KR20090057511A - Decolorizing method of white lotus extract and cosmetic composition containing the same as an active ingredient - Google Patents

Abstract

A method for decolorizing white louts extract using radioactive rays is provided to remove the color of white lotus extract and maintain the content of polyphanol of white lotus. A method for decolorizing a white lotus extract solution comprises: a step of drying and pulverizing a white lotus and dipping in solvent; and a step of irradiating to the white lotus extract solution and removing color. A solvent for extracting the white lotus is water, alcohol of C1-C4 or their mixture. The alcohol is ethanol. The radiation is selected from gamma ray, electronic beam, and X-ray and ionic beam.

Description

Decolorizing method of white lotus extract and cosmetic composition containing the same as an active ingredient}

The present invention relates to a method for decolorizing white lotus extract using radiation and a cosmetic composition containing the same as an active ingredient.

Lotus is divided into red lotus and white lotus, and is divided into lotus root, lower leaf, lotus, lotus, lotus, lotus root, and lotus root, and is a plant of the water lily family used for edible, medicinal and ornamental purposes. In particular, seeds, rhizomes and dried leaves have been used as drugs such as hemostasis, branches, blood transfusions, fish blood, antipyretic, pneumonia, nervous breakdown, low back pain, gonorrhea, nephritis and gynecological diseases. Converging action, lower lobe has been widely used for vasodilation and antibacterial action. Recently, white lotus has also been used as herbal tea, processed foods and dyestuffs.

Republic of Korea Patent No. 479659 is characterized in that the use of white lotus leaf extract as a blending water in the blending process during the manufacturing of noodles, and further comprises a steaming process for alpha nyeonseo through the white lotus leaf steam box to significantly enhance the flavor of wheat flour The present invention provides a method for producing noodles using white lotus leaf extract, which has been removed, the grease adsorbed in the milking process is remarkably lowered, eliminating feeling, showing a protein taste, and markedly decreasing the swelling of the stomach after eating.

In addition, the Republic of Korea Patent No. 395872 is a) a washing step of washing the white lotus leaf, b) the first cutting step of dividing the washed white lotus leaf into 2 to 6 equally, c) a thickening step of steaming for 5 to 30 seconds with water vapor, d ) Secondary cutting step to finely cut the white lotus leaf with a cutter, e) the first drying step to make the moisture of the white lotus leaf 60 ~ 85% by stirring in the hot wind, f) 에서 in the cooking pot of 90 ~ 160 ℃ After boiling, the process of rubbing 2 ~ 5 times using a bibimulator, and g) white lotus leaf tea consisting of a secondary drying step of drying with hot air so that the final moisture content of the white lotus leaf tea is 3-10%. It provides a method of manufacturing.

On the other hand, technical attempts to develop cosmetic materials using white lotus have not been actively made yet. Recently, a case of using gamma rays to remove the pigment of green tea extract has been reported, but these results have a problem that the removed color is reduced to the original extract color when stored at room temperature 25 ℃, to compensate for this Ascorbic acid is used as an additive to improve color (Korean Patent Publication No. 10-2001-0055493).

Although many functional cosmetics are currently used with added synthetics, there is a need for a technical approach to a method of preparing a cosmetic composition using white lotus components beneficial to the human body and a method of decolorizing extracts for using the white lotus components as suitable cosmetic ingredients. .

Thus, the inventors of the present invention, while studying a method for removing the color of the white lotus extract while maintaining the antioxidant activity of natural white lotus, found a method for decolorizing the extract by applying radiation, and completed the present invention.

It is an object of the present invention to provide a method for decolorizing white lotus extract that maintains antioxidant activity.

Another object of the present invention to provide a cosmetic composition comprising the white lotus extract extracted by the method as an active ingredient.

In order to achieve the above object, the present invention comprises the steps of drying and grinding white lotus in each part (root, stem, leaves and flowers) and then extracting the extract through a solvent; And irradiating the extract with radiation to remove the color of the white lotus extract.

In another aspect, the present invention provides a cosmetic composition comprising the white lotus extract extracted by the method as an active ingredient.

According to the present invention, the white lotus extract is easily extracted through a solvent, and the white lotus extract is irradiated with radiation to decolorize the extract so that the content of the polyphenol having antioxidant activity contained in natural white lotus is maintained in the decolorizing process. Since the color of the natural white lotus extract can be improved, it can be usefully used as an active ingredient of an antioxidant functional cosmetic composition.

In order to achieve the above object, the present invention comprises the steps of drying and grinding white lotus and then immersed in a solvent (step 1); And it provides a decolorization method of the white lotus extract comprising the step (step 2) of irradiating the white lotus extract extracted in step 1 to remove the color.

Hereinafter, the present invention will be described in detail step by step.

First, the step 1 according to the present invention is a step of extracting a white lotus extract through a solvent, specifically, grinding and drying each part of the white lotus, immersing and leaving the dried product in a solvent, and then filtering the solution and reducing the pressure. Concentration.

The site of the white lotus in the step may be used leaves, stems, flowers, roots and the like. It is preferable to use a white lotus leaf from a viewpoint that a white lotus leaf contains abundant polyphenol which has antioxidant activity, and can be used as an active ingredient of a functional cosmetic composition. Drying after grinding in the above step is preferably carried out at room temperature for 3 to 4 days.

Extraction of the dried white lotus can be carried out by immersing and standing in a solvent. Water, C ₁ ~ C ₄ alcohol or a mixed solvent thereof may be used as the extraction solvent, it is preferable to use methanol or ethanol. In order to facilitate the extraction in the above step, the immersion and standing step is preferably performed for 24 to 48 hours at room temperature. After the settling, the extract is filtered through filter paper and concentrated under reduced pressure to obtain a final extract.

Step 2 according to the present invention is a step of decolorizing the extract by irradiation with white lotus extract.

The radiation used in step 2 may include gamma rays, electron beams, X-rays, ion beams, and the like. Among these, gamma rays are preferable to use gamma rays from the viewpoint of better material permeability than other radiations. Irradiation of the extract can effectively decolorize the white lotus extract without affecting the antioxidant activity of the extract.

The radiation in step 2 is preferably irradiated so that the total absorbed dose is in the range of 10 ~ 50 kGy. If the total absorbed dose is less than 10 kGy, there is a problem that the decolorization is not completely done, there is a problem that even more than 50 kGy does not decolorize. The device for irradiation may be used without limitation as long as the device can irradiate a constant absorbed dose for a certain time.

In addition, the present invention provides a cosmetic composition having an antioxidant function, the white lotus extract obtained by the bleaching method as an active ingredient. In the case of the white lotus extract of Examples 1 to 3 according to the present invention, it can be seen that the antioxidant activity is maintained irrespective of gamma irradiation through Experimental Examples 1, 2, and 3, and the total gamma-ray total absorption dose is increased through Experimental Example 4. It can be seen that the chromaticity is improved by increasing the difference in total chromaticity. Therefore, the white lotus extract obtained through the above step can be used as an active ingredient of a cosmetic composition having an antioxidant function as an extract with improved color while maintaining the antioxidant activity of natural white lotus.

Hereinafter, the present invention will be described in detail by Examples and Experimental Examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited by the following examples.

< Example  1> Extraction process of white lotus leaf and decolorization process of the extract Ⅰ

Step 1: Extraction Process of White Lotus Root

White lotus leaf (1.3 kg) was dried at room temperature for 3 days and then ground. The grinding result was immersed in 70% ethanol (7.8 l) and left at room temperature for 24 hours. The extract was filtered through filter paper and concentrated under reduced pressure.

Step 2: Decolorization Process of Extract

Gamma rays were irradiated to remove the pigment of the extract. For gamma irradiation, a cobalt-60 gamma irradiation facility (Point source AECL, IR-79, MDS Nordion International Co. Ltd., Ottawa, ON, Canada) with a source of 100,000 Ci was used. The total absorbed dose was adjusted to 10 kGy by irradiation for 1 hour at a dose rate of 10 kGy.

