CN103932181B - A kind ofly to release the pressure and the functional food of Improving memory and two-step fermentation preparation method thereof - Google Patents
A kind ofly to release the pressure and the functional food of Improving memory and two-step fermentation preparation method thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of releasing the pressure and the functional food of Improving memory and two-step fermentation preparation method thereof, the method comprises: asparagus solid fermentation: with the natural constituents containing Improving memory for property of medicine culture matrix, and inoculation flammulina velutipes liquid strains carries out the medicinal fungal substance that the preparation of two-way solid fermentation has Improving memory effect; Lactic acid bacteria liquid deep layer fermenting: take medicinal fungal substance as raw material, add dusty yeast after pulverizing, sodium glutamate, water makes fermentation medium, inoculating lactic acid bacterium carries out liquid deep layer fermenting and produces the GABA of releasing the pressure, and preparation has the zymotic fluid of releasing the pressure with Improving memory effect simultaneously; Adopt zymotic fluid, be prepared into releasing the pressure and the functional food of Improving memory of different dosage form.Functional food prepared by the present invention has simultaneously releases the pressure and Improving memory power function, wide market, and this preparation method stablizes, and is applicable to large-scale production.
Description
Technical field
The present invention relates to functional food and preparation field thereof, be specifically related to a kind of release the pressure and Improving memory functional food and adopt asparagus, lactic acid bacteria solid-liquid two-step fermentation to prepare the method for this functional food.
Background technology
Along with the continuous quickening of modern life rhythm, people are in life, study, competition in work is more and more fierce, stress is large, the inferior health problems such as failure of memory become puzzlement and comprise student, white collar is in the major hidden danger of interior mental worker, therefore increasing people starts to pay close attention to " brain is healthy ", release the pressure, food and the health food of Improving memory are more and more favored, the current health food for brain health is with develop immunitypty, antifatigue, Improving memory is main, and be mostly the product of simple function, there is no the health food can simultaneously released the pressure with Improving memory power.
Asparagus is described as " increasing intelligence mushroom ", and research shows that flammulina velutipes extract has effect of Improving memory.At present mostly asparagus health food with asparagus fructification for prepared by raw material, as patent 201310071534.9 " a kind of health food with eyesight-improving intelligence-developing effect " gold needle mushroom extract, Europe blueberry extract, ginkgo biloba p.e, the compatibilities such as soybean lecithin form, patent 201010622493.4 " a kind of composition for alleviating asthenopia and auxiliary improvement of memory " is by whortle extract, gold needle mushroom extract, the compatibilities such as taurine form, but use asparagus fructification high for there is cost of material, the problems such as unstable product quality, be that raw material then can avoid these problems with golden mushroom mycelium, as patent 93110333.9 " " Jinzhengu " edible fungus beverage and preparation method thereof " mainly uses golden mushroom mycelium formulated.But golden mushroom mycelium is all with conventional solid or liquid fermentation preparation, there is no the property of medicine matrix that utilizes asparagus and there is certain effect by the standby report of two-way fermentation.
Zhuan Yi etc. propose the concept of two-way fermentation first, point out that edible and medicinal fungi carries out fermentation with the Chinese medicine containing active component and has amphicheirality, the physiologic effect of 1+1>2 can be produced, as patent 201110271923.7 " a kind of fungi medicinal fungal substance with effect of lowering blood sugar and preparation method thereof " selects the property of medicine matrix with effect of lowering blood sugar and fungi strain to obtain to have as two-way fermentation and significantly reduce the medicinal fungal substance that blood sugar does not increase again the effect of insulin concentration, patent 200910058889.8 " the Zymocyte mixture containing glossy ganoderma, preparation method and application thereof " carry out two-way fermentation with glossy ganoderma and the property of medicine matrix containing the red sage root, obtain the mycoplasma fermentation end products having red rooted salvia and lucid ganoderma fungus effect concurrently.Select asparagus and there is the property of medicine matrix of Improving memory effect and carry out solid fermentation, by property of medicine matrix, the impact of golden mushroom mycelium growth metabolism process and asparagus fungal enzyme system are transformed after interior amphicheirality's solid fermentation property of medicine matrix, obtain the medicinal fungal substance having property of medicine matrix and asparagus Improving memory effect concurrently, can be used as healthy food material, the effect of 1+1>2 can be produced failure of memory person.
GABA is a kind of important inhibitory neurotransmitter, modern medicine study proves that GABA has effect that is calm neural, that release the pressure, and GABA prepared by the Ministry of Public Health's No. 12 bulletin approval fermented by lactic acid bacteria in 2009 is new resource food.The lactic acid bacteria security of screening from traditional fermented food is high, can be used for the production of GABA, as patent 200780006225.6 " can produce the lactic acid bacteria of GABA " is separated to the renin Bacillus acidi lactici can producing GABA (GABA) from unpurified soy sauce, patent 200910183478.1 " a strain Efficient Conversion Pidolidone is the seed selection of GABA lactic acid bacteria " filters out a strain and Pidolidone can be utilized to produce the Lactobacillus plantarum of GABA from self-control sauerkraut.But lactic acid bacteria can only utilize the Small molecular such as monose, disaccharides carbon source, directly can not utilize starch-containing and cellulosic natural substrates, and fungi has degradable starch and cellulosic enzyme system, starchiness, cellulosic can be decomposed into the Small molecular carbon source that can utilize for lactic acid bacteria, fungi fermentation and lactobacillus-fermented be coupled and carry out step fermentation and can address this problem.
Summary of the invention
Release the pressure and the functional food of Improving memory power for there is no at present, the invention provides a kind of release the pressure and Improving memory functional food and utilize asparagus, method that lactic acid bacteria solid-liquid two-step fermentation prepares this functional food.
Release the pressure and the two-step fermentation preparation method of functional food of Improving memory, comprise the steps:
(1) asparagus solid fermentation: with the natural constituents containing Improving memory effect for property of medicine culture matrix, inoculation flammulina velutipes liquid strains carries out the medicinal fungal substance that the preparation of two-way solid fermentation has Improving memory effect;
(2) lactic acid bacteria liquid deep layer fermenting: with the medicinal fungal substance of step (1) for raw material, add dusty yeast after pulverizing, sodium glutamate, water makes fermentation medium, inoculating lactic acid bacterium carries out liquid deep layer fermenting and produces the GABA of releasing the pressure, and preparation has the zymotic fluid of releasing the pressure with Improving memory effect simultaneously;
(3) adopt the zymotic fluid of step (2), be prepared into releasing the pressure and the functional food of Improving memory of different dosage form.
The first step of the present invention is using the natural constituents containing Improving memory effect as property of medicine culture matrix, inoculation asparagus carries out two-way solid fermentation, by property of medicine culture matrix, the impact of golden mushroom mycelium growth metabolism process and asparagus fungal enzyme system are transformed after interior amphicheirality's solid fermentation property of medicine matrix, obtain the medicinal fungal substance having property of medicine matrix and asparagus Improving memory effect concurrently; Second step is to contain the medicinal fungal substance of the nutriments such as Small molecular carbon source for raw material after asparagus degraded, add dusty yeast and sodium glutamate making fermentation medium, the lactic acid bacteria that inoculation can produce GABA is fermented, and preparation has the zymotic fluid of releasing the pressure with Improving memory effect; Finally, zymotic fluid through filtering, the technique such as concentrated, preparation prepares health food.
In step (1), described asparagus can adopt prior art, and flammulina velutipes liquid strains adopts existing common process to obtain, as specifically asparagus ACCC50424 can be selected, i.e. and the bacterial classification of Chinese agriculture Microbiological Culture Collection administrative center (ACCC) preservation.
In step (1), the inoculum concentration of described flammulina velutipes liquid strains is 2%-10%, the 2%-10%(mL/g that the volume (mL) added of namely described flammulina velutipes liquid strains is the quality (g) of property of medicine culture matrix), with 100g property of medicine culture matrix for benchmark, add the flammulina velutipes liquid strains of 2-10mL.
In step (1), the preparation of described property of medicine culture matrix: the raw material first taking following weight percentage: wholegrain barley 70%-80%, soybean 10%-20%, walnut kernel 1%-3%, fructus alpiniae oxyphyllae 1%-3%, ginkgo leaf 1%-3%, land plaster 0.5%-1.5%, by each component mixing, pulverize, then adding water to moisture weight content is 60%-65%, 116 DEG C-126 DEG C sterilizing 0.5h-1.5h.Preferably, the preparation of described property of medicine culture matrix: the raw material first taking following weight percentage: wholegrain barley 75%, soybean 15%, walnut kernel 3%, fructus alpiniae oxyphyllae 3%, ginkgo leaf 3%, land plaster 1%, by each component mixing, pulverize, then adding water to moisture weight content is 60%-65%, 121 DEG C of sterilizing 1h.
In step (1), the condition of described two-way solid fermentation is: fermentation temperature is at 20-25 DEG C, and fermentation time, at 20-30 days, is advisable with this asparagus solid fermentation condition.
