KR102252763B1 - Method of Preparing Extract of Red Ginseng Having a Reduced Chromaticity - Google Patents

Abstract

The present invention relates to a method for preparing a red ginseng extract with reduced chromaticity, and by filtering the red ginseng extract through an ultrafiltration membrane having a predetermined molecular weight cutoff, a red ginseng extract having a reduced chromaticity and enhanced active ingredient can be obtained. Since red ginseng extract contains an active ingredient and its color is reduced, it can be used as a raw material in the manufacture of products such as cosmetics or food.

Description

Method of Preparing Extract of Red Ginseng Having a Reduced Chromaticity {Method of Preparing Extract of Red Ginseng Having a Reduced Chromaticity}

The present invention relates to a method for producing a red ginseng extract with reduced color.

Ginseng is a perennial herb belonging to the genus Panax, and is one of the herbal medicines used in oriental medicine. Red ginseng is produced by applying heat to ginseng, and ginsenosides that do not exist in ginseng are produced in red ginseng. About 40 species of ginsenosides have been discovered so far, and it has been confirmed to have an excellent effect on regulating body functions, that is, normalizing physiological functions, by exerting a wide range of effects on the central nervous system, endocrine system, and metabolic system. These ginsenosides have similar or opposite actions to each other, but it is known that specific ingredients exert various effects either alone or through interactions of several types.

Arginine-fructose-glucose (AFG) is a type of amino sugar and is known to be effective in the treatment of obesity and diabetes because it inhibits the absorption of sugar from the gastrointestinal tract and also suppresses the absorption of fat. In addition, since amino sugars facilitate blood circulation, they are effective in preventing and treating diseases of the blood circulation system such as high blood pressure, arteriosclerosis and stroke. Although it is known that a large amount of amino sugar is produced in the process of steaming (heat treatment) of red ginseng, commercial red ginseng-related products, especially liquid red ginseng extract products, contain almost no amino sugar. Therefore, it is necessary to develop a method for preparing a red ginseng extract containing a high content of amino sugar.

Amino acids are excellent in biocompatibility and environmental compatibility, and are widely used as raw materials for cosmetics. Among the major amino acids, arginine has a guanidine group as a characteristic functional group in its molecule, and has excellent adsorption properties to skin and hair. In addition, it is necessary for the production of epithelial cells, cranial nerve cells, and neutrophils. Up to now, the focus is on the research on the extraction method containing arginine in a high content, and the research on the processing method for enhancing arginine from the extract is insufficient.

Since natural product extracts contain a large amount of strong natural pigments such as chlorophyll, flavonoids, and carotenoids, there is a problem that the color of the existing product changes when the product is added to it. And there are many limitations in application to the manufacture of cosmetics. Accordingly, a complex pigment purification process is used to remove the pigment from the natural product extract, but in the case of red ginseng, the existing process does not obtain a distinct effect due to manufacturing yield and economic reasons. The red ginseng extract that has not been processed separately also exhibits a dark brown color, and if this red ginseng extract is included in the product, the color of the product becomes dark and the preference is deteriorated. Patent Document 1 discloses a point of improving the chromaticity by decolorizing the color of the raw material.

The present inventors have studied a method of alleviating the chromaticity of red ginseng extract, and by using a membrane having a predetermined molecular weight fraction during ultrafiltration, the chromaticity of the red ginseng extract is reduced, and active ingredients such as ginsenoside, AFG, and arginine are strengthened. Confirmed.

Korean Patent Publication No. 951812

An object of the present invention is to provide a red ginseng extract with improved colority and enhanced active ingredient.

As an aspect for achieving the object of the present invention, an aspect of the present invention provides a method for preparing a red ginseng extract with reduced coloration comprising: ultrafiltration of a red ginseng extract.

In addition, another aspect of the present invention provides a red ginseng extract with reduced chromaticity prepared by the above manufacturing method.

