KR20120044583A - Method for boosting antioxidant activity in aloe extract using irradiation - Google Patents

Abstract

PURPOSE: An antioxidant activity enhancement method for an aloe extract using the radial ray radiation is provided to enable users to usefully apply the aloe extract for health food or a cosmetic composition. CONSTITUTION: An antioxidant activity enhancement method for an aloe extract using the radial ray radiation comprises a step of radiating radial rays to the aloe extract in the dose of 1-100kGy. The aloe extract is obtained by extracting dried aloe powder using water, alcohol, or their mixture as a solvent. The alcohol is C1-C4 lower class alcohol. The radial rays are select from gamma rays, electron rays, ultra-violet rays, and X-rays.

Description

Method for boosting antioxidant activity in Aloe extract using irradiation

The present invention relates to a method for enhancing the antioxidant activity of aloe extract using irradiation.

Free radicals are oxygen that has an unstable state of ions and binds to other substances to stabilize, that is, has a strong reactivity, which is a byproduct of various oxidation reactions in the metabolism of normal cells in the human body. Free radicals are produced by phagocytes and play a positive role in controlling infection, while ionizing radiation, ultraviolet rays, environmental pollution, and severe exercise can be used to attack biological tissues, causing inflammatory diseases, cancer, liver failure, and arteriosclerosis. It is known as one of the causes of many diseases, such as gastritis and ultimately a cause of aging. In order to protect biological tissues from the damage of free radicals, antioxidants that convert the free radicals that are already present into a harmless form before reacting with other molecules and inhibit the formation of free free radicals from other molecules are commonly used (Pak Kwang Kyun, Oral Biochemistry Seoul: Gunja Publishing Co., 1999, 138-25), BHA (Butylated Hydroxy Anisole), BHT (Butylated Hydroxy Toluene), α-Tocopherol, Ascorbic Acid, Antioxidants and active oxygen scavengers such as cysteine and glutathione are known.

However, since such antioxidants are chemically synthesized products, there is a problem in that the safety of the human body remains when a large amount of these are used. In addition, these antioxidants are oxidized through the process of reducing the counterpart to produce by-product oxides, in which case, by-products are concerned about the stability of the by-products to the human body. On the other hand, since natural antioxidants have no safety concerns, studies on natural antioxidants have been actively conducted.

Aloe is a tropical or subtropical perennial plant belonging to the Liliaceae family, and is cultivated all over the world, with only 300 to 360 species. The dominant varieties in Korea are Aloe vera and Aloe avoresence.

Aloe gel is 99% water, and nutritional nutrients include mannose, glucose, polysaccharides, glutamic acid, arginine, asparagine and amino acids such as K, Na And minerals such as Ca and the like.

Bioactive substances reported at the current society include phenolic compounds such as aloe emodine, barbaloin and aloesin, sterols and terpenoids, and antioxidants. It is known to have activity, antibacterial action, anti-tumor action, bactericidal action, anti-inflammatory action, tissue formation action, etc. It has been used as a folk remedy for the treatment of burn, skin disease and digestive system disease. (Doo-Ho shin, Korean J. Food & Nutr, 2007, 20, 4: 399-405).

However, the antioxidant activity of aloe is 2 times lower than that of trolox, a water soluble vitamin E analog that prevents lipid peroxidation, and the synthetic antioxidant butylated hydroxytoluene (BHT) is butylated. hydroxytoluene), which is equivalent to α-tocopherol, which is about 1.5 times higher than that of α-tocopherol, but the aloe pulp ethanol extract is 5 times lower than trolox, 2.5 times lower than BHT and about 2 times lower than α-tocopherol. Has been reported (Qiuhui Hu, food chemistry, 2005, 91, 85-90).

Thus, the inventors of the present invention, while investigating a method for increasing the antioxidant activity of aloe, confirmed that the radiation activity of the aloe extract can be enhanced by two times or more, and completed the present invention.

An object of the present invention is to provide a method for enhancing the antioxidant activity of aloe extract using irradiation.

Another object of the present invention to provide an aloe extract with enhanced antioxidant activity prepared by the above method.

Another object of the present invention to provide an antioxidant pharmaceutical composition containing the aloe extract with enhanced antioxidant activity as an active ingredient.

Another object of the present invention is to provide an antioxidant health food composition containing the aloe extract with enhanced antioxidant activity as an active ingredient.

Still another object of the present invention is to provide an antioxidant cosmetic composition containing the aloe extract having enhanced antioxidant activity as an active ingredient.

