KR102609952B1 - Ample type cosmetic composition improving skin elasticity, Manufacturing method of the same and Ample type comsmetics improving skin elasticity - Google Patents

Abstract

The present invention relates to a cosmetic using extracts derived from natural materials. More specifically, it relates to a cosmetic using natural plant extracts such as finger root rhizome extract, which has excellent skin whitening and skin wrinkle improvement effects, and a method for manufacturing the same. and cosmetics using the same.

Description

Ample type cosmetic composition improving skin elasticity, manufacturing method of the same and Ample type cosmetics improving ampoule formulation skin elasticity improvement cosmetic using skin elasticity ingredient material of naturally derived plant finger root, its manufacturing method and ampoule formulation skin elasticity improving cosmetics skin elasticity}

The present invention relates to an ampoule-type skin elasticity cosmetic with excellent skin whitening and skin wrinkle improvement effects using finger root rhizome extract and various natural plant-derived extracts, a method for manufacturing the same, and an ampoule-type cosmetic using the same.

Recently, as a result of research on physiological mechanisms through dermatology and research on extracting and refining active ingredients from natural products as a new material with high efficacy, cosmetics that were simply used to protect the skin have expanded their efficacy to the scope of skin improvement. Demand for functional cosmetics is rapidly increasing.

In particular, among functional cosmetics, the production amount and market growth rate for multi-functional cosmetics that have two or more functions in one product (for example, including both wrinkle-improving and whitening functions) are significantly higher than those of single-functional cosmetics. However, most cosmetics currently on the market do not fully satisfy these requirements.

Fingerroot (Scientific name: Boesenbergia pandurata) is a plant in the ginger family that grows in Southeast Asia and is used not only in traditional Asian dishes such as pickles, curries, and drinks, but also as a tonic for childbirth, stomach pain, diarrhea, flatulence, indigestion, and ulcer treatment. It has been used for various purposes, including as a leukocyte preventive agent and as a cost supplement.

Finger root contains flavonoids that inhibit Helicobacter pylori bacteria infection, including indigestion and gastritis, and pinostrobin, which has a cell-protective effect, increases gastric mucus and reduces the area of gastric ulcer formation. It is also effective in treating peptic ulcers. In addition, it contains ingredients such as flavonoid derivatives, pinocembrin, pinostrobin, ilpinetin, tadamonin, pandurata, and panduratin A, which can inhibit the growth of cancer cells, especially pandurata. The ingredient inhibits the growth of cancer cells, and panduratin A is known to have a preventive effect on prostate cancer and colon cancer.

According to recent literature, Panduratin, a functional ingredient extracted from the roots of ginger plants, especially finger roots, is a light yellow-brown powder extracted from the roots of finger roots, and was designated as a functional ingredient for health functional foods by the Ministry of Food and Drug Safety in 2013. It has been recognized as having a physiological activity level of 2.

Panduratin is a light yellow-brown powder extracted from the roots of a plant called fingerroot. It was recognized by the Ministry of Food and Drug Safety as a functional ingredient for health functional foods in 2013 (grade 2 bioactive function), and it protects skin health from skin damage caused by ultraviolet rays. It is known to contribute to reducing body fat by activating 'AMPK (AMP-activated protein kinase)', a sensor protein for maintaining energy homeostasis in the body.

However, panduratin, one of the components of finger root extract, is highly toxic, so caution is required when using it, and there are limitations in applying it as a functional cosmetic.

Korean Patent No. 10-2288066 (announcement date 2021.08.11) Korean Patent Publication No. 10-2021-0021747 (publication date 2021.03.02)

As the market demand for multi-functional cosmetics using natural ingredients increases, the present inventors developed a method to remove the toxicity of panduratin, a finger root extract that has the disadvantage of being slightly toxic but is effective in maintaining skin homeostasis. , By using the finger root rhizome extract from which toxicity has been removed, the beneficial ingredients of the finger root rhizome extract are applied as cosmetics and mixed with other natural plant-derived extracts to provide excellent skin whitening, anti-wrinkle, and moisturizing properties. The aim is to provide highly functional cosmetics that can improve skin elasticity and skin condition, a manufacturing method thereof, and cosmetics using the same.

In order to solve the above-described problem, the ampoule type cosmetic for improving skin elasticity of the present invention includes a first liquid, a second liquid, a third liquid, a fourth liquid, and a fifth liquid, and the first liquid contains a viscosity modifier and It contains purified water, and the second liquid includes xanthan gum; and a moisturizing agent containing glycerin and butylene glycol, wherein the third liquid contains methylglucet-20, niacinamide, glycereth-26, sodium hyaluronate, allantoin, and hydrolyzed collagen (Hydrolyzed Collagen) and adenosine; a skin conditioning agent containing one or more selected from among; humectant; and a solvent; wherein the fourth liquid contains an emulsifier, fragrance and solvent, and the fifth liquid includes a functional extract including a pearl extract and a natural plant complex extract; Lactobacillus fermentation lysate; and collagen synthesis promoters.

In addition, the present invention relates to a method of producing the ampoule type cosmetic for improving skin elasticity, wherein purified water and a viscosity modifier are added to the reactor, and then stirred for 30 to 50 minutes at 3000 to 4000 rpm to prepare the first liquid. Step 1; Step 2 of adding the second liquid to the reactor where the first liquid was prepared and then stirring for 20 to 40 minutes at 2000 to 3000 rpm; Step 3 of adding the third liquid to the reactor that performed step 2 and then stirring for 20 to 40 minutes at 1500 to 2500 rpm; Step 4 of adding the fourth liquid to the reactor that performed step 3 and then stirring for 20 to 40 minutes at 1500 to 2500 rpm; Step 5 of adding a pH adjuster to the reactor that performed step 4 and then stirring for 20 to 40 minutes at 2000 to 3000 rpm; and step 6 of adding the fifth liquid to the reactor in which step 5 was performed and then stirring for 20 to 40 minutes at 1500 to 2500 rpm.

In addition, step 7 of filtering and defoaming the mixed solution obtained from step 6; And it can be manufactured by further performing step 8 of maturing the mixed solution obtained from step 7.

In addition, the present invention relates to cosmetics for improving skin elasticity using cosmetics prepared by the method described above, and can provide cosmetics in an ampoule formulation.

The cosmetic of the present invention does not cause skin trouble, has excellent moisturizing properties, has excellent skin whitening and skin wrinkle improvement effects, and can be used to provide highly functional cosmetics derived from natural materials.

Figure 1 shows the cytotoxicity measurement results of the finger root rhizome extract for each solvent conducted in Experimental Example 1.
Figure 2 shows the cell protection measurement results of finger root rhizome extract for each solvent conducted in Experimental Example 2.
Figure 3 shows the results of measuring the cell protection effect of finger root rhizome extract for each solvent conducted in Experimental Example 2.
Figure 4 shows the cytotoxicity measurement results for each fraction of the ethanol extract of finger root rhizome performed in Experimental Example 3.
Figure 5 shows the results of measuring the cell protection effect for each fraction of the ethanol extract of finger root rhizome performed in Experimental Example 4.
Figures 6 and 7 show the results of measuring the cytotoxicity and cytoprotective effects of the water fraction of the ethanol extract of finger root rhizomes conducted in Experimental Example 4.
Figure 8 shows the results of evaluating the effect of intracellular collagen on the water fraction of the ethanol extract of finger root rhizome performed in Experimental Example 5.
Figure 9 shows the results of evaluating the intracellular antioxidant effect of the water fraction of the ethanol extract of finger root rhizomes conducted in Experimental Example 6.

