CN107242578B - Method for extracting soybean fiber and bean dreg protein by homogenizing auxiliary alkaline method - Google Patents

Method for extracting soybean fiber and bean dreg protein by homogenizing auxiliary alkaline method Download PDF

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CN107242578B
CN107242578B CN201710538836.0A CN201710538836A CN107242578B CN 107242578 B CN107242578 B CN 107242578B CN 201710538836 A CN201710538836 A CN 201710538836A CN 107242578 B CN107242578 B CN 107242578B
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赵强忠
赵谋明
蔡勇建
苏国万
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Guangdong wenbang Biotechnology Co.,Ltd.
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South China University of Technology SCUT
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/001Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
    • A23J1/005Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from vegetable waste materials

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  • Food Science & Technology (AREA)
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  • Health & Medical Sciences (AREA)
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  • Mycology (AREA)
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  • Beans For Foods Or Fodder (AREA)
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  • Peptides Or Proteins (AREA)

Abstract

The invention belongs to the technical field of deep processing of soybean byproducts, and discloses a method for extracting soybean fiber and bean dreg protein by combining a homogeneous auxiliary alkaline method. The method comprises the following steps: sieving bean dreg powder, dispersing the sieved bean dreg powder into deionized water, homogenizing for 1-3 times at 300-600 bar, adjusting the pH value to 9.0-13.0, and then stirring and ultrafiltering to obtain filter residues and filtrate; re-dissolving the filter residue in deionized water, adjusting pH to neutral, and drying to obtain soybean fiber; adjusting the pH value of the obtained filtrate to 3.0-5.0, standing at room temperature, centrifuging, re-dissolving the obtained precipitate in deionized water, adjusting the pH value to be neutral, and drying to obtain the bean dreg protein. The method can simultaneously extract two main substances in the bean dregs, breaks through the limitation that only one substance in the bean dregs can be extracted in the past, and greatly improves the additional value of the bean dregs by-product; the extracted soybean fiber has the advantages of strong water holding/oil holding capacity, good expansibility, strong water holding/oil holding capacity of the extracted bean dreg protein and the like.

Description

Method for extracting soybean fiber and bean dreg protein by homogenizing auxiliary alkaline method
Technical Field
The invention belongs to the technical field of deep processing of soybean byproducts, and particularly relates to a method for extracting soybean fiber and bean dreg protein by combining a homogeneous auxiliary alkaline method.
Background
China is a large country for producing and processing soybeans, 15-20% of bean dregs are produced in the production and processing process of the soybeans, and currently, about 280 ten thousand tons of bean dregs are produced in China every year. The bean dregs contain more than 70% of cellulose and about 15% of protein, and soybean fiber and bean dregs protein can be further extracted. The soybean fiber has strong physiological activity, the bean dreg protein amino acid composition is reasonable, and the soybean fiber is similar to the soybean protein isolate composition; however, most of soybean fibers are water-insoluble fibers, and the okara protein is tightly combined with other components in the okara, so that the extraction difficulty is very high, the development and application degree of okara resources in China is very low, and the okara is mostly used as animal feed or waste treatment. Therefore, how to reasonably and efficiently develop and apply the soybean fiber and the bean dreg protein plays a key role in improving the fine and further processing of the bean dreg by-product.
Although our country has rich bean dregs resource, the bean dregs have huge development potential, but the development and application of the bean dregs in our country have breakthrough progress at present. The existing development research on the bean dregs mainly focuses on: (1) treating bean dregs by high static pressure, high pressure homogenization, ultrasound and other modes to extract soluble dietary fiber; or optimizing the extraction process of the bean dreg protein by adopting chemical solvent, ultrasonic wave and other modes. These studies focus on the extraction of a single substance from the okara, but only partially increase the extraction rate or purity of such a single substance, and do not significantly improve the functional properties of these extracts; (2) the soybean peptide is prepared by taking the bean dregs as a raw material or fermenting and producing the soybean peptide by taking the bean dregs as a base material by combining an enzymolysis technology or a fermentation technology, and the research is based on the rich nutritive value of the bean dregs and emphasizes the development and application of the whole bean dregs, but the yield or the fermentation effect of the soybean peptide is limited, and the large-scale production is difficult to carry out. In summary, the main reasons for restricting the development and research of the bean dregs are: (1) the extraction difficulty is high, and the extract is single. Due to the limitations of poor solubility of cellulose and high protein binding degree of the soybean dregs, most researches focus on improving the extraction process of soybean fiber or soybean dregs protein, and concentrate on extracting a certain substance in the soybean dregs, so that other substances are easy to discard; (2) the functional characteristics are not good enough. The extraction process affects not only the extraction yield and purity of the material, but also the structural and functional properties of the material. The existing research focuses on optimizing the extraction rate or purity of the soybean fiber or the soybean dreg protein in the soybean dregs, and the change of the functional characteristics of the soybean fiber or the soybean dreg protein in the extraction process is rarely concerned, so that the research for improving the functional characteristics of the soybean fiber or the soybean dreg protein by using the extraction processes is less beneficial.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the invention mainly aims to provide a method for extracting soybean fiber and bean dreg protein by a homogenization auxiliary alkaline method.
Another object of the present invention is to provide a soybean fiber and okara protein obtained by the above method.
The purpose of the invention is realized by the following technical scheme:
a method for extracting soybean fiber and bean dreg protein by a homogenization auxiliary alkaline method in a combined manner comprises the following steps:
(1) sieving the bean dreg powder, dispersing the sieved bean dreg powder in deionized water, and homogenizing for 1-3 times at 300-600 bar; after homogenizing, adjusting the pH value to 9.0-13.0, and then stirring at 300-900 rpm at 25-60 ℃ for 60-120 min; stirring and ultrafiltering to obtain filter residue and filtrate;
(2) re-dissolving the filter residue obtained in the step (1) in deionized water, adjusting the pH value to be neutral, and drying to obtain soybean fiber;
(3) and (2) adjusting the pH value of the filtrate obtained in the step (1) to 3.0-5.0, standing at room temperature, centrifuging, re-dissolving the obtained precipitate in deionized water, adjusting the pH value to be neutral, and drying to obtain the bean dreg protein.
Preferably, the sieving in the step (1) is to sieve the bean dregs with a sieve of 60-120 meshes, and the material-liquid ratio of the sieved bean dregs powder dispersed in the deionized water is 1 (10-40).
Preferably, the ultrafiltration in the step (1) is filtration under the conditions that the pressure is 0.01-0.1 MPa and the filtration pore diameter is 200-400 meshes.
Preferably, the ratio of the filter residue re-dissolved in the deionized water in the step (2) is 1 (4-10).
Preferably, the standing time at room temperature in the step (3) is 30-60 min; the rotating speed of the centrifugation is 4000-8000 rpm, and the time is 10-30 min.
Preferably, the ratio of the precipitate obtained in the step (3) to the feed solution re-dissolved in the deionized water is 1 (4-10).
Preferably, the drying manner in steps (2) and (3) is freeze drying or hot air drying; the freeze drying pressure is 0.01-1 mbar, and the freeze drying time is 12-24 hours; the hot air drying temperature is 40-60 ℃, and the hot air drying time is 4-8 h.
A soybean fiber and bean dregs protein are prepared by the above method.
The principle of the invention is as follows: the bean dregs are pretreated by high-pressure homogenization, so that the tight combination degree of the components of the bean dregs can be obviously weakened, the dispersity of the bean dregs in a solution system is improved, alkali liquor can enter the bean dregs to dissolve out bean dregs protein, and meanwhile, high-shear impact force generated by homogenization is beneficial to activating soybean fibers and improving the water solubility of the bean dregs protein, which is also a key technology for solving the technical problems and patent application protection.
The preparation method and the obtained product have the following advantages and beneficial effects:
(1) the method can simultaneously extract two main substances (soybean fiber and bean dreg protein) in the bean dregs, breaks through the limitation that only one substance in the bean dregs can be extracted in the past, and greatly improves the additional value of the bean dregs by-product;
(2) the method has the advantages of short action time of the homogenization pretreatment and high extraction efficiency; the utilization rate of the bean dreg by-products can be improved to the maximum extent, and the functional characteristics of the bean dreg by-products can be improved, so that the method has important significance for the fine and deep processing of the bean dreg by-products;
(3) the method has the characteristics of simple and easy operation, high extraction efficiency and the like, the extracted soybean fiber has the advantages of strong water holding/oil holding capacity, good expansibility, strong water holding/oil holding capacity of the extracted bean dreg protein and the like.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
Dispersing 100g of bean dregs powder which is sieved by a 120-mesh sieve in deionized water according to the feed-liquid ratio of 1:20g/ml, and homogenizing for 1 time at 600 bar; after homogenization, the pH was adjusted to 13.0, followed by stirring with a stirring paddle at 30 ℃ and 900rpm for 60min (the pH of the okara dispersion was constantly adjusted to 13.0 during stirring); after stirring, adopting a Buchner funnel and a vacuum water circulating pump to carry out ultrafiltration, wherein the ultrafiltration pressure is 0.04 MPa; extracting soybean fiber and bean dreg protein from the filter residue and filtrate obtained by ultrafiltration respectively: re-dissolving the filter residue in deionized water at a ratio of 1:10g/ml, adjusting pH to 7.0, freezing and storing overnight at-24 deg.C, and freeze-drying at 0.01mbar for 12 hr to obtain 75g soybean fiber; adjusting the pH value of the filtrate to 3.8, standing at room temperature for 30min, centrifuging at 8000rpm for 10min, redissolving the centrifuged precipitate in deionized water at a feed-liquid ratio of 1:10g/ml, adjusting the pH value to 7.0, freezing and storing overnight at-24 ℃, and freeze-drying at 0.1mbar for 12h to obtain 14.1g of bean dregs protein.
Example 2
Dispersing 500g of bean dregs powder which is sieved by a 100-mesh sieve in deionized water according to the feed-liquid ratio of 1:30g/ml, and homogenizing for 2 times at 500 bar; after homogenization, the pH was adjusted to 11.0, followed by stirring at 650rpm for 90min at 40 ℃ using a magnetic stirrer (the pH of the okara dispersion was constantly adjusted to 11.0 during stirring); after stirring, adopting a Buchner funnel and a vacuum water circulating pump to carry out ultrafiltration, wherein the ultrafiltration pressure is 0.07 MPa; extracting soybean fiber and bean dreg protein from the filter residue and filtrate obtained by ultrafiltration respectively: re-dissolving the filter residue in deionized water at a ratio of 1:7g/ml, adjusting pH to 7.0, freezing and storing overnight at-24 deg.C, and freeze-drying at 0.05mbar for 16h to obtain 360g soybean fiber; adjusting the pH value of the filtrate to 4.2, standing at room temperature for 40min, centrifuging at 6000 rpm for 20min, redissolving the centrifuged precipitate in deionized water according to the feed-liquid ratio of 1:7g/ml, adjusting the pH value to 7.0, freezing and storing overnight at-24 ℃, and freeze-drying at 0.05mbar for 16h to obtain 69g of bean dregs protein.
Example 3
Dispersing 10Kg of bean dreg powder which is sieved by a sieve with 80 meshes into deionized water according to the feed-liquid ratio of 1:40g/ml, and homogenizing for 3 times at 400 bar; after homogenization, the pH was adjusted to 9.0, followed by stirring with a stirring paddle at 60 ℃ at 400rpm for 120min (the pH of the okara dispersion was constantly adjusted to 9.0 during stirring); after stirring, adopting a Buchner funnel and a vacuum water circulating pump to carry out ultrafiltration, wherein the ultrafiltration pressure is 0.1 MPa; extracting soybean fiber and bean dreg protein from the filter residue and filtrate obtained by ultrafiltration respectively: re-dissolving the filter residue in deionized water at a feed-liquid ratio of 1:4g/ml, adjusting pH to 7.0, and drying with hot air at 50 deg.C for 6 hr to obtain soybean fiber 7 Kg; adjusting the pH value of the filtrate to 4.6, standing at room temperature for 40min, centrifuging at 4000rpm for 30min, redissolving the centrifuged precipitate in deionized water according to the feed-liquid ratio of 1:4g/ml, adjusting the pH value to 7.0, and drying with hot air at 50 ℃ for 6h to obtain 1.29Kg of the bean dreg protein.
Comparative example
Compared with example 1, the method does not adopt 600bar for homogenizing for 1 time, and other steps and conditions are completely the same, so that 52g of soybean fiber is obtained.
The water holding capacity, oil holding capacity and swelling capacity of the products obtained in examples 1 to 3 and comparative example were measured:
method for measuring water (oil) retention: weighing about 0.2g of sample, placing the sample in a pre-weighed centrifugal tube, gradually adding 4-6 g of deionized water (soybean oil) into the centrifugal tube, slightly stirring by using a glass rod, and dispersing until no obvious particles exist; after standing at room temperature for half an hour and centrifuging at 3000 rpm for 20min, the supernatant (not adsorbed oil) was decanted and the water (oil) retention was characterized by the mass of deionized water (soybean oil) absorbed per gram of sample.
Method for measuring dilatability: weighing about 0.1g of soybean fibers, placing the soybean fibers in a 15mL test tube with a plug, and reading the volume of the soybean fibers; slowly adding 10mL of distilled water, standing at room temperature for 24h, and reading the volume of the soybean fiber. The expandability is characterized in terms of the increased volume per g of soybean fibre. Table 1 shows the results of the water retention, oil retention and swelling of the extracted soybean fibers. Table 2 shows the results of water and oil retention measurements of the extracted okara protein and the commercially available soy protein isolate.
TABLE 1 measurement results of Water holding Property, oil holding Property and swelling Properties of Soybean fiber
Figure BDA0001341346410000051
TABLE 2 Water and oil retention Properties of okara protein
Figure BDA0001341346410000052
As can be seen from the results in tables 1 and 2, the soybean fiber extracted by the invention has good water holding/oil holding capacity and strong dispersibility. Okara protein also has good water/oil holding capacity. The soybean fiber obtained without homogenization treatment has common water holding/oil holding property and poor dispersibility.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (8)

1. A method for extracting soybean fiber and bean dreg protein by a homogenization auxiliary alkaline method is characterized by comprising the following steps:
(1) sieving the bean dreg powder, dispersing the sieved bean dreg powder in deionized water, and homogenizing for 1-3 times at 300-600 bar; after homogenizing, adjusting the pH value to 9.0-13.0, and then stirring at 300-900 rpm at 25-60 ℃ for 60-120 min; stirring and ultrafiltering to obtain filter residue and filtrate;
(2) re-dissolving the filter residue obtained in the step (1) in deionized water, adjusting the pH value to be neutral, and drying to obtain soybean fiber;
(3) and (2) adjusting the pH value of the filtrate obtained in the step (1) to 3.0-5.0, standing at room temperature, centrifuging, re-dissolving the obtained precipitate in deionized water, adjusting the pH value to be neutral, and drying to obtain the bean dreg protein.
2. The method of claim 1, wherein the soy fiber and okara protein are extracted by a homogeneous alkaline-assisted process, wherein the homogeneous alkaline-assisted process comprises: the sieving in the step (1) is to sieve the bean dregs with a sieve of 60-120 meshes, and the material-liquid ratio of the sieved bean dregs powder dispersed in deionized water is 1 (10-40).
3. The method of claim 1, wherein the soy fiber and okara protein are extracted by a homogeneous alkaline-assisted process, wherein the homogeneous alkaline-assisted process comprises: the ultrafiltration in the step (1) is carried out under the conditions that the pressure is 0.01-0.1 MPa and the filtration pore diameter is 200-400 meshes.
4. The method of claim 1, wherein the soy fiber and okara protein are extracted by a homogeneous alkaline-assisted process, wherein the homogeneous alkaline-assisted process comprises: and (3) re-dissolving the filter residue in deionized water in the step (2) according to the material-liquid ratio of 1 (4-10).
5. The method of claim 1, wherein the soy fiber and okara protein are extracted by a homogeneous alkaline-assisted process, wherein the homogeneous alkaline-assisted process comprises: standing at room temperature in the step (3) for 30-60 min; the rotating speed of the centrifugation is 4000-8000 rpm, and the time is 10-30 min.
6. The method of claim 1, wherein the soy fiber and okara protein are extracted by a homogeneous alkaline-assisted process, wherein the homogeneous alkaline-assisted process comprises: the ratio of the precipitate obtained in the step (3) to the feed liquid re-dissolved in the deionized water is 1 (4-10).
7. The method of claim 1, wherein the soy fiber and okara protein are extracted by a homogeneous alkaline-assisted process, wherein the homogeneous alkaline-assisted process comprises: the drying mode in the steps (2) and (3) is freeze drying or hot air drying; the freeze drying pressure is 0.01-1 mbar, and the freeze drying time is 12-24 hours; the hot air drying temperature is 40-60 ℃, and the hot air drying time is 4-8 h.
8. A soybean fiber and okara protein, which is characterized in that: prepared by the method of any one of claims 1 to 7.
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* Cited by examiner, † Cited by third party
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豆渣碱溶解特性的研究;郑应华等;《食品工业科技》;20081231;第29卷(第3期);第117-119页,尤其是第117页右栏第2.2节 *
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