In order to achieve the above object, the present invention provides a method for enhancing the antioxidant activity of aloe extract using irradiation.

The present invention also provides an aloe extract with enhanced antioxidant activity prepared by the above method.

Furthermore, the present invention provides a pharmaceutical composition for antioxidant containing the aloe extract with enhanced antioxidant activity as an active ingredient.

In addition, the present invention provides a health food composition for antioxidant containing the aloe extract with the enhanced antioxidant activity as an active ingredient.

Furthermore, the present invention provides an antioxidant cosmetic composition containing the aloe extract with enhanced antioxidant activity as an active ingredient.

The aloe extract irradiated with radiation of the present invention may exhibit an effective antioxidant effect in a small amount by more than two times the antioxidant activity compared to the extract not irradiated with radiation, and may be usefully used as a pharmaceutical, health food or cosmetic composition. .

1 is a view showing the color change of the aloe extract by irradiation according to an embodiment of the present invention.
2 is a graph of high performance liquid chromatography (HPLC) analysis according to irradiation according to an embodiment of the present invention.
3 is a graph showing the DPPH radical scavenging activity of the aloe extract by irradiation according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a method of enhancing the antioxidant activity of the aloe extract using radiation comprising the step of irradiating the aloe extract with radiation.

In the present invention, the preparation method of the aloe extract is specifically,

It is preferably prepared by a manufacturing method including the following steps, but not always limited thereto:

Extracting by adding an extraction solvent to aloe (step 1);

Filtering the extract of step 1 (step 2); And

Concentrating the filtered extract of step 2 under reduced pressure (step 3).

In the above method, the aloe of step 1 can be used without limitation, such as cultivated or commercially available. It is preferable to use aloe gel after removing the aloe shell.

In addition, the aloe extract according to the present invention can be prepared as follows. After grinding the aloe gel, it is first filtered using gauze, and then lyophilized for about a week to prepare a powder.

The aloe gel powder was placed in an extraction container, and extracted with a solvent for a predetermined time to obtain an aloe extract. Then, the solids were removed using a filter paper, the suspension was centrifuged, and the supernatant was filtered under reduced pressure. Can be.

The extraction solvent is preferably a solvent selected from water, an alcohol or a mixture thereof, preferably a C ₁ to C ₄ lower alcohol or a mixed solvent thereof, more preferably 40% ethanol.

The amount of the extraction solvent is 2 to 10 times the dry weight of aloe. The extraction method may be an extraction method such as hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction, preferably may be extracted from 1 to 5 times by the immersion extraction method.

The temperature at the time of extraction is preferably room temperature, the extraction time is 1 to 5 days, preferably 3 days.

In the above method, the decompression concentration of Step 3 preferably uses a vacuum decompression concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, the drying is preferably reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

In addition, the radiation to the aloe extract is preferably used gamma rays, electron beams, UV and X-rays, more preferably gamma rays may be used.

The gamma ray is preferably emitted from radioactive isotopes of cobalt (Co) -60, krypton (Kr) -85, strontium (Sr) -90, and cesium (Cs) -137, more preferably cobalt (Co)- 60 radioactive isotopes.

The radiation is preferably irradiated at a dose of 1 to 100 kGy, more preferably at a dose of 30 to 90 kGy.

According to the present invention, irradiated aloe extract according to the DPPH radical scavenging activity experiment was confirmed that the antioxidant activity is increased depending on the dose and the anti-oxidation activity increased more than 2 times compared to before irradiation when irradiated at 30 kGy or more (See Figure 3).

Thus, the aloe extract with enhanced antioxidant activity can be obtained from the method of the present invention.

Furthermore, the present invention provides a pharmaceutical composition for antioxidant containing aloe extract, which is irradiated with radiation and has enhanced antioxidant activity as an active ingredient.

In another aspect, the present invention provides a dietary supplement for antioxidants containing aloe extract, which is irradiated with radiation to enhance the antioxidant activity as an active ingredient.

Furthermore, the present invention provides a cosmetic composition for antioxidant containing aloe extract, which is irradiated with radiation and has enhanced antioxidant activity as an active ingredient.

In a specific embodiment of the present invention, the antioxidant activity-enhanced aloe extract prepared by the method of the present invention exhibited an antioxidant activity more than twice as high as before irradiation (see FIG. 3), thereby preventing the pharmaceutical composition for antioxidant, health It can be usefully used as a food composition or a cosmetic composition.

When the present invention is used in a pharmaceutical composition, it may contain one or more active ingredients exhibiting the same or similar functions in addition to the aloe extracts with enhanced antioxidant activity of the present invention.

