KR100817781B1 - Cosmetic composition comprising the propolis extract produced by extraction method of propolis using tocopherol - Google Patents

Abstract

The present invention relates to a cosmetic composition containing the extract obtained by the extraction method of propolis using tocopherol as an active ingredient.

The extract obtained by the extraction method of propolis using tocopherol contains the propolis active ingredients flavonoids and tocopherols, such as anti-inflammatory, antibacterial, antioxidant, cell regeneration, tissue regeneration, immuno-enhancing action and cell regeneration of tocopherol It also shows anti-aging and antioxidant effects.

Propolis extract obtained by the extraction method of the present invention can be usefully added to the cosmetic composition. In particular, the cosmetic composition containing the extract of propolis of the present invention exhibits the above-mentioned effects on the skin when applied to the skin, and may help to protect or relieve the skin, in particular, to prevent or alleviate acne, atopic dermatitis, and various dermatitis, and may help to heal wounds. have. In addition, it shows an excellent effect on skin moisturizing, there is no skin irritation can be usefully used as a cosmetic composition for skin protection.

 Propolis, Tocopherol, Cosmetics

Description

Cosmetic composition containing the propolis extract produced by extraction method of propolis using tocopherol}

The present invention relates to a cosmetic composition containing an extract obtained from the extraction method of propolis using tocopherol.

Propolis is made by mixing bees and enzymes with substances such as resins that honeybees extract from various plants for their survival and breeding. Propolis contains organic substances and minerals (inorganic salts). ) Is the most abundant with 104 kinds of ingredients. Among many components, minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, terpenes, etc. have an anticancer effect. In particular, there are more than 100 types of flavonoids, which can help you to improve your health.

In order to extract beneficial components from such propolis, many methods have been developed, and are usually dissolved and extracted in hydrophilic organic solvents such as acetone, acetic acid and lower alcohols (methanol, ethanol, isopropanol). However, due to the resin component of the propolis ingot, the water solubility of the extract is low, and there is a sticking and sticking property, so that it is not easy to add in the composition, and the content of the beneficial components in the extract is also low.

Korean Patent Publication No. 1995-2774 (published on March 12, 1995) (Solubilized Propolis Composition) extracts, filters and concentrates by adding ethanol to the propolis ingot to produce an extract or powder, and homogeneously mixed with cyclodextrin A composition containing solubilized propolis obtained by the present invention and its use as a beverage, cream, shampoo, soap, and other cosmetics are disclosed.

The known propolis extraction method is solubilized using cyclodextrin to be used in food, cosmetics, etc., but by using ethanol-containing solvent in the extraction of propolis, components such as minerals that are not dissolved in ethanol are lost. There are disadvantages.

Accordingly, the present inventors have made a thorough study to minimize the loss of the active ingredient contained in the propolis ingot and discarded sticky precipitate, and confirmed that the active ingredient is extracted by using tocopherol during propolis extraction and obtained from The present invention was completed by preparing the extract as a cosmetic composition.

The present invention is to provide a cosmetic composition containing the extract obtained by the extraction method of propolis using tocopherol.

The present invention provides a cosmetic composition containing the extract obtained by the extraction method of propolis using tocopherol.

According to an aspect of the present invention, there is provided a cosmetic composition containing an extract extracted by adding tocopherol to the propolis ingot.

That is, 1) adding tocopherol to the propolis ingot; 2) extracting the active ingredient by mixing and stirring the propolis ingot and tocopherol of the first step; And 3) provides a cosmetic composition containing 0.01 to 5% by weight of the extract obtained by the extraction method comprising the step of filtering the extract of step 2 to remove impurities.

In addition, according to another aspect of the present invention, the present invention provides a cosmetic composition containing an extract extracted by adding tocopherol to the sticky precipitate remaining after extraction from propolis ingot.

That is, 1) adding tocopherol to the propolis sticky precipitate; 2) extracting the active ingredient by mixing and stirring the sticky precipitate and tocopherol of the first step; And 3) provides a cosmetic composition containing 0.01 to 5% by weight of the extract obtained by the extraction method comprising the step of filtering the extract of the second step to remove impurities.

In addition, according to another aspect of the present invention provides a method for producing the cosmetic composition.

That is, 1) adding tocopherol to the propolis ingot; 2) extracting the active ingredient by mixing and stirring the propolis ingot and tocopherol of the first step; 3) filtering the extract of step 2 to remove impurities; And 4) containing the extract of step 3 in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition.

And 1) adding tocopherol to the propolis sticky precipitate; 2) extracting the active ingredient by mixing and stirring the sticky precipitate and tocopherol of the first step; 3) filtering the extract of step 2 to remove impurities; And 4) containing the extract of step 3 in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition.

Hereinafter, the present invention will be described in detail.

First, the extract extracted by adding tocopherol to the propolis ingot, which is an extract contained in the cosmetic composition of the present invention, is prepared through three steps as follows;

 1) adding tocopherol to the propolis ingot; 2) extracting the active ingredient by mixing and stirring the propolis ingot and tocopherol of the first step; And 3) filtering the extract of step 2 to remove impurities.

The first step is to add tocopherol to the propolis ingot, the propolis ingot can be used to extract the dried, not dried, or a mixture thereof. In addition, in the case of drying propolis ingot, according to the conventional drying method, it is used in the form of dry matter or in the form of finely divided or powder during extraction.

Then 0.1 to 5 times the weight of the prepared propolis ingot, preferably 0.2 to 2 times the volume (v / w) of tocopherol is added. The amount of tocopherol added is less than 0.1 times the weight of propolis, which is not preferable because the extraction rate of propolis decreases.Increasing the amount of tocopherol decreases the chewy properties of propolis, but it is convenient to use. If the above is used, the fat component in the extract is high, and the economic efficiency is low. The tocopherol may be produced and used, or a commercially available one may be used.

In the second step, the mixture of propolis ingot and tocopherol is slowly stirred and mixed at room temperature, that is, 10 to 35 ° C. When tocopherol is added to the propolis ingot, the propolis ingot is immediately dissolved in tocopherol, and since the propolis ingot is dissolved in tocopherol even at room temperature, it is not necessary to heat it. Extraction time is about 0.1 to 12 hours, but is not limited thereto. Extraction with stirring in this way can give a dark dark brown viscous liquid extract.

At this time, the obtained extract may be used as it is, but it is preferable to use the extract after removing the impurities such as beeswax, pollen through a third step of filtering the extract with a filterable cloth such as cotton cloth, filter paper, fine sieve. In addition, after step 3, the method may further include concentrating the filtrate.

The extract obtained through this preparation step contains the active ingredient and tocopherol of propolis, and contains 0.01 to 3% of flavonoids and 20 to 35% of tocopherols.

In addition, according to another aspect of the invention, the extract extracted by adding tocopherol to the propolis sticky precipitate as an extract contained in the cosmetic composition is prepared through three steps as follows;

1) adding tocopherol to the propolis sticky precipitate; 2) extracting the active ingredient by mixing and stirring the sticky precipitate and tocopherol of the first step; And 3) filtering the extract of step 2 to remove impurities.

The propolis sticky precipitate is a sticky precipitate remaining after extraction by a conventional method known in the propolis ingot, the sticky precipitate of the present invention can be obtained by extracting the propolis ingot by a known extraction method. Specifically, hot water extraction for about 6 hours to 10 days at an extraction temperature of room temperature to 100 ℃ with a low alcohol having a carbon number of C1 to C4, or a mixed solvent thereof of about 1 to 10 times the weight of propolis mass Liquid extracts and sticky precipitates can be obtained by extraction methods such as cold extraction, ultrasonic extraction or reflux cooling extraction. This sticky precipitate may be used as it is in one step, or lyophilized or ground to be used in one step.

The first step is to add tocopherol to the sticky precipitate, 0.1 to 5 times, preferably 0.2 to 2 times the volume of tocopherol to the weight of the sticky precipitate. When the amount of tocopherol added is less than 0.1 times the weight of propolis, the extraction rate of propolis decreases, and as the amount of added tocopherol increases, the sticky property of propolis decreases, which is convenient to use. Fatty ingredients in the extract is high, economical low. What was produced may be used for the said tocopherol, and a commercially available thing may also be used.

In the second step, the mixture of the sticky precipitate and the tocopherol of the first step is slowly stirred and mixed at room temperature, that is, 10 to 35 ° C. When tocopherol is added to the cohesive precipitate, the cohesive precipitate immediately dissolves in the tocopherol. In addition, the propolis sticky precipitate dissolves in tocopherol even at room temperature, and thus does not need to be heated. Extraction time is about 0.1 hours to 12 hours, but is not limited thereto. Extraction with stirring in this way can give a dark dark brown viscous liquid extract.

In the third step, the extract of the second step may be used after filtering impurities with a filterable cloth such as cotton cloth, filter paper, fine sieve, and the like to remove impurities such as beeswax and pollen. In addition, after the above-described three steps, it may further comprise the step of concentrating the filtrate.

The extract obtained through this preparation step contains the active ingredient and tocopherol of propolis, and contains 0.01 to 3% of flavonoids and 20 to 35% of tocopherol per g of propolis extract.

The extract obtained by the propolis extraction method using the tocopherol of the present invention can minimize the loss of the active ingredient of the propolis, contains a lot of various flavonoids showing the physiological activity of the propolis, and also contains tocopherol, The anti-inflammatory, antimicrobial, antioxidant, cell reactivation, tissue regeneration, immunopotentiation and cell regeneration, anti-aging and antioxidant effects of tocopherols are also shown.

Therefore, the propolis extract obtained by the extraction method of the present invention can be usefully used in addition to the cosmetic composition for exhibiting the above-mentioned effects. In this case, the propolis extract of the present invention is 0.01 ~ 0.01 to the total weight of the composition It is preferably contained in 5% by weight. The cosmetic composition of the present invention exhibits the above-mentioned effects on the skin when applied to the skin, which helps to prevent or alleviate the skin, in particular, acne, atopy, various dermatitis, and may help to heal the wound. In addition, the cosmetic composition of the present invention shows an excellent effect on skin moisturizing, there is no skin irritation can be usefully used as a cosmetic composition for skin protection.

In addition, the propolis extract obtained by the extraction method of the present invention can be easily added and used in the cosmetic composition as the steaming properties due to the resin component in the existing propolis is significantly lowered.

The cosmetic composition of the present invention is not particularly limited in the formulation, for example, softening cream, astringent makeup, nourishing cream, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, It may have a formulation known in the art such as packs, emulsions, lotions, ointments, gels, soaps or shampoos. In addition, other components in addition to the propolis extract of the present application may be selected and blended by those skilled in the art according to the formulation or purpose of use of other cosmetics. Preferred formulations of the cosmetic composition of the present invention are described below as preparation examples.

Hereinafter, preferred examples and preparation examples are provided to aid the understanding of the present invention. However, the following examples and preparations are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples and preparations.

Example 1 Preparation of Cosmetic Composition Containing Propolis Extract of the Present Invention

1-1) Preparation of Propolis Extract of the Present Invention 1

Tocopherol was added directly to the propolis ingot to extract the active ingredient of propolis.

Propolis ingot in the present invention was used in the beekeeping company of Dongsan-ri, Sangri-myeon, Goseong, Gyeongnam. 1 kg of the propolis ingot was chopped or pulverized, and 300 ml of natural α-tocopherol (Gyeongnam Pharmaceutical) was added thereto. Propolis ingots dissolve in tocopherol immediately upon addition of tocopherol. Propolis ingots were dissolved in tocopherol and extracted with stirring for at least 30 minutes at room temperature (20 ° C).

Then, suspended solids such as beeswax were removed from the extract by filtration with a cotton cloth, and finally, about 250 ml of crude propolis extract was obtained. The crude propolis extract thus obtained was viscous but significantly reduced in steaming properties and was a dark dark brown liquid extract. Within 1 g of the obtained extract, the extract contained about 2.5% of flavonoids and about 30% of tocopherols.

1-2) Preparation of Propolis Extract of the Present Invention 2

The active ingredient of propolis was extracted by directly adding tocopherol to the sticky precipitate generated when the propolis ingot was extracted with ethanol.

Propolis ingot in the present invention was used in the beekeeping company of Dongsan-ri, Sangri-myeon, Goseong, Gyeongnam.

0.45 kg of the propolis ingot was chopped or ground, and 1 liter of 80% ethanol was added thereto. Propolis was dissolved in ethanol solvent and extracted with stirring for at least 12 hours at room temperature (20 ° C).

Then, the suspended solids such as beeswax were removed from the extract, and 0.8 liters of crude liquid propolis and 60 g of solid sticky precipitate were obtained.

To 60 g of the above-mentioned sticky precipitate, 20 ml of natural α-tocopherol (Gyeongnam Pharm.) Was added, and the active ingredient in the precipitate was dissolved and extracted at room temperature with stirring at 25 ° C. As a result, 25 ml of liquid extract was obtained, with a dark viscous slightly viscous and remarkably reduced steaming property.

Within 1 g of the obtained extract, the extract contained about 0.6-0.7% of flavonoids and about 25-30% of tocopherols.

In the propolis extraction method of the present invention according to the above Examples 1-1) and 1-2), the solubility of propolis agglomerates and sticky precipitates with respect to tocopherol is high, and almost no amount is discarded during the extraction process. In addition to minimizing the loss of the active ingredient, the final extract contains a lot of various flavonoids that exhibit the physiological activity of the propolis. The extract itself will also contain tocopherols.

In addition, due to the resin component in the propolis extract is significantly lowering the steaming characteristics, it is easy to use, it is easy to add to compositions such as cosmetics.

1-3) Preparation of Cosmetic Composition Containing Extract of Propolis of the Present Invention

Using the propolis extract of Example 1-1) or Example 1-2) to prepare a cosmetic composition for skin protection as follows.

(A) lotion

ingredient content Extract of Example 1-1 0.5 wt% Glyceryl Stearate SE 1.5 wt% Stearyl alcohol 1.0 wt% Polysorbate 60 1.2 wt% Sorbitan Stearate 0.4 wt% Liquid paraffin 10.0 wt% vegetable oil 5.0 wt% Squalene 2.0 wt% Carboxy Vinyl Polymer 0.14 wt% glycerin 3.0 wt% 1,3-butylene glycol 3.0 wt% Sodium hyaluronate 0.15 wt% Triethanolamine 0.14 wt% antiseptic Quantity Pigment Quantity Spices Quantity Purified water Balance added up to 100% by weight

The lotions were prepared according to conventional methods in the cosmetic preparation field for the preparation of lotions using the above ingredients.

(B) Nutrition Cream

ingredient content Extract of Example 1-2) 1.0 wt% Lipophilic monostearylglycerin 2.0 wt% Stearyl alcohol 2.5 wt% Stearic acid 1.3 wt% Polysorbate 60 1.5 wt% Sorbitan stearate 0.5 wt% Liquid paraffin 5.0 wt% Sodium magnesium silicate 3.0 wt% glycerin 2.5 wt% Triethanolamine 1.3 wt% Sodium hyaluronate 0.5 wt% antiseptic Quantity Pigment Quantity Spices Quantity Purified water Balance added up to 100% by weight

Using the above ingredients, the nourishing cream was prepared according to a conventional method in the cosmetic manufacturing field for the preparation of the nourishing cream.

(C) soap

ingredient content Extract of Example 1-2) 1.0 wt% Soap base 98.5 wt% Spices 0.5 wt%

Soaps were prepared according to conventional methods in the art of making cosmetics for the production of soaps using the above ingredients.

1-4) Antimicrobial Effect Experiment of Propolis Extract of the Present Invention

The antimicrobial activity of the extract prepared above was measured as follows.

The antimicrobial activity is Bacillus subtilis , Staphylococcus aureus , Streptomyces Streptomyces murius , Aspergillus niger , Propionibcterium acnes , A total of five strains were used.

After incubating the bacteria in a liquid medium, the number of bacteria was measured and plated in the same amount in a plate count Agar (PCA) solid medium and inoculated. Propionibacterium Acnes used brain heart infusion (BHI) medium. Incubated for 48 hours at 37 ℃ anaerobic.

Further, the propolis extract obtained above was diluted to 0.1%, 1%, 3%, and 5% in ethanol in a sterilized paper disc (0.8 cm in diameter), and 30 µl was added thereto, and left for 30 minutes to evaporate ethanol. It was placed on a solid medium where each bacterium was smeared and further anaerobicly cultured for 24 hours (bacteria) or 48 hours (fungus). After incubation, the diameter of the clear ring (growth-lowering ring) generated in the medium was measured, and antimicrobial activity was measured.

The experimental results are shown in Table 4.

           Transparent ring size unit: cm 0.1% 1.0% 3.0% 5.0% Bacillus subtilis 0.5 1.5 2.0 3.0 Staphylococcus aureus 1.0 2.5 3.5 4.0 Streptomyces murius 1.0 2.0 3.0 4.0 Aspergillus niger 0.5 1.5 2.5 3.0 Propionibcterium acnes 0.5 1.0 2.0 2.5

Looking at the results of Table 4, when the propolis extract of the present invention at a dilution concentration of 0.1% to 5.0%, it is confirmed that the growth of bacteria containing propolis is suppressed to form a transparent ring.

Therefore, it can be seen that the cosmetic composition containing the propolis extract of the present invention can exhibit an excellent antimicrobial effect.

1-5) Sensory Evaluation of Cosmetic Composition Containing Propolis Extract of the Present Invention

In the present experimental example, the cosmetic composition (lotion, nourishing cream) prepared in Example 1-3) was subjected to sensory tests by ranking method for 60 women in their 20s to 50s. As a comparative example, a nutrition cream containing 7% by weight of propolis extract was used in the cosmetic composition. Twenty people were assigned to each composition, and each composition was used twice a day for one week, followed by a 9-point scale (1 is very bad, 2 to 3 is bad, 4 to 6 is normal, and 7 to 8 is good, 9 is very good) and the average value is shown in Table 5.

incense Skin Moisturizing Flexibility (Soft) Skin irritation Feeling Example 1-3) Lotion 8 8 8 8.5 Example 1-3) Nutrition Cream 8.5 8 8.5 7.5 Comparative example 3.5 6 5.5 5

 In the results of Table 3, in the case of the propolis extract-containing cosmetic composition of the present invention, the characteristic irritant odor was reduced, and received good evaluation for skin moisturizing and softness (softness). There was no skin irritation. The use of the lotion and nourishing cream of the present invention was excellent, but the nourishing cream of the comparative example was greasy and had a low evaluation because of its greasy and slippery feeling.

As described above, the extract obtained by the propolis extraction method using the tocopherol of the present invention can minimize the loss of the active ingredient of the propolis, contains a lot of various flavonoids showing the physiological activity of the propolis, tocopherol It also contains anti-inflammatory, antibacterial, anti-oxidant, cell reactivation, tissue regeneration, immunopotentiating and tocopherol cell regeneration, anti-aging and antioxidant effects of propolis.

Propolis extract obtained by the extraction method of the present invention can be usefully added to the cosmetic composition for exhibiting the above effects. In particular, the cosmetic composition containing the extract of propolis of the present invention exhibits the above-mentioned effects on the skin when applied to the skin, and may help to protect or relieve the skin, in particular, to prevent or alleviate acne, atopic dermatitis, and various dermatitis, and may help to heal wounds. have. In addition, it shows an excellent effect on skin moisturizing, there is no skin irritation can be usefully used as a cosmetic composition for skin protection.

Claims (6)

delete delete delete delete 1) adding tocopherol to the propolis ingot; 2) extracting the active ingredient by mixing and stirring the propolis ingot and tocopherol of the first step; 3) filtering the extract of step 2 to remove impurities; And 4) containing the extract of step 3 in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition. 1) adding tocopherol to the propolis sticky precipitate; 2) extracting the active ingredient by mixing and stirring the sticky precipitate and tocopherol of the first step; 3) filtering the extract of step 2 to remove impurities; And 4) containing the extract of step 3 in an amount of 0.01 to 5% by weight based on the total weight of the cosmetic composition.