CN103750102B - There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar - Google Patents
There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar Download PDFInfo
- Publication number
- CN103750102B CN103750102B CN201310752392.2A CN201310752392A CN103750102B CN 103750102 B CN103750102 B CN 103750102B CN 201310752392 A CN201310752392 A CN 201310752392A CN 103750102 B CN103750102 B CN 103750102B
- Authority
- CN
- China
- Prior art keywords
- blueberry
- blackberry
- extract
- trehalose
- blood sugar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000078534 Vaccinium myrtillus Species 0.000 title claims abstract description 155
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 77
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 77
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 77
- 239000000203 mixture Substances 0.000 title claims abstract description 56
- 239000008280 blood Substances 0.000 title claims abstract description 24
- 210000004369 blood Anatomy 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 239000000419 plant extract Substances 0.000 claims abstract description 7
- 235000015203 fruit juice Nutrition 0.000 claims abstract description 3
- 235000017848 Rubus fruticosus Nutrition 0.000 claims description 75
- 239000000284 extract Substances 0.000 claims description 72
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 28
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 28
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 28
- 230000001954 sterilising effect Effects 0.000 claims description 25
- 108090000790 Enzymes Proteins 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 20
- 229940088598 enzyme Drugs 0.000 claims description 20
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 18
- 235000011073 invertase Nutrition 0.000 claims description 18
- 239000001573 invertase Substances 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 18
- 241000508269 Psidium Species 0.000 claims description 16
- 241000124033 Salix Species 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 14
- 238000009472 formulation Methods 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- 240000000588 Hericium erinaceus Species 0.000 claims description 13
- 235000007328 Hericium erinaceus Nutrition 0.000 claims description 13
- 235000010489 acacia gum Nutrition 0.000 claims description 11
- 239000001785 acacia senegal l. willd gum Substances 0.000 claims description 11
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims description 11
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 11
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 11
- 238000013019 agitation Methods 0.000 claims description 10
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 9
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 9
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 9
- 244000082204 Phyllostachys viridis Species 0.000 claims description 9
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 9
- 239000011425 bamboo Substances 0.000 claims description 9
- 239000012141 concentrate Substances 0.000 claims description 9
- 229940094952 green tea extract Drugs 0.000 claims description 9
- 235000020688 green tea extract Nutrition 0.000 claims description 9
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000010009 beating Methods 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 229940059442 hemicellulase Drugs 0.000 claims description 4
- 108010002430 hemicellulase Proteins 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 238000012859 sterile filling Methods 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 6
- 201000001421 hyperglycemia Diseases 0.000 abstract 1
- 238000003754 machining Methods 0.000 abstract 1
- 235000021029 blackberry Nutrition 0.000 description 52
- 235000013399 edible fruits Nutrition 0.000 description 12
- 239000008103 glucose Substances 0.000 description 12
- 230000002218 hypoglycaemic effect Effects 0.000 description 11
- 239000003814 drug Substances 0.000 description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 230000036541 health Effects 0.000 description 7
- 230000010354 integration Effects 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 6
- 238000007689 inspection Methods 0.000 description 5
- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 description 4
- 241000700159 Rattus Species 0.000 description 4
- 229960002632 acarbose Drugs 0.000 description 4
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 description 4
- 229930014669 anthocyanidin Natural products 0.000 description 4
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 4
- 235000008758 anthocyanidins Nutrition 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 244000302512 Momordica charantia Species 0.000 description 3
- 235000009811 Momordica charantia Nutrition 0.000 description 3
- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 3
- 235000018365 Momordica dioica Nutrition 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 239000003472 antidiabetic agent Substances 0.000 description 3
- 230000000739 chaotic effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000002651 drug therapy Methods 0.000 description 3
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 3
- 231100000957 no side effect Toxicity 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000219357 Cactaceae Species 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 208000008589 Obesity Diseases 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 229910052785 arsenic Inorganic materials 0.000 description 2
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000021028 berry Nutrition 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 235000020824 obesity Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 235000019833 protease Nutrition 0.000 description 2
- 210000000697 sensory organ Anatomy 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- FRWNAQDBODEVAL-VMPITWQZSA-N (5e)-5-[(4-nitrophenyl)methylidene]-2-sulfanylidene-1,3-thiazolidin-4-one Chemical group C1=CC([N+](=O)[O-])=CC=C1\C=C\1C(=O)NC(=S)S/1 FRWNAQDBODEVAL-VMPITWQZSA-N 0.000 description 1
- QNMKGMUGYVWVFQ-UHFFFAOYSA-N 2alpha-Hydroxyursolic acid Natural products CC12CC(O)C(O)C(C)(C)C1CCC1(C)C2CC=C2C3C(C)C(C)(C)CCC3(C(O)=O)CCC21C QNMKGMUGYVWVFQ-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003178 anti-diabetic effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229930185803 charantin Natural products 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229930191277 erinacine Natural products 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 235000020983 fruit intake Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 208000018685 gastrointestinal system disease Diseases 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 208000006443 lactic acidosis Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 150000005856 steroid saponins Chemical class 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
- 229960001052 streptozocin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000010353 tongjiang Substances 0.000 description 1
- 150000008130 triterpenoid saponins Chemical class 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to a kind of composition and method of making the same containing blackberry, blueberry with function of reducing blood sugar, said composition take blackberry, blueberry as key component, the preparation of interpolation plant extracts Compound Machining obtains, said composition steady quality, long shelf-life, not only make the fruit juice of patients with diabetes mellitus delicious food, effectively can also reduce blood sugar, be applicable to hyperglycemia population, particularly diabetic.
