KR102057022B1 - Cosmetic composition and manufacturing method for the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil, tocopheryl acetate, aqueous arginine solution, moisturizer, green tea extract and solvent, and a preparation method thereof. Through this, the present invention includes fermented glycolipids, while omitting the use of sterilizing-preservatives, synthetic surfactants or reducing the amount of use, while achieving a remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity, moisturizing effect To improve the skin soothing effect and usability, and to provide a cosmetic composition with reduced acid odor and a method for producing the same.

Description

Cosmetic composition and its manufacturing method {COSMETIC COMPOSITION AND MANUFACTURING METHOD FOR THE SAME}

The present invention relates to a cosmetic composition comprising the fermented glycolipid and a method for producing the same.

Cosmetics are a product that gives people beauty and abundance and is becoming an essential product in daily life. In addition, efforts to secure safety as a product that is used on the skin multiple times daily and used for a long time are emphasized.

Accordingly, the cosmetic composition is added to the sterilization-preservatives, synthetic surfactants and the like in order to prevent defects such as deterioration, deterioration or contamination of the product. However, bactericidal-preservatives, synthetic surfactants, etc. may cause factors that cause cytotoxicity and contact allergy in some people, so there is a problem that it is dangerous to be used universally.

The bactericidal-preservatives that have recently been used for attention include parabens, phenoxy ethanol, imidazolidinyl urea, clophenesin, and the like, and the synthetic surfactants are sodium lauryl sulfate, sodium laureth sulfate, and TEA. Lauryl sulfate and the like.

However, it is not easy to prevent the deterioration and decay of the cosmetic composition without including the above components. Accordingly, efforts have been made to replace sterilization-preservatives, synthetic surfactants, and the like using various kinds of naturally-derived materials or extracts, but it is difficult to realize a high degree of effectiveness required at an industrial scale.

Accordingly, there is an increasing need for the development of a cosmetic composition that can replace sterilization-preservatives, synthetic surfactants, and the like, and is excellent in its effectiveness and sufficient for industrial mass production, and has properties that are advantageous for application to cosmetics. .

One object of the present invention is to include fermented glycolipids, thereby omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, while achieving remarkably excellent antibacterial activity, surfactant activity, emulsion stability and antioxidant activity, and moisturizing. The present invention provides a cosmetic composition having improved effect, soothing effect and usability, and reduced acid odor and a method for producing the same.

One embodiment of the present invention relates to a cosmetic composition comprising fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil, tocopheryl acetate, aqueous arginine solution, moisturizer, green tea extract and solvent.

The cosmetic composition is 0.1% to 10% by weight of fermented glycolipid, 0.01% to 1% by weight of ceramide, 0.005% to 0.5% by weight of phytosphingosine, 0.1% to 30% by weight of camellia seed oil, sunflower seed oil 0.1 wt% to 10 wt%, 0.01 wt% to 0.8 wt% tocopheryl acetate, 0.1 wt% to 10 wt% of aqueous arginine solution, 0.1 wt% to 30 wt% of moisturizer, 0.1 wt% to 5 wt%, and green tea extract A negative solvent may be included.

The fermented glycolipid may be obtained by culturing Candida bombbicola on a culture substrate comprising vegetable oil, glucose, rice bran and medium.

The vegetable oil may include one or more of palm oil, palm oil and canola oil.

The culture substrate may include 5% to 15% by weight of vegetable oil, 5% to 15% by weight of glucose, 1% to 5% by weight of rice bran, and 65% to 80% by weight of medium.

Another embodiment of the present invention is after dissolving fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil and tocopheryl acetate in a solvent, and then adding an aqueous alginine solution, a warm dissolved moisturizer and a green tea extract. It relates to a cosmetic composition manufacturing method comprising the step of formulating, by high speed stirring.

The method of preparing the cosmetic composition further comprises the step of culturing Candida bombbicola on a culture substrate comprising vegetable oil, glucose, rice bran and medium to prepare a culture solution, and then removing the culture solution from impurities and extracting ethanol to obtain fermented glycolipids. It may include.

The impurity removal may include cell sediment removal for centrifugation of the culture, residual oil removal for hexane extraction, and odor removal for permeation to activated carbon and celite.

The present invention includes a fermented glycolipid, thereby omitting or reducing the use of bactericidal preservatives and synthetic surfactants, while achieving remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity, moisturizing effect, and skin soothing effect. The present invention provides a cosmetic composition having improved effect and usability and reduced acid odor and a method for producing the same.