“Skin improvement” as used in the detailed description of the present invention means any action or effect that suppresses or delays skin damage or disease symptoms, improves disease symptoms, or improves the health status of the skin.

“Extract” used in the detailed description of the present invention refers to an active ingredient isolated from a natural product, that is, a substance showing the desired activity. The extract can be obtained through an extraction process using water, an organic solvent, or a mixed solvent thereof, and includes the extract's dry powder or any form formulated using it. In addition, the extract includes fractions of the extract that have undergone the extraction process.

Unless otherwise defined in the detailed description of the present invention, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. there is. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and should not be interpreted in an ideal or excessively formal sense unless explicitly defined in the present application. No.

Hereinafter, the present invention will be described in more detail through the ampoule type skin elasticity improvement cosmetic of the present invention and the method of manufacturing the same.

The cosmetic of the present invention includes 7 to 12 parts by weight of the second liquid, 12 to 16 parts by weight of the third liquid, 0.2 to 1.0 parts by weight of the fourth liquid, and 13 to 16 parts by weight of the fifth liquid, based on 100 parts by weight of the first liquid. and preferably, based on 100 parts by weight of the first liquid, 7 to 11 parts by weight of the second liquid, 13 to 15.5 parts by weight of the third liquid, 0.25 to 0.8 parts by weight of the fourth liquid, and 13.5 to 15.5 parts by weight of the fifth liquid. And, more preferably, based on 100 parts by weight of the first liquid, 7.5 to 10.0 parts by weight of the second liquid, 13.2 to 15.0 parts by weight of the third liquid, 0.25 to 0.50 parts by weight of the fourth liquid, and 14.0 to 14.0 parts by weight of the fifth liquid. It may contain 15.2 parts by weight.

The first liquid serves as a solvent for cosmetics and contains 0.10 to 0.25% by weight of a viscosity modifier and the remaining amount of purified water, and preferably may contain 0.10 to 0.18% by weight of the viscosity modifier and the remaining amount of purified water. At this time, if the viscosity modifier content exceeds 0.25% by weight, the initial viscosity may become too high, so the components of the second to fourth liquids may not be properly dissolved in the first liquid, and the miscibility of the composition may be reduced. And, as the viscosity modifier, a general viscosity modifier used in the cosmetics industry can be used. A preferred example is acrylate/(C _10-30 alkyl)acrylate cross polymer (acrylate/(/(C _10-30 alklyl)acrylate). cross polymer) can be used.

Next, among the cosmetic ingredients, the second liquid may include 0.5 to 1.5% by weight of xanthan gum and the remaining amount of a moisturizer based on the total weight of the second liquid, preferably 0.6 to 1.0% by weight of xanthan gum and the entire second liquid. The remainder of the weight may include moisturizer. In addition, the moisturizing agent may include one or more selected from glycerin and butylene glycol, and preferably may include glycerin and butylene glycol in a weight ratio of 1:0.5 to 1.5.

Next, among the cosmetic ingredients, the third liquid may include 77 to 85% by weight of a skin conditioning agent, 1.0 to 3.0% by weight of a humectant, and the remaining amount of solvent based on the total weight of the third liquid, preferably 77.5% by weight of the skin conditioning agent. It may contain ~ 83.0% by weight, 1.2 ~ 2.5% by weight of humectant, and the remaining amount of solvent, and more preferably may contain 78.2 ~ 81.0% by weight of skin conditioning agent, 1.3 ~ 2.2% by weight of humectant, and the remaining amount of solvent. there is.

Among the third liquid ingredients, the skin conditioning agents include Methyl Gluceth-20, Niacinamide, Glycereth-26, Sodium hyaluronate, Allantoin, It may contain one or more types selected from Hydrolyzed Collagen and Adenosine, preferably 50 to 80 parts by weight of niacinamide, and glycerol-20, based on 100 parts by weight of methylglucet-20. 26 30 to 60 parts by weight, 20 to 40 parts by weight of sodium hyaluronate, 5 to 20 parts by weight of allantoin, 10 to 30 parts by weight of hydrolyzed collagen, and 1 to 5 parts by weight of adenosine, more preferably. Based on 100 parts by weight of methylglucet-20, 60 to 72 parts by weight of niacinamide, 40 to 55 parts by weight of glycerol-26, 30 to 40 parts by weight of sodium hyaluronate, 6 to 15 parts by weight of allantoin, hydrol. It may contain 12 to 25 parts by weight of risen collagen and 1 to 3 parts by weight of adenosine.

Among the third liquid components, the wetting agent may include trehalose, and the solvent may include 1,2-hexane diol.

Next, the fourth liquid among the cosmetic ingredients may include 20 to 28% by weight of emulsifier, 5 to 20% by weight of fragrance, and the remaining amount of solvent, preferably 22 to 26% by weight of emulsifier and 5 to 15% by weight of fragrance. % and the remaining amount of solvent, and more preferably, 22.5 to 25.5% by weight of emulsifier, 5 to 12% by weight of fragrance, and the remaining amount of solvent.

Among the fourth liquid components, the emulsifier serves to increase the miscibility and emulsibility of the cosmetic composition, and includes PEG-60 hydrogenated castor oil, polysorbate 60, and stearic acid. and sorbitan sesquioleate, and may preferably include PEG-60 hydrogenated castor oil.

Among the fourth liquid components, it is recommended to use an ethanol aqueous solution as the solvent, and the type of fragrance may be any general fragrance used in the art as long as it does not cause skin trouble or impede the function of the cosmetic of the present invention.

Next, among the cosmetic ingredients, the fifth liquid may include 3.5 to 8.0% by weight of Lactobacillus fermentation lysate, 1.0 to 2.5% by weight of collagen synthesis accelerator, and the remaining amount of functional extract based on the total weight of the fifth liquid, preferably. It may include 3.8 to 6.5% by weight of Lactobacillus fermentation lysate, 1.1 to 2.0% by weight of collagen synthesis promoter, and the remaining amount of functional extract based on the total weight of the fifth liquid. At this time, among the fifth liquid components, the Lactobacillus fermentation lysate plays a role in promoting the ripening of the functional extract. If the content is less than 3.5% by weight, the functional extract does not ripen well and may cause skin problems. 8.0% by weight Excessive use is uneconomical.

In addition, the collagen synthesis promoter is absorbed into the skin and plays a role in promoting collagen synthesis in the skin, and RH-oligopeptide-1 can be used. In addition, if the collagen synthesis promoter content is less than 1.0% by weight, the amount used is too small, so the effect of promoting collagen synthesis in the skin is minimal, and the effect of improving skin wrinkles may be minimal, and if used in excess of 2.5% by weight, the miscibility of other compositions may be reduced. It is recommended to use it within the above range as it may interfere with it.

In addition, the functional extract includes pearl extract and natural plant complex extract, and the pearl extract and natural plant complex extract are used in a weight ratio of 1:3.5 to 5.0, and preferably, the pearl extract and natural plant complex extract are used in a weight ratio of 1:3.8 to 4.5. It can be included as .

