KR102029599B1 - Cosmetic composition for improving skin anti-oxidation, anti-wrinkle and moisturizing comprising fermented product of used water from washing rice and preparation method of the same - Google Patents

Abstract

The present invention relates to an antioxidant, anti-wrinkle and moisturizing improvement functional cosmetic composition containing a rice water fermentation culture as an active ingredient, and a method for manufacturing the same, and to improving skin antioxidant, anti-wrinkle and moisturizing effects before and after fermentation of rice water as a cosmetic raw material. By comparing and confirming, there is an excellent effect of providing a cosmetic composition for improving skin anti-wrinkle containing fermentation broth as an active ingredient.

Description

Cosmetic composition for improving skin anti-oxidation, anti-wrinkle and moisturizing comprising fermented product of used water from washing rice and preparation method of the same}

The present invention relates to a cosmetic composition for improving anti-wrinkle and moisturizing effect through skin antioxidant, skin collagen formation and skin density improving effect. More particularly, the present invention relates to an antioxidant, anti-wrinkle and moisturizing improving functional cosmetic containing a fermentation broth as an active ingredient. It relates to a composition.

Recently, rice processed foods such as instant rice or washed rice are increasing as well as in general households, and a large amount of rice water is generated as a by-product. Based on domestic rice consumption, the amount of water used to wash rice is about 85 million tons per year, and when washing rice for the first time, the pollution level of rice water reaches 6300 ppm and biological oxygen demand is about 20 billion won. It is estimated. When the mass produced rice water is discharged without purification, it causes eutrophication of rivers, lakes and coastal waters, resulting in severe environmental pollution.

However, rice water contains protein, fat, carbohydrates, including starch, and vitamins B1, B2, and fiber. In addition, dozens of nutrients are beneficial to the human body. Therefore, if rice water can be recycled as a cosmetic or food material, it will be a very practical resource that not only reduces water pollution but also creates added value from resources that have been discarded.

Prior art related to the present invention is known in the Republic of Korea Patent No. 10-1318950, but this relates to a method for preparing skin whitening or moisturizing functional composition using rice water to date fermented rice water using a microbial strain. There is no known functional cosmetic composition containing the culture as an active ingredient.

Accordingly, an object of the present invention is to provide a skin antioxidant, anti-wrinkle and moisturizing functional cosmetic composition containing a rice water fermentation culture as an active ingredient.

Another object of the present invention is to provide a method for producing a fermentation culture of the above-mentioned anti-wrinkle rice water.

The object of the present invention is to obtain a supernatant by immersing 10 to 40% by weight of rice in water relative to the total weight in water at 100 to 150rpm for 1 to 3 hours at room temperature; Sterilizing the supernatant obtained in the above step at 121 ° C. for 20 minutes to prepare a pH of hydrogen by checking pH 6 to 8 and a solid content of 0.1 to 3.0 wt%;

Inoculating 2 to 5% of the total weight of the rice water with any yeast selected from the yeast strain of the genus Galactomyces or Saccharomyces of the rice water obtained in the step; Liquid fermentation of the yeast strain inoculum obtained in the above step with stirring at 100-300 rpm for 1-4 days at 37 ° C. temperature; Sterilization of the liquid fermentation product obtained in the above step at 90-121 ° C. for 5-20 minutes to prepare a rice water fermentation culture obtained by filtration and to improve skin antioxidant, anti-wrinkle and moisturizing containing the fermentation broth as an active ingredient Achieved by providing a functional cosmetic composition.

The cosmetic composition containing the rice water fermentation culture of the present invention as an active ingredient has an excellent effect on improving skin antioxidant, anti-wrinkle and moisturizing.

