KR102032830B1 - Fuctional food improving skin condition comprising rice bran extract - Google Patents

Abstract

The present invention relates to a composition for improving skin containing rice bran extract as an active ingredient, the rice bran extract of the present invention by inhibiting the MAPK pathway to inhibit the expression of MMP-13 and COX-2 by ultraviolet irradiation to damage the skin by ultraviolet rays It can be usefully used as a material for health functional foods and oral cosmetics that provides skin moisturizing, skin exfoliation, skin wrinkle improvement, and skin photoaging.

Description

Functional food improving skin condition comprising rice bran extract}

The present invention relates to a composition for improving skin and a dietary supplement containing rice bran extract.

Recently, with the improvement of the quality of life and the desire for healthy and beautiful life, interest in skin beauty and health is expanding. Accordingly, various cosmetic functional cosmetics have been developed according to the purpose of maintaining healthy skin, and research for preventing, alleviating and improving skin wrinkle formation is being actively conducted. In addition, recently, cosmetic ingredients have a limit in supplying nutrients to the skin dermis, and as a change in the perception that the nutritional or functional ingredients can be supplied to the skin to increase skin beauty, the inner beauty food Excavations are also actively underway.

Skin aging is largely divided into endogenous aging due to physiological aging and photoaging caused by continuous ultraviolet radiation (UV) exposure. Repeated UV exposure results in increased expression of collagenase and induces denaturation and destruction of collagen fibers, reducing the elasticity of the skin and promoting the production of wrinkles. That is, the generation of reactive oxygen species (ROS) increases continuously in skin tissues exposed to ultraviolet rays, and is transmitted through a signaling system mediated by growth factor receptor (EGF-R), tumor necrosis factor (TNF) -receptor, etc. Promotes inflammatory cytokine production. Activation of these receptors consists of a continuous phosphorylation that activates downstream signaling mediators, including mitogenactivated protein kinase (MAPK), resulting in activator protein-1 (AP-1) and It activates transcription factors such as nuclear factor κB (NF-κB) to induce inflammatory reactions and promotes the activity of collagenase, such as matrix metalloproteinases (MMPs), thereby reducing skin elasticity and promoting wrinkle formation. AP-1 regulates the expression of numerous genes involved in cell growth and differentiation and strongly regulates the expression of some MMPs. Among the MMPs whose expression is controlled by AP-1, MMP-13 is known as collagenase 13, and is based on collagen of type 1 to 3 as a substrate.

On the other hand, rice is a valuable food resource, one of the world's three major grains, which half of the world's population uses as a staple food, and is a key national resource that cannot be replaced by other crops, especially in Asia. Rice that has been stripped of rice husk, which is not the outer shell of rice, is called brown rice, and rice that has been polished with rice and stripped of rice bran and rice eyes is called rice. In particular, rice bran is an excellent ingredient in terms of nutrition despite being a by-product of polished rice. Rice bran has been shown to have various effects such as anti-cancer, anti-oxidation, anti-inflammatory, anti-arteriosclerosis, cholesterol lowering, growth promotion, digestive function, and immuno-enhancing effect. It has not been revealed.

The present inventors conducted a study to find a material that is effective in improving wrinkles when ingested as a food from natural products with few side effects, and found that rice bran extract exhibits improved skin moisturization, wrinkle improvement and skin photoaging effect. The invention has been completed.

Korea Patent Publication No. 10-1367961

It is an object of the present invention to provide a food composition for skin improvement comprising rice bran extract as an active ingredient.

Another object of the present invention to provide a health functional food comprising the food composition for skin improvement.

Still another object of the present invention is to provide a method for preparing the food composition for skin improvement.

In order to achieve the above object, the present invention provides a food composition for skin improvement comprising the rice bran extract as an active ingredient.

In addition, the present invention provides a health functional food comprising the food composition for skin improvement.

In addition, the present invention is the step of extracting rice bran with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, concentrating the rice bran extract obtained in the step, the rice bran extract concentrate obtained in the step and polyoxyethylene sorbitol mono It provides a method for producing a food composition for skin improvement comprising the step of mixing laurate, casein sodium and tocopherol and spray drying the mixture obtained in the step.

The food composition containing the rice bran extract of the present invention as an active ingredient has improved skin moisture content, promotes collagen biosynthesis, inhibits collagen fiber damage, inhibits collagen fiber degradation, improves skin moisturization, improves skin elasticity, and is excellent in inhibiting thickening of the skin epidermal layer. It can be usefully used to improve skin wrinkles and skin photoaging.

