KR20180052823A - Cosmetic compositions and wet tissue solution containing natural preservatives - Google Patents

Abstract

The present invention relates to a natural preservative cosmetic composition comprising a gallnut extract, a pine needle extract, a mother chrysanthemum extract and a scutellaria extract, and a wet tissue solution composition containing the same. A wet tissue including the natural preservative cosmetic composition of the present invention is a natural wet tissue comprising natural ingredients, and has excellent skin stability and antimicrobial ability as compared with conventional chemical preservatives, and is excellent in detergency and moisturizing. In addition, despite the fat that a plant extract is contained, a specific color and a specific odor inherent to the plant extract are not exhibited, so that feeling of use of consumers can be enhanced.

Description

COSMETIC COMPOSITIONS AND WET TISSUE SOLUTION CONTAINING NATURAL PRESERVATIVES < RTI ID = 0.0 >

The present invention relates to a natural preservative cosmetic composition having an excellent antimicrobial activity and an antiseptic activity by mixing natural antimicrobial raw materials at an appropriate ratio and having a lower possibility of skin irritation than a chemical preservative. More specifically, it has excellent antimicrobial activity, including antioxidant extract, pine tree leaf extract, green tea extract, and golden extract. It has excellent antimicrobial spectrum and excellent antiseptic ability against cosmetics, and also has physicochemical stability and effect on cosmetic formulation And is low in the possibility of causing skin irritation due to no addition of a chemical preservative.

Conventional cosmetic compositions may be contaminated with microorganisms during manufacturing process, distribution process, or consumer use when manufacturing or distributing without adding a chemical preservative (labeled designation ingredient), which may cause skin safety problems. Indication Designated ingredient refers to ingredients that the Food and Drug Administration (KFDA) should label the ingredients and designate the permitted content as legally required if they are used with concern about harmfulness to human body. Marking Phosphate as a precursor to tar, pigments, preservatives, antimicrobials, antihistamines, sunscreens, gold leaf, and hair products.

Among them, preservative is a medicine to prevent the decay of the material, and it is a preservative to prevent the vegetable organic material from being corrupted by the action of the microorganism, and the preservative is added to preserve the material for preservation purpose. Preservatives that prevent microbial decay are essential for long-term quality of products such as food, medicines and cosmetics. Particularly, cosmetics have a relatively long distribution period and are highly likely to contact with microorganisms in their use and storage methods. Various carbon sources and nitrogen sources are blended, and moisture content necessary for microbial growth is high. There is a growing need and problem with preservative treatment.

If the proper preservative system is not established in the cosmetics, microorganisms are proliferated during the manufacturing process and the consumer use process, resulting in metabolism of the microorganisms, causing offensive odors, discoloration and product pH changes, And also has a detrimental effect on the human body such as causing stimulation to the human body due to metabolites and microorganisms themselves generated during microbial metabolism. In particular, pathogenic microorganisms cause disease.

Therefore, preservatives in cosmetics are not simply added to prevent product corruption, but they are not added from the time the cosmetics are manufactured in the factory, until the final use of the product by the consumer, Is a generic term for additives to be added for the purpose of inhibiting the growth of microorganisms contained in products or contaminated and preventing the deterioration, deterioration and deterioration of cosmetics caused by the growth of microorganisms.

Proper addition of preservatives to cosmetics not only prevents microbial contamination of the product due to microbial contamination of raw materials and manufacturing processes, but also prevents microbial contamination during consumer use and storage, maintains product characteristics, And stability, thereby minimizing health hazards to consumers.

There are many kinds of preservatives used in cosmetics, and as the production of cosmetics increases, the preservatives are becoming more diverse and their usage is also increasing. However, on the other hand, preservatives may affect the public health, and in Korea, the kind and combination limit is designated as a labeling substance by the Korea Food and Drug Administration. (Article 4, Article 3, Article 9 and Article 13, Article 6 of the Cosmetics Act, Notice No. 2000-27 of the Korea Food and Drug Administration) Most of the preservatives having such a preservative effect are artificially chemically produced, easy to manufacture, The synthesized material is predominant. Examples of such preservatives include Methyl Paraben, Ethyl Paraben, Propyl Paraben, Butyl Paraben and Quaternium-15, which are typical examples of preservatives for cosmetic preservatives. Imidazolidinyl urea diazolidinyl urea, phenoxyethanol, benzalkonium chloride, chlorohexidine gluconic acid, trichlosan urea, trichloxacin, And the like.

However, these chemical preservatives inhibit the growth of microorganisms, thereby imparting safety and stability to cosmetics, while at the same time causing irritation to the user's skin. Typical examples are formalin glass-type preservatives. Formalin glass-type preservatives, including diazolidinyl urea and imidazolidinyl urea, have recently been reviewed for safety in the world and their use is on the decline, Cleaning products and some wash off products. It is also a preservative that is prohibited in some countries, including Japan, or strictly controls its use.