1 is a photograph showing physical changes such as color and texture improvement before and after fermentation of rice water prepared according to the present invention.
Figure 2 is a photograph showing the decomposition of the starch particles before and after the fermented rice water prepared according to the present invention.
Figure 3 is a graph showing the results of antioxidant activity by DPPH scavenging ability of the rice water fermentation culture prepared according to the present invention.
Figure 4 is a graph showing the results of intracellular antioxidant activity by the DCF conversion of rice water fermentation culture prepared according to the present invention.
Figure 5 is a graph showing the skin anti-wrinkle effect through the measurement of the type I procollagen biosynthesis amount of the rice water fermentation culture prepared according to the present invention.
Figure 6 is a graph showing the skin moisturizing effect of the rice water fermentation culture prepared according to the present invention.
Figure 7 is a graph showing the skin density improvement effect of the rice water fermentation culture prepared according to the present invention.

The present invention provides a cosmetic composition for improving skin antioxidants, anti-wrinkle and moisturizing containing rice water fermentation culture as an active ingredient.

According to the present invention, the rice water fermentation culture of the present invention optimized the growth of the yeast strain used in the present invention without adding a substrate required for the growth of the yeast strain.

The most preferred yeast strain that can be used in the present invention should be any one selected from the genus Galactomyces ( Saccharomyces) or Saccharomyces ( Saccharomyces) .

For example, in particular galacto My process in strains from yeast strain G.candidum, G.geotrichum and G. reessii and Saccharomyces strains were most preferred S. cerevisiae, S. dairenensis and S.ellipsoideus .

In addition, the cosmetic composition containing the rice water fermentation culture prepared according to the present invention as an active ingredient can be prepared in any formulation commonly prepared in the art, for example, formulated in a lotion, lotion, cream and essence, etc. can do.

The present invention will be described in more detail by the following specific examples, experimental examples and preparation examples, but the following examples, experimental examples and preparation examples are intended to illustrate the invention and the scope of the present invention is intended to be limited thereto. It doesn't work.

Example 1 Production of Rice Water Preparation of the Invention

The supernatant obtained by immersing 10 to 40% by weight of rice in water and stirring it at 100 to 150 rpm for 1 to 3 hours at 25 ° C. was autoclaved at 121 ° C. for 20 minutes under hydrogen ion concentration pH 6 to 8, A rice water pretreatment preparation having a solid content of 0.1 to 3.0% by weight was obtained.

Example 2. Production of Yeast Strain Fermentation Culture Using Rice Water Preparation

Galactomyces or Galactomyces in the pretreated rice water preparation using a 2-5L fermentation tank to produce a rice water fermentation culture using the rice water pretreatment preparation obtained in Example 1 according to the present invention or Saccharomyces ( Saccharomyces ) yeast strain 2 to 5% inoculation and liquid fermentation by stirring at 100 ~ 300rpm, most preferably at 200rpm for 1-4 days at 25 ~ 40 ℃ most preferably at 30 ℃. After the incubation of the yeast strain, the final product rice water fermentation culture of the present invention was obtained through high-pressure sterilization at 90-121 ° C. for 5 to 20 minutes.

The rice water fermentation culture of the present invention, as can be seen in Figures 1 and 2 was able to improve the problem of color and precipitation, which is not suitable for use as a raw material as a cosmetic raw material.

Experimental Example 1. Antioxidant Activity by DPPH Scavenging Activity

The antioxidant activity of the rice water fermentation culture obtained in Example 2 of the present invention was measured by using 1,1-diphenyl-2picrylhydrazyl radical (DPPH). DPPH exists as a radical with a stabilized structure of nitrogen center in the compound, and antioxidant activity can be measured according to the degree of scavenging ability of the stabilized radical.

In order to evaluate this, the rice water fermentation broth obtained in Example 2 of the present invention was used as a test compound, and 100 μL of 0.2 mM DPPH and 100 μL of the test compound were reacted at room temperature for 20 minutes, and then absorbance was measured at 517 nm using a microplate reader.

As shown in FIG. 3, the antioxidant activity of DPPH scavenging activity increased as the concentration increased.