In addition, since the rice bran extract of the present invention is a natural product, it is safe for the human body and has less side effects, and thus may be used in various materials such as health functional foods and oral cosmetics.

Figure 1 shows the weight gain (1a) and the water content (1b) of the skin tissue of the mice fed the experimental diet.
Figure 2 is a skin thickness (2a) and skin epidermal layer (2b) of the mouse fed the experimental diet.
Figure 3 shows the degree of wrinkle formation (3a) and the total wrinkle area (3b) of mice fed the experimental diet.
Figures 4a and 4b shows the effect of inhibiting the expression of COX-2 and MMP-13 of rice bran extract in the animal model of the experimental diet.
Figures 5a and 5b shows Western blot results showing the effect of rice bran extract on UVB-induced MAPK phosphorylation in animal models ingested experimental diet.

Rice bran extract has been reported to have anti-cancer, antioxidant, anti-inflammatory, anti-arteriosclerosis, cholesterol lowering, growth-promoting, digestive function, and immune-strengthening effects. No new activity has been reported specifically for skin health.

Under this background, the inventors orally administered rice bran extract to an animal model to find out the novel activity of rice bran extract related to skin health, and apply the composition containing rice bran extract as an active ingredient to health functional food, medicine, cosmetics, etc. In order to prepare the powder by various methods and conditions in order to find out the powder production conditions having oxidation prevention, proper flow to complete the present invention.

The composition provided by the present invention may be usefully used to improve skin moisturizing, skin elasticity improvement, skin wrinkles and skin photoaging by including rice bran extract as an active ingredient.

In the present invention, the rice bran extract may be extracted by using rice bran water, an organic solvent or a mixture thereof as an extraction solvent.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol may be used as the lower alcohol. Organic solvents include polar solvents such as acetic acid, dimethyl-formamide (DMFO) and dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, and decane. , Cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chloro Nonpolar solvents such as benzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, anneal, diethylamine, ether, carbon tetrachloride, and THF (Tetrahydrofuran) can also be used.

According to one embodiment of the invention, the rice bran extract may be a lower alcohol extract of rice bran, preferably may be an ethanol extract of rice bran.

On the other hand, the term included herein as an active ingredient means to include an amount sufficient to achieve the efficacy or activity of the rice bran extract. In one embodiment of the present invention, the rice bran extract or rice bran powder in the composition of the present invention is, for example, at least 1.0 mg / kg, preferably at least 100 mg / kg, more preferably at least 500 mg / kg, at least Included.

Since the rice bran extract is a natural product, even if it is excessively administered, there is no side effect to the human body, so that the upper limit of the amount of the rice bran extract or rice bran powder included in the composition of the present invention can be carried out by those skilled in the art.

The term extract used while referring to rice bran herein includes not only the crude extract obtained by treating the extraction solvent on the rice bran, but also the processed product of the rice bran extract. For example, rice bran extract may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

According to a preferred embodiment of the present invention, in the food composition for skin improvement of the present invention, the rice bran extract is prepared by extracting the rice bran with a solvent and then concentrated by spray drying in the application of rice bran extract to health functional food It is preferable at the point of improving suppression and workability.

In addition, according to a preferred embodiment of the present invention, in the food composition for skin improvement of the present invention, the rice bran extract is a rice bran extract powder prepared by mixing all or part of polyoxyethylene sorbitol monolaurate, casein sodium and tocopherol It is preferable from the viewpoint of suppressing rancidity and improving workability.

According to a preferred embodiment of the present invention, the skin improvement food composition of the present invention is characterized in that the oral cosmetic composition.

In the food composition for skin improvement of the present invention, the skin improvement is characterized by consisting of skin moisturizing, skin wrinkle improvement, skin photodamage improvement or skin erythema reduction.

As used herein, the term "improvement of skin wrinkles" means maintaining or enhancing the ability to be associated with wrinkles and elasticity of the skin. Collagen (collagen) and elastic fiber elastin (collagen) in the dermal layer of the skin is the main protein that plays a role in the skin elasticity, collagen biosynthesis is affected by the internal and external skin. Specifically, when skin cells are reduced in cell activity due to natural aging, a decrease in collagen fibers occurs, or free radicals generated by excessive irradiation of UV rays or stress as external factors cause the protein's thiol group (thi-SH). In response to inhibiting the enzyme activity or by increasing the expression of degradation enzymes, such as collagen, elastin, increase the wrinkles of the skin and decrease the elasticity to progress the skin aging.

