CN115475126B

CN115475126B - Composition with oil control and relieving effects and application thereof

Info

Publication number: CN115475126B
Application number: CN202211153460.9A
Authority: CN
Inventors: 陈殿松; 马铃; 王靖; 常宽; 杨井国
Original assignee: Guangzhou Gude Personal Nursing Products Co ltd
Current assignee: Guangzhou Gude Personal Nursing Products Co ltd
Priority date: 2022-09-21
Filing date: 2022-09-21
Publication date: 2023-11-28
Anticipated expiration: 2042-09-21
Also published as: CN115475126A

Abstract

The application provides a composition with oil control and relieving effects and application thereof, wherein the composition with oil control and relieving effects consists of camellia seed extract and glycolipid, and the mass ratio of the camellia seed extract to the glycolipid is 1:4-4:1. The composition can be used in oil control and relieving function cosmetics, especially cosmetics requiring oil control and relieving effects such as shampoo, hair care products, scalp care products and the like, and has good application prospect.

Description

Composition with oil control and relieving effects and application thereof

Technical Field

The application belongs to the technical field of cosmetics, and particularly relates to a composition with oil control and relieving effects and application thereof.

Background

The sebum is emulsified to form a sebum film to protect the health of the scalp, but when the sebum secretion is too vigorous, a rich nutrition source is provided for the colonization of microorganisms, the sebum overflow is the hypersecretion of the sebum caused by the hypersecretion of the sebaceous glands, the hypersecretion is mainly manifested by the excessive fat and greasy hair and the increase of the filth, and if the head is not cleaned in time, external pollutants and fine dust can adhere to the scalp and the hair, and the scalp is further stimulated, so that the further secretion of the sebum of the head is increased. Often accompanied with the problems of dandruff, itching and the like, and even the seborrheic alopecia is developed for a long time, and the life quality of modern people is seriously influenced.

In order to solve the problem, the development of a composition with the effects of controlling oil and relieving can effectively inhibit sebum secretion and inflammatory factors is of great significance to the application of oil-controlling and relieving cosmetics.

Disclosure of Invention

This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.

As one of the technical schemes, the application discloses a composition with oil control and relieving effects, wherein: the composition with the oil control and relieving effects consists of camellia seed extract and glycolipid, wherein the mass ratio of the camellia seed extract to the glycolipid is 1:4-4:1.

As a preferable scheme of the composition with the oil control and relieving effects, the application is as follows: the glycolipid is sophorolipid.

As a preferable scheme of the composition with the oil control and relieving effects, the application is as follows: the camellia seed extract is a water extraction product of camellia seeds, and the preparation method of the camellia seed extract comprises the following steps: adding oil tea seed into water, heating, filtering, and drying to obtain oil tea seed extract.

As a preferable scheme of the composition with the oil control and relieving effects, the application is as follows: the sophorolipids include lactone type sophorolipids.

As a preferable scheme of the composition with the oil control and relieving effects, the application is as follows: the preparation method of the lactone type sophorolipid comprises the following steps: fermenting candida in a re-fermentation culture medium, standing for layering after the fermentation is finished, and collecting the bottom lactone type sophorolipids.

As a preferable scheme of the composition with the oil control and relieving effects, the application is as follows: further comprising extracting the collected bottom layer lactone type sophorolipids.

As a preferable scheme of the composition with the oil control and relieving effects, the application is as follows: the extraction includes extraction with ethanol.

The application also provides application of the composition in cosmetics with oil control and relieving effects. The cosmetic comprises one or more of shampoo, hair care product and scalp care product.

The application has the beneficial effects that: according to the application, the influence of the camellia seed extract and the sophorolipid on sebum secretion and inflammatory factors is researched, and experimental results show that the camellia seed extract and the sophorolipid can inhibit sebum secretion and inhibit the expression of inflammatory factors, and the camellia seed extract and the sophorolipid are found to have synergistic effect on inhibiting sebum secretion and the expression of inflammatory factors, and have strong synergistic effect when the mass ratio is 1:1, and have synergistic effect on inhibiting sebum secretion and inhibiting inflammatory factors when the mass ratio is 4:1-1:4. The application discovers that the camellia seed extract and the sophorolipid composition can be used in oil control and soothing cosmetics, especially in cosmetics requiring oil control and soothing effects such as shampoo, hair care products, scalp care products and the like, and has good application prospect.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly described below, in which:

FIG. 1 is data statistics of Nile red staining for detecting neutral lipid synthesis in SZ95 cells.