In addition, another aspect of the present invention provides a cosmetic composition or a food composition comprising a red ginseng extract with reduced color.

The red ginseng extract with reduced chromaticity of the present invention exhibits a reduced chromaticity compared to the conventional red ginseng extract, but contains an active ingredient. Therefore, even when included as a raw material in cosmetics or food, the red ginseng extract does not alter the color of the product and exhibits physiological activity. It works.

1 shows the chromaticity of the permeated red ginseng extract according to the fractional molecular weight of the membrane used when ultrafiltration of the red ginseng extract.
FIG. 2 shows a lyophilized product of red ginseng extract with reduced chromaticity obtained by ultrafiltration of a red ginseng extract and a solid prepared by adding an excipient.

Hereinafter, the present invention will be described in detail.

The present invention provides a method for producing a red ginseng extract with reduced chromaticity comprising: ultrafiltration of the red ginseng extract.

The red ginseng is obtained by steaming ginseng.

The ginseng is Korean ginseng ( Panax ginseng ), Hoegi ginseng (P. quiquefolius ), Jeonchil ginseng (P. notoginseng ), jukjeolsam (P. japonicus ), trifolium ginseng (P. trifolium ), Himalayan ginseng (P. pseudoginseng ), Vietnamese ginseng. ( P. vietnamensis ), or American ginseng (P. quinquefolium ), and the like. Ginseng is generally classified into white ginseng and red ginseng according to the processing method, and white ginseng refers to unprocessed ginseng mined in the field, that is, fresh ginseng as it is dried, and red ginseng is dried and processed by steaming fresh ginseng. Several chemical changes are involved, including amino acid changes. Red ginseng produces ginsenosides that do not exist in ginseng by the heat applied during the manufacturing process, and the unique active ingredient of red ginseng has cancer prevention action, cancer cell growth inhibition action, blood pressure lowering action, brain nerve cell protection, and learning ability improvement action. Show.

The red ginseng of the present invention is a ginseng leaf, young shoot, stem, stem bark, root, root bark, seed, fruit, immature fruit, ripe fruit, flesh, pericarp, flower, stamen group (androecium), pistil group (gynoecium), Any one selected from the group consisting of calyxes, stamens, petals, calyx pieces, carpels, and combinations thereof can be obtained by steaming 9 times. The stamen group represents the entire stamen in one flower, and the pistil group represents the entire pistil in one flower. The carpel is a constituent element that makes a pistil of a flower, and represents a modified form of leaves, which is generally referred to as a flower leaf. The ginseng may be a root, but is not limited thereto.

The term'extract' as used in the present invention refers to a material extracted from a raw material by any method, and is used to include all of the extracted extract, the concentrate obtainable therefrom, the dried product and powder of the concentrate without limitation. do.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones.

When the extract is obtained by extracting from the raw material, all conventionally known extraction methods such as solvent extraction, ultrasonic extraction, filtration and reflux extraction may be used as the extraction method, and may be prepared by using a solvent extraction method or a reflux extraction method. . The extraction process may be repeated several times, and thereafter may be additionally subjected to steps such as concentration or freeze-drying. For example, the obtained extract is concentrated under reduced pressure to obtain a concentrate, and the concentrate is freeze-dried, and then a high-concentration extract powder can be prepared using a grinder. The extract also includes a fraction obtained by further fractionating the extract.

The extract may be extracted using water, an organic solvent, or a mixture thereof as an extraction solvent. The organic solvent is alcohol, a lower alcohol having 1 to 4 carbon atoms, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene, and their It may be any one selected from the group consisting of a mixed solvent, and when preparing the extract, the solvent may be any one selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. Can be water.

