KR101650565B1 - Water tissue having the freshness - Google Patents

Abstract

The present invention relates to a wet tissue having a refreshing feel, and it includes a liquid composition including purified water, ethanol, menthol, and trehalose, and a wet tissue fabric so that an explosive refreshing feeling and a refreshing feeling can be continuously felt The present invention also relates to a wet tissue having a refreshing sensation capable of moisturizing skin, calming skin, decomposing waste matter and cleansing effect through various kinds of skin conditioning compositions.

Description

Water tissue having the freshness "

The present invention relates to a wet tissue having a refreshing sensation, and more particularly to a liquid composition capable of providing a refreshing refreshing feeling without skin irritation, in particular menthol, ethanol and trehalose, and optionally a white cotton flour containing phytoncide components Is wetted with a wet tissue so that when a user who sweats a lot of time, such as outdoor activities or athletic activities, wipes the skin using the wet tissue, the wet tissue has a refreshing feeling that can continuously feel an explosive freshness, a cooling sensation and a cooling sensation for a long time.

In addition, the present invention can expect skin moisturizing, skin soothing, decomposition and cleansing effects through various kinds of skin conditioning compositions contained in the liquid composition, and it is possible to produce halozone, To a wet tissue having a cooling sensation with a strong buoyant force to be killed.

Generally, a wet tissue is a disposable towel which is formed by absorbing various types of liquid compositions on a wet tissue such as a pulp or nonwoven fabric. In the case of an adult, it is used for keeping hands or skin clean, The sweat is wiped off when sweating through the back of the sweater to induce a decrease in body temperature due to the vaporization of water, thereby being used for the purpose of feeling freshness and refreshing feeling.

Since such a wet tissue is rubbed directly on the skin, it is excellent in safety against skin, and it is easy to remove foreign matter or pathogenic microorganisms remaining on the skin, and should be excellent in antibacterial property.

In addition, in order for the user to feel freshness and refreshing feeling, the components constituting the liquid composition should be composed of ingredients having a refreshing and refreshing feeling.

However, in the conventional wet tissue, menthyl lactate and ethanol are used to give refreshing sensation, but menthyl lactate and ethanol alone can not give explosive refreshing feeling and refreshing feeling, and because of the nature of ethanol, Can not be sustained for a long time.

In order to solve the problems of the conventional wet tissue, the present inventors have found that a liquid composition capable of providing a refreshing refreshing feeling without skin irritation, in particular menthol, ethanol and trehalose, and optionally a white cotton flour containing phytoncide components Is impregnated with a wet tissue, it is possible to continuously feel explosive refreshing feeling and refreshing feeling for a long time when a user who sweats a lot, such as outdoor activity or athletic activity, wipes the skin using a wet tissue, Inventing a wet tissue having a refreshing sensation with a strong repulsion force that can expect skin moisturizing, skin soothing, cleansing and cleansing effects through a conditioning composition, and halosone generation to prevent harmful bacteria from being killed and cultured .

Korean Patent Publication No. 1998-0008247

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and it is an object of the present invention to provide a liquid composition capable of giving a refreshing refreshing feeling without skin irritation, particularly cottonwood flour containing menthol, ethanol and trehalose, and optionally phytoncide ingredient, The present invention is to provide a wet tissue having a refreshing feeling that can continuously feel an explosive refreshing feeling and a cooling sensation for a long time when a user who sweats a lot, such as outdoor activities or athletic activities, wipes the skin using the wet tissue.

In addition, the present invention can expect skin moisturizing, skin soothing, decomposition and cleansing effects through various kinds of skin conditioning compositions contained in the liquid composition, and it is possible to produce halozone, And to provide a wet tissue having a cooling sensation with a strong buoyant force to be killed.

A wet tissue having a refreshing sensation according to an embodiment of the present invention includes a liquid composition including purified water, ethanol, menthol, and trehalose; And a wet tissue fabric.

Here, since the liquid composition can be impregnated with the wet tissue, the wet tissue can be fabricated into a fabric having a high moisture absorptivity to easily absorb the liquid composition.

The moisture absorptivity of the liquid composition to the wet tissue fabric may be 70 to 80 percent, wherein the moisture absorption rate of the liquid composition to the wet tissue fabric is 70 to 80 percent, It is meant that an area of 70 to 80 percent (%) of the total area of the wet tissue fabric in the dry state (e.g., 19 cm x 20 cm) can be impregnated through the liquid composition.