In one embodiment of the present invention by feeding the experimental diet containing rice bran extract to the hairless mice with UV irradiation for 16 weeks, the skin moisture content of the experimental animal, wrinkles of the back skin tissue, changes in skin thickness, skin tissue MMP-13 Gene expression and the like were measured. As a result, it was confirmed that rice bran extract increased the skin moisture content (Fig. 1b), and also significantly reduced the thickness of the epidermal layer thickened by ultraviolet irradiation (Fig. 2a and 2b). In addition, the rice bran extract has the effect of improving the wrinkles of the back skin tissue of the experimental animal, significantly reducing the wrinkle area, depth and length to significantly reduce the formation of wrinkles (Figs. 3a and 3b), COX-2 and MMP Significantly reduced -13 gene expression increased collagen protein synthesis and inhibited the degradation of collagen fibers to effectively inhibit wrinkle formation by ultraviolet irradiation (Figs. 4a and 4b).

Therefore, the composition containing the rice bran extract of the present invention can be usefully used as a material for health functional foods and oral cosmetics, such as to improve the skin moisturizing, improve wrinkles, improve skin photoaging.

Accordingly, the present invention relates to a health functional food comprising a food composition for skin improvement.

In the present invention, the term "health functional food" refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functions for the human body. Here, 'functional' means to obtain a useful effect for health purposes such as nutrient control or physiological action on the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and the preparation can be prepared by adding raw materials and ingredients commonly added in the art. In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. Unlike the general medicine, the health functional food composition of the present invention has the advantage that there are no side effects that may occur when taking a long-term use of the drug as a raw material, and has excellent portability, improving skin moisturizing, enhancing skin elasticity, and improving skin wrinkles. And / or as an adjuvant to enhance skin photoaging improvement.

The food composition for skin improvement of the present invention may be prepared in any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings.

According to a preferred embodiment of the present invention, the food composition for skin improvement of the present invention comprises rice bran extract as an active ingredient in the form of powder, liquid, tablets, soft capsules, granules, tea bags, instant tea or drink Can be converted. The content of rice bran extract as an active ingredient can be suitably determined according to the purpose of use (prevention or improvement). In general, the amount of rice bran extract contained in the nutraceutical composition may be added to 0.1 to 90% by weight of the total food weight. However, in the case of prolonged intake for health and hygiene purposes or health control purposes, the amount may be below the above range. In addition, the health functional food composition according to the present invention, in addition to the rice bran extract within the range that does not impair the main effect for the purpose of the present invention, for example, vitamin C and other ingredients that can have a synergistic effect on the main effect It may also contain natural compounds such as compounds for improving wrinkles or green tea extracts, eoseongcho extract, licorice extract, lettuce extract, honggyeongcheon extract, golden extract, wild ginseng extract.

The dietary supplement composition formulated in the form as described above may be added to the food as it is or used with other foods or food ingredients, and may be appropriately used according to conventional methods. Examples of foods include drinks, meat, sausages, breads, biscuits, rice cakes, chocolates, candy, snacks, confectionery, pizza, ramen, dairy products including other noodles, gums, ice creams, various soups, beverages, alcoholic beverages and vitamin complexes. , Dairy products and dairy products, and all functional foods in the conventional sense.

When the health functional food composition of the present invention is a drink, it contains rice bran extract as an essential ingredient at the indicated ratio, and there are no special restrictions on other ingredients used for the manufacture of other drinks, and various flavoring agents such as ordinary drinks. Or natural carbohydrate or the like as an additional component. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As a flavoring agent other than the above-mentioned, a natural flavoring agent, a synthetic flavoring agent, etc. can be used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition, the nutraceutical composition of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the food composition of the present invention may contain a pulp for producing natural fruit juice and fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the rice bran extract of the present invention.

According to a preferred embodiment of the present invention, the health functional food of the present invention has the advantage of having an improved skin moisturizing effect by ingesting in the form of inner beauty (inner beauty) food. The inner beauty (inner beauty) is an inner beauty food, called cosmetics or beauty foods to eat, refers to a food that changes the physical constitution of the skin by absorbing a variety of ingredients that are good for the skin, and choose a cosmetic that fits the skin type Considering your skin condition and lifestyle, you can choose the inner beauty food that suits you. More preferably, when the cosmetic composition containing the cosmetic composition and the inner beauty food containing the compound are mixed, the skin whitening improvement effect is significantly higher than that of using only the cosmetics, so that the effect of improving the skin's moisturizing effect is improved. Have

The present invention also relates to quasi-drugs or pharmaceutical compositions having the effect of improving skin moisturizing, enhancing skin elasticity, improving skin wrinkles and / or improving skin photoaging, containing rice bran extract as an active ingredient.

