JP2014105167A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic having excellent skin-beautifying and whitening effects and excellent biosafety, by finding novel skin-beautifying and whitening components in natural substances and blending such components, which exhibit excellent prevention and improvement effects against skin aging phenomena such as wrinkles, sagging, dullness, stains, and freckles to keep or improve a skin to a young-looking and sound state and has less skin irritation and excellent biosafety.SOLUTION: A cosmetic comprises components from three or more species of plants of rice, a plant of genus Brassica of the family Brassicaceae, a plant of genus Nelumbo of the family Nymphaeaceae, and adlay. Since the plant components are an extract of the plant, a hydrolysate of the extract, or a fermentation product of the plant, the cosmetic displays excellent whitening and skin-beautifying effects and is excellent in feeling of use by synergistic effect of an excellent skin physiological activity of these plant components.

Description

  The present invention relates to cosmetics having excellent skin beautification and whitening effects and high safety.

  Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging, decreased hormone secretion, quantitative decrease of extracellular matrix components, and cells caused by active oxygen induced by sunlight (ultraviolet rays). -Phenomenon caused by complex intertwining with external factors such as tissue damage or inflammation.

  Reactive oxygen, an external factor of skin aging, not only directly damages skin cells, but also denatures or crosslinks the collagen of the extracellular matrix component, resulting in wrinkle formation and reduced skin elasticity. It causes various damages to the skin, such as causing abnormal pigmentation and causing spots and freckles.

  In order to prevent this skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics containing these skin-beautifying and whitening ingredients have been put on the market. For example, vitamin C, vitamin E, superoxide dismutase (hereinafter abbreviated as SOD), an antioxidant such as catalase; an anti-inflammatory agent such as glycyrrhizic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract Cell activation components such as γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin and hyaluronic acid; and humectants such as urea. Further, arbutin, kojic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots and buckwheat, and are widely used as active ingredients of whitening agents.

However, these conventional skin-beautifying agents and whitening agents have problems in terms of safety to the skin and the like and effectiveness when actually applied to the skin. In addition, various natural ingredients-derived skin-beautifying agents and skin-whitening agents have been proposed (Patent Documents 1 and 2). The skin-beautifying and whitening effects of these agents, when viewed as cosmetic ingredients, are the skin. There are problems in terms of safety against the above and the effectiveness when actually applied to the skin. Accordingly, there is a need for cosmetics containing cosmetic ingredients with improved such points.
JP 62-056410 A Japanese Patent Laid-Open No. 03-188018

  In view of the problems of the prior art, the present inventors have intensively studied to find new skin-beautifying and whitening ingredients derived from natural products from the viewpoint of skin safety. As a result, by using together the ingredients obtained from three or more kinds of plants of rice, Brassicaceae Brassica, water lily family Lotus, and pearl barley, there are excellent skin beautification and whitening effects, and The present inventors have found that it is possible to provide cosmetics with excellent skin safety, and have completed the present invention.

The present invention is a cosmetic comprising three or more plant components of rice, Brassica plant, Nympaeaceae lotus (Nelumbo) plant, and pearl barley, The plant component is an extract of the plant, a hydrolyzate of the extract, or a fermented product of the plant.
In addition, the present invention is a cosmetic characterized by further including any one or more of a whitening agent, a moisturizing agent, a cell activator, an anti-inflammatory agent, an antioxidant and a blood circulation promoter.
In the present invention, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

  The present invention is a cosmetic comprising three or more kinds of plants among rice, Brassicaceae Brassica plant, Lilyaceae lotus plant, and pearl barley, wherein the plant component is an extract of the plant Or, since it is a hydrolyzate of the extract or a fermented product of the plant, it exhibits excellent skin and whitening effects due to the tyrosinase activity inhibitory action, fibroblast activation action, etc. possessed by these plant components. Cosmetics can be provided.

The rice used in the present invention is not particularly limited, and any of brown rice, polished rice, processed rice, pigmented rice (black rice, purple rice, red rice, etc.) can be used. White rice or processed rice is used. As the type of rice, either sticky rice or glutinous rice may be used. In addition, examples of processed rice include antiallergic rice, low protein rice (for example, low glycerin rice), and fortified rice (for example, γ-aminobutyric acid rice). Moreover, the use of white rice and / or red rice contained in brown rice is also possible.

In the present invention, examples of the Brassicaceae Brassica plant include, for example, Brassica alba, Brassica juncea, Brassica nigra, Brassica ropa, and the like. Among these Brassica plants, white cocoon (Brassica alba), yellow cocoon (Brassica juncea) or black cocoon (Brassica nigra) is preferable. The whole plant, seeds, leaves, stems, roots and flowers of these plants can be used, but among them, the use of whole plants or seeds (ie, white coconut, yellow coconut or black coconut) is preferred. .

Examples of the plant belonging to the genus Lotusaceae used in the present invention include lotus (Nelumbo nucifera).
Gaertner) or American kibas (Nelumbo Lutea Pers.) Can be mentioned. Among them, the use of lotus (Nelumbo nucifera Gaertner) is preferable. Any part of these plants such as whole grass, leaves, flowers, stamens, pistils, stems, rhizomes, seeds (grains) and germs may be used, but the use of seeds is preferred.