The manufacturing method may further include a step of diluting the red ginseng extract to 0.1 Brix to 30 Brix before ultrafiltration. The Brix adjustment of the red ginseng extract is to lower the viscosity of the red ginseng extract to make it a viscosity capable of ultrafiltration. The red ginseng extract may be 0.1 Brix to 29 Brix, 1 Brix to 29 Brix, 2 Brix to 27 Brix, 2 Brix to 24 Brix, 2 Brix to 21 Brix, 2 Brix to 18 Brix, 3 Brix to 18 Brix. When the Brix of the red ginseng extract is within the above range, it is applicable to ultrafiltration, and the fraction obtained through ultrafiltration may have a reduced chromaticity, and an active ingredient may be enhanced. If the Brix of the red ginseng extract is less than the lower limit, it can be applied to ultrafiltration, but there is a problem in that the production efficiency decreases because the yield in the process is decreased due to the large amount of liquid to be filtered. If the Brix of the red ginseng extract exceeds the upper limit, the viscosity of the red ginseng extract may be high, and thus the yield of the fraction permeating through ultrafiltration for a long time may be low.

The fractional molecular weight of the membrane used during ultrafiltration may be 10 kDa or less, 5 kDa or less, 3 kDa or less, 1 kDa or less, 600 Da or less. When the fractional molecular weight of the ultrafiltration membrane is 10 kDa or less, the fraction of the red ginseng extract that has passed through the membrane has a lower chromaticity than that of the red ginseng extract before filtration, resulting in a higher aesthetic acceptability. When using a membrane with an ultrafiltration membrane having a molecular weight of more than 10 kDa, the fraction of the red ginseng extract that has passed through this membrane contains a large amount of browning substances and maintains color similar to that of the red ginseng extract before filtration, when added to products such as cosmetics or food. Acceptability can be reduced.

The red ginseng extract with reduced color may have passed through an ultrafiltration membrane used during ultrafiltration. Fractions that have passed through the ultrafiltration membrane have a reduced chromaticity compared to the red ginseng extract before filtration, but the fractions that have not passed through the ultrafiltration membrane maintain or increase their chromaticity to a similar degree to that of the red ginseng extract before filtration.

The manufacturing method may further include the step of obtaining a freeze-dried product by adding an excipient to the red ginseng extract with reduced color and freeze-drying when the fraction prepared as a result of ultrafiltration has viscosity. The fraction produced as a result of the ultrafiltration may have passed through the ultrafiltration membrane when a membrane having a molecular weight cutoff of 5 kDa or less during ultrafiltration is used. The excipient is an inert substance, does not exert a direct medicinal effect, is a substance added to facilitate product intake or to form a certain form, and can be applied to the present invention without limitation as long as it is an excipient used in the fields of cosmetics and food. As excipients used in the manufacture of powder, starch, dextrin, maltodextrin, lactose, glucose, maltose, gelatin, gum arabic, cyclodextrin, and the like can be used, among which dextrins can be used. Dextrins represent the hydrolysis products of starch excluding glucose and maltose, and have different properties depending on the dextrose equivalent (DE) representing the degree of decomposition, but generally have low hygroscopicity, excellent dissolution and dispersibility, and improved texture.

The content of the excipient may be included in 5 parts by weight to 30 parts by weight, 5 parts by weight to 25 parts by weight, 5 parts by weight to 20 parts by weight, 5 parts by weight to 15 parts by weight based on 100 parts by weight of the red ginseng extract with reduced color. . If the excipient is included below the lower limit, the red ginseng extract with reduced color is not powdered, and thus the convenience of handling when used as a raw material for a product decreases. When the excipient is included in excess of the upper limit, the content of the red ginseng extract with reduced color may decrease, so that the physiological activity of the red ginseng extract may be lowered.

The present invention provides a red ginseng extract with reduced chromaticity prepared by the above manufacturing method.

When the red ginseng extract with reduced chromaticity is 1.27 Brix, the chromaticity may be 0.1500 or less, 0.0300 to 0.1500, 0.0350 to 0.1400, 0.0400 to 0.1300, 0.0450 to 0.1250, and 0.0500 to 0.1200 at 440 nm. If the color of the red ginseng extract is within the above range, even if it is added as a raw material to products such as cosmetics and food, it does not affect the color of the product, so the preference is increased. If the color of red ginseng extract exceeds the upper limit, it is difficult to apply to cosmetics and foods because it contains a large amount of browning substances.