The pearl of the pearl extract refers to a bead-shaped mass of secretion formed in the body of a shellfish. After crushing 100 g of pearls, 2 to 4 L of 60 to 80% by volume ethanol aqueous solution is used as a solvent for 10 to 16 hours. Step 1: reflux extraction with moderate heating; Step 2 of cold soaking the refluxed extract and then filtering it with filter paper to obtain a filtrate; It can be prepared by performing a process including three steps: concentrating the obtained filtrate through reduced pressure concentration and reduced pressure drying to obtain dried pearl powder.

In addition, the natural plant complex extract includes finger root rhizome extract (Boesenbergia Pandurata Rhizome Extract), Centella asiatica extract, Hippophae Rhamnoides Extract, pot marigold extract (Calendula Officinalis Extract), and green tea extract (Camellia Sinensis Leaf Extract). , Chamomilla Recutita Extract, Morus Alba Leaf Extract, Crataegus Pinnatifida Fruit Extract, Mentha Piperita (Peppermint) Extract, Hibiscus Sabdariffa Flower Extract) and rosemary extract (Rosmarinus Officinalis (Rosemary) Extract), preferably, based on 100 parts by weight of finger root rhizome extract, 5.0 to 10 parts by weight of Centella asiatica extract, 0.2 to 2.0 parts by weight of vitamin tree extract, and Port Marie. Gold extract 0.2 to 2.0 parts by weight, green tea extract 0.2 to 2.0 parts by weight, Matricaria extract 0.2 to 2.0 parts by weight, mulberry leaf extract 0.05 to 0.5 parts by weight, hawthorn fruit extract 0.05 to 0.5 parts by weight, peppermint extract 0.05 to 0.5. Parts by weight, 0.05 to 0.5 parts by weight of hibiscus flower extract and 0.05 to 0.5 parts by weight of rosemary extract, more preferably 5.0 to 9.0 parts by weight of centella asiatica extract, and vitamin tree extract, based on 100 parts by weight of finger root rhizome extract. 0.2 ~ 1.5 parts by weight, port marigold extract 0.2 ~ 1.5 parts by weight, green tea extract 0.2 ~ 1.5 parts by weight, matricaria extract 0.2 ~ 1.5 parts by weight, mulberry leaf extract 0.05 ~ 0.30 parts by weight, hawthorn fruit extract 0.05 ~ 0.30 It is recommended to include 0.05 to 0.30 parts by weight of peppermint extract, 0.05 to 0.30 parts by weight of hibiscus flower extract, and 0.05 to 0.30 parts by weight of rosemary extract.

The finger root rhizome extract is an extract from which toxicity has been removed, and it is better to use the finger root rhizome ethanol extract or the water fraction of the finger root rhizome ethanol extract, preferably the detoxified finger root rhizome ethanol extract. It is recommended to use the water fraction, which can be prepared through the following method.

The water fraction of the finger root rhizome ethanol extract is obtained by mixing finger root rhizome powder with an aqueous ethanol solution, stirring, and performing an extraction process at 15 to 30°C for 18 to 24 hours to produce an ethanol extract. Step 1 to obtain; Step 2: filtering the ethanol extract, adding distilled water, and concentrating in a vacuum to obtain a concentrate from which ethanol has been removed; and re-dissolving the concentrate in DMSO (Dimethyl sulfoxide), filtering it through a sterilizing filter, and then freeze-drying it.

And, in the water fraction production process, step 3 includes adding dichloroform to the concentrate, stirring it, leaving it to stand, and then removing the lower layer; step 3-1; And after removing the lower layer liquid, ethyl acetate is added, stirred and left to obtain a lower layer liquid, and then step 3-2 of freeze-drying the obtained product and powdering it.

Hereinafter, a method of manufacturing a skin elasticity cosmetic containing the first to fifth liquids described above will be described.

The cosmetic of the present invention includes the first step of preparing a first liquid by adding purified water and a viscosity modifier to a reactor and then performing stirring; Step 2 of adding the second liquid to the reactor in which the first liquid was prepared and then performing stirring; Step 3 of adding the third liquid to the reactor that performed step 2 and then performing stirring; Step 4 of adding the fourth liquid to the reactor that performed step 3 and then performing stirring; Step 5 of adding a pH adjuster to the reactor that performed step 4 and then performing stirring; It can be prepared by adding the fifth liquid to the reactor in which step 5 was performed and then performing step 6 of stirring.

In addition, after performing step 6, step 7 of filtering and degassing the mixed solution obtained from step 6; And it can also be manufactured by further performing a process including step 8 of maturing the mixed solution after performing step 7.

The stirring in step 1 is performed under the conditions of 60 to 80°C and a stirring speed of 3000 to 4000 rpm, preferably for 30 to 50 minutes, preferably for 35 to 45 minutes, under the conditions of 65 to 75°C and a stirring speed of 3200 to 3800 rpm. It is good to practice. At this time, if the temperature during stirring is less than 60℃ or the stirring speed is less than 3000rpm, there may be a problem of the viscosity modifier not completely dissolving in the purified water, which may prolong the stirring time, and if the temperature during stirring exceeds 80℃, it is uneconomical. , If the stirring speed exceeds 4000 rpm, foam may occur, so it is recommended to perform stirring under the above conditions.

The second stage of stirring is performed under the conditions of 45 to 60°C and a stirring speed of 2000 to 3000 rpm, preferably for 20 to 40 minutes, preferably for 20 to 35 minutes, under the conditions of 45 to 55°C and a stirring speed of 2200 to 2700 rpm. It is good to practice.

In addition, the third step of stirring is performed at 35 to 45°C and a stirring speed of 1500 to 2500 rpm, preferably for 20 to 40 minutes, preferably at 35 to 45°C and a stirring speed of 1800 to 2300 rpm. It is recommended to practice for minutes.

In addition, the stirring in the fourth step is performed at 35 to 45°C and a stirring speed of 1500 to 2500 rpm, preferably for 20 to 40 minutes, preferably at 35 to 45°C and a stirring speed of 1800 to 2300 rpm. It is recommended to practice for minutes.

In addition, the fifth stage of stirring is performed after adding the pH regulator, at a temperature of 15 to 30°C and a stirring speed of 2000 to 3000 rpm, preferably at a temperature of 20 to 30°C and a stirring speed of 2200 to 2700 rpm, for 20 to 40 minutes. Preferably it is performed for 20 to 35 minutes. In addition, arginine can be used as a preferred example of the pH adjuster, and it is appropriate to add 0.10 to 0.50 parts by weight, preferably 0.15 to 0.35 parts by weight, based on 100 parts by weight of the first liquid.

In addition, the 6th stage of stirring is performed under the conditions of 15 to 30°C and a stirring speed of 1500 to 2500 rpm, preferably for 20 to 40 minutes, preferably 20 to 35 minutes, under the conditions of 15 to 30°C and a stirring speed of 1800 to 2300 rpm. It is best to practice it for a while.

The skin elasticity improvement cosmetic of the present invention manufactured using the composition and method described above can be manufactured into any type of cosmetic product commonly manufactured in the art, and contains a dermatologically acceptable medium or base, so that it is commonly used in the field of dermatology. It can be formulated as an adjuvant for topical or systemic application.

