KR20190053309A - Fuctional food improving skin condition comprising rice bran extract - Google Patents

Abstract

The present invention relates to a composition for improving skin containing a rice bran extract as an active component. The rice bran extract of the present invention suppresses a MAPK pathway to inhibit expression of MMP-13 and COX-2 by ultraviolet irradiation, thereby being able to be usefully utilized as materials for health functional foods, oral cosmetics and the like that can provide effects of skin moisturization, skin exfoliation, skin wrinkle alleviation, and skin photoaging alleviation against skin damage due to ultraviolet rays.

Description

Technical Field [0001] The present invention relates to a health functional food for improving skin containing rice bran extract,

The present invention relates to a skin improving composition containing a rice bran extract and a health functional food.

Recently, interest in skin beauty and health has been increasing as the quality of life and the desire for a healthy and beautiful life have increased. Various cosmetic functional cosmetics have been developed for the purpose of maintaining a healthy skin, and researches for prevention of skin wrinkle formation, mitigation and improvement have been actively carried out. In addition, recently, cosmetic ingredients reach skin dermis and there is a limitation in supplying nutrients. As a result of the change in perception that ingestion of nutrients or functional ingredients to the skin can provide a skin cosmetic enhancement effect, Research to excavate materials is also being actively pursued.

Skin aging is largely divided into endogenous aging by physiological aging and photoaging caused by continuous exposure to ultraviolet radiation (UV). Repeated exposure to ultraviolet light increases the expression of collagenase and induces denaturation and destruction of collagen fibers, thereby reducing skin elasticity and promoting the production of wrinkles. In other words, the development of reactive oxygen species (ROS) is increased in skin tissue exposed to ultraviolet rays continuously, and the signal transduction system mediated by growth factor receptor (EGF-R), tumor necrosis factor (TNF) Promoting the production of inflammatory cytokines. Activation of these receptors results in continuous phosphorylation, which activates proteins that mediate downstream signaling, including mitogen activated protein kinase (MAPK), resulting in activator protein-1 (AP-1) and Activation of transcription factors such as nuclear factor κB (NF-κB) induces inflammatory responses and promotes the activity of collagenases such as matrix metalloproteinases (MMPs), thereby reducing skin elasticity and promoting wrinkle formation. AP-1 regulates the expression of a number of genes involved in cell growth and differentiation and strongly regulates the expression of some MMPs. Among the MMPs whose expression is regulated by AP-1, MMP-13 is known as collagenase 13, and collagen of type 1 to 3 is used as a substrate.

On the other hand, rice is a valuable food resource, one of the world's top three grains, which is half of the world's population, and is a key national resource that can not be confronted with other crops, especially in Asia. Rice, which is rice husk, which is the surface of rice, is called brown rice, rice rice with rice husk and rice husk is removed and rice is called white rice. Especially, rice bran is a very good material for nutrition though it is a by-product of Jeongbaekmi. Rice bran has been shown various effects such as anticancer, antioxidant, anti-inflammation, anti-arteriosclerosis, cholesterol lowering, growth promoting action, digestive function promotion, immunity enhancing action, etc. However, the relationship between intake of rice bran and suppression of photoaging It has not been revealed.

The inventors of the present invention have conducted research to find a material effective for improving wrinkles when ingested as a food from a natural substance having a low side effect and found that rice bran extract has the effect of improving skin moisturization, Thereby completing the invention.

Korean Patent Registration No. 10-1367961

It is an object of the present invention to provide a skin composition for improving skin comprising an extract of rice bran as an active ingredient.

Another object of the present invention is to provide a health functional food comprising the food composition for skin improvement.

It is still another object of the present invention to provide a method for producing the skin composition for improving skin.

In order to achieve the above object, the present invention provides a skin composition for improving skin comprising an extract of rice bran as an active ingredient.

Further, the present invention provides a health functional food comprising the food composition for skin improvement.

The present invention also relates to a method for producing a rice bran extract, comprising the steps of extracting rice bran with water, an alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, concentrating the rice bran extract obtained in the above step, Mixing sodium laurate, sodium caseinate, and tocopherol; and spray drying the mixture obtained in the above step.

The food composition containing the rice bran extract of the present invention as an active ingredient is excellent in skin moisture content, promoting collagen biosynthesis, inhibiting collagen fiber damage, inhibiting collagen fiber decomposition, inhibiting the thickening of the skin surface layer, And can be usefully used for skin wrinkle improvement and skin photoaging improvement.