  The pearl barley used in the present invention is a plant belonging to the genus Giusedama, and is widely used for medicinal purposes and food. In the present invention, any part of pearl barley, leaves, flowers, stems, roots, seeds and the like may be used, but the use of seeds is preferred.

A method for preparing an extract using the above plants will be described below. Preparation of the extract is carried out by conventional methods such as immersion method, countercurrent extraction method, supercritical extraction method, etc., after pre-washing the extraction target site of each plant as necessary, drying, chopping or grinding. By contacting with an extraction solvent.

As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, water or water is used in the present invention from the viewpoint of ease of transition to the treatment step when the extract is subjected to the hydrolysis treatment described below and wide application to cosmetics. The use of a mixed solvent of water and lower alcohols or polyhydric alcohols is preferred. When a mixed solvent is used, the mixing ratio of the lower alcohol or polyhydric alcohol to water is generally 70% by weight or less, preferably 50% by weight or less.

  In preparing the extract, the pH, temperature and time are not particularly limited, but it is preferable to select appropriately depending on the extraction target site of the plant to be used.

For example, in the preparation of rice extract, it is preferable to use an alkaline adjuster such as sodium hydroxide, sodium carbonate, potassium hydroxide, etc., so that the pH is in the range of 7.5 to 14, particularly 9 to 12. The extraction temperature and time in this case vary depending on the type and particle size of the rice, but are generally 4 to 40 ° C. for 6 hours to 7 days, preferably 50 to 60 ° C. for 12 to 36 hours, and 80 ° C. for 1 to 1 hour. 12 hours.

  When preparing the extract of the present invention, when using, for example, white birch, black cocoon, or jaundice seeds belonging to the Brassicaceae Brassica genus as the extraction target site, it is generally preferably in the range of 3-9. In this sense, if necessary, an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid is added to the extraction solvent. You may adjust so that it may become the target pH. Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent to be used, or the shredding degree and particle size of the extract, but for example, in the case of the immersion method using water as the extraction solvent, The extraction temperature is generally in the range of 1 to 90 ° C., preferably 20 to 60 ° C., and the extraction time is, for example, in the case of room temperature in the range of 0.5 to 72 hours, preferably in the range of 1 to 24 hours. .

Moreover, when preparing the extract of the present invention, for example, when using seeds of the genus Lotusaceae as the extraction target site, it is generally preferably in the range of 4 to 8, and in this sense, if necessary Add alkaline adjusters such as sodium hydroxide, sodium carbonate, potassium hydroxide, and acidic adjusters such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, etc. to the extraction solvent so as to achieve the desired pH. You may adjust. Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent to be used, or the shredding degree and particle size of the extract, but for example, in the case of the immersion method using water as the extraction solvent, The extraction temperature is generally in the range of 1 to 90 ° C., preferably 40 to 85 ° C., and the extraction time is, for example, in the range of 0.5 to 72 hours at room temperature, and 1 to 12 hours at 40 to 80 ° C. Range.

Moreover, when preparing the extract of the present invention, for example, when using seeds of the genus Lotusaceae as the extraction target site, it is generally preferably in the range of 4 to 8, and in this sense, if necessary Add alkaline adjusters such as sodium hydroxide, sodium carbonate, potassium hydroxide, and acidic adjusters such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, etc. to the extraction solvent so as to achieve the desired pH. You may adjust. Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent to be used, or the shredding degree and particle size of the extract, but for example, in the case of the immersion method using water as the extraction solvent, The extraction temperature is generally in the range of 1 to 90 ° C, preferably 40 to 85 ° C, and the extraction time is in the range of 0.5 to 72 hours at room temperature and in the range of 1 to 12 hours at 40 to 80 degrees.

In the preparation of the extract of the present invention, for example, when pearl seeds are used as the extraction target site, it is generally preferably in the range of 4 to 8, and in this sense, the extraction solvent described above is used if necessary. In addition, an alkaline adjusting agent such as sodium hydroxide, sodium carbonate, potassium hydroxide or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, etc. may be added to adjust to the desired pH. Good. Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent to be used, or the shredding degree and particle size of the extract, but for example, in the case of the immersion method using water as the extraction solvent, The extraction temperature is generally in the range of 1 to 90 ° C, preferably 40 to 85 ° C, and the extraction time is in the range of 0.5 to 72 hours at room temperature and in the range of 1 to 12 hours at 40 to 80 degrees.

  In the present invention, the extract obtained by the extraction process may be subjected to a hydrolysis process before or after extraction or in parallel with the extraction. Examples of the hydrolysis treatment include a method using an acid, an alkali, an enzyme, and the like, but a hydrolysis treatment using an enzyme is preferable. In the case where hydrolysis is performed using an enzyme, and when a solvent other than water or a mixed solvent of water and a hydrophilic solvent is used for preparing the extract solution, the extract solvent is once removed from the extract solution, The extract obtained here is redissolved in water or a mixed solvent of water and a hydrophilic solvent and then subjected to an enzymatic decomposition treatment.

Examples of enzymes used for the hydrolysis treatment include proteolytic enzymes, starch degrading enzymes, fibrinolytic enzymes, and lipolytic enzymes. These enzymes may be used alone or in combination with a plurality of enzymes depending on the plant species.

Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase, Bromelain, microorganism-derived complex proteolytic enzyme [for example, neurase (manufactured by Amano Enzyme Co., Ltd.)] and the like. When using a proteolytic enzyme, any of the above-described enzymes may be used alone or in combination. Further, as the amylolytic enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase and the like can be used. When using a amylolytic enzyme, any of the above-described enzymes may be used alone, or a plurality of them may be combined. Examples of the fibrinolytic enzyme include cellulase, hemicellulase, and pectinase. When a fibrinolytic enzyme is used, any of the above-described enzymes may be used alone, or a plurality may be combined. Examples of the lipolytic enzyme include lipase. In addition, when performing the hydrolysis process with an enzyme, you may use combining 1 or more types of 1 or more types of enzymes chosen from said each enzyme group.

In the hydrolysis treatment using an enzyme, one or more of the above enzymes are added to the above-described plant extract solution, and the enzyme is reacted under conditions near the optimum pH and temperature of the enzyme used. To be implemented. When two or more kinds of enzymes are used in combination, two or more kinds of enzymes may be allowed to act simultaneously according to the characteristics of the enzyme used, or may be allowed to act sequentially without changing or changing the reaction conditions. . The amount of the enzyme used is preferably in the range of 0.001 to 50 parts by weight, more preferably 0.1 to 10 parts by weight per one enzyme with respect to 100 parts by weight of the solid content of the plant extract solution. Range. The enzyme treatment time varies depending on the type of enzyme used and the like, but is generally in the range of 0.5 to 24 hours, preferably in the range of 1 to 6 hours. In addition, when hydrolyzing the said plant, it is preferable to combine amylolytic enzyme and / or a fibrinolytic enzyme with 1 type, 2 or more types of proteolytic enzymes, or 1 type or 2 types or more of proteolytic enzymes. . Furthermore, it is preferable to use papains, bromelain and / or actinases as proteolytic enzymes.

  After completion of the hydrolysis treatment using the above enzyme, the enzyme is deactivated by using an appropriate method such as a method of heating the enzyme treatment liquid to, for example, 80 ° C. or higher to obtain an enzyme-treated decomposition product solution.

In general, the extract or the hydrolyzed product prepared as described above may be used as a cosmetic compounding agent as it is after adjusting the pH to 4 to 8, or by vacuum concentration or the like. It may be used as a desired concentration. In addition, the extract may be dried by a conventional method such as spray drying.

  In the present invention, a fermented product of the above plant may be used. Examples of microorganisms used for fermentation include lactic acid bacteria, koji molds, natto bacteria, tempeh bacteria, yeasts, etc., and generally one or more selected from any of these bacterial species are used. As long as they do not interfere with each other, they may be used in combination with those belonging to different bacterial species.

Examples of lactic acid bacteria include Lactobacillus plantarum, L. brevis, L. casei and other Lactobacillus lactic acid bacteria; Carnobacterium divergence (Carnobacterium
divergens), lactic acid bacteria of the genus Carnobacterium such as Carnobacterium piscicola; Leuconostoc (Leuconostoc)
mesenteroides), lactic acid bacteria of the genus Leuconostoc such as Leuconostoc citreum; Streptococcus faecalis (Streptococcus)
faecalis), Streptococcus pyogenes and other Streptococcus lactic acid bacteria; Enterococcus caseliflavus, Enterococcus sulfreus and other Enterococcus sulfreus enterococcus (
Enterococcus) lactic acid bacteria; Lactococcus plantarum (Lactococcus)
lactic acid bacteria of the genus Lactococcus such as Lactococcus rafinolactis; Weissella confusa, lactic acid bacteria of the genus Weissella kandleri; Atopobium mintopum ) Lactic acid bacteria of the genus Atopobium; Vagococcus flubiaris (Vagococcus)
lactic acid bacteria of the genus Vagococcus such as fluvialis), Vagococcus salmoninarum; Pediococcus damnosus
damnosus), Pediococcus pentosaceus, and other lactic acid bacteria belonging to the genus Pediococcus. Among these lactic acid bacteria, the use of Lactobacillus plantarum is most preferable from the viewpoint of the skin physiological activity of the obtained fermented product and the ease of handling without being extremely anaerobic.

As the koji mold, for example, Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus soya (Aspergillus soy)
sojae), Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami,
Examples include black koji molds such as Aspergillus niger, Monascus anka, and Monascus pilosus. Among them, Aspergillus oryzae is most preferable from the viewpoint of the skin physiological activity of the obtained fermented product and the relatively low coloring and fermentation odor of the fermentation broth.

Examples of Bacillus natto include Bacillus natto, Bacillus subtilis, Bacillus circulans (Bacillus
bacterium of the genus Bacillus such as circulans). Among these, Bacillus natto is most preferable because it is widely used in foods and has high safety.

Tempe fungi include Rhizopus azygosporus, Rhizopus microsporus chinensis, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus Examples include fungi of the fungus. Among them, Rhizopus microsporus oligosporus and Rhizopus oryzae are most preferable because they are widely used for fermented foods in Southeast Asia including Indonesia.