The composition of the present invention may be administered in various oral and parenteral dosage forms during actual clinical administration, and when formulated, diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc., which are commonly used, may be used. Are prepared using. Solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, which solid preparations contain at least one excipient such as starch, calcium carbonate, gelatin and the like in aloe extracts with enhanced antioxidant activity. Mix and quiet. Liquid preparations for oral administration include suspensions, solutions, emulsions, and syrups, and may include various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, and lyophilized preparations. As the non-aqueous solvent and suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used.

Dosage ranges according to the patient's worm, age, sex, health condition, diet, time of administration, method of administration, rate of excretion and the severity of the disease. The daily dose of the aloe extract irradiated with radiation according to the present invention is about 0.1 to 100 mg / kg, preferably 5 to 50 mg / kg, and more preferably administered once to several times a day.

When the present invention is used in a health food composition, the antioxidant activity of the present invention may be added as it is, or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method. The blending amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or hygiene). In general, the aloe extract of the antioxidant activity of the present invention in the manufacture of food or beverage is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

There is no particular limitation on the kind of food. Examples of foods to which the hot water extract of the present invention or the hot water extract and its polysaccharide fraction may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream Products, various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes, and includes all healthy foods in the usual sense.

The health food of the present invention may contain various flavors or natural carbohydrates as additional ingredients. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is preferably selected in the range of 0.01 to 0.04 parts by weight, preferably about 0.02 to 0.03 parts by weight, per 100 parts by weight of the health food of the present invention.

In addition to the above, the health food of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols. And carbonation agents used in carbonated beverages. In addition, the health food of the present invention may contain a flesh for producing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The ratio of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health food of the present invention.

In addition, when the present invention is used in the cosmetic composition, the aloe extract with enhanced antioxidant activity of the present invention is contained 0.005 to 50% by weight based on the total weight of the composition, but preferably 1 to 15 parts by weight, more preferably 2 Or it may be added in an amount of 10 parts by weight, and may be used in the above or below the range according to the intended use in the range that has an antioxidant, skin whitening and wrinkle improvement effect and no toxicity. The cosmetic composition of the present invention may contain one or more active ingredients exhibiting the same or similar functions in addition to the aloe extract with enhanced antioxidant activity.

Cosmetics prepared with the cosmetic composition of the present invention can be prepared in the form of general emulsion formulations and solubilized formulations. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the cosmetic composition of the present invention by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be topically or systemically applied commonly used in the field of dermatology. Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to aloe extracts with enhanced antioxidant activity, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances , Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological arts, such as any other ingredients commonly used in the art. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following Examples are only illustrative of the present invention specifically, and are not limited by the Examples of the present invention.

< Manufacturing example > Preparation of Aloe Extracts

The aloe was purchased and used at Gochang Jeju Farm. After removing the aloe shell, the aloe gel was finely ground using a blender, and the finely ground aloe gel was first filtered using gauze and then lyophilized Prepared in the form of a powder.

2 g of the aloe powder prepared above was immersed in 100 ml of 40% ethanol, extracted for 24 hours, and filtered through a filter paper. The concentration of the filtrate was 1.6 mg / ml.

< Example  1> Irradiation on Aloe Extract

The Aloe filtrate extracted from the above preparation was packed in a container with 1.6 mg / ml, and then the source of 100,000 Ci cobalt-60 gamma-irradiation facility (Point source AECL, IR-79, MDS Nordion), which is stored in the Jeongeup Radiation Research Institute of the Korea Atomic Energy Research Institute. International Co. Ltd., Ottawa, On, Canada) were used to examine 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 kGy at room temperature, respectively.

As a result, as shown in Figure 1, it was visually confirmed that the color of the aloe extract is changed from 10 to 40 kGy to red by gamma-irradiation dose, and when 50 to 100 kGy was irradiated, red color was removed again. It was.

< Example  2> after irradiation HPLC Secondary using Metabolite  Change analysis

Secondary metabolites of the irradiated extract of Example 1 were measured using Agilent HPLC (Agilent Technological, USA). The column used YMC-Pack Pro C18 (250 × 4.6 mml. D. S-5 μm, 12 nm) at which time the temperature of the column was adjusted to 40 ° C. HPLC was performed with 100% water (A) and 100% acetonitrile solution (B) as the mobile phase.

The mobile phase conditions are shown in Table 1 and maintained at a flow rate of 0.2 ml / min for 120 minutes. LC-MS analysis of materials separated from the column was performed using a quadrupole mass spectrometer detector (HP1100LC-MSD, Agilent Technologies, Canada). Secondary metabolites were measured at 220 nm.