In addition, since the rice bran extract of the present invention is a natural product, it is safe for the human body and has few side effects, so that it can be widely used as a material for health functional foods and oral cosmetics.

Fig. 1 shows the body weight gain (1a) and the water content (1b) of the skin tissue of the mice ingested with the experimental diet.
Fig. 2 is the skin thickness 2a and the skin surface layer photograph 2b of the mouse that ingested the experimental diet.
Fig. 3 shows the degree of wrinkle formation (3a) and total wrinkle area (3b) of mice consuming the experimental diet.
4A and 4B show the inhibitory effect of rice bran extract on the expression of COX-2 and MMP-13 in an animal model in which the experimental diet was consumed.
Figures 5a and 5b show Western blot results showing the effect of rice bran extract on UVB-induced MAPK phosphorylation in animal models fed the experimental diets.

Rice bran extract has been reported to have anticancer, antioxidant, anti-inflammatory, anti-arteriosclerosis, cholesterol lowering, growth promoting action, promoting digestive function and immunity enhancing action, There has been no report on the new activity relating to skin health through < RTI ID = 0.0 >

Under these circumstances, the inventors of the present invention not only confirmed the novel activity of rice bran extract related to skin health by oral administration of rice bran extract to an animal model, but also applied a composition containing an extract of rice bran as an active ingredient to health functional foods, medicines and cosmetics The present inventors have completed the present invention by confirming the conditions for producing powders having various flow rates and preventing oxidation.

The composition of the present invention can be effectively utilized for improving skin moisturization, improving skin elasticity, improving skin wrinkles and improving skin photoaging by incorporating rice bran extract as an active ingredient.

In the present invention, the rice bran extract may be obtained by extracting rice bran using water, an organic solvent or a mixture thereof as an extraction solvent.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, as the lower alcohol, methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol can be used. The organic solvent may be a polar solvent such as acetic acid, dimethyl-formamide (DMFO) or dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, decane 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chloro A nonpolar solvent such as benzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF (tetrahydrofuran) may be used.

According to an embodiment of the present invention, the rice bran extract may be a low alcohol extract of rice bran, preferably an ethanol extract of rice bran.

In the present specification, it is meant that the term encompassed by the term active ingredient includes an amount sufficient to achieve the efficacy or activity of the rice bran extract. In one embodiment of the present invention, the rice bran extract or the rice bran powder in the composition of the present invention has a concentration of 1.0 mg / kg or more, preferably 100 mg / kg or more, more preferably 500 mg / kg or more .

Since the rice bran extract has no adverse effect on the human body even when administered in an excessive amount as a natural product, the quantitative upper limit of the rice bran extract or the rice bran powder contained in the composition of the present invention can be selected by a person skilled in the art within a suitable range.

The term extract used in reference to rice bran in the present specification includes not only the crude extract obtained by treating the rice bran with the extraction solvent but also the processed rice bran extract. For example, the rice bran extract may be prepared in powder form by an additional process such as vacuum distillation and lyophilization or spray drying.

According to a preferred embodiment of the present invention, in the food composition for skin improvement of the present invention, the rice bran extract is prepared by extracting rice bran with a solvent, concentrating it, and spray drying the rice bran extract to a health functional food, It is preferable from the viewpoint of suppression and enhancement of workability.

According to a preferred embodiment of the present invention, in the food composition for skin improvement of the present invention, the rice bran extract is a rice bran extract powder prepared by mixing all or a part of polyoxyethylene sorbitol monolaurate, casein sodium and tocopherol Is preferable in terms of inhibiting rancidity and improving workability.

According to a preferred embodiment of the present invention, the skin-improving food composition of the present invention is an oral cosmetic composition.

In the food composition for skin improvement of the present invention, the skin is improved by moisturizing the skin, improving the wrinkles of the skin, improving the skin photodamage, or reducing the skin erythema.

The term " skin wrinkle improvement " in the present invention means to maintain or enhance the ability of skin to be related to wrinkles and elasticity. Collagen (collagen) and elastin (elastin), which are the collagen fibers of the skin dermis layer, play a key role in maintaining skin elasticity. Collagen biosynthesis is affected by internal and external skin. Specifically, the skin cells are reduced in cellular activity due to natural aging, and collagen fibers are decreased. When extreme ultraviolet rays are irradiated as an external factor, or when active oxygen generated by stress or the like causes thiol (-SH) To increase the expression of collagen and elastase, thereby increasing the wrinkles of the skin and reducing the elasticity, thereby aging the skin.

