CN105902578A - Extraction method of triterpenoid in Ganoderma lucidum spore - Google Patents
Extraction method of triterpenoid in Ganoderma lucidum spore Download PDFInfo
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- CN105902578A CN105902578A CN201610268953.5A CN201610268953A CN105902578A CN 105902578 A CN105902578 A CN 105902578A CN 201610268953 A CN201610268953 A CN 201610268953A CN 105902578 A CN105902578 A CN 105902578A
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- ganoderma spore
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- 238000000605 extraction Methods 0.000 title claims abstract description 12
- 240000008397 Ganoderma lucidum Species 0.000 title abstract description 10
- 235000001637 Ganoderma lucidum Nutrition 0.000 title abstract description 10
- XBZYWSMVVKYHQN-MYPRUECHSA-N (4as,6as,6br,8ar,9r,10s,12ar,12br,14bs)-10-hydroxy-2,2,6a,6b,9,12a-hexamethyl-9-[(sulfooxy)methyl]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-4a-carboxylic acid Chemical compound C1C[C@H](O)[C@@](C)(COS(O)(=O)=O)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C XBZYWSMVVKYHQN-MYPRUECHSA-N 0.000 title abstract 3
- 239000000284 extract Substances 0.000 claims abstract description 17
- 241000222336 Ganoderma Species 0.000 claims description 84
- 239000000843 powder Substances 0.000 claims description 65
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 42
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 42
- -1 triterpenoid compound Chemical class 0.000 claims description 28
- 238000001914 filtration Methods 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 22
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 18
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 14
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 14
- 230000001794 chitinolytic effect Effects 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 14
- 238000010828 elution Methods 0.000 claims description 14
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 9
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 9
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 9
- 229960005070 ascorbic acid Drugs 0.000 claims description 9
- 235000010323 ascorbic acid Nutrition 0.000 claims description 9
- 239000011668 ascorbic acid Substances 0.000 claims description 9
- 239000003463 adsorbent Substances 0.000 claims description 8
- 239000011347 resin Substances 0.000 claims description 8
- 229920005989 resin Polymers 0.000 claims description 8
- 229960003237 betaine Drugs 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 7
- 238000007654 immersion Methods 0.000 claims description 7
- 239000003208 petroleum Substances 0.000 claims description 7
- 238000010992 reflux Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 238000003828 vacuum filtration Methods 0.000 claims description 7
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 5
- 241000233866 Fungi Species 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229920002101 Chitin Polymers 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000002622 anti-tumorigenesis Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 210000005132 reproductive cell Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of fungus treatment, in particular to an extraction method of triterpenoid in Ganoderma lucidum spore. The extraction method is performed by means of wall breaking first and extracting second, and extract obtained from Ganoderma lucidum spore is mainly triterpenoid. The extraction method is low in technical difficulty, and active ingredients of Ganoderma lucidum spore easier for human body to absorb can be obtained, so that utilization rate of Ganoderma lucidum spore is increased.
Description
Technical field
The present invention relates to mushroom processing technology field, be specifically related to the extracting method of triterpenoid compound in a kind of Ganoderma spore powder.
Background technology
Ganoderma spore is the sexual reproductive cell of Ganoderma, containing whole genetic activities materials of Ganoderma, has the effect such as antitumor, blood sugar lowering and immunomodulating, in pharmacology with there is application clinically, is also a kind of good natural health-care products raw material.Ganoderma spore contains the antitumorigenic substances such as abundant polysaccharide, triterpenoid compound, polypeptide, nucleic acid, organic germanium, and ganoderma lucidum triterpene compounds is antineoplastic main functional component.Ganoderma lucidum triterpene compounds is present in Ganoderma spore oil, has physiology and pharmacologically active widely, and its exploitation has wide market prospect with application.
Ganoderma spore powder be Ganoderma in the growth and maturity phase, the most small avette sexual cell shot out of being hit by a bullet from Ganoderma lamella, i.e. the seed of Ganoderma, it has condensed the elite of Ganoderma, has had whole hereditary materials and the health-care effect of Ganoderma.But Ganoderma spore wall has two-layer, internal containing 1-2 oil droplet, outer wall is made up of one layer of hard chitin mass shell, insoluble fibre and cellulose, there is the strongest resistance to acids and bases, prevent solvent that the extraction of Ganoderma spore oil is processed, only opening this double wall shell, its effective ingredient just can be immediately used by the body absorption.Scientific experiments confirms, taking the spore of non-breaking cellular wall, the effective ingredient of only 10%～20% can be absorbed by the body, and after breaking cellular wall, effective ingredient absorbance is more than 90%.
