KR101125223B1 - Method for removing the pigment of Centipede grass extract using irradiation - Google Patents

Abstract

The present invention relates to a method for removing a pigment from the centipedes grass extract, preferably the centipedes grass extract is removed by irradiation of gamma rays or electron beams to the centipedes grass extract extracted using methanol, the physiological activity is maintained It relates to a method of manufacturing. According to the method of the present invention, it is possible to remove the pigment from the centipedes grass extract without complicated pigment removal process using the radiation irradiation technology, and to maintain the antioxidant, skin whitening and wrinkle improvement activity similar to before irradiation as an active ingredient of cosmetics It can be usefully used.

Sentipedgrass, pigment removal, secondary metabolites, cosmetics, radiation

Description

Method for removing the pigment of Centipede grass extract using irradiation}

The present invention relates to a method for removing the pigment from the centipedes grass extract using radiation.

Centipede grass is a slow-growing, turbulent grass that is a perennial grass that crawls and forms turf. It belongs to the Poaceae family and its scientific name is Eremochloa ophiuroides (Munro) Hack. It is mainly distributed in China, East Asia, Indochina, the northeastern United States, Meso America, and the Caribbean, and is widely adapted to various soil conditions, but is especially resistant to moist, low acidic soils and low-quality sandy soils. As a morphological feature, the leaves are 15-30 mm long, 2-4 mm wide, flat with a mid vein, hairless except for the collar part, and the leaf ends are round. The stem has a very firm sheath, and the roots are thin branched stolons. The flower part has one raceme of about 3 to 5 inches, which has a purple, slightly flat, small spikelet arranged in two rows. Have Its main use is as a lawn or turf grass and as a forage due to its high leafiness and good taste. The centipedegrass produces useful secondary metabolites such as isoorientin, isoorientin, rhamnosylisoorientin and maysin, as shown in FIG. 1, and as shown in FIG. nidin -3- O - also it produced the dye, such as glucoside (Cyanidin-3-O -glucoside) and the end of neutravidin 3- (6-acetonitrile-yl) glucoside [Malvidin-3- (6-acetoyl ) glucoside].

The centifedgrass has cyanidin-3- O -glucoside and malvidin3- (6-acetoyl) glucose when used as a cosmetic composition by having physiological activities such as antioxidant activity, whitening effect and antiwrinkle activity. There is a problem of low functionality due to the high content of pigments such as seeds. Although a radiation method or the like is known as a method of removing the pigment, there is a problem that the physiological activities can be removed by the radiation.

However, contrary to the expectation, the present inventors have found that the centipedes grass extract having antioxidant activity, whitening effect and anti-wrinkle activity can be successfully removed from the pigment while maintaining the physiological activity. The present invention was completed by confirming that it can be usefully used.

It is an object of the present invention to provide a method for preparing a centipede glass extract in which the dye including the step of irradiating the centipedes grass extract is removed, the biological activity is maintained.

Another object of the present invention is to provide a centipedegrass extract prepared by the above method, the pigment is removed, the physiological activity is maintained.

Another object of the present invention is to provide a cosmetic composition for antioxidant, skin whitening and anti-wrinkle prepared by the above method, the pigment is removed, containing the centipede glass extract with physiological activity as an active ingredient.

Another object of the present invention is to provide a dietary supplement for antioxidant, skin whitening and anti-wrinkle prepared by the above method, which contains the centipedegrass extract, in which the pigment is removed and the physiological activity is maintained as an active ingredient.

In order to achieve the above object, the present invention provides a method for producing a centipede glass extract, the pigment is removed, including the step of irradiating the centipede glass extract radiation, the physiological activity is maintained.

The present invention also provides a centipedegrass extract prepared by the above method, the pigment is removed, the physiological activity is maintained.

In another aspect, the present invention provides a cosmetic composition for antioxidant, skin whitening and anti-wrinkle prepared by the above method, the pigment is removed, containing the centipede glass extract physiological activity is maintained as an active ingredient.

In addition, the present invention provides a health functional food for antioxidant, skin whitening and wrinkle improvement, which is prepared by the above method, contains the centipede glass extract, in which the pigment is removed and the physiological activity is maintained as an active ingredient.

