during the growth and maturation stage. Each ganoderma spore is only 4-6 μm and is in the form of fine powder, so the ganoderma spore powder (ganodermasporopersder) is a living organism, has a double-wall structure, is surrounded by hard chitin cellulose, is difficult to be fully absorbed by a human body, and is more suitable for direct absorption of intestines and stomachs of the human body after wall breaking. The Ganoderma spore has double-wall structure composed of chitin and cellulose, and has hard texture, and the active ingredients are wrapped and not easy to dissolve out, and can influence absorption of human body. Therefore, most spore powder products are subjected to different wall breaking processes to crush spore walls so as to improve the bioavailability of active ingredients in human bodies. However, the broken spore walls are still mixed with the effective components, and the polysaccharide content and the triterpene content of the final product are not improved. The average polysaccharide content of the wall-broken ganoderma lucidum spore powder sold in the market at present is about 1.54 percent, and the triterpene content is about 4.22 percent. The existence of spore powder wall affects the drinking taste, and some people with digestive function insufficiency are easy to cause dyspepsia after drinking in large quantities.
At present, there are some publications on ganoderma lucidum spore extraction, such as: patent application CN201610954473.4 discloses an extraction process of wall-broken ganoderma lucidum spore powder polysaccharide, which comprises the following steps: 1) pre-treating; 2) ultrasonic cavitation; 3) primary purification; 4) concentrating the extract; 5) freeze drying; 6) crushing and collecting: and (3) crushing the freeze-dried powder in order by a crusher, enabling the particles to pass through a screen with 70-90 meshes, bagging and sealing. The process is carried out at low temperature without chemical solvent contact, and effectively retains the bioactivity of wall-broken Ganoderma spore powder polysaccharide, and is free from influence of physicochemical factors such as solvent and temperature. The ganoderma lucidum spore powder polysaccharide extracted by the process has mellow mouthfeel, has no bitter taste of ganoderma lucidum mycelium polysaccharide and sporophore polysaccharide and has sweet and mellow sucrose feeling, and can be directly added into a formula without adding a taste masking agent in a subsequent finished product formula; the polysaccharide material has good fluidity, compressibility and large bulk density, and is suitable for processing into tablet and capsule.
However, the steps for obtaining the wall-broken ganoderma lucidum spore powder are complicated, and the yield of the wall-broken ganoderma lucidum spore powder is not high. The extraction rate of the wall-broken ganoderma lucidum spore powder polysaccharide is not high.
Disclosure of Invention
The invention provides a method for extracting ganoderma lucidum spore powder polysaccharide to solve the technical problems.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a method for extracting ganoderma lucidum spore powder polysaccharide comprises the following steps:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure;
(5) and (3) freeze drying: and (4) sending the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, and then crushing and sieving to obtain the ganoderma lucidum spore powder polysaccharide.
Further, in the step (1), the drying is to dry the ganoderma lucidum spores to a water content of 35-45%.
Further, in the step (2), the complex enzyme is two or more of cellulase, chitinase and glucosidase.
Further, in the step (2), the temperature of the enzymolysis wall breaking is 37-38.5 ℃, and the time is 120-130 min.
Further, in the step (2), the microwave wall breaking power is 600-700W, the temperature is 85-95 ℃, and the time is 155-175 s.
Further, in the step (3), the power of ultrasonic extraction is 90-100W, the frequency is 30-33 kHz, the temperature is 50-55 ℃, the ultrasonic extraction is carried out in a mode of ultrasonic action for 3min and stopping for 2min, and the total extraction time is 15-20 min.
Further, in the step (4), the vacuum degree of the vacuum pumping is-0.2 to-0.15 MPa.
Further, in the step (4), the low-temperature reduced-pressure concentration is carried out at the temperature of 40-45 ℃ and the pressure of 100-200 Pa, and the concentrated extract is concentrated into an extract with the relative density of 1.20-1.25.
Further, in the step (5), the vacuum freeze drying is to freeze at-5 ℃ to-2 ℃ for 8 hours, freeze at-22 ℃ to-20 ℃ for 6 hours, freeze at-26 ℃ for 22 to 24 hours, heat to 28 to 33 ℃, sublime for 20 to 22 hours, and the vacuum degree in the whole freeze drying process is-0.2 MPa to-0.1 MPa.
Further, in the step (5), the sieving is performed by a sieve with 50-60 meshes.
