CN107095300A - A kind of biological nutrition compound composition of auxiliary treatment diabetes - Google Patents

A kind of biological nutrition compound composition of auxiliary treatment diabetes Download PDF

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CN107095300A
CN107095300A CN201710134651.3A CN201710134651A CN107095300A CN 107095300 A CN107095300 A CN 107095300A CN 201710134651 A CN201710134651 A CN 201710134651A CN 107095300 A CN107095300 A CN 107095300A
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extract
powder
parts
chromium
treatment diabetes
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王鹏
高峰
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Liaoning Shanshui Plant Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/73Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00Medicinal preparations containing inorganic active ingredients
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/07Basidiomycota, e.g. Cryptococcus
    • A61K36/074Ganoderma
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/285Aucklandia
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Abstract

A kind of biological nutrition compound composition of auxiliary treatment diabetes, is prepared from by the raw material of following parts by weight:Black 50 70 parts of fruit Sorbus alnifloria extract;Herba Saussureae Involueratae extract 15 25 parts of powder of original;15 25 parts of Ganoderma rich in chromium mycelium powder;Above raw material amounts to 100 parts.The present invention can improve insulin sensitivity, and softening and dredging blood vessel improves liver and renal glucose sugar generation and storage capacity, maintains carbohydate metabolism balance, while reducing or offsetting the side effect of traditional treatment diabetes.

Description

A kind of biological nutrition compound composition of auxiliary treatment diabetes
Technical field
The present invention relates to technical field of nutritional food, and in particular to a kind of biological nutrition formula group of auxiliary treatment diabetes Compound.
Background technology
Diabetes are the metabolic diseases occurred by the Other Risk Factors such as heredity, environment, behavior collective effect, are also tight One of four major class chronic diseases of China's residents ' health are threatened again.Wound of the diabetes except causing body and mind to patient and its friends and family Evil, also brings huge economic loss.China puts into (25,000,000,000 dollars) of nearly 173,400,000,000 RMB every year is used for diabetes management; Direct medical expense for diabetes accounts for the 13% of Chinese medical expenditure.
The World Health Organization (WHO) gave a warning the World Hygiene Day in 2016:China there are about 1.1 hundred million patient of diabetes Person, if not taking action as early as possible, it is contemplated that the numeral will increase to 1.5 hundred million people in the year two thousand forty.
Type 1 diabetes are also referred to as adolescent diabetes, be mainly characterized in that in patient's body can not generate enough insulin, it is necessary to Daily use of exogenous insulin;Diabetes B is common in the elderly, is that body can not be caused effectively using insulin, accounts for Diabetic's number accounts for the 90% of patient populations.The diabetes patient that the course of disease is longer, control is poor, be often accompanied by various complication or With disease.
The diabetes B illness rate of China was in explosive growth in past more than 20 years, and it is not main reason is that Healthy lifestyles, the dietary structure and shortage of such as high sugared and high fat are moved, and cause Chinese overweight and fat ratio increasingly Rise.Diabetes and diabetic complication cause nearly 1,000,000 people dead every year;It is a concern that wherein nearly 40% death For premature death (below 70 years old in population).China's diabetic's number is numerous, prevention and control situation very severe.
In the management of diabetes, melbine is recommended in the diabetes guide that many countries and international organization formulate at present As the basic medication in the fiest-tire medication and drug combination of diabetes B Patients' rights hyperglycaemia, Body weight loss can be made, reduced Type 2 Diabetes Mellitus Patients with Obesity cardiovascular event and death.
The major side effects of melbine are gastrointestinal reaction.Gastrointestinal symptom sees 10% patient, can have abdominal discomfort, Apocleisis, nausea, diarrhoea, occasionally there is dry or metallic taste, and also only a few people can induce lactic acidosis.
Also sulfonylureas and benzoic acid derivative class promote to secrete agent, if improper use can cause hypoglycemia, particularly exist The infull person of gerontal patient and Liver and kidney function;Sulfonylurea drugs may further result in increased weight.
For OHA failure or partial failure patient it is necessary to be continuing with oral hypoglycemic drug, and imitate in injecting Or protamine zine insulin.Short-acting insulin can be used for the severe diabetes such as drip-feed treatment DKA;Type 1 diabetes once Diagnosis, still needs to lifelong subcutaneous insulin and controls;Diabetes B can use insulin complement treatment or replacement therapy.Such medicine can be sent out Wave blood sugar reducing function, and significantly reduce body weight, enhancing insulin secretion, glucagon suppression secrete and can delay gastric emptying, Food-intake is reduced with the appetite-suppressing by central.But side effect has hypoglycemic reaction and gastrointestinal side effect.