The red ginseng extract with reduced chromaticity may include any one selected from the group consisting of ginsenoside, AFG, and arginine.

The ginsenoside may include any one selected from the group consisting of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1.

The sum of the contents of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1 is 0.2 mg/g or more, 0.3 mg/g or more, 0.2 mg/g to 30 mg/g , 0.2 mg/g to 25 mg/g, 0.2 mg/g to 20 mg/g, 0.2 mg/g to 15 mg/g, 0.2 mg/g to 10 mg/g, 0.3 mg/g to 10 mg/g Can be

The sum of the contents of ginsenosides Rg1, Rb1 and Rg3 is 0.1 mg/g or more, 0.1 mg/g to 12 mg/g, 0.1 mg/g to 10 mg/g, 0.1 mg/g to 8 mg/g, 0.1 It may be from mg/g to 6 mg/g and from 0.1 mg/g to 5 mg/g.

Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1 total 100 parts by weight of the total content of ginsenosides Rg1, Rb1 and Rg3 is 40 parts by weight or more, It may be 40 to 75 parts by weight, 40 to 70 parts by weight, 40 to 65 parts by weight, 40 to 60 parts by weight, 40 to 55 parts by weight.

The sum of the contents of the ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1; The sum of the contents of ginsenosides Rg1, Rb1 and Rg3; Or ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s, and the sum of the total content of the ginsenosides Rg1, Rb1 and Rg3 relative to 100 parts by weight of the total content of Rh1; In case, physiological activity by ginsenoside appears.

The content of the AFG is 10 mg/g to 40 mg/g, 10 mg/g to 37 mg/g, 12 mg/g to 34 mg/g, 14 mg/g to 31 mg/g, 16 mg/g to It may be 28 mg/g, 18 mg/g to 28 mg/g. When the content of AFG is within the above range, the physiological activity of AFG, such as treatment of obesity and diabetes, appears.

The content of arginine is 5 mg/g to 40 mg/g, 5 mg/g to 38 mg/g, 7 mg/g to 36 mg/g, 9 mg/g to 34 mg/g, 11 mg/g to It may be 32 mg/g. If the content of arginine is within the above range, since it exhibits adsorption to skin and hair, the functionality of a cosmetic including a red ginseng extract having a reduced coloration as a raw material may be enhanced.

In addition, the present invention provides a cosmetic composition comprising a red ginseng extract with reduced color.

The red ginseng extract with reduced color is 0.001% to 60% by weight, 0.01% to 50% by weight, 0.1% to 45% by weight, 1% to 40% by weight, 1% by weight, based on 100% by weight of the cosmetic composition It may be included in an amount of% to 20% by weight. If the content of the red ginseng extract with reduced color in the cosmetic composition is less than 0.001% by weight, the physiological activity by the red ginseng extract with reduced color may not be sufficiently expressed, and if it exceeds 60% by weight, there are problems in the formulation. Can occur.

The cosmetic composition of the present invention may be prepared in a liquid or solid form using bases, auxiliary agents, and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form may include, for example, lotions, creams, lotions, and bath products, but are not limited thereto. Bases, auxiliary agents and additives commonly used in the cosmetic field are not particularly limited, and examples include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

The cosmetic composition of the present invention may include not only red ginseng extract with reduced color, but also components commonly used in cosmetic compositions, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, And it may include a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, It may be formulated as an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as carrier components Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Propellants such as carbon, propane/butane or dimethyl ether may be included.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in duplicate with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having an excellent effect of increasing the amount of pro-collagen synthesis according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to a user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

In addition, the present invention provides a food composition comprising a red ginseng extract with reduced color.