These formalin free-form preservatives are known to gradually release the formalin group contained in the structure over time, and thus the liberated formalin is known to not only cause skin irritation but also cause cancer.

Even preservatives of parabens commonly used in cosmetics and medicines are safely used as conventional chemical preservatives, and skin antiseptics such as skin allergies (Andrea Counti et al., Contact Dermatitis, 1997, 37, 35-36) , Toxicology and Applied Pharmacology, 1998, 153, 12-19) and resistance to inducing resistant bacteria. In addition, there is a possibility of new problems such as rapid, chronic toxicity and mutagenesis due to persistent accumulation in the body, which is disallowed within the permitted standards (Shin Dong-hwa, Food Science and Technology, 1990, 23 (4) 68-72).

As described above, the side effects of such a chemical preservative cause the user to lower the reliability of the product, ultimately causing consumer complaints on cosmetic and beauty products.

Therefore, active research is being carried out to reduce the preservative content in cosmetics as much as possible, or to use a no-preservative system that does not use preservatives, and to solve the side effects of conventional chemical preservatives and to protect the skin from hypoallergenic And the need for natural preservatives without toxicity and resistance are emerging.

Antimicrobial activity of alkaloids, flavonoids, phytoalexins, antimicrobial peptides, and organic acids and fatty acids has been reported in studies on natural antimicrobial substances. It is presumed that this is due to the effect of chelate (EI-Shenawy, MA et al., J. Food Protec., 1989, 52 (11), 771-778) (Bizri, JN et al., J. Food Science, 1994 , 59 (1), 130-135).

Currently, there are natural antimicrobial agents that are commercialized, such as Hinokitiol, a Magnoliae extract, Megnonol, and grapefruit seed extract (DF-100), but most of them suffer from problems such as economical efficiency, antimicrobial spectrum, The need for more advanced natural antimicrobial agents.

Antimicrobial peptides have also been studied as an alternative to the combined effect (Giacometti A et al., J. Antimicrob. Chemother 2000, 46 (5), 807-811), but they are relatively weak in heat because they are physicochemical peptides, It is very vulnerable to widely available proteases and thus has limitations in general use. In addition, since they lack the selectivity for bacteria and fungi and eukaryotes due to their action mechanism, they are limited to be used as medicines. Also, since the microorganisms must be cultivated in the manufacturing process and then separated and purified, (Lee JH et al., Protein. Ex. Purif, 1998 Feb; 12 (1), 53-60).

Korean Patent No. 10-0481637 discloses a composition comprising tetraacetyl phytosphingosine, Hydrolite-5, polyglyceryl-2 laurate / glyceryl caprate / polyglyceryl-10 laurate, grapefruit seed extract, The cosmetic composition of the present invention is characterized in that the extract is added in accordance with each prescription. However, there is a possibility that irritation may be induced as a cosmetics composition with no chemical additives, except grapefruit seed extract and Cordyceps sinensis extract, and tetraacetyl phytosphingosine , There is a problem that the solubility is very low and the application to cosmetics requiring various forms of formulation is restricted.

In addition, Korean Patent Application No. 10-2004-0080717 discloses a cosmetic composition containing chitosan and grapefruit seed extract having antiseptic and antibacterial activity, but in the case of grapefruit seed extract and chitosan, Pseudomonas aeruginosa It is difficult to apply it to various types of cosmetics because of its weak antimicrobial activity against Gram-negative bacteria such as labor and management, and its application to cosmetics is limited due to strong positive charge of chitosan.

Accordingly, in order to solve the side effects of conventional chemical preservatives and to overcome the problems as raw materials for cosmetics such as natural antimicrobial agents, the present inventors have studied not only antibacterial activity against various natural plants, but also properties as raw materials for cosmetics, Pine needle leaf extract, red ginseng extract, and gold extract, when used properly, exhibit excellent antimicrobial activity against gram-negative bacteria, gram-positive bacteria, fungi including yeast and fungi, and have broad antimicrobial spectrum, The inventors of the present invention have found that the cosmetic composition of the present invention has superior physical, chemical stability and cosmetic formulation effects, has a very low possibility of skin irritation compared with conventional chemical preservatives, and develops a natural preservative cosmetic composition.

An object of the present invention is to solve the side effects of conventional chemical preservatives such as possibility of skin irritation and to overcome the problems as raw materials for cosmetics such as existing natural antibacterial agents, To provide an excellent natural preservative cosmetic composition having very little influence on physico-chemical stability and cosmetic formulation.

It is still another object of the present invention to provide a wet tissue solution composition having physicochemical stability by adding a surfactant and various humectants to a natural preservative cosmetic composition in order to use the natural preservative cosmetic composition as a wet tissue solution.

In order to achieve the above object, the first aspect of the present invention provides a natural preservative cosmetic composition comprising a rhododendron extract, a pine leaf extract, a rhododendron extract and a gold extract.

Hereinafter, the present invention will be described in detail.