Additionally, it can preferably be manufactured into an ampoule formulation cosmetics, and the ampoule formulation cosmetics may have a viscosity of 12,000 to 20,000 cP and a pH of 6.0 to 7.0.

Although the present invention has been described above with a focus on embodiments, this is only an example and does not limit the embodiments of the present invention, and those skilled in the art will be able to understand the essential characteristics of the present invention. It can be seen that various modifications and applications not exemplified above are possible without departing from the scope. For example, each component specifically shown in the embodiments of the present invention can be modified and implemented. And these variations and differences in application should be construed as being included in the scope of the present invention as defined in the appended claims.

[Example]

Preparation Example 1: Preparation of finger root rhizome extract

(1) After taking 5g of finger root rhizome powder, add 100ml of hexane, dichloroform, ethyl acetate, ethanol, methanol, and water for each solvent. and extracted at 25°C for 24 hours using a shaker to obtain each extract. Next, the obtained extract was filtered using a filter (Whatman No.2 filter paper), and then the extract for each solvent was concentrated in vacuum. The concentrate was re-dissolved in DMSO (dimethyl sulfoxide), filtered through a 0.22um sterilizing filter, and stored frozen at -20°C. Then, the water extract was filtered, lyophilized, and the powder was redissolved in 50% by volume DMSO and then filtered through a sterilizing filter for use.

Dichloroform, ethyl acetate, ethanol, and methanol solvent extracts were light yellow, and hexane extracts were colorless.

(2) Index component analysis

Finger root rhizome extract was aliquoted at the same concentration and prepared as an HLPC sample.

The content of panduratin A in the extract was analyzed by comparing the HPLC chromatogram of the extract by solvent with panduratin A, an indicator component of the finger root extract. The chemical structure of panduratin A is shown in Chemical Formula 1 below.

[Formula 1]

For the analysis method, panduratin A was dissolved in methanol and diluted stepwise to prepare the sample for quantitative purposes. The HPLC measurement conditions are shown in Table 1 below, and the validation of the analysis method is shown in Table 2 below.

item Analysis conditions Detector UV 280nm Column C ₁₈ (4.6mm ID × 250mm, 5μm) mobile phase
(Mobile phase) Time (minutes) A[DW](%) B[methanol](%) 5 40 60 10 0 100 15 0 100 25 40 60 30 40 60 Column temperature 55℃ injection volume 20μL flux 1mL/min

specificity Accurately weigh about 100 mg of Panduratin A, dissolve it in the diluent, and adjust to 100 mL to make a standard solution (approximately 1,000 mg/kg). Then, dilute this standard solution with the diluent to analyze the Panduratin A standard solution and the diluted solution without Panduratin A. The results were compared with the analysis results of the standard solution. linearity The anduratin A standard was analyzed at four concentrations, and linearity was evaluated by the coefficient of determination (r2). A standard solution was prepared by diluting the prepared standard solution (approximately 1,000 mg/kg) with MeOH. range In the validation test method, the concentration of the standard solution for which linearity is proven when creating the Panduratin A calibration curve is 1 mg/kg, 5 mg/kg, and 10 mg/kg, considering the accuracy test concentration and precision test concentration. , the concentration range was set at 50 mg/kg. Accuracy (recovery rate) A standard stock solution (1,000 mg/kg) of Panduratin A was added to MeOH without Panduratin A in a certain amount for each concentration. Then, MeOH was added and dispersed by ultrasonic waves for 15 minutes. After filtration (0.20㎕ syringe filter), it was used as the sample solution. The recovery rate was confirmed and evaluated after repeating the measurement three times independently for each concentration. Precision (reproducibility) Add 0.5 mL of Panduratin A standard stock solution (1 000 mg/kg) to MeOH without Panduratin A, then add 5 mL of 10% MeOH, disperse by ultrasonic wave for 15 minutes, volume up to 10 mL, and filter (0.20 ㎕ syringe). filter) and used as the sample solution. Panduratin A (concentration of 50 mg/kg)) was measured repeatedly 7 times and the system suitability was confirmed based on the relative standard deviation of the results. Detection limits and quantification limits Accurately weigh about 100 mg of Panduratin A standard, dissolve in the mobile phase, and dilute to 100 mL to make a standard stock solution (approximately 1,000 mg/kg). Then, dilute this standard stock solution in a diluent and measure the minimum standard solution concentration 10 times to determine the detection limit and The limit of quantification was calculated.

And, the results of measuring the panduratin A content in the extract by solvent are shown in Table 3 below.

extraction solvent Retention time Area Concentration (ppm) Panduratin A 1 ppm 29.377 6133 1.348 Panduratin A 5 ppm 29.379 40774 4.660 Panduratin A 10 ppm 29.380 96179 9.956 Panduratin A 50 ppm 29.365 515463 50.036 hexane 29.197 3364 1.088 Ethyl acetate 29.346 34382 4.042 dichloroform 29.336 7134 1.437 methanol 29.364 30281 3.663 ethanol 29.362 34237 4.030

Looking at the measurement results in Table 3, the indicator panduratin A was detected in all extraction solvents at 29.3 minutes, and the highest concentrations of panduratin A were detected in the following order: ethyl acetate (4.042), ethanol (4.030), and methanol (3.663). It was confirmed that it contained

Experimental Example 1: Toxicity test of finger root rhizome extract

Toxicity tests were performed on each of the nucleic acid extract (H), dichloroform extract (Ch), ethyl acetate extract (EA), ethanol extract (Et), methanol extract (Me), and water extract prepared in Preparation Example 1. The results are shown in Figure 1 A to C. AsA used in the test is a positive control and is indicated as ascorbic acid (Vit.C).

Extracts using nucleic acid (hexane, H), dichloroform (Ch), ethyl acetate (EA), ethanol (Et), methanol (Me), and water (W) as solvents, respectively. As a result of treating skin cells Hs68 at concentrations of 0.1, 0.5, 1, and 5μg/ml, all extracts except the water extract showed high toxicity at 5μg/ml.

Experimental Example 2: Cell protection effect and collagenase production inhibition experiment of finger root rhizome extract against UVB stimulation

(1) Cell protective effect

In order to measure the cytoprotective effect against UVB stimulation, the results of an experiment measuring the cytoprotective effect against UVB stimulation of the finger root rhizome extract for each solvent were treated at the same concentration as when measuring cytotoxicity, and the results are shown in Figures 2A to C. shown in As shown in Figure 2, the cell protection effect was not clearly observed in the remaining samples except ethanol and methanol.

In addition, Figures A and B of Figure 3 are graphs showing the results of experiments at concentrations of 0.5 and 1 μg/ml to compare the cell protection effect against UVB stimulation of finger root rhizome extracts for each solvent. As shown in Figure 3, as a result of the cell protection effect test against UVB stimulation, the ethanol extract showed the best effect, and the protective effect significantly increased at 0.5 μg/ml compared to 1 μg/ml, and the finger root The stem ethanol extract showed the highest cell protection effect against UVB.

(2) Collagenase production inhibition experiment

A test was performed on the efficacy of the ethanol extract of the finger root rhizome to inhibit collagenase production (Pro-collagen synthesis) at 0.5 μg/ml, 1.0 μg/ml, and 2.5 μg/ml, and the results are shown in Table 4 below. The positive control group was 100ng/ml of TFG-beta1, and the control group was the untreated group with ethane water extract.

division MMP-1 production (% compared to control) control group 100% Positive control group 63.5% Ethanol extract 0.5μg/ml 75.6% Ethanol extract 1.0μg/ml 70.8% Ethanol extract 2.5μg/ml 63.3%

Through the collagenase production inhibition experiment shown in Table 4 above, it was confirmed that the ethanol extract of finger root rhizome has an effect of inhibiting collagenase activity, and through this, has an effect of improving skin wrinkles.