Examples of the yeast include Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergences, Saccharomyces carlsbergences,
bayon us), Saccharomyces yeast, Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei, Torulaspora rosei, etc. Soya (Zygosaccharomyces
soy), Zygosaccharomyces sake, Zygosaccharomyces miso, Zygosaccharomyces lactis, etc.
etchellsii), Candida kefyr, Candida sake, Candida scottii and other yeasts of the genus Candida, Aureobasidium Pullulans, Aureobasidium mansonii ), Yeast of the genus Aureobasidium such as Aureobasideium microstictum. Among them, Saccharomyces cerevisiae is most preferable because it is most widely used for foods and has a strong fermenting power.

  Preferable specific examples of the method for fermenting a plant using the above microorganisms are as follows. First, a suspension for fermentation is prepared by immersing or suspending a plant to be fermented (hereinafter sometimes referred to as a fermentation material) in a solvent. In this case, the plant may be used as it is, or may be used after previously dried or semi-dried. In addition, as for the shape, the collected one can be used as it is, but the fermentation efficiency can be increased if it is shredded or pulverized and refined.

As a solvent for suspending the fermentation material, water or a mixture of water and lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) In these solvents, sugars such as glucose, fructose and sucrose may be added. However, in view of the fact that microorganisms are most likely to exert their action and there are assimilation components other than plant components. It is particularly preferred to use water alone, in order to avoid the production of fermented by-products based on it.

  Before the fermentation material / solvent suspension is subjected to a fermentation process, it is necessary to sterilize and remove the germs that hinder the fermentation. May be used by washing with ethanol for sterilization in advance and then suspending in a sterile solvent such as sterile water. May be. As the heat sterilization treatment, the autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, or holding at 80 to 90 ° C. for 60 to 120 minutes is repeated once a day for 2 to 3 days. The heat sterilization method such as the method is generally used.

Next, the sterilized suspension is placed in a fermentation tank, and microorganisms are inoculated into the fermentation tank for fermentation. The appropriate amount of inoculated microorganism is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably in the range of 30 to 40 ° C. (for example, 35 ° C. to 40 ° C. for lactic acid bacteria), which is the optimum temperature for growth of each microorganism. The number of days of fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation days are shorter than the above general range, the fermentation is not sufficiently performed and the effectiveness of the fermented product tends to be reduced. On the other hand, if the fermentation days are longer than 10 days, no further increase in effectiveness is observed. Not only that, but also coloring and an increase in fermentation odor occur.

  In performing the above fermentation treatment, an enzyme is added to the suspension before or at the time of inoculation of microorganisms so that plant components can be used more effectively by microorganisms. Hydrolysis treatment with an enzyme may be performed. In this case, examples of the enzyme to be used include the above-mentioned proteolytic enzymes, amylolytic enzymes, fibrinolytic enzymes, and lipolytic enzymes.

  The fermented product obtained as described above is subjected to a solid-liquid separation operation such as filtration to be separated into a liquid phase portion and a solid phase portion. Any of the separated liquid phase portion and solid phase portion may be used. For example, regarding the fermented rice, the solid phase portion may be dried and powdered.

  The cosmetic of the present invention is formed by combining three or more kinds of plants, but the mixing ratio of these components is 10 parts by weight of the rice extract, hydrolyzate or fermented product. The extract, hydrolyzate or fermented product of Brassica plant is generally 0.1 to 100 parts by weight, preferably 1 to 50 parts by weight, and the extract, hydrolyzate or fermented product of Lotus species is generally used. 0.1 to 50 parts by weight, preferably 1 to 30 parts by weight, and pearl barley extract, hydrolyzate or fermented product is generally in the range of 0.1 to 100 parts by weight, preferably 1 to 30 parts by weight. . The amount of each plant component in the cosmetic varies depending on the use and application site of the cosmetic, but is 0.0001% to 10.0% by weight, preferably 0.001 to 5% in terms of solid content. 0.0% by weight.

  Furthermore, in the present invention, it further includes any one or more of a whitening agent, a moisturizing agent, an epidermal cell activator, a fibroblast activator, an antioxidant, an anti-inflammatory agent, and a blood circulation promoter. It is preferable.

  First, whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), AMP (Adenosine monophosphate, adenosine monophosphate) may be mentioned, and these may be blended singly or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like as tranexamic acid derivatives, tranexamic acid esters (for example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, trane And amides of tranexamic acid (for example, tranexamic acid methylamide). Resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, and the like. Examples of the -dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid amide. Benzyl nicotinate and the like, vitamin E derivatives such as vitamin E nicotinate and vitamin E linoleate, and α-hydroxy acids such as lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid is there.

In addition, as a whitening agent derived from natural products such as plants, seaweeds and animals, extraction of cypress, murasakixibu, mulberry, lacquered carrot, chamomile, peonies, buttons, royal jelly, rice bran, sake lees, green algae, brown algae, red algae, amamo, etc. Product, hydrolyzate or fermented product, placenta extract and the like. The use part of the plant may be whole grass, flowers, buds, persimmons, seeds, fruits, pericarps, leaves, stems, roots, root barks and the like. For example, the root carrot is a root (participant), a mulberry is a root bark, a peony is a flower or root, and a button is a flower or root bark (button pi). It is not limited.

