CN103750102B - There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar - Google Patents

There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar Download PDF

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CN103750102B
CN103750102B CN201310752392.2A CN201310752392A CN103750102B CN 103750102 B CN103750102 B CN 103750102B CN 201310752392 A CN201310752392 A CN 201310752392A CN 103750102 B CN103750102 B CN 103750102B
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blueberry
blackberry
extract
trehalose
blood sugar
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CN103750102A (en
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文舜
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NANJING RUNSHOUYUAN BIOTECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to a kind of composition and method of making the same containing blackberry, blueberry with function of reducing blood sugar, said composition take blackberry, blueberry as key component, the preparation of interpolation plant extracts Compound Machining obtains, said composition steady quality, long shelf-life, not only make the fruit juice of patients with diabetes mellitus delicious food, effectively can also reduce blood sugar, be applicable to hyperglycemia population, particularly diabetic.

Description

There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar
Technical field
The present invention relates to a kind of composition containing blackberry, blueberry, relate generally to the blackberry, blueberry complex composition of beneficiating ingredient and the blood sugar decreasing effect with high-load.
Background technology
Blackberry, blueberry, belongs to berries, originates in American-European, has very high nutritive value and medical value.Its fruit is rich in the multivitamins such as Vc, Ve, Vb1, Vb2 and amino acid, the mineral matters such as very high SOD, anthocyanin and potassium, magnesium, calcium, selenium, its nutritive value is far above fruit such as apple, orange, grapes, there is the effects such as anti-ageing, anticancer, hypoglycemic, can be rated as the world " king of fruit ", occupy first of all kinds of fruit, in the U.S., hat has " life is fruit " good reputation, within 1993, food and agricultural organization of united state is recommended as third generation fruit, becomes American-European and the fruit consumption fashion in Australia.
It is reported, a series of researchs that the scientists of illinois university is carried out show, blackberry, blueberry can promote the insulin discharging sufficient amount, thus play the effect controlling blood sugar for human body index.Blackberry, blueberry contains a kind of special compound, and comprising well known AMS and alpha-glucosidase, they are that one plays inhibiting enzyme to carbohydrate.Report in addition, this university scientific man finds, berries wine is rich in beneficial activity composition, and effect is almost suitable with hypoglycemic drug acarbose (Acarbose).
As the saying goes, be medicine three points of poison.Hypoglycemic drug now used clinically, do not have one can cure diabetes completely, just regulating blood glucose levels, and these medicines all have some limitations and bad reaction, or even very serious reaction, can hypoglycemia, lactic acidosis etc. be caused.So, if dietotherapy progressively alternatives to medication treatment and the effect of drug therapy can be met or exceeded can be applied, by eliminating the toxic and side effect of diabetic's drug therapy and alleviating its economic pressures, bring glad tidings for them and wish.
Summary of the invention
The object of the invention is the defect for above-mentioned diabetes drug treatment, the composition containing blackberry, blueberry with effect of lowering blood sugar providing a kind of safety to have no side effect.
The object of the invention is to realize in the following manner:
Have the composition containing blackberry, blueberry of function of reducing blood sugar, said composition is made primarily of following raw material:
The mass ratio 20:1-2 of blackberry, blueberry and compound; Wherein, compound is (in each composition weight sum of compound for 100%) according to each component of weight percent content: Ba Naba extract 20%-35%, Bitter Melon P.E 20%-35%, blue or green money willow extract 15%-30%, guava extract 8%-25%, Radix et Caulis Opuntiae Dillenii extract 5%-25%, Arabinose 3%-10%, trehalose 2%-10%, Hericium erinaceus extract 5%-20%, Arabic gum 1 ‰-2.5 ‰.
Above-mentioned composition is preferably made primarily of following raw material:
The mass ratio 20:1 of the compound of blackberry, blueberry and plant extracts; Wherein, the compound of plant extracts according to each component of weight percent content is: Ba Naba extract 25%-30%, Bitter Melon P.E 25%-30%, blue or green money willow extract 15%-25%, guava extract 8%-15%, Radix et Caulis Opuntiae Dillenii extract 8%-15%, Arabinose 3%-5%, trehalose 2%-5%, Hericium erinaceus extract 5%-10%, Arabic gum 1 ‰-2 ‰.
The product form of described composition can be concentrate, fruit juice, jam or dry powder.