Description
Technical field
The present invention relates to a kind of composition containing blackberry, blueberry, relate generally to the blackberry, blueberry complex composition of beneficiating ingredient and the blood sugar decreasing effect with high-load.
Background technology
Blackberry, blueberry, belongs to berries, originates in American-European, has very high nutritive value and medical value.Its fruit is rich in the multivitamins such as Vc, Ve, Vb1, Vb2 and amino acid, the mineral matters such as very high SOD, anthocyanin and potassium, magnesium, calcium, selenium, its nutritive value is far above fruit such as apple, orange, grapes, there is the effects such as anti-ageing, anticancer, hypoglycemic, can be rated as the world " king of fruit ", occupy first of all kinds of fruit, in the U.S., hat has " life is fruit " good reputation, within 1993, food and agricultural organization of united state is recommended as third generation fruit, becomes American-European and the fruit consumption fashion in Australia.
It is reported, a series of researchs that the scientists of illinois university is carried out show, blackberry, blueberry can promote the insulin discharging sufficient amount, thus play the effect controlling blood sugar for human body index.Blackberry, blueberry contains a kind of special compound, and comprising well known AMS and alpha-glucosidase, they are that one plays inhibiting enzyme to carbohydrate.Report in addition, this university scientific man finds, berries wine is rich in beneficial activity composition, and effect is almost suitable with hypoglycemic drug acarbose (Acarbose).
As the saying goes, be medicine three points of poison.Hypoglycemic drug now used clinically, do not have one can cure diabetes completely, just regulating blood glucose levels, and these medicines all have some limitations and bad reaction, or even very serious reaction, can hypoglycemia, lactic acidosis etc. be caused.So, if dietotherapy progressively alternatives to medication treatment and the effect of drug therapy can be met or exceeded can be applied, by eliminating the toxic and side effect of diabetic's drug therapy and alleviating its economic pressures, bring glad tidings for them and wish.
Summary of the invention
The object of the invention is the defect for above-mentioned diabetes drug treatment, the composition containing blackberry, blueberry with effect of lowering blood sugar providing a kind of safety to have no side effect.
The object of the invention is to realize in the following manner:
Have the composition containing blackberry, blueberry of function of reducing blood sugar, said composition is made primarily of following raw material:
The mass ratio 20:1-2 of blackberry, blueberry and compound; Wherein, compound is (in each composition weight sum of compound for 100%) according to each component of weight percent content: Ba Naba extract 20%-35%, Bitter Melon P.E 20%-35%, blue or green money willow extract 15%-30%, guava extract 8%-25%, Radix et Caulis Opuntiae Dillenii extract 5%-25%, Arabinose 3%-10%, trehalose 2%-10%, Hericium erinaceus extract 5%-20%, Arabic gum 1 ‰-2.5 ‰.
Above-mentioned composition is preferably made primarily of following raw material:
The mass ratio 20:1 of the compound of blackberry, blueberry and plant extracts; Wherein, the compound of plant extracts according to each component of weight percent content is: Ba Naba extract 25%-30%, Bitter Melon P.E 25%-30%, blue or green money willow extract 15%-25%, guava extract 8%-15%, Radix et Caulis Opuntiae Dillenii extract 8%-15%, Arabinose 3%-5%, trehalose 2%-5%, Hericium erinaceus extract 5%-10%, Arabic gum 1 ‰-2 ‰.
The product form of described composition can be concentrate, fruit juice, jam or dry powder.