In addition, as epidermal cell activators, eggplant (water eggplant, Kamo eggplant, long eggplant, rice eggplant, etc.), hibiscus, rice, salamander, coral grass, green algae, brown algae, red algae, jellyfish and other extracts, hydrolysis Product or fermented product. The use part of the plant may be whole grass, flowers, buds, persimmons, seeds, fruits, pericarps, leaves, stems, roots, root barks, etc., for example, fruits for eggplants, flowers for hibiscus, In the case of rice and rice, examples include leaves, and in the case of salamander, seeds and the like. However, the present invention is not limited thereto.

In addition, as a fibroblast activator, funori, amamo, eggplant (water eggplant, Kamo eggplant, long eggplant, rice eggplant, etc.), mangosteen, mango, cherimoya, royal jelly, liquor and other extracts, hydrolysates or fermented products Is mentioned. The use part of the plant may be whole grass, flowers, persimmons, seeds, fruits, pericarps, leaves, stems, roots, root barks, etc., for example, eggplant, mangosteen, mango, cherimoya fruit etc. However, the present invention is not limited to this.

Examples of the moisturizing agent include extracts such as loofah, aloe, amamo, green algae, brown algae, red algae and jellyfish, hydrolysates or fermented products. The use part of the plant may be whole grass, flowers, buds, persimmons, seeds, fruits, pericarps, leaves, stems, roots, root barks, etc. The invention is not limited to this.

Examples of the anti-inflammatory agent include extracts such as gentian, paudalco, forget-me-not, chamomile, juazeiro, hydrolyzate, or fermented product. The use part of the above plant may be whole grass, flowers, persimmons, seeds, persimmons, fruits, pericarps, leaves, stems, roots, root barks, etc., for example, roots for gentian, bark for powdarco, In the case of forget-me-not, flowers, buds, in the case of chamomiles, in the case of flowers, and in the case of juazeiro, the bark, etc. may be mentioned, but the present invention is not limited thereto.

Antioxidants include extracts such as carrots, rice, peonies, buttons, mulberries, lilies, murasakikibu, soy milk, hydrolysates or fermented products. The plant may be used in whole plants, flowers, persimmons, seeds, persimmons, fruits, pericarps, leaves, stems, roots, root barks, etc., such as carrots (western carrots, ginseng (spotted carrots, ginsengs, etc.) ), Bamboo ginseng, rice ginseng, etc.) if root, leaf if rice, blossom if peonies, root, flowers if roots, buttons, root bark, flowers if lily, In the case of persimmon and murasakikib, examples include fruits, but the present invention is not limited thereto.

In addition, examples of the blood circulation promoter include Tamazaki Rafuji. The use of roots is preferable in the case of Tamatsuki tsumugi, but the present invention is not limited to this.

In addition, the present invention further includes components usually used in cosmetics such as oil components, surfactants (synthetic and natural products), moisturizers, thickeners, antiseptics and sterilizers. An agent, a powder component, an ultraviolet absorber, an antioxidant, a chelating agent, a dye, a fragrance and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the composition of the present invention are not impaired, it may be combined with other physiologically active ingredients in cosmetics.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, legumes, cereals, etc.), juzairo (Rhamnaceae zizyphus joazeiro) An extract or the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seafood-derived collagen and derivatives thereof, various amino acids And their derivatives.

  Examples of the thickener include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; gums such as xanthan gum, tragacanth gum and guar gum; Cellulose derivatives such as methyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and derivatives thereof; polyglutamic acid and derivatives thereof.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof.

  Examples of chelating agents include trisodium ethylenediaminehydroxyethyl triacetate, edetic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyethane diphosphonate, and the like.

  As the physiologically active ingredient, for example, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid), 2,5-dihydroxybenzoic acid derivatives are also used as skin aging preventing / beautifying ingredients, derived from animals or fish. Collagen and derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate And γ-amino-β-hydroxybutyric acid.

The application site of cosmetics (including quasi-drugs) containing the composition of the present invention includes general skin including the scalp, and is not particularly limited. Therefore, the dosage forms include emulsions, creams, lotions, essences, ointments, packs, haps, skin cleansers (soaps, etc.), facial cleansers, shampoos, rinses, treatments, various makeup cosmetics, bath preparations, etc. It can be diverse.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Rice extract 1250 g of 0.025M sodium hydroxide solution was added to 1000 g of polished rice and stirred at room temperature for 21 hours. Solid matter was removed by filtration, and the extract was adjusted to pH 7.5 to obtain 750 g of rice extract (solid content concentration 2.1%).

Production Example 2 Hydrolyzate of rice extract 1250 g of 0.025M sodium hydroxide solution was added to 1000 g of polished white rice and stirred at room temperature for 21 hours. Solids were removed by filtration and the extract was adjusted to pH 7.5. Papain 0.02% and actinase 0.02% were added to this extract, and hydrolysis was performed at 40 ° C. for 2 hours. After the enzyme was inactivated by heating, this solution was filtered to obtain 600 g of a rice extract hydrolyzate (solid content concentration 1.8%).

Production Example 3 100 g of fermented rice polished rice was washed with water, 900 g of purified water was added to prepare a suspension, and heat sterilized. After adding 1.0 g of glucoamylase and 1.0 g of papain to this solution, 10 ⁸ cells / mL of lactic acid bacteria (Lactobacils plantarum) were inoculated, and statically cultured at 37 ° C. for 3 days. After completion of the culture, the culture solution was sterilized by heating and cooled to room temperature to obtain a fermented rice product (solid content concentration 2.5% by weight).

Production Example 4 White coconut extract 100 g of white coconut seed pulverized product and 1000 g of purified water were mixed to adjust the pH to about 5 and extracted at room temperature for 24 hours. : About 1.1% by weight).

Production Example 5 Enzyme-decomposed product of white coconut 50 g of crushed white birch seeds (white coconut) was mixed with 1000 g of purified water, extracted at 40 ° C. for 1 hour, filtered and 805 g of a pale yellow transparent white coconut extract solution ( Solid concentration: 1.2% by weight) was obtained. Next, 0.05 g of actinase AS (manufactured by Kaken Pharma Co., Ltd.) was added to 500 g of the extract solution obtained here, and hydrolyzed at 40 ° C. for 2 hours. Thereafter, the enzyme was inactivated by heating at 85 ° C. for 1 hour, followed by filtration to obtain 460 g (solid content concentration: 1.0% by weight) of a hydrolyzate solution of a pale yellow transparent white coconut extract.

Production Example 6 Fermented white cocoon 100 g of white birch seed (white coconut) was pulverized, and 900 g of purified water was added thereto to prepare a suspension. After adding 0.1 g of proteolytic enzyme (actinase AS; manufactured by Kaken Pharma Co., Ltd.) to this suspension and hydrolyzing at 40 ° C. for 2 hours, extraction and enzyme deactivation treatment were performed at 80 ° C. for 1 hour. After cooling, filtration was performed to obtain a white coconut extract solution. Next, this liquid was sterilized by heating, inoculated with 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum), and cultured at 37 ° C. for 3 days. After completion of the culture, the culture broth was sterilized by heating, cooled to room temperature and filtered to obtain 658 g of a white lactic acid bacterium fermentation product solution (solid content concentration 2.2%).

Production Example 7 Extract of lotus seeds 100 g of lotus seeds (those with astringent skin removed) were pulverized, and 1900 g of purified water was added to prepare a suspension, which was heated at 80 ° C. for 2 hours. This solution was filtered to obtain 1360 g of a lotus seed extract solution (solid content concentration: 0.9%).

Production Example 8 Lotus seed hydrolyzate 100 g of lotus seed (with the astringent skin removed) was crushed, 1900 g of purified water was added to prepare a suspension, and heat sterilized. After adding 1 g of glucoamylase, 1 g of papain and 0.5 g of cellulase to this solution, pH was adjusted to 7.5 after being added and kept at 45 ° C. for 15 hours. This solution was filtered to obtain 1400 g of a lotus seed hydrolyzed solution (solid content concentration 1.9%).

Production Example 9 A lotus seed fermented lotus seed (with astringent skin removed) 100 g was crushed, 1900 g of purified water was added to prepare a suspension, and heat sterilized. After adding 1 g of glucoamylase, 1 g of papain and 0.5 g of cellulase to this suspension, 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum) were inoculated, followed by static culture at 37 ° C. for 3 days under a nitrogen stream. . After completion of the culture, the mixture was sterilized by heating, and the culture solution was filtered to obtain 1460 g of a lotus seed lactic acid bacteria fermented solution (solid content concentration: 3.0%).

Production Example 10 50 g of barley seeds excluding the husk seed extract shell were pulverized, 950 g of purified water was added to prepare a suspension, and the mixture was heated at 80 ° C. for 2 hours. This solution was filtered to obtain 770 g of a barley seed extract solution (solid content concentration 2.1%).

Production Example 11 50 g of barley seeds excluding the hydrolyzed shell of pearl barley were pulverized and 950 g of purified water was added to prepare a suspension, which was heated at 80 ° C. for 2 hours. After adding 0.5 g of glucoamylase and 0.5 g of papain to this solution, the enzyme was digested at 37 ° C. After cooling, this solution was filtered to obtain 765 g of pearl seed hydrolyzate solution (solid content concentration 2.2%).

Production Example 12. 50 g of barley seeds excluding the fermented husk of barley seeds were pulverized, 950 g of purified water was added to prepare a suspension, and heat sterilized. After adding 0.5 g of glucoamylase and 0.5 g of papain to this suspension, 10 ⁷ yeast / mL of yeast (Saccharomyces cerevisiae) was inoculated and left to stand at 37 ° C. for 3 days. After the number of cultures, the mixture was sterilized by heating, cooled to room temperature, and filtered to obtain 500 g of pearl seed fermented product solution (solid content concentration 1.3%).

  As shown in Tables 1 and 2 below, the cosmetic compositions of Examples 1 to 37 containing any three or more of the extracts, hydrolysates and fermented products of Production Examples 1 to 12, and The cosmetic compositions of Examples 38 to 49 were prepared by adding any one or more of the above-described whitening agents, antioxidants, anti-inflammatory agents, and blood circulation promoters to the compositions. Moreover, the composition (comparative example 1) which consists only of the rice extract of manufacture example 1 was prepared as a comparative comparison of the effectiveness mentioned later.