At this time, when the moisture absorption rate of the liquid composition is 70% (%) or less, it is a soft type, and when the skin is wiped, it may be a hypo-hygroscopic wet tissue having a somewhat lower refreshing, cooling and refreshing feeling.

When the moisture absorption rate of the liquid composition is 80% or more, it is a hard type. When the skin is wiped, the refreshing feeling, the cooling feeling and the refreshing feeling explosively increase, .

At this time, the user may feel a slight feeling of being made up.

On the other hand, a detailed description of the liquid composition described above is as follows.

Purified water refers to water purified by distillation of a constant or through an ion exchange resin, and can mainly serve as a solvent or a skin conditioner.

At this time, the weight percentage (%) of the purified water with respect to the total weight percentage (%) of the liquid composition may correspond to 72.644 to 88.787 weight percent, and preferably 80.715 weight percent.

Next, ethanol is a colorless liquid with volatility and flammability, which is an alcohol in which two hydrocarbons in the form of hydrocarbons are substituted by one hydrogen atom in the ethane with two hydrogens. It is also called ethyl alcohol and is also called alcohol because it is the main component of alcohol. The molecular formula is C2H5OH, and chain hydrocarbons are also called aliphatic hydrocarbons and have different names depending on the number of carbons.

This ethanol is an aliphatic hydrocarbon derivative in which a hydrogen atom is replaced with a hydroxyl group (-OH) in ethane (C2H6) having two carbon atoms in an aliphatic hydrocarbon compound. This ethanol is colorless and has a unique flavor. It has a melting point of -114 degrees, a boiling point of 78 degrees, and a specific gravity of 0.7893.

The alcohol has a characteristic of forming a hydrogen bond between molecules, and the boiling point of ethanol is much higher than the boiling point of ethane of the same carbon number of -89 degrees, and the alcohol having a hydroxyl group as a functional group is a very stable compound . Also, since the hydroxy group, which is the functional group of the alcohol, does not ionize, the alcohol is a neutral substance.

This ethanol is mixed with organic solvent or water such as other alcohol, ether, or chloroform at an arbitrary ratio. Distillation of the mixture with water does not produce pure ethanol, and 96.0% (%) of ethanol and 4.0% (%) of water in the azeotropic mixture are distilled.

Moreover, such ethanol is easy to burn, and when ignited, it burns with a flame without color. It can explode if it burns in steam. If it oxidizes, it becomes acetic acid through acetaldehyde. It has a sterilizing effect because it has a property of solidifying proteins. At this time, the sterilizing power is equal to 70% (%) of the maximum amount of aqueous solution, and 60% (%) or less and 80% (%) or more have almost no disinfection and sterilizing power. It is naturally present in the form of an ester of a carbonylic acid and is not present in the free state.

On the other hand, such ethanol was prepared by a method in which sugar was fermented by a yeast. However, the price of molasses, which is a raw material, is high, and half of the raw materials are converted to carbon dioxide, which is a waste. However, alcoholic beverages are still produced almost by fermentation.

As a synthesis method of ethanol, a sulfuric acid addition method in which sulfuric acid ester obtained by absorbing ethylene into sulfuric acid is hydrolyzed and obtained together with ether is carried out. In this step, 90% (%) of ethylene as a raw material can be obtained together with ether . However, since a large amount of sulfuric acid is concentrated and circulated, a large-scale facility is required. It is also possible to synthesize by iodine synthesis method.

Such ethanol is mainly provided as a mainstream alcoholic beverage, and contains 15 to 16 percent (%) of sake, 3 to 4 percent (%) of beer, and 35 to 55 percent (%) of distilled liquor such as whiskey or brandy .

In addition, ethanol plays an important role as a solvent and a starting material for synthesis, and can be used for the production of ether, chloroform, iodine foam, acetic acid, acetaldehyde, ethyl chloride and the like. It is also used as an analytical reagent in addition to being used as a tincture and a drug for medicine. At this time, industrial alcohols use denatured alcohol with a small amount of toxic methanol, and are prohibited as beverages.