In one embodiment of the present invention, an experimental diet containing rice bran extract and ultraviolet light irradiation was fed to hairless mice for 16 weeks, and skin moisture content, wrinkles, skin thickness change, Gene expression and the like were measured. As a result, it was confirmed that the rice bran extract increased the skin moisture content (Fig. 1B) and significantly decreased the thickness of the skin layer that was thickened by ultraviolet irradiation (Figs. 2A and 2B). In addition, the rice bran extract has the effect of improving the wrinkles of the skin of the back of the experimental animals and significantly reducing the wrinkle area, depth and length of the skin to significantly suppress the generation of wrinkles (Figs. 3A and 3B) -13 gene expression was significantly decreased, thereby increasing the synthesis of collagen protein and inhibiting collagen fiber degradation, thereby effectively suppressing the formation of wrinkles by irradiation of ultraviolet rays (FIGS. 4A and 4B).

Therefore, the composition containing the rice bran extract of the present invention can be usefully used as a material for health functional foods and oral cosmetics that provide skin moisturization, skin wrinkle improvement, skin photoaging improvement effect.

Accordingly, the present invention relates to a health functional food comprising a food composition for skin improvement.

The term " health functional food " in the present invention refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and rings using raw materials and components having useful functions in the human body. Here, 'functional' refers to the structure and function of the human body to obtain nutritional effects and obtain useful effects for health use such as physiological action. The health functional food of the present invention can be prepared by a method commonly used in the art and can be prepared by adding raw materials and ingredients that are conventionally added in the art. In addition, the formulations of the above health functional foods may also be manufactured without limitations as long as they are acceptable as health functional foods. The health functional food composition of the present invention is advantageous in that it does not have side effects that can occur when a medicine is taken for a long time by using food as a raw material, unlike a general medicine, and is excellent in portability and improves skin moisturization, skin elasticity, And / or as an adjuvant for enhancing the skin photoaging effect.

The skin-improving food composition of the present invention may be prepared in any one form selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings.

According to a preferred embodiment of the present invention, the food composition for skin improvement of the present invention comprises a rice bran extract as an active ingredient and is formulated into powders, liquids, tablets, soft capsules, granules, tea bags, instant tea, . The content of the rice bran extract as an active ingredient can be appropriately determined depending on the intended use (for prevention or improvement). Generally, the amount of the rice bran extract contained in the health functional food composition may be 0.1 to 90% by weight of the total food weight. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range. In addition to the rice bran extract, the health functional food composition according to the present invention may contain other components that can give a synergistic effect to the main effect, for example, vitamin C Such as wrinkle-improving compound or green tea extract, Horseshoe chinensis extract, licorice extract, mulberry bark extract, Rhodiola extract, golden extract, and wild ginseng extract.

The health functional food composition formulated in the above-described form can be added directly to the food or can be used together with other food or food ingredients, and can be suitably used according to a conventional method. Examples of food products include dairy products including drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, soups, , Dairy products and dairy products, and all health functional foods in the ordinary sense are included.

When the health functional food composition of the present invention is a drink, it contains the rice bran extract as an essential ingredient at the indicated ratio, and there are no particular restrictions on other ingredients used for the purpose of manufacturing other drink products, and various flavors Or natural carbohydrate as an additional ingredient. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors and synthetic flavors and the like can be used as the flavors other than those described above. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition, the health functional food composition of the present invention can be used as a nutritional supplement, a vitamin, a mineral (electrolyte), a flavor such as a synthetic flavor and a natural flavor, a coloring agent and a thickening agent (cheese, chocolate etc.), a pectic acid and its salt, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the inventive food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The ratio of such additives is not so important, but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the rice bran extract of the present invention.

According to a preferred embodiment of the present invention, the health functional food of the present invention has an advantage of improving skin moisturizing effect by ingesting it as an inner beauty form. The inner beauty refers to an inner beauty food which is called an eating cosmetic or beauty food. The inner beauty food refers to a food which absorbs a variety of ingredients suitable for the skin to change the skin constitution healthily. In consideration of skin condition and lifestyle, you can choose inner beauty food suitable for each person. More preferably, when the cosmetic composition containing the cosmetic composition and the inner beauty food containing the compound are mixed, the skin whitening improvement effect is significantly higher than that of the cosmetic composition alone, and thus the skin moisturizing effect can be more effectively improved I have.

The present invention also relates to a quasi-drug or a pharmaceutical composition having an effect of improving skin moisturization, improving skin elasticity, improving skin wrinkles and / or improving skin photoaging, which contains rice bran extract as an active ingredient.