1 is a photograph showing the results of visual evaluation of the emulsion stability of the fermented glycolipids prepared in Preparation Examples 4 to 5 and Comparative Examples 1 to 3 of the present invention.
2 to 6 are graphs showing the results of evaluation of emulsion stability absorbance of the fermented glycolipids prepared in Preparation Examples 4 to 5 and Comparative Examples 1 to 3 of the present invention.
Figure 7 is a graph showing the surface tension measurement results for the fermented glycolipid prepared in Preparation Examples 1 to 3 of the present invention.
8 is a graph showing the results of the evaluation of the emulsion stability over time for the fermented glycolipids prepared in Preparation Examples 1 to 3 of the present invention.

One embodiment of the present invention relates to a cosmetic composition comprising fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil, tocopheryl acetate, aqueous arginine solution, moisturizer, green tea extract and solvent.

Through this, the present invention includes fermented glycolipids, while omitting the use of sterilizing-preservatives, synthetic surfactants or reducing the amount of use, while achieving a remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity, moisturizing effect To improve the skin soothing effect and usability, and to provide a cosmetic composition with reduced acid odor and a method for producing the same.

In particular, the present invention is the ceramide and phytosphingosine complex acts on the fermentation glycolipid, the liquid crystal emulsification in the solvent containing a camellia seed oil, sunflower seed oil, tocopheryl acetate, an arginine aqueous solution, a moisturizer and green tea extract You can implement the effect. In this case, the effect of improving the absorption rate of the cosmetic composition on the skin is excellent.

The fermented glycolipid is a kind of biosurfactant prepared from living organisms, and thus has no high cytotoxicity, and thus has high stability to the human body. In addition, it is possible to implement excellent surface-activity as a glycolipid without causing contact allergy like artificial synthetic materials.

Specifically, the fermented glycolipid may be obtained by culturing Candida Bombi-coli bacteria on a culture substrate comprising vegetable oil, glucose, rice bran and medium. In this case, the HLB value (Hydrophile-Lipophile Balance) satisfies the degree to which the surfactant activity is improved, and the antibacterial effect (specifically, the antibacterial effect against acne bacteria), the moisturizing effect and the skin soothing effect are improved. Glycolipids can be obtained.

The vegetable oil is used as a substrate of microorganisms and is used as a raw material for fermentation glycolipid biosynthesis. The fermented glycolipids prepared as described above may implement surfactant activity, antimicrobial activity, emulsification and emulsion stability, and antioxidant activity according to the properties of the vegetable oil.

More specifically, the vegetable oil may include one or more of palm oil, palm oil, canola oil. In this case, the fermentation glycolipid produced from the vegetable oil of the above example can be improved to the extent that the hydrophobic portion of the fermentation glycolipid is converted to a more suitable HLB value.

In one embodiment, when palm oil is used as the vegetable oil, it is possible to further improve the emulsifying power, emulsification stability, and antioxidant capacity of the fermented glycolipid obtained.

In another embodiment, the use of palm oil as the vegetable oil can further improve the emulsifying power and emulsification stability of the fermented glycolipid obtained.

In another embodiment, when using canola oil as the vegetable oil, it is possible to further improve the emulsification power, emulsification stability, and antimicrobial activity of the fermented glycolipid obtained.

The glucose is used as a substrate of the microorganisms as a raw material for the fermentation glycolipid biosynthesis, and can also be used as an energy source of the microorganism to further improve the biosynthesis efficiency of the fermentation glycolipid. The fermented glycolipid prepared as described above may implement the surface active ability, antimicrobial activity, emulsifying power and emulsion stability, antioxidant activity and the like according to the degree of hydrophilic group content of the fermented glycolipid converted from glucose.

The rice bran can be used as an energy source of microorganisms to further improve the biosynthetic efficiency of fermented glycolipids. In addition, by providing additional mineral components, vitamins, minerals, and the like contained in the rice bran can further improve the culture degree of the microorganism, through the culture is produced rice bran fermentation extract. The rice bran fermentation extract may be included in obtaining the fermented glycolipid may be further contained in the cosmetic composition of the present invention.

The medium may be used as a culture source of microorganisms to stably improve the biosynthetic efficiency of fermented glycolipids. The medium may be formulated directly or may be a commercial liquid medium, and is not particularly limited as long as it is suitable for culturing Candida Bombicola.

