JP2010180193A - High-quality cosmetic - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To newly develop a cosmetic markedly enhanced in cosmetic benefits inherent of a placenta extract (especially skin whitening effect, moisturizing effect, and anti-oxidative effect) and having high safety. <P>SOLUTION: The problem is solved by a cosmetic prepared by enclosing a placenta extract and a Coix lacryma-jobi seed powder in liposomes. An example of the cosmetic is as follows. A lipid film component comprising a phospholipid and/or a glycolipid is dissolved in a solvent, and a water phase containing a water-soluble placenta extract and a Coix lacryma-jobi seed extract is added to the solution under agitation. The resultant mixture is subsequently heated and agitated to disperse the lipid component to form fine liposomes. The liposomes, which have an average particle diameter of 100 nm or smaller, are separated and recovered to obtain the placenta extract/Coix lacryma-jobi seed powder-enclosed liposomes. These are used as a raw cosmetic material to produce various cosmetics. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

The present invention relates to high-quality cosmetics, particularly liposomal cosmetics containing placenta and pearl barley as active ingredients. In particular, the present invention relates to a novel cosmetic comprising encapsulating placenta and pearl barley extracts in liposomes. More specifically, by combining placenta and pearl barley extract and encapsulating them in liposomes, the effects of placenta alone, in particular, whitening, moisturizing, and antioxidant effects are significantly improved, and highly safe cosmetics. About. Moreover, the present invention relates to a cosmetic that not only has excellent medicinal effects but also provides a feeling that it is comfortable to use and penetrates deep into the skin. Particularly in skin cosmetics, not only medicinal effects but also comfort (feeling of cosmetic use) are important properties and factors.

  Conventional whitening effect for skin lotion, milky lotion and other cosmetics to improve skin darkening and pigmentation spots, freckles, etc., as well as moisturizing and antioxidant effects to prevent spots and sagging And various raw materials are blended in cosmetics.

  As examples, whitening agents include ascorbic acid derivatives, glutathione, hydroquinone, etc., moisturizers such as collagen, hyaluronic acid, ceramide, etc., and antioxidants such as vitamin E as raw materials (Patent Document 1).

  However, these raw materials have only a single beauty effect, and in the case of imparting a plurality of beauty effects to a single cosmetic, etc., it is necessary to blend raw materials having various different effects into cosmetics, etc. In addition, the complexity of the prescription in the production process has been a problem, and it has been suggested that the use of a plurality of raw materials at the same time may inhibit the effects of the individual raw materials.

  As a raw material that can solve such problems, placenta extract (placenta extract) has attracted attention. Placenta has been used as a traditional Chinese medicine since ancient times, and in recent years, placenta extract, which extracts active ingredients from placenta, has been widely used in the fields of medicine and beauty. Especially in the field of beauty, it is directly introduced into blood vessels by injection or infusion. And a method of external application to the skin, a method of percutaneous ingestion by iontophoresis, or a supplement or health food by ingestion orally.

  Placenta extract ingested in this way has endocrine regulating, strong liver / detoxifying, immunostimulatory, autonomic nervous, blood circulation / hematopoietic effects, and cosmetic aspects of metabolism. It has many functions such as promotion, improvement of rough skin by anti-inflammatory action, whitening effect by antioxidant action, and recovery of skin aging, and these effects can be obtained when used alone. Therefore, it is extremely useful as a raw material for beauty cosmetics.

However, although placenta extract has very many functions as described above, there is a problem that each effect is inferior to other components. For example, when focusing on the whitening effect of placenta extract, Cosmetics as an active ingredient are not sufficiently effective compared to cosmetics containing the aforementioned ascorbic acid derivative as an active ingredient. Furthermore, unlike injection and infusion introduced directly into blood vessels, the percutaneous ingestion of placenta extract does not acquire an efficiency sufficient to exert its effect.

JP 2003-267817 A

  The present invention is characterized by remarkably improving the efficacy of placenta extract, in particular, whitening, moisturizing and antioxidant effects, in order to solve the above-mentioned problems, yet being highly safe and easy to formulate. The purpose is to provide cosmetics. In addition, since the present invention is intended for cosmetics, particularly skin cosmetics, it is also intended to improve usability and percutaneous absorption.

  The present invention has been made to achieve the above object. However, since placenta extract is a complex natural product-derived component containing various components, the original action of placenta extract is often reduced or even disappears in some cases when it is mixed with other components.

  In consideration of this point, the present inventors conducted careful examinations from various directions, and as a result of extensive screening, it was totally unexpected that when placenta extract was blended, placenta extract originally possessed In addition to various effects (various pharmacological actions) that have not been spoiled at all, the present inventors have obtained a new and extremely useful new finding that the effects (pharmacological action) possessed by pearl barley extract are further exhibited.

  However, as a result of further research without satisfying the above useful new findings, the present inventors have encapsulated placenta extract and pearl barley in liposomes. It was further found out that (property, action) can be obtained.

  In that case, placenta extract and pearl barley are encapsulated in liposomes individually and mixed, and when both are encapsulated in liposomes, the usability is further improved. I found out for the first time.

  Based on these useful new findings, the present invention has finally been achieved as a result of further research, and is a basic technical idea of cosmetics (cosmetics, external preparations for skin) containing liposomes encapsulating placenta and pearl barley. And

  Examples of the embodiment of the present invention are as follows.

(1) A lipid membrane component composed of phospholipid and / or glycolipid and a solvent are mixed and stirred, and an aqueous phase containing a water-soluble placenta extract and pearl barley powder is added to the obtained liquid and stirred. The liquid was heated and stirred, and then dispersed to form fine liposomes. After cooling to room temperature or without cooling, liposomes having an average particle size of 100 nm or less were separated and collected using a sieve. Obtaining placenta / barley-encapsulated liposome solution and producing cosmetics using the placenta / elder-encapsulated liposomes prepared in this way, improving transdermal absorbability, improving the feeling of use, and improving the comfort of use A method for producing a cosmetic that exhibits at least one of the above-described effects.
(2) The method according to (1), wherein a placenta extract is used as the water-soluble placenta extract.
(3) As a barley powder, it is obtained by adding water to the coarsely ground powder without peeling off the shell of the barley or adding the enzyme having protease activity and the enzyme having α-amylase activity. The method according to (1) or (2), characterized by using a processed product (barley extract) or a powdered product thereof (barley extract powder).
(4) The average particle diameter is 75 nm or less and is as small as possible, for example, 10 to 60 nm, preferably 15 to 50 nm, more preferably 20 to 30 nm. The method according to any one of (1).
(5) The method according to any one of (1) to (4), wherein the cosmetic is a skin cosmetic.
(6) The method according to any one of (1) to (5), wherein the cosmetic has at least one of a whitening effect, a moisturizing effect, and an antioxidant effect.

(7) A cosmetic comprising liposomes encapsulating placenta and pearl barley, produced by the method according to any one of (1) to (6).
(8) It has at least one effect of whitening effect, moisturizing effect and antioxidant effect, and further has at least one effect of improving transdermal absorbability, improving the feeling of use and improving the feeling of use. The cosmetics described.
(9) The cosmetic is at least one of lotion, cosmetic liquid, milky lotion, cream, pack, mask, bathing agent, cleaning agent, dispersion liquid, ointment, liquid agent, aerosol, and patch. Cosmetics as described in (7) or (8).
(10) A non-therapeutic comfortable cosmetic method characterized by applying the cosmetic according to any one of (6) to (9) to the skin locally and / or systemically (for non-therapeutic use) A cosmetic and comfortable treatment method).
However, “comfortable” means at least one of the following (a) to (c).
(A) Improvement of transdermal absorbability (feeling of penetration) (b) Improvement of feeling of use (c) Improvement of feeling of use (11) The cosmetic according to any one of (6) to (9) is applied to the skin Non-therapeutic as described in (10), characterized by exerting (showing) at least one effect of whitening, moisturizing, and antioxidant on the skin by being applied locally and / or systemically Comfortable makeup method.

  The present invention relates to a cosmetic comprising a liposome encapsulating placenta and pearl barley. Placenta extract has excellent effects such as whitening effect, but since it is a complex raw material that is derived from natural products and contains a large number of various components, other components (raw materials) ) In combination (combined use) and the placenta's original effect is reduced, and in some cases, it can be sufficiently predicted that the effect will be canceled out.

  However, according to the present invention, by adding (combined) pearl barley extract, the effect of the placenta is not only played as it is, but the effect is further enhanced, and the feeling of roughness and rough skin is increased. The effect far exceeds the prediction that effects such as reduction will be achieved.

  Therefore, according to the present invention, as will be apparent from the following description, not only an excellent whitening effect, a moisturizing effect and an antioxidant effect are exhibited, but the detailed mechanism must be revisited in the future, In combination with the encapsulation of active ingredients (placenta, pearl barley) in liposomes, effects specific to cosmetics, particularly skin cosmetics, such as improved transdermal absorbability, improved usability, and improved comfort are also exhibited. In cosmetics, it is not necessary to say that pharmacological effects such as whitening effects are important, but sensual effects such as comfort and tightness are also extremely important. The present invention is not only excellent in pharmacological effects and sensory effects, but also excellent in that both effects can be achieved simultaneously.

  In addition, the effect exhibited by the present invention is that the liposome encapsulating a mixture of placenta and pearl barley is superior to the case of mixing the liposome encapsulating placenta and the pearl barley encapsulating, particularly in the sensory effect. This is also very characteristic. That is, according to the present invention, in addition to the above pharmacological effects, the percutaneous absorbability is improved, the permeation of cosmetics is improved, a pleasant feeling of use is obtained, the skin is shiny and smooth, and the foundation is smooth, In addition, there is a remarkable effect that there is no skin irritation.

The tyrosinase activity inhibition when each component is used alone or in combination is shown. The DPPH radical scavenging activity when each component is used alone or in combination is shown. The particle diameter of the liposome measured with a particle size distribution analyzer is shown.

  Hereinafter, the present invention will be described in detail, but the following description is one of preferred specific examples of the present invention, and the present invention is not limited to these descriptions.

  The active ingredients of the present invention are placenta and pearl barley. The placenta is appropriately selected from placenta derived from mammals such as humans, pigs, sheep, cows and horses, or so-called plant placenta derived from plants such as soybeans, aloe vera, and kakon. A placenta extract (also called placenta extract, placenta extract, placenta solution, water-soluble placenta extract, etc.) is used. As the placenta extract, commercially available products can be used as appropriate.

  As the pearl barley, a processed product of pearl barley belonging to the gramineous plant is used. For example, pearl barley powder is preferably used.

  As for the pearl barley powder, 2-10 times the weight ratio of water is added to the crushed crushed shell of the pearl barley without removing the shell, and the enzyme having protease activity and the enzyme having α-amylase activity are added. The processed product (pearl barley extract) obtained by adding the above, or the powdered product (barley extract powder) can be used.

  The pearl barley is coarsely pulverized without peeling off the shell or peeled off, added with water, and then heated to 85 ° C. to 90 ° C. or higher (eg, 95 to 100 ° C.) for 5 to 90 minutes, preferably 10 to 50 minutes. Leave for 20-40 minutes. Subsequently, it cools to 40-65 degreeC, Preferably it is 50-60 degreeC, and the enzyme which has protease activity and the enzyme which has alpha-amylase activity are added simultaneously, or it reacts separately, respectively. Although depending on the type and concentration of the enzyme, the reaction time (treatment time) is usually preferably about 30 to 90 minutes.

  After completion of the reaction, the enzyme is deactivated by heating to, for example, 80 to 100 ° C. Next, a residue is removed, heat-sterilized (for example, 80-100 degreeC), and the obtained liquid is made into a pearl barley processed material (barley extract). By applying a drying means such as spray-drying or freeze-drying as it is without adding any additives to this pearl barley extract, a powder (barley extract powder) is obtained. The pearl barley extract may be concentrated as needed, or may be diluted on the contrary. In any case, in the present invention, pearl barley extract and pearl barley extract powder are collectively referred to as pearl barley powder, and both are used in combination or are used singly.

  As the enzyme having protease activity, proteolytic enzymes are widely used. For example, endopeptidase, exopeptidase, aminopeptidase, dipeptidase, carboxypeptidase and the like are used. As the enzyme having α-amylase activity, amylolytic enzymes are widely used, and for example, α-amylase, β-amylase, glucoamylase, and the like are used singly or in combination.

The pearl barley powder is produced, for example, as follows.
It is preferable to prepare 1 ton of pearl barley (fruit part), remove the shell and then coarsely pulverize (unpolished one such as Yokuinin etc. For example, K, Mg, P, Fe , Zn, Cu, Vitamin B ₁ , B _2, etc., the content of unpolished one is much higher.), 4 times the amount of coarsely ground pearl barley is added, heated to 100 ° C. and allowed to stand for 30 minutes To do. Next, the temperature is adjusted to 55 ° C. with cooling water, and 9.4 g and 640 g of protease and α-amylase dissolved in appropriate amounts of water are added and reacted for 1 hour. Then, it heats to 90 degreeC and inactivates an enzyme. The residue is removed, and the liquid after heat sterilization at 90 ° C. is obtained as pearl barley extract. The pearl extract is pulverized by spray drying without adding additives to obtain pearl extract powder.

  For example, the liposome is prepared as follows. First, a lipid membrane component (phospholipid and / or glycolipid) and, if necessary, a lipophilic medicinal component are added and stirred and dissolved. Moreover, in order to improve the solubility of phospholipid etc. as needed, the solvent accept | permitted by cosmetics etc. can also be used. Subsequently, an aqueous phase containing placenta and pearl barley is continuously added, stirred and dispersed to form fine liposomes.

  The mixture of placenta extract and pearl barley thus obtained was encapsulated in liposomes by a conventional method, and as a method, mechanical vibration method, sonication method, reverse phase evaporation method, freeze-drying method, heating method, Examples of the method include a polyhydric alcohol method, a mechanochemical method, a lipid dissolution method, and a spray drying method, but are not limited to these methods. Moreover, the shape of the liposome produced using these methods is not limited to either a single liposome or a multilamellar liposome. Furthermore, it is possible to separate and select liposomes having an appropriate particle size by appropriately passing through a filter, but the average particle size is 200 nm or less, preferably 150 to 100 nm or less, more preferably 75 nm or less. For example, 10 to 60 nm, preferably 15 to 50 nm, more preferably 20 to 30 nm (for example, 25 nm).

  The liposome of the present invention may be any liposome that is generally known as a liposome, and in particular, liposomes that have no problem when applied to the skin. These are used alone or by using known liposome materials. Liposomes may be designed and formed. More preferably, phospholipids are used. As the phospholipids, those generally known as phospholipids including natural phospholipids and synthetic phospholipids can be used. For example, soybean, egg yolk-derived lecithin, lysolecithin, or derivatives thereof such as hydrogenated products and hydroxides thereof are exemplified. In this case, the constituent phospholipid is not particularly limited, and the constituent fatty acid is not particularly limited, and may be either a saturated fatty acid or an unsaturated fatty acid.

  Specific examples of the phospholipid used include phosphatidylcholine (lecithin), phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, phosphatidylethanolamine, sphingomyelin, phosphatidic acid, and more specific examples include dipalmitoylphosphatidylcholine. (DPPC), distearoylphosphatidylcholine (DSPC), dimyristolphosphatidylcholine (DMPC), dioleylphosphatidylcholine (DOPC), dipalmitoylphosphatidylglycerol (DPPG), distearoylphosphatidylserine (DSPS), distearoylphosphatidylglycerol (DSPG), Dipalmitoylphosphatidylinositol (DPPI), distealoy Phosphatidylinositol (DSPI), dipalmitoyl phosphatidic acid (DPPA), etc. distearoyl lysophosphatidic acid (DSPA) can be exemplified. By adding 0.1 to 20 (w / w)%, preferably 0.5 to 10 (w / w)% of the total liposome raw material, the liposome can be obtained without any problem.

  Specifically, as glycolipids, glycerolipids such as digalactosyl diglyceride and galactosyl diglyceride sulfate, and glycosphingolipids such as galactosylceramide, galactosylceramide sulfate, lactosylceramide, ganglioside G6, and ganglioside G4 can be preferably used.

  In order to improve the solubility of the lipid membrane component, it is preferable to use a solvent. As the solvent, it is preferable to use one or more of lower alcohols, glycols, glycol ethers, glycol esters, and alkyl carbonates.

  Preferred lower alcohols include methanol, ethanol, isopropanol and the like, and ethanol is particularly desirable from the viewpoint of safety.

  Preferred glycols, glycol ethers, glycol esters include ethylene glycol, propylene glycol, dipropylene glycol, 1,3-butanediol, ethyl carbitol, butyl carbitol, diethylene glycol diethyl ether, alkyl esters of these glycols, and the like. .

  Preferred alkyl carbonates include alkyl carbonates such as propylene carbonate and diethyl carbonate.

  The liposome of the present invention can be suitably used for cosmetics and skin external preparations by encapsulating a hydrophilic medicinal component and / or a lipophilic medicinal component. Suitable medicinal ingredients include antioxidants, antibacterial agents, anti-inflammatory agents, blood circulation promoters, whitening agents, rough skin prevention agents, anti-aging agents, moisturizers, hormone agents, vitamins, pigments, and proteins. . Further, auxiliary agents commonly used in cosmetics and preparations for external use on the skin can also be used as appropriate.

  Suitable antioxidants include tocopherols, natural vitamin Es and their fatty acid esters, BHT, BHA, hydroxyanisole, t-butylhydroquinone, nordihydroguaiaretin, gallic acid alkyl esters, thiodipropionic acid Dilauryl, tolylbiguanide, sodium bisulfite, sodium sulfite, sodium pyrosulfite, erythorbic acid and its salts, L-ascorbic acid and its salts, fatty acid ascorbyls, tea extracts, apple extracts and other catechins, catechin derivatives, and polyphenols And the like. Also preferred are amino acids having a reducing group such as thiotain, glutathione, thiotaurine, hypotaurine.

  Suitable antibacterial agents include organic acids such as benzoic acid, undecylenic acid, salicylic acid, sorbic acid, dehydroacetic acid, alkyl valoxybenzoate and salts and derivatives thereof, isopropylmethylphenol, chlorhexidine, chlorhexidine gluconate, phenol, cresol And phenols such as thymol and triclosan, hinokitiol, hiba oil, tea extract, apple extract, plant extract substances, catechins, catechin derivatives, and polyphenols. Also preferred are chloramine T, zinc pyrithione, and the like.

  Suitable anti-inflammatory agents include ε-aminocaproic acid, tranexamic acid, tranexamic acid alkyl ester, glycyrrhizic acid, glycyrrhizic acid alkyl ester, glycyrrhetinic acid, glycyrrhetinic acid alkyl ester, lysozyme chloride, guaiazulene, hydrocortisone, perilla extract, ginkgo biloba extract, seaweed Examples include plant extracts such as extracts.

  Suitable blood circulation promoters include plant extracts such as ginkgo biloba extract, assembly extract, sericoside, and maronier extract, tocopherols, vitamin E and its derivatives, and γ-oryzanol.

  Suitable whitening agents include L-ascorbic acid, sodium L-ascorbate, sodium ascorbate phosphate, magnesium ascorbate phosphate, sodium ascorbate sulfate, glyceryl ascorbate (polyglyceryl) ethers, glyceryl ascorbate (Polyglyceryl) esters, ascorbic acid glucosides (glycosides), ascorbic acid alkyl esters, ascorbic acid alkyl ethers and other vitamin C and derivatives thereof, kojic acid, alptin, sulfur and the like.

  In addition, plant extracts such as oil-soluble licorice extract, mulberry extract, peony extract, pearl millet extract, bitumen extract, maronier bark extract and chamomile extract, and linoleic acid, linolenic acid, lactic acid, tranexamic acid and the like are also preferred.

  Suitable skin roughening agents and moisturizers include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, polyhydric alcohols such as sorbitol, saccharides such as trehalose and maltose, sodium hyaluronate, chondroitin sulfate, chitin Examples thereof include biopolymers such as chitosan, amino acids such as sodium pyrrolidonecarboxylate and hydroxyproline, sodium lactate, urea, sphingolipids, ceramides, cholesterol and its fatty acid esters, and polyoxyethylene adduct derivatives. In addition, hydrocarbon oils such as squalane and squalene, and glycerides such as kukui nut oil, borage oil, and rosehip oil can also be suitably used.

  Suitable antioxidants include the above-mentioned antioxidants, humectants, blood circulation promoters, and rough skin inhibitors. Further, α-hydroxy acid, vitamin A and its derivatives, vitamin A alcohol and its derivatives can be preferably used.

  Suitable hormonal agents include steroid hormones such as corticosterone, andosterone, hydrocortisone, β-estradiol, prostaglandins and the like.

  Suitable vitamins include one or more selected from vitamin A group, vitamin B group, vitamin C group, vitamin E group, vitamin K group, flavonoids, carotenoids, quinones, A dye such as porphyrin can also be used.

  The concentrations of the placenta extract and pearl barley extract encapsulated in the liposome can be determined as appropriate, but preferably the concentration of the placenta extract is 5 to 50 (w / w)% of the total liposome raw material and the pearl barley extract powder is 1 to 30 (w / w)% is dissolved, and more preferably 1 to 25 (w / w)% pearl barley extract powder is dissolved in 20 to 40 (w / w)% placenta extract.

  The liposome of the present invention can be used as a cosmetic or a skin external preparation as it is or by mixing it with an existing preparation. In the case of mixing with an existing preparation, it may be mixed at the final stage of the preparation, or may be mixed during the preparation, for example, when an aqueous phase is added. As the mixing conditions and other conditions, the conditions for producing normal cosmetics and external preparations for skin can be applied as they are. These various preparations are known as commonly used in the technical field of preparations of excipients, binders, disintegrants, lubricants, solubilizers, suspensions and other cosmetics and external preparations for the main agent (liposomes). It can be formulated using adjuvants. In addition, as described above, a medicinal component such as an antibacterial agent that can be used for preparing liposomes can be appropriately blended.

  The liposome encapsulating the medicinal component according to the present invention is kept stable even when mixed with an existing preparation, and the stability of the medicinal component is not impaired.

  In the present invention, the cosmetic refers to at least one of cosmetics, cosmetic compositions, and external preparations for skin, and is not particularly limited as long as it is generally acceptable, but specifically includes the following: Lotion, cosmetic liquid, milky lotion, cream, pack, mask, bathing agent, cleaning agent, dispersion liquid, ointment, liquid agent, aerosol, patch.

  Hereinafter, although the test example and Example of this invention are described, this invention is not limited only to these.

(Test Example 1: Whitening effect improvement test)
Black-brown melanin, which causes dark spots and skin darkening, is produced by the action of an enzyme called tyrosinase on an amino acid called tyrosine. Therefore, in order to maintain white and clear skin, the activity of tyrosinase must be inhibited. Details of the experiment conducted to verify the whitening effect of the mixed solution of placenta and pearl barley used in the present invention are described below.

(1) Experiment As a test sample, a placenta extract solution (Nichirei Bioscience Products Co., Ltd.) is used as a water-soluble placenta extract, and as a barley powder, a barley extract is produced according to the method described above and further sprayed. The dry barley extract powder was used. These may be referred to as placenta solution and pearl barley extract powder, respectively.

  In the test, the mushroom-derived tyrosinase (Sigma) calculated the absorbance measurement value in units of 0.1 seconds of the initial reaction rate at which dopachrome was generated, using kinetics software, and mixed the placenta solution, barley extract powder and the samples. The inhibition rate of tyrosinase activity was determined by comparing with the initial reaction rate when the product was added. The tyrosinase activity inhibition rate was calculated from the average value measured five times.

  The experimental procedure is as follows. 40 μL of 3 mM L-DOPA (Wako Pure Chemical Industries), 20 μL of 0.2 M phosphate buffer (pH 6.5) and 40 μL of water (control) or each sample (25 (v / v))% placenta solution, 1 mg ( 2.5 wt%) barley extract powder or 25% placenta solution + 1 mg barley extract powder), let stand at 37 ° C. for 10 minutes, add 20 μL of 300 unit / mL tyrosinase solution, and calculate absorbance change at 475 nm Measurements were taken in 2-5 seconds. The results are summarized below.

(2) Results As is clear from the results of FIG. 1, the tyrosinase inhibition rates of the 25% placenta solution and 1 mg pearl extract powder were 25.3% and 14.4%, respectively. On the other hand, the tyrosinase inhibition rate of the 25% placenta solution + 1 mg pearl extract extract showed the strongest tyrosinase inhibition rate, which was 39.7%. The placenta solution and pearl barley extract mixed solution showed stronger tyrosinase inhibition rates than the single samples, respectively, indicating the effectiveness of mixing. However, at that time, these components that have various main components, have different deadlines (from origin) (plant-derived pearl barley to animal-derived placenta), and these components have no particular affinity or relationship between them. It was predicted that the two samples would interfere with each other and not even the effects of each single sample would be achieved, and it was confirmed that they did not interfere at all, contrary to the prediction. From this point of view, the present invention is far beyond the prediction of those skilled in the art.

(Test Example 2: Moisturizing effect improvement test)
NMF (Natural Moisturizing Factor) scattered in the stratum corneum in the skin has an important function in maintaining the moisture retention ability of the skin. When these are insufficient, the moisture of the stratum corneum is lost, and the stratum corneum overlaps. Causes rough skin and dryness. The main components of NMF are amino acids, lactic acid, urea, citrate, etc., all of which have the ability to hold moisture, and placenta extract and pearl barley are rich in these. Therefore, from this point of view, it can be expected that a solution in which placenta extract and pearl barley are mixed has a much higher moisturizing ability than a solution containing them alone.

(Test Example 3: Antioxidant activity improvement test)
When active oxygen is generated in the body due to ultraviolet irradiation, lipid peroxide is generated, which causes skin aging and wrinkles. There is a DPPH-VIS method using a stable radical DPPH (1,1-diphenyl-2-picrylhydrazyl) as a method for examining the effect (antioxidant activity) of preventing the formation of lipid peroxide and the like. DPPH is a stable purple radical itself, and in the presence of an antioxidant (hydrogen donor), it deprives hydrogen and changes to a non-radical form, and apparently purple gradually fades.
In order to examine the antioxidant activity of placenta extract and pearl barley, the following experiment was conducted using placenta solution (Nichirei Biosciences, Inc.) and the pearl barley extract powder described above.

(1) Experiment 200 μL of 200 μM DPPH solution and 100 μL of 0.2 M MES (2- (N-morpholino) ethanesulfonic acid) buffer were mixed. To the mixed solution, placenta solution, pearl barley extract aqueous solution and placenta extract powder dissolved in pearl barley extract powder were added at respective concentrations, and the absorbance at 520 nm after 20 minutes was measured, and the fading activity due to radical scavenging was regarded as Trolox equivalent. displayed. The sample was an average value obtained by analyzing three points.

(2) Results As is apparent from the results of FIG. 2 (DPPH radical scavenging activity (corresponding to Trolox)), 100 μL of the placenta solution showed radical scavenging activity equivalent to 22.7 μM Trolox. The pearl barley extract was 23.6 μM Trolox equivalent / mg. On the other hand, the sample in which pearl barley extract was dissolved in the placenta solution showed the strongest radical scavenging activity, and its value was 43.7 μM Trolox equivalent / mg (FIG. 2). By mixing the placenta solution and pearl barley extract, each showed stronger radical scavenging activity than the single sample, indicating the effectiveness of mixing. In addition, at that time, if placenta and pearl extract containing various complex components are used in combination, it is sufficiently predicted that the radical scavenging activity will be reduced or eliminated due to the mutual interference effect. It has been confirmed that mixing these two causes no such disadvantage, and it has been proved for the first time that the present invention has a remarkable effect far beyond prediction.

(Test Example 4: Transdermal absorbability improvement test)
When the liposome used in the present invention was measured with a particle size distribution analyzer, it was found that the diameter of the liposome was an average of 25 nm (FIG. 3). The intercellular distance of the skin is 250 nm, and the liposome used in the present invention is smaller than the intercellular distance of the skin and is sufficiently small to be absorbed to the deep part of the skin. From this, it can be expected that encapsulating the placenta extract in liposomes will increase the transdermal absorption of the placenta extract and dramatically improve the efficacy of the placenta. Furthermore, liposomes themselves are known to work as moisturizers when absorbed by the skin, and can be said to be excellent for use as a raw material for cosmetics.

(Example 1: Production of liposome)
Hydrogenated soybean lecithin 2-15%, 1,3-butylene glycol 2-7%, methyl paraoxybenzoate 0.1-0.4%, dihydrocholes-30 (trade name: Nikko Chemicals Co., Ltd.) 0.2- 0.9% (when blended), water-soluble placenta extract 5-50%, pearl powder (powdered pearl extract produced by the method described above) 0.5-30%, purified water residue (% above) (W / w)%), and placenta / barley encapsulated liposomes were produced.

  As an example, placenta / barley encapsulated liposomes were produced according to the formulation shown in Table 1 below.

First, hydrogenated lecithin, 1,3-butylene glycol and methyl paraoxybenzoate are weighed in the above blending ratio, and sufficiently stirred and dispersed at room temperature. Thereafter, the remaining raw materials are weighed and added, and further stirred.
When sufficiently stirred, the solution is heated to about 80 ° C. (77 to 83 ° C.) and stirred for 30 minutes with a homomixer (TK HOMMIXER MARK II, manufactured by Tokushu Kika Kogyo Co., Ltd.). It was dispersed with M-110-E / H (manufactured by Mizuho Kogyo Co., Ltd.), cooled, and sieved to obtain a placenta / barley-encapsulated liposome solution having a fine and homogeneous particle size (average particle size 25 nm).

(Example 2: Production of lotion)
Using the liposomes encapsulating the placenta and pearl barley described in Example 1 and having the formulation shown in Table 2, a skin lotion excellent in whitening action, moisturizing action and antioxidant action was obtained.

(Example 3: Production of serum)
Using the liposomes encapsulating the placenta and pearl barley described in Example 1 and having the composition shown in Table 3, a cosmetic liquid with excellent whitening action, moisturizing action and antioxidant action was obtained.

(Example 4: Production of gel cream)
Using the liposome encapsulating the placenta and pearl barley described in Example 1, a gel cream excellent in whitening action, moisturizing action and antioxidant action was obtained by the formulation shown in Table 4.

(Example 5: Production of emulsion)
By using the liposome encapsulating the placenta and pearl barley described in Example 1, the emulsion shown in Table 5 was used to obtain an emulsion excellent in whitening action, moisturizing action and antioxidant action. In the following, PCA soda refers to pyrrolidone carboxylate soda.

(Example 6: Sensory test)
A panel test of the cosmetics according to the present invention was conducted, and comparisons were made with various comparative cosmetics to confirm that the cosmetics according to the present invention are sensory.

(A) Test method The emulsion prepared by Example 5 (referred to as emulsion A), the placenta-encapsulated liposome solution in the emulsion A, the placenta-encapsulated liposome solution, the oat-encapsulated liposome solution, the placenta-encapsulated liposome solution, and the oat-encapsulated liposome solution Three types of emulsions replaced with an equal amount mixture were designated as emulsions B, C, and D, respectively, and a panel test was performed on these samples. As a control, an emulsion containing neither placenta nor pearl barley was used as an emulsion E for the test.

  The formulations of emulsions B, C, and D, which are comparative examples, are shown in Tables 6, 7, and 8 below, respectively. However, the “placenta-encapsulated liposome” and “barley-encapsulated liposome” described below contain 40 (w / w)% of placenta in the formulation of Example 1 and do not contain pearl barley or 1 (w / w) pearl barley. % Refers to liposomes produced without containing placenta.

  A sensory test was conducted on 10 women aged 25 to 58 (average age 38.1). In addition to the above-mentioned AD after washing the left arm, the subject was further coated with an appropriate amount of a control emulsion containing no liposomes twice a day in the morning and evening. Ten days later, the following four evaluation items were scored at 10 points for each person with 5 points as the emulsion E, and evaluated with a maximum of 100 points. The results are shown in Tables 9 and 10 below.

(1) The skin is moist (2) The skin is smooth (3) Feels that the penetration of the emulsion into the skin is good (4) The feeling of applying the emulsion lasts a long time

(C) Results The results of the panel test are shown in Tables 9 and 10 below.

(D) Results (summary)
The above results are summarized in Table 11 below.

  In the above table, 80 or more points are indicated as ☆, 70 to 79 points as ◎, 60 to 69 points as ○, and 50 to 59 points as △.

  As a result of the sensory test, the effect of the emulsion A was the highest in any of the items. Further, regarding the item “(4)“ A feeling of applying the emulsion lasts ””, the placenta-encapsulated liposome and the pearl-encapsulated liposome were mixed. In the case of only the emulsion (Emulsion D), it was confirmed that the effects were comparable to those of the emulsions (Emulsions B and C) used alone. Even if it compared with any of D, there existed a high effect. In particular, in the emulsions A and D having very similar blends, the evaluation of the emulsion A by one rank is higher than the emulsion D in all items, and the emulsion A according to the present invention is also functionally. It was proved that the cosmetics were excellent overall.

  Thus, according to the emulsion containing the placenta / barley-encapsulated liposomes according to the present invention as an active ingredient, after 10 days of use, the skin becomes more moist and smooth compared to the conventional product and the conventional product mixture. Feeling and the remarkable effect that the penetration of the emulsion into the skin was better.

(E) Improvement of feeling of use As is clear from the above results, according to the present invention, in addition to excellent medicinal effects, there are also sensory effects such as improvement in transdermal absorbability, feeling of use, comfort and feeling of tightness. Played. Since these effects are unique and very important for cosmetics, especially skin cosmetics, the present invention has a very remarkable effect, particularly that for skin cosmetics. be able to.

  Moreover, according to the present invention, compared with the case where the placenta-encapsulated liposome and the barley-encapsulated liposome are separately mixed (milky emulsion D), the case where the liposome containing the placenta and barley at the same time is encapsulated (milky milk A) ) Shows a much better effect. Although the detailed mechanism must be studied again in the future, the present invention is very specific and characteristic in that the effect varies greatly depending on the mode of encapsulation in the liposome while using the same raw material.

  In addition to the above, the present invention has the following features and effects.

(safety)
Since placenta extract and pearl barley are both natural ingredients, they are safe for the human body. In particular, pearl barley can be manufactured according to the manufacturing method as described above, and can be highly evaluated in terms of both quality and safety.

(Simple formulation)
Usually, in order to impart a whitening effect, a moisturizing effect, and an antioxidant activity effect to a cosmetic, it is necessary to blend a plurality of raw materials. However, according to the present invention, placenta extract and pearl barley cover these effects, so there is no need to add other raw materials. Therefore, cosmetics can be easily created.

  Moreover, according to the present invention, in addition to excellent medicinal effects (pharmacological effects), it is also characteristic in that it exhibits unique and important remarkable effects necessary for cosmetics that are “comfortable to use”, particularly skin cosmetics. is there.

  Therefore, the present invention is not only a cosmetic, a method for producing the same, but also a sensory aspect such that when a cosmetic (cosmetics, non-medical external skin preparation) is actually used, the feeling of use is improved and the makeup can be made comfortably. In addition, the remarkable effect is also played. Such sensory effects are unique to cosmetics and are as important to cosmetics as medicinal effects.

  In view of such importance, the present invention also provides a non-therapeutic cosmetic method (treatment method, cosmetic treatment method), and is exemplified.

(Example 1)
A non-therapeutic and comfortable cosmetic method characterized by applying a cosmetic containing the placenta / barley encapsulated liposome according to the present invention to the skin locally and / or systemically.
However, “comfortable” means at least one of the following (a) to (c).
(A) Improvement of percutaneous absorbability (feeling of penetration) (b) Improvement of feeling of use (c) Improvement of comfort

(Example 2)
Applying cosmetics containing the placenta / barley-encapsulated liposome according to the present invention to the skin locally and / or systemically to exert at least one effect of whitening, moisturizing, and antioxidant on the skin. A non-therapeutic comfortable cosmetic method according to Example 1 above, characterized by:

  The present invention is summarized as follows. That is, an object of the present invention is to remarkably improve the inherent effects (especially whitening effect, moisturizing effect, antioxidant effect) of placenta extract and newly develop a highly safe cosmetic.

And the said subject was solved with the cosmetics which enclosed the placenta pearl barley in the liposome. One example of its manufacture is as follows.
Dissolve lipid membrane components containing phospholipids and / or glycolipids in a solvent, add an aqueous phase containing water-soluble placenta kiss and pearl barley powder, stir, further heat, stir, disperse and disperse fine liposomes. Then, liposomes having an average particle size of 100 nm or less (for example, 20 to 25 nm) are separated and collected to obtain placenta / barley-encapsulated liposomes. This is used as a cosmetic as it is or as a cosmetic raw material to produce various cosmetics. The cosmetics according to the present invention are very characteristic in that in addition to the above-mentioned effects, sensory characteristics that are very important for cosmetics such as comfort are also used.

Claims (11)

A lipid membrane component composed of phospholipids and / or glycolipids and a solvent are mixed and stirred, an aqueous phase containing a water-soluble placenta extract and pearl barley powder is added to the obtained liquid, and the resulting liquid is stirred. After heating and stirring, the mixture is dispersed to form fine liposomes, and after cooling to room temperature or without cooling, liposomes having an average particle size of 100 nm or less are separated and collected using a sieve, and placenta pearl barley Obtaining encapsulated liposome liquid and producing cosmetics using the placenta / barley encapsulated liposome prepared in this way, at least one of improving transdermal absorbability, improving usability, and improving comfort The manufacturing method of the cosmetics which show | plays the effect of this. The method according to claim 1, wherein a placenta extract is used as the water-soluble placenta extract. Processed product obtained by adding water to the coarsely pulverized powder without husk or husks as pearl powder and adding an enzyme with protease activity and an enzyme with α-amylase activity. The method according to claim 1 or 2, characterized in that (pearl barley extract) or a powdered version thereof (barley extract powder) is used. The method according to any one of claims 1 to 3, wherein a liposome having an average particle diameter of 15 to 50 nm is used. The method according to any one of claims 1 to 4, wherein the cosmetic is a skin cosmetic. The method according to any one of claims 1 to 5, wherein the cosmetic has at least one of a whitening effect, a moisturizing effect and an antioxidant effect. A cosmetic comprising a liposome encapsulating placenta and pearl barley, produced by the method according to any one of claims 1 to 6. The makeup according to claim 7, which has at least one of a whitening effect, a moisturizing effect, and an antioxidant effect, and further has at least one effect of improving percutaneous absorption, improving the feeling of use, and improving the feeling of use. Fee. The cosmetic is at least one of lotion, cosmetic liquid, milky lotion, cream, pack, mask, bathing agent, cleaning agent, dispersion liquid, ointment, liquid agent, aerosol, and patch. Or the cosmetic according to 8. A non-therapeutic comfortable cosmetic method characterized by applying the cosmetic according to any one of claims 6 to 9 to the skin locally and / or systemically.
However, “comfortable” means at least one of the following (a) to (c).
(A) Improvement in percutaneous absorption (b) Improvement in usability (c) Improvement in comfort Applying the cosmetic according to any one of claims 6 to 9 to the skin locally and / or systemically to exert at least one effect of whitening, moisturizing, and antioxidant on the skin, The non-therapeutic comfortable cosmetic method according to claim 10.

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