When the rice bran extract of the present invention is used as a medicine, it may further contain one or more active ingredients showing the same or similar functions. For example, a known skin wrinkle-improving component or elasticity-enhancing component may be included. The addition of the additional skin wrinkle improving ingredient and the elasticity enhancing ingredient can further improve the wrinkle improving and elasticity improving effect of the composition of the present invention. When the above ingredients are added, skin safety, easiness of formulation, and stability of effective ingredients can be considered according to the combined use. In one embodiment of the invention, the pharmaceutical composition is a wrinkle improving ingredient known in the art and is selected from the group consisting of vitamin C, retinoic acid, TGF, animal placenta-derived proteins, betulinic acid and chlorella extract The above-mentioned skin wrinkle improving component may further be included. The additional skin wrinkle improving ingredient may be contained in an amount of 0.0001 to 10% by weight based on the total weight of the composition. The content range may be adjusted according to requirements such as collagen synthesis promoting activity, skin safety, and ease of formulation of rice bran extract It will be possible.

In addition, the pharmaceutical composition for skin moisturizing of the present invention may further comprise a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers may contain various components such as buffer, injectable sterile water, normal saline or phosphate buffered saline, sucrose, histidine, salts and polysorbates, and the like.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and can be administered in the form of a general pharmaceutical preparation, for example, various forms of oral and parenteral administration at the time of clinical administration. In the case of formulation, , An extender, a binder, a wetting agent, a disintegrant, a surfactant, and the like. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may be prepared by mixing the pharmaceutical composition of the present invention with at least one excipient such as starch, calcium carbonate, Sucrose or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of suppository bases include witepsol, marcogolol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention can provide desirable skin moisturizing, skin elasticity enhancement, skin wrinkle improvement or skin photoaging improvement effects when the effective amount of rice bran extract is included. In the present invention, the term "effective amount" refers to the amount of a compound capable of inhibiting or inhibiting the generation of wrinkles of the skin or alleviating the wrinkles already formed. The effective amount of the rice bran extract contained in the composition of the present invention will vary depending on the form in which the composition is commercialized, the manner in which the compound is applied to the skin, and the time on the skin. For example, when the composition is commercialized as a medicament for dermatological treatment due to wrinkles of skin, reduction of elasticity, and stain, the composition may contain a rice bran extract at a higher concentration than a commercial product that is applied to skin on a daily basis There will be.

The present invention also relates to a method for producing a rice bran extract which is effective in protecting the active ingredient of rice bran by inhibiting rancidity to apply the rice bran extract to health functional foods and oral cosmetics and improving the flowability (fluidity) To a method for preparing a skin composition for improving skin.

According to a preferred embodiment of the present invention, the production method of the present invention comprises: extracting rice bran with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof; Concentrating the rice bran extract obtained in the above step; Mixing the rice bran extract concentrate obtained in the above step with polyoxyethylene sorbitol monolaurate, sodium caseinate and tocopherol; And spray drying the mixture obtained in the step.

According to a preferred embodiment of the present invention, in the manufacturing method of the present invention, the rice bran used in the step of extracting the rice bran can be extracted by using a raw material within 3 days after the bran . Table 1 below shows the change in the acid value of the rice bran material according to the storage days.

Item Control Days saved 1 day 2 days 3 days 5 days 7 days 15th Room temperature 13.5 18.1 20.9 23.2 33.8 45.8 71.3 cold storage 13.5 16.4 16.6 19.6 21.2 24.1 27.5 35 ℃ 13.5 20.2 23.5 26.4 42.5 58.2 90.5

According to a preferred embodiment of the present invention, in the manufacturing method of the present invention, in order to improve the flowability of rice bran powder and prevent rancidity, the mixing step may include adding polyoxyethylene sorbitol monolaurate, casein sodium And tocopherol (natural antioxidant) are added and mixed.

According to a preferred embodiment of the present invention, in the manufacturing method of the present invention, the mixing step is performed by adding 0.5 to 1.5 parts by weight of polyoxyethylene sorbitol monolaurate, 2 to 5 parts by weight of casein sodium and 0.005 to 0.1 part by weight of tocopherol Mixing is preferred. If the content is out of the above range, the flowability of the powder may be drastically lowered.

The skin-improving food composition comprising the rice bran extract powder produced by the method of the present invention prevents rancidity, maintains the content of active ingredient for a long period of time and is excellent in flowability of powder, And can be utilized in various ways.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these examples are for illustrative purposes only and that the scope of the present invention is not construed as being limited by these examples.