Candida Bombicola (Candida Bombicola) is a microorganism capable of biosynthesis of glycolipids based on the oil component as a substrate, its kind is not particularly limited. When using Candida Bombiola bacteria can achieve better culture, higher fermentation glycolipid yield. The culture substrate may include 5% to 15% by weight of vegetable oil, 5% to 15% by weight of glucose, 1% to 5% by weight of rice bran, and 65% to 80% by weight of medium. Within this content range, better culture, higher fermentation glycolipid yield can be achieved by the culture substrate.

The inoculation amount of the Candida Bombi-coli bacteria is not particularly limited, but may be inoculated in an amount of 2 parts by weight to 5 parts by weight based on 100 parts by weight of the culture substrate for fermentation glycolipid production. Within this content range, good fermentation glycolipid yield can be achieved.

The culture temperature of the Candida Bombi-Cola bacteria is not particularly limited, but may be, for example, 25 ° C to 35 ° C. Within this content range, good fermentation glycolipid yield can be achieved.

The culture solution is, for example, recovered from the upper portion after standing and fractionated with hexane to remove the residual oil, and then added by ethanol, activated carbon and celite twice the culture supernatant and filtered after stirring for 1 hour to 2 hours The product can be concentrated to yield fermented glycolipids. In this case, the availability and recovery rate of the fermented glycolipid may be further improved.

The fermentation glycolipid may be included in 0.1 wt% to 10 wt%. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

The ceramides (Ceramides) and phytosphingosine penetrates between the keratinocytes of the skin epidermis, increases the binding force between cells, and improves skin immunity through improvement of skin barrier function. In addition, the liquid crystal emulsification state of the lamellae liquid crystal structure of intercellular lipids can be realized by complexing with the above-mentioned fermented glycolipid, and in this case, it further activates the skin barrier function and further improves the absorbency of the active ingredient when used in cosmetic compositions. .

Specifically, the ceramide may act on the cell membrane to replenish the natural lipids of the cell membrane, protect the skin, maintain moisture, and improve the protective effect against bacteria and environmental pollutants.

More specifically, the ceramide may be used in a fine emulsified state or in the form of nanoparticles (NP), and in this case acts on the skin to further enhance the moisturizing effect, antioxidant effect, absorption effect, etc. Skin elasticity enhancing effect can be achieved.

The ceramide may be included in an amount of 0.01 wt% to 1 wt%. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

Specifically, the phytosphingosine acts on the stratum corneum of the skin to protect the skin, improve the defense effect against bacteria and environmental pollutants, moisturizing effect, antioxidant effect, antibacterial effect (eg, cadmium bacteria Antimicrobial effect on), anti-inflammatory effect (for example, anti-inflammatory effect on redness), etc., while further increasing the effect can be realized that does not cause side effects even in long-term use.

The phytosphingosine may be included in 0.005% by weight to 0.5% by weight. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

The camellia seed oil is excellent in preventing their rancidity while supplying unsaturated fatty acids and the like. In addition, by supplying a large amount of active ingredients, such as oleic acid, oleic acid triglycerides to improve the skin barrier function, prevent moisture loss, relieve fine wrinkles, and implements excellent antimicrobial, skin elasticity enhancement and antioxidant effect.

The camellia seed oil may be included in an amount of 0.1 wt% to 30 wt%. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

The sunflower seed oil provides a lipid component having properties similar to the lipid component inside the skin. This prevents water from evaporating from the epidermis when applied to the skin, quickly repairs broken skin barriers, and enhances moisturizing effects. In addition, by supplying a large amount of active ingredients such as linoleic acid to improve the skin barrier function, prevent percutaneous moisture loss, relieve fine wrinkles, and implements excellent antimicrobial, skin elasticity enhancement and antioxidant effect.

The sunflower seed oil may be included in an amount of 0.1 wt% to 10 wt%. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

The camellia seed oil contains an excessive amount of oleic acid, which may adversely affect oily skin or acne skin, but in the cosmetic composition of the present invention, sunflower seeds are used by combining the camellia seed oil and the sunflower seed oil. The linoleic acid of the oil can realize the effect of preventing such adverse effects.

The tocopheryl acetate is a fat-soluble vitamin E acetate (vitamin E acetate), it has an excellent antioxidant capacity to protect the skin from harmful oxygen, implement skin conditioning effect, can supply tocopherol to the skin cell membrane to prevent damage caused by oxidation have.

