KR20170143053A - Cosmetic composition for improving anti-oxidation, anti-wrinkle and moisturizing comprising fermented product of used water from washing rice and preparation method of the same - Google Patents

Abstract

The present invention relates to a functional cosmetic composition for anti-oxidation, anti-wrinkle and moisturizing improvement comprising a fermented product of used water from washing rice as an active ingredient, and a preparation method thereof. As anti-oxidation, anti-wrinkle and moisturizing improvement effects before and after fermenting used water from washing rice are compared and confirmed, provided is a functional cosmetic composition comprising a fermented product of used water from washing rice as an active ingredient.

Description

TECHNICAL FIELD The present invention relates to an antioxidant, anti-wrinkle and moisturizing functional cosmetic composition containing a rice flour fermentation culture as an active ingredient and a method for producing the same. method of the same}

The present invention relates to an antioxidant, anti-wrinkle and moisturizing cosmetic composition, and more particularly, to an antioxidant, anti-wrinkle and moisturizing functional cosmetic composition containing rice flour as an active ingredient.

In recent years, rice processed foods such as instant rice or washed rice have increased not only in ordinary households, but also as a by-product, a large amount of rice flour has been generated. Based on domestic rice consumption, the amount of water used to wash rice is about 85 million tons per year. When rice is washed for the first time, the pollution degree of rice rice reaches 6,300ppm of biological oxygen demand and about 20 billion won is consumed . When rice soils mass produced in large quantities are discharged without going through the purification process, they cause eutrophication such as rivers, lakes and coastal waters, resulting in extreme environmental pollution.

However, rice flour contains protein, fat, carbohydrates such as starch, vitamins B1, B2, and fiber. In addition, there are dozens of nutrients that are beneficial to the human body. Therefore, if rice can be recycled as a cosmetic or food material, it will be a very practical resource to reduce water pollution as well as create added value in abandoned resources.

As a prior art related to the present invention, Korean Patent Registration No. 10-1318950 discloses a method for preparing a skin whitening composition using rice soot. However, There are no known anti-wrinkle and moisturizing functional cosmetic compositions.

Accordingly, an object of the present invention is to provide an antioxidant, anti-wrinkle and moisturizing functional cosmetic composition containing rice flour as an active ingredient.

It is another object of the present invention to provide a method for producing the rice flour fermentation culture.

The above object of the present invention is achieved by immersing 10 to 40% by weight of rice as a whole in water and stirring at 100 to 150 rpm for 1 to 3 hours at room temperature to obtain only a supernatant; Sterilizing the supernatant of the above step at 121 占 폚 under high pressure for 20 minutes to confirm the hydrogen ion concentration of pH 6 to 8 and the solid content of 0.1 to 3.0;

A step of inoculating 2 to 5% of yeast such as Callactomyces or Saccharomyces with the rice sole of the above step; Fermenting the inoculum with the liquid at a temperature of 37 ° C for 1 to 4 days at 100 to 300 rpm while stirring; Anti-wrinkle and moisturizing functional cosmetic composition containing the rice flour fermentation culture obtained by filtration after autoclaving the fermented product at 90 to 121 ° C for 5 to 20 minutes as an active ingredient.

INDUSTRIAL APPLICABILITY The cosmetic composition containing the rice flour fermentation culture of the present invention as an effective ingredient has an excellent effect on antioxidation, anti wrinkle and moisturizing improvement.

1 is a photograph showing physical changes such as color and texture improvement before and after fermentation of rice flour prepared according to the present invention.
2 is a photographic view showing an image of decomposition of starch particles before and after fermentation of rice flour prepared according to the present invention.
FIG. 3 is a graph showing the results of antioxidative activity by DPPH scavenging activity of rice sole fermentation culture prepared according to the present invention.
FIG. 4 is a graph showing the results of intracellular antioxidant activity by DCF conversion of a rice flour fermentation culture prepared according to the present invention.
FIG. 5 is a graph showing the anti-wrinkle effect by measuring the type I procollagen biosynthetic amount of a rice sole fermentation culture prepared according to the present invention.
FIG. 6 is a graph showing the skin moisturizing effect of the rice flour fermentation culture produced according to the present invention.
FIG. 7 is a graph showing the effect of improving the skin density of a rice flour fermentation culture prepared according to the present invention.

The present invention provides a cosmetic composition for antioxidant, anti-wrinkle and moisturizing effect containing rice soot as an active ingredient.

According to the present invention, the rice bran of the present invention was optimized for the growth of bacteria without adding a substrate required for yeast growth.

According to the present invention, preferred yeast strains usable in the present invention may be Galactomyces sp. Or Saccharomyces sp. Strains.

For example, the strain of the genus Galactomyces is G. candidum , G. geotrichum And G. reessii And Saccharomyces sp . Are S. cerevisiae , S. dairenensis And S. ellipsoideus are preferred.