Example 1. Preparation of rice bran extract

When the rice bran layer is removed from the rice during the ripening process, the acidosis of the rice bran becomes rapid due to the action of lipase. After 5 days, the acidity increased rapidly. Rice bran extracts were prepared from rice bran at the temperature of 40 ± 3 ℃ for 8 hours at 30 ~ 50 ℃ and for 2 ~ 16 hours. The extract of rice bran was confirmed to have excellent skin moisturizing effect due to its high content of active ingredient, and rice bran ethanol extract was prepared under the above conditions. Table 2 shows the yield and the content of the active ingredient of the rice bran ethanol extract according to the extraction conditions.

Extraction time (hr.) 2 4 6 8 10 16 30 ℃ Yield (%) 12.6 16.6 19.2 20.1 20.1 20.3 Indicator substance (mg / g) 2.9 3.2 3.4 3.7 3.7 3.5 40 ℃ Yield (%) 17.7 22.1 24.4 25.1 25.1 26.3 Indicator substance (mg / g) 3.6 4.0 4.5 4.6 4.6 4.5 50 ℃ Yield (%) 18.8 23.1 25.5 26.7 26.7 27.5 Indicator substance (mg / g) 3.7 4.2 4.6 4.7 4.7 4.5

Example 2 Preparation of Rice Bran Extract Powder

In order to improve the utility as an inner beauty product, it is required to manufacture it as a ring, capsule, or tablet formulation. Thus, a rice bran extract powder having flowability suitable for preparing the above formulation was prepared. The rice bran extract prepared in Example 1 was filtered and concentrated using a vacuum decompression condenser at a temperature of 65 ° C or lower to 13 ± 2 Brix. The concentrated rice bran extract concentrate was added to each concentrate as shown in Table 1 below, homogenized by stirring at 3000 rpm for 10 minutes, sterilized at a temperature of 80 ± 3 ° C for 15 minutes, and sprayed at an inlet temperature of 160 , An outlet temperature of 90 ° C and a flow rate of 8-10 L / h to prepare a rice bran extract powder.

Tween 20 Casein sodium Tocopherol Example 2 1.2 3 0.01 Comparative Example 1 - - - Comparative Example 2 0.3 - - Comparative Example 3 0.8 - - Comparative Example 4 1.2 - 0.01 Comparative Example 5 1.7 - 0.01 Comparative Example 6 1.2 One 0.01 Comparative Example 7 - 3 0.01 Comparative Example 8 1.2 6 -

Experimental Example 1. Determination of Flowability of Rice Bran Extract Powder

To determine the flowability of the powder to the rice bran extract powder of Example 2 and Comparative Examples 1 to 8, 30 g of each sample was collected and stirred at 100 rpm for 2 minutes, and the degree of coagulation and fluidity were measured. The degree of coagulation was visually confirmed, and the evaluation was made on the basis of no coagulation phenomenon of the powder particles (X), a slight coagulation phenomenon (?), A coagulation phenomenon (O), and a large amount of coagulation phenomenon (?). In addition, the flow rate (cm3 / sec) is measured between the upper and lower ends of a glass tube having an inner diameter of 3.5 cm and a length of 60 cm, And the fluidity of the powder was evaluated. The results are shown in Table 4 below.

Cohesiveness liquidity Example 2 × 115 Comparative Example 1 ◎ 52 Comparative Example 2 ◎ 58 Comparative Example 3 ◎ 50 Comparative Example 4 ◎ 49 Comparative Example 5 ◎ 53 Comparative Example 6 ○ 71 Comparative Example 7 ○ 81 Comparative Example 8 X 105

EXPERIMENTAL EXAMPLE 2 Change of Roughness of Rice Bran Extract Powder

To determine the acid value of the rice bran extract powder of Example 2 and Comparative Examples 1 to 8, 100 g of rice bran was weighed to measure the acid value of the rice bran, and 300 ml of an extraction solvent (n-hexane) After the extraction, the filtrate was filtered through anhydrous sulfuric acid, and the filtrate was concentrated under reduced pressure to obtain a retentate. About 1 ~ 3g of the test sample is taken into an Erlenmeyer flask and dissolved in 10 ml of a mixture of ethanol and ether (1: 2). Phenolphthalein is used as an indicator and 0.1N alcoholic KOH solution is added until the pale red color persists for 30 seconds. The results are shown in Table 5 below.

