KR20190021668A - Composition having Antimicrobial Effects on Acnes Bacteria Using Solid Phase Lactobacillus-fermentation Product of Prickly Pear Fruits - Google Patents

Abstract

The present invention discloses a composition having antimicrobial effects on acne bacteria using a solid phase Lactobacillus fermented product of Opuntia ficus-indica fruits. The solid phase fermented product is obtained by preparing solid phase pills using fruit powder of Opuntia ficus-indica fruits and inoculating Lactobacillus plantarum into the pills.

Description

[0001] The present invention relates to an antimicrobial composition for acne bacterium using a solid fermented product of lactic acid bacteria,

The present invention relates to a method for producing lactic acid bacteria of Opuntia ficus-indica ( Opuntia ficus-indica ) This invention relates to an antimicrobial composition against Propionibacterium acnes which is an acne bacterium using a fermented product.

It is a tropical naturalized plant belonging to the cactus ( Opuntia ) of Cactaceae. In Korea, it grows in the south coast region such as Jeju Island and Sinan County. .

The perennial plant is resistant to drought and its fruit is used for food. There are known anti-ulcer effects, diuretic effect, prevention of hypertension, lowering of cholesterol, hypoglycemic effect, antidiabetic effect, wound healing effect, anti-inflammatory effect and antioxidant effect (Molecules 19: 14879-14901, 2014; J. Food Preserv. 16: 953-958, 2009; J. J. Food Preserv. 6: 345-349, 1999. J. Fd Hyg. Safety 27: 401-405, 2012. J Ethnopharmacol 76: 1-9, 2001).

Acne is a polymorphic skin disease that occurs in most people in all ages and occurs in the form of facial, chest and shoulder patches, pustules, papules and nodules.

It mainly affects adolescents with maturity. It has been reported that about 80% of cases develop from 11 to 30 years of age and about 6% of cases occur after 30 years of age. Recently, however, the incidence of adult acne due to stress has been increasing. The disease can be uncomfortable to the patient and cause aesthetic damage due to scarring and skin pigmentation.

 The exact cause of acne has not been established, and because of its various physiological and environmental aspects, it is difficult to properly treat acne of various types.

Usually caused by excessive secretion of sebum, a complex of factors, including hormonal imbalance, genetic predisposition, abnormal hair follicle keratinocytes and at least within P. acnes proliferation, and inflammation of the sebaceous glands, and (Acne:... Inflammation Clin Dermatol 22: 380- As a basic method for the treatment of acne, it can be used to correct trait changes due to follicular keratinization, reduce the activity of sebaceous glands, induce acne Antimicrobial action against major strains, and skin anti-inflammatory action have been applied.

Normal skin contains aerobic bacteria such as Staphylococci, Micrococci and anaerobic bacteria belonging to Propionibacterium, and the activity of these strains may cause acne. In particular, P. acnes , a lipophilic anaerobic strain, has been identified as a major cause of acne (Postgrad. Med. J. 75: 328, 1999).

P. acnes , a major pathogen of acne, is a lipophilic microorganism that grows in the skin follicle as an anaerobic mantle in the skin. P. acnes hydrolyzes the triglycerides of sebum produced by androgen and other factors, and produces free fatty acids through the secretion of lipases and chemotactic factors, resulting in inflammatory responses . As the inflammatory reaction progresses, the hair follicles are destroyed and finally the inflammation reaction progresses to the dermis. Dermatol, 14 (6): 480-488, 1997), which is a systemic treatment for the development of acne caused by bacterial infections such as P. acnes (Pediatr.

Antibiotics used to treat acne include tetracycline, clindamycin, and the like. However, long-term use of antibiotics may result in tolerance and reduced efficacy (Kor, J. Dermatol. 33: 437-444, 1995).

Recently, many studies have been conducted on the antimicrobial activity of natural products such as herbal medicines to prevent or cure acne (Kor. J. Microbiol. Biotechnol. 37 (1): 80-84, 2009).

The present invention discloses an antimicrobial activity against Propionibacterium acnes which is an acne bacterium of lactic acid bacteria solid phase fermented product of Baekjunseok .

An object of the present invention is to provide an antimicrobial composition against Propionibacterium acnes , an acne bacterium using lactic acid fermented product of Opuntia ficus-indica ( Opuntia ficus-indica ) fruit.

 Other and further objects of the present invention will be described below.