In step (2), described lactic acid bacteria is plant lactobacillus, the Lactobacillus plantarum LW106(deposit number preferably screened from Pickles, Sichuan Style is: CGMCC NO.5655), namely adopt the bacterial classification of China General Microbiological culture presevation administrative center CGMCC preservation, plant lactobacillus liquid spawn adopts MRS culture medium 28 DEG C of quiescent culture 48h to obtain.
In step (2), described fermentation medium adopts the raw material of following weight portion: water 1000 weight portion, medicinal fungal substance 40-50 weight portion, dusty yeast 5-10 weight portion, sodium glutamate 15-20 weight portion, by water-soluble to medicinal fungal substance, dusty yeast and sodium glutamate, interpolation acetic acid and sodium acetate are mixed with the buffer solution that pH is 5-6,116 DEG C-126 DEG C sterilizing 20min-40min.As preferably, described fermentation medium adopts the raw material of following weight portion: water 1000 weight portion, medicinal fungal substance 50 weight portion, dusty yeast 7.5 weight portion, sodium glutamate 20 weight portion, by water-soluble to medicinal fungal substance, dusty yeast and sodium glutamate, interpolation acetic acid and sodium acetate are mixed with the buffer solution that pH is 5.5,121 DEG C of sterilizing 30min.
In step (2), the inoculum concentration of described lactic acid bacteria is 1%-5%, and the volume added of namely described lactic acid bacteria is the 1%-5% of fermentation medium volume.
In step (2), the condition of described liquid deep layer fermenting is: fermentation temperature is at 28-32 DEG C, and fermentation time, at 48-72h, is advisable with this fermentation condition.
In step (2), in described zymotic fluid, the content of GABA is 9-12g/L.
In step (3), described zymotic fluid by filtering, the technique such as concentrated, preparation is prepared into the functional food of different dosage form.
Described to release the pressure and the formulation of functional food of Improving memory power is peroral dosage form, be preferably tablet or capsule, fermentation liquor is filtered, concentrated, spraying dry, granulation, obtain tablet after compressing tablet, and fermentation liquor is filtered, concentrated, spraying dry, granulation, obtain capsule after dress capsule.Particularly; first filter, concentrate, be spray dried to powder; dried particles is obtained through boiling granulating after powder sieves; particle particle pelletizing machine carries out whole grain with 20 mesh sieves; whole grain gained particle adds weight 0.5% disintegrator carboxymethylstarch sodium relative to whole grain gained particle and weight 0.1% magnesium stearate lubricant relative to whole grain gained particle fully mixes 5 minutes encapsulating capsules or is pressed into tablet, every capsules/tablet 0.35g in multi-dimensional movement mixer.
By the functional food of releasing the pressure of preparing of above-mentioned two-step fermentation preparation method and Improving memory power, this functional food has simultaneously releases the pressure and the function of Improving memory power.
The two-way fermentation of property of medicine culture matrix of asparagus and Improving memory can prepare the medicinal fungal substance of Improving memory Be very effective, and property of medicine culture matrix is by the degradation of asparagus, medicinal fungal substance can be lactobacillus-fermented product GABA and provides nutrition and carbon source, simultaneously by degraded and the conversion of lactic acid bacteria, medicinal fungal substance more easily absorbs and utilizes.Therefore asparagus and lactic acid bacteria two-step fermentation is adopted can to prepare the functional food can simultaneously released the pressure with Improving memory power.
Compared with prior art, the present invention has following beneficial effect:
(1) the present invention has Improving memory effect according to asparagus, GABA has effect of releasing the pressure, the two compatibility has releases the pressure and Improving memory effect, provide a kind of with the preparation method of functional food of function intensified asparagus medicinal fungal substance and GABA compatibility, the method organically combines edible fungus fermented with lactobacillus-fermented, two-step fermenting is adopted to prepare the functional food can simultaneously released the pressure with Improving memory, wherein the first step utilizes asparagus to have the medicinal fungal substance of asparagus and property of medicine matrix Improving memory effect concurrently with the two-way solid fermentation preparation possessing Improving memory effect property of medicine matrix, this process can not only produce the physiological function of 1+1 > 2, also the large molecule carbon source in property of medicine matrix can be degraded to the Small molecular carbon source that lactic acid bacteria is easy to utilize, necessary nutrition and carbon source is provided for second step lactic acid bacteria liquid deep layer fermenting produces the GABA possessing the effect of releasing the pressure, simultaneously by degraded and the conversion of lactic acid bacteria, medicinal fungal substance more easily absorbs and utilizes.
(2) functional food that prepared by the present invention the experiment proved that to have and releases the pressure and Improving memory function.
(3) production method of the present invention's employing, not only cost of material is low, and whole production process all adopts cold fermentation, mild condition, does not have the absorption of harmful toxic matter, and Product Safety, added value are high, can accomplish scale production, wide market.
(4) functional food that prepared by the present invention adopts the formulation such as tablet, capsule, is convenient for carrying and takes.
Accompanying drawing explanation
Fig. 1 is that the present invention releases the pressure and the flow chart of two-step fermentation preparation method of functional food of Improving memory;
Fig. 2 is the rate of change (starting point 100%) of subjects saliva's cortisol in test example 2.
Detailed description of the invention
According to Fig. 1 technological process, the present invention is described further below, but protection scope of the present invention is not limited in this.When being below not particularly illustrated, percentage all refers to percetage by weight.
Embodiment 1
1, asparagus solid fermentation:
1. the making of property of medicine culture matrix
Formula: wholegrain barley 80%, soybean 16.5%, walnut kernel 1%, fructus alpiniae oxyphyllae 1%, ginkgo leaf 1%, land plaster 0.5%;
Compound method: by formula rate, each component mixed, pulverize, adding water to moisture weight content is 65%, 121 DEG C of sterilizing 1h, obtains property of medicine culture matrix;
2. inoculation fermentation
The liquid spawn (common process preparation) of asparagus ACCC50424 is inoculated into 1. property of traditional Chinese culture matrix by 3% volume mass ratio, the i.e. volume (mL) added of the asparagus ACCC50424 liquid spawn 3%(mL/g that is the quality (g) of property of medicine culture matrix), cultivate the medicinal fungal substance that preparation in 30 days has Improving memory effect for 20 DEG C;
2, lactic acid bacteria liquid deep layer fermenting:
1. fermented and cultured matrix manufacturing
Formula: medicinal fungal substance 40g, dusty yeast 5g, sodium glutamate 15g, running water 1L(and 1000g);
Compound method: by formula rate by water-soluble to medicinal fungal substance, dusty yeast, sodium glutamate, adds acetic acid and sodium acetate is mixed with the buffer solution that pH is 5.5, and 121 DEG C of sterilizing 30min, obtain fermentation medium;
2. by the Lactobacillus plantarum LW106(deposit number screened from Pickles, Sichuan Style be: CGMCCNO.5655) liquid spawn (MRS culture medium 28 DEG C of quiescent culture 48h) is inoculated into fermentation medium 1. in 1% ratio, namely the volume added of Lactobacillus plantarum is that 1%, 28 DEG C of quiescent culture 72h preparation of fermentation medium volume has and releases the pressure and the zymotic fluid of Improving memory effect;
3, first the zymotic fluid that prepared by the 2nd step filters, concentrates, is spray dried to powder; dried particles is obtained through boiling granulating after powder sieves; particle particle pelletizing machine carries out whole grain with 20 mesh sieves; whole grain gained particle adds 0.5% disintegrator carboxymethylstarch sodium relative to whole grain gained particle weight and 0.1% magnesium stearate lubricant relative to whole grain gained particle weight fully mixes 5 minutes in multi-dimensional movement mixer; be pressed into tablet, every sheet 0.35g.
Embodiment 2
1, asparagus solid fermentation:
1. the making of property of medicine culture matrix
Formula: wholegrain barley 80%, soybean 16.5%, walnut kernel 1%, fructus alpiniae oxyphyllae 1%, ginkgo leaf 1%, land plaster 0.5%;
Compound method: by formula rate, each component mixed, pulverize, adding water to moisture weight content is 60%; 121 DEG C of sterilizing 1h, obtain property of medicine culture matrix;
2. inoculation fermentation
The liquid spawn (common process preparation) of asparagus ACCC50424 is inoculated into 1. property of traditional Chinese culture matrix by 3% volume mass ratio, the i.e. volume (mL) added of the asparagus ACCC50424 liquid spawn 3%(mL/g that is the quality (g) of property of medicine culture matrix), cultivate the medicinal fungal substance that preparation in 30 days has Improving memory effect for 20 DEG C;
2, lactic acid bacteria liquid deep layer fermenting:
1. fermented and cultured matrix manufacturing
Formula: medicinal fungal substance 40g, dusty yeast 5g, sodium glutamate 15g, running water 1L(and 1000g);
Compound method: by formula rate by water-soluble to medicinal fungal substance, dusty yeast, sodium glutamate, adds acetic acid and sodium acetate is mixed with the buffer solution that pH is 5.5, and 121 DEG C of sterilizing 30min, obtain fermentation medium;
2. by the Lactobacillus plantarum LW106(deposit number screened from Pickles, Sichuan Style be: CGMCCNO.5655) liquid spawn (MRS culture medium 28 DEG C of quiescent culture 48h) is inoculated into fermentation medium 1. in 1% ratio, namely the volume added of Lactobacillus plantarum is that 1%, 28 DEG C of quiescent culture 72h preparation of fermentation medium volume has and releases the pressure and the zymotic fluid of Improving memory effect;
3, first the zymotic fluid that prepared by the 2nd step filters, concentrates, is spray dried to powder; dried particles is obtained through boiling granulating after powder sieves; particle particle pelletizing machine carries out whole grain with 20 mesh sieves; whole grain gained particle adds 0.5% disintegrator carboxymethylstarch sodium relative to whole grain gained particle weight and 0.1% magnesium stearate lubricant relative to whole grain gained particle weight fully mixes 5 minutes in multi-dimensional movement mixer; with capsule filling machine encapsulating capsule, every 0.35g.
Embodiment 3
1, asparagus solid fermentation:
1. the making of property of medicine culture matrix
Formula: wholegrain barley 75%, soybean 15%, walnut kernel 3%, fructus alpiniae oxyphyllae 3%, ginkgo leaf 3%, land plaster 1%;
Compound method: by formula rate, each component mixed, pulverize, adding water to moisture weight content is 63%; 121 DEG C of sterilizing 1h, obtain property of medicine culture matrix;
2. inoculation fermentation
The liquid spawn (common process preparation) of asparagus ACCC50424 is inoculated into 1. property of traditional Chinese culture matrix by 5% volume mass ratio, the i.e. volume (mL) added of the asparagus ACCC50424 liquid spawn 5%(mL/g that is the quality (g) of property of medicine culture matrix), cultivate the medicinal fungal substance that preparation in 20 days has Improving memory effect for 25 DEG C;
2, lactic acid bacteria liquid deep layer fermenting:
1. fermented and cultured matrix manufacturing
Formula: medicinal fungal substance 50g, dusty yeast 7.5g, sodium glutamate 20g, running water 1L(and 1000g);
Compound method: by formula rate by water-soluble to medicinal fungal substance, dusty yeast, sodium glutamate, adds acetic acid and sodium acetate is mixed with the buffer solution that pH is 5.5, and 121 DEG C of sterilizing 30min, obtain fermentation medium;
2. by the Lactobacillus plantarum LW106(deposit number screened from Pickles, Sichuan Style be: CGMCCNO.5655) liquid spawn (MRS culture medium 28 DEG C of quiescent culture 48h) is inoculated into fermentation medium 1. in 1% ratio, namely the volume added of Lactobacillus plantarum is that 1%, 32 DEG C of quiescent culture 48h preparation of fermentation medium volume has and releases the pressure and the zymotic fluid of Improving memory effect;
3, first the zymotic fluid that prepared by the 2nd step filters, concentrates, is spray dried to powder; dried particles is obtained through boiling granulating after powder sieves; particle particle pelletizing machine carries out whole grain with 20 mesh sieves; whole grain gained particle adds 0.5% disintegrator carboxymethylstarch sodium relative to whole grain gained particle weight and 0.1% magnesium stearate lubricant relative to whole grain gained particle weight fully mixes 5 minutes in multi-dimensional movement mixer; be pressed into tablet, every sheet 0.35g.
Embodiment 4
1, asparagus solid fermentation:
1. the making of property of medicine culture matrix
Formula: wholegrain barley 75%, soybean 15%, walnut kernel 3%, fructus alpiniae oxyphyllae 3%, ginkgo leaf 3%, land plaster 1%;
Compound method: by formula rate, each component mixed, pulverize, adding water to moisture weight content is 62%, 121 DEG C of sterilizing 1h, obtains property of medicine culture matrix;
2. inoculation fermentation
The liquid spawn (common process preparation) of asparagus ACCC50424 is inoculated into 1. property of traditional Chinese culture matrix by 5% volume mass ratio, the i.e. volume (mL) added of the asparagus ACCC50424 liquid spawn 5%(mL/g that is the quality (g) of property of medicine culture matrix), cultivate the medicinal fungal substance that preparation in 20 days has Improving memory effect for 25 DEG C;
2, lactic acid bacteria liquid deep layer fermenting:
1. fermented and cultured matrix manufacturing
Formula: medicinal fungal substance 50g, dusty yeast 7.5g, sodium glutamate 20g, running water 1L(and 1000g);
Compound method: by formula rate by water-soluble to medicinal fungal substance, dusty yeast, sodium glutamate, adds acetic acid and sodium acetate is mixed with the buffer solution that pH is 5.5, and 121 DEG C of sterilizing 30min, obtain fermentation medium;
2. by the Lactobacillus plantarum LW106(deposit number screened from Pickles, Sichuan Style be: CGMCCNO.5655) liquid spawn (MRS culture medium 28 DEG C of quiescent culture 48h) is inoculated into fermentation medium 1. in 1% ratio, namely the volume added of Lactobacillus plantarum is that 1%, 32 DEG C of quiescent culture 48h preparation of fermentation medium volume has and releases the pressure and the zymotic fluid of Improving memory effect;
3, first the zymotic fluid that prepared by the 2nd step filters, concentrates, is spray dried to powder; dried particles is obtained through boiling granulating after powder sieves; particle particle pelletizing machine carries out whole grain with 20 mesh sieves; whole grain gained particle adds 0.5% disintegrator carboxymethylstarch sodium relative to whole grain gained particle weight and 0.1% magnesium stearate lubricant relative to whole grain gained particle weight fully mixes 5 minutes in multi-dimensional movement mixer; with capsule filling machine encapsulating capsule, every 0.35g.
The present invention is further illustrated below by way of toxicity and function test.Due to capsule of the present invention and tablet place formulation, auxiliary material different outside, both primary raw material, daily measure, functional component is all identical, do not affect safety and the function of product.Therefore carry out toxicity and function test for capsule below.
Test example 1
Toxicological test:
Test objective: whether inspection product has toxicity
Test material: the capsule that embodiment 4 is obtained.
Sample: human oral's recommended intake is every day twice, each two, 0.35g every (body weight is by 60kg), amounts to dosage 0.03g/kgbw, gets capsule 's content and tests.
Animal used as test: cleaning grade ICR mouse, SD rat and feed.
Rearing conditions: temperature 20-25 DEG C, the barrier system of relative humidity 40%-70%.
Test method:
Chmice acute Oral toxicity is tested: the capsule 's content that Example 4 is obtained, is mixed with 0.500g/mL with sterilized water, and by Secondary gavage provisions in maximum gavage amount 20mL/kgbw mono-day, it is 20.0g/kgbw that tested material gives dosage.
Salmonella reversion test: the capsule 's content that Example 4 is obtained, 0.500g/mL is mixed with sterilized water, for the dosage of 5000ug/ ware, 5 dosage are established in test, 1000,200,40,8ug/ ware 4 dosage sterilized waters are mixed with 10 by certain multiple proportions stepwise dilution, 2,0.4,0.08mg/mL, application of sample amount is 0.1mL/ ware, adds 0.1mL/ ware, establish the positive, feminine gender and solvent control simultaneously, count every ware and return change clump count.
Mouse marrow cell micro nuclear test: with the endoxan of 40mg/kgbw dosage for positive control, sterilized water is negative control, test group 3 dosage are respectively 10.0g/kgbw, 5.0g/kgbw, 2.5g/kgbw, the capsule 's content sterilized water that Example 4 obtains is mixed with 0.5, 0.25, 0.125g/mL, with 20mL/kgbw gavage 2 times, adopt 30h to tested material method, give sample interval 24h twice, second time puts to death animal after giving sample 6h, get the conventional film-making of breastbone, microscopy, every mouse counts 1000 Polychromatic erythrocyte, observe the polychromatic erythrocyte containing micronucleus and observe microkernel incidence.
Mouse inbred strain: with the endoxan of 40mg/kgbw dosage for positive control, sterilized water is negative control, test group 3 dosage are respectively 10.0g/kgbw, 5.0g/kgbw, 2.5g/kgbw, the obtained capsule 's content sterilized water of Example 4 is mixed with 0.5,0.25,0.125g/mL, with 20mL/kgbw gavage, every day 1 time, continuous 5d.Within the 35th day after giving sample first, put to death animal, get the film-making routinely of both sides epididymal sperm filtrate, microscopy, every mouse counts 1000 complete sperms, calculates Sperm malformation rate.
30 days feeding trials: capsules in healthy volunteers recommended amounts 1.4g/ people/sky that embodiment 4 is obtained, press 30 of human body recommended amounts, 65, establish 0.70g/kgbw/d for 100 times, 1.52g/kgbw/d, 2.33g/kgbw/d establishes three dosage groups, separately establish a blank group 0g/kgbw, given the test agent mixes in basal feed rat of feeding, continuous nursing 30 days, claim 1 body weight and 2 food intake doses weekly, calculate weekly and total food utilization, every day observes with or without poisoning realization and death, hematology is observed in off-test, blood biochemical, organ coefficient index, and carry out pathological examination.
Result of the test:
1, acute oral toxicity test result: the maximal tolerance dose (MTD) of given the test agent to female male chmice acute per os is all greater than 20.0g/kgbw, belongs to nontoxic level.
2, three genetic toxicity test results: Salmonella reversion test, mouse marrow cell micro nuclear test, mouse inbred strain result are feminine gender.
3,30 days feeding trial results: experimental session, animal growth is good, each dosage group body weight, food utilization, organ weights, organ coefficient change all without exception, hematological indices and biochemical indices are all in normal range (NR), and each internal organs pathological examination is showed no does not observe illeffects to by the relevant pathological change of test product, the tested material that gives human body recommended amounts 100 times.
Test example 2
To release the pressure function test:
Test objective: whether inspection product has the function of releasing the pressure to human body
Test material: the capsule that embodiment 4 is obtained.
Sample: human oral's recommended amounts is every day 2 times, each 2, capsule No.1, No. 2, the packaging of the two, outward appearance are substantially identical, but one of them capsule that to be embodiment 4 obtained, another is placebo.
Experimenter selects: experimenter is the scientific research personnel that operating pressure is large, the age 35-55 year, male or female, allly meets above-mentioned condition and voluntary participation and ensures that cooperation person all can include test in.Get rid of following personnel: to health food allergy sufferers; Take the article relevant to tested function in a short time, have influence on the judgement to result.
Test method: when human body is in the state of pressure, will secrete cortisol in saliva, therefore can by taking salivary cortisol as the effect that pressure index evaluation tested material is released the pressure.Experimenter is divided into test-meal group and placebo group at random, and every day takes by RD, takes 4 weeks continuously.Duration of test does not change original eating habit, normal diet.As shown in Figure 2, Fig. 2 is the rate of change (starting point 100%) of subjects saliva's cortisol to result.As shown in Figure 2, compared with control group, test-meal group subjects saliva cortisol increases and significantly reduces (p<0.05), illustrates that the capsule that embodiment 4 obtains has the effect of releasing the pressure.
Test example 3
Improving memory function test:
Test objective: whether inspection product has the function of Improving memory to human body
Test material: the capsule that embodiment 4 is obtained.
Sample: human oral's recommended amounts is every day 2 times, each 2, capsule No.1, No. 2, the packaging of the two, outward appearance are substantially identical, but one of them capsule that to be embodiment 4 obtained, another is placebo.
Experimenter selects: failure of memory person, age 25-50 year, select the volunteer meeting experimental condition to take tested material 45 days, experimenter 106, be divided into each 53 of test-meal group, control group at random, at the end of test-meal, test-meal group 1 people and control group 2 people do not participate in inspection.Before and after test-meal, experimenter's spirit diet, stool and urine situation no abnormality seen.
Test method: according to " health food inspection and assessment technical specification " (2003 editions) evaluation criterion, selection memory quotient is effect of the Improving memory of metrics evaluation tested material.Adopt counter point between Self-control method and group, duration of test does not change original eating habit, normal diet.Result is as shown in table 1.
Table 1 product is on the impact of memory quotient
| Before test | After test | |
| Control group | 87.13±19.82 | 91.16±20.93 |
| Test group | 87.09±21.02 | 99.33±18.68 |
As shown in Table 1, before test-meal, the memory quotient of test group compares with control group, no significant difference (P>0.05), after test-meal, the memory quotient of test group compares with control group increase significantly (P<0.05), and not finding obvious adverse reaction during test-meal, result judges to execute effect that the obtained capsule of example 4 has auxiliary improvement of memory thus.
Claims (9)
1. release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, comprise the steps:
(1) asparagus solid fermentation: with the natural constituents containing Improving memory effect for property of medicine culture matrix, inoculation flammulina velutipes liquid strains carries out the medicinal fungal substance that the preparation of two-way solid fermentation has Improving memory effect;
The preparation of described property of medicine culture matrix: the raw material first taking following weight percentage: wholegrain barley 70%-80%, soybean 10%-20%, walnut kernel 1%-3%, fructus alpiniae oxyphyllae 1%-3%, ginkgo leaf 1%-3%, land plaster 0.5%-1.5%, by each component mixing, pulverize, then adding water to moisture weight content is 60%-65%, 116 DEG C-126 DEG C sterilizing 0.5h-1.5h;
(2) lactic acid bacteria liquid deep layer fermenting: with the medicinal fungal substance of step (1) for raw material, add dusty yeast after pulverizing, sodium glutamate, water makes fermentation medium, inoculating lactic acid bacterium carries out liquid deep layer fermenting and produces the GABA of releasing the pressure, and preparation has the zymotic fluid of releasing the pressure with Improving memory effect simultaneously;
(3) adopt the zymotic fluid of step (2), be prepared into releasing the pressure and the functional food of Improving memory of different dosage form.
2. according to claim 1ly release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (1), the inoculum concentration of described flammulina velutipes liquid strains is 2%-10%.
3. according to claim 1ly to release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (1), the preparation of described property of medicine culture matrix: the raw material first taking following weight percentage: wholegrain barley 75%, soybean 15%, walnut kernel 3%, fructus alpiniae oxyphyllae 3%, ginkgo leaf 3%, land plaster 1%, by each component mixing, pulverize, then adding water to moisture weight content is 60%-65%, 121 DEG C of sterilizing 1h.
4. according to claim 1ly to release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (1), the condition of described two-way solid fermentation is: fermentation temperature is at 20-25 DEG C, and fermentation time is at 20-30 days.
5. according to claim 1ly to release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (2), described fermentation medium adopts the raw material of following weight portion: water 1000 weight portion, medicinal fungal substance 40-50 weight portion, dusty yeast 5-10 weight portion, sodium glutamate 15-20 weight portion, by water-soluble to medicinal fungal substance, dusty yeast and sodium glutamate, interpolation acetic acid and sodium acetate are mixed with the buffer solution that pH is 5-6,116 DEG C-126 DEG C sterilizing 20min-40min.
6. according to claim 1ly release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (2), the inoculum concentration of described lactic acid bacteria is 1%-5%.
7. according to claim 1ly to release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (2), the condition of described liquid deep layer fermenting is: fermentation temperature is at 28-32 DEG C, and fermentation time is at 48-72h.
8. according to claim 1ly to release the pressure and the two-step fermentation preparation method of functional food of Improving memory, it is characterized in that, in step (2), described to release the pressure and the formulation of functional food of Improving memory power is tablet or capsule.
9. prepared by the preparation method according to any one of claim 1 ~ 8 release the pressure and the functional food of Improving memory.
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| CN104488552B (en) * | 2014-12-25 | 2017-04-12 | 皖南大鹏天然产物有限公司 | Method for producing needle mushroom mycelium by virtue of ginkgo leaf residues |
| CN109223668B (en) * | 2018-09-30 | 2021-04-27 | 上海清轩生物科技有限公司 | Preparation method and application of two-step fermentation product with skin repairing effect |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102428832A (en) * | 2011-09-14 | 2012-05-02 | 大连百祥聚生物科技有限公司 | Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof |
| CN103110057A (en) * | 2012-05-04 | 2013-05-22 | 黄家章 | Two-step fermentation preparation method and application of sweet fermented-rice nutrient or brewing nutrition products |
| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103540624A (en) * | 2012-07-17 | 2014-01-29 | 蔡国珍 | Method for preparing gamma-aminobutyric acid through fermenting cordyceps fungus in rice medium and application of method |
| CN103565944A (en) * | 2013-11-20 | 2014-02-12 | 青岛农业大学 | Bi-directional fermentation technology for traditional Chinese medicine |
-
2014
- 2014-04-04 CN CN201410136197.1A patent/CN103932181B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102428832A (en) * | 2011-09-14 | 2012-05-02 | 大连百祥聚生物科技有限公司 | Fungal pharmaceutical mycoplasm with blood sugar lowering efficacy and preparation method thereof |
| CN103110057A (en) * | 2012-05-04 | 2013-05-22 | 黄家章 | Two-step fermentation preparation method and application of sweet fermented-rice nutrient or brewing nutrition products |
| CN103540624A (en) * | 2012-07-17 | 2014-01-29 | 蔡国珍 | Method for preparing gamma-aminobutyric acid through fermenting cordyceps fungus in rice medium and application of method |
| CN103316058A (en) * | 2013-05-22 | 2013-09-25 | 甘肃农业大学 | Technology for improving drug effects of Mythic Fungus and Chinese angelica through utilizing Mythic Fungus-Chinese angelica bidirectional fermentation |
| CN103565944A (en) * | 2013-11-20 | 2014-02-12 | 青岛农业大学 | Bi-directional fermentation technology for traditional Chinese medicine |
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Denomination of invention: The invention relates to a functional food for relieving pressure and improving memory and a two-step fermentation preparation method thereof Effective date of registration: 20220111 Granted publication date: 20150909 Pledgee: Laiwu Shandong rural commercial bank Limited by Share Ltd. Pledgor: Junliantikang (Shandong) Pharmaceutical Co.,Ltd. Registration number: Y2022980000351 |
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Date of cancellation: 20230111 Granted publication date: 20150909 Pledgee: Laiwu Shandong rural commercial bank Limited by Share Ltd. Pledgor: Junliantikang (Shandong) Pharmaceutical Co.,Ltd. Registration number: Y2022980000351 |