The cosmetic composition of the present invention is a composition obtained by mixing a rhododendron extract, a pine needle leaf extract, a rhododendron extract and a gold extract in an appropriate ratio, exhibiting an excellent antimicrobial activity, exhibiting excellent antimicrobial spectrum and excellent antiseptic properties against cosmetics, And is a natural preservative cosmetic composition to which a chemical preservative is not added.

The Rhus japonica L. of the present invention refers to an insect house created by giving aphid (Aphis chinensis J. Bell) to a leaf of Rhus japonica L. (Rhus japonica L., Anacardiaceae) belonging to the Rhus verniciflua family, Distributed. The process of creating a house of insects is as follows. Late September An insect worm with a wing through a hole in an insect house lays a cub insect on the middle host, Minumver sicatum (M. trichomane). The puppy sucks the juice of the cetacean, grows it, makes a house of insects with white pellets, and goes to winter. It becomes a pupa in the spring of the following year, and comes out of the pupa in late April and becomes a winged insect. The female worms give birth to wingless worms on the branches of the rhusi after mating and die. Wingless worms migrate to young leaves and grow into insects at this time. At that time, the size is five times the original size, so it is said that it is called obi. Before the early autumn bug leaves, it picks up the insect house, steam it in the steam, kills the insect, and dries. The outer surface is grayish brown with soft hairs, 3 ~ 7cm in length, 2 ~ 5cm in width, 2mm in thickness, hard and easily broken. The genus is usually empty or gray-white dead worms and secretions remain, and there is a disgusting smell. The collected dwarf is dried, boiled or steamed in the sun. The main component is pyrogallol tannin (50 to 70%), gallic acid and the like. It is used for convergence, branching, diarrhea, diarrhea, sputum, diabetes, hypothermia, anemia.

The pine tree of the present invention is an evergreen needlework tree of the gymnosperm and the pine tree, and is also called a brush, a pine tree, and a thistle tree. The stem is 35m high and 1.8m diameter, the bark is reddish brown, and the bottom is black brown. Needle leaves are 2 ~ 8cm long and 1.5mm wide. Flowers bloom in May, and male flowers hang on the bottom of new branches and are oval with a length of 1cm in yellow. The female flower runs on the tip of a new branch and is purple and egg-shaped with a length of 6mm. The fruit is ovate, 4.5cm long, 3cm in diameter, 70-100 pieces of fruit, ripen in yellowish brown color from September to October of next year. Among them, pine leaves contain glycoconjunate, which has a blood sugar lowering effect and rich in iron. It also contains beta-carotene and tetrapentin, which prevents aging and accumulation of cholesterol in the body.

Chrysanthemum indicum of the present invention is distributed in Korea, Taiwan, China, Japan and the like and mainly grows in mountains. The whole grass has short hairs, the height of the stem is 60 ~ 90cm, and it is thin and long black. Leaves are dark green, alternate phyllotaxis, egg-shaped, usually split into quadrants and pointed. The split pieces are long oval and have sawtooths with pitted edges. From September to October, the head of the stem is hung in a mountain trail. Flowers are about 2.5cm in diameter.

The gold of the present invention has a conical shape, twisted and bent with roots, and has a length of 8 to 25 cm and a diameter of 1 to 3 cm. Outer surface is yellowish-brown to dark yellow, and lump-like fine roots are sparse. On the upper side there are relatively rough, twisted, curved vertical wrinkles or irregular net patterns. The vagina is hard, fragile and easy to cut. The cut surface is yellow and the center is reddish brown. The old age is decayed or empty in the center of the roots and dark brown to reddish brown. The former is called now (枝 芩) and the latter is called 枯. (枯 芩). There is little smell and taste is a little bit.

The present invention relates to a method for producing an extract of Pseudomonas sp., A pine leaf extract, a mung bean extract and a gold extract. The extract of the present invention is obtained by leaching and transferring from a dwarf, a pine leaf, a cuttlefish and a gold or a concentrate obtained by partially or wholly concentrating the leaching solution, An extract obtained by drying water and a chemical substance itself exhibiting a main effect contained in the extract.

The present invention relates to a method for the treatment and prevention of obesity, pine needles, pine needles, and golden pine leaves, Is extracted with at least one solvent selected from the group consisting of methane, hexane, petroleum ether and diethyl ether.

The dried powder of each of the rhizome, pine leaf, truffle, and golden is dipped in the solvent at 15 to 50 ° C for 5 to 72 hours, and then extracted with an extractor equipped with a cooling condenser For 5 to 72 hours, and then concentrated under reduced pressure at 50 占 폚 or less, followed by lyophilization of the reduced-pressure concentrate. At this time, it may include a step of low-temperature aging at 4 to 15 ° C for 1 to 10 days after the extraction process.

The rhus verniciflua extract, pine needle leaf extract, guar gum extract and golden extract of the present invention may be extracted with 100 to 1500 parts by weight of a solvent for 100 parts by weight of the dried powder of each of the rhizome, pine leaf, gingko and golden.

The composition may contain 0.01 to 10% by weight of at least one member selected from the group consisting of Rhizome extract, Pine needle leaf extract, Rhizopus root extract and Golden extract, based on the freeze-dried weight of the whole composition. If the content is less than 0.01% by weight, the antibacterial activity may be insufficient. If the content is more than 10% by weight, problems such as precipitation and precipitation of the contents may occur.

Those skilled in the art will be able to control the amount of the solution containing the extract contained in the cosmetic composition of the present invention within the above range if the extract is in the form of a solution containing the respective extracts.

The cosmetic composition of the present invention may contain, in addition to the above extract, an adjuvant which can give a synergistic effect to the main effect within a range that does not impair the intended main effect of the present invention.

The adjuvant may be a conventional adjuvant known in cosmetics, such as a hydrophilic or lipophilic gelling agent, a skin softening agent, a hydrophilic or lipophilic active agent, a preservative, an antioxidant, a solvent, a perfume, a filler, .

These various adjuvants are used in amounts conventionally used in the art, and preferably 0.01% to 20% by weight based on the total weight of the composition.

Depending on their nature, adjuvants can be introduced into the lipid, aqueous, lipid vesicles and / or nanoparticles. The adjuvant and the amount thereof to be used are selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

In a preferred embodiment of the present invention, a skin-softening agent is used. Specifically, glyceryl caprylate was used, and glyceryl caprylate is extracted from natural materials and exhibits excellent antibacterial activity against bacteria while acting as a skin softening agent.

The composition of the present invention may further comprise an excipient. The excipient is typically used in cosmetics, and typical examples thereof include water, physiological saline, glycerol, and the like.

The cosmetic composition of the present invention may be in the form of an oil dispersion in an aqueous phase using an aqueous, aqueous-alcoholic or oily solution, oil or water or multiple emulsions, aqueous or oily gels, liquid, pasty or solid anhydrous products, And may be in the form of a lipid vesicle, preferably in ionic and / or non-ionic form.

The cosmetic composition of the present invention may be a fluid and may have the appearance of a white or colored cream, ointment, milk lotion, serum, essence, paste or mousse.

The cosmetic composition of the present invention may optionally be applied to the skin in the form of an aerosol, or may be in the form of a solid, for example, a stick.

The cosmetic composition of the present invention can be used as a skin care product and / or a makeup product.

Specifically, the cosmetic composition of the present invention can be used as a foundation cosmetic such as a soft lotion, a milk lotion, a nutrition cream, a massage cream, an essence, a cleansing foam, a cleansing water, a pack or a body oil; And color cosmetics such as foundation, lipstick, mascara or make-up base.

The cosmetic composition of the present invention can be used as a detergent such as a cleanser or a bath agent.

The second aspect of the present invention provides a wet tissue solution composition, which further comprises a surfactant and a moisturizing agent in the natural preservative cosmetic composition and has physicochemical stability.

The surfactant is not particularly limited as long as it can be used in a conventional cosmetic composition. For example, PEG-40 hydrogenated castor oil, lecithin, and saponin may be used. But is not limited to.

The moisturizing agent is not particularly limited as long as it can be used in a conventional cosmetic composition, and examples thereof include caprylic glycol, glyceryl caprylate, 1,3-propanediol, 1,2-hexane But are not limited to, at least one selected from the group consisting of diol, ethylhexyl glycerin, glycerin, 1,3-butylene glycol, dipropylene glycol, propylene glycol and sorbitol.

Of these, caprylyl glycols are extracted from natural substances, are hypoallergenic, exhibit high moisture-retaining ability, and are excellent in antibacterial activity. In addition, 1,2-hexanediol is preferred because it exhibits excellent antimicrobial activity against fungi and has excellent physico-chemical stability. Ethylhexyl glycerin is a vegetable glycerin derived from natural origin, and is preferable because it exhibits high moisture resistance.

In a preferred embodiment of the present invention, the wet tissue solution composition of the present invention comprises, based on the total amount of the composition, 50.0-99.05% by weight of water; 0.5-10.0% by weight of 1,3-propanediol; 0.1 to 10.0% by weight of glyceryl caprylate; 0.1-20.0% by weight of Caprylyl Glycol; 0.1 to 10.0% by weight of ethylhexylglycerin; 0.1-10.0% by weight of 1,2-hexanediol; 0.01-5.00 wt% PEG-40 Hydrogenated Castor Oil; Rhus Semialata Gall Extract 0.01-10.0 wt%; 0.01-10.0% by weight of Pinus Densiflora Leaf Extract; Chrysanthemum Indicum Extract 0.01-10.0% by weight; And 0.01-10.0% by weight of a golden extract (Scutellaria Baicalensis Root Extract).

In addition, the third aspect of the present invention provides a method for preparing a wet tissue solution composition comprising adding a surfactant and a humectant to a natural preservative cosmetic composition of the present invention, dispersing the mixture after warming, and rapidly cooling the micelles to form encapsulated micelles do.