The tocopheryl acetate may be included in an amount of 0.01 wt% to 0.8 wt%. Within this range, while omitting or reducing the use of sterilizing-preservatives and synthetic surfactants, the effect of implementing remarkably excellent anti-corruption, antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved. .

Arginine (Arginine) is an alkaline essential amino acid present in nature, which improves regenerative effects such as anti-aging and wound healing of the skin, and may act on the skin to promote collagen synthesis. In addition, the fermented glycolipid, the vegetable oil-derived site in the fermented glycolipid, and the complex action with the vegetable oil to form a salt, thereby further improving the surfactant effect, it can further enhance the skin soothing effect.

The arginine aqueous solution may be included in an amount of 0.1 wt% to 10 wt%. Specifically, the aqueous alginine solution may be in a concentration of 0.05% to 10%, for example, a mixture of 0.1 to 5% by weight of arginine and 5% to 9.9% by weight of purified water. More specifically, the solid content of the arginine may be 0.1% to 3% by weight of the total cosmetic composition. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

The moisturizing agent further improves skin moisturizing and skin soothing effects in the cosmetic composition of the present invention, and may specifically include betaine, glycerin, and the like.

The trimethylglycine is a small trimethylated aminoacid present in the form of a positive ion at neutral pH, and water and other active ingredients in the cosmetic composition can easily form hydrogen bonds and improve solubility. have. In addition, as well as moisturizing effect can be added anti-inflammatory, anti-inflammatory, anti-aging, whitening and the like.

The glycerin implements a skin conditioning effect and may further improve skin moisturizing and skin soothing effects.

The moisturizing agent may be included in an amount of 0.1 wt% to 30 wt%. Within this range, the effect of implementing a remarkably excellent skin moisturizing effect, skin soothing effect, emulsion stability and antioxidant activity can be further improved.

The green tea extract is extracted from the leaves of Camellia sinensis and the green tea extract acts on the skin as a natural antioxidant material to implement a cell protection function, protect the skin from free radical damage, UV absorption effect, antioxidant effect. In addition, by supplying methylxanthines, it stimulates skin microcirculation, positively affects skin health, reduces skin inflammation, inhibits collagen breakdown enzymes, and inhibits collagenase. Enhance collagen synthesis and maintain skin elasticity, and deodorant effect can further prevent the occurrence of rancidity.

The green tea extract may be included in an amount of 0.1 wt% to 5 wt%. Within this range, it is possible to implement more excellent antioxidant capacity, anti-acid patch effect, emulsion stability improving effect.

The solvent serves to stabilize the formulation and to improve the applicability, spreadability, and the like of the cosmetic composition to the skin. In addition, it may serve to supply additional moisture when applied to the skin.

Specifically, the solvent may include one or more of purified water and butylene glycol. In this case, more excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be achieved.

In addition, the solvent may further comprise a thickening agent as an additional component. The thickener may be, for example, carbomer or the like. In this case, the content of the thickener may be 0.1% to 2% by weight in the cosmetic composition.

The solvent may be included in the balance, specifically, 65% to 90% by weight, except for the above-mentioned components. Within this range, while omitting or reducing the use of bactericidal-preservatives and synthetic surfactants, the effect of implementing remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity can be further improved.

Another embodiment of the present invention is after dissolving fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil and tocopheryl acetate in a solvent, and then adding an aqueous alginine solution, a warm dissolved moisturizer and a green tea extract. It relates to a cosmetic composition manufacturing method comprising the step of formulating, by high speed stirring.

Each component included in the cosmetic composition of the present invention and the content thereof are the same as described above.

The method of preparing the cosmetic composition further comprises the step of culturing Candida bombbicola on a culture substrate comprising vegetable oil, glucose, rice bran and medium to prepare a culture solution, and then removing the culture solution from impurities and extracting ethanol to obtain fermented glycolipids. It may include.

The step of obtaining the fermented glycolipid is not particularly limited, and may include, for example, inoculating Candida bombbicocci into a culture substrate including vegetable oil, glucose, rice bran and medium to prepare a culture solution. In this case, the culture solution may include the remaining culture substrate components, fermented glycolipids, and Candida bombbiola, which are used as substrates. The culture solution may be used as it is, but may be used after removing impurities to enhance usability.