Preparation Example 2: Preparation of fractions of finger root rhizome extract

Take 100g of finger root rhizome powder, add 1L of ethanol, and extract at 25°C for 24 hours using a shaker. An ethanol extract of finger root rhizome was obtained by filtering using a filter (Whatman No.2 filter paper). 100ml of 1L of finger root rhizome ethanol extract was taken and 200ml of distilled water was added.

The mixture of finger root rhizome ethanol extract and distilled water was concentrated under vacuum, all ethanol was removed, and then transferred to a separatory funnel. A total of 2L of dichloroform was added here, shaken sufficiently, left to stand, and then separated, and the lower layer was collected and concentrated.

After dichloroform fractionation, a total of 1L of ethyl acetate was added, shaken sufficiently, left to stand, separated, and the supernatant was collected and concentrated. Ethyl acetate was removed, and the remaining water layer was freeze-dried to form a powder. Both the concentrate and powder were re-dissolved in DMSO, then filtered through a 0.22um sterilizing filter, and stored at -20°C until analysis. The ethanol fraction, dichloroform fraction, ethyl acetate fraction, and water fraction for the ethanol extract of finger root rhizome were analyzed. were obtained respectively.

Experimental Example 3: Cytotoxicity test of ethanol extracted fraction of finger root rhizome

Cytotoxicity was performed on the ethanol-extracted fraction obtained in Preparation Example 2, and cell culture and cytotoxicity experiments were as follows.

Hs68 cells, a human skin fibroblast cell line used in cytotoxicity experiments, were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). DMEM (Dulbecco's Modified Eagle's Medium) containing 10% FBS, penicillin (100 units/ml), and streptomycin (50 μg/ml) was used at 37°C, 5% carbon dioxide (CO ₂ ), and 95% humid air. Cultured in an incubator (Thermo Scientific, Tewksbury, MA, USA) controlled by (humid air). Hs68 cells were distributed in a 96-well plate at a concentration of 1.0 After sample treatment for 24 hours, 20uL of MTT (1 mg/ml) solution was added to each well and incubated for 2 hours. Finally, the supernatant was removed, and the blue formazan crystals generated from the viable cells were solubilized with DMSO and the absorbance at 550 nm was measured using a Microplate reader (BioTek, Inc., Winooski, VT, USA). When processing samples, the samples were diluted with medium so that the final concentration of DMSO was less than 0.1% (v/v).

To evaluate cytotoxicity according to the concentration of each fraction, hs68 cells were treated with 0.1, 0.5, 1, and 5 μg/ml. As a result of treatment according to concentration, as shown in Figure 4 A and B, all fractions except the water fraction showed cytotoxicity at 5 μg/ml.

Experimental Example 4: Cell protection effect test on UVB stimulation of ethanol-extracted fraction of finger root rhizome

Hs68 cells were distributed in a 96-well plate at a concentration ^of 1.0

Afterwards, UVB (30mJ/cm ² ) was irradiated using a UVB lamp (Sankyo Denki Lamps, GL20SE, Marine, Japan), and the intensity of ultraviolet rays was monitored using a UV LIGHT METER (LT Lutron, UV-340A, Taiwan). . All UVB irradiation was performed after applying a thin layer of PBS to cells attached to a 96-well plate. After irradiation, samples were diluted in FBS-free medium and treated with cells for 24 hours. The control group was conducted under the same conditions without UVB irradiation.

To evaluate the cell protection effect against UVB irradiation for each of the ethanol-extracted fractions, cells were treated with extracts from each solvent at concentrations of 0.1, 0.5, 1, and 5 μg/ml before and after UVB irradiation. As a result, as shown in Figure 5A and B, only the water fraction maintained the protective effect without toxicity up to 5 μg/ml.

The water fraction of the ethanol extract of finger root rhizome was selected to measure cytotoxicity and cytoprotective effect against UVB at different concentrations (1, 5, 25, 50 μg/ml), and the results are shown in Figure 6. Looking at A in Figure 6, which is the cytotoxicity test result, in the case of cytotoxicity, it did not show cytotoxicity up to 25μg/ml, but the cell viability decreased to 86% at 50μg/ml, which was judged to indicate toxicity. Meanwhile, in the case of the cell protection effect, as shown in B of Figure 6, which is the result of the cell protection experiment, the cell protection effect was shown at 5 μg/ml and 25 μg/ml.

The above test results showed a slight decrease in cell viability at 25 μg/ml of the water fraction of the ethanol extract of finger root rhizomes, so the highest concentration was set at 20 μg/ml to obtain ideal results that increase in a concentration-dependent manner when processing the sample. did.

As a result of measuring the cytotoxicity and cytoprotective effect against UVB of 1 to 20 μg/ml of the water fraction of the ethanol extract of finger root rhizome, no toxicity was observed up to 20 μg/ml (see A in Figure 7), and the cytotoxicity was reduced by UVB. Cell viability increased in a concentration-dependent manner (see B in Figure 7).

Experimental Example 5: Evaluation of intracellular collagen effect on water fraction of finger root rhizome ethanol extract

UVB irradiation reduces collagen production in cells. Therefore, the effect of the water fraction of finger root extract on the amount of collagen in cells was measured. The experimental method is as follows.

(1) Measurement of MMP-1, MMP-3, and soluble collagen production

MMP-1 and MMP-3, the synthesis of which is increased by UVB irradiation, were measured using an enzyme-linked immunosorbent human MMP-1, MMP-3 ELISA kit (Merck & Co. Inc., Whitehouse Station, NJ, USA). The production amounts of pro-MMP-1 and MMP-3 in the medium were measured using the assay method. The amount of soluble collagen produced after UVB irradiation was measured using the SircolTM soluble collagen assay kit. The medium in which the cells were cultured was collected, mixed with Sirco dye reagent, reacted, centrifuged, and then cold acid-salt washing reagent was added to the precipitate and mixed. After centrifuging the mixture, the precipitate was dissolved using an alkaline reagent, and the absorbance was measured at 555 nm, and the experimental results are shown in A of Figure 8.

Looking at A of Figure 8, UVB decreased the amount of collagen in cells, and the level of collagen increased in a concentration-dependent manner as the concentration increased when treating the water fraction from 1 μg/ml to 20 μg/ml.

At this time, UVB irradiation promotes collagen decomposition by increasing the production of MMP-1 (Matrix MetalloProtease), an enzyme that decomposes Type 1 collagen, the most abundant protein in the skin. Therefore, the effect of the water fraction of finger root extract on MMP-1 production was evaluated by the above-described method, and the results are shown in Figure 8B. Upon UVB irradiation, MMP-1 production increased, and when treating the water fraction, MMP-1 production decreased as the concentration increased from 1 μg/ml to 20 μg/ml.

In addition, since MMP-3 promotes the decomposition of collagen by activating MMP-1, the amount of MMP-3 produced was measured in the same manner as for MMP-1, and the experimental results are shown in Figure 8C. Upon UVB irradiation, MMP-3 production increased, and when treating the water fraction, MMP-3 production decreased as the concentration increased from 1 μg/ml to 20 μg/ml.

Experimental Example 6: Evaluation of intracellular antioxidant effect of water fraction of finger root rhizome ethanol extract

UVB irradiation promotes the production of reactive oxygen species (ROS) within cells. This abnormal increase in reactive oxygen species causes oxidative stress and induces MMPs in dermal fibroblasts, thereby promoting skin aging. Therefore, the effect of the water fraction of finger root rhizome extract on ROS production was evaluated. The experimental method is as follows.

(1) Measurement of ROS production

To measure the production of reactive oxygen species (ROS), Hs68 cells were pretreated with samples for 24 hours, washed with PBS, and then cells were irradiated with UVB (30mJ/cm2). After UVB irradiation, the cells were treated with samples for 30 minutes and then stained with 25uM DCFH-DA. The fluorescence intensity corresponding to intracellular ROS production was measured with a fluorescent spectrophotometer (Perkin-Elmer, Norwalk, CT, USA) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm for 2 hours.

A fluorescence intensity measurement graph for measuring the production of ROS reactive oxygen species (ROS) according to the concentration of 1 to 20 μg/ml of the water fraction of the finger root ethanol extract is shown in Figure 9.

Looking at Figure 9, it was confirmed that UVB irradiation increased the production of ROS in cells, and that treatment of the water fraction reduced ROS production in a concentration-dependent manner.

Example 1: Preparation of ampoule-type skin elasticity improving cosmetics (cosmetics)

Purified water and acrylate/C _10-30 alkyl acrylate crosspolymer (Carbopol) were added to the reactor. After adding 0.13% by weight of ETD-2020) and 99.87% by weight of purified water, the first solution was prepared by stirring at 3,500 rpm at 70°C for 40 minutes.

After preparing a second liquid containing xanthan gum and a humectant, 8.13 parts by weight of the second liquid relative to 100 parts by weight of the first liquid was added to the reactor in which the first liquid was prepared, and then stirred for 30 minutes at a speed of 2,500 rpm at 50°C. was carried out. At this time, the composition of the second liquid is shown in Table 5 below.

2nd liquid ingredient weight% moisturizer glycerin 49.535 Butylene glycol 49.535 xanthan gum 0.83

Next, after preparing a third liquid containing a skin conditioning agent, a humectant, and a solvent, 14.17 parts by weight of the third liquid based on 100 parts by weight of the first liquid was added to the reactor in which the second liquid was mixed, and then heated at 40°C. Stirring was performed at 2,000 rpm for 30 minutes. At this time, the compositions of the third and fourth liquids are shown in Table 6 below.

Next, after preparing the fourth liquid containing an emulsifier, flavor, and solvent, 0.40 parts by weight of the third liquid based on 100 parts by weight of the first liquid was added to the reactor mixed with the third liquid, and then heated at 2,000 rpm at 40°C. Stirring was performed at high speed for 30 minutes. At this time, the composition of the fourth liquid is shown in Table 6 below.

third liquid ingredient weight% skin conditioning agent Methylgluceth-20 28.46 Niacinamide 18.98 Glycereth-26 14.23 Sodium Hyaluronate 9.48 allantoin 2.85 Hydrolyzed Collagen 4.74 Adenosine 0.38 humectant Trehalose 1.90 menstruum 1,2-hexanediol 18.98 total 100 4th liquid ingredient weight% emulsifier PEG-60 Hydrogenated Castor Oil 23.33 Spices Trehalose 10.00 menstruum Ethanol aqueous solution with a concentration of 70% by volume 66.67 total 100

Next, after lowering the temperature of the reactor in which the fourth liquid was mixed to 25 to 28°C, arginine, a pH adjuster, was added in an amount of 0.134 parts by weight based on 100 parts by weight of the first liquid, and then reacted at 2,500°C at 25 to 28°C. Stirring was performed at rpm speed for 30 minutes.

Next, after preparing the fifth liquid containing a functional extract including pearl extract and natural plant complex extract, Lactobacillus fermentation lysate, collagen synthesis promoter, and fragrance, 100 weight of the first liquid was added to the reactor mixed with arginine. 14.49 parts by weight of the fifth liquid was added to the reactor mixed with the pH adjuster, and then stirred at 2,000 rpm for 30 minutes at 25 to 28°C. At this time, the composition of the fifth liquid is shown in Table 7 below.

4th liquid ingredient weight% functional extract pearl extract 18.55 Natural plant complex extract 75.42 Lactobacillus fermentation lysate 4.64 Collagen synthesis promoter RH-Oligopeptide-1 1.39 total 100

At this time, the pearl extract was obtained by grinding 100 g of pearls, extracting under reflux for 12 hours using 3L of 70% by volume ethanol aqueous solution as a solvent, followed by cold soaking, and filtering through filter paper with a penetration size of 1.2㎛ to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure and dried under reduced pressure, then dried and pulverized.

In addition, the finger root rhizome extract is the water fraction of the ethanol extract, and the water fraction of the finger root rhizome ethanol extract prepared through the following process was used.

Take 100g of finger root rhizome powder, add 1L of ethanol, and extract at 25°C for 24 hours using a shaker. Next, an ethanol extract of finger root rhizome was obtained by filtering using a filter (Whatman No.2 filter paper). 100ml of 1L of finger root rhizome ethanol extract was taken and 200ml of distilled water was added. Next, the mixture of finger root rhizome ethanol extract and distilled water was concentrated in a vacuum, all ethanol was removed, and then transferred to a separatory funnel. A total of 2L of dichloroform was added here, shaken sufficiently, left to stand, and then separated, and the lower layer was collected and concentrated. Next, after the dichloroform fractionation, a total of 1L of ethyl acetate was added, shaken sufficiently, left to stand, the supernatant was separated, and the lower layer was freeze-dried to obtain a water fraction, which was then freeze-dried to obtain an ethanol extract of finger root rhizome. A dry powder of the water fraction was obtained.

In addition, the composition ratio of the natural plant complex extract is shown in Table 8 below, and other plant extracts other than the finger root rhizome extract were purchased commercially.

Simple plant complex extract weight part Finger root rhizome extract 100 Centella asiatica extract 6.67 vitamin tree extract 0.27 Port Marigold Extract 0.27 green tea extract 0.27 Matricaria Extract 0.27 Mulberry leaf extract 0.13 Hawthorn fruit extract 0.13 Peppermint Extract 0.13 Hibiscus flower extract 0.13 Rosemary Extract 0.13

Next, the solution prepared by mixing and stirring the fifth liquid was filtered and degassed, and then aged to finally prepare a cosmetic. The manufactured cosmetic was in an ampoule formulation, and had a viscosity of 14,000 to 16,000 cP and a pH of about 6.4 to 6.6 at 25°C.

Comparative Example 1: Preparation of skin elasticity cosmetic

Disclosed in Korean Patent No. 10-2288066 are the first mixture (sulfur agent, emulsifier, flavoring agent), the second mixture (purified water, adenosine, niacinamide), and the third mixture (natural plant-derived extract, natural plant-derived fermentation filtrate, After preparing the conditioning agent, stabilizer, preservative, and metal ion sequestrant), skin elasticity cosmetics were prepared through the following process.

In Table 9 below, the conditioning agents of the third mixture are Acetyl Hexapeptide-8, Copper Tripeptide-1, sh-Oligopeptide-10, and Butylene Glyph. It was prepared by mixing Butylene Glycol, Propylene Glycol, Hyaluronic Acid, and Methyl Perfluoroisobutyl Ether in equal weight ratios. .

After the first mixture was added to the first container, dispersed, dissolved, and mixed (step 11), the second mixture was dispersed, dissolved, and mixed (step 21). Next, the third mixture was dispersed, dissolved, and mixed. Mixed (step 31). The first mixture mixed in step 11, the second mixture mixed in step 21, and the third mixture mixed in step 31 were each transferred to an emulsifier and mixed and stirred in an emulsifier, etc. in step 32. Then, the mixture mixed and stirred in step 32 was filtered (step 33), vacuum degassed (step 34), transferred to a storage tank, and subjected to storage and aging (step 35) to prepare a skin elasticity cosmetic. The manufactured cosmetics were placed in a container and stored.

division ingredient Content (% by weight) First mixture
(10) emulsifier Polysorbate 80 4.0 softener Cyclopentasiloxane 7.5 flavoring agent combination spices 0.25 Second mixture
(20) Purified water 12.5 Adenosine 0.08 Niacinamide 2.5 Third mixture
(30) Finger Root Stem Extract 4.0 Centella asiatica extract 2.5 purslane extract 2.5 Mung Bean Extract 1.0 Eucalyptus Leaf Extract 1.0 Western Lion Extract 1.0 Mud Mushroom Extract 0.5 Matricaria Extract 0.5 Virginia Peach Blossom Extract 2.5 collagen extract 2.5 Lotus callus culture extract 1.2 Mulberry Bark Extract 1.2 Bambusa vulgaris extract 0.5 Bifida fermentation filtrate 7.5 Galactomyces fermentation filtrate 2.5 Yeast/Cordyceps ferment filtrate 2.5 conditioning agent 36.57 stabilizator Hydroxyethylcellulose 0.7 preservative 1,2-hexanediol 2.0 Metal ion sequestrant Disodium EDTA 0.5 Total weight% 100

Experimental Example 7: Measurement of wrinkle improvement effect around the eyes

The effect of improving wrinkles around the eyes was measured before and after using the cosmetics of Example 1 and Comparative Example 1. Wrinkles around the eyes were measured using PRIMOS (Phaseshift rapid in-vivo measurement of skin), and the evaluation results are shown in Table 10 below. Ra and R3z were selected as PRIMOS analysis variables, and the variable Ra (Arithmetic roughness average) is the arithmetic mean value of the peak of the roughness cross-section of wrinkles measured with PRIMOS. As the Ra value decreases, the depth of skin wrinkles decreases, resulting in wrinkles. This means improvement, and the unit is ㎛.

The variable R3z (Base roughness depth) is expressed as the arithmetic mean of five single roughness depths from R3z1 to R3z5. Single roughness depth refers to the vertical distance between the third highest cross section peak and the third deepest curve in the single evaluation distance (Ir) of the roughness cross section. The smaller the value of R3z, the lower the depth of wrinkles in the skin and the improvement in wrinkles. The unit is ㎛. The temporary improvement rate of wrinkles around the eyes was calculated according to Equation 1 below.

[Equation 1]

Improvement rate of eye wrinkles (%) = 100% - {(A-B)/B}*100%

In Equation 1, A is the measured value after using the product, and B is the measured value before using the product.

division Ra R3z Ra measurements Wrinkle improvement rate around the eyes (Ra) R3z measurements Improvement rate of wrinkles around the eyes (R3z) Before use 15.869 100% 42.237 100% Comparative Example 1 15.015 105.38% 40.250 104.70% Example 1 14.995 105.51% 40.129 104.99%

As a result of analyzing wrinkles around the eyes, both the cosmetics of Comparative Example 1 and Example 1 showed a statistically significant temporary improvement in wrinkles around the eyes after use compared to before use for both variables Ra and R3z (p<0.05), Comparative Example Example 1 showed a relatively better improvement rate of wrinkles around the eyes than Example 1.

Experimental Example 8: Measurement of skin elasticity improvement effect

The effect of improving skin elasticity before and after using the cosmetics of Example 1 and Comparative Example 1 was measured. The test area was measured three times using a Cutometer (Courage and Khazaka Electronic, Germany), and the average value was calculated using the three values. The measurement area was measured 3cm away from the corner of the eye. Cutometer applies negative pressure to the skin surface and measures the degree to which the skin is sucked into the probe using an optical system that can pass through the skin. The light used at this time is infrared, and the degree of elasticity is measured by the ratio of the intensity of light that decreases as it passes through the skin and the intensity of light that does not pass through the skin. R2 (A.U.) was selected as the Cutometer measurement variable for elasticity.

The variable R2 represents the ratio of the total contracted length of the skin to the maximum that the skin can stretch, and represents the overall elasticity, which is the re-deformability of the skin. The closer the value of this variable is to 1, the more elastic it is. The skin elasticity improvement rate was calculated according to Equation 1 below. Measurements were taken before use (0 weeks), after 2 weeks of use, and after 4 weeks of use.

No skin adverse events occurred due to the test product during the evaluation period, and the skin elasticity measurement results are shown in Table 11 below.

[Equation 2]

Skin elasticity improvement rate (%) = {(B-A)/B}*100%

In Equation 2, A is the R2 measurement value after using the product, and B is the R2 measurement value before using the product.

division 0 weeks
R2 measure 2 weeks later
R2 measure 4 weeks later
R2 measure 4 weeks later
Skin elasticity improvement rate (%) Comparative Example 1 0.7703 0.7767 0.7785 1.065 Example 1 0.7710 0.7783 0.7801 1.180

As a result of analyzing the wrinkles around the eyes, both the cosmetics of Comparative Example 1 and Example 1 showed an effect of improving skin elasticity after use compared to before use (p<0.05), and Example 1 was relatively better than Comparative Example 1. There was an improvement in wrinkles around the eyes.

Experimental Example 9: Evaluation of tyrosinase enzyme activity inhibition ability

The whitening activity effect of the cosmetic of Example 1 was evaluated through its ability to inhibit tyrosinase enzyme activity. Mix 1 mL of 0.1M sodium phosphate buffer (pH 6.9), cosmetic samples of Example 1 of various concentrations, and 30 ㎕ of mushroom tyrosinase (1 KU/mL), react at 37°C for 10 minutes, and add 1.5mM L-tyrosine 30 as a substrate. ㎕ was mixed and reacted at 25°C for 2 minutes. DOPA chrome produced in the reaction solution was measured at 475 nm, and the results are shown in Table 12. The absorbance of the control group was referred to as Ab _control , and the absorbance of the sample was referred to as Ab _sample . The absorbance was calculated according to Equation 3 below.

[Equation 3]

Tyrosinase enzyme activity inhibition rate (%) = (1-Ab _sample )/Ab _control *100%

Classification (cosmetic sample concentration, sample) Tyrosinase enzyme activity inhibition rate (%) 0.1mg/ml 9.7% 0.3mg/ml 18.8% 0.5mg/ml 20.5% 0.7mg/ml 29.5% 1.0mg/ml 35.2% 1.5mg/ml 37.0%

Through the evaluation of the tyrosinase enzyme activity inhibition rate in Table 12 above, it was confirmed that the cosmetic of the present invention has a skin whitening effect through inhibiting the activity of the tyrosinase enzyme.