The above-mentioned preparation method with the composition containing blackberry, blueberry of function of reducing blood sugar comprises the following steps:
A), the process of blackberry, blueberry raw material: get blackberry, blueberry, add the bamboo extractive (the golden basin bio tech ltd of Dongguan City produces) of blackberry, blueberry quality 1 ‰-3 ‰ (w/w) and the mixture of green-tea extract (Xinxiang City opens derived food additive Co., Ltd and produces), the weight ratio of bamboo extractive and green-tea extract is 1:1, after mixing, making beating, sieve de-seed, and the seed taken off is ground to 120-140 orders, by its add-back blackberry, blueberry magma; Add the complex enzyme formulation of 6g/L again, enzymolysis 40-60min at 40 DEG C-50 DEG C;
B), the sugar-free preparatory stage: the invertase trehalose mixed liquor adding 1.5-5g/L in the magma after above-mentioned enzymolysis, is hydrolyzed 40-60min at 40-50 DEG C, and go out enzyme and sterilizing 5-6min; Wherein, invertase trehalose mixed liquor is made up of invertase, trehalose, pure water, and part by weight is 4:1:5;
C), sugar-free operation stage: reduce temperature to 30-35 DEG C, in above-mentioned blackberry, blueberry magma inoculate 0.5%-1%(v/v) Lactobacillus plantarum nutrient solution, fully mix, pass into filtrated air, and Keep agitation 4-10h, detect between 0 °-0.3 ° (now the viable count of the turbid juice of blackberry, blueberry is not higher than 1 × 103cfu/mL) to blackberry, blueberry turbid juice pol, then sterilization 5-6min;
D) it is, concentrated filling: under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrated above-mentioned sterilization to pH2-3(now cycles of concentration be 5-6 times), under agitation, add trehalose, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum successively, sterile filling, sealing, sterilization 5-6min, is cooled to normal temperature.When compound adds, often add after a kind of component mixes and add lower a kind of material again.
Described complex enzyme formulation is account for the pectase of complex enzyme formulation weight 80%, 15% cellulase, 4% hemicellulase and 1% protease.Described invertase trehalose mixed liquor is mixed formed by invertase, trehalose, pure water, and three's part by weight is 4:1:5.
Step b in said method) in go out enzyme and sterilizing and step c) and d) in sterilization temperature all not higher than 85 DEG C.
In said method, the preparation of Lactobacillus plantarum nutrient solution: buy delicatessen food level Lactobacillus plantarum (Chinese industrial Culture Collection CICC buys), in vitro 24h is activated under applying conventional MRS culture medium 30-35 DEG C of condition, inoculum concentration 0.5%, Anaerobic culturel 24h under 30-35 DEG C of condition again, is mixed with the cell suspending liquid that viable count is 1 × 106-1 × 107cfu/mL.
In the composite formulations that the present invention is used: Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Arabinose, trehalose, Hericium erinaceus extract, Arabic gum are commercially available prod.
Ba Naba extract: active ingredient is Corosolic acid, is called as glucokinin, to diabetes and obesity effect remarkable.Bitter Melon P.E: balsam pear contains steroid saponin, as Charantin, para-insulin peptide class and alkaloid, it is active that these materials give balsam pear hypoglycemic, so balsam pear has antidiabetic effect, in addition, also has antiviral functions.Blue or green money willow extract: blue or green money willow is Chinese distinctive natural health care resource, is called as botanic 3rd tree, has the remarkable efficacy such as hypoglycemic, lipopenicillinase, step-down.Guava extract: guava is nutritious, vitamin C, iron content are extra-high, and fruit has treatment diabetes and hypoglycemic effect, is the desirable fruit of obesity patient and stomach gastrointestinal disease patient.Radix et Caulis Opuntiae Dillenii extract: the triterpenoid saponin that cactus contains and multiple Flavonoid substances, cruel friend's fat-reducing and obvious hypoglycemic activity, and also cactus also has inhibiting bacteria and diminishing inflammation effect.Arabinose: it is a kind of sugar low in calories, can affect the invertase digesting sucrose in small intestine disaccharide-hydrolysing enzymes selectively, thus the absorption suppressing sucrose.Trehalose: it is a kind of safe and reliable natural carbohydrate; it can form unique diaphragm at cell surface under the severe environmental conditions such as high temperature, high and cold, hyperosmosis and dry dehydration; protected protein matter molecule consistency inactivation effectively, thus the life process of the body that sustains life and biological characteristic.Hericium erinaceus extract: active component is Hericium erinaceus Polysaccharides and erinacine, nourishing the stomach can protect stomach, nourishing qi and blood etc., is the raw material of desirable medicine, health products, nutriment, food and drink etc.Arabic gum: as a kind of stabilizing agent, be widely used in food industry.