FIG. 2 is a fluorescence microscopy image of Nile red staining for detection of sebum secretion from SZ95 cells for each of the experimental groups.

FIG. 3 shows the inhibition of the IL-6 secretion level of RAW264.7 cells by each experimental group.

Detailed Description

In order that the above-recited objects, features and advantages of the present application will become more apparent, a more particular description of the application will be rendered by reference to specific embodiments thereof.

Example 1:

the anti-drop composition consists of a raw material 1 and a raw material 2, and comprises the following specific components:

raw material 1: INCI name: tea seed extract;

examples of the preparation method of the camellia seed extract include: crushing camellia seed cakes, extracting by taking water as a solvent according to the feed-liquid ratio of 1:5, wherein the extraction temperature is 80 ℃, the extraction time is 4 hours, after the extraction is finished, filtering the feed liquid to remove plant slag, ultrafiltering by using ceramic membrane equipment, enabling filtrate to pass through a 200nm ceramic membrane and then pass through a 50nm ceramic membrane to obtain clear solution, and finally spray-drying the solution to obtain a powder sample.

Raw material 2: INCI name: glycolipid

The glycolipid used in the application is sophorolipid.

The sophorolipid is a microbial secondary metabolite produced by candida through fermentation technology under certain conditions by taking sugar, vegetable oil and the like as carbon sources.

Examples of the preparation method of sophorolipids:

the strain cultivation and fermentation method comprises the following steps: YPD solid Medium (g/L): yeast powder 10, peptone 20, glucose 20, agar 20, and autoclaved at 115 ℃ for 35min.

Fermentation medium (g/L): glucose 100, yeast powder 5, KH2PO4 1, mgSO4. 7H2O 0.5,NaCl 0.1,CaCl2.2H2O 0.1, peptone 0.7, and initial rapeseed oil 50, and autoclaved at 121℃for 20min.

(1) Plate/slant culture: activating strain, picking candida strain (ATCC 22214) on YPD solid culture medium, plate streaking, activating/test tube inclined surface, culturing at 30 ℃ for 48 hours, and obtaining single colony.

(2) Seed liquid culture: single colonies were picked from the plates and inoculated into 50mL of YPD liquid medium at 30℃and 220rpm shaking for 48h.

(3) 5L fermentation tank fed-batch fermentation: the liquid amount of the 5L fermentation tank is 2L, the rotating speed is controlled to be 350-450rpm, the temperature is maintained at 30 ℃, and the ventilation is maintained at 3-6L/min. After 24h before fermentation, naOh was used to maintain the pH between 3.5 and 3.8. Samples were taken at intervals after inoculation, and the cell concentration, sophorolipid concentration and residual sugar concentration were measured, and the pH and dissolved oxygen could be read directly by electrode readings on the fermenter. Feeding with 80% (w/v) aqueous glucose solution to 20g/L or above at glucose content below 20 g/L; when the content of the rapeseed oil is lower than 30g/L, the rapeseed oil is fed to 30g/L or more.

(4) Layering collection of sophorolipids: after fermentation, the fermentation liquid is put into a separating funnel, and is settled and layered naturally through standing overnight to obtain the upper layer residual oil (recyclable), the middle layer acid type sophorolipid-based thallus fermentation liquid and the bottom layer lactone type sophorolipid-based thallus fermentation liquid.

(5) Ethanol extraction removes water-soluble macromolecular impurities: separating and collecting brown yellow oily viscous lactone type sophorolipid, adding three times of absolute ethanol, mixing, stirring for 30 min to separate flocculent proteins and residual sugar, centrifuging at 8000rpm for ten min to remove flocculent impurities, and obtaining upper clear brown yellow glycolipid ethanol solution.