From the point of view of the method for the treatment of diabetes at present, effect is really good, but side effect is really many, using not only effectively but also without pair The naturopathy of effect, aids in or substitutes traditional medicine and insulin therapy, it has also become the craving of numerous diabetics.
The content of the invention
For defect of the prior art, the present invention provides a kind of biological nutrition formula combination of auxiliary treatment diabetes Thing.
A kind of biological nutrition compound composition of auxiliary treatment diabetes, is prepared from by the raw material of following parts by weight:
Black 50-70 parts of fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract 15-25 parts of powder of original;
15-25 parts of Ganoderma rich in chromium mycelium powder;
Above raw material amounts to 100 parts.
In the present invention, as a kind of perferred technical scheme, a kind of biological nutrition formula combination of auxiliary treatment diabetes Thing, is prepared from by the raw material of following parts by weight:
Black 60 parts of fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract 20 parts of powder of original;
20 parts of Ganoderma rich in chromium mycelium powder.
In the present invention, as a kind of perferred technical scheme, the content of chromium is 0.1- in the composition 0.05wt%.Chromium is trivalent chromium, the edible Organic Chromium of state approval.
In the present invention, as a kind of perferred technical scheme, the composition is prepared using following methods:
Weigh the former powder of black fruit Sorbus alnifloria extract, Herba Saussureae Involueratae extract, Ganoderma rich in chromium mycelium powder, tabletting, packaging.
Such as during tabletting, those skilled in the art can also can as needed add necessary auxiliary material with direct tablet compressing, micro- Crystalline cellulose etc..
Tabletting is 0.5g/, daily 6 when taking, chews or swallows.Infant, children and fungi polysaccharide allergy sufferers are not Preferably take.
In the present invention, as a kind of perferred technical scheme, the black fruit Sorbus alnifloria extract is prepared using following methods:Take The fruit of black fruit Sorbus alnifloria is crushed, and is 1 according to solid-liquid ratio:5-10 adds distilled water, is warming up to 50-65 DEG C, is kept for 3-5 minutes, so It is 1 to add solid-liquid ratio afterwards:3-5 ethanol solution, adjustment pH is 3.5-4.5, and in the case where temperature is 32-35 DEG C, heating water bath extracts 2- 3 hours, extract solution is then centrifuged into 30-45min under 2000-2800 revs/min of speed, and be warming up to 45-48 DEG C of decompression Concentration, is then spray-dried, obtains black fruit Sorbus alnifloria extract.
In the present invention, as a kind of perferred technical scheme, the volume ratio of ethanol and water is 1 in ethanol solution:1.
In the present invention, as a kind of perferred technical scheme, the preparation method of Herba Saussureae Involueratae extract original powder is as follows:By day Mountain saussurea involucrata tissue culture is dried 8-10 hours using 70-80 DEG C of cryogenic dewatering of oven, obtains below water content 8wt% Herba Saussureae Involueratae tissue culture dry powder, Herba Saussureae Involueratae tissue culture dry powder is crushed to 80 mesh, 80wt% ethanol is utilized Solution low temperature is extracted, and feed liquid weight ratio is 1:100, by extract solution in 65-70 DEG C of ultrafiltration membrance filter, residue is removed, biology is obtained Extracting flavonoids stoste, extracts stoste cryogenic vacuum by biological flavone and concentrates, and reclaims ethanol, and in 65-70 DEG C of spray drying, obtain Herba Saussureae Involueratae extract original powder.Wherein, Herba Saussureae Involueratae culture is aseptically, by vitro plant organ, tissue, thin Born of the same parents, embryo or Protoplast cuhnre are cultivated under conditions of artificial control on the culture medium manually prepared, organize it Or cell is bred and then obtained product is cultivated in progress as required, is ripe prior art.
In the present invention, as a kind of perferred technical scheme, low temperature, which is extracted, to be referred to extract at 25-35 DEG C.
In the present invention, as a kind of perferred technical scheme, the preparation method of the Ganoderma rich in chromium mycelium powder is as follows:
First stage zymotechnique flow:Fluid nutrient medium high-temperature sterilization, accesses liquid lucidum strain after cooling, import 100L first class seed pots, 25 DEG C of ferment at constant temperature under aerobic aseptic condition are cultivated 5 days, secondary seed tank are imported, aerobic sterile Under the conditions of 25 DEG C of constant temperature continue to cultivate 4 days, productivity fermentation tank is then introduced into, equally in 25 DEG C of constant temperature under aerobic aseptic condition Culture 4 days, fermentation ends obtain fermenation raw liquid;
Second stage extraction process flow:Fermenation raw liquid is boiled 2 hours using distilled water at 90-100 DEG C, is then introduced into Disk centrifugal pump, retains supernatant, removes residue, and cryogenic vacuum is concentrated 2-4 hours, obtains 15wt% polysaccharide concentrates, then Using 95wt% ethanol solution alcohol precipitations 6-8 hours, small-molecule substance is removed, is retained based on fungi polysaccharide, is reclaimed ethanol, utilize spray Mist seasoning obtains Ganoderma rich in chromium mycelium powder.