Due to the adoption of the technical scheme, the invention has the following beneficial effects:
(1) the ganoderma lucidum spore wall breaking method adopts enzymolysis and microwave wall breaking, effectively improves the ganoderma lucidum spore wall breaking rate, has the wall breaking rate as high as 99 percent, and can effectively retain the effective components in the raw materials.
(2) According to the method, the biological activity of the ganoderma lucidum spore powder polysaccharide is effectively kept through the ultrasonic method extraction and the vacuum freeze drying, the influence of physicochemical factors such as solvent, temperature and pressure is avoided, the ganoderma lucidum spore powder polysaccharide with sweet sucrose, high purity and no organic solvent residue is finally obtained, the extraction rate of the ganoderma lucidum spore powder polysaccharide is high, and the extraction rate is up to 99%.
(3) The method does not adopt any chemical substance, especially organic solvent ethanol, and has the advantages of simple operation and low cost because the anhydrous ethanol can destroy effective components in the ethanol and even cause residues.
Detailed Description
The following is a detailed description of the embodiments of the present invention, but the present invention is not limited to these embodiments, and any modifications or substitutions in the basic spirit of the embodiments are included in the scope of the present invention as claimed in the claims.
Example 1
A method for extracting ganoderma lucidum spore powder polysaccharide comprises the following steps:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer; the drying is to dry the ganoderma lucidum spores to the water content of 35 percent;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores; the complex enzyme is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis wall breaking is 37 ℃, and the time is 120 min; the microwave wall breaking power is 600W, the temperature is 85 ℃, and the time is 155 s;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution; the ultrasonic extraction power is 90W, the frequency is 30kHz, the temperature is 50 ℃, the ultrasonic extraction is carried out by adopting the mode of ultrasonic action for 3min and stopping for 2min, and the total extraction time is 15 min;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure; the vacuum degree of the vacuumizing is-0.2 MPa; the low-temperature reduced-pressure concentration is carried out at the temperature of 40 ℃ and the pressure of 100Pa, and the low-temperature reduced-pressure concentration is carried out to obtain an extract with the relative density of 1.20;
(5) and (3) freeze drying: feeding the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, then crushing and sieving to obtain ganoderma lucidum spore powder polysaccharide; the vacuum freeze drying is to freeze at-5 ℃ for 8h, at-22 ℃ for 6h, then at-26 ℃ for 22h, then to heat to 28 ℃, the sublimation time is 20h, and the vacuum degree of the whole freeze drying process is-0.2 MPa; the screening is through a 50 mesh screen.
Example 2
A method for extracting ganoderma lucidum spore powder polysaccharide comprises the following steps:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer; the drying is to dry the ganoderma lucidum spores to the water content of 45 percent;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores; the complex enzyme is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis wall breaking is 38.5 ℃, and the time is 130 min; the microwave wall breaking power is 700W, the temperature is 95 ℃, and the time is 175 s;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution; the ultrasonic extraction power is 100W, the frequency is 33kHz, the temperature is 55 ℃, the ultrasonic extraction is carried out by adopting the mode of ultrasonic action for 3min and stopping for 2min, and the total extraction time is 20 min;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure; the vacuum degree of the vacuumizing is-0.15 MPa; the low-temperature reduced-pressure concentration is carried out at the temperature of 45 ℃ and the pressure of 200Pa, and the low-temperature reduced-pressure concentration is carried out to obtain an extract with the relative density of 1.25;
(5) and (3) freeze drying: feeding the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, then crushing and sieving to obtain ganoderma lucidum spore powder polysaccharide; the vacuum freeze drying is to freeze at-2 ℃ for 8h, at-20 ℃ for 6h, then at-26 ℃ for 24h, then to heat to 33 ℃, the sublimation time is 22h, and the vacuum degree of the whole freeze drying process is-0.1 MPa; the screening is through a 60 mesh screen.