(6) Spin-steaming to remove ethanol: steaming glycolipid ethanol solution at 65deg.C, removing solvent ethanol to obtain viscous clear brown lactone type sophorolipid.

The experimental material comprises the following components:

commercial SZ95 cell lines; commercial fetal bovine serum; DMEM high sugar medium, a mixture of green streptomycin and trypsin, purchased from Hyclone company in the united states; tetramethylazosin (MTT, mass fraction 98%), dimethyl sulfoxide (DMSO); IL-6 enzyme-linked immunosorbent assay (ELISA) detection kit, shanghai Biyun biotechnology Co., ltd.

The experimental method comprises the following steps:

SZ95 cell culture: SZ95 cells were cultured by conventional methods, and cells in logarithmic growth phase were taken for subsequent experiments.

MTT assay for measuring SZ95 cell viability: SZ95 cells in logarithmic growth phase were digested and counted, and the cell suspension was adjusted to 1X 104 cells/mL, and 100. Mu.L per well was inoculated into 96-well plates. The next day the medium in the well plate was aspirated and washed 1 time with PBS. And (3) adding oil tea seed extracts with different concentrations dissolved in a culture medium into sample holes, taking the extract-free extract as a control group, taking the cell-free extract and the extract as an withering group, setting 3 compound holes in each group, and continuously culturing for 24 hours. During detection, the supernatant is discarded, and a freshly prepared MTT working solution is added for light-shielding culture at 37 ℃. After 4 hours, the supernatant is discarded, 100 mu L of dimethyl sulfoxide is added into each hole, shake dissolution crystallization is carried out for 5 minutes, and OD values of each group of three compound holes are read at 490nm of an enzyme labeling instrument. The cell viability calculation formula is:

wherein, the littering hole is solvent control group.

Nile red staining method for detecting neutral lipids in SZ95 cells: SZ95 cells in the logarithmic growth phase were digested and counted, and the cell suspension was adjusted to 1X 104 cells/mL and transferred to each sample well of a black matrix-type 96-well plate in an amount of 100. Mu.L per well. The next day the medium in the well plate was aspirated and washed 1 time with PBS. Then, oil tea seed extracts or glycolipids or compositions with different purities and concentrations are added as experimental groups, and groups without active components are used as blank control groups. After 48h incubation, DMEM complete medium was discarded, carefully rinsed 2 times with PBS, 10. Mu.g/mL nile red was added and allowed to stand in the dark at 37℃for 15min. And (3) testing 485nm excitation wavelength and 565nm absorption wavelength by using a multifunctional enzyme-labeled instrument with a fluorescence function, and detecting the released fluorescence value, wherein each group comprises three complex holes.

RAW materials for RAW264.7 cytotoxicity assay: cells in log phase were collected, cell density was adjusted to 5X 104 cells/mL with DMEM, and cell suspension was added at 100. Mu.L per well in 96-well cell culture plates. Culturing in an incubator overnight. After cell attachment, old culture solution was washed away, and the sample set was added with 1. Mu.g/mL, 3. Mu.g/mL, 6. Mu.g/mL, 10. Mu.g/mL, 12.5. Mu.g/mL, 20. Mu.g/mL, 25. Mu.g/mL, 50. Mu.g/mL, 100. Mu.g/mL, and a combination solution thereof, each at a concentration of 4 wells. A normal cell group (cells and culture broth) was also set as a control group and 1. Mu.g/mL LPS group. The 96-well plate was placed in an incubator for 24 hours. 100 mu L of 0.5mg/mL MTT solution is added to each well, the culture is stopped after the culture in an incubator for 4 hours, the supernatant is carefully sucked off, 100 mu L of DMSO is added to each well, and the mixture is wrapped with tinfoil and shaken out of light. Cell viability was calculated by measuring the OD of each well at 490nm using an enzyme-labeled instrument.