As shown in the following Examples and Experimental Examples, pancakes of solid phase using powdery white perennials were prepared and Lactobacillus plantarum KCCM 80077 ( Lactobacillus plantarum KCCM 80077), which is a lactic acid bacterium, ) Was inoculated to produce a fermented product of fruit perennial plant, which was completed by confirming that the extract had antimicrobial activity against Propionibacterium acnes , an acne bacterium. The extract of Lactobacillus plantarum KCCM 80077 and the extract of Lactobacillus plantarum KCCM 80077 showed no antimicrobial activity against Propionibacterium acnes , an acne bacterium, and it was also shown in the following experimental examples However, the extract of Lactobacillus plantarum KCCM 80077 culture itself did not exhibit antibacterial activity.

The antimicrobial composition for Propionibacterium acnes , an acne bacterium of the present invention, is provided on the basis of the results of these experiments. The antimicrobial composition of the present invention is prepared by adding purified water (solid Solid phase product obtained by inoculating Lactobacillus plantarum, which is a lactic acid bacterium, as an active ingredient.

As used herein, the term " active ingredient " refers to an ingredient that alone exhibits the desired activity or that can exhibit activity with a carrier that is itself inactive.

In the present specification, the term " solid-phase fermented product extract " refers to an extract of a solid object itself or its powder as a target to be extracted with water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol), methylene chloride, ethylene, acetone, , An extract obtained by leaching using chloroform, ethyl acetate, butyl acetate, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, Refers to an extract obtained by using a supercritical extraction solvent such as carbon dioxide, pentane, or a fraction obtained by fractionating the extract. The extraction method is a method of extracting the supernatant from a supernatant, such as cold water, reflux, warming, Any method such as supercritical extraction can be applied. In the case of the fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and leaving with a solvent having a different polarity, the crude extract is adsorbed on a column packed with silica gel, and then a hydrophobic solvent, a hydrophilic solvent, Quot; means fractions obtained as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably means an extract obtained by using water, a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.) or a mixed solvent thereof as an extraction solvent.

In the present invention, the shape of the solid object is not particularly limited. Pills, granules, and the like.

In the present invention, the solid matter may be obtained by adding purified water to the powder of lactic acid bacteria, kneading the same, and shaping the powder into a predetermined shape. In this case, the purified water may be added in an amount of 10 to 30 parts by weight based on 100 parts by weight of the fruit powder. If it is added in an amount of 10 parts by weight or less, sufficient dough may not be produced for the production of the solid, and if it exceeds 30 parts by weight, it may be difficult to produce the solid in the form of a large amount of water. After shaping into a predetermined shape, it is dried in an arbitrary manner such as natural drying (shade) or hot air drying, so that a part of the water is removed (the water content of the solid is less than 10% (w / w) It is possible.

In the present invention, the lactic acid bacterium, Lactobacillus plantarum, is preferably Lactobacillus plantarum KCCM 80077 used in the following examples.

In the present invention, the lactic acid bacterium is inoculated with a lactic acid bacterium inactivated by a lactic acid bacterium activated with a bacterium extract (including hot water) before inoculation, specifically, a carbon source such as a bacterium, Or cultured in the same manner as described above. Although the present inventors have not shown in the following Experimental Example, it is believed that fermentation will proceed to the deep portion of the pancake when lactic acid bacteria activated with Baekseoncho extract are used. The lactic acid bacillus which is activated by the extract of water of Baekjangju (including hot water) can be obtained by preparing a medium containing the Baekjangjang water extract and the carbon source and inoculating the lactic acid bacterium thereto, wherein the carbon source is any carbon source, The present invention relates to a pharmaceutical composition containing the compound of formula (I) or a pharmaceutically acceptable salt thereof, such as oligosaccharide, lactose, glucose, fructose, sugar, molasses, dextrose, starch, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, (W / v), and the medium may further contain a nitrogen source or a trace element in the range of 0.1 to 3% (w / v) Examples of the nitrogen source include ammonia, ammonium salts, urea, amino acids, soybean meal, soybean protein, yeast extract, and juice. Examples of the trace elements include calcium, magnesium, sodium, , Molybdenum, potassium, manganese, zinc, copper, iron, phosphorus, sulfur and so on. The cultivation can be carried out at 25 캜 to 40 캜, preferably 35 to 37 캜, for about 12 hours to 2 days.

In the present invention, the culture after inoculation may be carried out at a temperature of 15 ° C to 45 ° C, particularly at a temperature of 30 ° C to 40 ° C. If the incubation temperature is lower than the above range, the fermentation rate may be slowed and unwanted fermentation products may be produced. Even if the incubation temperature is higher than the above range, the fermentation rate may be similarly slowed down due to the death of the fermentation microorganisms, . The incubation time can be from 1 day to 7 days.

The antimicrobial composition of the present invention can be used in an amount of from 0.0001 to 20% by weight, preferably from 0.0001 to 20% by weight, based on the total weight of the composition, depending on the form of the antimicrobial composition of the present invention, Can be contained in the range of 0.0001 wt% to 15 wt%, more preferably 0.0001 wt% to 10 wt%.

The antimicrobial composition of the present invention may be prepared by including a dispersant and a carrier in addition to the effective ingredient.

Such dispersants include water, alcohols such as methyl alcohol, ethyl alcohol, ethylene glycol, propylene glycol, diethylene glycol, glycerin, etc., ketones such as acetone and methyl ethyl ketone, ethers such as dioxane, Aliphatic hydrocarbons such as hexane and kerosene; aromatic hydrocarbons such as benzene, toluene, xylene, naphthalene and methylnaphthalene; halogenated hydrocarbons such as chloroform, Carbon tetrachloride and the like), acid amides (e.g., dimethylformamide), esters (e.g., methyl acetate ester, ethyl acetate ester, butyl acetate ester, fatty acid glycerin ester and the like), nitrol (e.g., acetonitrile) Surfactants (higher alcohol sulfate esters, alkyl sulfonic acids, alkyl allyl sulfonic acids, quaternary ammonium salts, oxyalkylamines, fatty acid esters A polyalkylene oxide compound, and an anhydrous sorbitol compound). The dispersant may be incorporated into the antimicrobial composition of the present invention alone or as a mixture of two or more thereof.

Examples of the carrier include clay (e.g., kaolin, bentonite, acid clay, etc.), talc (such as talc powder and tin powder), silica (such as diatomaceous earth, silicic anhydride, and mica powder), alumina, sulfur powder, May be exemplified, and these carriers may be prepared by being included in the antimicrobial composition of the present invention alone or as a mixture of two or more kinds.

In a specific embodiment, the composition of the present invention can be identified as a pharmaceutical composition. When the composition of the present invention is identified as a pharmaceutical composition, its pharmacological effect or its use can be understood as prevention or treatment of acne due to antimicrobial activity against acne bacteria.

The pharmaceutical composition of the present invention may be prepared into oral formulations or parenteral formulations according to the route of administration by conventional methods known in the art, including pharmaceutically acceptable carriers in addition to the active ingredient. Where the route of administration may be any suitable route including local routes, oral routes, intravenous routes, intramuscular routes, and direct absorption through mucosal tissues, and combinations of two or more routes may be used. An example of a combination of two or more routes is a combination of two or more formulations of the drug according to the route of administration, for example, one drug is administered intravenously and another drug is administered via a local route.

Pharmaceutically acceptable carriers are well known in the art depending on the route of administration and formulation, and specific reference may be made to the pharmacopoeia of each country, including the " Korean Pharmacopoeia ".

When the pharmaceutical composition of the present invention is prepared into an oral formulation, it may be formulated into powder, granules, tablets, pills, sugar tablets, capsules, solutions, gels, syrups, suspensions, wafers And the like. Examples of suitable carriers include starches such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol and xylitol, corn starch, potato starch and wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Hydroxypropylmethylcellulose and the like; polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol Serol, and the like. In case of formulation, suitable binders, lubricants, disintegrants, coloring agents, diluents and the like may be included as needed. Examples of suitable binders include starch, magnesium aluminum silicate, starch pellets, gelatin, methyl cellulose, sodium carboxymethyl cellulose, polyvinyl pyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, wax and the like. Examples of the disintegrating agent include starch, methylcellulose, magnesium stearate, magnesium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, Agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt, and the like. Examples of the diluent include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine and the like.

When the pharmaceutical composition of the present invention is prepared into a parenteral dosage form, it may be formulated in the form of an injection, transdermal drug delivery, nasal aspirate and suppository together with a suitable carrier according to methods known in the art. As the carrier suitable for injection preparation, aqueous isotonic solutions or suspensions may be used. Specifically, PBS (phosphate buffered saline) containing triethanolamine, sterile water for injection, and isotonic solution such as 5% dextrose may be used . When formulated with a transdermal preparation, it can be formulated in the form of ointments, creams, lotions, gels, external liquids, pastes, liniments, and air-lozenges. Nasal inhalers may be formulated in the form of aerosol sprays using suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc., and when formulated as a suppository, witepsol, tween 61, polyethylene glycols, cacao butter, laurin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, and sorbitan fatty acid esters.

The formulation of pharmaceutical compositions is well known in the art and can be found, for example, in Remington ' s Pharmaceutical Sciences (19th ed., 1995). This document is considered part of this specification.

The preferred dosage of the pharmaceutical composition of the present invention is 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g / day, depending on the patient's condition, body weight, sex, age, / kg < / RTI > The administration can be carried out once or several times a day. Such dosages should in no way be construed as limiting the scope of the invention.

In another specific embodiment, the composition of the present invention can be identified as a cosmetic composition.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition may be classified into any product category according to the use of the cosmetic composition. Specifically, the cosmetic composition may contain functional cosmetic products having applications such as improvement of skin troubles and improvement of atopic dermatitis, General cosmetics, and the like. Specific examples of the product form include a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax Foundation, spray, and the like. In a specific product form, it may be a form of flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

The cosmetic composition of the present invention may contain, in addition to its active ingredient, conventional additives such as stabilizers, solubilizing agents, surfactants, vitamins, colorants and antioxidants, and carriers commonly used in cosmetic compositions.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component. Specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

When the formulation of the present invention is a suspension, a carrier, such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention can be produced according to a method for producing a cosmetic composition which is usually carried out in the art, except that it contains the effective ingredient showing skin wrinkle improving activity.

As described above, according to the present invention, it is possible to provide a fermented fermented product of lactic acid bacteria having an increased antioxidative activity and an increased likeness such as taste, flavor, texture and the like.

1 is a plate culture photograph.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Production of lactic acid fermented product

(1) For the production of lactic acid fermentation product of 100 years old, 15 parts by weight of purified water of 100 parts by weight per 100 parts by weight of the perennial plant was added to the finely ground perennial plant powder, kneaded and kneaded using a ventilator to form a 3-4 mm diameter ring, And then dried at 65 ~ 70 ℃ for 6 ~ 12 hours to partially remove the water (10% (w / w) water content of the ring). Next, the lactic acid bacteria diluted solution was sprayed using a sprayer sterilized in a non-fermentation ring, and the mixture was sprayed in a sealed container at a temperature of 30 to 37 ° C for 1 to 5 days (Production Example 1 to 5, , The fermentation was carried out at a temperature of 65 to 70 ° C. for 6 to 12 hours to prepare a fermented product of lactic acid bacteria in the form of a ring.

(2) The diluted lactic acid bacterium was prepared by adding 1 L of purified water corresponding to 20 times (w / w) to the dried powder of Baicin Seaweed, subjecting it to hot water extraction at 100 ° C for 1 hour, filtering the resulting extract with a 0.45 μm microfilter, Were added and mixed with 4% (w / v) molasses, 1.2% (w / v) yeast extract and 0.5% (w / v) calcium carbonate and sterilized at 121 ° C for 15 minutes to prepare a medium. (V / v) of the Lactobacillus plantarum KCCM 80077 culture prepared in a DeMan, Rogosa, and Sharpe broth medium and cultured at a temperature of 30 ° C to 37 ° C for 20 hours. After culturing, For 15 minutes to remove the supernatant, and physiological saline was added and diluted as much as the supernatant.

<Comparative Example> Production of non-fermented beans and fermented broth of lactic acid bacteria

&Lt; Comparative Example 1 & Production of non-fermented perennials

The production of non-fermented perennials was carried out in the same manner as in the above example. Specifically, 15 parts by weight of purified water of 100 parts by weight per 100 parts by weight of 100 parts by weight of the perennial plant was added to the finely pulverized perennial plant powder, and the plant was kneaded using a ventilating machine to form a 3 to 4 mm diameter circle, And dried at ~ 70 ° C for about 6 to 12 hours to partially remove water.

&Lt; Comparative Example 2 & Preparation of fermentation broth of lactic acid bacteria

The fermentation of lactic acid bacteria was carried out by adding 1 L of purified water of 20 times (w / w) to the dried powder of Baekseongchocho, hot water extraction at 100 ℃ for 1 hour, filtering the extract with 0.45 m microfilter, The medium was prepared by adding 4% (w / v) molasses, 1.2% (w / v) yeast extract and 0.5% (w / v) calcium carbonate and sterilization at 121 ° C for 15 minutes. Cells of Lacobacillus plantarum KCCM 80077 prepared in a MRS (DeMan, Rogosa, and Sharpe) plate medium were inoculated in the prepared medium and cultured at a temperature of 30 to 37 ° C for 20 hours. The culture broth was sterilized again at 121 ° C for 15 minutes and then lyophilized to obtain a powder.

< Experimental Example > Antibacterial activity against acne bacteria - Measurement of inhibition of growth

In order to prepare a sample for measuring the antimicrobial activity against acne bacteria, 20 μl (w / v) was added to each of the non-fermented perennials of Comparative Example 1 and each of the fermented fermented lactic acid bacteria of each of the above- (Ethanol) was added for 24 hours and then filtered using a 0.45 m microfilter. The filtrate was concentrated under reduced pressure and lyophilized to prepare a powdery extract. For the culture of the lactic acid bacteria of the comparative example 2, 1 L of 70% alcohol (ethanol) corresponding to 20 times (w / v) was added to the lyophilized powder at the room temperature for 24 hours, The process of filtration using a microfilter was repeated three times, and the filtrate was concentrated under reduced pressure and lyophilized to prepare a powdery extract.

100 μl of the 100 mg / mL sample solution and 100 μl of sterilized purified water were mixed at a ratio of 1: 1 (v / v) to prepare a sample solution having a concentration of 100 mg / mL by adding 50% ethanol to each of the obtained powdery extracts The mixture was finally mixed with a 25% EtOH solution so as to have a concentration of 50 mg / mL and used as a sample for antimicrobial activity measurement.

The strains used for antimicrobial activity measurement were purchased from KCTC (Korea Collection for Type Cultures) as propionibacterium acnes . The culture medium of P. acnes was activated by using RCM (Reinforced clostridial medium). After incubation of the bacteria in the culture medium, Anaerobic jar was sealed with AnaeroGen (oxoid) and anaerobically cultured at 37 ° C for 48 hours Respectively.

The clear zone measurement method was modified by the CLSI (Clinical and Laboratory Standards Institute) standard method. Specifically, strains isolated from platelets were platinum, suspended in 10 mL of RCM liquid medium, and then stock culture was performed using spectrophotometer at an absorbance ranging from 0.08-0.10 at 600 nm. Stock cultures were diluted 10-fold to prepare working cultures, and 100 μl of the culture was spread on a flat plate. After sterilized filter paper discs (8 mm, Tokyo, Japan) were placed on the surface of the RCM solid plate culture medium, 50 μl of the 50 mg / ml sample solution was absorbed and inoculated. After the incubation, the clear zone (mm) diameter around the disc was measured four times per disc.

In the control, clindamycin (control 1) and tetracycline (control 2), known as antibiotics for acne, were diluted in sterile purified water at a concentration of 10%, and 25% EtOH solution (control 3) Were prepared and used. The results are shown in Table 1 below and the mean deviation of the values except for the diameter of filter paper disc (8 mm) was obtained from the results of three repeated experiments for each concentration, and representative plate culture photographs of each group were shown in FIG. 1 Respectively.

Inhibition of growth sample Diameter of clear zone (mm) Control 1 26.0 ± 2.4 Control 2 14.1 ± 4.1 Control 3 N.D. Comparative Example 1 N.D. Comparative Example 2 N.D. Production Example 1 18.8 ± 1.3 Production Example 2 17.2 ± 0.1 Production Example 3 18.4 ± 1.0 Production Example 4 18.3 ± 0.4 Production Example 5 15.8 ± 1.3

As can be seen from the results of [Table 1] and [Figure 1], the non-fermented perennial plant extract of Comparative Example 1 and the perennial plant extract of Fermentation Lactic acid bacteria of Comparative Example 2 showed no antimicrobial activity against the acne strain, P. acnes . 25% EtOH (control 3) showed no antimicrobial activity. The antimicrobial activity of 10% clindamycin (control 1) was higher than that of 10% tetracycline (control 2).

Fermented product of Comparative Example 1 showed no antimicrobial activity, whereas the antimicrobial activity of the lactic acid fermented product extracts of Preparation Example 1 (Preparation Examples 1 to 5) was shown to be higher than that of the fermented product produced by lactic acid fermentation of Paeoniae . acnes and the fermentation broth of Comparative Example 2 showed no antimicrobial activity due to differences in fermentation products due to differences in the fermentation environment between the liquid phase and the solid phase.

Claims (6)

A solid phase product prepared by adding purified water to a powder of Baekjunju powder, and a solid phase fermented product obtained by inoculating Lactobacillus plantarum, which is a lactic acid bacterium, with an extract thereof, An antimicrobial composition for Onion Bacterium acnes .
The method according to claim 1,
Wherein the extract is water, ethanol or a mixed solvent thereof.
The method according to claim 1,
Wherein the lactic acid bacterium is Lactobacillus plantarum KCCM 80077.
4. The method according to any one of claims 1 to 3,
Wherein the composition is a cosmetic composition.
4. The method according to any one of claims 1 to 3,
Wherein said composition is a pharmaceutical composition.
6. The method of claim 5,
Wherein said composition has a therapeutic or prophylactic pharmacological effect of acne.

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