The natural preservative cosmetic composition according to the present invention not only exhibits an excellent antibacterial activity but also has excellent antiseptic ability against cosmetics due to its broad antibacterial spectrum, It has physico-chemical stability.

The wet tissue containing the natural preservative cosmetic composition of the present invention is a natural wet tissue composed of only natural ingredients and has excellent skin stability and antibacterial power as well as excellent detergency and moisturizing power as compared with conventional chemical preservatives. In spite of containing plant extracts, it does not show specific color and specific odor inherent to plant extracts, which can enhance consumers' feeling of use.

Hereinafter, the present invention will be described in detail with reference to the following examples and experimental examples. However, the present invention is not limited thereto.

Example 1: Preparation of Natural Antibacterial Extract Composition

After shredding, the shrubs, pine needles, pine needles, and gold were refluxed with 70% (v / v) ethanol aqueous solution three times for 5 hours, cooled, and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure at 50 DEG C or lower and then dried using a freeze dryer to obtain 41.3 g of a natural antibacterial extract composition.

(Comparative Example 1), pine leaf extract (Comparative Example 2), umami extract (Comparative Example 3), and gold extract (Comparative Example 4) were prepared in the same manner as described above in order to compare the characteristics of the natural antibacterial extract composition Respectively.

Example 2: Preparation of solution of natural antibacterial extract composition

After shredding, the shaded dwarf pine needles, pine needles, tung branches and gold were extracted with 40% (V / V) 1,3-butylene glycol for 2 hours at room temperature, filtered through Whatman # 5 filter paper, g.

Example 3: Preparation of emulsion containing natural antibacterial extract composition

Emulsions were prepared with the compositions shown in Table 1 below.

Specifically, an emulsion containing the natural antimicrobial extract prepared in Example 1 was prepared. In order to evaluate the emulsion containing the natural antimicrobial extract composition of the present invention, the extracts of Opuntia ficus-indica, Pinus densiflora, Pinus densiflora extract, and gold extract were separately prepared according to the method described in Example 1, 4 was prepared.

≪ Comparative Example 5 >: An emulsion prepared in the same manner as in Example 3, except that the emulsion obtained in Comparative Example 1 was used

≪ Comparative Example 6 >: An emulsion prepared in the same manner as in Example 3, except that the emulsion of pine leaf extract of Comparative Example 2 was used

≪ Comparative Example 7 >: An emulsion prepared in the same manner as in Example 3, except that the emulsion obtained in Example 3 was used

≪ Comparative Example 8 >: An emulsion prepared in the same manner as in Example 3, except that the emulsion of the golden extract of Comparative Example 4 was used

division ingredient weight% end Purified water 70.0% Disodium iodide 0.01% Panthenol 0.2% Betaine 3.0% Butylene glycol 4.0% glycerin 3.0% I Butylated hydroxytoluene 0.02% Stearic acid 0.5% Cetearyl alcohol 0.5% Glyceryl itateate 0.3% Polysorbate 60 0.6% Methyl glucose sesquistearate 1.3% Polyethylene glycol monostearate 1.0% Butyodorpium PARKII 2.0% Tocopheryl acetate 0.1% Mineral oil 10.0% Dimethicone 0.3% Acrylate / sodium acrylamide copolymer 0.9% Cyclomethicone 1.0% All Triethanolamine 0.1% Purified water 1.0% la Natural antibacterial extract composition 0.5% Purified water Balance

Example  4: Preparation of wet tissue solution composition

0.1 to 20.0% by weight of caprylyl glycol having a solid phase wax formulation and 1.0 to 10.0% by weight of glyceryl caprylate were dissolved in a water bath at 60 ° C in a water bath, 0.01 to 5.0% by weight of PEG-40-hydrogenated caster oil, 0.5 to 10.0% by weight of 1,3-propanediol, 0.1 to 10.0% by weight of 1,2-hexanediol as a moisturizing agent, 0.1 to 10.0% by weight of ethylhexyl glycerin and 0.05 to 10.0% by weight of the solution of the natural antibacterial extract composition prepared in Example 2 were dispersed in an ice bath and rapidly dispersed through an agitator to form capsule-shaped micelles And a natural antimicrobial extract composition was collected therein to prepare a wet tissue solution composition in which the active ingredient was stably maintained.

For the evaluation of the wet tissue solution composition of the present invention, the compositions of Comparative Examples 9 to 12, in which some of the wet tissue solution compositions of Example 4 were not included, were prepared in the same manner as in Example 4.

≪ Comparative Example 9 &

A wet tissue solution composition was prepared in the same manner as in Example 4, except that caprylil glycolated was not contained.

≪ Comparative Example 10 &

A wet tissue solution composition was prepared in the same manner as in Example 4, except that glyceryl caprylate was not included.

≪ Comparative Example 11 &

A wet tissue solution composition was prepared in the same manner as in Example 4, except that 1,2-hexanediol and 1,3-propanediol were not included.

≪ Comparative Example 12 >

A wet tissue solution composition was prepared in the same manner as in Example 4, except that ethylhexyl glycerin was not included.

Experimental Example  1: Antimicrobial activity measurement

In order to measure the antibacterial activity of the antimicrobial extract of the present invention, the minimum inhibitory concentration was measured using a solid culture dilution method (Agar Serial Dilution Method). Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027), Candida albicans ATCC 10231 (yeast), Candida albicans ATCC 10231, and Fusarium spp. As a fungus were used as Gram-positive bacteria, Staphylococcus aureus ATCC 6538P, (Aspergillus niger ATCC 22343) was used.

In the case of bacteria, the bacteria are inoculated on a tryptic soy broth and cultured at 37 ° C for 24 hours. In the case of yeast, the bacteria are inoculated into a potato dextrose broth and cultured at 25 ° C The fungi were inoculated on a Potato dextrose agar and incubated for 7 days in a 25 ° C. incubator. Spores of filamentous fungi formed on the surface of the medium were recovered using a sterilized cotton swab And diluted in sterilized saline.

2 ml of each of the extracts of Example 1 and Comparative Examples 1 to 4 diluted to a proper concentration in 5% Dimethylsulfoxide (DMSO) physiological saline solution was added to the sterilized Petri dish and the control group was diluted with 5% Dimethylsulfoxide physiological saline solution And then sterilized in each Petri dish, and 18 ml of a tryptic soy agar medium and a potato dextrose agar medium cooled to 48 ° C were added to each Petri dish, and the mixture was mixed well and allowed to stand and solidify.

Then, each of the pre-cultured bacteria was applied to each petri dish at a final concentration of about 1 × 10 ⁶ CFU, and in the case of yeast, each petri dish at a concentration of about 1 × 10 ⁵ CFU , And in the case of filamentous fungi, the flora was applied to each petri dish at a bacterial concentration of about 1 × 10 ⁴ CFU. Each petri dish was incubated at 37 ° C for 24 hours in the case of bacteria, for 3 days in a 25 ° C incubator for yeast, and for 7 days in a 25 ° C incubator for filamentous fungi. The minimum inhibitory concentration (MIC) was defined as the minimum antimicrobial concentration of the plate which was not allowed to grow.

division Minimum inhibitory concentration (% by weight) S. aureus E. coli P. aeruginosa C. albicans A. niger Example 1 (Natural antibacterial extract composition) 0.1 0.025 0.04 0.4 0.2 Comparative Example 1 (Rhizoma extract) 0.1 0.2 0.5 or more 1.0 or higher 1.0 or higher Comparative Example 2 (pine leaf extract) 0.15 0.2 0.4 0.5 or more 0.5 Comparative Example 3 (hamcho extract) 1.0 or higher 2.0 or higher 1.0 or higher 0.025 0.4 Comparative Example 4 (Golden extract) 2.0 or higher 2.0 or higher 2.0 or higher 0.4 0.1

As shown in Table 2, in the case of Example 1 (a natural antibacterial extract composition), 0.025% by weight or less with respect to E. coli and 0.04% by weight or less with respect to P. aeruginosa as compared with Comparative Examples 1 to 4 %, Respectively. In addition, C. albicans or A. niger showed similar or good antimicrobial activity to the comparative example.

Experimental Example 2: Evaluation of emulsion stability

The emulsion stability of the emulsion of Example 3 and Comparative Examples 5 to 8 was stored at high temperature (45 ° C) for 6 months and observed And the results are shown in Table 3. < tb > < TABLE >

division Example 3 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Viscosity 2100 cps 1800 cps 2400 cps 2250 cps 1900 cps Emulsion stability No phase separation Weak phase separation No phase separation No phase separation No phase separation

As shown in Table 3, phase separation did not occur in the milky lotions of Comparative Examples 5 to 8 using the individual extracts as well as the emulsion of Example 3, but slight phase separation occurred only in Comparative Example 5 (the extract of the rhizome). Therefore, the emulsion stability of the emulsion containing the natural antibacterial composition of the present invention is good.

Experimental Example  3: Evaluation of the antimicrobial activity of the wet tissue solution composition

For each of the compositions of Example 4 and Comparative Examples 9 to 12, 50 ml of water was added to the vial and the test solution was prepared by adding 1.0% by weight of the composition.

9 ml of saline solution was added to the test tube, and Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and fungus Aspergillus niger were added and cultured for testing Was prepared.

Immediately after preparing a sample, 0.1 ml of test mixture was added to 50 ml of each test solution. The sample was inoculated into a TSA (Tryptic soy agar) medium and a PDA (Potato dextrose agar) medium and incubated in an incubator at 30 ° C for 24 hours And the initial number of bacteria was measured.

The colony was counted by inoculating the TSA medium and the PDA medium in an incubator at 30 ° C for 24 hours in the same manner as described above after the sample was set for 24 hours. Table 4 shows the results.

The result of measuring the sterilization power of the wet tissue solution composition Example 4 Comparative Example 9 Comparative Example 10 Comparative Example 11 Comparative Example 12 Initial number of bacteria TNTC (1x10 or more) One-day politics
Samples (TSA medium) cfu <10 > 1000 > 1000 <1000 > 1000 Sterility (%) > 99.99 Almost none Almost none 90 Almost none One-day politics
Sample (PDA medium) cfu <10 > 1000 > 1000 <1000 > 1000 Sterility (%) > 99.99 Almost none Almost none 90 Almost none TNTC: Too Numerous To Count
cfu: colony forming unit

Comparative Example 9: Not containing caprylyl glycol

Comparative Example 10: Not containing glyceryl caprylate

Comparative Example 11: No 1,2-hexanediol and 1,3-propanediol

Comparative Example 12: Not containing ethylhexyl glycerin

As shown in Table 4, the initial number of bacteria was observed as TNTC (about 10 to 10 cfu) in each group, but when the sample was incubated for one day in a medium after standing for one day, the number of microorganisms was 10 And in Comparative Examples 9 to 12 in which some components of the wet tissue solution composition were not included, a large amount of microorganism population was observed, and even when the natural antibacterial extract composition of Example 1 was included in the wet tissue solution composition, It has been confirmed that it still has an effective antibacterial activity if it is contained in an appropriate ratio with Cole, glyceryl caprylate, 1,2-hexanediol and 1,3-propanediol, and ethylhexyl glycerin.

Experimental Example 4: Evaluation of physicochemical stability according to the composition preparing method

In order to evaluate the physico-chemical stability of the wet tissue solution composition of the present invention, 1,3-propanediol and 1,2-hexanediol, ethylhexyl glycerin, caprylyl glycols, glyceryl caprylate, -Hydrogenated castor oil was first mixed and then the antibacterial extract composition of Example 1 was simply added to prepare a composition (Comparative Example 13).

To the vial, 1% (v / v) solution was prepared by mixing 99 ml of purified water and 1 ml of the composition of Example 4. To the vial, 99 ml of purified water and 1 ml of the composition of Comparative Example 13 were mixed to prepare a 1% Respectively. 8 pieces were prepared. Next, the stability of each sample was visually observed at 0 ° C, 25 ° C, 40 ° C and 60 ° C.

0 ℃ 25 ℃ 40 ℃ 60 ° C Example 4 Transparency Transparency Transparency opacity Comparative Example 13 Transparency Transparency Slightly opaque opacity

As shown in Table 5, it can be seen that the composition of the wet tissue solution (Example 4) prepared according to the present invention is more stable at 40 ° C than the composition of Comparative Example 13. This result is meaningful because it can affect the quality of the product due to the seasonal change of temperature due to the increase of temperature around 40 ℃.

Experimental Example 5: Evaluation of bubble generation

The solution to which the wet tissue solution composition of the present invention (Example 4) and the composition of Comparative Example 13 were respectively applied was shaken at 180 rpm under the condition of a vertical shaking type extraction stirrer for about 1 minute, and then allowed to stand for about 15 seconds to evaluate the height of foam generation . As a result, the results shown in Table 6 were obtained.

The generation of bubbles is caused by the surfactant. When the bubbles are large, bubbles are generated in the process, and it is difficult to produce a normal product. Especially, in a pop-up form, a sticking effect occurs between the nonwoven fabric and the nonwoven fabric, , Which is a serious consumer complaints issue and is one of the important items in the review of preservatives.

Height of foam after 1 minute after stopping Height of foam after 5 minutes after stopping Height of bubble after 10 minutes after stop Example 4 5cm 3cm 2cm Comparative Example 13 10cm 6cm 5cm

As shown in Table 6, the wet tissue using the wet tissue solution composition of the present invention (Example 4) retained little foaming even after a relatively long time.

Experimental Example 6: Evaluation of moisture

The moisture retention of the wet tissue using the composition of Example 4 and the composition of Comparative Example 13 prepared in Experimental Example 4 was evaluated. The results are shown in Table 7 below. For this purpose, a wet tissue was applied to the left and right arm parts of the human body over time to perform a corneometer analysis.

time Comparative Example 13 Example 4 0 minutes 58.1 57.8 1 minute 66.1 66.7 10 minutes 55.7 56.8 30 minutes 48.6 51.1 1 hours 42.5 50.7

* Unit (A.U.: Arbitray unit, unit constant, skin moisture content)

As shown in Table 7, the wet tissue using the wet tissue solution composition of the present invention (Example 4) remained moisturized even after a relatively long time.

In addition, the relative wetness of the wet tissue using the wet tissue solution composition of the present invention remained high over time.

Generally, the preservative ingredient of the wet tissue is mainly composed of purified water, causing skin dryness after use, generally causing fine keratinization or rough skin surface to cause slight red spot or itching symptoms, When the wet tissue solution composition is used, it is possible to maintain a moisturizing moisture and a skin barrier to prevent it.

Experimental Example  7: With special colors Peculiarity  Change result

To evaluate changes in specific color and specific odor with time of the wet tissue solution composition of the present invention, a 1% (v / v) solution was prepared by mixing 99 ml of purified water and 1 ml of the composition of Example 4 in a vial , A 1% (v / v) solution was prepared by mixing 99 ml purified water with 1 ml of the composition of Comparative Example 13 in a vial.

Next, for each sample, whether or not a color change and a specific change occurred about 1 month under the conditions of 0 ° C, 25 ° C, 40 ° C and 60 ° C and under irradiation conditions such as sunlight (sunlight) And it was confirmed by measuring with the smell.

0 ℃ 25 ℃ 40 ℃ 60 ° C daylight Ultraviolet light Infrared light Example 4 No specific color change
No specific change No specific color change
No specific change No specific color change
No specific change No specific color change
No specific change No specific color change
No specific change No specific color change
No specific change No specific color change
No specific change Comparative Example 13 No specific color change
No specific change Occurrence of specific color change
Occurrence of specific odor change Occurrence of specific color change
Occurrence of specific odor change Occurrence of specific color change
Occurrence of specific odor change Occurrence of specific color change
Occurrence of specific odor change Occurrence of specific color change
Occurrence of specific odor change Occurrence of specific color change
Occurrence of specific odor change

As shown in Table 8, the wipes solution composition (Example 4) prepared according to the present invention has no specific color change and specific odor change as compared with the composition of Comparative Example 13, so that a more stable quality can be maintained.

Claims (12)

A natural preservative cosmetic composition comprising a rhizome extract, a pine leaf extract, a rhizome extract and a golden extract. The cosmetic composition according to claim 1, wherein at least one member selected from the group consisting of Rhizoma extract, Pine leaf extract, Rhizopus root extract and Gold extract is included in the composition in an amount of 0.01 to 10% by weight based on freeze- Composition. The method according to claim 1, wherein the rhizome extract, pine needle leaf extract, rhizome extract, and gold extract are prepared by dipping 5 to 72 hours of the dried powder of each of the rhizome, pine leaf, 72 hours, and then concentrated under reduced pressure at 50 占 폚 or lower, and lyophilized the reduced-pressure concentrate. The method according to claim 1, wherein the rhus verniciflua extract, pine needles leaf extract, rhizome extract and gold extract are each extracted with 100 to 1500 parts by weight of a solvent relative to 100 parts by weight of dried powder of each of rhizomes, pine leaves, Cosmetic composition. 4. The method of claim 3, wherein the solvent is selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, propylene glycol, chloroform, ethyl acetate, dichloromethane, hexane, petroleum ether, By weight or more, of a natural preservative cosmetic composition. The cosmetic composition of claim 1, wherein the cosmetic composition is an aqueous, aqueous-alcoholic or oily solution, an oil-in-water or water-in-oil or multiple emulsion, an aqueous or oily gel, a liquid, a pasty or solid anhydrous product, Oil dispersions, lipid vesicles in ionic or non-ionic form. The cosmetic composition according to claim 1, wherein the cosmetic composition is used as a flexible lotion, a milk lotion, a nutritional cream, a massage cream, an essence, a cleansing foam, a cleansing water, a pack, a body oil, a foundation, a lipstick, a mascara, Preservative cosmetic composition. The composition according to claim 1, wherein the composition comprises,
50.0-99.05 wt% water;
0.5-10.0% by weight of 1,3-propanediol;
0.1 to 10.0% by weight of glyceryl caprylate;
0.1-20.0% by weight of Caprylyl Glycol;
0.1 to 10.0% by weight of ethylhexylglycerin;
0.1-10.0% by weight of 1,2-hexanediol;
0.01-5.00 wt% PEG-40 Hydrogenated Castor Oil;
0.01 to 10.0% by weight of Rhus verniciflua extract;
Pine leaf extract 0.01-10.0 wt%;
0.01-10.0% by weight of hamsters extract; And
0.01-10.0% by weight of the golden extract
&Lt; / RTI &gt; The wet tissue solution composition according to any one of claims 1 to 8, further comprising a surfactant and a moisturizing agent in the natural preservative cosmetic composition and having physicochemical stability. 10. The wet tissue solution composition according to claim 9, wherein the surfactant is at least one selected from the group consisting of PEG-40 hydrogenated castor oil, lecithin and saponin. 10. The composition of claim 9, wherein the humectant is selected from the group consisting of caprylyl glycol, glyceryl caprylate, 1,3-propanediol, 1,2-hexanediol, ethylhexyl glycerin, glycerin, Butylene glycol, dipropylene glycol, propylene glycol, and sorbitol.  8. A method for preparing a wet tissue solution composition, comprising adding a surfactant and a humectant to the natural preservative cosmetic composition of any one of claims 1 to 8, and dispersing the mixture after warming, followed by rapid cooling to form encapsulated micelles.