Effect of the composition on LPS-induced RAW264.7 cell inflammation model:

to model LPS-induced RAW264.7 cell inflammation, the effect of both RAW materials and compositions on cell activity was first evaluated by MTT experiments, and it was found that the survival rate of cells was greater than 90% when both RAW materials or compositions were used at less than 10 μg/mL, indicating that both RAW materials and compositions had no toxic effect on cells at the concentrations of the experiment.

Effect of the raw materials and compositions on the release of the cytokine IL-6: RAW264.7 cells in logarithmic growth phase were collected, and the density of the cells was adjusted to 1X 10 with DMEM ⁵ 500 mu L of cell suspension is added into each hole of a 24-hole cell culture plate, and the experimental groups are LPS group, camellia seed extract group, sophorolipid group and composition group, and the culture is carried out for 24 hours. The supernatant was carefully aspirated. LPS group was added with 500. Mu.L of DMEM solution containing LPS (final concentration 1. Mu.g/mL); raw material to be tested + LPS group: pretreating with DMEM solutions of different concentrations of raw materials or compositions to be tested for 2 hours, adding LPS to make the final concentration 1 mug/mL, incubating together, and placing into an incubator to continue culturing for 24 hours. The OD was measured and IL-6 levels were calculated using a microplate reader by repeating 8 wells per sample according to the IL-6 kit procedure.

Experimental results:

the inhibition of sebum secretion by the composition was examined using the nile red staining method described above:

raw material 1 and raw material 2 were mixed in the proportions shown in table 1.

TABLE 1

Experiment 1 is a camellia seed extract group, and the concentration of camellia seed extracts is 1 mug/mL, 2 mug/mL, 4 mug/mL and 8 mug/mL respectively; experiment 2 was a glycolipid group, and the concentration of each glycolipid was 1. Mu.g/mL, 2. Mu.g/mL, 4. Mu.g/mL, 8. Mu.g/mL; experiment 3 shows that the oil tea seed extract and the glycolipid are mixed according to the mass ratio of 1:1, and the total concentration of the oil tea seed extract and the glycolipid mixture is 1 mug/mL, 2 mug/mL, 4 mug/mL and 8 mug/mL respectively; experiment 4 is that the camellia seed extract and the glycolipid are mixed according to the mass ratio of 4:1, and the total concentration of the camellia seed extract and the glycolipid mixture is 1 mug/mL, 2 mug/mL, 4 mug/mL and 8 mug/mL respectively; experiment 5 is that the camellia seed extract and the glycolipid are mixed according to the mass ratio of 1:4, and the total concentration of the mixture of the camellia seed extract and the glycolipid is 1 mug/mL, 2 mug/mL, 4 mug/mL and 8 mug/mL respectively.

The above nile red staining method is adopted to detect the synthesis condition of neutral lipid in SZ95 cells, the lipid synthesis level of a blank control group is set to be 1, normalization treatment is carried out on the data, and CompuSyn software is adopted to calculate CI values, wherein the CI values are 0.1-0.3 and have strong synergistic effect, 0.3-0.7 have synergistic effect, and the statistical analysis results are shown in Table 2.

TABLE 2

As shown in table 2 and fig. 1, compared with the blank control group, the camellia seed extract and the glycolipid both have the inhibition effect on sebum secretion, and the combination of the camellia seed extract and the glycolipid can synergistically inhibit sebum secretion, wherein the synergistic effect is strongest when the mass ratio of the camellia seed extract to the glycolipid is 1:1. FIG. 2 is an immunofluorescence of blank, camellia seed extract, glycolipid, camellia seed extract: glycolipid=1:1 composition group at a raw material concentration of 4 μg/mL.