Example 3
A method for extracting ganoderma lucidum spore powder polysaccharide comprises the following steps:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer; the drying is to dry the ganoderma lucidum spores to the water content of 37 percent;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores; the complex enzyme is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis wall breaking is 37.5 ℃, and the time is 122 min; the microwave wall breaking power is 620W, the temperature is 87 ℃, and the time is 160 s;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution; the ultrasonic extraction power is 92W, the frequency is 31kHz, the temperature is 51 ℃, the ultrasonic extraction is carried out by adopting the mode of ultrasonic action for 3min and stopping for 2min, and the total extraction time is 16 min;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure; the vacuum degree of the vacuumizing is-0.16 MPa; the low-temperature reduced-pressure concentration is carried out at the temperature of 41 ℃ and the pressure of 120Pa, and the low-temperature reduced-pressure concentration is carried out to obtain an extract with the relative density of 1.21;
(5) and (3) freeze drying: feeding the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, then crushing and sieving to obtain ganoderma lucidum spore powder polysaccharide; the vacuum freeze drying is to freeze at-4 ℃ for 8h, at-22 ℃ for 6h, then at-26 ℃ for 22h, then to heat to 29 ℃, the sublimation time is 20h, and the vacuum degree of the whole freeze drying process is-0.18 MPa; the screening was through a 53 mesh screen.
Example 4
A method for extracting ganoderma lucidum spore powder polysaccharide comprises the following steps:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer; the drying is to dry the ganoderma lucidum spores to the water content of 42 percent;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores; the complex enzyme is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis wall breaking is 38 ℃, and the time is 128 min; the microwave wall breaking power is 680W, the temperature is 92 ℃, and the time is 170 s;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution; the ultrasonic extraction power is 98W, the frequency is 32kHz, the temperature is 54 ℃, the ultrasonic extraction is carried out by adopting the mode of ultrasonic action for 3min and stopping for 2min, and the total extraction time is 18 min;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure; the vacuum degree of the vacuumizing is-0.18 MPa; the low-temperature reduced-pressure concentration is carried out at the temperature of 44 ℃ and the pressure of 180Pa, and the low-temperature reduced-pressure concentration is carried out to obtain an extract with the relative density of 1.24;
(5) and (3) freeze drying: feeding the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, then crushing and sieving to obtain ganoderma lucidum spore powder polysaccharide; the vacuum freeze drying is to freeze at-3 ℃ for 8h, at-20 ℃ for 6h, then at-26 ℃ for 24h, then to heat to 32 ℃, the sublimation time is 22h, and the vacuum degree of the whole freeze drying process is-0.14 MPa; the screening was through a 58 mesh screen.
Example 5
A method for extracting ganoderma lucidum spore powder polysaccharide comprises the following steps:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer; the drying is to dry the ganoderma lucidum spores to the water content of 40 percent;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores; the complex enzyme is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis wall breaking is 37.7 ℃, and the time is 125 min; the microwave wall breaking power is 650W, the temperature is 90 ℃, and the time is 165 s;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution; the ultrasonic extraction power is 95W, the frequency is 32kHz, the temperature is 53 ℃, the ultrasonic extraction is carried out by adopting the mode of ultrasonic action for 3min and stopping for 2min, and the total extraction time is 17 min;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure; the vacuum degree of the vacuumizing is-0.17 MPa; the low-temperature reduced-pressure concentration is carried out at the temperature of 43 ℃ and the pressure of 150Pa, and the low-temperature reduced-pressure concentration is carried out to obtain an extract with the relative density of 1.23;
(5) and (3) freeze drying: feeding the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, then crushing and sieving to obtain ganoderma lucidum spore powder polysaccharide; the vacuum freeze drying is to freeze at-3 ℃ for 8h, at-21 ℃ for 6h, then at-26 ℃ for 23h, then to heat to 30 ℃, the sublimation time is 21h, and the vacuum degree of the whole freeze drying process is-0.15 MPa; the screening is through a 55 mesh screen.
Comparative example 1
The extraction of ganoderma lucidum spore powder polysaccharide was performed according to the example of patent application CN201610954473.4 (an extraction process of wall-broken ganoderma lucidum spore powder polysaccharide).
To further illustrate that the present invention can achieve the technical effects, the following experiments were performed:
and (3) extracting the ganoderma lucidum spore powder polysaccharide according to the methods of the examples 1-5 and the comparative example 1, and recording the wall breaking rate of the ganoderma lucidum spores and the extraction rate of the ganoderma lucidum spore powder polysaccharide.