The food composition of the present invention may be provided in the form of powder, granule, tablet, capsule, syrup or beverage, and the food composition may be used with other foods or food additives in addition to the red ginseng extract with reduced color of the present invention as an active ingredient. I can. The mixing amount of the active ingredient may be appropriately determined according to the purpose of its use, for example, prevention, health or therapeutic treatment.

The red ginseng extract with reduced color is in the food composition from 0.001% to 60% by weight, for example, from 0.01% to 50% by weight, from 0.1% to 45% by weight, from 1% to 40% by weight, from 1% to 20% by weight. It may be included in an amount of weight %. If the content of the red ginseng extract with reduced chromaticity in the food composition is less than 0.001% by weight, the physiological activity of the red ginseng extract with reduced chromaticity may not be sufficiently expressed, and when it exceeds 60% by weight, the problem of formulation Can occur.

There is no particular limitation on the type of food of the present invention, and examples include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea. , Drinks, alcoholic beverages, and vitamin complexes.

Hereinafter, the present invention will be described in detail with reference to Preparation Examples and Experimental Examples.

However, the following Preparation Examples and Experimental Examples are for illustrative purposes only, and the contents of the present invention are not limited by the following Preparation Examples and Experimental Examples.

Preparation Example 1. Preparation of red ginseng water extract

To 500 g of dried red ginseng ( Panax ginseng ), 6 times of distilled water was added and extracted at 85°C for 10 hours. For the residue, 6 times of distilled water was added and extraction was further performed 1 to 4 times for 10 hours, and the extract was filtered and then all mixed. A red ginseng concentrate was obtained by concentrating to 72 Brix using a rotary vacuum concentrator (BUCHI, R-220).

Preparation Example 2. Filtration of red ginseng concentrate using ultrafiltration

During ultrafiltration, the red ginseng concentrate was diluted to 16 Brix and used. An ultrafiltration membrane separation device (Ampo Model 2319 Co., Ltd.) was used. For filtration, membranes having cutoff molecular weights of 600 MW, 1000 MW, 3000 MW, 5000 MW, and 10000 MW, respectively, were used. First, a membrane having a molecular weight cutoff of 10000 MW was used for filtration, and when 10000 MW was applied, the permeated liquid was recovered, and the recovered liquid was filtered using a membrane having a molecular weight cutoff of 5000 MW. As described above, filtration was performed sequentially from the membrane having a large molecular weight cutoff to the small membrane. The liquids permeated through each of the ultrafiltration membranes used above were used as sample names. For example, a sample name of '10 kDa ~'indicates a liquid that was not permeated when using a 10000 MW membrane, and '5 to 10 kDa' indicates a liquid that was not permeated through a 10000 MW membrane and a 5000 MW membrane, and'~ The 5 kDa' fraction refers to a mixture of fractions obtained using a membrane having a molecular weight of 5 kDa or 5 kDa or less. The obtained fraction was lyophilized to facilitate product utilization, or the dried product was powdered to form a uniform shape.

Experimental Example 1. Evaluation of chromaticity of fractions

When the red ginseng concentrate was used as a raw material for cosmetics or food, it was confirmed that the chromaticity of the fractions obtained by ultrafiltration was improved because the acceptability decreased when the color was high. After diluting the fraction with the same sugar content (1.27 Brix), absorbance was measured at 440 nm to obtain the chromaticity of the diluted fraction. The results are shown below.

Before filtration ~ 600 Da 600 Da to 1 kDa 1 kDa to 3 kDa 3 kDa to 5 kDa ~ 5 kDa
(Fraction mix) 5 kDa to 10 kDa
10 kDa ~ Chromaticity 0.1584 0.0547 0.0566 0.0694 0.0742 0.0637 0.1108 0.1911

As shown in Table 1 and FIG. 1, when using a membrane with a molecular weight cutoff of 10 kDa or less, the permeated red ginseng concentrate had a chromaticity of 0.11 or less. Was found to be significantly reduced.