On the other hand, the weight percentage (%) of ethanol with respect to the total weight percentage (%) of the liquid composition described above may correspond to 8 to 12 weight percent (%), and preferably corresponds to 10 weight percent (%).

In this case, when the weight percentage (%) of ethanol is equal to or less than 8, the vaporization function of ethanol may be deteriorated. If the weight percentage is equal to or more than 12, users may feel discomfort due to a strong incense unique to ethanol. To 12% by weight (%) of ethanol.

Next, menthol is also referred to as the peppermint brain. The formula is C10H20, the molecular weight is 156.27, the melting point is 41.6 degrees, and the specific gravity is 0.890 (15 degrees). Such menthol is naturally spontaneously rotated L-menthol as a major component of the peppermint oil. Menthol is a colorless, needle-like crystal with a unique sense of exhilaration. It is almost insoluble in water but dissolves well in ethanol, ether and chloroform.

Such menthol can be obtained by steam distillation of leaves or stalks of peppermint, and is easily synthesized by adding water to ketone groups or double bonds of menthone, pregon, piperitone, thymol and isoprene. Conventional synthetic menthol is often a mixture of isomers, and is less than the natural mint in the melting point and aroma. In recent years, however, the refined method has been developed to obtain synthetic L-menthol that is as good as natural products.

These menthol can feel the tongue sticking feeling if you put a lot of it into your mouth, but you can feel a refreshing feeling when it is small. It is also added to medicines, confectionary and cosmetics, and is also used to stop painkillers and itching. In addition to L-menthol, D-menthol and DL-menthol are also known, but not naturally. The DL body has a melting point of 35 to 36 degrees, which is generated when thymol is reduced, and the D body can be separated from the DL body.

On the other hand, the weight percentage (%) of menthol to the total weight percentage (%) of the liquid composition described above may correspond to 1.316 to 1.974 weight percent (%), and preferably 1.645 weight percent (%).

When the weight percentage (%) of menthol is less than 1.316, the refreshing aroma and refreshing feeling unique to menthol may be deteriorated. When the weight percentage is 1.974 or more, the refreshing aroma and refreshing feeling unique to menthol are strong, It is preferable to apply 1.316 to 1.974 weight percent (%) menthol.

On the other hand, menthol can maintain a refreshing sensation felt by the user's skin. The sensation of cooling can last for about 20 to 40 minutes. When the user applies wind, the sensation of refreshing, cooling, I can feel it.

Next, trehalos protects the cell membrane and can prevent moisture evaporation. It is a type of saccharide that exists in nature in a wide range from microorganisms to plants and insects. Non-reducing saccharides having this structure to be.

It has been found that trehalose is a saccharide that exhibits the function of inhibiting the degeneration of the three major nutrients in the body such as suppression of starch aging, inhibition of protein denaturation, and inhibition of lipid degradation. The hardness of Baeksulgi decreased as the amount of trehalose increased, and the hardness increased rapidly until 3 days of storage.

In addition, the trehalose and ceramide ingredients calms the skin and provides a smooth, translucent skin. In the cactus, there is a lot of trehalose, which is a kind of trehalose.

On the other hand, the weight percent (%) of trehalose with respect to the total weight percentage of the liquid composition described above may correspond to 0.956 to 1.434 weight percent (%), preferably 1.195 weight percent (%).

Next, the cupressus funebris powder is an evergreen coniferous tree of the yucca tree, which is a gymnosperm plant, and the scientific name is a powder made by grinding the cotton tree which is Chamaecyparis obtuse.

The Japanese cypress tree is called hinoki in Japan and the cypress tree in China. It is also known as cypress, and it is well known as the species that most exudes and secretes phytoncide.

It is 40m in height and 2m in diameter. The branch is spread horizontally to form a conical water pipe. The bark is reddish brown, fibrous and thin stripped. The leaves are opposite to each other, thick, 1 to 1.5 mm in length, small like scales, and the pore-forming line on the back is Y-shaped. There is also one line on the leaf surface and a white dot on the back. The fruit is round as a cone and has a diameter of 10-12 mm. It is reddish brown and consists of 8 pieces of seeds. Each seed contains 2 seeds. The length of the seed is about 3 mm, and there are two flysches and narrow wings.

This is a Japanese specialty species, and because of its good quality, it can be cultivated as a planting species in the southern part of Korea.