Ganoderma lucidum triterpene compounds is present in Ganoderma spore oil, is the main functional component of Ganoderma spore, breaking trachytectum of glossy ganoderma be Ganoderma spore Related product must be through operation.Extracting method many employings acid-base method of Ganoderma spore oil at present, enzyme process, defect is that wall breaking rate of ganoderma lucidum spores is low, and Ganoderma spore oil yield is low, the most also can destroy the effective ingredient of Ganoderma spore oil；Additionally, also microwave method, supercritical ultrasonics technology, liquid nitrogen quenching method, although these methods can improve Ganoderma spore oil yield, but working (machining) efficiency is low, and cost is high, is only applicable to laboratory operation, it is difficult to realize industrialized production.Although having multiple technologies at present can complete the broken wall treatment to Ganoderma spore powder, but the triterpenes Ganodenic acid unstable chemcial property contained by Ganoderma spore powder, while completing breaking cellular wall, how to ensure that effective component of glossy ganoderma is not destroyed during breaking cellular wall to greatest extent, and complete after breaking cellular wall again can stable existence, be the most still a technical barrier not having to solve.It is therefore desirable to extract triterpenoid compound to improve its utilization rate.
Summary of the invention
The invention aims to solve the problems referred to above, it is provided that the extracting method of triterpenoid compound in a kind of Ganoderma spore powder.
Here final products are the extract of Ganoderma spore powder, and are mainly composed of the mixture of triterpenoid compound.
In order to reach foregoing invention purpose, the present invention by the following technical solutions:
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder, comprises the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.05-0.15%, ultrasonic immersion 1-3h；Then add water and chitinolytic enzyme after filtering, decompose 1-2h, be dried at 35-50 DEG C after filtration；Drying time is at 5-10h；Ultrasonic power is 0.8-1.5w/cm2, frequency is 35-40KHz；
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-10 DEG C to-5 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2-4 time, vacuum filtration；Petroleum ether microwave counter current is used to extract 1-3 time filtering residue the most again；
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；Add trehalose and can promote the adsorbent resin absorption to effective ingredient；Add trehalose and improve the rate of adsorption.
(5) then use concentration expressed in percentage by volume for 8-10 column volume of 60-70% ethanol elution, collecting 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
As preferably, in step (1), the ultrasonic Ganoderma spore powder soaked after filtering is added to the water, and in Ganoderma spore powder, the addition of chitinolytic enzyme is 3000-4000u/g, adds ascorbic acid 3-5g in the most every 1kg Ganoderma spore powder.
Ascorbic acid controls the activity of enzyme, it is to avoid enzyme, by the chitin excessive decomposition of spore powder outer wall, causes loss.
As preferably, the ethanol water kind in step (5) is additionally added the glycine betaine that mass percent is 0.05-0.1%.
Add glycine betaine and can accelerate the extract i.e. abjection of triterpenoid compound.
As preferably, in step (1), the consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml.
As preferably, the extraction time that in step (3), each microwave counter current extracts is 10-15min.
The present invention compared with prior art, provides the benefit that:
1 method and technology difficulty is relatively low；2 can obtain the effective component of ganoderma lucidum spore powder being easier to be absorbed by the body, thus improve the utilization rate of Ganoderma spore powder.
Detailed description of the invention
Below by specific embodiment, technical scheme is further described explanation.
If without specified otherwise, the raw material employed in embodiments of the invention is raw material commonly used in the art, and the method employed in embodiment is the conventional method of this area.
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder, comprises the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.05-0.15%, ultrasonic immersion 1-3h；Then add water and chitinolytic enzyme after filtering, decompose 1-2h, be dried at 35-50 DEG C after filtration；Drying time is at 5-10h；Ultrasonic power is 0.8-1.5w/cm2, frequency is 35-40KHz；The consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml, and in Ganoderma spore powder, the addition of chitinolytic enzyme is 3000-4000u/g, adds ascorbic acid 3-5g in the most every 1kg Ganoderma spore powder,
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-10 DEG C to-5 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2-4 time, vacuum filtration；Petroleum ether microwave counter current is used to extract 1-3 time filtering residue the most again；The extraction time that microwave counter current extracts every time is 10-15min；
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；
(5) then using concentration expressed in percentage by volume for using 8-10 column volume of 60-70% ethanol elution, ethanol water kind is additionally added the glycine betaine that mass percent is 0.05-0.1%, collects 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
Embodiment
1
:
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder, comprises the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.05%, ultrasonic immersion 3h；Then adding water and chitinolytic enzyme after filtering, in Ganoderma spore powder, the addition of chitinolytic enzyme is 3000u/g, adds ascorbic acid 5g, decomposes 1h, be dried after filtration at 50 DEG C in the most every 1kg Ganoderma spore powder；Drying time is at 5h；Ultrasonic power is 1.5w/cm2, frequency is 35KHz；The consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml；
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-5 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2 times, vacuum filtration；Petroleum ether microwave counter current is used to extract 3 times filtering residue the most again；The extraction time that microwave counter current extracts every time is 10-15min
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；