[Table 1]

[Table 2]

[Table 3]

[Table 4]

Below, the preferable formulation example of this invention is shown.
Formulation Example 1 Latex [Component A] Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Composition of Example 1 3.0 (% by weight of composition with respect to 100 parts of emulsion of Formulation Example 1)
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Soy milk lactic acid bacteria fermentation extract 1.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Examples 2-28. Emulsion An emulsion is obtained in the same manner as in Formulation Example 1 except that 3.0 parts of any one of Examples 2 to 28 is used in place of the composition of Example 1 in Component B of Formulation Example 1. It was.

Formulation Examples 29-37. Emulsion An emulsion was obtained in the same manner as in Formulation Example 1 except that 4.0 parts of any one of Examples 29 to 37 were used in place of the composition of Example 1 in Component B of Formulation Example 1. It was.

Formulation examples 38-49. Emulsion In the component B of Formulation Example 1, an emulsion was obtained in the same manner as Formulation Example 1 except that 5.0 parts of any one of Examples 38 to 49 were used instead of the composition of Example 1. It was.

Formulation Example 50. Lotion [Component A] Part Composition of Example 1 3.0 (% by weight of composition with respect to 100 parts of Lotion of Formulation Example 50)
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Fragrance Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Formulation Examples 51-77. Lotion A lotion was obtained in the same manner as in Formulation Example 50 except that 3.0 parts of any one of Examples 2 to 28 was used instead of the composition of Example 1 in component A of Formulation Example 50. It was.

Formulation examples 78-86. Lotion A lotion was obtained in the same manner as in Formulation Example 50 except that 4.0 parts of any one of Examples 29 to 37 was used instead of the composition of Example 1 in component A of Formulation Example 50. It was.

Formulation examples 87-98. Lotion A lotion was obtained in the same manner as in Formulation Example 50 except that 5.0 parts of any one of Examples 38 to 49 were used instead of the composition in Example 1 in the B component of Formulation Example 50. It was.

Formulation Example 99. Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Composition of Example 1 3.0 (% by weight of the composition based on 100 parts of the lotion of the present Formulation Example 100)
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation examples 100-126. Lotion Toner lotion is obtained in the same manner as in Formulation Example 99, except that 3.0 parts of any one of Examples 2 to 28 is used in place of the composition of Example 1 in Component B of Formulation Example 99. It was.

Formulation examples 127-135. Lotion Toner lotion is obtained in the same manner as in Formulation Example 99 except that 4.0 parts of any one of Examples 29 to 37 are used in the component B of Formulation Example 99 instead of the composition of Example 1. It was.

Formulation examples 136-147. Lotion Toner lotion is obtained in the same manner as in Formulation Example 100 except that 5.0 parts of any one of Examples 38 to 49 are used in Component B of Formulation Example 100 instead of the composition of Example 1. It was.

Formulation Example 148. In the component B of the emulsion formulation example 1, instead of using 2.0 parts of L-ascorbic acid-2-glucoside, 1.0 part of L-ascorbic acid-2-glucoside and 1.0 part of arbutin are used. An emulsion was obtained in the same manner.

Formulation Example 150. Emulsion In the same manner as in Formulation Example 1, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. Got.

Formulation Example 151. In the component B of the emulsion formulation example 1, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide, rice bran extract hydrolyzate (solid content concentration: 3.5%) 5.0 A milky lotion was obtained in the same manner as in Formulation Example 1 with the exception of using the part.

Test Example 1 Inhibition of intracellular tyrosinase activity Using the compositions of Examples 1 to 49 below as samples, the tyrosinase inhibitory effect and fibroblast activation effect of each composition were examined.
[Test method]
Cultured B16 mouse melanoma cells were seeded at 8 × 10 ³ cells / well in a 96-well microplate and placed in Eagle's minimum essential medium (MEM) containing 10% calf serum (FBS) under conditions of 37 ° C. and 5% CO ₂ . After pre-culturing for 1 day, each composition of Examples 1 to 49 was added as a sample solution to Eagle MEM containing 10% FBS and cultured for 2 days under the same conditions. Here, the final concentration of each composition used in this test as a solution was 3.0% by weight with respect to 100 parts of the culture medium for each composition of Examples 1-28. If it is each composition of Examples 29-46, it will be 4.0 weight% with respect to 100 parts of the said culture medium, and if it is each composition of Examples 47-49, it will be 5.0 weight%. Prepared. Next, the culture solution is removed, and a surfactant (Triton X-100) and 5 mM L-dopa solution are added and reacted at 37 ° C. Then, a microplate reader (Model 450, manufactured by Bio-Rad) is used. The dopa value was measured at a wavelength of 490 nm. In the case of no sample addition (control), the same operation as described above was performed, and the relative value of the dopa value at the time of each sample addition with respect to the obtained dopa value was determined and used as the tyrosinase activity rate (%). For comparison, the same test was performed when 3 mM arbutin was added instead of the sample solution (positive control). In addition, the same test was done also about the comparative sample 1 (The final concentration as a solution is 3.0 weight% with respect to 100 parts of culture media) which consists only of the rice extract of manufacture example 1 as a comparison control.