According to the method of the present invention, it is possible to remove the pigment from the centipedes grass extract without complicated pigment removal process using the radiation irradiation technology, and to maintain the antioxidant, skin whitening and wrinkle improvement activity similar to before irradiation as an active ingredient of cosmetics It can be usefully used.

Hereinafter, the term used by this invention is demonstrated.

The present invention provides a method for preparing a centipede glass extract in which the dye including the step of irradiating the centipede glass extract is removed, the physiological activity is maintained.

The centipedes grass extract is preferably prepared by a manufacturing method comprising the following steps, but not always limited thereto:

1) extracting by adding an extracting solvent to centipede glass;

2) cooling the extract of step 1) and filtering; And

3) concentrating the filtered extract of step 2) under reduced pressure.

In the above method, the centipedes of step 1) can be used without limitation, such as those grown or commercially available. The centifed grass is preferably used leaves.

In addition, the centipede glass extract according to the present invention may be prepared as follows. Centipedgrass is washed thoroughly with water and then dried for 7 days at shade and room temperature. After crushing the dried centipedes grass, it is placed in an extraction container, and extracted with a solvent for a predetermined time at an appropriate temperature. After the centipedes grass extract is obtained, solids may be removed using a filter paper, the suspension may be centrifuged, and the supernatant may be filtered under reduced pressure. The extraction solvent is preferably a solvent selected from water, an alcohol or a mixture thereof, preferably a lower alcohol of C ₁ to C ₄ or a mixed solvent thereof, more preferably 100% ethanol, but is not limited thereto. It doesn't happen. The amount of the extraction solvent is 2 to 10 times the dry weight of centifed glass. The extraction method may be an extraction method such as hot water extraction, immersion extraction, reflux cooling extraction and ultrasonic extraction, preferably may be extracted from 1 to 5 times by the immersion extraction method. The temperature at the time of extraction is preferably room temperature, the extraction time is 1 to 5 days, preferably 3 days.

In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in step 3), but it is not limited thereto. In addition, the drying is preferably reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but is not limited thereto.

The pigment is cyanidin -3- O - one containing the glucoside (Cyanidin-3-O -glucoside) and the end of neutravidin 3- (6-acetonitrile-yl) glucoside [Malvidin-3- (6-acetoyl ) glucoside], It is not limited to this.

The physiological activity exhibits antioxidant activity, skin whitening effect and wrinkle improvement effect, and is characterized in that the physiological activity is maintained as compared with before irradiation.

The radiation gamma rays, electron beams or X-rays can all be used, and gamma rays or electron beams are preferably used. The gamma rays are preferably irradiated with gamma rays emitted from radioactive isotopes such as cobalt (Co) -60, krypton (Kr) -85, strontium (Sr) -90, or cesium (Cs) -137. More preferably, but not limited to, emission from Co) -60 radioisotopes. The irradiation dose is expected to be greater pigment removal effect as the irradiation dose is larger, as shown in Figure 5, the preferred range that can exhibit the desired effect is 1-100 kGy, more preferably 2-50 kGy, 4 Most preferred is -20 kGy. Range of the irradiation dose is cyanidin -3- O - glucoside (Cyanidin-3-O -glucoside) and the end of neutravidin 3- (6-acetonitrile-yl) glucoside [Malvidin-3- (6-acetoyl ) glucoside] It was set on the basis that the pigment such as decreases.

In a specific embodiment of the present invention, as a result of irradiating gamma rays or electron beams to the centipedes grass extract extracted with 100% methanol, cyanidin-3- O -glucoside and malvidin 3- (6-acetoyl) depending on the dose. It was confirmed that the amount of pigments such as glucoside and the like is reduced (see FIGS. 3 to 5). At this time, the amount of useful secondary metabolites, such as isoorientin, isoorientin, rhamnosylisoorientin, and maysin, showed a similar level of content as before irradiation (see FIG. 6). Thus, the method of the present invention can be usefully used to remove the pigment from the centipedes grass extract.