Item storage duration Control 1 day 5 days 15th 30 days 60 days Example 2 16 16.2 16.8 17.1 17.9 18.5 Comparative Example 1 16 19.2 21.5 30.4 42.5 75.4 Comparative Example 2 16 18.0 19.2 26.8 39.2 62.5 Comparative Example 3 16 18.2 20.2 28.7 31.2 63.4 Comparative Example 4 16 17.5 19.2 30.2 39.8 55.4 Comparative Example 5 16 16.9 20.2 28.2 40.2 51.5 Comparative Example 6 16 17.8 20.2 22.2 28.5 32.5 Comparative Example 7 16 18.4 19.8 24.5 36.8 47.5 Comparative Example 8 16 18.1 22.2 30.6 41.2 54.6

Example 3: Test materials, breeding of experimental animals and ultraviolet irradiation

The extract of rice bran was prepared by the methods of Examples 1 and 2, and a dandelion extract having a "physiological activity level 2" as a positive control and "skin moisturizing" function was selected from the Food and Drug Administration. The dandelion extract was prepared by adding 80% ethanol to 18 g of dandelion powder (100% domestic, clear seeds, Kangwon Do, Hongcheon) and 24 hours of the extraction. The extract was concentrated using a rotary evaporator (SB-1200, EYELA, Tokyo, Japan) and powdered by freeze-drying.

The 6-week-old female hairless mouse (SKH-1 hairless mouse) used in this example was purchased from Central Korea (Seoul, Korea) and used after being adapted for 1 week. The experimental animals were divided into 5 groups and 10 animals were assigned to each group. The experimental group was divided into the normal control group (normal), the UV group, the rice bran extract (0.1% diet or 0.5% diet) or the dandelion extract group. During the breeding period, feed and water were freely consumed. The temperature was maintained at 23 ± 2 ° C, the humidity was maintained at 40 ~ 60%, and the light period and the dark cycle were adjusted to 12 hours each day. In the rice bran extract group, 0.1% or 0.5% of rice bran extract prepared by the method of the present invention was added for 16 weeks, and the dandelion extract group was supplemented with 0.5% of the dandelion extract prepared by the above method. Day. The diets were supplied with water daily at 2 pm, and the dietary intake was measured daily. During the experimental period, ultraviolet B (UVB) was irradiated three times a week on the dorsal part of the hairless mouse. The ultraviolet irradiation dose was 1 MED (1 MED = 0.5 J / ㎠, MED; minimal erythema dose) 2 MED, 3 MED at 3 weeks, and 4 MED at 4 to 16 weeks. Weights were measured weekly during the breeding, and photographs of the back skin were taken. On the last day of the experiment, the animals were sacrificed for 6 hours and anesthetized, blood and skin tissue were collected.

Experimental Example 3. Changes in moisture content of skin tissue of ultraviolet irradiated mouse (Figure 1, a, b)

The moisture content of skin such as hairless mice was evaluated by skin-capacitance measurement (Skin-O-Mat, Cosmomed, Germany) to check skin moisturizing effect of rice bran extract. 5%) humidity. For further experiments, the mean of the last five measurements was taken as the moisture value of each animal when the standard deviation for five or six consecutive measurements on the day before euthanasia was less than 0.1.

UV irradiation, rice bran extract, and dandelion extract did not significantly affect body weight and dietary intake of reared mice (Fig. 1a). In the + UVB control group irradiated with UV rays for 16 weeks, the water content of the skin tissue was significantly decreased while the erythema index was significantly increased (Fig. 1B and 1C), compared with the UV irradiation group (-UVB). In the group fed with rice bran extract, the water content was significantly increased by 20% compared to the control group of UVB, even though the same UV intensity was irradiated. Especially, in the group fed with rice bran extract (0.5%), the water content was significantly (Fig. 1B).

EXPERIMENTAL EXAMPLE 4 Change in Skin Thickness of Ultraviolet Irradiated Mouse (FIG. 2, a, b)

As the photoaging by ultraviolet rays proceeds, the formation of the stratum corneum is increased to thicken the skin to protect the skin dermis layer, and thus the thickness of the skin skin layer is increased by ultraviolet irradiation, which means that skin damage due to photoaging is large J Molecular mechanism of skin aging Mech Ageing Dev. 123: 801-810, 2002). In order to confirm skin damage caused by photoaging, dorsal skin tissues of uninjured mice were fixed on 10% neutral formalin on the last day of experiment and then stained with hematoxylin and eosin (H & E), and examined by fluorescence microscope (Nikon Eclipse Ti- S, Tokyo, Japan) and images were analyzed by Metamorph (Molecular Devices, Danville, PA) software.