When using the rice bran extract of the present invention as a medicine, it may further contain one or more active ingredients exhibiting the same or similar function. For example, it may include known skin wrinkle improving ingredients or elasticity enhancing ingredients. By including additional skin wrinkle improving component and elasticity enhancing component, the wrinkle improvement and elasticity enhancing effect of the composition of the present invention can be further enhanced. When the ingredient is added, skin safety, ease of formulation, and stability of the active ingredients may be considered. In one embodiment of the present invention, the pharmaceutical composition is a wrinkle improving component known in the art, any one selected from the group consisting of vitamin C, retinoic acid, TGF, protein from animal placenta, betulinic acid and chlorella extract The skin may further contain wrinkle improvement ingredients. Additional skin wrinkle improving ingredients may be included in 0.0001 to 10% by weight based on the total weight of the composition, the content range may be adjusted according to requirements such as collagen synthesis promoting activity, skin safety, ease of formulating rice bran extract, etc. Could be.

In addition, the pharmaceutical composition for moisturizing the skin of the present invention may further comprise a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers may contain various components such as buffers, sterile water for injection, general saline or phosphate buffered saline, sucrose, histidine, salts, polysorbates and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and may be administered in the form of a general pharmaceutical preparation, for example, in various dosage forms, orally and parenterally, during clinical administration. , Diluents or excipients such as extenders, binders, wetting agents, disintegrants, surfactants and the like. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient in the pharmaceutical composition of the present invention, for example, starch, calcium carbonate, Sucrose, lactose, gelatin, etc. may be mixed. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrologol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The pharmaceutical composition of the present invention may provide a desirable skin moisturizing improvement, skin elasticity improvement, skin wrinkle improvement or skin photoaging improvement effect when an effective amount of rice bran extract is included. In the present invention, the term 'effective amount' means an amount of a compound capable of inhibiting or inhibiting generation of wrinkles on the skin or alleviating wrinkles already produced. The effective amount of rice bran extract included in the composition of the present invention will vary depending on the form in which the composition is commercialized, the method in which the compound is applied to the skin, the time it stays on the skin, and the like. For example, when the composition is commercialized as a medicament for dermatological treatment due to wrinkle formation, elasticity reduction, and blemishes of the skin, rice bran extract may be contained at a higher concentration than when it is commercialized as a cosmetic product that is routinely applied to the skin. There will be.

The present invention also suppresses rancidity in order to apply the rice bran extract to health functional foods and oral cosmetics to effectively protect the active ingredient of rice bran, improve the flowability (flow) of the powder to improve rice bran extract powder It relates to a method for producing a food composition for skin improvement comprising.

According to a preferred embodiment of the present invention, the manufacturing method of the present invention, the step of extracting rice bran with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof; Concentrating the rice bran extract obtained in the step; Mixing the rice bran extract concentrate obtained in the step with polyoxyethylene sorbitol monolaurate, sodium casein and tocopherol; And spray drying the mixture obtained in the step.

According to a preferred embodiment of the present invention, in the manufacturing method of the present invention, the rice bran used in the step of extracting the rice bran can be produced a product that does not become rancid by extracting using raw materials within 3 days after milling. . Table 1 shows the acid value change of the rice bran raw material according to the storage days.

Item Control Storage days 1 day 2 days 3 days 5 days 7 days 15th Room temperature 13.5 18.1 20.9 23.2 33.8 45.8 71.3 cold storage 13.5 16.4 16.6 19.6 21.2 24.1 27.5 35 ℃ 13.5 20.2 23.5 26.4 42.5 58.2 90.5

According to a preferred embodiment of the present invention, in the manufacturing method of the present invention, the mixing step is polyoxyethylene sorbitol monolaurate, casein sodium in the concentrate of rice bran extract to improve the flowability of rice bran powder and prevent rancidity And tocopherol (natural antioxidant) is preferably added and mixed.

According to a preferred embodiment of the present invention, the mixing step of the manufacturing method of the present invention is 0.5 to 1.5 parts by weight of polyoxyethylene sorbitol monolaurate, 2 to 5 parts by weight of sodium casein and 100 parts by weight of rice bran extract concentrate and It is preferable to add 0.005 to 0.1 parts by weight of tocopherol and mix. If it is out of the above-described content range, the flowability of the powder may be sharply lowered.