The change in the secondary metabolite by radiation was used as the area value of the main peak, and the relative value was obtained with the control group as 100%.

Mobile phase       A: water B: acetonitrile

Extraction Mode Travel time (minutes) % Mobile phase B 0 16 24 16 72 33 100 60 120 100

As a result, aloe extracts typically have peaks of 12.510, 29.374, 86.036 and 89.424 minutes. In particular, after irradiating gamma rays at 30 kGy, new substances with peaks of 15.194 minutes began to be produced. Investigations confirmed that 15.194 minute peaks were produced at equivalent levels (see FIG. 2).

< Example  3> of aloe extract after irradiation DPPH  Measurement of inhibitory activity

DPPH (1,1-diphenyl-2-picryhydrazyl) receives electrons or hydrogen from sulfated materials to form irreversibly stable molecules, so it can measure antioxidant activity from electron donating ability. At this time, DPPH's inherent blue-blue color is thinned and the color difference is measured by colorimetric measurement of electron donating ability (Jeon et. al . , J. Kor . Soc . Food sic . Nutr . 38 (1): 1-8, 2009).

500 μl of 0.2 mM DPPH was added to 500 μl of aloe extract irradiated with 1 mg / ml gamma ray and aloe extract irradiated with gamma ray, respectively, and reacted at room temperature for 30 minutes, and then absorbance was measured at 517 nm. Ascorbic acid was used as a positive control, and 40% ethanol extractant was used as a negative control. DPPH inhibitory activity was expressed as a decrease in absorbance of the sample solution addition group and no addition group according to Equation 1.

As a result, ascorbic acid, a positive control group, showed 96.5% activity at the concentration of 0.01 mg / ml, and the unirradiated aloe extract (1.6 mg / ml) showed 53.1% activity. In the case of, the antioxidant activity was increased to 63.8 to 94.8% as the gamma-irradiation dose was increased, in particular, it was confirmed that more than 90% of the activity appeared in 30 to 100 kGy.

Therefore, irradiation with aloe extract enhances antioxidant activity, so the aloe extract irradiated with radiation of the present invention may be usefully used as a medicine, health food, cosmetic composition, and the like.

< Formulation example  1> Preparation of Pharmaceutical Formulations

1. Preparation of tablets

Tablets containing the aloe extract irradiated with radiation of the present invention as an active ingredient were prepared by the following method.

The extract of the above example was mixed with lactose, starch and pregelatinized corn starch, and then a suitable volume of purified water was added and granulated into a powder. The granules were dried and then mixed with magnesium stearate and compressed to obtain tablets.

The components of the tablet are as follows.

Irradiated Aloe Extract 5.0 mg

Lactose BP 150.0 mg

Starch BP 30.0 mg

Pregelatinized Corn Starch BP 15.0 mg

1.0 mg magnesium stearate

2. Preparation of Capsule

Capsules containing aloe extract irradiated with the present invention as an active ingredient were prepared by the following method.

The compound of the above example was mixed with an excipient and magnesium stearate. The resulting mixture was filled into gelatin capsules to obtain capsules.

The components of the capsule are as follows.

Irradiated Aloe Extract 5.0 mg

Starch 1500 100.0 mg

Magnesium Stearate BP 1.0 mg

3. Preparation of Injection

Injection solution containing aloe extract irradiated with radiation of the present invention as an active ingredient was prepared by the following method.

Dissolve the irradiated aloe extract in a suitable volume of injectable sodium chloride BP, adjust the pH of the resulting solution to pH 3.5 with dilute hydrochloric acid BP, then adjust the volume with injectable sodium chloride BP and sufficiently Mixed. The solution was filled into a 5 ml Type I ampoule made of transparent glass, and the glass was dissolved and enclosed under an upper grid of air, followed by autoclaving at 120 ° C. for at least 15 minutes to obtain an injection solution.

The components of the injection solution are as follows.

100 ㎍ / ml Irradiated Aloe Extract

Dilute hydrochloric acid BP until pH 3.5

Injectable sodium chloride BP up to 1 ml

< Formulation example  2> Irradiated Aloe Extract of  Production of functional foods to contain

The present inventors prepared the food containing the aloe extract irradiated as an active ingredient as follows.

1. Manufacture of beverage

522 mg of honey

Chioctosanamide 5 mg

Nicotinamide 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Irradiated Aloe Extract 0.48? 1.28 mg

200 ml of water

A beverage was prepared using the above-mentioned composition and content by a conventional method.

