CN106047723A - Preparation of phellinus linteus mycelia by taking red dates and wolfberry fruits as culture medium - Google Patents
Preparation of phellinus linteus mycelia by taking red dates and wolfberry fruits as culture medium Download PDFInfo
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- CN106047723A CN106047723A CN201610480554.5A CN201610480554A CN106047723A CN 106047723 A CN106047723 A CN 106047723A CN 201610480554 A CN201610480554 A CN 201610480554A CN 106047723 A CN106047723 A CN 106047723A
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- culture medium
- mycelia
- fructus
- fructus jujubae
- fructus lycii
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention provides preparation of phellinus linteus mycelia by taking red dates and wolfberry fruits as a culture medium. The red dates and the wolfberry fruits are used as main raw materials of a culture medium; after the culture medium is sterilized, phellinus linteus strains are inoculated and then are cultured until hyphae grow all over the culture medium, so as to obtain red date, wolfberry fruit and phellinus linteus combined hypha blocks; and finally, the mycelium blocks are solarized or dried and then are crushed into the mycelia. With the implementation of the preparation, the red dates, the wolfberry fruits and the phellinus linteus are combined through a biotechnology to form novel special red date, wolfberry fruit and phellinus linteus mycelia; the formation of the product is special and novel and nutrients of the product are enriched better; and functions and health effect of the product are relatively prominent. The phellinus linteus mycelia are of a novel and special health product. Meanwhile, a production process of the red date, wolfberry fruit and phellinus linteus combined hypha blocks has the characteristics of short culture period, simple production and preparation process, high and stable production efficiency of the product and the like.
Description
Technical field
The invention belongs to biological technical field, be specifically related to one and prepare phellinus liteus with Fructus Jujubae and Fructus Lycii for culture medium
Body.
Background technology
Phellinus igniarius (L. ex Fr.) Quel. is rare medicinal fungi, and its sporophore can be used as medicine.Its main parasitic is in broad leaf trees such as Mulberry, poplar, willow, birch, Fructus Persicae
Dry wood and standing tree on and trunk on, its major function is anticancer, fibrosis, antioxidation, its treatment cancer effective percentage
Reach more than 90%.Phellinus igniarius (L. ex Fr.) Quel. in biological anticancer field by internationally recognized be the fungus of most anticancer function, it has in anti-cancer field
Efficiency ranks the first, and is international the world of medicine and health products trade antineoplastic product raw materials for production.The Chinese medicine that this taste is ancient, reputation is the most not
Such as Cordyceps, but its effect is but poor unlike Cordyceps.At present, internationally recognized biological phellin polysaccharides is from Phellinus igniarius (L. ex Fr.) Quel.
Mycelium, sporophore extract there is bioactive polysaccharose substance.Research confirms, phellin polysaccharides has substantially immunity and adjusts
Joint, anticancer antitumor, antioxidation, reduction blood glucose, the liver protecting etc. act on.
And the factor that restricting current the further development and utilization of Phellinus igniarius (L. ex Fr.) Quel. mainly has: (1) Phellinus igniarius (L. ex Fr.) Quel. wild resource is the deficientest,
Wild Phellinus igniarius (L. ex Fr.) Quel. resource is difficult to recover, and causes Phellinus igniarius (L. ex Fr.) Quel. sporophore to be formed in a large number；(2) Phellinus igniarius (L. ex Fr.) Quel. is environmentally sensitive, and trophophase is long, people
Work cultivation difficulty is bigger.In order to solve because the deficiency of wild Phellinus igniarius (L. ex Fr.) Quel. resource and artificial culture difficulty cause greatly Phellinus igniarius (L. ex Fr.) Quel. sporophore
Output cannot then affect the application problem in Phellinus igniarius (L. ex Fr.) Quel. medicine source in a large number；Constantly study through scientific research personnel, deep now by biology
Layer fermentation technique develops brand-new a kind of Phellinus igniarius (L. ex Fr.) Quel. product phellinus igniarius mycelium.Research shows, effective one-tenth of phellinus igniarius mycelium
Divide the same with sporophore.Producing phellinus igniarius mycelium by biological submerged fermentation technology, it is with short production cycle, technique is simple, production
The features such as efficiency is high and stable, are the main development directions of artificial culture Phellinus igniarius (L. ex Fr.) Quel. product.Produce at present used by phellinus igniarius mycelium
Raw material is mainly grain concentrate, and such as Semen Maydis powder, analysis for soybean powder etc., adding a little inorganic salt element on the basis of this raw material is carbon and nitrogen sources,
Then the phellinus igniarius mycelium of high-quality low-cost is turned out.
Learning from relevant report, extract or leachate with Phellinus igniarius (L. ex Fr.) Quel. sporophore are processed into beverage, food or medicine.Also
Develop various Phellinus igniarius (L. ex Fr.) Quel. sporophore and become ultra-micro powder capsule class Orally taken product, Phellinus igniarius (L. ex Fr.) Quel. artificial hyphostoma's culture through breaking cellular wall post-treatment
Deng.
Summary of the invention
It is an object of the invention to provide brand-new one and prepare phellinus igniarius mycelium with Fructus Jujubae and Fructus Lycii for culture medium.Specifically
Including: this mycelium be with Fructus Jujubae, Fructus Lycii be primary raw material as culture medium, by accessing Phellinus igniarius (L. ex Fr.) Quel. strain after medium sterilization, connect
Cultivation to mycelia to cover with culture medium and obtain Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelia block, finally by mycelia block through shining or drying
After be ground into mycelium.
Described Fructus Jujubae, has another name called Fructus Jujubae.Feature is that vitamin content is the highest, has the good reputation of " natural complex ball ", tool
There is nourishing YIN and invigorating YANG, the effect enriched blood.Fructus Jujubae is a kind of nutrient excellent product, is described as " king of BAIGUO ".It is raw that Fructus Jujubae contains abundant dimension
The necessary multivitamin of human body and 18 kinds of aminoacid, mineral such as element A, B, C, the wherein content of vitamin C (ascorbic acid)
Unexpectedly be up to Fructus Vitis viniferae, 70～80 times of Fructus Mali pumilae, the content of rutin (Citrin) is the highest, both vitamin to anti-cancer and prevention
Hypertension, hyperlipidemia have certain effect.Fructus Jujubae sweet in the mouth warm in nature, containing protein, fat, sugar, calcium, phosphorus, ferrum, magnesium and abundant
Vitamin A, vitamin C, vitamin B1, vitamin B2, additionally contain carotene etc., and nutrition is the abundantest, among the people have " my god
It eats Fructus Jujubae, and all one's life bears one's age well " say.The fruit that people like is not only by Fructus Jujubae, be also simply nourishing spleen and stomach, nourishing blood to tranquillize the mind,
The good medicine promoted health and cured diseases.Puerpera eats Fructus Jujubae, energy invigorating the spleen and replenishing QI, nourishing blood to tranquillize the mind, accelerates body and restores；Oldaged physically weak person eats
Fructus Jujubae, energy health invigorating, slow down aging；The people being more especially engaged in mental work and neurastheniac, boil soup generation with Fructus Jujubae
Tea, can feel at ease to keep god, appetite stimulator.Have the people of tea addiction, cross drink night, toss about in bed sleeplessness unavoidably, if decocting soup generation with Fructus Jujubae every night
Tea, can exempt the hardship of insomnia.In season in spring and autumn, first tremble with fear and first warm up, Fructus Jujubae adds several Folium Mori and decocts Tang Daicha, cold sense can be prevented
Emit；Summertime is hot, and Fructus Jujubae and Folium Nelumbinis are with boiling and can relieve summer heat by promoting the circulation of QI；Severe cold of winter, Fructus Jujubae Tonga fresh ginger brown sugar, warming the stomach of can dispeling cold.Red
Fructus Jujubae is natural beauty food, also can replenishing QI to invigorate the spleen, promote QI and blood biochemistry circulation and defying age.Fructus Jujubae is not only a kind of deep by old
The food that the common people like, is also the Chinese medicine commonly used simply.Modern pharmacological research finds, containing more protein, ammonia in Fructus Jujubae
Base acid, saccharide, organic acid, vitamin A, vitamin B2. vitamin C, P etc., and microelements of calcium, phosphorus, potassium, ferrum, magnesium,
Aluminum and substantial amounts of cyclic adenosine monophosphate etc..Its warm in nature, sweet in the mouth, has invigorating the spleen and replenishing QI, nourishing blood to tranquillize the mind, born fluid, solution poison of drug, obtundent
Effect of property.The method that " the nourishing the liver soup " of the traditional Chinese medical science the most useful Fructus Jujubae prescription carrys out liver-nourishing, toxin-expelling.Hubei Provincial Hospital of TCM state of internal organs liver
Sick director of the Institute doctoral advisor professor Sheng Guoguang introduces, Fructus Jujubae have protect the liver, spleen invigorating, reduction cholesterol, raise leukocyte,
Antiallergic etc. act on.
Described Fructus Lycii is that people are to the system of species under the Lyciums such as commodity Fructus Lycii, plant lycium barbarum, China Fructus Lycii
Claim (Chinese medicine claims Fructus Lycii).With fine jade treasure Ganoderma, Changbai Mountain Radix Ginseng, Donga Colla Corii Asini referred to as Chinese medicine four are precious.The Main Ingredients and Appearance of Fructus Lycii:
Lycium barbarum polysaccharide, Fructus Lycii contain glycine betaine (betane), atropine (atropine), hyoscyamine (gyoscyamine), Fructus Lycii color
Element etc..Wherein, lycium barbarum polysaccharide is a kind of water soluble polysaccharide, is topmost active component in Fructus Lycii, and relative molecular mass is 68-
200, become domestic and international study hotspot.The most again with immunomodulating and the most study of antitumor action of lycium barbarum polysaccharide.?
Lycium barbarum polysaccharide has promotion immunity, defying age, antitumor, removing free radical, resisting fatigue, radioprotective, guarantor to have a lot of research to show
The effects such as liver, reproductive function protection and improvement.Glycine betaine, chemical name is 1-carboxy-N, N, N-betaine, is changing
With amino acid similarity in structure, belong to quaternary amine alkaloids.Glycine betaine is one of alkaloid main in FRUCTUS LYCII, leaf, handle.
Lipid metabolism or lipotropic effect are caused by Fructus Lycii mainly due to contained glycine betaine, and it plays methyl supply in vivo
The effect of body.Research about Fructus Lycii glycine betaine is only limitted to the domestic mensuration at content, extraction process and the life to lycium plant
Reason effect (enhancing salt tolerance) research aspect, the pharmacological effect about Fructus Lycii glycine betaine is studied seldom.Wolfberry pigment is present in
All kinds of present-color materials in Fructus Lycii berry, are the important physiologically active ingredients of seed of Fructus Lycii.Mainly include-carotene, phylloxanthin and
Other coloring matters.Carotenoid contained by Fructus Lycii then has very important medical value.A lot of researchs have turned out Chinese holly
Qi seed pigment has raising immune function of human body, prevent and suppress tumor and prevention of arterial is atherosis etc. acts on.Carotene is
The main active of wolfberry pigment, has antioxidation and the synthesis important physiological function such as precursor as vitamin A.
The present invention is achieved through the following technical solutions:
Prepare phellinus igniarius mycelium with Fructus Jujubae and Fructus Lycii for culture medium, technical scheme is: this mycelium is to be with Fructus Jujubae, Fructus Lycii
Primary raw material, as culture medium, by accessing Phellinus igniarius (L. ex Fr.) Quel. strain after medium sterilization, is then cultivated and is covered with culture medium to mycelia and obtain
Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelia block, be finally ground into mycelium by mycelia block after shining or drying.
Specifically include following steps:
1, Fructus Jujubae collection and process: gather and parch, without rotten, the normal red date as raw materials of color and luster, then by extracting red date stone and break
It is broken into as niblet size standby.
2, Fructus Lycii gathers and processes: collection parches, is raw material without rotten, the normal Fructus Lycii of color and luster.
3, preparation culture medium: culture medium is the rice meal group of the Fructus Jujubae by 48%, the Fructus Lycii of 40%, the Semen Maydis powder of 7% and 5%
Becoming, first mix above-mentioned various raw materials stir to obtain compound, then admixes in compound with the brown sugar water of 3%,
The moisture content of compound is adjusted to 55-60%, then mixed material is sub-packed in culture vessel, and charge weight is that mixed material exists
Thickness in culture vessel is 2-3cm, the most conventionally must produce after sterilizing and use culture medium.
4, inoculation: access Phellinus igniarius (L. ex Fr.) Quel. strain in an aseptic environment.
5, cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, relatively
Humidity is to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
6, cultivate mycelia block: when phellinus liteus covers with culture medium, continue to cultivate Fructus Jujubae and Chinese holly intertexture connection to mycelia
Become mycelia block.
7, dry: through shining or drying to material moisture less than 8% in the environment of mycelia block is placed below 50 °.
8, finished product: the mycelia block through shining or dry is crushed to cross 100-120 mesh and obtains finished product mycelium.
The present invention both had the following advantages:
1, the proposed by the invention a kind of mycelium its preparation method combined by Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. is novel, raw
Production. art is simply, easily implement.
2, by implementing the present invention, by biotechnology Fructus Jujubae, Fructus Lycii, the combination of Phellinus igniarius (L. ex Fr.) Quel. combination another characteristic of formation
Body Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelium, the formation of product not only has characteristic, novelty, and makes its nutrition richer
Collection, and its function and health-care effect are more prominent.It is the health product of a kind of novel characteristic.
3, by implementing the present invention, final products Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combine mycelial process for producing tool
There are the features such as cultivation period is short, process for producing simple, the production efficiency of product is high and stablizes.
4, by implementing the present invention, the product purpose of formation is extensive, can be as being prepared as medicine, health product, characteristic food
The raw material of product additive.Also the health product obtaining a kind of characteristic can be loaded in capsule.Not only there is extraordinary market prospect, and
The economic benefit produced also can highly significant.
Detailed description of the invention
Below in conjunction with embodiment, the method for the present invention is further illustrated.
Prepare phellinus igniarius mycelium with Fructus Jujubae and Fructus Lycii for culture medium, technical scheme is: this mycelium is with Fructus Jujubae, Chinese holly
Qi is primary raw material as culture medium, will access Phellinus igniarius (L. ex Fr.) Quel. strain after medium sterilization, then cultivate to mycelia cover with culture medium and
Obtain Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelia block, finally mycelia block is ground into mycelium after shining or drying.
Embodiment 1:
1, Fructus Jujubae collection and process: gather and parch, without rotten, the normal red date as raw materials of color and luster, then by extracting red date stone and break
It is broken into as niblet size standby.
2, Fructus Lycii gathers and processes: collection parches, is raw material without rotten, the normal Fructus Lycii of color and luster.
3, preparation culture medium: culture medium is the rice meal group of the Fructus Jujubae by 48%, the Fructus Lycii of 40%, the Semen Maydis powder of 7% and 5%
Becoming, first mix above-mentioned various raw materials stir to obtain compound, then admixes in compound with the brown sugar water of 3%,
The moisture content of compound is adjusted to 55-60%, then mixed material is sub-packed in culture vessel, and charge weight is that mixed material exists
Thickness in culture vessel is 2-3cm, the most conventionally must produce after sterilizing and use culture medium.
4, inoculation: access the Phellinus igniarius (L. ex Fr.) Quel. strain of 7-8 point in an aseptic environment.
5, cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, relatively
Humidity is to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
6, cultivate mycelia block: when phellinus liteus covers with culture medium, continue to cultivate Fructus Jujubae and Chinese holly intertexture connection to mycelia
Become mycelia block.
7, dry: through shining or drying to material moisture less than 8% in the environment of mycelia block is placed below 50 °.
8, finished product: the mycelia block through shining or dry is crushed to cross 100-120 mesh and obtains finished product mycelium.
9, commodity: mycelium is dispensed in capsule to obtain a kind of Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelium capsule.
Embodiment 2:
1, Fructus Jujubae collection and process: gather and parch, without rotten, the normal red date as raw materials of color and luster, then by extracting red date stone and break
It is broken into as niblet size standby.
2, Fructus Lycii gathers and processes: collection parches, is raw material without rotten, the normal Fructus Lycii of color and luster.