From the above results, it can be seen that in order to improve the chromaticity of the red ginseng concentrate, a membrane of 10 kDa or less should be used. In addition, since the components included for each raw material are different, it can be seen that when ultrafiltration is used for the purpose of color adjustment, an appropriate film should be used depending on the raw material.

Experimental Example 2. Identification of the active ingredient contained in the fraction

In Experimental Example 1, when a membrane having a molecular weight of 10 kDa was confirmed to have a chromaticity in a range applicable to cosmetics or food, the content of the active ingredient contained in the permeated fraction was confirmed. The content of the active ingredient was corrected to 72 Brix, and the results are shown below. The sum of the three ginsenosides represents the sum of the contents of ginsenosides Rg1, Rb1 and Rg3, and the sum of the 11 ginsenosides is ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, It represents the sum of the contents of Rg3r, Rg3s and Rh1. The unit of the content of ginsenoside is mg/g, the unit of the content of AFG is mg/g, and the unit of the content of arginine is mg/g.

number Item ~ 600 Da 600 Da ~
1 kDa 1 kDa ~
3 kDa 3 kDa ~
5 kDa 5 kDa to 10 kDa One Ginsenoside Rb1 - - 0.10 0.26 1.46 2 Ginsenoside Rb2 - - - - 0.40 3 Ginsenoside Rc - - - 0.09 0.66 4 Ginsenoside Rd - - - - 0.13 5 Ginsenoside Re - 0.18 1.21 1.28 1.99 6 Ginsenoside Rf - - 0.30 0.34 0.53 7 Ginsenoside Rg1 - 0.18 1.41 1.28 1.72 8 Ginsenoside Rg2s - - 0.10 0.17 0.26 9 Ginsenoside Rg3r - - - - - 10 Ginsenoside Rg3s - - - - - 11 Ginsenoside Rh1 - - 0.20 0.26 0.26 12 Ginsenoside 3 types - 0.18 1.51 1.53 3.18 13 11 kinds of ginsenosides - 0.37 3.32 3.66 7.41 14 AFG - 19.97 29.40 24.68 21.44 15 Arginine 26.08 22.90 15.10 18.13 13.90

As a result of the analysis, it was confirmed that the sum of the contents of ginsenosides Rg1, Rb1 and Rg3 was 3 mg/g or more in the fractions permeated when the membrane with a molecular weight cutoff of 10 kDa was applied, which was suitable for the content standard of the functional food code. In addition, the sum of the contents of ginsenosides Rg1, Rb1 and Rg3 was found to be 40% by weight or more compared to 100% by weight of the total content of 11 types of ginsenosides. In addition, the content of AFG and arginine was high. In particular, the content of AFG and arginine was higher than that of unfiltered red ginseng concentrate.

Through the above results, the red ginseng concentrate was separated into a membrane with a molecular weight cutoff of 10 kDa, and the permeated fraction had low chromaticity and contained active ingredients such as ginsenoside, AFG, and arginine. It can be seen that it can be used as a raw material during manufacture.

Experimental Example 3. Preparation of lyophilized powder of fraction

The fraction obtained in Preparation Example 2 was lyophilized to facilitate product utilization and powdered to form a uniform shape. The low-molecular fraction ('~5 kDa' fraction) fractionated into a viscous membrane of 5 kDa or less was uniformly mixed through dissolution after addition of dextrin, and then re-dried (lyophilized) to form a solid and then pulverized. Powdered.

As a result of powdering, the red ginseng concentrate was separated into a membrane having a molecular weight cutoff of 5 kDa or a membrane having a molecular weight of 5 kDa or less, and the'~ 5 kDa' fraction, which was the permeated fraction, was in a solid state immediately after lyophilization and was not powdered due to high viscosity. . In order to pulverize the'~ 5 kDa' fraction, dextrin was added to the'~ 5 kDa' fraction in a ratio of 9:1 by weight to powder (Fig. 2).