2. Of chewing gum  Produce

Gum base 20%

Sugar 76.36? 76.76%

Irradiated Aloe Extract 0.24? 0.64%

1% fruit flavor

Water 2%

Chewing gum was prepared using the above-mentioned composition and content by a conventional method.

3. Manufacture of Candy

50 sugar 60%

Starch syrup 39.26? 49.66%

Irradiated Aloe Extract 0.24? 0.64%

Orange flavor 0.1%

The composition and the content of the candy were prepared using a conventional method.

4. Biscuit  Produce

Force Class 1 88 kg

Gravity First Class 76.4 ㎏

16.5 kg per white

2.5 kg of salt

2.7 kg of glucose

Palm shortening 40.5 kg

5.3 kg of ammo

Medium kg 0.6 kg

0.55 kg sodium bisulfite

Rice flour 5.0 kg

Vitamin B1 0.003 kg

0.003 kg of vitamin B2

Milk Flavor 0.16 ㎏

71.1 kg of water

Whole milk powder 4 kg

Substitute powder 1 kg

0.1 kg of calcium phosphate

Spray salt 1 kg

25 kg of spray oil

Irradiated Aloe Extract 0.2? 0.5 kg

Biscuits were prepared using conventional methods with the above composition and content.

5. Manufacture of Ice Cream

Milkfat 10.0%

Non-fat solids 10.8%

Sugar 12.0%

Starch syrup 3.0%

Emulsifying stabilizer (span) 0.5%

Spices (Strawberries) 0.15%

Water 63.31? 62.91%

Irradiated Aloe Extract 0.24? 0.64%

Ice cream was prepared using conventional methods using the above composition and content.

6. Chocolate  Produce

Sugar 34.36? 34.76%

Cocoa Butter 34%

Cocoa Mass 15%

Cocoa Powder 15%

Lecithin 0.5%

0.5% vanilla

Irradiated Aloe Extract 0.24? 0.64%

With the above composition and content, chocolate was prepared using conventional methods.

< Formulation example  3> Preparation of external preparation for skin

1. Preparation of Cream

Cetostearyl alcohol 2.8 parts by weight

2.6 parts by weight of beeswax

Stearic acid 1.4 parts by weight

2 parts by weight of glycerol phosphate monostearate

Fiji-100 Stearate 1 part by weight

Sesquioleate sorbitan 1.4 parts by weight

4 parts by weight of jojoba oil

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

2 parts by weight of macadamia oil

Tocopherol acetate 0.2 parts by weight

0.4 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methyl paraben 0.05 parts by weight

Glycerin 6 parts by weight

0.2 parts by weight of d-pandenol

Irradiated Aloe Extract 4.6 parts by weight

0.2 parts by weight of triethanolamine

pt 41891 0.2 parts by weight

pH ₂ O 46.05 parts by weight

2. Preparation of Lotion

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

       Allantoin 0.1 part by weight

Irradiated Aloe Extract 3.5 parts by weight

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

pH ₂ 0 48.3 parts by weight

Claims (13)

A method of enhancing the antioxidant activity of the aloe extract comprising the step of irradiating the aloe extract.
The method of claim 1, wherein the aloe extract is a method of extracting dried aloe powder using water, alcohol or a mixture thereof as a solvent.
The method of claim 2 wherein the alcohol is a C ₁ to C ₄ lower alcohol.
The method of claim 1 wherein the radiation is any one selected from the group consisting of gamma rays, electron beams, UV and X-rays.
5. The method of claim 4, wherein said radiation is gamma rays.
The method of claim 5, wherein the gamma rays are emitted from any one radioisotope selected from the group consisting of cobalt (Co) -60, krypton (Kr) -85, strontium (Sr) -90 and cesium (Cs) -137 Characterized in that the method.
7. The method of claim 6, wherein the gamma rays are emitted from cobalt (Co) -60 radioisotopes.
The method of claim 1, wherein the radiation is irradiated at a dose of 1-100 kGy.
9. A method according to claim 8, wherein the radiation is irradiated at a dose of 30 to 90 kGy.
Aloe extract with enhanced antioxidant activity prepared by the method according to any one of claims 1 to 9.
A pharmaceutical composition for antioxidant containing the aloe extract of claim 10 as an active ingredient.
Health functional food composition for antioxidant containing the aloe extract of claim 10 as an active ingredient.
Antioxidant cosmetic composition containing an aloe extract of claim 10 as an active ingredient.

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