TABLE 1
| Group of | Wall breaking rate | Extraction rate |
| Example 1 | 98.2％ | 99.0％ |
| Example 2 | 98.5％ | 98.6％ |
| Example 3 | 99.0％ | 98.8％ |
| Example 4 | 98.7％ | 99.0％ |
| Example 5 | 99.0％ | 99.0％ |
| Comparative example 1 | 89.9％ | 91.3％ |
Therefore, the extraction process effectively improves the wall-breaking rate of the ganoderma lucidum spores, and the wall-breaking rate is as high as 99%; and the extraction rate of the ganoderma lucidum spore powder polysaccharide is improved and reaches up to 99 percent.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (10)
1. The method for extracting the ganoderma lucidum spore powder polysaccharide is characterized by comprising the following steps of:
(1) pretreatment: selecting fresh Ganoderma spore wet thallus without insect and rot, removing impurities, cleaning, and oven drying in a dryer;
(2) wall breaking treatment: putting the ganoderma lucidum spores pretreated in the step (1) into a complex enzyme solution for enzymolysis wall breaking, uniformly stirring, and then performing microwave wall breaking treatment to obtain wall-broken ganoderma lucidum spores;
(3) ultrasonic extraction: adding purified water into the wall-broken ganoderma lucidum spores subjected to the wall-breaking treatment in the step (2), and then sending the mixture into an ultrasonic reaction kettle for ultrasonic extraction to obtain an extracting solution;
(4) concentration: vacuumizing the extracting solution obtained in the step (3), standing, taking supernate, and concentrating the supernate into ganoderma lucidum spore extract at low temperature under reduced pressure;
(5) and (3) freeze drying: and (4) sending the ganoderma lucidum spore extract obtained in the step (4) into a freeze drying chamber for vacuum freeze drying, and then crushing and sieving to obtain the ganoderma lucidum spore powder polysaccharide.
2. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (1), the drying is to dry the ganoderma lucidum spores until the water content is 35-45%.
3. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (2), the complex enzyme is two or more of cellulase, chitinase and glucosidase.
4. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (2), the temperature of the enzymolysis wall breaking is 37-38.5 ℃, and the time is 120-130 min.
5. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (2), the microwave wall breaking power is 600-700W, the temperature is 85-95 ℃, and the time is 155-175 s.
6. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (3), the power of ultrasonic extraction is 90-100W, the frequency is 30-33 kHz, the temperature is 50-55 ℃, the ultrasonic extraction is stopped for 2min after 3min, and the total extraction time is 15-20 min.
7. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (4), the vacuum degree of the vacuum pumping is-0.2 to-0.15 MPa.
8. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (4), the low-temperature reduced pressure concentration is carried out at the temperature of 40-45 ℃ and the pressure of 100-200 Pa, and the low-temperature reduced pressure concentration is carried out to obtain an extract with the relative density of 1.20-1.25.
9. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (5), the vacuum freeze drying is to freeze for 8 hours at-5 to-2 ℃, freeze for 6 hours at-22 to-20 ℃, then freeze for 22 to 24 hours at-26 ℃, heat to 28 to 33 ℃, sublime for 20 to 22 hours, and the vacuum degree of the whole freeze drying process is-0.2 to-0.1 MPa.
10. The method for extracting ganoderma lucidum spore powder polysaccharide as claimed in claim 1, wherein the method comprises the following steps: in the step (5), the sieving is performed by a sieve with 50-60 meshes.
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| CN114533770A (en) * | 2022-02-22 | 2022-05-27 | 西藏天虹科技股份有限责任公司 | Extraction process and device of ganoderma lucidum extract |
| CN115725005A (en) * | 2022-12-19 | 2023-03-03 | 西藏天虹科技股份有限责任公司 | Preparation method of high-purity ganoderma lucidum polysaccharide |
| CN115944078A (en) * | 2022-12-20 | 2023-04-11 | 南京中科药业有限公司 | Method for improving fluidity of wall-broken ganoderma lucidum spore powder |
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| CN114533770B (en) * | 2022-02-22 | 2024-02-06 | 西藏天虹科技股份有限责任公司 | Extraction process and device of ganoderma lucidum extract |
| CN115725005A (en) * | 2022-12-19 | 2023-03-03 | 西藏天虹科技股份有限责任公司 | Preparation method of high-purity ganoderma lucidum polysaccharide |
| CN115944078A (en) * | 2022-12-20 | 2023-04-11 | 南京中科药业有限公司 | Method for improving fluidity of wall-broken ganoderma lucidum spore powder |
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