JP2002068993A - Epidermal keratinizing cell propagation-accelerating agent, beautifying food and skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new epidermal keratinizing cell-propagating agent, beautifying food or a skin cosmetic, capable of accelerating metabolism of the epidermal layer of skin and having a high safety. SOLUTION: This epidermal keratinizing cell propagation-accelerating agent is obtained by extracting an embryo in the seeds of Nelumbo nucifera Gaerin belonging to the genus Nelumbo of Nympheae with water or various organic solvents e.g. a lower aliphatic alcohol such as methanol, ethanol, a polar solvent such as acetone, 1,3-butylene glycol, a moderately polar solvent such as chloroform, ethyl acetate, or further a mixture of water with them. Also, by using the epidermal keratinizing cell propagation-accelerating agent, the beautifying food such as a candy, refreshing beverage, tablet and the skin cosmetic such as a cream, lotion, pack can be obtained.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an epidermal keratinocyte proliferation promoter, a cosmetic food, and a skin cosmetic. Specifically, an epidermal keratinocyte proliferation promoter that activates keratinocytes, which is a cell that constitutes most of the human epidermis, and a cosmetic food and skin using the keratinocyte proliferation promoter Related to cosmetics.

[0002]

2. Description of the Related Art The epidermis has a four-layer structure starting from the lowermost layer, the basal layer, and continuing to the spinous layer, the granular layer, and the stratum corneum. Most cells in each layer are composed of the basal layer. Keratinocytes differentiated from Normally, keratinocytes are born in the basal layer, gradually differentiate into the upper layer, migrate and become keratinocytes, constitute the stratum corneum, and finally fall off as dirt from the stratum corneum.

[0003] The stratum corneum is present in the outermost shell of the skin, and constantly turns over to maintain a certain thickness and water content, thereby serving as a barrier against external stimuli. In order to provide this barrier function in the skin, the cycle (keratinization) of keratinocytes from being born in the basal layer to becoming flaked and peeling off (keratinization) is usually repeated at a cycle of four weeks, thereby performing metabolism of the epidermis. However,
The metabolic function of the stratum corneum also deteriorates due to aging, and skin troubles such as wrinkles, dullness, pigmentation, and rough skin occur.

Conventionally, treatment of cuts, cracks such as burns,
Accelerating or healing wound healing
Various keratinizations such as hyaluronic acid, allantoin and its derivatives, bovine blood cell-removing protein, sicon extract, ginseng extract, and placenta extract in skin cosmetics for the purpose of preventing skin aging such as dullness, pigmentation and rough skin, and activating cells A cell growth promoter is included.

[0005] However, depending on the symptoms, an external preparation for skin containing the above-mentioned medicinal ingredients cannot provide a sufficient effect. Therefore, an agent having a more pronounced epidermal keratinocyte proliferation activity has been desired.

[0006] In recent years, the usefulness of so-called functional foods has been pointed out, but nothing that enhances the function of the stratum corneum is known. Skin condition is closely related to health condition, and human health depends largely on diet. Therefore, it is highly desirable that foods enhance the function of the stratum corneum from the inside and lead to healthy skin. Therefore, a functional food that enhances the function of the stratum corneum that is safe for the human body and a stratum corneum function promoting substance that realizes this are desired.

[0007]

The inventors of the present invention have conducted intensive studies and, as a result, have found that some of the lotus seeds that have been eaten or used as a Chinese medicine since ancient times and whose safety has been established have been established. It has been found that the lotus germ extract extracted by using the lotus germ has an epidermal keratinocyte proliferation action, and has completed the present invention.

[0008]

Means for Solving the Problems The epidermal keratinocyte proliferation promoter according to the present invention is characterized by containing a lotus germ extract. The epidermal keratinocyte proliferation promoter referred to in the present invention has an action of promoting the proliferation of epidermal keratinocytes, that is, promoting the division of keratinocytes in the basal layer, as described above. A substance whose action can be confirmed by the law.