The present invention also provides a centipedegrass extract prepared by the above method, the pigment is removed, the physiological activity is maintained.

In a specific embodiment of the present invention, as a result of irradiating gamma rays or electron beams to the centipedes grass extract extracted with 100% methanol, cyanidin-3- O -glucoside and malvidin 3- (6-acetoyl) depending on the dose. It was confirmed that the amount of pigments such as glucoside and the like is reduced (see FIGS. 3 to 5). At this time, the amount of useful secondary metabolites such as iso-orientin, rhamnosyliso-orientin, and mayin showed a similar level of content before irradiation (see FIG. 6). Thus, the centipedes grass extract was obtained without the complex pigment removal process, the biological activity was maintained from the method of the present invention.

In another aspect, the present invention provides a cosmetic composition for antioxidant, skin whitening and anti-wrinkle prepared by the above method, the pigment is removed, containing the centipede glass extract physiological activity is maintained as an active ingredient.

In a specific embodiment of the present invention, the centipedegrass extract with the pigment removed and the physiological activity maintained by the method of the present invention exhibits the same level of antioxidant activity, whitening effect and wrinkle improvement effect as before irradiation for pigment removal. (See FIGS. 7 to 9), it can be usefully used as a cosmetic composition for antioxidant, skin lightening and wrinkle improvement.

Although the pigment is removed from the cosmetic composition of the present invention and the physiological activity is maintained, the centifedgrass extract is contained in an amount of 0.005 to 50% by weight based on the total weight of the composition, but preferably 1 to 15 parts by weight, more preferably 2 or It may be added in an amount of 10 parts by weight, and may be used in the range above or below the range according to the use purpose in the range of anti-oxidation, skin lightening and anti-wrinkle effect and no toxicity. The cosmetic composition of the present invention may contain one or more active ingredients exhibiting the same or similar function in addition to the centipedes extract, the pigment is removed, the biological activity is maintained.

Cosmetics prepared with the cosmetic composition of the present invention can be prepared in the form of general emulsion formulations and solubilized formulations. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the cosmetic composition of the present invention by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be topically or systemically applied commonly used in the field of dermatology. Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the centipede grass extract, the pigment is removed, the physiological activity is maintained in addition to fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, Foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or hydrophilic It may contain adjuvants commonly used in the field of cosmetics or dermatology, such as oil-based actives, lipid vesicles or any other ingredients commonly used in cosmetics. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

In addition, the present invention provides a health functional food for antioxidant, skin whitening and wrinkle improvement, which is prepared by the above method, contains the centipede glass extract, in which the pigment is removed and the physiological activity is maintained as an active ingredient.

The pigment of the present invention is removed and the physiologically active centifedgrass extract can be added as it is or used in combination with other foods or food ingredients, and can be suitably used according to conventional methods. The blending amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or hygiene). Generally, in the preparation of food or beverage, the dye pigment of the present invention is removed and the physiologically active centipedegrass extract is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

There is no particular limitation on the kind of food. Examples of foods to which the hot water extract of the present invention or the hot water extract and its polysaccharide fraction may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream Products, various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes, and includes all healthy foods in the usual sense.

The health food of the present invention may contain various flavors or natural carbohydrates as additional ingredients. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is preferably selected in the range of 0.01 to 0.04 parts by weight, preferably about 0.02 to 0.03 parts by weight, per 100 parts by weight of the health food of the present invention.

In addition to the above, the health food of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols. And carbonation agents used in carbonated beverages. In addition, the health food of the present invention may contain a flesh for producing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination. Although the ratio of such an additive is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health food of the present invention.

Hereinafter, the present invention will be described in detail by examples and production examples.

However, the following Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Preparation Examples.

< Example  1> Sentipedgrass  Irradiation on extract

<1-1> Sentipedgrass  Preparation of Extract

Sentipedgrass seeds from Fukukaen Nursery and Blub co. It was imported from Korea Atomic Energy Research Institute. After adding 100% methanol to the sample and grinding it with a blender, the extract obtained by immersion at room temperature for 3 days was concentrated under reduced pressure at 50 ° C. The sample concentrated under reduced pressure was dissolved in 100% methanol at a concentration of 20 mg / ml.

<1-2> irradiation

Electron beams were used for the Electron-beam processing facility (UELV-10-10S, Russia), and gamma irradiation was performed at room temperature using a 60Co gamma ray irradiation facility (MDS Nordion, Canada) at 1-100 kGy.

As a result, as shown in Figure 3, it was visually confirmed that the pigment of the centipedes grass extract is removed depending on the irradiation amount of gamma rays or electron beams, it was confirmed that the total absorbed dose is completely removed at 15 kGy or more.

<1-3> HPLC  And LC - MS  analysis

Secondary metabolites and pigment contents of the centipedegrass extract of Example 1-1 were measured using Agilent HPLC (Agilent Technological, USA), and also secondary metabolism of the irradiated extract of Examples 1-2 The product and pigment contents were measured. The column used Beckman Coulter ULTRASPHERE (5 μm, 4.6 mm ± 25 cm) at which time the temperature of the column was adjusted to 40 ° C. HPLC was performed using a mobile phase containing a solution containing 0.1% trifluoroacetic acid (TFA) and a solution containing 50% acetonitrile (B) containing 0.1% TFA. Mobile phase conditions are shown in Table 1 and maintained at a flow rate of 1 ml / min for 50 minutes. LC-MS analysis of materials separated from the column was performed using a quadrupole mass spectrometer detector (HP1100LC-MSD, Agilent Technologies, Canada). Secondary metabolites were measured at 360 nm and pigments were measured at 520 nm. The amount of change of the dye due to radiation was used as the area value of the main dye peak, and the relative value was determined using the control as 100%.

Elution method
(Tilt condition) Elution time A: 0.1% TFA B: 50% acetonitrile solution in 0.1% TFA 0 80% 20% 18 67% 33% 30 40% 40% 40 40% 40% 42 80% 20% 50 80% 20%

As a result, as shown in FIG. 1, useful secondary metabolites such as isoorientin, rhamnosylisoorientin, and maysin were identified. The molecular weight of isoorientin is m / z = 449 [M + H] +, the molecular weight of rhamnosylisoorientin is m / z = 595 [M + H] +, and the rhamnose [m / z = 431, [ M + H] +] was confirmed that the binding sugar. It was confirmed that the molecular weight of the mycin was m / z = 577 [M + H] +, and rhamnose [m / z = 431, [M + H] +] was a binding sugar.

In addition, as shown in FIG. 2 cyanidin -3- O - glucoside (Cyanidin-3-O -glucoside) and the end of neutravidin 3- (6-acetonitrile-yl) glucoside [Malvidin-3- (6-acetoyl ) glucoside And other dyes were also confirmed. The molecular weight of cyanidin-3- O -glucoside is m / z = 449 [M + H] + and the molecular weight of malvidin3- (6-acetoyl) glucoside is m / z = 535 [M + H] It confirmed that it is +.

In addition, as a result of examining the pigment content of the irradiated centifed glass extract, as shown in Figure 4, the pigment of the centifed glass is the main pigment by cyanide-3- O -glucoside and malvidin 3 by gamma rays and electron beams. -(6-acetoyl) glucoside was effectively removed. In addition, as shown in FIG. 5, the cyanidin-3- O -glucoside decreased to about 69.8, 18.3, 9.6, and 7.4% compared to the control group as the gamma irradiation dose increased to 2.5, 5, 10, and 20 kGy. By, it decreased to about 68.8, 14.3, 8.6, 7.2%. Malvidin 3- (6-acetoyl) glucoside was reduced to 73.5, 21.4, 9.4 and 7.0% by gamma rays and to 70.5, 20.4, 9.0 and 6.9% by electron beams. However, the content of the secondary metabolite of the centipedes grass extract showed a similar level as before irradiation even after irradiation (FIG. 6).