On the other hand, the phytoncide ingredient contained in the unicorn tree has psychological stabilization, peripheral blood vessel training, strengthening cardiopulmonary function, bronchial asthma, treatment of pulmonary tuberculosis, and strengthening the heart. It can also be used to disinfect the skin.

In addition, phytoncide has a strong sterilization effect, which cleanses the air in the forest, and when the air is inhaled, the phytoncide inhaled through the air selectively sterilizes the bad bacteria in the human body so that the blood of the human body can be purified have. This means that the metabolism of the human body is smooth, and the head can be refreshing as well as the appetite.

The powder of the flour wood containing a large amount of such phytoncide can have a harmful substance removing effect, a strong sterilizing effect, a preservative function and a deodorizing effect.

On the other hand, there are bathtubs, foot baths, etc. for the hinoki products using the unbleached wood. It is especially effective for people with atopy due to the unique flavor and effect of hinoki. The hinoki rice can be stored for a long time without damaging the rice with moisture coming from hinoki.

On the other hand, the weight percentage (%) of the flocked powder with respect to the total weight percent (%) of the liquid composition described above may correspond to 0.624 to 0.936 weight percent (%), preferably 0.780 weight percent .

Next, the Bacillus extract promotes exfoliation along the natural skin cycle, forms a water film on the dermis layer, and can supply water.

In addition, Bacillus extract has high skin healing power and cell regeneration promoting ability, it can relieve spots and freckles and supply nourishment to skin. In addition, the long-lasting effect can be sustained. When fermenting Bacillus extract, it is possible to make an amino acid which is absorbed well on the skin and can regenerate the skin by using microorganisms. In this case, the enzyme can effectively decompose the dead skin of the skin have.

On the other hand, the weight percentage (%) of the Bacillus extract to the total weight percentage (%) of the liquid composition described above may correspond to 0.624 to 0.936 weight percent (%), preferably 0.780 weight percent .

Next, the rice bran extract may mean a crumbling mixture such as perilla, seed coat, and colony formed during the process of turning from brown rice to pure white rice.

These rice bran extracts contain various nutrients such as vitamin A, vitamins B1, B6, iron, phosphorus and minerals. In particular, vitamin B1, B6, and E are contained, which can be effective in improving skin diseases and can also help alleviate itching.

In addition, the rice bran extract has a skin cleansing action, and because it contains fat, it can be gelled and smoothed with the use of it, and the rice bran extract can be used for atopic skin with severe itching because of less skin irritation. Can also work effectively.

On the other hand, the weight percentage (%) of the rice bran extract relative to the total weight percentage (%) of the liquid composition described above may correspond to 0.624 to 0.936 weight percent (%), preferably 0.780 weight percent .

Next, soybean extract contains a large amount of natural polysaccharides and has a molecular mechanism that promotes the ability of the damaged skin to regenerate the skin.

Especially, soybean extract can effectively work on skin that is severely damaged, such as reddened skin in strong summer sunshine. Accordingly, the soybean extract inhibits moisture evaporation of the skin, plays a role as a natural moisturizing agent harmless to the skin, and protects the skin from ultraviolet rays.

On the other hand, since the natural polysaccharide is larger than the molecular size that can be absorbed by the human skin, the polysaccharide must be finely cut when it is used as a cosmetic raw material.

When the soybean extract is fermented using microorganisms, the soybean extract is absorbed into the skin without losing its efficacy and effect, and synergy effects such as a sulfation effect, an antibacterial effect and an anti-inflammatory effect can be expected.

On the other hand, the mucopoly polymer material produced from the fermented soybeans can have excellent moisture retention, high skin permeability, spreadability, spreadability, adhesion and absorbency.

On the other hand, the weight percentage (%) of the soybean extract to the total weight percentage (%) of the liquid composition described above may correspond to 0.624 to 0.936 weight percent (%), preferably 0.780 weight percent .

C. atlantica bark extract refers to an extract of four evergreen coniferous trees belonging to the pine and lobsters. Atlas cider can be used for tubular baths and woods, In the mountainous region, one species grows on the western shores of the Himalayas.