3, preparation culture medium: culture medium is the rice meal group of the Fructus Jujubae by 48%, the Fructus Lycii of 40%, the Semen Maydis powder of 7% and 5%
Becoming, first mix above-mentioned various raw materials stir to obtain compound, then admixes in compound with the brown sugar water of 3%,
The moisture content of compound is adjusted to 55-60%, then mixed material is sub-packed in culture vessel, and charge weight is that mixed material exists
Thickness in culture vessel is 2-3cm, the most conventionally must produce after sterilizing and use culture medium.
4, inoculation: access 10～the Phellinus igniarius (L. ex Fr.) Quel. strain of 12% according to the weight of culture medium in an aseptic environment.
5, cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, relatively
Humidity is to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
6, cultivate mycelia block: when phellinus liteus covers with culture medium, continue to cultivate Fructus Jujubae and Chinese holly intertexture connection to mycelia
Become mycelia block.
7, dry: through shining or drying to material moisture less than 8% in the environment of mycelia block is placed below 50 °.
8, finished product: the mycelia block through shining or dry is crushed to cross 100-120 mesh and obtains finished product mycelium.
9, commodity: mycelium is dispensed in capsule to obtain a kind of Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelium capsule.
Claims (1)
1. prepare phellinus igniarius mycelium with Fructus Jujubae and Fructus Lycii for culture medium, it is characterised in that: this mycelium is to be main with Fructus Jujubae, Fructus Lycii
Want raw material as culture medium, by accessing Phellinus igniarius (L. ex Fr.) Quel. strain after medium sterilization, then cultivate and cover with culture medium to mycelia and obtain red
Fructus Jujubae, Fructus Lycii, Phellinus igniarius (L. ex Fr.) Quel. combination mycelia block, be finally ground into mycelium by mycelia block after shining or drying；
Specifically include following steps:
(1) Fructus Jujubae collection and process: gather and parch, without rotten, the normal red date as raw materials of color and luster, then by extracting red date stone and break
It is broken into as niblet size standby；
(2) Fructus Lycii gathers and processes: collection parches, is raw material without rotten, the normal Fructus Lycii of color and luster；
(3) culture medium is prepared: culture medium is made up of the rice meal of Fructus Jujubae, the Fructus Lycii of 40%, the Semen Maydis powder of 7% and 5% of 48%, first
Mix above-mentioned various raw materials stir to obtain compound, then admixes in compound with the brown sugar water of 3%, mixing
The moisture content of material is adjusted to 55-60%, then mixed material is sub-packed in culture vessel, and charge weight is that mixed material is being cultivated
Thickness in container is 2-3cm, the most conventionally must produce after sterilizing and use culture medium；
(4) inoculation: access Phellinus igniarius (L. ex Fr.) Quel. strain in an aseptic environment；
(5) cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, the wettest
Degree is to cultivate in the environment of 60～65% to mycelia to cover with culture medium；
(6) cultivate mycelia block: when phellinus liteus covers with culture medium, continue cultivation, to mycelia, Fructus Jujubae and Chinese holly intertexture are connected into bacterium
Silk block；
(7) dry: through shining or drying to material moisture less than 8% in the environment of mycelia block is placed below 50 °；
(8) finished product: the mycelia block through shining or dry is crushed to cross 100-120 mesh and obtains finished product mycelium.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041585A (en) * | 2017-12-29 | 2018-05-18 | 宁夏回族自治区农业学校 | A kind of preparation method of Phellinus nutritional meal replacement powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583236A (en) * | 2013-11-16 | 2014-02-19 | 覃秀花 | Method for preparing fleckedflesh polypore mycelium |
| CN105558735A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Edible fungus mycelium fermented drink and making method thereof |
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2016
- 2016-06-28 CN CN201610480554.5A patent/CN106047723A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583236A (en) * | 2013-11-16 | 2014-02-19 | 覃秀花 | Method for preparing fleckedflesh polypore mycelium |
| CN105558735A (en) * | 2016-01-25 | 2016-05-11 | 四川保兴现代农业科技股份有限公司 | Edible fungus mycelium fermented drink and making method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041585A (en) * | 2017-12-29 | 2018-05-18 | 宁夏回族自治区农业学校 | A kind of preparation method of Phellinus nutritional meal replacement powder |
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