The impurity removal may include cell sediment removal for centrifugation of the culture, residual oil removal for hexane extraction, and odor removal for permeation to activated carbon and celite. In this case, the purity and stability of the fermented glycolipid can be further improved, which may be more advantageous for application to industrial mass production processes. The impurity removal may be performed simultaneously or separately in any order.

In one embodiment, preparing the fermented glycolipid may include adding ethanol, activated carbon, and celite to the culture, followed by stirring for 1 hour, and then performing filter filtration. In this case, the availability and recovery rate of the fermented glycolipid may be further improved.

Specifically, the method for preparing a cosmetic composition is completely dissolved by high speed stirring while heating the solvent, followed by cooling. After dissolving, an aqueous arginine solution, a warm dissolved warm moisturizer and a green tea extract may be added, and then formulated by high speed stirring.

The elevated temperature may be, for example, 60 ° C to 80 ° C, and the cooling temperature may be, for example, 40 ° C to 55 ° C. Within this range, the formulation of the cosmetic composition may be more uniform, and the high speed stirring efficiency may be more excellent.

The high speed agitation may be performed through a homo mixer, a paddle mixer, or the like, for example, at a speed of at least 3000 rpm for a homo mixer, at a speed of at least 500 rpm for a paddle mixer, for example, for 10 minutes to 50 minutes. Within this range, the formulation of the cosmetic composition may be more uniform, and the high speed stirring efficiency may be more excellent.

The cosmetic composition may have a formulation for application to various kinds of cosmetics and the like. The formulation is not particularly limited, and for example, an additional excipient may be added to the cosmetic composition to prepare a mixture, and the mixture may be a lotion, essence, foundation, powder, lotion, cream, pack, gel, ointment, patch, spray, It can be formulated with either a mask sheet or a detergent.

Example

Hereinafter, the configuration and operation of the present invention through the preferred embodiment of the present invention will be described in more detail. However, this is presented as a preferred example of the present invention and in no sense can be construed as limiting the present invention.

Preparation Example 1

Candida Bombicola (ATCC 22214) strain was used to produce fermented glycolipids.

Specifically, 10% by weight of glucose and 10% by weight of canola oil (oil) and the remaining medium were sterilized in 3% by weight of rice bran. After inoculating 2% of yeast (Candida Bombbi-Cola) on the sterilized medium, it was incubated at 30 ° C for 7 days.

After the culture solution was recovered, the cells were allowed to stand for a certain time to settle the cells, and the upper layer was collected, and then fractionated by adding 1/5 hexane (hexane) to remove residual oil. Thereafter, ethanol, activated carbon (2% w / v), and celite (5% w / v) of the culture supernatant were added thereto, stirred for 1 to 2 hours, and concentrated to obtain a fermented glycolipid by concentrating the obtained product.

Preparation Examples 2 to 3

Fermented glycolipids were extracted in the same manner as in Preparation Example 1, except that palm oil (Preparation Example 2) and palm oil (coconut oil, Preparation Example 3) were used instead of canola oil, respectively.

Preparation Example 4

5% by weight of fermented glycolipid prepared in Preparation Example 1, 5% by weight of mineral oil, 1.5% by weight of arginine and 88.5% by weight of purified water were mixed and emulsified to prepare a sample, and then emulsification activity evaluation was performed.

Preparation Example 5

After mixing and emulsifying 5% by weight of fermented glycolipid prepared in Preparation Example 1, 1% by weight of mineral oil, 1.5% by weight of arginine and 92.5% by weight of purified water, a sample was prepared, and then emulsifying activity evaluation was performed.

Example 1

73.39% by weight of purified water, 8% by weight of butylene glycol and 0.25% by weight of carbomer were added to the main tank to prepare a first solution in a completely dissolved state. Thereafter, the temperature of the first solution was heated to 75 ° C.

 In a separate oil tank, 3% by weight of Camellia seed oil, 0.5% by weight of sunflower seed oil, 0.04% by weight of ceramide NP, 0.02% by weight of phytosphingosine and 0.2% by weight of tocopheryl acetate, 6.6% by weight of the aqueous solution (0.6% by weight of solid alginine and 6% by weight of purified water) and 6% by weight of glycerin were added thereto, and 1% by weight of green tea extract was added thereto to completely dissolve the second solution.