Test Example 2 Fibroblast activation effect [Test method]
Human dermis-derived fibroblasts were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% FCS-containing minimum essential medium, pre-cultured at 37 ° C. for 1 day, and then Examples 1-49 Each composition was added as a sample solution and cultured at 37 ° C. for another 6 days. Here, the final concentration of each composition used in this test as a solution was 3.0% by weight with respect to 100 parts of the culture medium for each composition of Examples 1-28. If it is each composition of Examples 29-46, it will be 4.0 weight% with respect to 100 parts of the said culture medium, and if it is each composition of Examples 47-49, it will be 5.0 weight%. Prepared. Next, 0.2% MTT was added to the cell treatment solution to which the medium was removed and the surfactant (TRITON X-100) was added, and the mixture was kept at 37 ° C., and then a microplate reader (Model 1450, Bio-Rad). MTT value was measured at a wavelength of 370 to 530 nm. In the case where no sample was added (control), the same operation as described above was performed, and the relative value of the MTT value at the time of adding each sample to the MTT value obtained here was determined to obtain the fibroblast MTT activity rate (%). . For comparison, the same test was performed when 50 mM glucose was added instead of the sample solution (positive control). Further, as a comparative control, the same test was performed on Comparative Sample 1 (only the concentration of 3.0% by weight as a solution with respect to 100 parts of the medium) consisting only of the rice extract of Production Example 1.

Tables 5 and 6 show the test results of the intracellular tyrosinase activity effect and the fibroblast activation effect of each composition of Examples 1 to 49.

[Table 5]

[Table 6]

As described above, it was confirmed that any of the compositions of Examples 1 to 49 exhibited markedly superior intracellular tyrosinase activity inhibitory effect and fibroblast activation effect as compared with Comparative Sample 1. These compositions have been shown to exhibit excellent whitening and skin beautifying effects without damaging skin cells. In addition, since the positive controls arbutin and glucose also showed the intracellular tyrosinase activity inhibitory effect and the fibroblast activation effect, it was also confirmed that this test system was performed normally.

Test Example 3 Panel test (skin elasticity, gloss, dullness, spots, freckles, wrinkles, feeling of use)
Regarding cosmetics (milky lotion) containing the cosmetic composition of the present invention, skin firmness, luster, dullness, spots, freckles, wrinkles and feeling of use were evaluated by an actual use test using a monitor.

[sample]
In Test Example 3, the following essence was used as a sample.
(1) Invention sample 1
[Ingredients] part ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Composition of Example 3 3.0
Citric acid 0.3
Sodium citrate 0.6
2. Amount of purified water to 100 parts (2) Sample of the present invention
The essence which mix | blended the composition of Example 6 instead of the composition of Example 1 in the said this invention sample 1. FIG.
(3) Invention sample 3.
The essence which mix | blended the composition of Example 11 in the said invention sample 1 instead of the composition of Example 1. FIG.
(4) Invention sample 4.
The essence which mix | blended the composition of Example 20 instead of the composition of Example 1 in the said this invention sample 1. FIG.
(5) Invention sample 5.
The essence which mix | blended the composition of Example 31 instead of the composition of Example 1 in the said this invention sample 1. FIG.
(6) Sample of the present invention
The essence which mix | blended the composition of Example 49 instead of the composition of Example 1 in the said this invention sample 1. FIG.
(7) Comparative sample
The essence which mix | blended the composition of the comparative example 1 instead of the composition of the example 1 in the said this invention sample 1. FIG.
[Test method]
Using 120 women aged 18 to 50 randomly extracted as subjects, any one of the inventive samples 1 to 6 and the comparative sample 1 were placed on the left and right cheeks respectively of the inventive example or the comparative example. When the emulsion was applied twice a day (morning and evening) for 1 month, the feeling of use and the skin condition were evaluated for each of the following items 1 to 4. For skin firmness, gloss, dullness, spots, and freckles, A: improved, B: slightly improved, C: unchanged, D: slightly worsened, E: slightly worsened, E: worsened Each went. In addition, the feeling of use is A: very good, B: good, C: normal, and D: slightly bad based on the feeling taken by the hand, the elongation during application, the smoothness during application, and the feeling after application. , E: Each was performed by a five-step evaluation of bad.

[result]
Table 7 shows the evaluation results.
[Table 7]
As described above, each of the inventive samples 1 to 6 had a higher effect of improving skin firmness, gloss, dullness, spots, freckles, and fine wrinkles as compared with Comparative Sample 1. Furthermore, the inventive samples 1 to 6 also had a higher feeling of use feeling than the comparative sample 1.

From the above results, the composition of the present invention is remarkably based on the components of three or more kinds of plants among the rice, Brassicaceae Brassica genus, Lilyaceae lotus genus, and pearl barley included in these compositions. Since it exhibits excellent whitening and skin-beautifying effects and is excellent in use feeling, it is useful as a compounding ingredient for cosmetics for improving skin firmness, gloss, dullness, spots, freckles, and fine wrinkles.

Claims (2)

A cosmetic comprising three or more plant components of rice, Brassica plant, Nympaeaceae lotus (Nelumbo) plant and pearl barley,
The plant component is an extract of the plant, a hydrolyzate of the extract, or a fermented product of the plant. The cosmetic according to claim 1, further comprising at least one of a whitening agent, a humectant, a cell activator, an anti-inflammatory agent, an antioxidant, and a blood circulation promoter.