In addition, the cosmetic composition containing the rice flour fermentation culture produced according to the present invention as an active ingredient can be manufactured into any formulation conventionally produced in the art, and can be formulated into, for example, lotion, cream, can do.

The present invention will be described in more detail with reference to the following specific examples, experimental examples and preparation examples. However, the following examples, experimental examples and preparation examples are for illustrative purposes only and are not intended to limit the scope of the present invention. It does not.

Example 1. Preparation of rice flour preparations of the present invention

The supernatant obtained by immersing 10 to 40% by weight of rice in water at 100 to 150 rpm for 1 to 3 hours at 25 DEG C was sterilized at 121 DEG C for 20 minutes under high pressure to give a pH of 6 to 8, A rice flour having a solid content of 0.1 to 3.0 was obtained.

Example 2. Production of fermentation culture of yeast strain using rice soap preparation

In order to prepare a fermentation culture using the crude rice preparation obtained in Example 1 according to the present invention, a rice broth prepared by sterilizing with a 2-5 L fermenter was added to Galactomyces or Saccharomyces Saccharomyces sp. Strain was inoculated with 2 ~ 5% of the strain, and the mixture was subjected to liquid fermentation by stirring at 25 to 40 캜, most preferably at 30 캜 for 1 to 4 days at 100 to 300 rpm, most preferably 200 rpm. When the cultivation of the yeast strains was completed, the strains were sterilized by pressurization at 90 to 121 ° C for 5 to 20 minutes and then filtered to obtain a rice flour fermentation culture of the present invention.

As can be seen from FIGS. 1 and 2, the rice flour fermentation culture of the present invention was able to improve color and sedimentation problems unsuitable for use as raw materials for cosmetic raw materials.

Experimental Example 1 Antioxidant activity by DPPH scavenging ability

The antioxidant activity of the rice fermented culture obtained in Example 2 of the present invention was measured by using 1,1-diphenyl-2picrylhydrazyl radical (DPPH). DPPH exists as a radical of stabilized structure of the nitrogen center in the compound, and the antioxidative activity can be measured according to the degree of scavenging of the stabilized radical.

In order to evaluate this, 100 .mu.l of 0.2 mM DPPH and 100 .mu.L of the test compound were mixed with the rice flour fermentation culture obtained in Example 2 of the present invention, and reacted at room temperature for 20 minutes. Then, the absorbance was measured at 517 nm using a microplate reader.

As shown in FIG. 3, the antioxidative activity by DPPH scavenging activity was increased by DPPH scavenging activity as the concentration was increased.

Experimental Example 2 Intracellular antioxidant activity by DCF conversion

The antioxidant activity of the rice flour fermentation culture obtained in Example 2 of the present invention was confirmed by flurimetric assay using DCFH-DA (2 ', 7'-dichlorofluorescin-diacetate). In the fluorimetric assay, the DCFH-DA can easily enter the cell as a nonpolar molecule. When the DCFH-DA is separated by the intracellular esterase into the nonpolar polar molecule DCFH and oxidized by the active oxygen in the cell, the fluorescent DCF (2 ', 7'- dichlorofluorescin, which is measured by a fluorometer.

In order to evaluate this, dermal fibroblasts (HDFn) were cultured in DMEM (supplemented with 10% FBS and 1% antibiotic) at 37 ° C in a 5% CO ₂ incubator and cells growing in the larval stage were used in the experiment. Wells were inoculated in 96 wells at a rate of about 1 x 10 ⁴ cells per well. Then, the rice fermented culture of Example 2 was treated at 0.1, 0.5, and 1% (v / v) And cultured in a 5% CO ₂ incubator for 1 hour. Then, 1 mM of H ₂ O _{2 as an} oxidative stress inducer was added, 20 μM of DCFH-DA was added, and the fluorescence reaction was analyzed by a fluorescence spectrophotometer (excitation 485 nm, emission 538 nm) at 37 ° C. for 90 minutes. As a control, untreated rice without the rice flour fermentation culture of the present invention was used, and the active oxygen species inhibiting ability was calculated by calculating the fluorescence intensity of the experimental group as compared with the control group using the following formula (1). All experiments were repeated 3 times.

As a result, as shown in FIG. 4, the inhibition rate of reactive oxygen species was higher in the group treated with rice flour fermentation according to the present invention than the control group. In particular, when the rice flour fermentation broth of the present invention was treated with 1% (v / v), high oxygen species inhibition rate was exhibited.

Experimental Example 3. type I procollagen biosynthetic amount measurement

In order to confirm the cell proliferation and anti-wrinkling effect of the rice flour fermentation culture prepared according to the present invention of Example 2, in order to measure the type I procollagen biosynthesis amount of the fibroblasts in the rice flour fermentation culture of the present invention, procollagen type I C -peptide ELISA kit (TaKaRa, MK101) was used.