In the present invention, an embryo (germ) in a seed of a lotus (Nelumbo nucifera Gaertn.) Belonging to the genus Lotus of the convulsive family is used. Embryos are usually green and rod-shaped, and those that are taken out from collected seeds and dried raw or immediately dried are preferably used.

[0010] The lotus germ extract is prepared by adding the germ to water or various organic solvents, for example, lower aliphatic alcohols such as methanol and ethanol, polar solvents such as acetone and 1,3-butylene glycol, and chloroform and ethyl acetate. It is obtained by extraction using an intermediate polar solvent or a mixture thereof, or a mixed extraction solvent of water and these organic solvents.

In the extraction, the germ obtained from the lotus seed can be used as it is, but it is preferable to use the germ in advance in order to increase the extraction efficiency. In addition, if a non-aqueous solvent (a non-polar solvent) such as hexane is used in advance to perform a degreasing treatment, the content of impurities is reduced, and a more effective extract is obtained.

[0012] The extraction can be carried out at room temperature or under heating by using any apparatus depending on the extraction solvent. For example, crushed lotus germ is put into a treatment tank filled with an extraction solvent, and a soluble component is eluted with occasional stirring. Thereafter, the residue is removed by filtration to obtain an extract. It is preferable that the amount of the solvent used at this time is approximately 1: 5 to 1:20 by weight relative to the lotus germ.

The extract (liquid) thus obtained can be used as it is as an agent for promoting epidermal keratinocyte proliferation. However, the extract obtained has a low epidermal keratinocyte proliferation activity in some cases. As long as the reduction is not hindered, the extract may be provided as a concentrated solution obtained by concentrating the extract, or an extract obtained by further concentrating the extract, or a dried product obtained by evaporating the extraction solvent and the like.

Since the extract of lotus germ has a unique smell and taste, it is preferable to purify the extract for the purpose of deodorizing and decoloring to the extent that its action is not reduced. Of course, depending on the purpose of use, for example, when it is contained in the cosmetic food or skin cosmetic according to the present invention, it is not used in a large amount, so that it can be used without any practical use.

Further, the epidermal keratinocyte proliferation-promoting agent of the present invention may contain various sugars such as sucrose and lactose, proteins and starch in the above-mentioned extract, if necessary, in order to facilitate storage and handling thereof. Excipients and any other auxiliaries may be used. In addition, it can be provided in the form of an appropriate formulation, and can be provided in any dosage form such as, for example, a powder, a granule, and a tablet.

The epidermal keratinocyte proliferation promoter can be used orally by adding it to any food or as a cosmetic food such as a dietary supplement containing these as a main component. Foods that can be added include, for example, various soft drinks and fruit juice beverages, Japanese and Western confectionery, dairy products and other livestock processed products, fruit processed products, vegetable processed products, processed cereal products, processed marine products, seasonings, vitamins Numerous foods and drinks such as so-called various health foods containing as a main component. In addition, it can be provided as a dietary supplement, for example, in the form of a liquid or a tablet. In this case, the addition rate varies depending on the form of the extract such as liquid or solid, taking into account the general intake of the cosmetic food to be added, but generally the intake of the lotus germ extract per adult per day It is appropriate to add about 1 to 1000 mg in terms of the amount, which is about 3 to 6000 mg in terms of dry matter of the lotus germ as a raw material.

In order to exhibit the action of the lotus germ extract most effectively, it is necessary to mix it with various skin cosmetics. Skin cosmetics suitable for the formulation are, for example, ointments, creams, emulsions, lotions, packs, bath preparations and the like. In this case, the blending amount is about 0.01 to 10% by weight as a lotus germ extract, It is appropriate to add so that it becomes approximately 0.04 to 40% in terms of dry lotus germ. In order to maintain the health of the scalp, it is also preferable to mix the lotus germ extract, which is the epidermal keratinocyte proliferation promoter of the present invention, with tonics, rinses, hair growth / hair restoration agents, and the like. Needless to say, the amounts of these components are only guidelines, and even if they are less than or equal to the above range, they can be blended beyond them.