< Example  2> Extraction Process of White Lotus Leaf and Decolorization Process of the Extract Ⅱ

Step 1: Extraction Process of White Lotus Leaf

The extraction process was performed in the same manner as in Step 1 of Example 1.

Step 2: Decolorization Process of Extract

The decolorization process was carried out in the same manner as in step 2 of Example 1 except that the gamma ray was irradiated for 2 hours to adjust the total absorbed dose to 20 kGy.

< Example  3> Extraction Process of White Lotus Leaf and Decolorization Process of the Extract Ⅲ

Step 1: Extraction Process of White Lotus Leaf

The extraction process was performed in the same manner as in Step 1 of Example 1.

Step 2: Decolorization Process of Extract

The decolorization process was performed in the same manner as in Step 2 of Example 1 except that the gamma ray was irradiated for 5 hours to adjust the total absorbed dose to 50 kGy.

< Comparative example  1> Extraction Process of White Lotus Root

Roots (500 g) of white lotus were crushed after drying at room temperature for 3 days. The grinding result was immersed in 70% ethanol (2.5 l) and allowed to stand at room temperature for 24 hours. The extract was filtered through filter paper and concentrated under reduced pressure.

< Comparative example  2> Extraction Process of White Lotus Leaf

The leaves of white lotus were dried and ground in the same manner as in Comparative Example 1 except for grinding.

< Comparative example  3> Extraction Process of White Lotus Stem

White stem was dried and ground in the same manner as in Comparative Example 1 except that it was ground.

< Comparative example  4> Extraction Process of White Lotus Flower

A white lotus flower was dried in the same manner as in Comparative Example 1 except that it was ground.

In order to measure the antioxidant activity and chromaticity of the extracts according to the Examples and Comparative Examples was carried out the following experiment.

< Experimental Example  1> Determination of Antioxidant Activity of White Lotus Extract

The white lotus extract for each part obtained in Examples 1 to 3 and Comparative Examples 1 to 4 is a free radical of 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl Antioxidant activity assay (Nature, 181, 1199-1200, 1958) using (DPPH) was modified to investigate antioxidant activity. Methanol containing root (125 μg / ml), leaf (62.5 μg / ml), stem (31.5 μg / ml), extract of flowers (15.6 μg / ml), and gamma-irradiated leaf (7.8 μg / ml) extract 150 μl of the solution was placed on a 96-well microplate, and 150 μl of DPPH (0.1 mM) solution was added to the plate, and reacted at room temperature for 10 to 15 minutes, and the absorbance was measured at 595 nm. Antioxidant activity was expressed as a 50% inhibition rate (IC ₅₀ ), and the results are shown in Table 1 below.

Gamma-irradiation (kGy) Antioxidant Activity IC ₅₀ (µg / mL) Example 1 10 15.8 Example 2 20 14.5 Example 3 50 16.1 Comparative Example 1 - 136.2 Comparative Example 2 - 15.1 Comparative Example 3 - 18.3 Comparative Example 4 - 129.2

 As shown in Comparative Example 1 to Comparative Example 4 in Table 1, the antioxidant activity of each part of the white lotus extract was the strongest in the leaves as 15.1 ㎍ / ㎖, by the change in the gamma irradiation dose to the extract Antioxidant activity of the leaf extract was changed in the range of 14.5 ㎍ / ㎖ ~ 16.1 ㎍ / ㎖ there was no significant difference. Through this, it can be seen that the white lotus leaf is most preferable as an active ingredient of the functional cosmetic composition having antioxidant activity, and gamma-ray irradiation of the white lotus leaf extract does not affect the antioxidant activity of the white lotus leaf extract.

< Experimental Example  2> Ultra-light Emitting Analyzer ( Ultra weak luminescence analyzer Superoxide using superoxide ) And Hydroxyl ( hydroxyl ) Radical  Scavenging activity measurement

For superoxide radical measurement, 1 ml of lucigenin (2 mM), 1 ml of phosphate buffered saline (pH 7.4), arginine in a quartz round-bottom flask (1 M) 28 μl, methylglyoxal (1.4 μM) 24 μl were mixed in order, for hydroxyl radical measurement, 1 mL of IBG (3 μM), 0.1 mL of FeSO ₄ (1 mM), 1.6 ml of H ₂ O ₂ (3%) and 0.05 ml of EDTA (10 mM) were added and mixed. Thereafter, the quartz round-bottom flask was placed in a black-box unit of an ultraweak chemiluminescence analyzer (BJL, Jye Horn Co. Taipei, Taiwan) to measure ultra-fine photons. When the photons were stabilized, 2 μl of the extract (1 mg / ml) of Examples 1 to 3 and Comparative Examples 1 to 4 were added to the reaction mixture, and again re-stabilized after a sudden drop in the stabilized count. The step of adding μl was repeated. After drawing the concentration-inhibition rate graph, the concentration value (IC ₅₀ ) showing the 50% inhibition rate was calculated to determine the radical scavenging effect on the extracts. The calibration for this measurement was carried out using a ¹⁴ C-light source yielding an amount of 1000 photon count / s in the voltage range of 860-886 V and performed at 6000 V for 10 minutes. The results of the measurements are shown in Table 2 below.

Gamma-irradiation (kGy) Superoxide radical scavenging IC ₅₀ (㎍ / ㎖) Hydroxyl radical scavenging activity IC ₅₀ (μg / ml) Example 1 10 0.9 0.7 Example 2 20 0.9 0.7 Example 3 50 1.0 0.7 Comparative Example 1 - 4.2 3.1 Comparative Example 2 - 1.1 0.7 Comparative Example 3 - 3.2 2.4 Comparative Example 4 - 1.7 1.3

As shown in Comparative Example 1 to Comparative Example 4 in Table 2, IC ₅₀ of the extract of Comparative Example 2 Values were 1.1 μg / ml (superoxide radical scavenging activity) and 0.7 μg / ml (hydroxyl radical scavenging activity), showing the strongest radical scavenging activity in the extracts of the leaves of the white lotus extract. Through this, it can be seen that the antioxidant activity of the white lotus leaf extract is the strongest. In addition, the radical scavenging ability of the white lotus leaf extract by the change of gamma-irradiation dose was IC ₅₀ Values of 0.9-1.1 μg / mL (superoxide radical scavenging activity) and 0.7 μg / mL (hydroxyl radical scavenging activity) did not show a big difference, and the radical scavenging activity of white lotus leaf extract was not lowered by gamma irradiation. It can be seen.

< Experimental Example  3> Polyphenols of White Lotus Extract ( polyphenol Content measurement

The total polyphenol content of the white lotus extract obtained in Examples 1 to 3 and Comparative Examples 1 to 4 is known method (John Wiley & Sons, New York, units I .1.1.1-I .1.1.8 ) Was measured by correction. After dissolving the extract in 80% ethanol, 0.1 ml of the solution was placed in a tester tube, and 0.2 ml of Folin-Ciocalteu's phenol reagent (Folin-cio: distilled water = 1: 1) was added to the tester tube. It was left to stand for about 1 minute. 3 ml of 5% sodium carbonate (Na ₂ CO ₃ ) was added to the tester tube, followed by stirring at room temperature for about 2 hours. Thereafter, the absorbance was measured at 762 nm using a Microplate reader (BioRad). The total polyphenol content for calibration was determined using a gallic acid calibration curve. The results of the measurements are shown in Table 3 below.

Gamma-irradiation (kGy) Total Polyphenol Content (mg / g) Example 1 10 232.1 Example 2 20 224.9 Example 3 50 210.1 Comparative Example 1 - 43.2 Comparative Example 2 - 213.5 Comparative Example 3 - 182.7 Comparative Example 4 - 49.6

As shown in Comparative Example 1 to Comparative Example 4 in Table 3, the white lotus leaf extract contains the most polyphenol having an antioxidant function (213.5 mg / g), Examples 1 to 3 and comparison In relation to Example 2, it can be seen that the total polyphenol content varies in the range of 210.1 mg / g to 232.1 mg / g depending on the irradiation dose of gamma rays, so that gamma irradiation does not significantly affect the polyphenol content. Through this, the white lotus leaf is most preferable to use as an active ingredient of the functional cosmetic composition having an antioxidant activity, it can be seen that the white lotus leaf extract with improved color for use as an active ingredient of the cosmetic composition through gamma irradiation Can be.

< Experimental Example  4> Measurement of chromaticity of white lotus leaf extract irradiated with gamma ray

A color difference meter (spectrophotometer, Minolta Co., Osaka, Japan) was used to measure the change in chromaticity of Examples 1 to 3 and Comparative Example 2 through lightness, redness, and yellowness. The results of the measurements are shown in Table 4 below.

Irradiation dose (kGy) brightness Redness Yellow road Difference in total chromaticity Example 1 10 16.85 -0.04 1.02 10.65 Example 2 20 19.28 0.04 0.14 11.74 Example 3 50 20.45 -0.23 -1.27 13.24 Comparative Example 2 0 17.12 3.38 11.15 -

As shown in the table, the brightness of the white lotus leaf extract was 19.28 and 20.45 in the gamma ray 20 kGy and 50 kGy irradiation groups (Examples 2 and 3), respectively, compared with 17.12 of the non-irradiation group (Comparative Example 2). It can be seen that the improvement. The redness is shown as 3.38 in Comparative Example 2, whereas in Examples 2 and 3 it is 0.04 and -0.23, respectively, it can be seen that the redness is significantly reduced. The yellowness of the white lotus leaf extract was also shown to be 11.15 in Comparative Example 2, while in Example 2 and Example 3, respectively, 0.14 and -1.27, respectively, indicating that the yellowness was significantly reduced. Through this, it can be seen that gamma irradiation improves the chromaticity of the white lotus leaf extract, it can be seen that gamma irradiation can be usefully used as a decolorization method of the extract for using the white lotus leaf as an active ingredient of the cosmetic composition.

1 a shows the chromaticity of the white lotus leaf extract not irradiated with gamma rays, b indicates the chromaticity of the extract irradiated with gamma rays of 10 kGy, c represents the chromaticity of the extract irradiated with gamma rays of 20 kGy, d It is a photograph showing the chromaticity of the extract irradiated with gamma rays of 50 kGy.

Claims (9)

Drying and grinding white lotus, followed by immersion in a solvent (step 1); And decolorizing the white lotus extract by irradiating the white lotus extract extracted in step 1 to remove color (step 2). The method for decolorizing a white lotus extract according to claim 1, wherein the white lotus is dipped in a solvent by drying and grinding one or a mixture thereof selected from the leaves, stems, flowers, and roots of the white lotus. 3. The method for decolorizing white lotus extract according to claim 2, wherein the white lotus is a leaf. The method of claim 1, wherein the extraction solvent of the white lotus extract is water, C ₁ ~ C ₄ alcohol or a mixed solvent thereof Decolorizing method of the white lotus extract, characterized in that. 5. The method for decolorizing white lotus extract according to claim 4, wherein the alcohol is ethanol. The method of claim 1, wherein the radiation is selected from the group consisting of gamma rays, electron beams, X-rays and ion beams. The decolorization method of white lotus extract according to claim 1, wherein the radiation is irradiated to the extract for 1 to 5 hours. The method for decolorizing white lotus extract according to claim 1, wherein the total absorbed dose of radiation is 10-50 kGy. Antioxidant active cosmetic composition with improved color using the white lotus extract obtained by the decolorizing method of any one of claims 1 to 8.

Images