< Example  2> Sentipedgrass  Determination of Biological Activities of Extracts

<2-1> DPPH  Measurement of inhibitory activity

DPPH (1,1-diphenyl-2-picryhydrazyl) receives electrons or hydrogen from sulfated materials to form irreversibly stable molecules, so it can measure antioxidant activity from electron donating ability. At this time, DPPH's inherent blue-blue color is thinned and the color difference is measured by colorimetric measurement of electron donating ability (Jeon et. al . , J. Kor . Soc . Food sic . Nutr . 38 (1): 1-8, 2009).

400 μl of 0.2 mM DPPH was added to 400 μl of extract (1 mg / ml), followed by reaction at room temperature for 30 minutes, and then absorbance at 517 nm was measured. Ascorbic acid was used as a positive control, and the negative control was measured using a solvent in which the extract was dissolved. DPPH inhibitory activity was expressed as a decrease in absorbance of the sample solution addition group and no addition group according to Equation 1.

As a result, as shown in Figure 7, the antioxidant activity of the centipedes grass extract (1 mg / ㎖) showed a high activity of about 95% irrespective of the irradiation dose of radiation, untreated (0 kGy) Similar activity).

<2-2> tyrosinase ( Tyrosinase ) Inhibition activity measurement

The biosynthetic pathway of the main production process of melanin pigment is produced by polymerization of amino acid and protein via derivatives such as dopaquinone produced by enzymatic action of tyrosinase using tyrosine as a starting material (Lerner AB .. and Fitzpatrick TB, Physiol Rev 30: 91-126, 1950; Pawelek JM and Korner AM, Am Sci 70:... 136-145, 1982). Melanogenesis inhibitory mechanisms include a type that directly inhibits the melanogenesis enzyme tyrosinase enzyme itself and a type that inhibits melanin production in skin pigment cells, although it does not show direct inhibition of tyrosinase isolated from cells. (Kahn V and Andrawis A., Phytochem . 24: 905-908, 1985). Tyrosinase inhibitory activity was determined by Yagi (Yagi A et. al . , Planta medica . 3981: 517-519, 1986).

0.2 ml of mushroom tyrosinase in a mixture of 0.1 ml of extract, 0.5 ml of 0.175 M sodium phosphate buffer (pH 6.8) and 0.2 ml of 10 mM L-DOPA (L-3,4-dihydroxyphenylalanine) substrate solution After reacting for 2 minutes at 25 ℃ by adding mushroom tyrosinase (110 Unit), the DOPA chrome produced in the reaction solution was measured at 475 nm. The conventional skin whitening agent kojic acid was used as a positive control, and the negative control was measured using a solvent in which the extract was dissolved. The tyrosinase inhibitory activity was expressed as the absorbance decrease rate of the sample solution addition group and no addition group according to Equation 1.

As a result, as shown in FIG. 8, the whitening effect of the centipedes grass extract (10 mg / ml) was almost the same as the antioxidant activity. It showed a whitening effect activity.

<2-3> elastase ( Elastase ) Inhibition activity measurement

Porcine pancrease elastase inhibitory activity was measured by using N-succinyl- (L-Ala) 3-p-nitroanilide as a substrate and measuring the production of p-nitroanilide at 405 nm for 20 minutes at 37 ° C. 100 μl of the sample solution was added to 100 μl of an elastase (2.5 unit) solution dissolved in 50 mM Tri-HCl buffer (pH 8.6), followed by 100 μl 0.4 M Tri-HCl (pH 8.6). At the end, add 200 μl of substrate solution [substrate N-succinyl- (L-Ala) 3-p-nitroanilide (0.5 mg / ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6)] After the reaction was adjusted to 500 μl, the reaction was carried out at 37 ° C. for 20 minutes and measured at 405 nm. Ursolic acid was used as a positive control, and a solvent in which an extract was dissolved was used as a negative control. Elastase inhibitory activity was represented by the absorbance reduction rate of the sample solution addition group and no addition group according to Equation 1.

As a result, as shown in FIG. 9, the antiwrinkle effect of the centipedegrass extract (10 mg / ml) was measured. As a result, the activity change by radiation dose was not observed as in the antioxidant activity, and 90% of ursolic acid was observed. Excellent wrinkle improvement effect activity was shown above.

