CN106967619A - A kind of preparation method of Medium for Ganoderma lucidum and ganoderma lucidum mycelium - Google Patents
A kind of preparation method of Medium for Ganoderma lucidum and ganoderma lucidum mycelium Download PDFInfo
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- CN106967619A CN106967619A CN201710278115.0A CN201710278115A CN106967619A CN 106967619 A CN106967619 A CN 106967619A CN 201710278115 A CN201710278115 A CN 201710278115A CN 106967619 A CN106967619 A CN 106967619A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention relates to fungus growing technique field, and in particular to a kind of preparation method of Medium for Ganoderma lucidum and ganoderma lucidum mycelium, the Medium for Ganoderma lucidum includes the raw material components of following parts by weight：50~150 parts of wood chip, 10~40 parts of wheat bran, 0.1~10 part of land plaster, 0.1~10 part of sugar and 0.0001~0.001 part of vitamin B2.A kind of Medium for Ganoderma lucidum and the preparation method of ganoderma lucidum mycelium that the present invention is provided, composition of raw materials compatibility are scientific and reasonable, and the ganoderma lucidum mycelium activity obtained based on the culture medium inoculated is stronger, and rate of vaccination is higher, and then improve ganoderma lucidum plantation yield.The Medium for Ganoderma lucidum and the preparation method of ganoderma lucidum mycelium that the present invention is provided simultaneously have the advantages that efficient, cheap, simple to operate, practical.
Description
Technical field
The present invention relates to fungus growing technique field, and in particular to a kind of preparation of Medium for Ganoderma lucidum and ganoderma lucidum mycelium
Method.
Background technology
Ganoderma lucidum belongs to the fructification of On Polyporaceae ganoderma lucidum.With invigorating qi for tranquilization, the relieving cough and asthma, work(promoted longevity
Effect.The medicinal history existing more than 2000 years in China of ganoderma lucidum, the mystery for be considered as strengthening by means of tonics by successive dynasties medical family, strengthening the body resistance to consolidate the constitution is precious
Product.Modern medicine study finds that the nutritional ingredient of ganoderma lucidum is very enriched, active ingredient may include ganodenic acid, GL-B,
Ganoderma lucidum polypeptide, 16 kinds of amino acid (wherein containing seven kinds of amino acid needed by human), steroid, mannitol, coumarin glycosides, biologies
Alkali, organic acid (main to contain fumaric acid), and trace element Ge, P, Fe, Ca, