JP4878771B2 - Epidermal keratinocyte proliferating agent and use thereof - Google Patents

Description

  The present invention relates to a cereal enzyme degradation product having a function unprecedented by treating cereal with a plurality of enzymes under pressure and heating, a method for producing a cereal enzyme degradation product, and a functional article. In the present invention, the functional article is meant to include a wide range of foods and drinks, skin cosmetics, hair cosmetics, and bath additives.

  Conventionally, for example, rice (see Non-Patent Document 1), fruit (see Non-Patent Document 2), and the like are known and already put into practical use for changing physical properties and the like by applying pressure to food. In this case, it is known that when treated under pressure and heating conditions of a pressure of 30 MPa and a temperature of 25 ° C. or more, the microorganisms are killed and have a bactericidal action (see Non-Patent Document 3). It is also known that allergens can be reduced by high-pressure treatment of food (see Non-Patent Document 4). Moreover, pearl seeds are called Yokuinin and are known to have actions such as drainage, anti-inflammatory, tonic, analgesia (see Non-Patent Document 5).

  Moreover, cosmetics formed by blending a partial hydrolyzate obtained by hydrolyzing a vegetable protein such as soybean with a proteolytic enzyme have been proposed (see Patent Document 1). However, this proposed hydrolysis method consists of a plurality of steps, is a very time-consuming treatment method, and is not practical.

In addition, a food material containing a protein or a degradation product having a high angiotensin converting enzyme inhibitory activity obtained by allowing a protease to act on a protein under pressure and low temperature has been proposed (see Patent Document 2).
However, up to now, the method of treating food materials with multiple types of enzymes, for example, when protease and other enzymes are treated together, the protease inactivates the other enzymes. Is currently difficult and has not been put to practical use.

JP 58-10512 A JP 2002-247955 A “Food and Development”, Vol. 39, No. 12, p. 9, 2004 "FFI Journal" Vol. 210, No. 1, p. 37, 2005 "FFI Journal" Vol. 210, No. 1, Page 4, 2005 "FFI Journal" Vol. 210, No. 1, p. 20, 2005 “Makino Wakahan Herb Encyclopedia”, Hokuryukan, p. 589, 2002

This invention is made | formed in view of this present condition, and makes it a subject to solve the said various problems in the past and to achieve the following objectives. That is, the present invention contains a lot of useful amino acids and monosaccharides by reducing the molecular weight by acting multiple kinds of enzymes on the grain under pressure and heating, and is decomposed by the action of the enzymes. It is an object of the present invention to provide a safe and useful grain enzyme degradation product and a functional article containing the grain enzyme degradation product.
Another object of the present invention is to provide a method for efficiently producing a grain enzyme degradation product in a short time by allowing a plurality of enzymes to act on the grain.

  In order to solve the above-mentioned problems, the present inventors have made extensive studies and as a result, obtained the following knowledge. That is, under pressure and heating, at least two kinds of enzymes having different substrate specificities and reaction rates are reacted with each other to reduce the molecular weight, thereby obtaining a highly functional grain enzyme degradation product. I found out.

The present invention is based on the above findings of the present inventors, and means for solving the above problems are as follows. That is,
<1> A cereal enzyme degradation product obtained by reacting cereals with at least two enzymes having different substrate specificities and reaction rates under pressure and heating.
<2> The above <1>, wherein the cereal is at least one selected from rice, barley, wheat, rye, oats, oats, millet, millet, mackerel, corn, sorghum, azuki bean, soybean, broad bean, mungbean and buckwheat. Is a cereal enzyme degradation product.
<3> The cereal enzyme degradation product according to <2>, wherein the cereal is pearl barley, and the zyme degradation product has at least one of a melanin production inhibitory effect, an epidermal keratinocyte proliferation effect, and a moisturizing effect.
<4> The cereal enzyme degradation product according to any one of <1> to <3>, wherein the enzyme is at least one of a saccharide-degrading enzyme and a proteolytic enzyme.
<5> The cereal enzyme degradation product according to any one of <1> to <4>, wherein the enzyme is at least two selected from amylase, glucosidase, protease, and peptidase.
<6> The grain enzyme degradation product according to any one of <1> to <5>, wherein the reaction is performed at a pressure of 40 to 200 MPa and a temperature of 40 to 80 ° C. for 1 to 36 hours.
<7> A method for producing a cereal enzyme degradation product, comprising adding a saccharide-degrading enzyme and a proteolytic enzyme to cereal and reacting at a pressure of 40 to 200 MPa and a temperature of 40 to 80 ° C. for 1 to 36 hours.
<8> The method for producing a cereal enzyme degradation product according to <7>, wherein protease and amylase are added to the pearl barley and reacted at a pressure of 50 to 150 MPa and a temperature of 50 to 70 ° C. for 3 to 24 hours.
<9> A functional article comprising the grain enzyme degradation product according to any one of <1> to <6>.
<10> The functional article according to <9>, wherein the functional article is any one selected from foods and drinks, skin cosmetics, hair cosmetics, and bath agents.

  According to the present invention, various problems in the prior art can be solved, and a large number of useful amino acids and monosaccharides can be obtained by reducing the molecular weight by causing a plurality of enzymes to act on the grain under pressure and heating, Since it is decomposed | disassembled by the effect | action of an enzyme, a grain enzyme decomposition product safe as a functional material, the manufacturing method of this grain enzyme decomposition product, and a functional article can be provided.

(Grain enzyme degradation product and production method thereof)
The cereal enzyme degradation product of the present invention is obtained by reacting cereals with at least two enzymes having different substrate specificities and reaction rates under pressure and heating.
In the method for producing a cereal enzyme degradation product of the present invention, a saccharide-degrading enzyme and a proteolytic enzyme are added to a cereal, and the reaction is carried out at a pressure of 40 to 200 MPa and a temperature of 40 to 80 ° C. for 1 to 36 hours.
Hereinafter, the details of the method for producing the grain enzyme degradation product of the present invention will be clarified through the description of the grain enzyme degradation product of the present invention.

-Grain-
The cereal is not particularly limited and may be appropriately selected depending on the intended purpose. , Mungbean, buckwheat, etc. These may be used individually by 1 type and may use 2 or more types together.
Among these, pearl barley, wheat, rice, and soybean are preferable, and pearl barley is particularly preferable because the enzyme degradation product has excellent functionality and utility.
There is no restriction | limiting in particular as a use site | part of the said grain, According to the objective, it can select suitably, For example, a seed, a leaf, a root, a nuka etc. are mentioned, A seed is especially preferable. The grain material is preferably dried and pulverized immediately after collection. Drying may be performed in the sun or using a commonly used dryer.

  The pearl barley is a plant belonging to the gramineae family, the scientific name is Coix lacryma-jobi, and its seed is called Yokuinin. Examples of the use area of the pearl barley include seeds, leaves, and roots, and seeds are particularly preferable.

-Enzyme-
The enzyme is not particularly limited as long as it has at least two kinds of substrates having different substrate specificities and reaction rates, and can be appropriately selected according to the purpose. However, at least one of a carbohydrase and a proteolytic enzyme can be used. Is preferred.
Examples of the saccharide-degrading enzyme include amylase, glucosidase (maltase), lysozyme, β-galactosidase, and the like.
The proteolytic enzyme is a generic term for enzymes that hydrolyze proteins, and includes known proteases such as papain, pepsin, trypsin, chymotrypsin, and proteases produced by various microorganisms.
As the enzyme, for example, at least two selected from amylase, glucosidase, protease, and peptidase are preferable, and among these, a combination of amylase and protease is particularly preferable.
The enzyme is not particularly limited as long as it has enzyme activity, and it may be a crude enzyme as well as a purified enzyme.

-Pressurization and heating-
The pressure and heating decomposition conditions are preferably a pressure of 40 to 200 MPa and a temperature of 40 to 80 ° C. for 1 to 36 hours, and a pressure of 50 to 150 MPa and a temperature of 50 to 70 ° C. for 3 to 24 hours.
If it is in the said decomposition condition range, the temperature which an enzyme reaction advances rapidly can be maintained, without raise | generating inactivation of an enzyme. Further, since the growth of harmful microorganisms can be prevented, there is no concern about corruption, and it is not necessary to add a preservative or other anti-corruption measures, which is preferable.
The pressurizing and heating conditions are not particularly limited and may be appropriately selected depending on the purpose, but are preferably performed using an enzyme fermentation accelerating device described later.

In the method for producing a cereal enzyme degradation product of the present invention, a saccharide-degrading enzyme and a proteolytic enzyme are added to cereal, and the reaction is performed at a pressure of 40 to 200 MPa and a temperature of 40 to 80 ° C. for 1 to 36 hours.
When pearl barley is used as the grain, it is preferable to add protease and amylase to the pearl barley and react at a pressure of 50 to 150 MPa and a temperature of 50 to 70 ° C. for 3 to 24 hours.
The pearl barley enzyme degradation product contains a large amount of cysteine, has a melanin production inhibitory action, an epidermal keratinocyte proliferation action, and a moisturizing action, and is suitably used as an active ingredient of foods and drinks, skin cosmetics, hair cosmetics, and bath preparations. Used.

There are no particular limitations on the apparatus used in the method for producing a grain enzyme degradation product of the present invention as long as it can satisfy the pressure and heating conditions as described above, and it is appropriately selected according to the purpose. For example, an enzyme fermentation promoting device as shown in FIG. 1 is suitable.
Here, FIG. 1 shows a schematic cross-sectional view of an enzyme fermentation promoting apparatus used in the method for producing a grain enzyme degradation product of the present invention. The enzyme fermentation promotion apparatus 1 includes a pressure vessel 2, a heater 3, a pressure pump 4, and a temperature sensor 5.

The pressure vessel 2, for example, the outer diameter _{L 1} is 100 mm, a cylindrical sealed container depth _{L 3} is 200mm wall thickness dimension _{L 2} is set to 150 mm. In this pressure-resistant container 2, what sealed the grain in the flexible container is put. Next, the pressure vessel 2 is filled with water. A heater 3 for heating is disposed on the outer peripheral surface of the pressure vessel 2. The heater 3 can raise the temperature in the pressure resistant container 2 to 80 ° C., and the inside of the pressure resistant container 2 can be set to an arbitrary temperature by operating an operation panel (not shown).

  In addition, a pressure pump 4 is connected to the pressure vessel 2, and the inside of the pressure vessel 2 can be adjusted to an arbitrary pressure of 0 to 200 MPa by operating the operation panel. . Further, a temperature sensor 5 and a pressure gauge 6 are attached to the pressure vessel 2. The temperature sensor 5 detects and displays the temperature in the pressure vessel 2. The pressure gauge 6 detects and displays the pressure in the pressure vessel 2.

  According to this enzyme fermentation accelerating device, it is possible to prevent spoilage, freely adjust the enzyme decomposition conditions (temperature, pressure, time), and it is not necessary to add preservatives. There is no need to add it, and it is possible to efficiently produce a grain enzyme degradation product having excellent functionality and safety.

(Functional goods)
The functional article of the present invention comprises the above-mentioned grain enzyme degradation product of the present invention, and further comprises other components as necessary. In this case, it is particularly preferable that the grain enzyme degradation product is a barley enzyme degradation product.
Examples of the functional article include foods and drinks, skin cosmetics, hair cosmetics, bathing agents, and the like.

-Food and drink-
The foods and drinks are those that are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life, such as foods, pharmaceuticals, quasi drugs, etc. It is not limited to this category, and means, for example, a wide range of foods that are orally ingested, including general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals.

  There is no restriction | limiting in particular as said food-drinks, Although it can select suitably according to the objective, For example, drinks, such as a soft drink, a carbonated drink, a nutrition drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, shaved ice Noodles such as buckwheat noodles, udon, harusame, gyoza skin, Chinese noodles, instant noodles, etc. , Confectionery such as bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red sea bream, scallop, abalone, sea urchin, sea bream, tocobushi, etc .; Fishery and livestock processed foods such as sausages; Dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise, show Oil and fat processed foods such as ning, whipped cream and dressing; seasonings such as sauces and sauces; curry, stew, oyakodon, rice bowl, miscellaneous cooking, Chinese rice bowl, and rice cake, tempura, eel rice, hayashi rice, oden, marvodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs; various forms of health nutrition supplements; pharmaceuticals such as tablets, capsules, drinks, troches; quasi drugs , Etc.

  The addition amount of the cereal enzyme degradation product of the present invention in the food or drink varies depending on the type of the food or drink to be targeted and cannot be unconditionally defined, but it is added within a range that does not impair the original taste of the food or drink. 0.001-50 mass% is preferable normally with respect to various object food-drinks, and 0.01-20 mass% is more preferable. Moreover, in the case of the food / beverage products of a granule, a tablet, or a capsule form, 0.01-100 mass% is preferable normally and 5-100 mass% is more preferable. In addition, about 1-1000 mg per day for an adult is suitable for the intake of cereal enzyme degradation products.

-Skin cosmetics and scalp cosmetics-
The skin cosmetic and scalp cosmetic comprise the grain enzyme degradation product of the present invention, and further comprise other components as necessary.
Examples of the other components include whitening agents, astringents, bactericides, antibacterial agents, ultraviolet absorbers, cell activators, anti-inflammatory agents, antiallergic agents, antioxidants, active oxygen scavengers, fats and oils, and waxes. , Hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, and the like.

The skin cosmetic is not particularly limited and may be appropriately selected from various uses. Examples thereof include ointments, creams, emulsions, lotions, packs, jellies, lip balms, lipsticks, bath preparations, and astringents. .
There is no restriction | limiting in particular as said scalp cosmetics, It can select suitably from various uses, For example, hair tonic, hair cream, hair liquid, shampoo, pomade, rinse, etc. are mentioned.

  The blending amount of the cereal enzyme decomposition product with respect to the skin cosmetic or scalp cosmetic can be appropriately adjusted according to the type of the skin cosmetic or scalp cosmetic, but is preferably 0.0001 to 10% by mass. .

  The grain enzyme degradation product and functional article of the present invention are suitably applied to humans, but should be applied to animals other than humans as long as each effect is exhibited. You can also.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Production Example 1)
-Manufacture of pearl barley enzyme degradation product
Water (200 mL), protease (1 g), and amylase (0.3 g) were added to 100 g of pearl barley powder, and reacted at 50 ° C. and 60 MPa for 24 hours using the enzyme fermentation promotion apparatus (manufactured by Yanmar Co., Ltd.) shown in FIG. 500 mL of water was added to the obtained reaction solution, and filtration was performed with diatomaceous earth. The obtained filtrate was concentrated under reduced pressure to obtain 48.7 g of pearl barley enzyme degradation product.

(Production Example 2)
-Production of wheat enzyme degradation product-
200 g of water, 1 g of protease, and 0.3 g of amylase were added to 100 g of wheat powder, and reacted at 50 ° C. and 60 MPa for 24 hours using the enzyme fermentation promotion apparatus (manufactured by Yanmar Co., Ltd.) shown in FIG. 500 mL of water was added to the obtained reaction solution, and filtration was performed with diatomaceous earth. The obtained filtrate was concentrated under reduced pressure to obtain 36.5 g of a wheat enzyme degradation product.

Example 1
-Melanin production inhibition test-
After culturing B16 melanoma cells using Dulbecco MEM containing 10% by mass FBS, the cells were collected by trypsin treatment. The collected cells were diluted with Dulbecco MEM containing 10% by mass FBS and 0.5 mmol / L theophylline to a concentration of 8.0 × 10 ⁵ cells / mL, and then 10% by mass FBS and 0.5 mmol / L theophylline. 0.5 mL each was seed | inoculated to the petri dish with a diameter of 60 mm which added 2 mL of containing Dulbecco MEM, and it culture | cultivated for 8 hours.
Next, 2.5 mL of the test sample of Production Example 1 prepared to double the final concentration with 10% by mass FBS and 0.5 mmol / L theophylline-containing Dulbecco MEM was added and cultured for 4 days. After completion of the culture, cells were collected by trypsin treatment and counted. Subsequently, the medium was removed by centrifugation (2500 × g, 6 minutes, room temperature), and 2 mL of a 1 mol / L sodium hydroxide solution containing 10% by mass DMSO was added, and the cells were disrupted with an ultrasonic crusher. This was filtered, and the absorbance of the obtained filtrate at a wavelength of 475 nm was measured.
The calculation method of the melanin production inhibition rate is as shown in the following formula 1.

<Formula 1>
Melanin production inhibition rate (%) = [(A−B) / A × (C / D)] × 100
However, in the said Numerical formula 1, A represents the light absorbency in 475 nm without a test sample addition. B represents the absorbance at 475 nm upon addition of the test sample. C represents the number of cells when the test sample is added. D represents the number of cells with no test sample added.

Next, the concentration of the sample solution is decreased stepwise to measure the inhibition rate, and the inhibition rate (%) of melanin production at each concentration is determined. From the result, the production of melanin is inhibited by 50%. The sample concentration IC ₅₀ (ppm) was determined. The results are shown in Table 1.

(Example 2)
-Epidermal keratinocyte proliferation test-
Normal human neonatal foreskin keratinocytes (NHEK) were cultured using a growth medium for long-term culture of normal human epidermal keratinocytes, and then cells were collected by trypsin treatment. The collected cells are diluted with a growth medium for normal human epidermal keratinocyte long-term culture to a concentration of 1.5 × 10 ⁴ cells / mL, then seeded at 100 μL per well in a collagen-coated 96-well plate, and cultured overnight. did. After completion of the culture, 100 μL of the test sample of Production Example 1 dissolved in a growth medium for long-term culture of normal human epidermal keratinocytes was added to each well and cultured for 3 days.
Next, epidermal keratinocyte proliferation action was measured using MTT assay. After completion of the culture, the medium was removed, and 100 μL of MTT dissolved in PBS (−) at a final concentration of 0.4 mg / mL was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced.
The calculation method of the epidermal keratinocyte proliferation promotion rate is as shown in the following formula 2.

<Formula 2>
Epidermal keratinocyte proliferation promotion rate (%) = (St / Ct) × 100
However, in the numerical formula 2, St represents the absorbance in the cells to which the test sample is added. Ct represents the absorbance in cells to which no test sample is added.

  Next, Table 2 shows the epidermal keratinocyte proliferation promotion rate (%) at sample concentrations of 12.5 μg / mL and 3.125 μg / mL.

表２中、Ｍｅａｎ±Ｓ．Ｅ．、ｎ＝６、＊：ｐ＜０．０５、＊＊：ｐ＜０．０１、＊＊＊：ｐ＜０．００１を表す。

In Table 2, Mean ± S. E. , N = 6, **: p <0.05, **: p <0.01, ***: p <0.001.

(Example 3)
-Moisturizing test-
First, (1) 1 g of pearl barley enzyme degradation product of Production Example 1, 10 g of 1,3-butylene glycol, and a sample whose remaining amount was 100 g with purified water, (2) 1% by mass glycerin solution, (3) purified water, Prepared.
Next, 50 μL of each of the sample solutions (1) to (3) was dropped onto a paper disk having a diameter of 8 mm (manufactured by Toyo Seisakusho, mass 0.017 g). This was left in the test chamber and the mass after 0 to 10 minutes was measured every 2 minutes. The water residual ratio of each sample solution was determined with the mass at 0 minute being 100%. The results are shown in Table 3. The room temperature in the test room was 25 ° C. and the humidity was 65% RH.

  From the results of Tables 1 to 3, it was found that the pearl barley enzyme degradation product of Production Example 1 has excellent melanin production inhibitory action, epidermal keratinocyte proliferation action, and moisturizing action.

Example 4
A cream having the following composition was produced by a conventional method.
Pearl barley enzyme degradation product (Production Example 1) 0.01 g
Rosemary extract 0.1g
Hamamelis extract 0.1g
3.0g polyglyceryl condensed ricinoleate
Squalane 8.0g
Macadamia nut oil 3.0g
Glyceryl tri-2-ethylhexanoate 5.0 g
4.0 g of methylphenylpolysiloxane
Sodium chloride 0.5g
Preservative (Propyl paraoxybenzoate) 0.1g
Perfume appropriate amount 1,3-butylene glycol 5.0 g
Glycerin 3.0g
Purified water balance
Total amount 100g

(Example 5)
A serum having the following composition was produced by a conventional method.
Pearl barley enzyme degradation product (Production Example 1) 0.02 g
Carrot extract 0.1g
Oil soluble licorice extract 0.1g
Royal jelly extract 0.1g
Peach leaf extract 0.1g
Yeast extract 0.1g
Xanthan gum 0.3g
Hydroxyethylcellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance Appropriate amount Preservative (methyl paraoxybenzoate) 0.15g
Ethanol 2.0g
Purified water balance
Total amount 100g

(Example 6)
A pack having the following composition was produced by a conventional method.
Pearl barley enzyme degradation product (Production Example 1) 0.001 g
0.1g dipotassium glycyrrhizinate
Polyvinyl alcohol 15.0g
Carboxymethylcellulose 5.0g
Glycerin 3.0g
Ethanol 10.0g
Fragrance 0.5g
Preservative (butyl paraoxybenzoate) appropriate amount Antioxidant (tocopherol acetate) appropriate amount
Purified water balance
Total amount 100g

(Example 7)
The following raw material A was dissolved in 70 g of purified water at 60 ° C. A mixture of the following raw material B was added thereto, stirred and cooled to produce a hair tonic.
<Raw material A>
Pearl barley enzyme degradation product (Production Example 1) 0.5 g
Resorcin 0.01g
0.1g dipotassium glycyrrhizinate
Carrot extract 0.5g
<Raw material B>
0.1 g of pyridoxine hydrochloride
D-pantothenyl keel 0.1g
L-Menthol 0.05g
1,3-butylene glycol 4.0 g
Perfume proper amount Ethanol 25.0g

(Example 8)
After dissolving the barley enzyme degradation product of Production Example 1 in 1,3-butylene glycol, the remaining raw materials were directly added to 70 g of purified water to prepare an aqueous solution of raw material A. The following mixture of raw material B was added thereto and stirred to produce a hair lotion.
<Raw material A>
Pearl barley enzyme degradation product (Production Example 1) 0.5 g
Jamane Mandro Extract 0.5g
1,3-butylene glycol 4.0 g
Dipotassium glycyrrhizinate 0.2g
Oleyl alcohol 4.0g
Preservative appropriate amount <Raw material B>
Polyoxyethylene sorbitan monolaurate (20EO) 1.5g
Polyoxyethylene lauryl ether (20EO) 0.5g
Perfume proper amount ethanol 15.0g

Example 9
The following raw material B was added to 40 g of purified water and heated to 70 ° C. to dissolve. The mixture of the following raw material A heated at 70 degreeC was added there, and it emulsified using the homogenizer, and manufactured the hair cream.
<Raw material A>
Pearl barley enzyme degradation product (Production Example 1) 0.5 g
Stearyl glycyrrhetinate 0.1g
Bead wax 10.0g
Cetanol 5.0g
Hydrophilic laurin 8.0g
Squalane 37.5g
Glycerol monostearate 2.0g
γ-Oryzanol 0.05g
<Raw material B>
Polyoxyethylene sorbitan monolaurate (20EO) 2.0g
Polyethylene glycol 5.0g
Preservative appropriate amount Fragrance proper amount

(Example 10)
A shampoo having the following composition was produced by a conventional method.
Pearl barley enzyme degradation product (Production Example 1) 0.1 g
Lauryl sulfate triethanolamine 5.0 g
Sodium polyoxyethylene lauryl ether sulfate 12.0g
Lauric acid diethanolamide 2.0g
Edetate disodium 0.1g
1.3-Butylene glycol 4.0 g
Methyl paraoxybenzoate 0.15g
Fragrance 0.05g
Purified water balance
Total amount 100g

(Example 11)
A rinse having the following composition was produced by a conventional method.
Pearl barley enzyme degradation product (Production Example 1) 0.1 g
Stearyltrimethylammonium chloride 2.0g
Cetostearyl alcohol 2.0g
Polyoxyethylene lanolin ether 3.0g
Methyl paraoxybenzoate 0.15g
Propylene glycol 5.0g
Fragrance 0.05g
Purified water balance
Total amount 100g

(Example 12)
The following mixture was tableted to produce a tablet-shaped dietary supplement.
Pearl barley enzyme degradation product (Production Example 1) 50 g
Powdered sugar (sucrose) 188g
Glycerin fatty acid ester 12g

(Example 13)
The following mixture was tableted to produce a tablet-shaped dietary supplement.
Wheat enzyme degradation product (Production Example 2) 50 g
Powdered sugar (sucrose) 188g
Glycerin fatty acid ester 12g

(Example 14)
The following mixture was formed into granules to produce a granular dietary supplement.
34g of pearl barley enzyme degradation product (Production Example 1)
1000g beet oligosaccharide
Vitamin C 167g
Stevia extract 10g

(Example 15)
The following mixture was formed into granules to produce a granular dietary supplement.
34 g of wheat enzyme degradation product (Production Example 2)
1000g beet oligosaccharide
Vitamin C 167g
Stevia extract 10g

(Example 16)
According to the following prescription, the drink was manufactured by the conventional method.
Pearl barley enzyme degradation product (Production Example 1) 3 g
Glucose sucrose fructose 10g
Citric acid 1g
Sodium citrate 0.5g
Fragrance 0.01g
0.01g of dye
Purified water balance
Total amount 100g

(Example 17)
According to the following prescription, the drink was manufactured by the conventional method.
Wheat enzyme degradation product (Production Example 2) 3g
Glucose sucrose fructose 10g
Citric acid 1g
Sodium citrate 0.5g
Fragrance 0.01g
0.01g of dye
Purified water balance
Total amount 100g

  The cereal oxygen degradation product of the present invention is obtained by treating cereal with a plurality of enzymes under pressure and heating, and has a useful function. For example, food and drink, skin cosmetics, hair cosmetics, Widely used in bathing agents.

FIG. 1 is a schematic cross-sectional view showing an example of the enzyme fermentation promoting device of the present invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Enzyme fermentation promotion apparatus 2 Pressure-resistant container 3 Heater 4 Pressure pump 5 Temperature sensor 6 Pressure gauge

Claims (6)

Two kinds of enzymes with different substrate specificities and reaction speeds of amylase and glucosidase carbohydrase and protease and peptidase proteolytic enzyme against barley under pressure and heating An epidermis keratinocyte proliferation promoter comprising a barley enzyme degradation product obtained by reacting with The epidermal keratinocyte proliferation promoter according to claim 1, wherein the enzyme is reacted at a pressure of 40 MPa to 200 MPa and a temperature of 40 ° C to 80 ° C for 1 hour to 36 hours. The epidermal keratinocyte proliferation promoter according to claim 2, wherein the enzymes are protease and amylase, and the enzymes are reacted at a pressure of 50 MPa to 150 MPa and a temperature of 50 ° C to 70 ° C for 3 hours to 24 hours. A skin cosmetic comprising the epidermal keratinocyte proliferation promoter according to any one of claims 1 to 3. A hair cosmetic comprising the epidermal keratinocyte proliferation promoter according to any one of claims 1 to 3. A bath preparation comprising the epidermal keratinocyte growth promoter according to any one of claims 1 to 3.

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