Beneficial effect of the present invention compared with the prior art:
1, Lactobacillus plantarum can produce Plantaricin by L. plantarum in growth course, the present invention can when not adding any anticorrisive agent its shelf-life of significant prolongation.
2, the blackberry drink prepared by the inventive method is placed after 18 months and is occurred without precipitation or research of chaotic phenomenon; and prior art usually can the anthocyanidin of large losses for the processing procedure of blackberry, blueberry, and by the inventive method process obtain substantially constant containing its active ingredient anthocyanidin content in the composition of blackberry, blueberry.
3, the present invention is on the basis of the original effect of lowering blood sugar of blackberry, blueberry, by adding the compound had no side effect, significantly strengthens its effect of lowering blood sugar.
4, the compound mainly plant extracts that the present invention is used, its wide material sources, green, healthy, have no side effect.Wherein include hypoglycemic plant extracts and also there is the effect that stomach is protected in nourishing the stomach, prevent hypoglycemic stimulation stomach generation simultaneously and produce bad reactions such as feeling sick, feel bad.
Therefore, the present invention is a kind of safety and effectively can falls the hypoglycemic composition product containing blackberry, blueberry, functional food and field of beverage can be applied to, comprise the industries such as health products, can progressively realize with dietotherapy alternative medicine treatment diabetes, eliminate the toxic and side effect of drug therapy, alleviate economic pressures and the burden of diabetes patient.
Detailed description of the invention
Explained in details the present invention is carried out below by way of specific embodiment, but not as the restriction to content of the present invention.
Wherein, Ba Naba extract (Hunan Yuanhang Biological Technology Co., Ltd.'s production), Bitter Melon P.E (production of Kang Ermei bio tech ltd, Xi'an), blue or green money willow extract (Chenzhou Miroherb Biological Products Co., Ltd.'s production), guava extract (production of Zhengzhou, Henan Province Li Nuo bio tech ltd), Radix et Caulis Opuntiae Dillenii extract's (production of Tongjiang, Xi'an biotechnology Co., Ltd), Hericium erinaceus extract (upper Haikang boat fungi polysaccharide Co., Ltd produces), bamboo extractive (the golden basin bio tech ltd of Dongguan City produces), green-tea extract (Xinxiang City opens derived food additive Co., Ltd and produces).Pol is counted: LH-T10 Refractometer, and Hangzhou Lu Heng Science and Technology Ltd. manufactures.
Embodiment 1
Formula: blackberry, blueberry 100kg, compound 6kg
The each composition weight of compound:
Take 100kg blackberry, blueberry and freeze fruit, removal of impurities is cleaned after low temperature thaws, add bamboo extractive and the green-tea extract (1:1 of 0.3kg, w/w) color retention is carried out, application beater carries out making beating process, sieve de-seed, its mesh size is between 0.6-0.8mm, to join in blackberry, blueberry magma with continuous 120-140 object seeds that are ground to for three times of colloid mill again, (complex enzyme formulation is the pectase accounting for complex enzyme formulation weight 80% to add 6g/L complex enzyme formulation, 15% cellulase, 4% hemicellulase and 1% proteinase mixture), in 45 DEG C of enzymolysis 50min, in the magma after above-mentioned enzymolysis, adding 3g/L invertase trehalose mixed liquor, (invertase trehalose mixed liquor is by invertase, trehalose, pure water mixing composition, part by weight is 4:1:5.), in 50 DEG C of hydrolysis 50min, application Conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, reduce temperature to 35 DEG C and obtain blackberry, blueberry magma, to cooling after blackberry, blueberry magma in inoculate 1%(v/v) Lactobacillus plantarum nutrient solution, fully mix immediately, pass into filtrated air, and Keep agitation 6h, now the turbid juice saccharometer of blackberry, blueberry detects the position that scale maintains 0.2 ° substantially, again with conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrated above-mentioned sterilization to pH2.5(now cycles of concentration be 5-6 times), its density is suitable, mobility is better, again under agitation, add trehalose successively, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum, (being all after stirring, add lower a kind of material again) at every turn, filling, sealing, last ultraviolet microwave integration apparatus less than 85 DEG C sterilization 5-6min using routine again, under room temperature, be cooled to normal temperature obtain.
Inspection: by country fruit, vegetables juice beverages health standard (GB19297-2003), immediately to carrying out sense organ and physics and chemistry, bioassay containing the concentrate product of blackberry, blueberry, reject and leak tank, be up to the standards, coding vanning list marketing, the shelf-life is 18 months.