Skin improvement food composition comprising the rice bran extract powder prepared by the production method of the present invention is to prevent rancidity, the content of the active ingredient is maintained for a long time and the flow of the powder is excellent, for the production of health functional foods and oral cosmetics, etc. It can be used in various ways.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Example 1 Preparation of Rice Bran Extract

Peeling the rice bran layer from the rice during the milling process causes the rancidity of the rice bran to rise rapidly due to the action of lipase. After 5 days, the degree of rancidity increased rapidly. Thus, rice bran ethanol extract was prepared from the rice bran within 3 days after the induction of rice bran as the raw material, and the extraction time was 2 to 16 hours, and extracted for 8 hours at the temperature of 40 ± 3 ° C. Rice bran extract has a high content of the active ingredient confirmed that the skin moisturizing effect is excellent to prepare a rice bran ethanol extract under the above conditions. Table 2 shows the yield and the amount of the active ingredient according to the extraction conditions of the rice bran ethanol extract.

Extraction time (hr.) 2 4 6 8 10 16 30 ℃ Yield (%) 12.6 16.6 19.2 20.1 20.1 20.3 Indicator substance (mg / g) 2.9 3.2 3.4 3.7 3.7 3.5 40 ℃ Yield (%) 17.7 22.1 24.4 25.1 25.1 26.3 Indicator substance (mg / g) 3.6 4.0 4.5 4.6 4.6 4.5 50 ℃ Yield (%) 18.8 23.1 25.5 26.7 26.7 27.5 Indicator substance (mg / g) 3.7 4.2 4.6 4.7 4.7 4.5

Example 2. Preparation of Rice Bran Extract Powder

In order to increase its utility as an inner beauty product, it needs to be prepared into a pill, capsule, or tablet formulation. Thus, rice bran extract powder having a flowability suitable for preparing the formulation was prepared. The rice bran extract prepared in Example 1 was filtered and concentrated using a vacuum pressure reducer to a temperature of 13 ± 2 Brix at a temperature of 65 ° C. or lower. 100 parts by weight of the concentrated rice bran extract concentrate was added to each of the additives as shown in Table 3 below, homogenized by stirring at 3000 rpm for 10 minutes, sterilized at a temperature of 80 ± 3 ° C. for 15 minutes, and using a spray drying method. The rice bran extract powder was prepared by drying under the conditions of an inlet temperature of 160 ° C., an outlet temperature of 90 ° C., and a flow rate of 8-10 L / h.

Tween 20 Casein sodium Tocopherol Example 2 1.2 3 0.01 Comparative Example 1 - - - Comparative Example 2 0.3 - - Comparative Example 3 0.8 - - Comparative Example 4 1.2 - 0.01 Comparative Example 5 1.7 - 0.01 Comparative Example 6 1.2 One 0.01 Comparative Example 7 - 3 0.01 Comparative Example 8 1.2 6 -

Experimental Example 1. Check the flowability of the rice bran extract powder

In order to determine the flowability of the powder to the rice bran extract powder of Example 2 and Comparative Examples 1 to 8, each sample was taken 30g and stirred at 100 rpm for 2 minutes to measure the cohesiveness and fluidity. The degree of cohesion was visually determined and evaluated based on the fact that there was no agglomeration of the powder particles (×), a slight agglomeration phenomenon (△), a moderate agglomeration phenomenon (○), and a high agglomeration phenomenon (◎). In addition, the fluidity (cm 3 / sec) was measured by passing the powder per unit time by measuring the time that the powder passes between the mark (30 cm) where the volume becomes 288 cm 3 between the upper and lower ends of the inner diameter 3.5 cm and the length 60 cm glass tubes. Obtaining the volume to evaluate the flowability of the powder, the results are shown in Table 4 below.

Cohesion liquidity Example 2 × 115 Comparative Example 1 ◎ 52 Comparative Example 2 ◎ 58 Comparative Example 3 ◎ 50 Comparative Example 4 ◎ 49 Comparative Example 5 ◎ 53 Comparative Example 6 ○ 71 Comparative Example 7 ○ 81 Comparative Example 8 X 105

Experimental Example 2. Changes in Acidity of Rice Bran Extract Powder

In order to determine the acid value change during storage for the rice bran extract powder of Example 2 and Comparative Examples 1 to 8, in order to measure the acid value of the rice bran 100g of rice bran and put in a container 300ml of extraction solvent (n-hexane) 1 The oil was extracted by shaking 2-3 times at room temperature for 2 hours, and extracted twice by the same method. When extraction was completed, sulfuric anhydride was added to the dried filter paper and the filtrate was concentrated under reduced pressure to obtain a fat or oil. Take about 1 to 3 g of the oil and fat solution, put it in an erlenmeyer flask, and dissolve 10 ml of ethanol-ether (1: 2) mixture. Titration, and the results are shown in Table 5.