As a result, the skin thickness was significantly increased in the + UVB control group compared to the non-irradiated normal group (-UVB), and the skin thickness of the rice bran extract group was significantly reduced by 42.6% (Fig. 2A). As a result of H & E staining of skin tissues, the skin surface layer thickness of unhybridized mice was observed to be higher than that of the normal group (-UVB) not exposed to ultraviolet rays in the + UVB control group. In the case of the rice bran extract- It was confirmed that the thickness of the skin layer thickened by ultraviolet irradiation was remarkably reduced (Fig. 2B).

EXPERIMENTAL EXAMPLE 5 Change in Skin Wrinkle Formation of Ultraviolet Irradiated Mouse (FIG. 3, a, b)

To evaluate the effect of rice bran extract on the formation of skin wrinkles, the back skin area of the experimental animals was photographed. The skin of hairless mice exposed to ultraviolet rays for 16 weeks was molded into silicone rubber and the degree of formation of wrinkles was measured. A disk with a hole was attached to the dorsal part of the hairless mouse, and the silicone polymer (Silfo, Flexico Developments LDT, England) reagent was mixed and thinly spread on the back of the hairless mouse, completely dried and then carefully removed to prepare a simulated plate . The wrinkle curves of the skin were evaluated by using a wrinkle measuring device (Visioline VL650, Courage-Khazaka Electronic), and various skin characteristics were measured with a parallel light source and b / w CMOS camera.

The + UVB control group, which was repeatedly exposed to UVB for 16 weeks, was able to observe abnormally increased fine wrinkles with many coarse and deep wrinkles compared with the normal UV group (-UVB) In the rice bran extract group, the thickness and depth of the wrinkles were significantly decreased compared with the + UVB control group. Furthermore, the high concentration of rice bran extract group showed improvement similar to the skin condition observed in the UVB-unvulcanized group (Figs. 3A and 3B). The wrinkle - reducing effect of the rice bran extract was superior to the wrinkle - improving effect observed at the same concentration of the dandelion extract.

EXPERIMENTAL EXAMPLE 6. Confirmation of Inhibitory Effect of Rice Bran Extract on UV-induced COX-2 Expression (FIGS. 4A and 4B)

When irradiated with ultraviolet light, collagenase (collagenase), which is a collagenase, is increased in skin tissue, which mediates skin aging. In addition, the expression of COX-2 induced by ultraviolet irradiation is involved in the inflammatory reaction. To investigate the effect of rice bran extracts on the expression of COX-2 and MMP-13 by ultraviolet light, the skin tissues of reared mice were placed in a 2-mL microcentrifuge tube containing lysis buffer and stainless steel beads and incubated with TissueLyser II (Qiagen, Valencia, CA , USA) at 20 Hz for 2 minutes. The tissue homogenate was centrifuged at 12,000 rpm for 20 minutes. The protein was centrifuged and the supernatant was taken and the protein concentration was measured using a DC Protein Assay kit (Bio-Rad Laboratories). Skin tissue extracts were separated by SDS-PAGE electrophoresis. After transferring to a nylon membrane, the desired protein was separated using an antibody. The skin thickness and MMP-13 and COX-2 expression were visualized using a fluorescence microscope (Nikon Eclipse Ti-S, Tokyo, Japan) and images were analyzed by Metamorph (Molecular Devices, Danville, PA) software.

As a result, as shown in FIG. 4A, the expression of COX-2 and MMP-13 was increased by ultraviolet irradiation in the + UVB control group, and COX-2 and MMP-13 expression thus increased in the rice bran extract- (Fig. 4b), which was also the same in the tissue dyeing.

EXPERIMENTAL EXAMPLE 7 Confirmation of MMP-13 and COX-2 Expression Inhibitory Mechanism (FIGS. 5A and 5B)

MAPK signals are known to play a central role in UV-induced MMP-13 and COX-2 expression. In order to examine whether rice bran extract inhibits ultraviolet-induced MAPK signal cascade in mouse skin tissues to reduce MMP-13 and COX-2 production, JNK1 / 2, ERK1 / 2 and p38 MAPK proteins The effect of rice bran extract on phosphorylation was examined.

Total JNKl / 2, ERK1 / 2 and p38 MAPK protein phosphorylation levels were measured by Western blot analysis as shown in Figures 5a and 5b. In other words, ERK1 / 2, JNK1 / 2, and p38 MAPK protein phosphorylation was induced in the + UVB control group, whereas UVB-induced ERK1 / 2, JNK1 / 2, and p38 MAPK protein phosphorylation was decreased in the rice bran extract group. Thus, rice bran extract significantly inhibited UVB-induced MMP-1 and COX-2 expression through ERK1 / 2, JNK1 / 2 and p38 MAPK pathway inhibition.