Red ginseng concentrate without ultrafiltration; When the red ginseng concentrate is separated by a membrane having a molecular weight cutoff of 5 kDa, it is not permeated, and a fraction of '5 kDa ~ 10 kDa', which is a fraction permeated when separated by a membrane having a molecular weight of 10 kDa; And the '10 kDa ~'fraction that was not permeated when the red ginseng concentrate was separated by a membrane having a molecular weight cutoff of 10 kDa; 100% was powdered in a freeze-drying process. Except for the '5 kDa ~ 10 kDa' fraction, the red ginseng concentrate or the '10 kDa ~'fraction still had high chromaticity (FIG. 2).

From the above results, in the case of the'~ 5 kDa' fraction and the '5 kDa ~ 10 kDa' fraction, that is, the fraction permeated using a membrane with a molecular weight of less than 10 kDa has low chromaticity and contains active ingredients, it is used as a raw material for the product. It can be seen that the possibility of use is high, and in the case of the'~ 5 kDa' fraction, it was found that the use of dextrin is essential for powdering in order to increase the convenience during product processing.

Preparation Example 3. Preparation of cosmetic composition

3-1. Preparation of essence

Essences were each prepared according to the content (parts by weight) shown in Table 3 below using the red ginseng extract with reduced color.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 Red ginseng extract with reduced color 1.5 Beeswax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Appropriate amount

3-2. Manufacturing of flexible cosmetic water

Softening lotion containing red ginseng extract with reduced color as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDTA 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated Castor Oil 1.00 ethanol 30.00 Red ginseng extract with reduced color 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

3-3. Preparation of nourishing cream

A nutritional cream containing red ginseng extract with reduced color as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitansquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 Red ginseng extract with reduced color 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl acetate Appropriate amount Triethanolamine Appropriate amount Flavoring agent Appropriate amount Purified water Balance

3-4. Lotion manufacture

A lotion containing red ginseng extract with reduced coloration as an active ingredient was prepared as shown in Table 6 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbital Sesquioleate 0.6 Squalene 4.8 Macadamia oil 2 Jojoba Oil 2 Tocopherol acetate 0.4 Methylpolysiloxane 0.2 Tocopherol acetate 0.4 1,3-butylene glycol 4 Xanthan gum 0.1 glycerin 4 d-pandenol 0.15 Red ginseng extract with reduced color 1.0 Allantoin 0.1 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Appropriate amount

Manufacture Example 4. Manufacture of food

4-1. Manufacture of flour food

0.5 to 5.0 parts by weight of the red ginseng extract with reduced color of the present invention was added to flour, and bread, cakes, cookies, crackers, and noodles were prepared using this mixture.

4-2. Preparation of soups and gravies

0.1 to 5.0 parts by weight of the red ginseng extract with reduced color of the present invention was added to soups and juices to prepare health-promoting meat products, noodle soups, and juices.

4-3. Preparation of ground beef

Ground beef for health promotion was prepared by adding 10 parts by weight of the red ginseng extract with reduced chromaticity of the present invention to ground beef.

4-4. Manufacture of dairy products

5 to 10 parts by weight of the red ginseng extract with reduced color of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

4-5. Manufacturing of wiresik

Brown rice, barley, glutinous rice, and adlay were gelatinized and dried by a known method, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

Black soybeans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, and then roasted, and then prepared into powder having a particle size of 60 mesh by a grinder.

The red ginseng extract with reduced chromaticity of the present invention was concentrated under reduced pressure in a vacuum concentrator, sprayed and dried with a hot air dryer, and the resulting dried product was pulverized with a grinder to a particle size of 60 mesh to obtain a dry powder.

The grains, seeds and red ginseng extracts of the present invention with reduced chromaticity were mixed in the following ratio to prepare them.