The above-mentioned preparation method with the composition containing blackberry, blueberry of function of reducing blood sugar comprises the following steps:
A), the process of blackberry, blueberry raw material: get blackberry, blueberry, add the bamboo extractive (the golden basin bio tech ltd of Dongguan City produces) of blackberry, blueberry quality 1 ‰-3 ‰ (w/w) and the mixture of green-tea extract (Xinxiang City opens derived food additive Co., Ltd and produces), the weight ratio of bamboo extractive and green-tea extract is 1:1, after mixing, making beating, sieve de-seed, and the seed taken off is ground to 120-140 orders, by its add-back blackberry, blueberry magma; Add the complex enzyme formulation of 6g/L again, enzymolysis 40-60min at 40 DEG C-50 DEG C;
B), the sugar-free preparatory stage: the invertase trehalose mixed liquor adding 1.5-5g/L in the magma after above-mentioned enzymolysis, is hydrolyzed 40-60min at 40-50 DEG C, and go out enzyme and sterilizing 5-6min; Wherein, invertase trehalose mixed liquor is made up of invertase, trehalose, pure water, and part by weight is 4:1:5;
C), sugar-free operation stage: reduce temperature to 30-35 DEG C, in above-mentioned blackberry, blueberry magma inoculate 0.5%-1%(v/v) Lactobacillus plantarum nutrient solution, fully mix, pass into filtrated air, and Keep agitation 4-10h, detect between 0 °-0.3 ° (now the viable count of the turbid juice of blackberry, blueberry is not higher than 1 × 103cfu/mL) to blackberry, blueberry turbid juice pol, then sterilization 5-6min;
D) it is, concentrated filling: under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrated above-mentioned sterilization to pH2-3(now cycles of concentration be 5-6 times), under agitation, add trehalose, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum successively, sterile filling, sealing, sterilization 5-6min, is cooled to normal temperature.When compound adds, often add after a kind of component mixes and add lower a kind of material again.
Described complex enzyme formulation is account for the pectase of complex enzyme formulation weight 80%, 15% cellulase, 4% hemicellulase and 1% protease.Described invertase trehalose mixed liquor is mixed formed by invertase, trehalose, pure water, and three's part by weight is 4:1:5.
Step b in said method) in go out enzyme and sterilizing and step c) and d) in sterilization temperature all not higher than 85 DEG C.
In said method, the preparation of Lactobacillus plantarum nutrient solution: buy delicatessen food level Lactobacillus plantarum (Chinese industrial Culture Collection CICC buys), in vitro 24h is activated under applying conventional MRS culture medium 30-35 DEG C of condition, inoculum concentration 0.5%, Anaerobic culturel 24h under 30-35 DEG C of condition again, is mixed with the cell suspending liquid that viable count is 1 × 106-1 × 107cfu/mL.
In the composite formulations that the present invention is used: Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Arabinose, trehalose, Hericium erinaceus extract, Arabic gum are commercially available prod.
Ba Naba extract: active ingredient is Corosolic acid, is called as glucokinin, to diabetes and obesity effect remarkable.Bitter Melon P.E: balsam pear contains steroid saponin, as Charantin, para-insulin peptide class and alkaloid, it is active that these materials give balsam pear hypoglycemic, so balsam pear has antidiabetic effect, in addition, also has antiviral functions.Blue or green money willow extract: blue or green money willow is Chinese distinctive natural health care resource, is called as botanic 3rd tree, has the remarkable efficacy such as hypoglycemic, lipopenicillinase, step-down.Guava extract: guava is nutritious, vitamin C, iron content are extra-high, and fruit has treatment diabetes and hypoglycemic effect, is the desirable fruit of obesity patient and stomach gastrointestinal disease patient.Radix et Caulis Opuntiae Dillenii extract: the triterpenoid saponin that cactus contains and multiple Flavonoid substances, cruel friend's fat-reducing and obvious hypoglycemic activity, and also cactus also has inhibiting bacteria and diminishing inflammation effect.Arabinose: it is a kind of sugar low in calories, can affect the invertase digesting sucrose in small intestine disaccharide-hydrolysing enzymes selectively, thus the absorption suppressing sucrose.Trehalose: it is a kind of safe and reliable natural carbohydrate; it can form unique diaphragm at cell surface under the severe environmental conditions such as high temperature, high and cold, hyperosmosis and dry dehydration; protected protein matter molecule consistency inactivation effectively, thus the life process of the body that sustains life and biological characteristic.Hericium erinaceus extract: active component is Hericium erinaceus Polysaccharides and erinacine, nourishing the stomach can protect stomach, nourishing qi and blood etc., is the raw material of desirable medicine, health products, nutriment, food and drink etc.Arabic gum: as a kind of stabilizing agent, be widely used in food industry.
Beneficial effect of the present invention compared with the prior art:
1, Lactobacillus plantarum can produce Plantaricin by L. plantarum in growth course, the present invention can when not adding any anticorrisive agent its shelf-life of significant prolongation.
2, the blackberry drink prepared by the inventive method is placed after 18 months and is occurred without precipitation or research of chaotic phenomenon; and prior art usually can the anthocyanidin of large losses for the processing procedure of blackberry, blueberry, and by the inventive method process obtain substantially constant containing its active ingredient anthocyanidin content in the composition of blackberry, blueberry.
3, the present invention is on the basis of the original effect of lowering blood sugar of blackberry, blueberry, by adding the compound had no side effect, significantly strengthens its effect of lowering blood sugar.
4, the compound mainly plant extracts that the present invention is used, its wide material sources, green, healthy, have no side effect.Wherein include hypoglycemic plant extracts and also there is the effect that stomach is protected in nourishing the stomach, prevent hypoglycemic stimulation stomach generation simultaneously and produce bad reactions such as feeling sick, feel bad.
Therefore, the present invention is a kind of safety and effectively can falls the hypoglycemic composition product containing blackberry, blueberry, functional food and field of beverage can be applied to, comprise the industries such as health products, can progressively realize with dietotherapy alternative medicine treatment diabetes, eliminate the toxic and side effect of drug therapy, alleviate economic pressures and the burden of diabetes patient.
Detailed description of the invention
Explained in details the present invention is carried out below by way of specific embodiment, but not as the restriction to content of the present invention.
Wherein, Ba Naba extract (Hunan Yuanhang Biological Technology Co., Ltd.'s production), Bitter Melon P.E (production of Kang Ermei bio tech ltd, Xi'an), blue or green money willow extract (Chenzhou Miroherb Biological Products Co., Ltd.'s production), guava extract (production of Zhengzhou, Henan Province Li Nuo bio tech ltd), Radix et Caulis Opuntiae Dillenii extract's (production of Tongjiang, Xi'an biotechnology Co., Ltd), Hericium erinaceus extract (upper Haikang boat fungi polysaccharide Co., Ltd produces), bamboo extractive (the golden basin bio tech ltd of Dongguan City produces), green-tea extract (Xinxiang City opens derived food additive Co., Ltd and produces).Pol is counted: LH-T10 Refractometer, and Hangzhou Lu Heng Science and Technology Ltd. manufactures.
Embodiment 1
Formula: blackberry, blueberry 100kg, compound 6kg
The each composition weight of compound:
Take 100kg blackberry, blueberry and freeze fruit, removal of impurities is cleaned after low temperature thaws, add bamboo extractive and the green-tea extract (1:1 of 0.3kg, w/w) color retention is carried out, application beater carries out making beating process, sieve de-seed, its mesh size is between 0.6-0.8mm, to join in blackberry, blueberry magma with continuous 120-140 object seeds that are ground to for three times of colloid mill again, (complex enzyme formulation is the pectase accounting for complex enzyme formulation weight 80% to add 6g/L complex enzyme formulation, 15% cellulase, 4% hemicellulase and 1% proteinase mixture), in 45 DEG C of enzymolysis 50min, in the magma after above-mentioned enzymolysis, adding 3g/L invertase trehalose mixed liquor, (invertase trehalose mixed liquor is by invertase, trehalose, pure water mixing composition, part by weight is 4:1:5.), in 50 DEG C of hydrolysis 50min, application Conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, reduce temperature to 35 DEG C and obtain blackberry, blueberry magma, to cooling after blackberry, blueberry magma in inoculate 1%(v/v) Lactobacillus plantarum nutrient solution, fully mix immediately, pass into filtrated air, and Keep agitation 6h, now the turbid juice saccharometer of blackberry, blueberry detects the position that scale maintains 0.2 ° substantially, again with conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrated above-mentioned sterilization to pH2.5(now cycles of concentration be 5-6 times), its density is suitable, mobility is better, again under agitation, add trehalose successively, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum, (being all after stirring, add lower a kind of material again) at every turn, filling, sealing, last ultraviolet microwave integration apparatus less than 85 DEG C sterilization 5-6min using routine again, under room temperature, be cooled to normal temperature obtain.
Inspection: by country fruit, vegetables juice beverages health standard (GB19297-2003), immediately to carrying out sense organ and physics and chemistry, bioassay containing the concentrate product of blackberry, blueberry, reject and leak tank, be up to the standards, coding vanning list marketing, the shelf-life is 18 months.
Through inspection, the content of arsenic, lead, copper and total plate count in product, coli-group, pathogenic bacteria all meet national standard: the index really, in vegetables juice beverages health standard (GB19297-2003).Further, place after 18 months and occur without precipitation or research of chaotic phenomenon, and the anthocyanidin content detected wherein is blackberry, blueberry before treatment 96.5%.
Test of pesticide effectiveness example
Animal used as test: Wistar rat 120, male, 180-200g.Rat adapts to nursing after 5 days at Animal House, and randomly draw 10 Normal groups and feed with common maintenance feed, experimental group is fed with high glucose and high fat feed, high glucose and high fat feed formula: rat basal feed 67%, sucrose 11%, yolk powder 9%, lard 8%, cholesterol 1%, cholate 0.2%.
Embodiment: all the other 110 adopt high glucose and high fat forage feed 4 weeks.After 4 weeks, rat is after fasting 18h, Streptozotocin intravenous injection 20mg/kg, and every two days once, and after using twice, the blood pressure and blood lipoid person of remaining high is modeling success.Choose the successful rat of modeling 100, be divided into 5 groups at random, often organize 10, first group is model group, does not namely feed with any medicine or hypoglycemic food; Feed with acarbose hypoglycemic drug for second group, dosage is: 50mg once-a-day 2 days → 50mg, twice on the mono-2 days → 50mg, three times on the mono-3 days → 100mg three times on the mono-, takes before the meal; 3rd group feed with blackberry, blueberry magma, dosage: 15mL/kg once, three times on the one; 4th group feed with blackberry, blueberry sugar-free concentrate, dosage 20mL/kg once, three times on the one; Feed Israel and Palestine for 5th group and take bar extract, the same acarbose of dosage; Feed with Bitter Melon P.E for 6th group, dosage is the same; Feed with blue or green money willow extract for 7th group, dosage is the same; Feed with guava extract for 8th group, dosage is the same; Feed with Radix et Caulis Opuntiae Dillenii extract for 9th group, dosage is the same; The tenth group of composition product containing blackberry, blueberry of feeding with the embodiment of the present invention 1, dosage is with the 3rd group; Take 20 days continuously.
Statistical analysis: data information adopts x ± s, uses SPSS19.0 statistical software to carry out data analysis, compares employing variance analysis between many groups, compares and adopt t inspection between two groups.P<0.05 has statistical significance.
Curative effect: have detected fasting blood-glucose (for FPG), 2h-plasma glucose (2hPG) biochemical indicator, unit is mmol/L.
Group FPG 2hPG
Normal group 5.26±0.25 7.67±0.22
Model group 20.52±3.98 26.31±4.01
Medicine group 7.35±0.95 8.15±0.86
Blackberry, blueberry magma group 15.28±1.02 19.12±1.21
Blackberry, blueberry concentrate group 11.83±0.56 14.61±0.62
Ba Naba extract 15.01±3.26 23.23±4.13
Bitter Melon P.E 17.94±4.23 24.03±3.25
Blue or green money willow extract 18.46±2.01 23.65±2.75
Guava extract 16.73±4.56 24.37±3.68
Radix et Caulis Opuntiae Dillenii extract 17.83±2.66 24.21±3.53
Product group of the present invention 6.73±0.32 7.92±0.47
Conclusion: on the impact of FPG and 2hPG as seen from the above table, except normal group, model group FPG, 2hPG all obviously raise, comparatively model group is compared, blackberry, blueberry magma group and blackberry, blueberry concentrate group FPG, 2hPG declines all to some extent, but blood sugar concentration is still in higher level, medicine group and product group FPG of the present invention, 2hPG but has obvious decline (P<0.01), product group of the present invention is significantly better than medicine group (P<0.05), also blackberry, blueberry magma group (P<0.01) is better than, blackberry, blueberry concentrate group (P<0.01), Ba Naba extract group (P<0.01), Bitter Melon P.E group (P<0.01), blue or green money willow extract group (P<0.01), guava extract group (P<0.01) and Radix et Caulis Opuntiae Dillenii extract's group (P<0.01).It can thus be appreciated that the blood sugar reducing function of the present invention's combination is not the simple addition of each constituent function, is the synergistic function of each constituent.