In the present invention, as a kind of perferred technical scheme, the fluid nutrient medium includes purified water, glucose, albumen Peptone, dusty yeast, micro- trivalent chromium and growth factor.
In the present invention, as a kind of perferred technical scheme, high-temperature sterilization is temperature to be maintained at 125 DEG C 1-2 hours.
In the present invention, as a kind of perferred technical scheme, cryogenic vacuum concentration is concentrated in vacuo at 20-25 DEG C.
In the present invention, black fruit Sorbus alnifloria (i.e. Black Box Tracing) belongs to perennial machaka, is a kind of emerging small berries.Really It is real to be rich in flavonoids and liposoluble ingredient, the ability with stronger antioxidation activity and removing free radical.It is big according to Chinese agriculture Learn Food Science and tested with key lab of functional food Beijing of nutrition engineering college and shown:Content of mineral substances in fresh fruit reaches 580mg/100g, wherein potassium content are particularly pertinent, and sodium is micro.B group micro-elements are complete abundant, carotenoid (beta carotene, jade Cream-coloured matter etc.) content is higher.Polyphenols is main functional activity material in black fruit Sorbus alnifloria, and Determination of Polyphenols is 3440- 7849mg/100g (dry weight).In the fruit of black fruit Sorbus alnifloria, containing abundant anthocyanidin, flavones, (fresh fruit content is up to 0.25%- 0.35%), polyphenol, also containing the material such as multivitamin and mineral element.Polyphenol is to improve the structure of capillary and blood vessel With the very important material of function, additionally aid stimulation and improve the circulatory system.Anthocyanidin and flavones are able to maintain that the heart of people The health of dirty and body.Research shows that black fruit Sorbus alnifloria leaf extract can stimulate PC12 cells and L929 cells to glucose Intake, significantly reduces the diabetes rat of streptozotocin (STZ) induction and the blood sugar level of normal rat.In clinical application research It is middle to find, black fruit Sorbus alnifloria fruit juice is taken after 3 months, and the fasting blood-glucose of type 2 diabetes patient is substantially reduced, it is sugared in patients blood plasma Change hemoglobin, lipid and total cholesterol level also to decrease, this shows that black fruit Sorbus alnifloria can be used for the auxiliary of diabetic Therapeutic diet.
Containing abundant biological flavone in Herba Saussureae Involueratae extract original powder, biological flavone main function is exactly to dissolve blood cake blood Bolt, quick effectively cracking separation patch, quickly removes thrombus, patch, cleans the toxin and lipid rubbish of blood kind, by recovering The pliability of capillary, makes the capillary of hardening recover elasticity.Saussurea involucrata culture is not only containing close with natural snow lotus Active component, and also there is many pharmacological activity suitable therewith.Its main pharmacological analgesia, anti-inflammatory, antirheumatic;Suppression Platelet aggregation processed, hypoglycemic, reducing blood lipid, improvement blood circulation;There is adjustment effect to immune system.
The pharmaceutical component of ganoderma lucidum mycelium powder enriches very much, and wherein active ingredient includes GL-B, ganoderma lucidum polypeptide, triterpene Class, 16 kinds of amino acid (wherein containing seven kinds of essential amino acids), protein, steroid, mannitol, coumarin glycosides, alkaloid, Organic acid (main contain fumaric acid), and trace element Ge, Fe, Ca, Mn, Zn, etc..Ganoderma lucidum has bidirectional modulation work to human body With controlling disease, be related to each system such as cardiovascular and cerebrovascular, digestion, nerve, endocrine, breathing, motion, especially to tumour, liver Lesion, insomnia and the preventive and therapeutic effect of aging are very notable.Micro- Organic Chromium is one of the essential trace elements of the human body, people Internal chromium is nearly all trivalent, and it is very stable, and containing about chromium 6mg (milligram) in human body, it is universally present in skeleton, flesh Among meat, hair, skin, hypodermis, major organs (except lung) and body fluid.The blood sugar reducing function mechanism of chromium is can to increase pancreas islet Disorderly adhesion, increase insulin receptor quantity, the phosphorylation of increase insulin receptor with acceptor etc..Suction of the human body to inorganic chromium Utilization rate is received extremely low, less than 1%;Human body is to the utilization rate of Organic Chromium up to 10~25%.The μ g of normal adult's demand 20~50 (microgram)/day, children, pregnant woman and old man are 50~110 μ g/ days, and diabetic and obese people are 50~200 μ g/ days.
The biological nutrition compound composition that the present invention is provided is the basic principle composition constituted according to traditional Chinese medical prescription compatibility 's.The medicine of composition prescription can be divided into monarch drug in a prescription, ministerial drug by its effect played in prescription, adjutant, make medicine, referred to as monarch, minister, Help, make.Wherein, monarch drug in a prescription is black fruit Sorbus alnifloria extract, and primary treatment is played to diabetes, can remove free radical, Tianshan Mountains snow Lotus extract original powder can play dissolving blood cake thrombus, reduce the effect of blood viscosity as ministerial drug, auxiliary monarch drug in a prescription treatment; Ganoderma lucidum mycelium powder coordinates monarch-minister drug treatment accompanying symptoms, improves hematoglobin protein synthesis capability as adjutant;And ganoderma lucidum mycelium powder In the trivalent chromium that is rich in can then play effect that collaboration insulin participates in human body sugar and lipid-metabolism together.It is several above Raw material is mutually cooperateed with and played a role jointly, plays the auxiliary therapeutic action to diabetes.And the whole preferably natural plants of formula, Ecological safety, rich in nutritional ingredient, without any potential health hazard.By rational dialectical compatibility, gradually reduce or replace In generation, takes the use of depressor and insulin injection, and eliminates or reduce the toxic side effect that medicine is brought, and reaches what slow disease was controlled soon Effect.
Inventor thinks:Optimization nutrition treatment is the primary measure of all kinds Diabetes Foundation treatment.By controlling meals Eat the intake of gross energy, the reasonable various nutriments of equilibrium assignment, to meet the physiological requirement of glycometabolism.If everyone passes through Health diet and increase amount of exercise, can reduce risk.In the case of no diabetic complication, by six months To the adjustment of 1 year, blood glucose can recover normal.
Special diet demand of the invention aiming at diabetes, the Polyphenols extracted using modern biotechnology science and technology fermentation, Flavonoids, fungi polysaccharide class and formula that is related micro- and preparing.The formula of these active ingredients, realizes following effect: Improve insulin sensitivity, softening and dredging blood vessel improves liver and renal glucose sugar generation and storage capacity, maintains carbohydate metabolism Balance.Reduce or offset simultaneously the side effect of traditional treatment diabetes.Moreover, inventor draws by a large amount of experiments repeatedly Specific extraction, fermentation process, the active ingredient of each component fully can be extracted and played a role, it is ensured that this several component The effect of synergy.
Due to using above technical scheme, the invention has the advantages that:
1st, ecological safety, is formulated the raw materials used new resource food for ratifying production all from health ministry, does not have Any potential health hazard;
2nd, formulation product sense organ and mouthfeel come from the true qualities of plant, and no chemistry addition allows people to be willing to accept.
3rd, the active constituent content of product is high, and effect is strong, and the slow disease that can reach auxiliary treatment controls effect soon;
4th, raw material needed for product is secure, there is production base, there is bioengineering production line, there is new-product development laboratory, The market demand can be ensured;
5th, the present invention is both adapted to diabetes mellitus prevention, and the disease that all kinds glycosuria is adapted to again is repaired and auxiliary treatment;
6th, during the formulation product is eaten, without any side effect.
Embodiment
Technical solution of the present invention is described in detail below in conjunction with embodiment.Following examples are only used for more clear Illustrate to Chu technical scheme, therefore be only used as example, and can not be limited the scope of the invention with this.
Embodiment 1
A kind of biological nutrition compound composition of auxiliary treatment diabetes, is prepared from by the raw material of following parts by weight:It is black 70 parts of fruit Sorbus alnifloria extract;Herba Saussureae Involueratae extract 15 parts of powder of original;15 parts of Ganoderma rich in chromium mycelium powder;Above raw material amounts to 100 Part.The content of chromium is 0.1wt% in the composition.Chromium is trivalent chromium, the edible Organic Chromium of state approval.
Wherein, black fruit Sorbus alnifloria extract is adopted prepares with the following method:
Take the fruit of black fruit Sorbus alnifloria to crush, be 1 according to solid-liquid ratio:5 add distilled water, are warming up to 50 DEG C, are kept for 3 minutes, Then it is 1 to add solid-liquid ratio:The volume ratio of ethanol and water is 1 in 3 ethanol solution, ethanol solution:1, adjustment pH is 3.5, Temperature is extracted 2 hours for heating water bath at 32 DEG C, extract solution is then centrifuged into 30min under 2000 revs/min of speed, and rise Warm to 45 DEG C are concentrated under reduced pressure, and are then spray-dried, obtain black fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract original powder is adopted to be prepared with the following method:
By Herba Saussureae Involueratae tissue culture using 70 DEG C of dehydrations of oven, low temperature drying 8 hours, are obtained at 35 DEG C Below water content 8wt% Herba Saussureae Involueratae tissue culture dry powder, 80 mesh are crushed to by Herba Saussureae Involueratae tissue culture dry powder, profit With the solution of 80wt% ethanol, low temperature is extracted at 25 DEG C, and feed liquid weight ratio is 1:100, by extract solution in 65 DEG C of milipore filter mistakes Filter, remove residue, obtain biological flavone and extract stoste, biological flavone is extracted into stoste cryogenic vacuum concentrates, recovery ethanol, and 65 DEG C of spray drying, obtain the former powder of Herba Saussureae Involueratae extract;
The preparation method of Ganoderma rich in chromium mycelium powder is as follows:
First stage zymotechnique flow:Fluid nutrient medium includes purified water, glucose, peptone, dusty yeast, micro member Plain trivalent chromium and growth factor, maintain temperature to carry out high-temperature sterilization in 1 hour fluid nutrient medium, are accessed after cooling at 125 DEG C Liquid lucidum strain, imports 100L first class seed pots, and 25 DEG C of ferment at constant temperature under aerobic aseptic condition are cultivated 5 days, import two grades Seeding tank, 25 DEG C of constant temperature continue to cultivate 4 days under aerobic aseptic condition, productivity fermentation tank are then introduced into, equally aerobic Lower 25 DEG C of aseptic condition is incubated 4 days, and fermentation ends obtain fermenation raw liquid;
Second stage extraction process flow:Fermenation raw liquid is boiled 2 hours using distilled water at 90 DEG C, dish-style is then introduced into Centrifugal pump, retains supernatant, removes residue, and cryogenic vacuum is concentrated 2 hours at 20 DEG C, obtains 15wt% polysaccharide concentrates, so 95wt% ethanol solutions alcohol precipitation is utilized afterwards 6 hours, remove small-molecule substance, retain based on fungi polysaccharide, reclaim ethanol, utilize spray Mist seasoning obtains Ganoderma rich in chromium mycelium powder.
The composition is prepared using following methods:
Weigh the former powder of black fruit Sorbus alnifloria extract, Herba Saussureae Involueratae extract, Ganoderma rich in chromium mycelium powder, tabletting, packaging.
Embodiment 2
A kind of biological nutrition compound composition of auxiliary treatment diabetes, is prepared from by the raw material of following parts by weight:It is black 50 parts of fruit Sorbus alnifloria extract;Herba Saussureae Involueratae extract 25 parts of powder of original;25 parts of Ganoderma rich in chromium mycelium powder;Above raw material amounts to 100 Part.The content of chromium is 0.05wt% in the composition.Chromium is trivalent chromium, edible organic of state approval Chromium.
Wherein, black fruit Sorbus alnifloria extract is adopted prepares with the following method:
Take the fruit of black fruit Sorbus alnifloria to crush, be 1 according to solid-liquid ratio:10 add distilled water, are warming up to 65 DEG C, are kept for 5 minutes, Then it is 1 to add solid-liquid ratio:The volume ratio of ethanol and water is 1 in 5 ethanol solution, ethanol solution:1, adjustment pH is 4.5, Temperature is extracted 3 hours for heating water bath at 35 DEG C, extract solution is then centrifuged into 45min under 2800 revs/min of speed, and rise Warm to 48 DEG C are concentrated under reduced pressure, and are then spray-dried, obtain black fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract original powder is adopted to be prepared with the following method:
By Herba Saussureae Involueratae tissue culture using 80 DEG C of dehydrations of oven, low temperature drying 10 hours, are obtained at 45 DEG C To below water content 8wt% Herba Saussureae Involueratae tissue culture dry powder, Herba Saussureae Involueratae tissue culture dry powder is crushed to 80 mesh, Using the solution of 80wt% ethanol, low temperature is extracted at 35 DEG C, and feed liquid weight ratio is 1:100, by extract solution in 70 DEG C of milipore filter mistakes Filter, remove residue, obtain biological flavone and extract stoste, biological flavone is extracted into stoste cryogenic vacuum concentrates, recovery ethanol, and 70 DEG C of spray drying, obtain the former powder of Herba Saussureae Involueratae extract;
The preparation method of Ganoderma rich in chromium mycelium powder is as follows:
First stage zymotechnique flow:Fluid nutrient medium includes purified water, glucose, peptone, dusty yeast, micro member Plain trivalent chromium and growth factor, maintain temperature to carry out high-temperature sterilization in 2 hours fluid nutrient medium, are accessed after cooling at 125 DEG C Liquid lucidum strain, imports 100L first class seed pots, and 25 DEG C of ferment at constant temperature under aerobic aseptic condition are cultivated 5 days, import two grades Seeding tank, 25 DEG C of constant temperature continue to cultivate 4 days under aerobic aseptic condition, productivity fermentation tank are then introduced into, equally aerobic Lower 25 DEG C of aseptic condition is incubated 4 days, and fermentation ends obtain fermenation raw liquid;
Second stage extraction process flow:Fermenation raw liquid is boiled 2 hours using distilled water at 100 DEG C, dish is then introduced into Formula centrifugal pump, retains supernatant, removes residue, and cryogenic vacuum is concentrated 4 hours at 25 DEG C, obtains 15wt% polysaccharide concentrates, Then utilize 95wt% ethanol solutions alcohol precipitation 8 hours, remove small-molecule substance, retain based on fungi polysaccharide, reclaim ethanol, utilize Spray drying process obtains Ganoderma rich in chromium mycelium powder.
The composition is prepared using following methods:
Weigh the former powder of black fruit Sorbus alnifloria extract, Herba Saussureae Involueratae extract, Ganoderma rich in chromium mycelium powder, tabletting, packaging.
Embodiment 3
A kind of biological nutrition compound composition of auxiliary treatment diabetes, is prepared from by the raw material of following parts by weight:By The raw material of following parts by weight is prepared from:Black 60 parts of fruit Sorbus alnifloria extract;Herba Saussureae Involueratae extract 20 parts of powder of original;Ganoderma rich in chromium bacterium 20 parts of filament powder.The content of chromium is 0.1-0.05wt% in the composition.Chromium is trivalent chromium, and state approval can be with Edible Organic Chromium.
Wherein, black fruit Sorbus alnifloria extract is adopted prepares with the following method:
Take the fruit of black fruit Sorbus alnifloria to crush, be 1 according to solid-liquid ratio:8 add distilled water, are warming up to 55 DEG C, are kept for 4 minutes, Then it is 1 to add solid-liquid ratio:The volume ratio of ethanol and water is 1 in 4 ethanol solution, ethanol solution:1, adjustment pH is 4, in temperature Spend and extracted 2.5 hours for heating water bath at 33 DEG C, extract solution is then centrifuged into 40min under 2500 revs/min of speed, and rise Warm to 46 DEG C are concentrated under reduced pressure, and are then spray-dried, obtain black fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract original powder is adopted to be prepared with the following method:
By Herba Saussureae Involueratae tissue culture using 75 DEG C of dehydrations of oven, low temperature drying 9 hours, are obtained at 40 DEG C Below water content 8wt% Herba Saussureae Involueratae tissue culture dry powder, 80 mesh are crushed to by Herba Saussureae Involueratae tissue culture dry powder, profit With the solution of 80wt% ethanol, low temperature is extracted at 30 DEG C, and feed liquid weight ratio is 1:100, by extract solution in 68 DEG C of milipore filter mistakes Filter, remove residue, obtain biological flavone and extract stoste, biological flavone is extracted into stoste cryogenic vacuum concentrates, recovery ethanol, and 68 DEG C of spray drying, obtain the former powder of Herba Saussureae Involueratae extract;
The preparation method of Ganoderma rich in chromium mycelium powder is as follows:
First stage zymotechnique flow:Fluid nutrient medium includes purified water, glucose, peptone, dusty yeast, micro member Plain trivalent chromium and growth factor, maintain temperature to carry out high-temperature sterilization in 1.5 hours fluid nutrient medium, cooling is followed by 125 DEG C Enter liquid lucidum strain, import 100L first class seed pots, 25 DEG C of ferment at constant temperature under aerobic aseptic condition are cultivated 5 days, import two Level seeding tank, 25 DEG C of constant temperature continue to cultivate 4 days under aerobic aseptic condition, are then introduced into productivity fermentation tank, are equally having Lower 25 DEG C of oxygen aseptic condition is incubated 4 days, and fermentation ends obtain fermenation raw liquid;
Second stage extraction process flow:Fermenation raw liquid is boiled 2 hours using distilled water at 95 DEG C, dish-style is then introduced into Centrifugal pump, retains supernatant, removes residue, and cryogenic vacuum is concentrated 3 hours at 23 DEG C, obtains 15wt% polysaccharide concentrates, so 95wt% ethanol solutions alcohol precipitation is utilized afterwards 7 hours, remove small-molecule substance, retain based on fungi polysaccharide, reclaim ethanol, utilize spray Mist seasoning obtains Ganoderma rich in chromium mycelium powder.
The composition is prepared using following methods:
Weigh the former powder of black fruit Sorbus alnifloria extract, Herba Saussureae Involueratae extract, Ganoderma rich in chromium mycelium powder, tabletting, packaging.
Embodiment 4
A kind of biological nutrition compound composition of auxiliary treatment diabetes, is prepared from by the raw material of following parts by weight:It is black 60 parts of fruit Sorbus alnifloria extract;Herba Saussureae Involueratae extract 15 parts of powder of original;25 parts of Ganoderma rich in chromium mycelium powder.Chromium member in the composition The content of element is 0.1-0.05wt%.Chromium is trivalent chromium, the edible Organic Chromium of state approval.
Wherein, black fruit Sorbus alnifloria extract is adopted prepares with the following method:
Take the fruit of black fruit Sorbus alnifloria to crush, be 1 according to solid-liquid ratio:5 add distilled water, are warming up to 65 DEG C, are kept for 3 minutes, Then it is 1 to add solid-liquid ratio:The volume ratio of ethanol and water is 1 in 5 ethanol solution, ethanol solution:1, adjustment pH is 4, in temperature Spend and extracted 2 hours for heating water bath at 35 DEG C, extract solution is then centrifuged into 30min under 2100 revs/min of speed, and heat up It is concentrated under reduced pressure, is then spray-dried to 48 DEG C, obtains black fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract original powder is adopted to be prepared with the following method:
By Herba Saussureae Involueratae tissue culture using 72 DEG C of dehydrations of oven, low temperature drying 8 hours, are obtained at 45 DEG C Below water content 8wt% Herba Saussureae Involueratae tissue culture dry powder, 80 mesh are crushed to by Herba Saussureae Involueratae tissue culture dry powder, profit With the solution of 80wt% ethanol, low temperature is extracted at 35 DEG C, and feed liquid weight ratio is 1:100, by extract solution in 65 DEG C of milipore filter mistakes Filter, remove residue, obtain biological flavone and extract stoste, biological flavone is extracted into stoste cryogenic vacuum concentrates, recovery ethanol, and 67 DEG C of spray drying, obtain the former powder of Herba Saussureae Involueratae extract;
The preparation method of Ganoderma rich in chromium mycelium powder is as follows:
First stage zymotechnique flow:Fluid nutrient medium includes purified water, glucose, peptone, dusty yeast, micro member Plain trivalent chromium and growth factor, maintain temperature to carry out high-temperature sterilization in 1.3 hours fluid nutrient medium, cooling is followed by 125 DEG C Enter liquid lucidum strain, import 100L first class seed pots, 25 DEG C of ferment at constant temperature under aerobic aseptic condition are cultivated 5 days, import two Level seeding tank, 25 DEG C of constant temperature continue to cultivate 4 days under aerobic aseptic condition, are then introduced into productivity fermentation tank, are equally having Lower 25 DEG C of oxygen aseptic condition is incubated 4 days, and fermentation ends obtain fermenation raw liquid;
Second stage extraction process flow:Fermenation raw liquid is boiled 2 hours using distilled water at 92 DEG C, dish-style is then introduced into Centrifugal pump, retains supernatant, removes residue, and cryogenic vacuum is concentrated 3.5 hours at 25 DEG C, obtains 15wt% polysaccharide concentrates, Then utilize 95wt% ethanol solutions alcohol precipitation 7 hours, remove small-molecule substance, retain based on fungi polysaccharide, reclaim ethanol, utilize Spray drying process obtains Ganoderma rich in chromium mycelium powder.
The composition is prepared using following methods:
Weigh the former powder of black fruit Sorbus alnifloria extract, Herba Saussureae Involueratae extract, Ganoderma rich in chromium mycelium powder, tabletting, during tabletting, plus Enter microcrystalline cellulose as auxiliary material, packaging.
Measure of merit embodiment
30 mild diabetes patients (blood glucose is 7.9 or so on an empty stomach) are randomly divided into tri- groups of A, B, C, wherein, A groups clothes The biological nutrition compound composition provided with the present invention, each three of morning and afternoon, chews or swallows by daily 6;It is most that B groups, which are taken, Conventional gram sugar profit;C groups do not take any medicament.
As a result it is as follows:
Project Persistently take average blood sugar after 5 weeks Disable average blood sugar after 3 weeks
A groups 5.7 6.2
B groups 4.5 6.7
C groups 7.9 8.1
As can be seen from the above results:
The biological nutrition compound composition that the present invention is provided has relatively more significant effect for regulation hyperglycaemia, although clothes With the blood sugar decreasing effect of period not as a gram sugar profit, but after being discontinued, blood glucose rise is slower, also illustrates have more for blood glucose Long-acting adjustment effect.
Use embodiment
Certain is shut out, man, 50 years old, people from Haikou, physical examination blood glucose on July 8 (empty stomach) 7.48 (normal 3.9-6) in 2016;Paddy Aminoacyl transpeptidase 188 (normal 10-60), belongs to diabetic, takes the composition of the present invention, daily 6, July 9 in 2016 Day starts to eat, and 8.1 is down to 6.3 on an empty stomach by original after first quarter moon, effect is obvious.
Finally it should be noted that:Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations;To the greatest extent The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that:Its according to The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered Row equivalent substitution;And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology The scope of scheme, it all should cover among the claim of the present invention and the scope of specification.

Claims (10)

1. a kind of biological nutrition compound composition of auxiliary treatment diabetes, it is characterised in that by the raw material system of following parts by weight It is standby to form:
Black 50-70 parts of fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract 15-25 parts of powder of original;
15-25 parts of Ganoderma rich in chromium mycelium powder;
Above raw material amounts to 100 parts.
2. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 1, it is characterised in that by The raw material of following parts by weight is prepared from:
Black 60 parts of fruit Sorbus alnifloria extract;
Herba Saussureae Involueratae extract 20 parts of powder of original;
20 parts of Ganoderma rich in chromium mycelium powder.
3. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 1, it is characterised in that institute The content for stating chromium in composition is 0.1-0.05wt%.
4. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 1, it is characterised in that institute Composition is stated to prepare using following methods:
Weigh the former powder of black fruit Sorbus alnifloria extract, Herba Saussureae Involueratae extract, Ganoderma rich in chromium mycelium powder, tabletting, packaging.
5. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 1, it is characterised in that institute Black fruit Sorbus alnifloria extract is stated to prepare using following methods:Take the fruit of black fruit Sorbus alnifloria to crush, be 1 according to solid-liquid ratio:5-10 is added Distilled water, is warming up to 50-65 DEG C, is kept for 3-5 minutes, it is 1 then to add solid-liquid ratio:3-5 ethanol solution, adjustment pH is 3.5- 4.5, it is that heating water bath is extracted 2-3 hours at 32-35 DEG C in temperature, the then speed by extract solution at 2000-2800 revs/min Lower centrifugation 30-45min, and be warming up to 45-48 DEG C and be concentrated under reduced pressure, then it is spray-dried, obtains black fruit Sorbus alnifloria extract.
6. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 5, it is characterised in that second The volume ratio of ethanol and water is 1 in alcoholic solution:1.
7. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 1, it is characterised in that day The preparation method of the former powder of mountain Saussurea involucrata extract is as follows:Herba Saussureae Involueratae tissue culture is utilized into 70-80 DEG C of dehydration of oven Low temperature drying 8-10 hours, obtains below water content 8wt% Herba Saussureae Involueratae tissue culture dry powder, Herba Saussureae Involueratae tissue is trained Support thing dry powder and be crushed to 80 mesh, extracted using the solution low temperature of 80wt% ethanol, feed liquid weight ratio is 1:100, extract solution is existed 65-70 DEG C of ultrafiltration membrance filter, removes residue, obtains biological flavone and extracts stoste, and it is dense that biological flavone is extracted into stoste cryogenic vacuum Contracting, reclaims ethanol, and in 65-70 DEG C of spray drying, obtains the former powder of Herba Saussureae Involueratae extract.
8. the biological nutrition compound composition of a kind of auxiliary treatment diabetes according to claim 7, it is characterised in that low Temperature, which is extracted, to be referred to extract at 25-35 DEG C.
9. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 1, it is characterised in that institute The preparation method for stating Ganoderma rich in chromium mycelium powder is as follows:
First stage zymotechnique flow:Fluid nutrient medium high-temperature sterilization, accesses liquid lucidum strain after cooling, import 100L mono- Level seeding tank, 25 DEG C of ferment at constant temperature under aerobic aseptic condition are cultivated 5 days, secondary seed tank are imported, under aerobic aseptic condition 25 DEG C of constant temperature continue to cultivate 4 days, productivity fermentation tank are then introduced into, equally in 25 DEG C incubated 4 under aerobic aseptic condition My god, fermentation ends obtain fermenation raw liquid;
Second stage extraction process flow:Fermenation raw liquid is boiled 2 hours using distilled water at 90-100 DEG C, dish-style is then introduced into Centrifugal pump, retains supernatant, removes residue, and cryogenic vacuum is concentrated 2-4 hours, is obtained 15wt% polysaccharide concentrates, is then utilized 95wt% ethanol solution alcohol precipitations 6-8 hours, remove small-molecule substance, retain based on fungi polysaccharide, reclaim ethanol, dry using spraying Dry method obtains Ganoderma rich in chromium mycelium powder.
10. a kind of biological nutrition compound composition of auxiliary treatment diabetes according to claim 9, it is characterised in that The fluid nutrient medium includes purified water, glucose, peptone, dusty yeast, micro- trivalent chromium and growth factor.
CN201710134651.3A 2017-03-08 2017-03-08 A kind of biological nutrition compound composition of auxiliary treatment diabetes Pending CN107095300A (en)

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