For this purpose, fibroblasts were inoculated on a 48-well plate at a concentration of 5 × 10 ⁴ cells / mL and then proliferated. The medium used was DMEM medium containing 10% serum. Twenty-four hours after inoculation, the test samples were added to the medium at 0.1, 0.5 and 1%, and cultured in a 5% CO ₂ incubator at 37 ° C for 1 day. The medium was transferred to a tube and centrifuged at 15,000 rpm for 30 sec. The supernatant was used as a test sample and procollagen type I C-peptide was measured. As a control group, rice flour before fermentation was used instead of the rice flour fermentation culture of the present invention.

As a result, as shown in FIG. 5, the amount of type I procollagen biosynthesis was increased in the experimental group to which 0.1, 0.5 and 1% of the rice flour fermentation broth of the present invention was added, respectively, Anti-wrinkle effect was confirmed.

Experimental Example 4. Skin moisture content measurement

In order to measure the moisture content of the rice flour fermentation culture obtained in Example 2 of the present invention, an experiment was conducted using the electric capacity.

For this purpose, a healthy subject (n = 8, 28-42 years old) was selected and applied continuously for 2 days to the 2cm x 2cm area for 15 days, and the corneoometer (pin probe type, DermaScan, Cortex technology, Denmark) The dose was measured. As a control, the same method was applied for the same period of time using a solvent or a water-free form containing no fermentation culture.

As a result, as shown in FIG. 6, in the group to which the rice flour fermentation culture of the present invention was applied, significant moisturizing effect was observed at 4 days, 7 days and 12 days after the application. As a result of the experiment of the present invention, the total number of subjects did not show a stimulus response to the rice flour fermentation culture.

Experimental Example 5 Measurement of skin density improvement

Ultrasonic wave was used to measure the skin density improving effect of the rice flour fermentation culture obtained in Example 2 of the present invention. In general, with aging, hormone activity is inhibited, skin density is reduced, and wrinkles are increased due to reduced skin density. The skin density and thickness of the epidermal layer and the dermal layer were measured by ultrasonography to measure the skin density.

For this purpose, healthy subjects (n = 9, 28 ~ 42 years old) were selected and applied continuously for 15 days in a 2cm x 2cm area and absorbed for 5 minutes. Using a high frequency skin ultrasound device (DermaScan, 20MHz, Cortex technology, Denmark) And the area where the rice flour fermentation culture of the present invention was absorbed was measured.

As a result, as shown in FIG. 7, a significant increase in skin density was observed at 12 and 15 days after application in the group treated with the rice flour fermentation culture of the present invention, as compared with the control group.

Hereinafter, various cosmetic formulations preferable for using the present invention are disclosed, but it goes without saying that the scope of right of the present invention is not limited to the disclosed formulations.

Production Example 1 toilet water

The skin lotion containing the rice flour fermentation culture of the present invention obtained in Example 2 of the present invention was prepared as shown in Table 1 below.

Raw material Raw material Content (% by weight) One The rice flour fermentation culture of the present invention 10.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance

Production Example 2 Lotion

The lotion containing the present rice flour fermentation culture obtained in Example 2 of the present invention was prepared as shown in Table 2 below.

Raw material Raw material Content (% by weight) One The rice flour fermentation culture of the present invention 10.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbidestalate 1.0 7 Chin type mono-stearic acid glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

Production Example 3 cream

The cream containing the rice flour fermentation culture of the present invention obtained in Example 2 of the present invention was prepared as shown in Table 3 below.

content Raw material Content (% by weight) One The rice flour fermentation culture of the present invention 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

Production Example 4 Essence manufacturing

The essence containing the rice flour fermentation culture of the present invention obtained in Example 2 of the present invention was prepared as shown in Table 4 below.

content Raw material Content (% by weight) One The rice flour fermentation culture of the present invention 10.0 2 Sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 젱 젱 Balance

INDUSTRIAL APPLICABILITY As described above, the cosmetic composition containing the rice flour fermentation culture of the present invention as an active ingredient has an excellent antioxidation, anti-wrinkle and moisturizing function, and thus is a very useful invention in the cosmetics industry.

Claims (6)

A step of inoculating yeast strains into high-pressure sterilized rice flour preparations and fermenting liquid using a fermentation tank; A step of pressurizing and sterilizing the liquid fermentation product obtained in the step (a), and then filtering the liquid fermentation product.
Wherein the yeast strain of claim 1 is one or more strains selected from the genus of the genus Lactococcus or the genus of the genus Saccharomyces.
A rice flour fermentation culture produced according to the method of claim 1.
An anti-oxidative, anti-wrinkle and moisturizing functional cosmetic composition characterized by containing the rice flour fermentation culture of claim 3 as an active ingredient.
The cosmetic composition according to claim 4, wherein the cosmetic composition comprises 0.001 to 100% (v / v) of a rice flour fermentation culture relative to the total cosmetic composition.
The cosmetic composition according to claim 4, wherein the functional cosmetic composition is any one selected from the group consisting of lotion, lotion, cream and essence.

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