At this time, only C. deocara, C. atlantica, C. libani, and C. brevifolia, which are called cedar in English, to be. They are all arboreous, with thick tree trunks, branches spreading horizontally from the top of the tree, and small branches falling down to form irregular large lumps. Young trees are covered with dark gray, soft water, which grows brown and splits like scales. Needle-shaped hard leaves are triangular, long branches are sparse, short branches are gathered tightly at the ends and run with alcohol. Each leaf has two syringes, hanging on the tree for three to six years and falling off. The green or purple cone, which looks like a large barrel, has a lot of rosin and runs on the end of a short branch, which is tightly packed with thin and solid sculptures.

These Atlas cydas have a lot of rosaceae and are long lasting in the ground or under water. Distillation of atlas cedar also results in the production of directional oil, in which the oil is volatile and can be used as a flavoring and skin conditioner.

Many of these varieties of Atlas cedar and dogwood are planted ornamental in North America, particularly on the coast of the Pacific Ocean and the Gulf of Mexico.

On the other hand, the weight percentage (%) of the Atlas cider peel extract to the total weight percentage (%) of the liquid composition described above may correspond to 0.624 to 0.936 weight percent (%), preferably 0.780 weight percent .

Next, Asian plantain extract may be a perennial herb that belongs to Plantaginaceae.

The tea leaves have no stem, and the elliptical leaves come out from the roots and form rosettes and have long petiole. The edges of the leaves are slightly wrinkled and have indistinct sawtooths. The flower of Chaechoncho is a flower of inflorescence at the end of a peduncle which straightens between the leaves from June to August. At this time, calyx and corolla are divided into four quadrilaterals, and the surgery is long out of the flower. In addition, the seeds of the beetles are formed by capsules. When ripe, the middle part is split and the black seeds bounce out. It is also a weed growing on the sunny roads of Ghana, but in spring and summer, young seeds can be eaten as a casserole, and in the fall, seeds can be dried in the sun to create tea dielectrics and dentifrices. In addition, chewing the roots in the soil, or eating the juice of the leaves can be eaten.

These extracts are effective for mucus relaxation, antipyretic action, hemolytic action, pediatric cough and chronic bronchitis, promoting excretion of water, excretion of urea, sodium chloride and uric acid, and at the same time helping to dissolve urinary stones. It also has an anti-inflammatory action and an effect of inhibiting gastric ulcer formation.

On the other hand, the weight percentage (%) of the herbal extract based on the total weight percentage (%) of the liquid composition described above may correspond to 0.624 to 0.936 weight percent (%), preferably 0.780 weight percent .

Next, the fragrance ingredient may include sweet apple, oriental, musk fragrance and the like, and may be used to make an aquatic refreshing through appropriate harmony. The weight percentage (%) of the perfume to the total weight percentage (% ) Can be 0.44 to 0.66 weight percent (%), preferably 0.550 weight percent (%).

Next, Cetylpyridinium Chloride is an antistatic agent corresponding to a quaternary ammonium salt, and can act as an antistatic agent and a deodorant.

On the other hand, the weight percentage (%) of cetylpyridinium chloride to the total weight percentage (%) of the liquid composition described above may correspond to 0.536 to 0.804 weight percent (%), preferably 0.670 weight percent .

Next, PEG-40 is a term for polyethylene glycol, which can help various PEG species to be mixed with fatty acids and fatty alcohols.

In addition, PIGGY-40 produces substances that give various functions to cosmetics such as surfactants and binders (stabilizing agents and emollients), and they are also used for the color of pearl light. Use toxic gas to produce.

On the other hand, the weight percentage (%) of the pigment-40 to the total weight percentage (%) of the liquid composition described above may correspond to 0.4 to 0.6 weight percent (%), preferably 0.500 weight percent .

Finally, sodium dehydroacetate is a kind of antiseptic and can mainly mean a heterocyclic compound, and can act as a sterilizing preservative.

On the other hand, the weight percentage (%) of sodium dehydroacetate relative to the total weight percentage (%) of the liquid composition described above may correspond to 0.66 to 0.99 weight percent (%), preferably 0.825 weight percent .

The present invention is to provide a liquid composition capable of giving a refreshing refreshing feeling without skin irritation, in particular a cottonwood powder containing menthol, ethanol and phytoncide components, When the user who wipes wipes the skin using the wet tissue, it has an effect that the user can continuously give explosive refreshing feeling and refreshing feeling for a long time.