After preparing the mixture by adding the second solution to the first solution, the mixture was stirred for 30 minutes while maintaining 75 ° C, the mixture was cooled slowly to 60 ° C with sufficient stirring, and then stirred at a high speed of a homomixer at 3000 rpm for 30 minutes again. Slowly cooling with stirring to prepare a cosmetic composition.

Comparative Examples 1 to 3

A sample was prepared in the same manner as in Preparation Example 4, except that the composition was changed as in Table 1 below. Samples of Comparative Examples 1 to 3 were used for the evaluation of the emulsion activity together with Preparation Examples 4 to 5.

Ingredient Name Preparation Example 4 Preparation Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Purified water 88.5 92.5 90 90 94 Mineral oil 5 One 5 5 One Fermented Glycolipids 5 5 - 5 - Arginine 1.5 1.5 - - - lecithin - - 5 - 5 Total (wt%) 100 100 100 100 100

Comparative Examples 4 to 7

A cosmetic composition was prepared in the same manner as in Example 1, except that the composition was changed as in Table 2 below when preparing the cosmetic composition.

Ingredient Name Example 1 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Purified water 73.39 73.39 73.45 73.59 74.39 Butylene glycol 8 8 8 8 8 Carbomer 0.25 0.25 0.25 0.25 0.25 Camellia Seed Oil 3 - 3 3 3 Sunflower Seed Oil 0.5 - 0.5 0.5 0.5 Macadamia Seed Oil - 3 - - - Avocado Oil - 0.5 - - - Ceramide NP 0.04 0.04 - 0.04 0.04 Phytosphingosine 0.02 0.02 - 0.02 0.02 Tocopheryl Acetate 0.2 0.2 0.2 0 0.2 Fermented Glycolipids One One One One One Purified water
(Content in Arginine Aqueous Solution) 6 6 6 6 6 Arginine
(Solid Content in Arginine Aqueous Solution) 0.6 0.6 0.6 0.6 0.6 glycerin 6 6 6 6 6 Green Tea Extract One One One One 0 Total (wt%) 100 100 100 100 100

<Property evaluation method>

For the samples prepared in Preparation Examples, Examples and Comparative Examples, the emulsifying activity, antimicrobial activity and user satisfaction were evaluated by the following methods.

1) Emulsification Activity Visual Evaluation

The samples of Preparation Examples 4 to 5 and Comparative Examples 1 to 3 were each emulsified in the same manner, and then left to stand at room temperature for 3 days and then visually observed to evaluate the degree of emulsifying activity.

The turbidity is increased by emulsification, and the strong yellowness of the sample is about visually identified, and when no layer separation occurs, the degree of emulsification activity is expressed as 'good'. In addition, it was difficult to visually identify that the strong yellow color of the sample was pale due to emulsification, and the color of the upper layer and the lower layer was marked as 'poor' when a different layer separation was observed.

2) Evaluation of Emulsifying Activity Absorbance

1 ml of each of the samples of Preparation Examples 4 to 5 and Comparative Examples 1 to 3 was collected, and then diluted 100-fold. For each diluted sample, the emulsion activity was measured for absorbance at 620 nm using a spectrophotometer (UV-160A, SHIMADZU Co.,).

3) Antibacterial activity evaluation

Evaluation of the antimicrobial activity of the samples of Preparation Examples 4 to 5 and Comparative Examples 1 to 3 was carried out the bacterial count test in accordance with the microbial limit criteria and test method guidelines of cosmetics distributed by the Food and Drug Administration.

Specifically, 1 mL of the pretreatment sample solution was smeared on the surface of the modified retinal agar medium previously hardened in a 9-10 cm diameter Petri dish, sterilized, and 15 mL of medium cooled to 45 ° C. was mixed well. At least two plates per sample were prepared and incubated at 30 to 35 ° C. for at least 48 hours, and the plate having the maximum colony count among the plates was selected to visually evaluate the degree of antimicrobial activity.

4) User Satisfaction Rating

The cosmetic compositions prepared in Example 1 and Comparative Examples 4 to 7 were evaluated for moisturizing feeling, soothing effect, odor, and feeling through the following methods, respectively, through 12 members (4 males and 8 females).

A. Evaluation of moisturizing feeling (5 times a day): The evaluation method was used once a day, taking about 3g of sample and spreading evenly on the face. The total scores were summed to represent the mean value.