Through inspection, the content of arsenic, lead, copper and total plate count in product, coli-group, pathogenic bacteria all meet national standard: the index really, in vegetables juice beverages health standard (GB19297-2003).Further, place after 18 months and occur without precipitation or research of chaotic phenomenon, and the anthocyanidin content detected wherein is blackberry, blueberry before treatment 96.5%.
Test of pesticide effectiveness example
Animal used as test: Wistar rat 120, male, 180-200g.Rat adapts to nursing after 5 days at Animal House, and randomly draw 10 Normal groups and feed with common maintenance feed, experimental group is fed with high glucose and high fat feed, high glucose and high fat feed formula: rat basal feed 67%, sucrose 11%, yolk powder 9%, lard 8%, cholesterol 1%, cholate 0.2%.
Embodiment: all the other 110 adopt high glucose and high fat forage feed 4 weeks.After 4 weeks, rat is after fasting 18h, Streptozotocin intravenous injection 20mg/kg, and every two days once, and after using twice, the blood pressure and blood lipoid person of remaining high is modeling success.Choose the successful rat of modeling 100, be divided into 5 groups at random, often organize 10, first group is model group, does not namely feed with any medicine or hypoglycemic food; Feed with acarbose hypoglycemic drug for second group, dosage is: 50mg once-a-day 2 days → 50mg, twice on the mono-2 days → 50mg, three times on the mono-3 days → 100mg three times on the mono-, takes before the meal; 3rd group feed with blackberry, blueberry magma, dosage: 15mL/kg once, three times on the one; 4th group feed with blackberry, blueberry sugar-free concentrate, dosage 20mL/kg once, three times on the one; Feed Israel and Palestine for 5th group and take bar extract, the same acarbose of dosage; Feed with Bitter Melon P.E for 6th group, dosage is the same; Feed with blue or green money willow extract for 7th group, dosage is the same; Feed with guava extract for 8th group, dosage is the same; Feed with Radix et Caulis Opuntiae Dillenii extract for 9th group, dosage is the same; The tenth group of composition product containing blackberry, blueberry of feeding with the embodiment of the present invention 1, dosage is with the 3rd group; Take 20 days continuously.
Statistical analysis: data information adopts x ± s, uses SPSS19.0 statistical software to carry out data analysis, compares employing variance analysis between many groups, compares and adopt t inspection between two groups.P<0.05 has statistical significance.
Curative effect: have detected fasting blood-glucose (for FPG), 2h-plasma glucose (2hPG) biochemical indicator, unit is mmol/L.
Group | FPG | 2hPG |
Normal group | 5.26±0.25 | 7.67±0.22 |
Model group | 20.52±3.98 | 26.31±4.01 |
Medicine group | 7.35±0.95 | 8.15±0.86 |
Blackberry, blueberry magma group | 15.28±1.02 | 19.12±1.21 |
Blackberry, blueberry concentrate group | 11.83±0.56 | 14.61±0.62 |
Ba Naba extract | 15.01±3.26 | 23.23±4.13 |
Bitter Melon P.E | 17.94±4.23 | 24.03±3.25 |
Blue or green money willow extract | 18.46±2.01 | 23.65±2.75 |
Guava extract | 16.73±4.56 | 24.37±3.68 |
Radix et Caulis Opuntiae Dillenii extract | 17.83±2.66 | 24.21±3.53 |
Product group of the present invention | 6.73±0.32 | 7.92±0.47 |
Conclusion: on the impact of FPG and 2hPG as seen from the above table, except normal group, model group FPG, 2hPG all obviously raise, comparatively model group is compared, blackberry, blueberry magma group and blackberry, blueberry concentrate group FPG, 2hPG declines all to some extent, but blood sugar concentration is still in higher level, medicine group and product group FPG of the present invention, 2hPG but has obvious decline (P<0.01), product group of the present invention is significantly better than medicine group (P<0.05), also blackberry, blueberry magma group (P<0.01) is better than, blackberry, blueberry concentrate group (P<0.01), Ba Naba extract group (P<0.01), Bitter Melon P.E group (P<0.01), blue or green money willow extract group (P<0.01), guava extract group (P<0.01) and Radix et Caulis Opuntiae Dillenii extract's group (P<0.01).It can thus be appreciated that the blood sugar reducing function of the present invention's combination is not the simple addition of each constituent function, is the synergistic function of each constituent.
Clinical testing example:
Data and method: physical data outpatient service type ii diabetes patient is totally 100 examples, wherein man 59 example, female 41 example, patient age 51-78 years old, fasting blood-glucose (FPG) and 2h-plasma glucose (2hPG) all significantly raise.The hypoglycemic concentrate of blackberry, blueberry sugar-free taking the embodiment of the present invention 1 preparation, after one month, detects above-mentioned biochemical indicator.Cure: FPG<6.1mmol/L, 2h blood sugar (2hPG) <8.0mmol/L.Invalid: fasting blood-glucose (FPG), 2h-plasma glucose (2hPG) are all without obviously changing.Effective: fasting blood-glucose (FPG), 2h-plasma glucose (2hPG) make moderate progress, but do not reach normal level.
Result for the treatment of: cure 71 examples, effective 16 examples, invalid 3 examples, total effective rate is 97%.
Embodiment 2
Formula: blackberry, blueberry 100kg, compound 10kg
Complex components weight:
Take 100kg blackberry, blueberry and freeze fruit, removal of impurities is cleaned after low temperature thaws, add bamboo extractive and the green-tea extract (1:1 of 0.1kg, w/w) color retention is carried out, application beater carries out making beating process, sieve de-seed, its mesh size is between 0.6-0.8mm, to join in blackberry, blueberry magma with continuous 120-140 object seeds that are ground to for three times of colloid mill again, (complex enzyme formulation is the pectase accounting for complex enzyme formulation weight 80% to add 6g/L complex enzyme formulation, 15% cellulase, 4% hemicellulase and 1% proteinase mixture), in 45 DEG C of enzymolysis 50min, in the magma after above-mentioned enzymolysis, adding 1.5g/L invertase trehalose mixed liquor, (invertase trehalose mixed liquor is by invertase, trehalose, pure water mixing composition, part by weight is 4:1:5.), in 50 DEG C of hydrolysis 50min, application Conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, reduce temperature to 35 DEG C and obtain blackberry, blueberry magma, to cooling after blackberry, blueberry magma in inoculate 0.5%(v/v) Lactobacillus plantarum nutrient solution, fully mix immediately, pass into filtrated air, and Keep agitation 4h, now the turbid juice saccharometer of blackberry, blueberry detects the position that scale maintains 0.2 ° substantially, again with conventional ultraviolet and collaborative less than the 85 DEG C sterilization 5-6min of microwave energy integration apparatus, under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrated above-mentioned sterilization to pH3(now cycles of concentration be 5-6 times), its density is suitable, mobility is better, again under agitation, add trehalose successively, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum, (being all after stirring, add lower a kind of material again) at every turn, filling, sealing, last ultraviolet microwave integration apparatus less than 85 DEG C sterilization 5-6min using routine again, under room temperature, be cooled to normal temperature obtain.Inspection: after sterilization cooling, by country fruit, vegetables juice beverages health standard (GB19297-2003), carries out sense organ and physics and chemistry, bioassay to blackberry, blueberry sugar-free concentrate product immediately, rejects and leak tank, be up to the standards, and coding vanning list marketing, the shelf-life is 18 months.
Through inspection, the content of arsenic, lead, copper and total plate count in product, coli-group, pathogenic bacteria all meet national standard: the index really, in vegetables juice beverages health standard (GB19297-2003).Further, place after 18 months and occur without precipitation or research of chaotic phenomenon, the anthocyanidin content detected wherein is blackberry, blueberry before treatment 95.7%.
Claims (7)
1. there is the composition containing blackberry, blueberry of function of reducing blood sugar, it is characterized in that said composition is made primarily of following raw material:
The mass ratio 20:1-2 of blackberry, blueberry and compound; Wherein, compound according to each component of weight percent content is: Ba Naba extract 20%-35%, Bitter Melon P.E 20%-35%, blue or green money willow extract 15%-30%, guava extract 8%-25%, Radix et Caulis Opuntiae Dillenii extract 5%-25%, Arabinose 3%-10%, trehalose 2%-10%, Hericium erinaceus extract 5%-20%, Arabic gum 1 ‰-2.5 ‰;
Said composition is prepared by following steps:
A), the process of blackberry, blueberry raw material: get blackberry, blueberry, add the bamboo extractive of blackberry, blueberry quality 1 ‰-3 ‰ and the mixture of green-tea extract, the weight ratio of bamboo extractive and green-tea extract is 1:1, after mixing, making beating, sieve de-seed, and the seed taken off is ground to 120-140 orders, by its add-back blackberry, blueberry magma; Add the complex enzyme formulation of 6g/L again, enzymolysis 40-60min at 40 DEG C-50 DEG C;
B), the sugar-free preparatory stage: the invertase trehalose mixed liquor adding 1.5-5g/L in the magma after above-mentioned enzymolysis, is hydrolyzed 40-60min at 40-50 DEG C, and go out enzyme and sterilizing 5-6min; Wherein, invertase trehalose mixed liquor is made up of invertase, trehalose, pure water, and part by weight is 4:1:5;
C), sugar-free operation stage: reduce temperature to 30-35 DEG C, inoculate the Lactobacillus plantarum nutrient solution of 0.5%-1% in above-mentioned blackberry, blueberry magma, fully mix, pass into filtrated air, and Keep agitation 4-10h, detect between 0 °-0.3 ° to the turbid juice pol of blackberry, blueberry, then sterilization 5-6min;
D) it is, concentrated filling: under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrating sterilizing is to pH2-3, under agitation, add trehalose, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum successively, sterile filling, sealing, sterilization 5-6min, is cooled to normal temperature.