In the cosmetic food and skin cosmetic according to the present invention, various raw materials usually used in the production of cosmetic food and skin cosmetic are used as long as they do not hinder the promotion of skin keratinocyte proliferation. It goes without saying that you can do it.

The present invention can be applied not only to human skin but also to animal skin. In addition, the skin cosmetic of the present invention can be simply applied to healthy skin to prevent skin aging. Not only those that contribute to the treatment of cuts, cracks such as burns, irritations, skin application agents for the purpose of promoting wound healing such as soreness, etc.
It means a skin application agent widely applied to human or animal skin.

[0020]

EXAMPLES Next, skin keratinocyte proliferation promoters, which are examples of the present invention, and cosmetic foods and skin cosmetics, which are formulation examples of the present invention, will be described, and the present invention will be further described in detail.

(Example 1) 300 g of the crushed lotus germ was put into 2000 ml of an extraction solvent shown in Table 1,
After keeping the temperature at 0 ° C and extracting with stirring for 2 hours, the mixture was filtered using a filter paper to obtain an extract. This extract was concentrated at 40 ° C. under reduced pressure, and dried with a vacuum drier to obtain a powdery lotus germ extract, a skin keratinocyte proliferation promoter. The yield is shown in Table 1.

[0022]

[Table 1]

Example 2 300 g of a lotus crushed lotus germ was mixed with 1,3-butylene glycol / water (volume ratio 1: 1).
After pouring into 2000 ml, keeping the temperature at 70 ° C., extracting for 2 hours with stirring, the mixture was filtered using a filter paper to obtain an extract. This extract was allowed to stand at 5 ° C. for 10 days, and the resulting precipitate was subjected to suction filtration using diatomaceous earth to obtain 1,460 ml of a skin lotion extract of skin keratinocytes, which is a liquid lotus embryo extract.
2 ml of the filtrate was placed in a petri dish of known weight, the solvent was evaporated on a steam bath, and further dried at 105 ° C., and the solid content concentration was measured and calculated.
/ V%.

Next, using the various skin keratinocyte proliferation promoters obtained in Example 1, various cosmetic foods and skin cosmetics according to the present invention were prepared. (Formulation Example 1) [Tablet Beauty Food] Lotus Germ Ethanol / Water Mixture (1: 1) Extract 100 (parts by weight) Collagen 30 Mucopolysaccharide / Protein (Chondroitin) 10 Hyaluronic Acid January Pink Peach Extract 33 Black Rice Extract (including polyphenols) 33 Licorice extract (including glycyrrhizin) 10 Milk protein hydrolyzate (including amino acids and low molecular peptides) 10 Vitamin B group mixed powder (including all vitamin B groups) 4 Vitamin C 10 powder Sugar (sucrose) 47 Glycerin fatty acid ester 12 The above component amounts were taken, and a tablet-shaped health and nutritional supplement, which is a cosmetic food according to the present invention, was produced according to a conventional method.

(Formulation Example 2) [Granular cosmetic food] Lotus germ water extract 200 (parts by weight) Pearl protein hydrolyzate (including various amino acids and peptides) 15 Silk protein hydrolyzate 15 Soy isoflavone 10 Purple rice Extract (including polyphenols) 100 Red wine extract powder (including procyanidin) 30 Yeast extract (including glutathione, nucleic acids, amino acids, and vitamins) 10 Pomegranate seed extract 10 Wheat germ extract (including vitamins, chromium, selenium, and molybdenum) 10) DNA 30 Beet oligosaccharide 1060 Stevia extract 10 The above component amounts were taken, and a granular health and nutritional supplement, which is a cosmetic food according to the present invention, was produced according to a conventional method.