Item storage duration Control 1 day 5 days 15th 30 days 60 days Example 2 16 16.2 16.8 17.1 17.9 18.5 Comparative Example 1 16 19.2 21.5 30.4 42.5 75.4 Comparative Example 2 16 18.0 19.2 26.8 39.2 62.5 Comparative Example 3 16 18.2 20.2 28.7 31.2 63.4 Comparative Example 4 16 17.5 19.2 30.2 39.8 55.4 Comparative Example 5 16 16.9 20.2 28.2 40.2 51.5 Comparative Example 6 16 17.8 20.2 22.2 28.5 32.5 Comparative Example 7 16 18.4 19.8 24.5 36.8 47.5 Comparative Example 8 16 18.1 22.2 30.6 41.2 54.6

Example 3. Breeding of test material, laboratory animals and UV irradiation

Rice bran extract, a test substance, was prepared and prepared by the method of Examples 1 to 2, and the dandelion extract, which received bioactive level 2 as a "skin moisturizing" function from the Ministry of Food and Drug Safety, was selected as a positive control group. Dandelion extract was added to 80g ethanol to 18g of dandelion powder (100% domestic, clear field Co., Ltd., Hongcheon, Gangwon-do) was extracted for 24 hours. The extract was concentrated using a rotary evaporator (SB-1200, EYELA, Tokyo, Japan), and powdered by lyophilization.

Six-week-old female hairless mice (SKH-1 hairless mice) used in this example were purchased from Central Experimental Animal Co., Ltd. (Seoul, Korea) and used for 1 week. Experimental animals were divided into 5 groups and 10 animals were assigned to each group and used for the experiment. The experimental group was divided into the normal control group (normal), the ultraviolet group (UV), and the UV group irradiated with rice bran extract (0.1% diet or 0.5% diet) or dandelion extract. Feed and water were freely ingested during the breeding period, and the temperature was maintained at 23 ± 2 ℃, the humidity was maintained at 40-60%, and the photoperiod and dark cycle were adjusted to 12 hours daily. The rice bran extract ingestion group supplied the diet prepared by adding 0.1% or 0.5% of rice bran extract prepared by the method of Preparation Example for 16 weeks, and the dandelion extract intake group was added to the diet containing 0.5% of the dandelion extract prepared by the above method. Breeding weekly. The diet was fed with water at 2 pm daily, and the dietary intake was measured daily. During the breeding period, ultraviolet B (UVB) was irradiated to the back of the hairless mice three times a week, and the amount of ultraviolet radiation was 1 MED (1 MED = 0.5 J / cm 2, MED; minimal erythema dose) for the first week. 2 MED, 3 MED at 3 weeks, 4 MED from 4 to 16 weeks. Body weights were measured weekly during breeding, and photographs of the back skin were performed. On the last day of the experiment, the animals were fasted for 6 hours and blood and back skin tissues were collected under anesthesia.

Experimental Example 3. Changes in moisture content of skin tissues of UV-radiated hairless mice (Fig. 1, a, b)

In order to confirm the skin moisturizing effect of rice bran extract, the moisture content of skin such as hairless mice was evaluated by skin capacitance measurement (Skin-O-Mat, Cosmomed, Germany) and 20 ℃ (± 2 ℃) and 50% (±) for 1 hour. 5%) was kept at humidity. For further experiments, if the standard deviation for five or six consecutive measurements on the day before euthanasia was less than 0.1, the average of the last five measurements was taken as the water value of each animal.

Ultraviolet irradiation, rice bran extract and dandelion extract intake did not significantly affect the weight and dietary intake of hairless mice (FIG. 1A). In the + UVB control group irradiated with ultraviolet rays for 16 weeks, the water content of the skin tissue was significantly decreased while the erythema index was significantly increased compared to the normal group (-UVB) which was not irradiated with ultraviolet rays (FIGS. 1B and 1C). In the group of rice bran extract, the moisture content was significantly increased by 20% compared to the + UVB control group, even though UV was irradiated at the same intensity, especially in the group of rice bran extract (0.5%). It was high (Fig. 1b).

Experimental Example 4. Changes in skin thickness of UV-radiated hairless mice (Figs. 2A and 2B)

As photoaging progresses due to ultraviolet rays, the formation of the stratum corneum increases to protect the dermal layer of the skin, thereby thickening the skin. Therefore, the thickness of the skin epidermal layer increased by ultraviolet irradiation means that the skin damage caused by photoaging is large. J Molecular mechanism of skin ageing Mech Ageing Dev 123: 801-810, 2002). To confirm the skin damage caused by photoaging, the back skin tissues of hairless mice were extracted and fixed in 10% neutral formalin on the last day of experimental breeding, and stained with hematoxylin and eosin (H & E), followed by fluorescence microscopy (Nikon Eclipse Ti-). S, Tokyo, Japan) and images were analyzed with Metamorph (Molecular Devices, Danville, PA) software.

As a result, the + UVB control group showed a significant increase in skin thickness compared to the normal group (-UVB), which was not irradiated with UV light, and the group treated with rice bran extract showed a 42.6% decrease in skin thickness compared to the + UVB control group. (FIG. 2A). The skin epidermal layer thickness of hairless mice by H & E staining of skin tissues also showed that the + UVB control group thickened the skin epidermal layer compared to the normal group (-UVB) without UV irradiation. It was confirmed that the thickness of the epidermal layer thickened by ultraviolet irradiation was significantly reduced (FIG. 2B).

Experimental Example 5 Changes in Skin Wrinkle Production of Ultraviolet Irradiated Hairless Mice (FIGS. 3A and 3B)

To evaluate the effect of intake of rice bran extract on the formation of skin wrinkles, photographs of the back skin of experimental animals were taken. The skin of hairless mice subjected to ultraviolet irradiation for 16 weeks was molded with silicone rubber to measure the extent of wrinkle formation. A disk with a hole was attached to the back of the hairless mouse, and a silicone polymer (Silfo, Flexico Developments LDT, England) reagent was mixed, spreaded thinly on the back of the hairless mouse, completely dried, and carefully removed to prepare a replica plate. . The simulated plate was commissioned by Daegu Technopark (Daegu, Korea) and the skin wrinkle curves were evaluated using a skin wrinkle measuring device (Visioline VL650, Courage-Khazaka Electronic). Analyze using w CMOS camera.

The + UVB control group, which was repeatedly exposed to UVB for 16 weeks, was visually observed to have an abnormal increase in fine wrinkle formation along with a number of coarse and deeply creased wrinkles compared to the normal group (-UVB), which was not irradiated with UVB. In the rice bran extract intake group, the thickness and depth of the wrinkles were significantly reduced compared to the + UVB control group. Furthermore, the rice bran extract intake group showed an improvement similar to the skin condition observed in the -UVB group that was not irradiated with ultraviolet rays. It was confirmed (FIGS. 3A and B). The antiwrinkle effect of the rice bran extract was superior to the antiwrinkle effect observed in the same concentration of dandelion extract.

Experimental Example 6. Confirmation of the inhibitory effect of COX-2 expression by ultraviolet light of rice bran extract (a, b of Figure 4)

UV irradiation increases collagen degrading enzyme MMP-13 (collagenase) in the skin tissue, which mediates skin aging. In addition, expression of COX-2 induced by ultraviolet irradiation is involved in the inflammatory response. To investigate the effect of rice bran extract on the expression of COX-2 and MMP-13 by UV light, skin tissues of hairless mice were placed in a 2 mL microcentrifuge tube containing lysis buffer and stainless steel beads. TissueLyser II (Qiagen, Valencia, CA) , USA), and homogenized twice at 20 Hz for 2 minutes. Tissue homogenates were centrifuged at 12,000 rpm for 20 minutes. The protein was centrifuged and the supernatant was taken to measure the protein concentration using a DC Protein Assay kit (Bio-Rad Laboratories). Skin tissue extracts were separated by SDS-PAGE electrophoresis. After transferring it to a nylon membrane, the desired protein was isolated using an antibody. The fluorescence microscope (Nikon Eclipse Ti-S, Tokyo, Japan) was used to visualize epidermal thickness and MMP-13 and COX-2 expression, and images were analyzed with Metamorph (Molecular Devices, Danville, PA) software.

As a result, as can be seen in Figure 4a, the + UVB control is increased in the expression of COX-2 and MMP-13 by ultraviolet irradiation, and this increased COX-2 and MMP-13 expression is concentration-dependent in the rice bran extract intake group It was confirmed that the decrease, which was the same in tissue staining (Fig. 4b).

Experimental Example 7. Confirmation of the mechanism of suppression of MMP-13 and COX-2 expression (FIG. 5 a, b)

MAPK signals are known to play a central role in UV-induced MMP-13 and COX-2 expression. Thus, to determine whether rice bran extract blocks the UV-induced MAPK signal cascade in mouse skin tissues to reduce MMP-13 and COX-2 production, the JNK1 / 2, ERK1 / 2 and p38 MAPK proteins The effect of rice bran extract on phosphorylation was examined.