Cereals (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley), seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame), red ginseng extract with reduced color of the present invention (3 parts by weight) Part), Ganoderma lucidum (0.5 parts by weight), Rehmannia (0.5 parts by weight)

4-6. Manufacturing of health drinks

Subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and 5 g of red ginseng extract with reduced color of the present invention are homogeneously blended. After instant sterilization, it was prepared by packaging it in a small container such as a glass bottle or a plastic bottle.

4-7. Manufacture of vegetable juice

Vegetable juice was prepared by adding 5 g of red ginseng extract with reduced color of the present invention to 1,000 ml of tomato or carrot juice.

4-8. Manufacture of fruit juice

A fruit juice was prepared by adding 1 g of the red ginseng extract with reduced color of the present invention to 1,000 ml of apple or grape juice.

Claims (19)

Ultrafiltration of the red ginseng extract using a membrane having a molecular weight cutoff of 5 kDa or less;
Adding an excipient to the ultra-filtered fraction above; And
Powdering the fraction to which the excipient is added; a method of producing a red ginseng extract having reduced chromaticity. The method according to claim 1,
When preparing the red ginseng extract, the solvent is any one selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. The method according to claim 2,
The solvent is water, a method of producing a red ginseng extract with reduced chromaticity. The method according to claim 1,
Before ultrafiltration, the step of diluting the red ginseng extract to 0.1 Brix to 30 Brix; Method for producing a red ginseng extract with reduced chromaticity further comprising. The method according to claim 1,
The ultrafiltration step,
Fractions ultra-filtered using a membrane having a molecular weight cutoff of 600 Da or less,
Ultrafiltered fraction using a membrane having a molecular weight cutoff of more than 600 Da and less than or equal to 1 kDa,
Ultrafiltered fraction using a membrane having a molecular weight cutoff of more than 1 kDa and less than or equal to 3 kDa, and
A method for producing a red ginseng extract with reduced chromaticity comprising mixing the ultra-filtered fraction using a membrane having a molecular weight cutoff of more than 3 kDa and less than or equal to 5 kDa. The method according to claim 1,
The red ginseng extract with reduced chromaticity is a method of producing a red ginseng extract with reduced chromaticity that has passed through an ultrafiltration membrane used during ultrafiltration. The method according to claim 1,
The pulverizing step is a step of obtaining a freeze-dried product by freeze-drying the fraction to which the excipient is added; delete A red ginseng extract with reduced chromaticity prepared by the method for preparing a red ginseng extract having a reduced chromaticity of any one of claims 1 to 7. The method of claim 9,
When the red ginseng extract with reduced chromaticity is 1.27 Brix, the red ginseng extract with reduced chromaticity having an absorbance of 0.1500 or less at 440 nm. The method of claim 9,
The red ginseng extract with reduced chromaticity is a red ginseng extract with reduced chromaticity comprising any one selected from the group consisting of ginsenoside, arginine-fructose-glucose (arginine-fructose-glucose, AFG) and arginine. The method of claim 11,
The ginsenoside is a red ginseng extract with reduced chromaticity comprising any one selected from the group consisting of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1. The method of claim 12,
Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1 total content of 0.2 mg / g or more red ginseng extract with reduced color. The method of claim 12,
Ginsenosides Rg1, Rb1, and Rg3 total content is 0.1 mg / g or more red ginseng extract with reduced chromaticity. The method of claim 12,
Chromaticity in which the total content of ginsenosides Rg1, Rb1 and Rg3 is 40 parts by weight or more compared to 100 parts by weight of the total content of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2s, Rg3r, Rg3s and Rh1 Red ginseng extract with reduced. The method of claim 11,
The content of the AFG is 10 mg / g to 40 mg / g of red ginseng extract with reduced chromaticity. The method of claim 11,
The content of the arginine is 5 mg / g to 40 mg / g of red ginseng extract with reduced chromaticity. A cosmetic composition comprising a red ginseng extract having reduced chromaticity of claim 9. A food composition comprising a red ginseng extract having reduced chromaticity of claim 9.

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