(5) then use concentration expressed in percentage by volume for 8-10 column volume of 60-70% ethanol elution, collecting 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
Embodiment
2
:
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder, comprises the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.15%, ultrasonic immersion 1h；Then adding water and chitinolytic enzyme after filtering, in Ganoderma spore powder, the addition of chitinolytic enzyme is 4000u/g, adds ascorbic acid 3g, decomposes 2h, be dried after filtration at 35 DEG C in the most every 1kg Ganoderma spore powder；Drying time is at 10h；Ultrasonic power is 0.8w/cm2, frequency is 40KHz；The consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml；
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-10 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2-4 time, vacuum filtration；Petroleum ether microwave counter current is used to extract 1-3 time filtering residue the most again；The extraction time that microwave counter current extracts every time is 10-15min
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；
(5) then use concentration expressed in percentage by volume for 8-10 column volume of 60-70% ethanol elution, collecting 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
Embodiment
3
:
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder, comprises the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.05%, ultrasonic immersion 3h；Then adding water and chitinolytic enzyme after filtering, decompose 1h, in Ganoderma spore powder, the addition of chitinolytic enzyme is 4000u/g, adds ascorbic acid 3g in the most every 1kg Ganoderma spore powder；It is dried at 50 DEG C after filtration；Drying time is at 5h；Ultrasonic power is 1.5w/cm2, frequency is 35KHz；The consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml；
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-5 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2-4 time, vacuum filtration；Petroleum ether microwave counter current is used to extract 1-3 time filtering residue the most again；The extraction time that microwave counter current extracts every time is 10-15min；
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；
(5) then using concentration expressed in percentage by volume for using 8-10 column volume of 60-70% ethanol elution, ethanol water kind is additionally added the glycine betaine that mass percent is 0.05%, collects 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
Embodiment
4
:
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder, comprises the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.15%, ultrasonic immersion 1h；Then adding water and chitinolytic enzyme after filtering, decompose 2h, in Ganoderma spore powder, the addition of chitinolytic enzyme is 3000u/g, adds ascorbic acid 5g in the most every 1kg Ganoderma spore powder；It is dried at 35 DEG C after filtration；Drying time is at 10h；Ultrasonic power is 0.8w/cm2, frequency is 40KHz；The consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml；
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-10 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2-4 time, vacuum filtration；Petroleum ether microwave counter current is used to extract 1-3 time filtering residue the most again；The extraction time that microwave counter current extracts every time is 10-15min；
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；
(5) then using concentration expressed in percentage by volume for using 8-10 column volume of 60-70% ethanol elution, ethanol water kind is additionally added the glycine betaine that mass percent is 0.1%, collects 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
Step (1) adds ascorbic acid than the yield rate being not added with improving 3-5%；Fast speed 3-7% can be added after step (5) adds glycine betaine.
Claims (5)
1. the extracting method of triterpenoid compound in a Ganoderma spore powder, it is characterised in that comprise the following steps:
(1) first Ganoderma spore powder is put in the sodium hydroxide solution that mass concentration is 0.05-0.15%, ultrasonic immersion 1-3h；Then add water and chitinolytic enzyme after filtering, decompose 1-2h, be dried at 35-50 DEG C after filtration；Drying time is at 5-10h；Ultrasonic power is 0.8-1.5w/cm2, frequency is 35-40KHz；
(2) dried Ganoderma spore powder employing supersonic jet mill is pulverized at-10 DEG C to-5 DEG C；
(3) addition Ganoderma spore powder is joined in microwave reflux extracting device, add in water and mixed liquor that butanol proportioning is 3:1 and extract 2-4 time, vacuum filtration；Petroleum ether microwave counter current is used to extract 1-3 time filtering residue the most again；
(4) in two kinds of filtrate, add trehalose, after stirring, all use macroporous adsorbent resin to adsorb；
(5) then use concentration expressed in percentage by volume for 8-10 column volume of 60-70% ethanol elution, collecting 60-70% ethanol elution, concentrating under reduced pressure, reclaim ethanol, drying under reduced pressure, obtain triterpenoid compound.
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder the most according to claim 1, it is characterized in that, in step (1), the ultrasonic Ganoderma spore powder soaked after filtering is added to the water, in Ganoderma spore powder, the addition of chitinolytic enzyme is 3000-4000u/g, adds ascorbic acid 3-5g in the most every 1kg Ganoderma spore powder.
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder the most according to claim 1, it is characterised in that the ethanol water kind in step (5) is additionally added the glycine betaine that mass percent is 0.05-0.1%.
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder the most according to claim 1, it is characterised in that in step (1), the consumption of sodium hydroxide solution is every gram of Ganoderma spore powder 10ml.
The extracting method of triterpenoid compound in a kind of Ganoderma spore powder the most according to claim 1, it is characterised in that the extraction time that in step (3), each microwave counter current extracts is 10-15min.
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| CN113024682A (en) * | 2021-03-16 | 2021-06-25 | 贵州高山生物科技有限公司 | Method for extracting ganoderma lucidum spore powder polysaccharide |
| CN114618689A (en) * | 2022-05-16 | 2022-06-14 | 广东华夏友美生物科技有限公司 | Ganoderma triterpene extraction and separation equipment and extraction and separation process thereof |
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