(Formulation Example 3) [Capsule-shaped cosmetic food] Lotus germ Ethanol / water mixture (1: 1) extract 50 (parts by weight) Ceramide 30 Phospholipid (lecithin) 10 Vitamin E (tocopherol) 17 Multicarotene ( α and β carotene, lutein, lycopene 10 red rice extract (including cycloartenol ester) 33 octacosanol 1 plant sterol 5 perilla seed oil (including α-linolenic acid) 20 purified fish oil (including DHA, EPA) REFERENCE SIGNS LIST 20 Sesame oil (including lignan compound) 20 Olive oil 10 Soybean and rapeseed mixed oil 62 Glycerin fatty acid ester 12 Take the above component amounts, gelatin capsule according to a conventional method, and obtain capsule-shaped health and beauty food according to the present invention.
Dietary supplements were manufactured.

(Formulation Example 4) [In 100 parts by weight of soft drink aqueous cosmetic food] Lotus germ water extract 1 (parts by weight) Royal jelly 3 Water-soluble collagen 8 Barley extract 1 Ginseng extract (including saponin) 1 Placenta Extract 1 Pueraria extract (including flavonoids) 1 Purple yam extract 1 Oligosaccharide 5 Sucrose 10 Prune juice 2 Pomegranate juice 5 Grapefruit juice 10 Vitamin C 1 Grapefruit flavor 0.7 Water balance Take the above component amounts, stir and dissolve A soft drink-like health / dietary supplement, which is a cosmetic food according to the present invention, was produced.

(Formulation Example 5) [Candy-like cosmetic food] Lotus germ Ethanol / water mixture (1: 1) extract 2 (parts by weight) Mizuna 30 Licorice extract 3 Teng tea extract 1 Blueberry extract (including anthocyan) 1 Green tea Extract (including plant polyphenols) 3 Strawberry 1/5 concentrated juice 1 Red cabbage pigment 0.05 Lemon juice 0.5 Water 5 Take the above component amounts, and according to a conventional method, candy-like health which is a cosmetic food according to the present invention. -Made dietary supplements.

(Formulation Example 6) [Emulsion in 100 ml] Lotus germ ethanol / water mixture (1: 1) extract 1.0 g Stearyl glycyrrhetinate 0.2 g Cetyl alcohol 0.5 g Magnesium ascorbate 3.0 g Beeswax 2.0 g Polyoxyethylene sorbitan oleate (10E.O.) 1.0 g Glyceryl monostearate 1.0 g Hyaluronic acid 0.1 g Propylene glycol 5.0 g Ogon extract 1.0 g Pea seed extract 1.0 g Ethanol 3 0.0g Methyl paraoxybenzoate 0.3g Fragrance 0.03g Remaining amount of purified water The above component amounts were taken, and an emulsion as the skin cosmetic according to the present invention was prepared according to a conventional method.

(Formulation Example 7) [Emulsion 100 ml] Lotus germ ethanol extract 0.1 g Lotus germ 1,3-butylene glycol / water mixture (1: 1) extract 1.0 g Jojoba oil 4.0 g olive oil 2 2.0 g Squalane 2.0 g Cetanol 2.0 g Tocopherol acetate 0.2 g Glyceryl monostearate 2.0 g Polyoxyethylene cetyl ether (20 EO) 2.5 g Polyoxyethylene sorbitan oleate (20 EO) 0 g 1,3-butylene glycol 3.0 g methyl paraoxybenzoate 0.15 g birch bark extract 0.5 g oil-soluble licorice extract 0.05 g cinnabar extract 0.5 g flavoring 0.05 g purified water balance Then, an emulsion as a skin cosmetic according to the present invention was prepared according to a conventional method.

(Preparation Example 8) [In 100 ml of lotion] Lotus germ water extract 0.2 g Lotus germ 1,3-butylene glycol / water mixture (1: 1) extract 2.0 g glycerin 3.0 g 1,3 -Butylene glycol 3.0 g polyoxyethylene sorbitan oleate (20E.O.) 0.5 g methyl paraoxybenzoate 0.15 g citric acid 0.1 g sodium citrate 0.1 g dipotassium glycyrrhizinate 0.3 g lindane extract 0.0 5 g Loquat extract 0.5 g Yeast extract 0.3 g Hyaluronic acid aqueous solution (solid content: 1%) 3.0 g Fragrance 0.05 g Purified water balance A lotion was prepared.