Total JNK1 / 2, ERK1 / 2 and p38 MAPK protein phosphorylation levels were determined by Western blot analysis, as shown in FIGS. 5A and 5B. That is, ERK1 / 2, JNK1 / 2, and p38 MAPK protein phosphorylation was induced in the + UVB control group, whereas UVB-induced ERK1 / 2, JNK1 / 2, and p38 MAPK protein phosphorylation were decreased in the rice bran extract group. Thus, it suggests that rice bran extract significantly inhibits UVB-induced MMP-1, COX-2 expression through inhibition of the ERK1 / 2, JNK1 / 2 and p38 MAPK pathways.

Preparation Example 1 Preparation of Health Functional Food

With the following composition, a dietary supplement containing rice bran extract was prepared.

1-1. Preparation of health functional food

Rice bran extract 1000 mg, vitamin mixture amount, vitamin A acetate 1.0 mg, vitamin E 0.13 mg, vitamin B1 0.15 mg, vitamin B2 0.5 mg, vitamin B6 0.2 μg, vitamin B12 10 mg, vitamin C 10 μg, biotin 1.7 mg, nicotinic acid 50 µg amide, folic acid 0.5 mg calcium pantothenate, mineral mixture, ferrous sulfate 1.75 mg, zinc oxide 0.82 mg magnesium carbonate 25.3 mg, potassium monophosphate 15 mg, potassium diphosphate 55 mg, potassium citrate 90 mg, Calcium Carbonate 100 mg, Magnesium Chloride 24.8 mg

1-2. Manufacture of healthy drinks

Rice bran extract 1000 mg, citric acid 1000 mg, oligosaccharide 100 g, plum concentrate 2 g, taurine 1 g, purified water

1-3. Manufacture of tablets

Rice bran extract 1000 mg, corn starch 100 mg, lactose 100 mg, magnesium stearate 2 mg

1-4. Preparation of Capsule

Rice bran extract 1000 mg, corn starch 100 mg, lactose 100 mg, magnesium stearate 2 mg

1-5. Manufacture of rings

1 g of rice bran extract, 1.5 g of lactose, 1 g of glycerin, 0.5 g of xylitol

1-6. Preparation of Granules

Rice bran extract 1000 mg, soy extract 50 mg, glucose 200 mg, starch 600 mg

Preparation Example 2 Preparation of Skin Ointment

Rice bran extract 0.5%, diethyl sebacate 8.0% by weight, light lead 5.0% by weight, polyoxyethylene oleyl ether phosphate 6.0% by weight, appropriate amount of sodium benzoate

Preparation Example 3 Preparation of Cosmetics

3-1. Preparation of Flexible Lotion

Rice bran extract 0.1%, ethanol 10.0%, polylauric acid polyoxyethylene sorbitan 1.0%, paraoxybenzoic acid methyl 0.2%, glycerin 5.0%, 1,3-butylene glycol 6.0%, fragrance amount Pigment amount

3-2. Manufacture of nutritional cream

Rice bran extract 0.2% by weight, stearic acid 15.0% by weight, cetanol 1.0% by weight, potassium hydroxide 0.7% by weight, glycerin 5.0% by weight, propylene glycol 3.0% by weight, preservative amount, fragrance amount, balance purified water

3-3. Manufacture of pack

Rice bran extract 0.05%, glycerin 5.0%, propylene glycol 4.0%, polyvinyl alcohol 15.0%, ethanol 8.0%, polyoxyethylene oleyl ethyl 1.0%, paraoxybenzoic acid methyl 0.2%, pigment Incense

Claims (10)

delete delete delete delete delete delete delete delete Extracting rice bran with ethanol at 40 ° C. for 8 hours;
Concentrating the rice bran extract obtained in the above step to 13 ± 2 Brix at a temperature of less than 65 ℃;
Mixing 1.2 parts by weight of Tween 20, 3 parts by weight of sodium casein, and 0.01 parts by weight of tocopherol based on 100 parts by weight of the rice bran extract concentrate obtained in the step; And
Sterilizing the mixture obtained in the above step at 80 ± 3 ℃ for 15 minutes, spray drying at the inlet temperature 160 ℃, outlet temperature 90 ℃, flow rate 8-10 L / h conditions of the food composition for improving skin wrinkles Manufacturing method. A food composition for improving skin wrinkles prepared by the method of claim 9.

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