In addition, the present invention can provide skin moisturizing, skin soothing, decomposition and cleansing effects through various kinds of skin conditioning compositions contained in the liquid composition, and can produce halozone through strong anti-buoyancy, Since the bacteria do not grow and die, they are always kept sterile.

In addition, the present invention has an advantage that it can be safely used without worrying about skin irritation and skin harm by eliminating harmful components such as formaldehyde, lead and cadmium.

In addition, the present invention can have a skin disinfecting effect and an effect of smoothly metabolizing the human body through the phytoncide component of the flour powder.

In addition, since the present invention refreshes the skin through a unique and refreshing sensation of cooling sensation of menthol, people living in a country where high temperature weather is always maintained, such as the equatorial region, soldiers, sports players, Thereby making it possible to reduce the discomfort index by allowing people who sweat a lot to maintain a refreshing mood at all times.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

<Examples>

Throughout this specification, the percentages used to denote the concentration of a particular substance are percent by weight unless otherwise specified.

&Lt; Preparation Example 1: Preparation of liquid composition &

A liquid composition comprising purified water, ethanol, menthol and trehalose was prepared and analyzed for ingredients. According to these tests, the component analysis table of the liquid composition is shown in Table 1 below.

NO NAME Contents One Purified water 80.715 (%) 2 ethanol 10.000 (%) 3 menthol 1.645 (%) 4 Trehalose 1.195 (%) 5 PIGGY-40 Hydrogenated Castor Oil 0.500 (%) 6 Bacillus, rice bran extract, soybean extract fermentation filtrate 0.780 (%) 7 Atlas cedar bark extract 0.780 (%) 8 Flour powder 0.780 (%) 9 Pine tree pine cone extract 0.780 (%) 10 Herbal extract 0.780 (%) 11 Spices 0.550 (%) 12 Cetylpyridinium chloride 0.670 (%) 13 Sodium dehydroacetate 0.825 (%) Total 100 (%)

&Lt; Preparation Example 2: Preparation of a wet tissue containing a wet tissue impregnated in a liquid composition >

The liquid composition of Table 1 is impregnated into a wet tissue fabric (e.g., a nonwoven fabric) according to the wet tissue manufacturing process, wherein the moisture absorptivity of the liquid composition to the wet tissue fabric is 70 to 80 percent (%).

&Lt; Experimental Example 1: Measurement of repellency of liquid composition >

A test for measuring the flotation force of a wet tissue having a refreshing sensation manufactured through Production Example 2 was conducted.

Here, the buoyant force measurement experiment means a method of directly determining the repelling force of the microorganism through the reduction of the microorganism and the mortality rate by directly inoculating the microorganism into the sample. At this time, the inoculum strain is Aspergillus niger which is a mixture of three bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa).

The procedure of buoyancy measurement experiment is as follows.

First, one sheet of each wet tissue was put into 100 ml of sterilized saline to sufficiently dissolve the liquid of the tissue.

Then, Aspergillus niger, a mixture of three bacteria, was inoculated into the product at 10 ⁷ cfu / g (ml) and 10 ⁵ cfu / g (ml), respectively.

Then, the specimen was checked whether the bacteria were killed after a certain period of time.

In this case, 1 ml of the specimen is diluted in 9 ml of 0.9% (saline) solution, stained with duplicate in each step, counted in a constant-temperature incubator, counted in the number of clusters, And the number of bacteria was calculated.

Also, at this time, the antifungal ability against the mold of the wet tissue was determined by the following method.

First, Aspergillus niger (concentration of 10 ⁴ cfu / g (ml)), a mixture of three bacteria, was streaked into the PDA medium.

Then, a sample of a suitable size of wet tissue cut with sterile equipment was lifted up.

Then, after incubation in a constant temperature incubator, the flotation power was evaluated according to whether or not Halozone was generated.

At this time, the number of bacteria was counted using a plate culture method to confirm the decrease of bacteria and the death rate after 3 and 7 days of inoculation.

As a result of the control culture to confirm the concentration of the inoculum, it was confirmed that the bacterial mixture was inoculated with 7.2 * 10 ⁷ CFU / mL and A. niger 3 * 10 ⁵ CFU / mL.

In addition, it was judged that the bacterium was killed on the 7th day of inoculation, and that the bacteria and fungi inoculated were killed. The results of the measurement of the buoyant force of the wet tissue having a cooling sensation according to these experiments are shown in Table 2 below.

product name Inoculum Bacterial mixture Fungi (A. niger) Wet tissue with refreshing feeling Day 3 Day 7 Day 3 Day 7 0 (dead) 0 (dead) 300 0 (dead)

&Lt; Experimental Example 2: Measurement of heavy metal detection of liquid composition &

Whether or not the detection of the heavy metal of the wet tissue having a refreshing feeling manufactured according to Production Example 2 was measured and measured by the Korea Institute of Construction & Living Environment Test. At this time, the test method (1) is based on the safety standard for self-check safety [Wet Wipes (Wet tissue)] Annex 49 Notice No. 2012-0170 of the Technical Standards Agency. According to these experiments, the results of measurement of the detection of heavy metals in a wet tissue having a refreshing sensation are shown in Table 3 below.

Test Items unit Reference value Test Methods Test result Formaldehyde mg / kg Less than 20 (One) Not detected (detection limit 20) lead mg / kg Less than 20 (One) Not detected (detection limit 1) cadmium mg / kg Less than 20 (One) Not detected (detection limit 1) arsenic mg / kg Less than 20 (One) Not detected (detection limit 1) chrome mg / kg Less than 20 (One) Not detected (detection limit 1) Mercury mg / kg Less than 20 (One) Not detected (detection limit 1) Methanol mg / kg Less than 20 (One) Not detected (detection limit 1) benzene mg / kg Less than 20 (One) Not detected (detection limit 1) toluene mg / kg Less than 20 (One) Not detected (detection limit 1) Xylene mg / kg Less than 20 (One) Not detected (detection limit 1) Number of bacteria mg / kg Below 100 (One) <10 Fungus number mg / kg Below 100 (One) <10 Benzisothiazolinone mg / kg 15 or less (One) Not detected (detection limit 5) Chloromethyl isothiazolinone mg / kg 15 or less (One) Not detected (detection limit 15) Methyl isothiazolinone mg / kg Below 100 (One) Not detected (detection limit 5) Phenoxyethanol mg / kg 10000 or less (One) Not detected (detection limit 15) Triticale mg / kg 1000 or less (One) Not detected (detection limit 5) Parabens methylparaben mg / kg 4000 or less (One) Not detected (detection limit 5) Parabens ethylparaben mg / kg 4000 or less (One) Not detected (detection limit 5) Parabens Propylparaben mg / kg 4000 or less (One) Not detected (detection limit 5) Benzoized liquid seed mg / kg Below 5000 (One) Not detected (detection limit 20) Benzyl alcohol mg / kg 10000 or less (One) Not detected (detection limit 20) Benzalkonium chloride mg / kg Below 500 (One) Not detected (detection limit 7.5) Fluorescent brightener - Not to be detected (One) Not detected

&Lt; Experimental Example 3: Human Body Application Test of Liquid Composition >

In order to evaluate the safety of a wet tissue having a refreshing sensation manufactured according to Manufacturing Example 2 for human application, a safety evaluation using a skin patch was requested and conducted by ELID, a research institute.

At this time, the evaluation criteria are listed in the regulations of the Food and Drug Administration's functional cosmetics examination (No. 2013-190), PCPC (Personal Care Products Council) Safety Testing Guidelines, and International Contact Dermatitis Research Group (ICDRG) Followed by the evaluation criteria presented.

Here, the safety evaluation method by the skin patch is as follows.

First, 30 healthy male and female adults between 20 and 55 years old were selected and tested.

Then, the sample was applied (applied) to the back region of the test subject and removed after 24 hours.

Then, the test area from which the patch was removed was observed after 30 minutes, 24 hours, and 48 hours.

Then, the stimulation was judged according to the PCPC Safety Testing Guidelines and ICDRG standards.

Here, the patch test is a kind of induction test commonly used to attach an antigen known to cause allergic contact dermatitis to the skin. It is widely used as a diagnostic method for allergic contact dermatitis. And the progress of the dermatitis, it is possible to estimate the causative substance of contact dermatitis. However, the most excellent diagnostic method for identifying the causative agent is the patch test.

The evaluation criteria of the International Association of Contact Dermatological Research (ICDRG) are shown in Table 4 below.

sign score Criteria for Skin Response Remarks - 0 No stimulation ± 0.5 Irritation Faint or barely detectable mild erythema + One Light stimulus The border is clear but weak erythema, edema and palsy ++ 2 Moderate irritation Clear erythema, papules and small follicles +++ 3 River stimulation Severe erythema and alveoli, scab formation

The evaluation result of the skin patch test is shown in Table 5 below.

Grade mean score Unstimulated (1) 0.0 ~ 0.75 Unstable (2) 0.76 to 1.50 Light stimulus (3) 1.51 to 2.50 Medium Stimulus (4) 2.51 to 4.00 Stimulants stimulating (5) 4.01 ~

According to these experiments, the safety evaluation result of the skin patch of a wet tissue having a cooling sensation is shown in Table 6 below.

number Name of sample Number of reactants Reactivity Average skin response 30 min 24 hr 48hr #One Wet tissue with refreshing feeling 0 0.0 0.0 0.0 0.00

<Specific Review>

As in Experimental Example 1, as a result of the measurement of the flotation force of the wet tissue having a cooling sensation according to the present invention, it was confirmed that the bacteria died in the 7th day of inoculation, and the halozone, which does not grow mold on the wet tissue, Therefore, it can be confirmed that the wet tissue having a refreshing sensation according to the present invention has an antifungal effect against bacteria and fungi.

As in Experimental Example 2, it was found that heavy metals and fluorescent whitening agents of all kinds were not detected at all in the test results of the detection of heavy metals in a wet tissue having a refreshing feeling according to the present invention.

Accordingly, it can be confirmed that the wet tissue having a refreshing sensation according to the present invention does not contain harmful components to the human body and can be safely used without skin irritation.

As in Experimental Example 3, as a result of the safety evaluation by the skin patch of a wet tissue having a refreshing sensation according to the present invention, the reaction was observed in all test subjects after 30 minutes, 24 hours and 48 hours after removal of the patch I can confirm that I could not. That is, since the average skin reactivity corresponds to 0.00, it can be confirmed that the skin irritation determination is made.

Therefore, it can be confirmed that the wet tissue having the refreshing feeling according to the present invention belongs to the non-stimulated product group.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims It can be understood that

Claims (10)

Liquid compositions comprising purified water, ethanol, menthol and trehalose; And a wet tissue fabric,
The moisture absorptivity of the liquid composition on the wet tissue is 70 to 80 percent,
The liquid composition may contain,
An antiseptic comprising an electrification of 0.536 to 0.804 percent by weight of Cetylpyridinium Chloride and 0.66 to 0.99 percent by weight of sodium dehydroacetate; &Lt; / RTI &gt;
Wet tissue with refreshing feeling.
The method according to claim 1,
The liquid composition may contain,
72.644 to 88.787 percent by weight (%) of said purified water;
8 to 12 weight percent (%) of said ethanol;
1.316 to 1.974 weight percent (%) of said menthol; And
Wherein said trehalose comprises from 0.956 to 1.434 percent by weight of said trehalose.
Wet tissue with refreshing feeling.
delete The method according to claim 1,
The liquid composition may contain,
Cupressus funebris powder, Bacillus, rice bran extract, soybean extract, atlantica bark extract, Scotch pine cone extract, And Asian plantain extract. &Lt; RTI ID = 0.0 &gt; 21. &lt; / RTI &
Wet tissue with refreshing feeling.
5. The method of claim 4,
Wherein the skin conditioning composition comprises:
Characterized in that it comprises any one or more of 0.624 to 0.936 weight percent (%) of the white flour, bacillus, rice bran extract, soybean extract, Atlas cedar bark extract,
Wet tissue with refreshing feeling.
The method according to claim 1,
The liquid composition may contain,
Characterized in that it further comprises at least one fragrance ingredient corresponding to 0.44 to 0.66 percent by weight (Fragrance Ingredient).
Wet tissue with refreshing feeling.
delete delete The method according to claim 1,
The liquid composition may contain,
Characterized in that it further comprises a solubilizing agent comprising 0.4 to 0.6 percent by weight of polyethylene glycol.
Wet tissue with refreshing feeling.
delete