2. the composition containing blackberry, blueberry with function of reducing blood sugar according to claim 1, is characterized in that said composition is made primarily of following raw material:
The mass ratio 20:1 of the compound of blackberry, blueberry and plant extracts; Wherein, the compound of plant extracts according to each component of weight percent content is: Ba Naba extract 25%-30%, Bitter Melon P.E 25%-30%, blue or green money willow extract 15%-25%, guava extract 8%-15%, Radix et Caulis Opuntiae Dillenii extract 8%-15%, Arabinose 3%-5%, trehalose 2%-5%, Hericium erinaceus extract 5%-10%, Arabic gum 1 ‰-2 ‰.
3. the composition containing blackberry, blueberry with function of reducing blood sugar according to claim 1, is characterized in that the product form of described composition is concentrate, fruit juice, jam or dry powder.
4. the preparation method with the composition containing blackberry, blueberry of function of reducing blood sugar described in claim 1 or 2, is characterized in that the method comprises the following steps:
A), the process of blackberry, blueberry raw material: get blackberry, blueberry, add the bamboo extractive of blackberry, blueberry quality 1 ‰-3 ‰ and the mixture of green-tea extract, the weight ratio of bamboo extractive and green-tea extract is 1:1, after mixing, making beating, sieve de-seed, and the seed taken off is ground to 120-140 orders, by its add-back blackberry, blueberry magma; Add the complex enzyme formulation of 6g/L again, enzymolysis 40-60min at 40 DEG C-50 DEG C;
B), the sugar-free preparatory stage: the invertase trehalose mixed liquor adding 1.5-5g/L in the magma after above-mentioned enzymolysis, is hydrolyzed 40-60min at 40-50 DEG C, and go out enzyme and sterilizing 5-6min; Wherein, invertase trehalose mixed liquor is made up of invertase, trehalose, pure water, and part by weight is 4:1:5;
C), sugar-free operation stage: reduce temperature to 30-35 DEG C, inoculate the Lactobacillus plantarum nutrient solution of 0.5%-1% in above-mentioned blackberry, blueberry magma, fully mix, pass into filtrated air, and Keep agitation 4-10h, detect between 0 °-0.3 ° to the turbid juice pol of blackberry, blueberry, then sterilization 5-6min;
D) it is, concentrated filling: under temperature is 40-45 DEG C of conditions, blackberry, blueberry magma after concentrating sterilizing is to pH2-3, under agitation, add trehalose, Arabinose, Ba Naba extract, Bitter Melon P.E, blue or green money willow extract, guava extract, Radix et Caulis Opuntiae Dillenii extract, Hericium erinaceus extract, Arabic gum successively, sterile filling, sealing, sterilization 5-6min, is cooled to normal temperature.
5. the preparation method of composition containing blackberry, blueberry with function of reducing blood sugar according to claim 4, is characterized in that described complex enzyme formulation is account for the pectase of complex enzyme formulation weight 80%, 15% cellulase, 4% hemicellulase and 1% protease.
6. the preparation method of composition containing blackberry, blueberry with function of reducing blood sugar according to claim 4, is characterized in that described step b) in go out enzyme and sterilizing and step c) and d) in sterilization temperature all not higher than 85 DEG C.