1 point: very dry, 2 points: dry, 3 points: normal, 4 points: moist, 5 points: very moist

B. Evaluation of sedation effect (5 times a day): The evaluation method was used once a day, taking about 3g of the sample and evenly applied to the face. The total scores were summed to represent the mean value.

1 point: very stimulating, 2 points: irritating, 3 points: moderate, 4 points: comfort, 5 points: calming

C. Evaluation of Odor (One Sample After 30 Days): After 14 days of preparation, an evaluation method was applied to the back of the hand and smell test was performed. The total scores were summed to represent the mean value.

1 point: high rancid odor, 2 points: rancid odor, 3 points: very low rancid odor, 4 points: almost no rancid odor, 5 points: no smell

D. Evaluation of the feeling of use (five times a day): The evaluation method was used once a day, taking about 3g of the sample and evenly applied to the face. The total scores were summed to represent the mean value.

1 point: very sticky, 2 points: sticky, 3 points: non sticky but oily, 4 points for comfort, 5 points for calming effect

<Evaluation result>

1) Emulsification Activity Visual Evaluation

Evaluation results are shown in FIG. 1 and Table 3 below. As a result of the evaluation of emulsifying activity, Preparation Example 4 and Preparation Example 5 were able to confirm the result of 'excellent' emulsifying activity, whereas Comparative Examples 1 to 3, which do not contain fermented glycolipid or do not contain arginine, are separated from each other or emulsified. It can be seen that the yellow color of the sample appeared strongly because it did not proceed homogeneously.

2) Evaluation of Emulsifying Activity Absorbance

Evaluation results are shown in FIGS. 2 to 6 and Table 3 below. As a result of the evaluation of the emulsion activity absorbance, Preparation Example 4 and Preparation Example 5 were excellent in the degree of emulsification and dispersion degree, it is possible to confirm the high absorbance of 0.25A or more, whereas Comparative Examples 1 to 3 containing no fermented glycolipid or arginine It can be seen that the layer separation occurs or the emulsification does not proceed homogeneously, indicating a relatively low absorbance of less than 0.25A.

3) Antibacterial activity evaluation

The evaluation results are shown in Table 3 below. As a result of the evaluation of antimicrobial activity, it was confirmed that the preparation examples 4 and 5 realized excellent antimicrobial activity of 5 cfu or less even though no artificial preservative was included, but Comparative Examples 1 to 3 were found to have a relatively low antimicrobial activity.

Preparation Example 4 Preparation Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Emulsifying Activity Visual Evaluation Great Great Bad Bad Bad Emulsifying Activity Absorbance (Unit: A) 0.27 0.30 0.12 0.13 0.22 drawing 2 3 4 5 6 Antimicrobial activity
(cfu) 0 3 11 6 12

4) User Satisfaction Rating

Example 1 is a cosmetic composition comprising fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil, tocopheryl acetate, aqueous arginine solution, moisturizer, green tea extract and solvent through the results of Table 4 below. Moisturizing effect, soothing effect, rancidity prevention effect, feeling of all the tests were confirmed that the satisfactory results.

On the other hand, Comparative Example 4 using macadamia seed oil and avocado oil instead of camellia seed oil and sunflower seed oil of Example 1 showed a lower score than Moisturizing effect, soothing effect, rancidity prevention effect, and feeling in comparison with Example 1 In particular, it was confirmed that the results were not satisfactory in moisturizing feeling and feeling of use (feeling frustrating).

In addition, Comparative Example 5, which does not contain ceramide and phytosphingosine, showed a lower score than Example 1 in moisturizing effect, soothing effect, rancidity prevention effect, and feeling, and in particular, unsatisfactory result in skin soothing effect, etc. It was confirmed that the.

In addition, Comparative Example 6, which does not include tocopheryl acetate, showed a lower score compared to Example 1 in the moisturizing effect, soothing effect, anti-oxidation effect, and feeling of use, and particularly, anti-oxidative effect of anti-oxidative activity (which is rancidated by low antioxidant activity) It was confirmed that the results were not satisfactory.

In addition, Comparative Example 7 that does not include green tea extract showed a lower score than Example 1 in the moisturizing effect, soothing effect, anti-scavenger effect, and feeling of use, and in particular, showed unsatisfactory results in moisturizing effect and skin soothing effect. It was confirmed.

Example 1 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Moisturizing effect 4.2 3.1 3.8 3.8 3.6 Soothing effect 4.2 3.5 3.6 3.8 3.6 Rancidity reduction effect 4.1 3.5 3.8 3.1 4.1 Feeling 4.3 2.5 3.9 4.3 4.2

7 is a graph showing the results of measuring the surface tension of the fermented glycolipids prepared in Preparation Examples 1 to 3 of the present invention. Through the results of FIG. 7, all of the fermentation glycolipids of Preparation Examples 1 to 3 were excellent in interfacial activity to lower the surface tension as the concentration is increased, and particularly in the case of Preparation Example 1, which is a long fermentation glycolipid of canola oil, has excellent surface activity. Able to know.

8 is a graph showing the results of measuring the degree of emulsification (%) at 0, 30, and 60 minutes for the fermented glycolipids prepared in Preparation Examples 1-2. As a control, tween 20, a synthetic surfactant, was used. From the results of Figure 8 it can be seen that the fermentation glycolipid of 1 to 2 to produce a similar degree of emulsification capacity or emulsification stability (degree of emulsification) as a control derived from microorganisms and synthetic surfactants.

Fermented glycolipids prepared in Preparation Examples 1 to 5 of the present invention through the results of Tables 3 to 4 and FIGS. 1 to 8 may implement excellent emulsifying power, antimicrobial activity, surfactant activity, emulsion stability, and antioxidant activity. It was confirmed that the cosmetic composition comprising the artificial sterilization-preservatives, while the use of synthetic surfactants, while implementing a remarkably excellent antimicrobial activity, surfactant activity, emulsion stability and antioxidant activity.

Simple modifications and variations of the present invention can be easily made by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.

Claims (10)

A cosmetic composition comprising fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil, tocopheryl acetate, aqueous arginine solution, moisturizer, green tea extract and solvent,
The cosmetic composition is 0.1% to 10% by weight of fermented glycolipid, 0.01% to 1% by weight of ceramide, 0.005% to 0.5% by weight of phytosphingosine, 0.1% to 30% by weight of camellia seed oil, sunflower seed oil 0.1% to 10% by weight, 0.01% to 0.8% by weight of tocopheryl acetate, 0.1% to 3% by weight aqueous alginine solution, 0.1% to 30% by weight moisturizer, 0.1% to 5% by weight green tea extract Cosmetic composition containing a negative solvent.
delete The method of claim 1,
The fermented glycolipid is a cosmetic composition that is obtained by culturing Candida bombbicola on a culture substrate comprising vegetable oil, glucose, rice bran and medium.
The method of claim 3,
The vegetable oil is a cosmetic composition comprising one or more of palm oil, palm oil and canola oil.
The method of claim 3,
Wherein the culture substrate comprises 5% to 15% by weight of vegetable oil, 5% to 15% by weight of glucose, 1% to 5% by weight of rice bran, and 65% to 80% by weight of medium.
After dissolving the fermented glycolipid, ceramide, phytosphingosine, camellia seed oil, sunflower seed oil and tocopheryl acetate in the solvent, an aqueous solution of arginine, a warm dissolved moisturizer and a green tea extract are added and formulated by high speed stirring. It is a cosmetic composition manufacturing method comprising the step,
The cosmetic composition is 0.1% to 10% by weight of fermented glycolipid, 0.01% to 1% by weight of ceramide, 0.005% to 0.5% by weight of phytosphingosine, 0.1% to 30% by weight of camellia seed oil, sunflower seed oil 0.1% to 10% by weight, 0.01% to 0.8% by weight of tocopheryl acetate, 0.1% to 3% by weight aqueous alginine solution, 0.1% to 30% by weight moisturizer, 0.1% to 5% by weight green tea extract Cosmetic composition manufacturing method containing a negative solvent.
delete The method of claim 6,
The method of preparing the cosmetic composition further comprises the step of culturing Candida bombbicola on a culture substrate comprising vegetable oil, glucose, rice bran and medium to prepare a culture solution, and then removing the culture solution from impurities and extracting ethanol to obtain fermented glycolipids. Cosmetic composition manufacturing method comprising.
The method of claim 8,
The impurity removal is a method for producing a cosmetic composition comprising the removal of the cell precipitate centrifugally separating the culture, the removal of residual oil extracted hexane and the permeation of activated carbon and celite.
The method of claim 8,
Wherein the culture substrate comprises 5% to 15% by weight of vegetable oil, 5% to 15% by weight of glucose, 1% to 5% by weight of rice bran, and 65% to 80% by weight of medium.

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