Clinical testing example:
Data and method: physical data outpatient service type ii diabetes patient is totally 100 examples, wherein man 59 example, female 41 example, patient age 51-78 years old, fasting blood-glucose (FPG) and 2h-plasma glucose (2hPG) all significantly raise.The hypoglycemic concentrate of blackberry, blueberry sugar-free taking the embodiment of the present invention 1 preparation, after one month, detects above-mentioned biochemical indicator.Cure: FPG<6.1mmol/L, 2h blood sugar (2hPG) <8.0mmol/L.Invalid: fasting blood-glucose (FPG), 2h-plasma glucose (2hPG) are all without obviously changing.Effective: fasting blood-glucose (FPG), 2h-plasma glucose (2hPG) make moderate progress, but do not reach normal level.
Result for the treatment of: cure 71 examples, effective 16 examples, invalid 3 examples, total effective rate is 97%.
Embodiment 2
Formula: blackberry, blueberry 100kg, compound 10kg
Complex components weight:
Take 100kg blackberry, blueberry and freeze fruit, removal of impurities is cleaned after low temperature thaws, add bamboo extractive and the green-tea extract (1:1 of 0.1kg, w/w) color retention is carried out, application beater carries out making beating process, sieve de-seed, its mesh size is between 0.6-0.8mm, to join in blackberry, blueberry magma with continuous 120-140 object seeds that are ground to for three times of colloid mill again, (complex enzyme formulation is the pectase accounting for complex enzyme formulation weight 80% to add 6g/L complex enzyme formulation, 15% cellulase, 4% hemicellulase and 1% proteinase mixture), in 45 DEG C of enzymolysis 50min, in the magma after above-mentioned enzymolysis, adding 1.5g/L invertase trehalose mixed liquor, (invertase trehalose mixed liquor is by invertase, trehalose, pure water mixing composition, part by weight is 4:1:5.), in 50 DEG C of hydrolysis 50min, application Conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, reduce temperature to 35 DEG C and obtain blackberry, blueberry magma, to cooling after blackberry, blueberry magma in inoculate 0.5%(v/v) Lactobacillus plantarum nutrient solution, fully mix immediately, pass into filtrated air, and Keep agitation 4h, now the turbid juice saccharometer of blackberry, blueberry detects the position that scale maintains 0.2 ° substantially, again with conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrated above-mentioned sterilization to pH3(now cycles of concentration be 5-6 times), its density is suitable, mobility is better, again under agitation, add trehalose successively, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum, (being all after stirring, add lower a kind of material again) at every turn, filling, sealing, last ultraviolet microwave integration apparatus less than 85 DEG C sterilization 5-6min using routine again, under room temperature, be cooled to normal temperature obtain.Inspection: after sterilization cooling, by country fruit, vegetables juice beverages health standard (GB19297-2003), carries out sense organ and physics and chemistry, bioassay to blackberry, blueberry sugar-free concentrate product immediately, rejects and leak tank, be up to the standards, and coding vanning list marketing, the shelf-life is 18 months.
Through inspection, the content of arsenic, lead, copper and total plate count in product, coli-group, pathogenic bacteria all meet national standard: the index really, in vegetables juice beverages health standard (GB19297-2003).Further, place after 18 months and occur without precipitation or research of chaotic phenomenon, the anthocyanidin content detected wherein is blackberry, blueberry before treatment 95.7%.