Experimental Example 2. Intracellular Antioxidant Activity by DCF Conversion

* Antioxidative effect of the rice water fermentation culture obtained in Example 2 was confirmed by flurimetric assay using DCFH-DA (2 ', 7'-dichlorofluorescin-diacetate). The fluorimetric assay shows that when DCFH-DA can easily enter the cell as a nonpolar molecule and is separated into DCFH which is a non-fluorescent polar molecule by esterase in the cell, and then oxidized by free radicals in the cell, fluorescent DCF (2 ', 7'- dichlorofluorescin) is converted to fluorometer.

To evaluate this, skin fibroblasts (HDFn) were cultured in DMEM (10% FBS and 1% antibiotics) medium at 37 ° C., 5% CO ₂ incubator, and cells grown in log phase were used for the experiment. After inoculating 96 wells so as to have about 1 x 10 ⁴ cells per well, the fermentation broth of Example 2 of the present invention was treated with 0.1, 0.5, and 1% (v / v) concentrations, respectively, at 37 ° C., Incubated for 1 hour in a 5% CO ₂ incubator. Then, oxidative stress-inducing substance H ₂ O ₂ 1mM was added, DCFH-DA 20μM was added and fluorescence reaction was analyzed for 90 minutes at 37 ℃ by fluorescence spectrophotometer (excitation 485nm, emission 538nm). As a control group, an untreated group was not treated with the fermentation broth of the present invention, and active oxygen species inhibition was quantified by calculating the fluorescence intensity of the experimental group compared to the control group by% using the following Equation 1. All experiments were repeated three times.

Experimental results, as can be seen in Figure 4, the reactive oxygen species inhibition rate showed a high reactive oxygen species inhibition rate in the group treated with the rice water fermentation culture of the present invention compared to the control group. In particular, when treated with 1% (v / v) of the rice water fermentation culture of the present invention showed a high oxygen species inhibition rate.

Experimental Example 3. Type I procollagen biosynthesis measurement

In order to determine the cell proliferation and anti-wrinkle effect of the rice water fermentation culture prepared according to Example 2 of the present invention procollagen type I C to measure the type I procollagen biosynthesis amount of fibroblasts to the rice water fermentation culture of the present invention Peptide ELISA kit (TaKaRa, MK101) was used.

To this end, fibroblasts were grown after inoculation on a 48 well plate at a concentration of 5 × 10 ⁴ cells / mL. As the medium, DMEM medium containing 10% of serum was used. Twenty four hours after inoculation test samples were added to the medium at 0.1, 0.5 and 1% and incubated in a 37 ° C., 5% CO ₂ incubator for 1 day. Procollagen type I C-peptide was measured using the supernatant obtained by transferring the medium to the tube and centrifuging at 15,000 rpm for 30 seconds. As a control, rice water before fermentation was used instead of the present invention rice water fermentation culture.

As shown in FIG. 5, the amount of type I procollagen biosynthesis was increased in the experimental group to which 0.1, 0.5, and 1% of the rice water fermentation cultures of the present invention were added, respectively. The skin anti-wrinkle effect was confirmed.

Experimental Example 4. Skin Moisturizing Content Measurement

The present invention was experimented using the electric capacity to measure the water content of the rice water fermentation culture obtained in Example 2.

To this end, healthy subjects (n = 8, 28-42 years old) were selected and applied to the area of 2cm x 2cm for 15 days for 5 minutes and absorbed for 5 minutes, and the corneeometer (pin probe type, DermaScan, Cortex technology, Denmark) Dose was measured. As a control, it was measured by applying the same method for the same period using a solvent or a homemade form containing no fermentation culture.

Experimental results, as can be seen in Figure 6 showed a significant moisturizing effect at each time point, 4 days, 7 days and 12 days after the application in the group of the present invention the rice water fermentation culture compared to the control group. Experimental results of the present invention did not show any irritant response to the rice water fermentation culture.

Experimental Example 5. Skin Density Improvement Measurement

Ultrasonic wave was used to measure the skin density improvement effect of the rice water fermentation broth obtained in Example 2 above. In general, with aging, hormonal activity is inhibited and skin density decreases, causing wrinkles to increase due to reduced skin density. In order to measure the skin density, the skin density and thickness of the epidermal and dermal layers were measured by ultrasound diagnosis.

To this end, healthy subjects (n = 9, 28-42 years old) were selected and applied continuously for 2 days at 2cm x 2cm for 15 days and absorbed for 5 minutes, using a high frequency skin ultrasound device (DermaScan, 20MHz, Cortex technology, Denmark). The portion of the rice water fermentation culture of the present invention was measured.

As a result, as shown in Figure 7, compared to the control group showed a significant skin density increase effect at 12 and 15 days after the application in the group of the rice water fermentation culture of the present invention.

Hereinafter, various cosmetic formulations are preferred for use in the present invention, but the scope of the present invention is not limited to the disclosed formulations.

Preparation Example 1. toilet water

The present invention to prepare a lotion containing the present invention rice water fermentation culture obtained in Example 2 as shown in Table 1.

Raw material Raw material Content (% by weight) One Rice Rice Fermentation Culture 10.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene Cured Castor Oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Remaining amount

Preparation Example 2. Lotion of the Invention The lotion containing the inventive rice-water fermentation culture obtained in Example 2 was prepared as shown in Table 2 below.

Raw material Raw material Content (% by weight) One Rice Rice Fermentation Culture 10.0 2 Beeswax 1.0 3 Polysorbate 60 1.5 4 Solvidan Sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbidate stearate 1.0 7 Lipophilic Monostearine Glycerin 0.5 8 Stearic acid 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy Polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Remaining amount

Preparation Example 3. Cream of the present invention The cream containing the present invention rice water fermentation culture obtained in Example 2 was prepared as shown in Table 3 below.

content Raw material Content (% by weight) One Rice Rice Fermentation Culture 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Solvanesesquioleate 0.5 7 Glyceryl Monostearate / Glyceryl Stearate / Polyoxyethylene Stearate 1.0 8 Wax 1.0 9 Floating paraffin 4.0 10 Squalane 4.0 11 Caprylic / Capric Triglycerides 4.0 12 Carboxy Vinyl Polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Remaining amount

Preparation Example 4. Essence Preparation Essence of the present invention Rice essence fermentation culture of the present invention obtained in Example 2 was prepared as shown in Table 4.

content Raw material Content (% by weight) One Rice Rice Fermentation Culture 10.0 2 Cytostolol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steares-4 1.2 6 cholesterol 1.5 7 Dicetylphosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxy Vinyl Polymer 0.2 11 Xanthan Gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Divisor Remaining amount

As described above, the cosmetic composition comprising the present rice water fermentation culture as an active ingredient has an excellent effect of improving anti-wrinkle and moisturizing function through skin antioxidant, skin collagen formation and skin density enhancing effect. will be.

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Claims (5)

10 to 40% by weight of the total weight of the rice immersed in water and stirred at 100 to 150 rpm for 1 to 3 hours at room temperature to obtain a supernatant; Sterilizing the supernatant obtained in the above step at 121 ° C. for 20 minutes to prepare a rice water by confirming the hydrogen ion mobility PH 6 to 8 and the solid content of 0.1 to 3.0 wt%; Inoculating 2 to 5% of any yeast selected from the yeast strain of the genus Galactomyces or Saccharomyces of the rice water obtained in the step; Liquid fermentation of the yeast strain inoculum obtained in the above step while stirring at 37 ° C. at 100˜300 rpm; Method for producing a rice water fermentation culture, characterized in that the liquid fermented product obtained in the above step is obtained by high-pressure sterilization at 90 ~ 121 ℃ for 5 to 20 minutes after filtration. Rice water fermentation culture for improving skin anti-wrinkle prepared by the method of claim 1. Cosmetic composition containing a fermentation culture of the rice water for improving the anti-wrinkle of claim 2. The cosmetic composition for improving skin anti-wrinkle according to claim 3, wherein the fermentation culture of the rice water is contained in an amount of 0.001 to 100% (v / v) relative to the total cosmetic composition. The cosmetic composition for improving skin anti-wrinkle according to claim 4, wherein the cosmetic composition is any one selected from a lotion, lotion, cream and essence.

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