Through the above examples and experimental examples, it was confirmed that the cosmetic of the present invention has various effects such as skin whitening and improvement of skin wrinkles, and it was confirmed that the cosmetic of the present invention can provide a variety of cosmetics for skin. .

Simple modifications or changes to the present invention can be easily implemented by those skilled in the art, and all such modifications or changes can be considered to be included in the scope of the present invention.

Claims (10)

Step 1 of preparing a first liquid by adding purified water and a viscosity modifier to the reactor and stirring for 30 to 50 minutes at 3000 to 4000 rpm;
Step 2 of adding the second liquid to the reactor where the first liquid was prepared and then stirring for 20 to 40 minutes at 2000 to 3000 rpm;
Step 3 of adding the third liquid to the reactor that performed step 2 and then stirring for 20 to 40 minutes at 1500 to 2500 rpm;
Step 4 of adding the fourth liquid to the reactor that performed step 3 and then stirring for 20 to 40 minutes at 1500 to 2500 rpm;
Step 5 of adding a pH adjuster to the reactor that performed step 4 and then stirring for 20 to 40 minutes at 2000 to 3000 rpm;
Step 6 of adding the fifth liquid to the reactor that performed step 5 and then stirring for 20 to 40 minutes at 1500 to 2500 rpm;
Step 7 of filtering and defoaming the mixed solution from step 6; and
Performing a process including step 8 of maturing the mixed solution after performing step 7,
The first liquid contains a viscosity modifier and purified water,
The second liquid includes xanthan gum; and a moisturizing agent containing glycerin and butylene glycol.
The third liquid is a skin conditioning agent containing one or more selected from methylgluceth-20, niacinamide, glycerol-26, sodium hyaluronate, allantoin, hydrolyzed collagen, and adenosine; humectant; and solvent;
The fourth liquid contains an emulsifier, fragrance, and solvent,
The pH adjuster includes arginine,
The fifth liquid is a functional extract including pearl extract and natural plant complex extract; Lactobacillus fermentation lysate; And a collagen synthesis promoter;
The natural plant complex extract includes finger root rhizome extract, centella asiatica extract, vitamin tree extract, port marigold extract, green tea extract, Matricaria extract, mulberry leaf extract, hawthorn fruit extract, peppermint extract, hibiscus flower extract and rosemary extract. Includes,
The collagen synthesis accelerator is a method of producing an ampoule-type skin elasticity improving cosmetic using the skin elasticity ingredient material of finger root, a naturally derived plant, characterized in that it contains RH-oligopeptide-1. According to claim 1, in the second step, 7 to 12 parts by weight of the second liquid is added to 100 parts by weight of the first liquid,
In the third step, 12 to 16 parts by weight of the third liquid is added to 100 parts by weight of the first liquid,
In step 4, 0.2 to 1.0 parts by weight of the fourth liquid is added to 100 parts by weight of the first liquid,
Step 6 is a method of manufacturing an ampoule-type skin elasticity improvement cosmetic using the skin elasticity component of finger root, a naturally derived plant, characterized in that 13 to 16 parts by weight of the fifth solution is added to 100 parts by weight of the first solution. . For 100 parts by weight of the first liquid, 7 to 12 parts by weight of the second liquid, 12 to 16 parts by weight of the third liquid, 0.2 to 1.0 parts by weight of the fourth liquid, and 13 to 16 parts by weight of the fifth liquid,
The first liquid contains a viscosity modifier and purified water,
The second liquid includes xanthan gum; and a moisturizing agent containing glycerin and butylene glycol.
The third liquid is a skin conditioning agent containing one or more selected from Methyl Gluceth-20, Niacinamide, Glycereth-26, Sodium Hyaluronate, Allantoin, Hydrolyzed Collagen, and Adenosine; humectant; and solvent;
The fourth liquid contains an emulsifier, fragrance, and solvent,
The fifth liquid is a functional extract including pearl extract and natural plant complex extract; Lactobacillus fermentation lysate; And a collagen synthesis promoter;
The natural plant complex extract includes finger root rhizome extract, centella asiatica extract, vitamin tree extract, port marigold extract, green tea extract, Matricaria extract, mulberry leaf extract, hawthorn fruit extract, peppermint extract, hibiscus flower extract and rosemary extract. Includes,
The collagen synthesis accelerator is an ampoule-type skin elasticity improvement cosmetic using skin elasticity ingredient material of finger root, a naturally derived plant, characterized in that it contains RH-oligopeptide-1. The method of claim 3, wherein the first liquid contains 0.10 to 0.25% by weight of a viscosity modifier and a remaining amount of purified water,
The second liquid contains 0.5 to 1.5% by weight of xanthan gum and the remaining amount of moisturizer,
The third liquid contains 77 to 85% by weight of a skin conditioning agent, 1.0 to 3.0% by weight of a humectant, and the remaining amount of solvent. Skin elasticity of an ampoule formulation using the skin elasticity ingredient material of finger root, a naturally derived plant. Improved cosmetics. The method of claim 3, wherein the fourth liquid contains 20 to 28% by weight of emulsifier, 5 to 20% by weight of fragrance, and the remaining amount of solvent,
The fifth liquid contains 3.5 to 8.0% by weight of Lactobacillus fermentation lysate, 1.0 to 2.5% by weight of collagen synthesis promoter, and the remaining amount of functional extract,
The functional extract is an ampoule-type skin elasticity improvement cosmetic using the skin elasticity ingredient of finger root, a naturally derived plant, characterized in that it contains pearl extract and natural plant complex extract at a weight ratio of 1:3.5 to 5.0. delete The method of claim 3, wherein the natural plant complex extract is 5.0 to 10 parts by weight of Centella asiatica extract, 0.2 to 2.0 parts by weight of vitamin tree extract, 0.2 to 2.0 parts by weight of port marigold extract, based on 100 parts by weight of finger root rhizome extract, Green tea extract 0.2 ~ 2.0 parts by weight, Matricaria extract 0.2 ~ 2.0 parts by weight, mulberry leaf extract 0.05 ~ 0.5 parts by weight, hawthorn fruit extract 0.05 ~ 0.5 parts by weight, peppermint extract 0.05 ~ 0.5 parts by weight, hibiscus flower extract 0.05 ~ An ampoule-type skin elasticity improvement cosmetic that contains 0.5 parts by weight and 0.05 to 0.5 parts by weight of rosemary extract and uses the skin elasticity ingredient of finger root, a naturally derived plant. The ampoule formulation using the skin elasticity component of the naturally derived plant finger root according to claim 3, wherein the finger root rhizome extract is an ethanol extract of the finger root rhizome or a water fraction of the ethanol extract of the finger root rhizome. Cosmetic for improving skin elasticity. An ampoule formulation skin elasticity improving cosmetic, characterized in that it contains any one of the cosmetics selected from claims 3 to 5, 7, and 8. The ampoule formulation skin elasticity improvement cosmetic according to claim 9, wherein the ampoule formulation has a viscosity of 12,000 to 20,000 cP and a pH of 6.0 to 7.0 at 25°C.

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