(Preparation Example 9) [In 100 g of cream] Lotus extract of ethanol extract 0.1 g Lotus germ 1,3-butylene glycol-water mixture (1: 1) extract 1.0 g Carrot extract 0.2 g Extract of extract 0.5 g Tormentilla extract 0.5 g Hydrolyzed conchiolin 0.1 g Liquid paraffin 5.0 g Wheat ceramide 0.1 g Salami beeswax 4.0 g Cetanol 3.0 g Squalane 10.0 g Lanolin 2.0 g Stearic acid 1.0 g Oleic acid Polyoxyethylene sorbitan (20E.O.) 1.5 g Glyceryl monostearate 3.0 g 1,3-butylene glycol 6.0 g Methyl parahydroxybenzoate 1.5 g Fragrance 0.1 g Purified water balance A W / O type cream, which is a skin cosmetic according to the present invention, is prepared according to a conventional method. It was.

(Formulation Example 10) [In 100 g of pack] Lotus germ 1,3-butylene glycol / water mixture (1: 1) extract 5.0 g Polyvinyl alcohol 15.0 g Placenta extract 1.0 g Ascorbic acid 0.02 g Pueraria Extract 0.2 g Hypericum extract 1.0 g Polyethylene glycol 3.0 g Propylene glycol 7.0 g Ethanol 10.0 g Methyl parahydroxybenzoate 0.05 g Perfume 0.05 g Purified water residue Was prepared as a skin cosmetic.

(Effect test) [Human epidermal keratinocyte proliferation promoting effect] Obtained in Example 1 above
Based on the following test methods for various lotus germ extracts
The effect of promoting epidermal keratinocyte proliferation was measured by using
Shown in The solvent of each sample solution, 50 v / v% DM
The control value tested using the SO / water mixture was 100
As shown. Test method: Normal human neonatal skin epidermal keratinocytes (NHE)
K) 25cm^TwoHuman epidermal keratinocytes in a flask
37 ° C, 5% CO_TwoCulture in an environment
More keratinocytes were collected. Obtain the keratinocytes in the same medium
2.5 × 10^FourAnd adjust to 200 μl
Seeded into a 48-well plate at a time, 5% CO _TwoUnder environment 3
Cultured overnight at 7 ° C. After culturing, the above lotus germ extract was
0 v / v% DMSO / water
Add 2 μl of sample solution at 37 ° C., 5% CO_TwoUnder the environment
For 3 days. After the culture, the MTT method by Tada et al. (J.I.
mmunol.Methods.93,157,1986)
did.

[0035]

[Table 2]

As can be seen from Table 2, it was confirmed that the agent for promoting the proliferation of keratinocytes according to the present invention has a good promoting action on the proliferation of human keratinocytes.

[0037]

According to the present invention, it is possible to provide a novel highly safe epidermal keratinocyte proliferation promoter. By applying this to cosmetic foods and skin cosmetics, the metabolism of the skin can be promoted, which can greatly contribute to the prevention of skin aging, cell activation, and promotion of wound healing.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/00 A61K 7/00 NMU A61P 17/02 A61P 17/02 17/16 17/16 A23L 2 / 00 FF term (reference) 4B014 GB06 GG17 GK12 4B017 LC03 LG15 LL09 LP01 4B018 LB01 LB08 LE01 LE02 LE05 MD61 ME14 MF01 MF08 4C083 AA032 AA072 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AD122 AC412 AD482 AD532 AD642 AD662 CC02 CC04 CC05 CC07 DD23 DD31 DD41 EE10 EE12 EE13 4C088 AB39 AC04 BA08 NA14 ZA89

Claims (3)

[Claims] 1. An agent for promoting proliferation of epidermal keratinocytes, which comprises a lotus germ extract. 2. A cosmetic food, comprising the epidermal keratinocyte proliferation promoter according to claim 1. 3. A skin cosmetic comprising the epidermal keratinocyte proliferation promoter according to claim 1.