< Manufacturing example  1> Cosmetics  Preparation of the composition

<1-1> preparation of flexible lotion

The pigment of the present invention is removed, the preparation example of the flexible lotion containing the centipede glass extract was maintained as physiological activity as an active ingredient was prepared as shown in Table 2.

Raw material Content (parts by weight) Centipegrass extract, the pigment of the present invention is removed and the biological activity is maintained 10.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<1-2> Preparation of nourishing cream

The pigment of the present invention was removed, and the preparation example of the nourishing cream containing the centipede glass extract was maintained as physiological activity was prepared as shown in Table 3.

Raw material Content (parts by weight) Centipegrass extract, the pigment of the present invention is removed and the biological activity is maintained 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavoring agent Quantity Purified water Balance

Preparation Example 2 Preparation of Food

Foods containing centipedegrass extract from which the pigment of the present invention was removed and the physiological activity was maintained were prepared as follows.

1. Preparation of Cooking Seasonings

The pigment of the present invention was removed, and 20-95 parts by weight of centipedegrass extract, which retained physiological activity, was prepared for cooking for health promotion.

2. Manufacturing of Flour Foods

The pigment of the present invention is removed, and 0.5 to 5.0 parts by weight of the centipedegrass extract, which maintains physiological activity, is added to the flour, and bread, cake, cookies, crackers and noodles are prepared using the mixture to prepare foods for health promotion. It was.

3. Manufacture of dairy products

The pigment of the present invention was removed, and 5-10 parts by weight of the centipedegrass extract, the physiological activity of which was maintained, was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

4. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were alphanated by a known method to distribute the dried ones, and then prepared into a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then ground to a powder having a particle size of 60 mesh.

The grains, seeds and the pigments of the present invention prepared above were removed, and the dry powder of the centipedegrass extract, which retained its physiological activity, was formulated in the following ratio.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Hot water extract of the present invention or the hot water extract and its polysaccharide fraction (3 parts by weight),

(0.5 part by weight),

Foxglove (0.5 part by weight)

< Manufacturing example  3> Manufacturing of beverage

1. Manufacture of health drinks

       1000 mg of centipedegrass extract, in which the pigment of the present invention was removed and physiological activity was maintained

       Citric acid 1000 mg

       100 g oligosaccharides

       Plum concentrate 2 g

       1 g of taurine

       Add 900 ml of purified water

After mixing the above components according to the conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and intended use.

1 is contained in the centipedegrass extract 2 shows the results of analyzing secondary metabolites.

2 is contained in the centipede glass extract The figure which showed the result of analyzing a pigment | dye.

3 to 5 show the results of analyzing the pigment of the centipedes grass extract after irradiation:

3: picture;

4: HPLC graph; And,

5: bar graph.

6 is a diagram showing the results of analyzing the secondary metabolite of the centipedes grass extract after irradiation.

7 to 9 are diagrams showing the results of analyzing the biological activity of the centipedes grass extract after irradiation:

7: DPPH inhibitory activity;

8: tyrosinase inhibitory activity; And,

9: elastase inhibitory activity.

Claims (14)

1) extracting the dried centipedes glass powder with water, C ₁ to C ₄ alcohol or a mixture thereof as a solvent; And 2) step 1), cyanidin, which comprises irradiating gamma-rays or electron beams in the extract obtained in the -3- O - glucoside (Cyanidin-3-O -glucoside), or the end of neutravidin 3- (6-acetonitrile-yl) Glucoside [Malvidin-3- (6-acetoyl) glucoside] A method for preparing centifedgrass extract in which the pigment is removed and the physiological activity is maintained. delete delete delete The method of claim 1, wherein the gamma or electron beam is irradiated at a dose of 1-100 kGy. The method of claim 1, wherein the gamma or electron beam is irradiated at a dose of 2-50 kGy. The method of claim 1, wherein the gamma or electron beam is irradiated at a dose of 4-20 kGy. delete The method of claim 1, wherein the physiological activity is any one selected from the group consisting of antioxidant activity, skin whitening effect and wrinkle improvement effect. delete delete delete delete delete

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