The effect of each experimental group on inflammatory factor IL-6 secretion levels was examined as described above:

raw material 1 and raw material 2 were compounded and mixed as experimental groups 1 to 5, respectively, in the proportions shown in table 1. Experiment 1 is a camellia seed extract group, and the concentration of camellia seed extracts is 0.5 mug/mL, 1 mug/mL, 2 mug/mL and 4 mug/mL respectively; experiment 2 was a glycolipid group, and the concentration of glycolipids was 0.5. Mu.g/mL, 1. Mu.g/mL, 2. Mu.g/mL, and 4. Mu.g/mL, respectively; experiment 3 is that the camellia seed extract and the glycolipid are mixed according to the mass ratio of 1:1, and the total concentration of the camellia seed extract and the glycolipid mixture is 0.5 mug/mL, 1 mug/mL, 2 mug/mL and 4 mug/mL respectively; experiment 4 is that the camellia seed extract and the glycolipid are mixed according to the mass ratio of 4:1, and the total concentration of the camellia seed extract and the glycolipid mixture is 0.5 mug/mL, 1 mug/mL, 2 mug/mL and 4 mug/mL respectively; experiment 5 is that the camellia seed extract and the glycolipid are mixed according to the mass ratio of 1:4, and the total concentration of the mixture of the camellia seed extract and the glycolipid is 0.5 mug/mL, 1 mug/mL, 2 mug/mL and 4 mug/mL respectively.

Normalization was performed with 1 inflammatory factor level of macrophages under LPS stimulation. And the CI value is calculated by CompuSyn software, the CI value is 0.1-0.3, the CI value is 0.3-0.7, the CI value has synergistic effect, and the statistical analysis result is shown in Table 3.

TABLE 3 Table 3

As shown in table 3 and fig. 3, compared with the LPS group, the expression level of inflammatory factor IL-6 is obviously reduced after the camellia seed extract or the glycolipid is added, and the expression level of IL-6 of RAW264.7 cells under the stimulation of LPS can be obviously reduced after the camellia seed extract and the glycolipid are compounded, so that the camellia seed extract and the glycolipid have a synergistic effect on reducing IL-6.

According to the application, the influence of the camellia seed extract and the sophorolipid on sebum secretion and inflammatory factors is researched, and experimental results show that the camellia seed extract and the sophorolipid can inhibit sebum secretion and inhibit the expression of inflammatory factors, and the camellia seed extract and the sophorolipid are found to have synergistic effect on inhibiting sebum secretion and the expression of inflammatory factors, and have strong synergistic effect when the mass ratio is 1:1, and have synergistic effect on inhibiting sebum secretion and inhibiting inflammatory factors when the mass ratio is 4:1-1:4. The camellia seed extract and the sophorolipid composition can be used in oil control and soothing cosmetics, especially cosmetics requiring oil control and soothing effects such as shampoo, hair care products and scalp care products, and have good application prospects.

It should be noted that the above embodiments are only for illustrating the technical solution of the present application and not for limiting the same, and although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present application may be modified or substituted without departing from the spirit and scope of the technical solution of the present application, which is intended to be covered in the scope of the claims of the present application.

Claims

1. A composition with oil control and relieving effects is characterized in that: the composition with the oil control and relieving effects comprises camellia seed extract and glycolipid, wherein the mass ratio of the camellia seed extract to the glycolipid is 1:4~4:1, a step of;

the glycolipid is sophorolipid; the sophorolipids include lactone type sophorolipids;

the camellia seed extract is a water extraction product of camellia seeds, and the preparation method of the camellia seed extract comprises the following steps: adding camellia seeds into water, wherein the feed-liquid ratio is 1: heating, filtering and drying to obtain the camellia seed extract; the heating is carried out at 80 ℃; the time was 4 hours.

2. The composition with oil control and soothing effects according to claim 1, characterized in that: the preparation method of the lactone type sophorolipid comprises the following steps: fermenting candida in a fermentation culture medium, standing for layering after the fermentation is finished, and collecting the bottom lactone type sophorolipid.

3. The composition with oil control and soothing effects according to claim 2, characterized in that: further comprising extracting the collected bottom layer lactone type sophorolipids.

4. A composition having both oil control and soothing effects according to claim 3, characterized in that: the extraction includes extraction with ethanol.

5. Use of the composition of claim 1 for the preparation of a cosmetic product with oil control and soothing efficacy.

6. The use according to claim 5, characterized in that: the cosmetic comprises one or more of shampoo, hair care product and scalp care product.