7. the preparation method with the composition containing blackberry, blueberry of effect of lowering blood sugar according to claim 4, when it is characterized in that compound adds, often adds after a kind of component mixes and adds lower a kind of material again.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310752392.2A CN103750102B (en) | 2013-12-31 | 2013-12-31 | There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310752392.2A CN103750102B (en) | 2013-12-31 | 2013-12-31 | There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103750102A CN103750102A (en) | 2014-04-30 |
CN103750102B true CN103750102B (en) | 2016-03-09 |
Family
ID=50517556
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310752392.2A Active CN103750102B (en) | 2013-12-31 | 2013-12-31 | There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103750102B (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TW201634056A (en) * | 2015-03-25 | 2016-10-01 | bo-zheng Wang | Dietary composition of guava fruit with trehalose for improvement of type ii diabetes and use thereof |
CN104996857A (en) * | 2015-06-30 | 2015-10-28 | 安徽鸿泰食品有限公司 | Fermented blackberry jam |
CN105919076A (en) * | 2016-04-26 | 2016-09-07 | 青岛金凤凰庄园果品有限公司 | Fermentation method of blackberry single ferment |
CN106237315A (en) * | 2016-08-02 | 2016-12-21 | 马南行 | A kind of compositions of external curing diabetes and preparation method thereof |
CN107184464B (en) * | 2017-05-25 | 2018-01-23 | 上海悦目化妆品有限公司 | A kind of Compound Black plant fermentation composition with white-skinned face function and preparation method thereof and the application in facial mask class product |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101133771A (en) * | 2007-10-10 | 2008-03-05 | 辽宁今日农业有限公司 | Method of producing bramble leaf tea beverage |
CN102630787A (en) * | 2012-04-16 | 2012-08-15 | 张明玉 | Composite raspberry fruit tea and processing method thereof |
-
2013
- 2013-12-31 CN CN201310752392.2A patent/CN103750102B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101133771A (en) * | 2007-10-10 | 2008-03-05 | 辽宁今日农业有限公司 | Method of producing bramble leaf tea beverage |
CN102630787A (en) * | 2012-04-16 | 2012-08-15 | 张明玉 | Composite raspberry fruit tea and processing method thereof |
Non-Patent Citations (2)
Title |
---|
降糖功能因子天然资源的分布概况;陈丽华等;《现代食品科技》;20051231;第21卷(第3期);172-175 * |
食用菌的降血糖作用研究进展;芦菲等;《食品研究与开发》;20100228;第31卷(第2期);189-192 * |
Also Published As
Publication number | Publication date |
---|---|
CN103750102A (en) | 2014-04-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103750102B (en) | There is the composition and method of making the same containing blackberry, blueberry of function of reducing blood sugar | |
CN104872647A (en) | Enzyme containing medicinal and edible homologous plants and preparation method of enzyme | |
EP3185877B1 (en) | Process for enhancing the viable counts of lactic acid bacteria and useful compositions thereof | |
CN105815641A (en) | Preparation method of comprehensive yeast liquid with anti-tumor activity and application of comprehensive yeast liquid | |
CN106579387A (en) | Arabinose-enzyme composition and preparation method thereof | |
CN105211864A (en) | A kind of black fruit lyceum mixed fruit ferment and preparation method thereof | |
CN105211867A (en) | A kind of black fruit lyceum compound fruit ferment and preparation method thereof | |
CN110200151A (en) | A kind of yak feed addictive and preparation method thereof that yak survival rate can be improved | |
CN110037287A (en) | A kind of mulberry enzyme powder and preparation method thereof | |
CN107594109A (en) | A kind of pet drinks enzyme liquid and preparation method thereof | |
CN104000192B (en) | A kind of functional food and two-step fermentation preparation method thereof releasing the pressure and regulate stomach | |
CN107173656A (en) | A kind of Chinese medicine fruits and vegetables probiotics fermented beverage and preparation method thereof | |
CN106858577A (en) | Prepare the fermentation composition and preparation method of the plant enzyme with liver protecting, the fatty subtotal hepatectomy of regulation | |
CN103932181B (en) | A kind ofly to release the pressure and the functional food of Improving memory and two-step fermentation preparation method thereof | |
CN102835453A (en) | Making method of vegetable milk | |
CN103948023A (en) | Health foods for enhancing immunity and improving sleep and two-step fermentation preparation method thereof | |
CN103932180B (en) | A kind ofly to release the pressure and the health food of antifatigue and two-step fermentation preparation method thereof | |
CN109043553A (en) | A kind of Special food and preparation method thereof that patients with gestational diabetes is edible | |
CN104856016A (en) | Highland barley monascus purpureus enzyme and preparation method thereof | |
CN103932179B (en) | A kind of health food and two-step fermentation preparation method thereof releasing the pressure and protect liver | |
CN107927366A (en) | New disease-resistant lamb supplement material of height and preparation method thereof | |
CN107896921A (en) | The white fungus bacterium bioconversion composition and its manufacture method of maca and RHIIZOMA DIOSCOREAE from Henan of China | |
KR101925124B1 (en) | Manufacturing method for fermented drink using vegetable sourse | |
CN105233274A (en) | Oral composition containing cistanche and enzymes | |
KR101252249B1 (en) | Fermented composition using effective micro-organisms and manufacturing method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant |