JP2013237644A - Cell activating carbonated water - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a carbonated water having a cell activating function.SOLUTION: A composition comprising pyrroloquinoline quinone or its salt and a source for generation of carbon dioxide or optionally a composition comprising pyrroloquinoline quinone or its salt, a source for generation of carbon dioxide and a dispersion medium are provided.

Description

  The present invention relates to a composition comprising pyrroloquinoline quinone, carbon dioxide and water.

Carbonated water is a solution in which carbon dioxide is dissolved in water, and is often used as a beverage, but also used for bathing (Patent Documents 1 and 2) and a beauty method (Patent Document 3). Widely used, such as fine carbonated water with low carbon dioxide concentration, carbonated drinking water with high carbon dioxide concentration under high pressure, artificial carbonated springs in which carbon dioxide is dissolved in warm water, and carbon dioxide solution used for industrial use Yes.

The vasodilation effect when bathing in warm water containing carbon dioxide such as carbonated springs and the warm bath effect such as being hard to cool down are generally well known and have been used in bathhouses using hot springs for a long time. It is thought that the warming action of carbonated spring is basically due to the improvement of the body environment due to the peripheral vasodilatory action of the contained carbon dioxide. In addition, the percutaneous approach of carbon dioxide increases and dilates the capillary bed, improving skin circulation. For this reason, it is said that it is effective in the treatment of degenerative lesions and peripheral circulation disorders.

On the other hand, pyrroloquinoline quinone (hereinafter sometimes referred to as PQQ) has been proposed as a new vitamin (Non-patent Document 1), and has attracted attention as a useful substance for health supplements, cosmetics, and the like. Collecting. Furthermore, PQQ is present not only in bacteria but also in eukaryotic molds and yeasts, and plays an important role as a coenzyme. As for PQQ, there are many cell growth promoting effects, anti-cataract effects, liver disease desired treatment effects, damage healing effects, anti-allergic effects, reverse transcriptase inhibitory effects and glyoxalase I inhibitory-anticancer effects by the recent years. Physiological activity has been elucidated.

Japanese Patent Laid-Open No. 2005-137452 JP 2008-137957 A JP 2003-073253 A

nature, vol.422, 24April, 3003, p832

A solution in which carbon dioxide is dissolved is used in a wide range of situations as described above, and further, it is desired to have a new function. An object of the present invention is to provide a carbonic acid solution having a function of activating cells.

According to the present invention, the following inventions are provided.
[1] A composition comprising pyrroloquinoline quinone or a salt thereof and a carbon dioxide generating source.
[2] The composition according to [1], further comprising a dispersion medium.
[3] The composition according to [1], wherein the carbon dioxide generation source is carbonate and further contains an acid.
[4] The composition according to [2] or [3], wherein the salt of pyrroloquinoline quinone is an alkali metal salt.
[5] The composition according to [2] or [4], wherein the dispersion medium contains water.
[6] The composition according to [3] or [4], wherein pyrroloquinoline quinone or a salt thereof is 0.0001 to 10% by weight, carbonate is 5 to 80% by weight, and acid is 5 to 80% by weight.
[7] The composition according to any one of [2], [4] or [5], wherein the concentration of carbon dioxide in the dispersion medium is 0.1 to 1.5% by weight.
[8] The composition according to any one of [2], [4], [5] or [6], wherein the concentration of pyrroloquinoline quinone or a salt thereof in the dispersion medium is 0.001-1% by weight. object.
[9] The composition according to any one of [2], [4], [5], [6] or [7], which is in a gel form.
[10] The composition according to any of [3], [4] or [6], which is in the form of a tablet.
[11] The composition according to any of [3], [4] or [6], which is in the form of a powder.
[12] The composition according to any one of [1] to [11], which is a bath agent.

The present invention makes it possible to provide a carbonic acid solution having a cell activation function. Further, the composition liquid can easily improve the solubility at room temperature.

The present invention is a composition comprising pyrroloquinoline quinones and a carbon dioxide source. A pyrroloquinoline quinone refers to pyrroloquinoline quinone, a salt thereof, or a hydrate thereof. The pyrroloquinoline quinone is a compound having a structural formula represented by the following formula (1).
Examples of the salt of pyrroloquinoline quinone used in the present invention include alkali metal salts, alkaline earth metal salts, and ammonium salts of pyrroloquinoline quinone, with alkali metal salts being preferred. Examples of the alkali metal salt include salts of sodium, potassium, lithium, calcium, magnesium, cesium, rubidium and the like. Preferably, it is a sodium salt. The salt of pyrroloquinoline quinone may be used alone or in combination.

The number of substitutions in the alkali metal salt of pyrroloquinoline quinone is 1 to 3, and any of a monoalkali metal salt, a dialkali metal salt, and a trialkali metal salt may be used, but a dialkali metal salt is preferred. More preferably, it is a disodium salt.
As the salt of pyrroloquinoline quinone used in the present invention, a commercially available salt can be obtained, and it can be produced by a known method.

Examples of the carbon dioxide generation source include carbon dioxide, carbonate, bicarbonate and the like. When carbon dioxide is used as the carbon dioxide generation source, it is preferable to further include a dispersion medium. As a specific dispersion medium, one or more kinds of water and various organic solvents can be used, and at least water is preferably used. As water to be used, distilled water, ion exchange water, soft water, hard water and the like can be used without limitation. Although there is no restriction | limiting in particular also about an organic solvent, For example, ethanol, a propanol, a butanol, glycerol, propylene glycol is mentioned.

When carbonate or bicarbonate is used as the carbon dioxide generation source, it is preferable to further use an acid. Specific examples of the carbonate include sodium bicarbonate, sodium carbonate, potassium bicarbonate, potassium carbonate, carbonate such as calcium carbonate, and bicarbonate.
As the acid, hydrochloric acid, sulfuric acid, nitric acid, citric acid, acetic acid, edetic acid, lactic acid, phosphoric acid, succinic acid, malonic acid or an acid salt can be used. Examples of the salt exhibiting acidity include sodium sulfate, potassium sulfate, sodium phosphate, and potassium phosphate. The pyrroloquinoline quinones are preferably mixed at 0.0001 to 10% by weight, carbonate at 5 to 80% by weight, and acid at 5 to 80% by weight.

As a method for producing the composition of the present invention, a method of mixing pyrroloquinoline quinones and a carbon dioxide source,
A method of dissolving pyrroloquinoline quinones and a carbon dioxide source in a dispersion medium,
A method of adding a carbon dioxide generating source after mixing pyrroloquinoline quinones and a dispersion medium,
Examples thereof include a method of adding pyrroloquinoline quinones to a carbonic acid solution in which a carbon dioxide generating source is previously dissolved.

The dispersion medium is preferably added and prepared so that the concentration of pyrroloquinoline quinones is 0.001 to 1% by weight and the concentration of carbon dioxide is 0.1 to 1.5% by weight. To increase the concentration of carbon dioxide beyond this, special measures such as pressurization and the use of special membranes are required. If the concentration is lower than this, the refreshing feeling and the foaming when drinking are not recognized.

  The mixed composition liquid may be stirred. Stirring can be carried out by subjecting to magnetic stirring, mechanical stirring, manual stirring, shaking stirring, etc., preferably mechanical stirring, manual stirring, and shaking stirring.

When carbonate is used as a carbon dioxide generation source, it is necessary to use an acid. The pH of the final composition liquid is preferably 1 to 9, more preferably 2 to 5. If the pH is too high, carbon dioxide forms bicarbonate ions and carbonate ions, and foaming properties are easily lost, which is not preferable. On the other hand, if the pH is too low, pyrroloquinoline quinone itself precipitates, which is not preferable.

The addition of the carbon dioxide source is preferably performed at -20 to 100 ° C. More preferably, it is −4 to 60 ° C. When the temperature is high, carbon dioxide tends to escape. If it is too low, the composition cannot be maintained by freezing.

  The composition of the present invention can be used for foods, cosmetics, bath agents, pharmaceuticals and the like. Other components necessary for it can be added as needed. Flavor, acidulant, salt, umami ingredient, fruit juice, fermented food, lipid, moisturizer, whitening agent, sweetener, herb extract, tea, coffee, emulsifier, glycerin, preservative, antibacterial agent, steroid, methyl salicylate, vitamin, What is necessary is just to add indomethacin etc. as needed.

  When used in food, pharmaceutical and cosmetic applications, it is natural to pay attention to hygiene, and it is desirable to manufacture in an aseptic environment such as a clean room.

When mixing sweeteners, monosaccharides, disaccharides, oligosaccharides, and artificial sweeteners can be mixed. Examples of artificial sweeteners include fructose, glucose, galactose, sorbitol, xylitol, erythritol, trehalose, palatinit, aspartame, acesulfame K, sucralose, licorice extract, lacanca, sucrose, starch syrup, honey and the like. Moreover, coenzyme Q10 used as an antioxidant, vitamins, polyphenol, phospholipid, etc. can also be added.

The composition of the present invention can be provided in the form of a powder, tablet or gel. When providing in the form of a gel state, it is also possible to mix a normal gelling agent. Examples include gelatin, agar, carrageenan, collagen, fucoidan, hyaluronic acid, konjac, glucomannan, pectin, locust bean gum, xanthan gum, gellan gum, starch, egg white and the like.

The present invention will be described more specifically with reference to examples and comparative examples, but the present invention is not limited to these examples.
In this example and comparative example, UV measurement was performed using a HITACHI U-2000 Spectrophotometer. As a raw material, pyrroloquinoline quinone disodium trihydrate (Mitsubishi Gas Chemical Co., Ltd., powder) was used.

Example 1-3, Comparative Example 1-8
Cell activation test of carbonated water containing pyrroloquinoline quinone Dissolve pyrroloquinoline quinone disodium trihydrate at various concentrations in carbonated water to prepare test solutions so that the concentrations shown in Table 1 were obtained. As a comparative example, pyrroloquinoline quinone disodium trihydrate or carbonated water dissolved in water was used. This was performed using Chinese hamster ovary cells (CHO cells). The medium used was α-MEM medium (GIBCO) supplemented with 10% fetal bovine serum (FBS) and a small amount of penicillin, streptomycin, and kanamycin. Culturing was performed at 37 ° C. under saturated water vapor with 5% by weight of carbon dioxide added. The cultured CHO cells were detached from the petri dish by trypsin treatment. The detached cells were removed by centrifugation and a medium was added. This was put into a hemocytometer and the number of cells was counted.
Using a 96-well plate, 5000 cells were placed in the well and cultured overnight at 100 μL / well of medium.
After overnight culture, the test solution was replaced with a solution diluted 64-fold with the medium. One day later, the medium was removed, washed twice with the medium, and the number of cells was counted by measuring absorbance at 450 nm with a plate reader using WST (doujinn Cell Counting kit 8). The absorbance at this time is proportional to the number of cells. The number of cells when nothing was added was calculated as 100. The results are shown in Table 1.
The carbonated water containing PQQ of Example 1 has a proliferation effect compared to the carbonated water and PQQ aqueous solution alone shown in Comparative Examples 2 and 3. Synergistically activates cells. Example 3 is more effective than Comparative Examples 5 and 6, and Example 4 is more effective than Comparative Examples 7 and 8.

Example 4 Dissolution of PQQ disodium in carbonated water 0.1 g of PQQ disodium was added to 1 ml of carbonated water of 0.9% by weight carbon dioxide, heated at 40 ° C. for 30 minutes, returned to room temperature, and centrifuged. I got a supernatant. As a result of diluting to 1/1000 with water and measuring the concentration at UV 250 nm, the dissolution amount was 8.1 g / L pH 3.6.

Comparative Example 9
The same operation as in Example 4 was performed by changing from carbonated water to water. The dissolution amount was 3.0 g / L pH 3.5.
By comparing the results of Example 4, the carbonated water was dissolved 2.7 times compared to the solubility in water, and there was an effect of promoting dissolution by carbon dioxide.

Example 5 Saccharose 10 g and pyrroloquinoline quinone disodium trihydrate 15 mg were added to carbonated water carbon dioxide 0.9 wt% carbonated water to which sugar was added. A uniform and sweet solution could be produced.

Example 6 Carbonation generating solid sodium hydrogen carbonate 1.2 g, sodium carbonate 0.8 g, pyrroloquinoline quinone disodium trihydrate 0.006 g, and succinic acid 1.994 g were mixed. 0.2 g of this mixture was taken and added to 1 L of hot water (40 ° C.). It became almost colorless and transparent foaming hot water, and a solution containing PQQ that generates carbon dioxide could be made. This solution is suitable for bathing and the like.

Example 7 Carbonation generation solid sodium hydrogencarbonate 1.2g, sodium carbonate 0.8g, pyrroloquinoline quinone disodium trihydrate 0.006g, and citric acid 1.994g were mixed. 0.2 g of this mixture was taken and added to 1 L of hot water (40 ° C.). It became almost colorless and transparent foaming hot water.

Comparative Example 10
Sodium hydrogen carbonate 1.2 g, sodium carbonate 0.8 g, and pyrroloquinoline quinone disodium trihydrate 0.006 g were mixed. 0.2 g of this mixture was taken and added to 1 L of hot water (40 ° C.). It was a solution in which almost colorless carbon dioxide was not generated. It has been found that this composition does not generate carbon dioxide and the addition of acid is necessary for foaming.

The present invention can be used for foods and drinks, bathing agents, cosmetics, pharmaceuticals and the like.

Claims (12)

A composition comprising pyrroloquinoline quinone or a salt thereof and a carbon dioxide source. The composition of claim 1 further comprising a dispersion medium. The composition of claim 1, wherein the carbon dioxide generating source is carbonate and further comprises an acid. The composition according to claim 2 or 3, wherein the salt of pyrroloquinoline quinone is an alkali metal salt. The composition according to claim 2 or 4, wherein the dispersion medium contains water. The composition according to claim 3 or 4, wherein pyrroloquinoline quinone or a salt thereof is 0.0001 to 10% by weight, carbonate is 5 to 80% by weight, and acid is 5 to 80% by weight. 6. The composition according to claim 2, wherein the concentration of carbon dioxide in the dispersion medium is 0.1 to 1.5% by weight. The composition according to any one of claims 2, 4, 5 and 6, wherein the concentration of pyrroloquinoline quinone or a salt thereof in the dispersion medium is 0.001-1% by weight. The composition according to any one of claims 2, 4, 5, 6, or 7, which is in a gel form. A composition according to any of claims 3, 4 or 6, which is in the form of a tablet. A composition according to any of claims 3, 4 or 6, which is in the form of a powder. The composition according to any one of claims 1 to 11, which is a bath agent.