Claims (7)

1. there is the composition containing blackberry, blueberry of function of reducing blood sugar, it is characterized in that said composition is made primarily of following raw material:
The mass ratio 20:1-2 of blackberry, blueberry and compound; Wherein, compound according to each component of weight percent content is: Ba Naba extract 20%-35%, Bitter Melon P.E 20%-35%, blue or green money willow extract 15%-30%, guava extract 8%-25%, Radix et Caulis Opuntiae Dillenii extract 5%-25%, Arabinose 3%-10%, trehalose 2%-10%, Hericium erinaceus extract 5%-20%, Arabic gum 1 ‰-2.5 ‰;
Said composition is prepared by following steps:
A), the process of blackberry, blueberry raw material: get blackberry, blueberry, add the bamboo extractive of blackberry, blueberry quality 1 ‰-3 ‰ and the mixture of green-tea extract, the weight ratio of bamboo extractive and green-tea extract is 1:1, after mixing, making beating, sieve de-seed, and the seed taken off is ground to 120-140 orders, by its add-back blackberry, blueberry magma; Add the complex enzyme formulation of 6g/L again, enzymolysis 40-60min at 40 DEG C-50 DEG C;
B), the sugar-free preparatory stage: the invertase trehalose mixed liquor adding 1.5-5g/L in the magma after above-mentioned enzymolysis, is hydrolyzed 40-60min at 40-50 DEG C, and go out enzyme and sterilizing 5-6min; Wherein, invertase trehalose mixed liquor is made up of invertase, trehalose, pure water, and part by weight is 4:1:5;
C), sugar-free operation stage: reduce temperature to 30-35 DEG C, inoculate the Lactobacillus plantarum nutrient solution of 0.5%-1% in above-mentioned blackberry, blueberry magma, fully mix, pass into filtrated air, and Keep agitation 4-10h, detect between 0 °-0.3 ° to the turbid juice pol of blackberry, blueberry, then sterilization 5-6min;
D) it is, concentrated filling: under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrating sterilizing is to pH2-3, under agitation, add trehalose, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum successively, sterile filling, sealing, sterilization 5-6min, is cooled to normal temperature.
2. the composition containing blackberry, blueberry with function of reducing blood sugar according to claim 1, is characterized in that said composition is made primarily of following raw material:
The mass ratio 20:1 of the compound of blackberry, blueberry and plant extracts; Wherein, the compound of plant extracts according to each component of weight percent content is: Ba Naba extract 25%-30%, Bitter Melon P.E 25%-30%, blue or green money willow extract 15%-25%, guava extract 8%-15%, Radix et Caulis Opuntiae Dillenii extract 8%-15%, Arabinose 3%-5%, trehalose 2%-5%, Hericium erinaceus extract 5%-10%, Arabic gum 1 ‰-2 ‰.
3. the composition containing blackberry, blueberry with function of reducing blood sugar according to claim 1, is characterized in that the product form of described composition is concentrate, fruit juice, jam or dry powder.
4. the preparation method with the composition containing blackberry, blueberry of function of reducing blood sugar described in claim 1 or 2, is characterized in that the method comprises the following steps:
A), the process of blackberry, blueberry raw material: get blackberry, blueberry, add the bamboo extractive of blackberry, blueberry quality 1 ‰-3 ‰ and the mixture of green-tea extract, the weight ratio of bamboo extractive and green-tea extract is 1:1, after mixing, making beating, sieve de-seed, and the seed taken off is ground to 120-140 orders, by its add-back blackberry, blueberry magma; Add the complex enzyme formulation of 6g/L again, enzymolysis 40-60min at 40 DEG C-50 DEG C;
B), the sugar-free preparatory stage: the invertase trehalose mixed liquor adding 1.5-5g/L in the magma after above-mentioned enzymolysis, is hydrolyzed 40-60min at 40-50 DEG C, and go out enzyme and sterilizing 5-6min; Wherein, invertase trehalose mixed liquor is made up of invertase, trehalose, pure water, and part by weight is 4:1:5;
C), sugar-free operation stage: reduce temperature to 30-35 DEG C, inoculate the Lactobacillus plantarum nutrient solution of 0.5%-1% in above-mentioned blackberry, blueberry magma, fully mix, pass into filtrated air, and Keep agitation 4-10h, detect between 0 °-0.3 ° to the turbid juice pol of blackberry, blueberry, then sterilization 5-6min;
D) it is, concentrated filling: under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrating sterilizing is to pH2-3, under agitation, add trehalose, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum successively, sterile filling, sealing, sterilization 5-6min, is cooled to normal temperature.
5. the preparation method of composition containing blackberry, blueberry with function of reducing blood sugar according to claim 4, is characterized in that described complex enzyme formulation is account for the pectase of complex enzyme formulation weight 80%, 15% cellulase, 4% hemicellulase and 1% protease.
6. the preparation method of composition containing blackberry, blueberry with function of reducing blood sugar according to claim 4, is characterized in that described step b) in go out enzyme and sterilizing and step c) and d) in sterilization temperature all not higher than 85 DEG C.
7. the preparation method with the composition containing blackberry, blueberry of effect of lowering blood sugar according to claim 4, when it is characterized in that compound adds, often adds after a kind of component mixes and adds lower a kind of material again.
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