Production Example 1. Preparation of Health Functional Foods

A health functional food containing rice bran extract was prepared with the following composition.

1-1. Manufacture of Health Functional Foods

Vitamin B acetate 0.1 mg, vitamin B 0.15 mg, vitamin B2 0.5 mg, vitamin B6 0.2 占 퐂, vitamin B12 10 mg, vitamin C 10 占 퐂, biotin 1.7 mg, nicotinic acid Amide 50 mg, folic acid 0.5 mg calcium pantothenate qs, mineral mixture qs ferrous sulfate 1.75 mg zinc oxide 0.82 mg magnesium carbonate 25.3 mg potassium monophosphate 15 mg potassium diphosphate 55 mg potassium citrate 90 mg, 100 mg of calcium carbonate, 24.8 mg of magnesium chloride

1-2. Manufacture of health drinks

1000 mg of rice bran extract, 1000 mg of citric acid, 100 g of oligosaccharide, 2 g of plum concentrate, 1 g of taurine,

1-3. Manufacture of tablets

1000 mg of rice bran extract, 100 mg of corn starch, 100 mg of lactose, 2 mg of magnesium stearate

1-4. Preparation of capsules

1000 mg of rice bran extract, 100 mg of corn starch, 100 mg of lactose, 2 mg of magnesium stearate

1-5. Manufacture of rings

1 g of rice bran extract, 1.5 g of lactose, 1 g of glycerin, 0.5 g of xylitol

1-6. Manufacture of granules

1000 mg of rice bran extract, 50 mg of soybean extract, 200 mg of glucose, 600 mg of starch

Production Example 2. Preparation of ointment for skin

0.5% by weight of rice bran extract, 8.0% by weight of diethyl sebacate, 5.0% by weight of syrup, 6.0% by weight of polyoxyethylene oleyl ether phosphate,

Preparation Example 3. Preparation of cosmetics

3-1. Production of flexible lotion

0.1% by weight of rice bran extract, 10.0% by weight of ethanol, 1.0% by weight of polyoxyethylene sorbitan polylauric acid, 0.2% by weight of p-oxybenzoic acid methyl, 5.0% by weight of glycerin, 6.0% by weight of 1,3- , Coloring matter amount

3-2. Manufacture of nutrition cream

0.2 weight% of rice bran extract, 15.0 weight% of stearic acid, 1.0 weight% of cetanol, 0.7 weight% of potassium hydroxide, 5.0 weight% of glycerin, 3.0 weight% of propylene glycol, an antiseptic proper amount,

3-3. Manufacture of packs

0.05 wt% of rice bran extract, 5.0 wt% of glycerin, 4.0 wt% of propylene glycol, 15.0 wt% of polyvinyl alcohol, 1.0 wt% of polyoxyethylene oleyl ether, 0.2 wt% of methyl oxy p-oxybenzoate, , Incense volume

Claims (10)

A composition for improving skin comprising an extract of rice bran as an active ingredient. The composition for skin improvement according to claim 1, wherein the rice bran extract is obtained by extracting rice bran with water, an organic solvent or a mixture thereof. The composition for skin improvement according to claim 1, wherein the rice bran extract is prepared by extracting rice bran with a solvent, concentrating it, and spray drying the same. The composition for improving skin according to claim 1, wherein the rice bran extract is a rice bran powder prepared by mixing all or part of polyoxyethylene sorbitol monolaurate, sodium caseinate and tocopherol. The composition for improving skin according to claim 1, wherein the food composition for skin improvement is an oral composition. The food composition according to claim 1, wherein the skin improvement is achieved through skin moisturization, skin wrinkle improvement, skin light damage improvement, or skin erythema reduction. A health-functional food comprising the skin-improving food composition of claim 1. The health functional food according to claim 7, wherein the health functional food is an inner beauty food. Extracting the rice bran with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof;
Concentrating the rice bran extract obtained in the above step;
Mixing the rice bran extract concentrate obtained in the above step with polyoxyethylene sorbitol monolaurate, sodium caseinate and tocopherol; And
And spray-drying the mixture obtained in the above step. 10. The method of claim 9, wherein the mixing is performed by adding 0.5 to 1.5 parts by weight of polyoxyethylene sorbitol monolaurate, 2 to 5 parts by weight of casein sodium, and 0.05 to 0.1 part by weight of tocopherol. ≪ / RTI >

Images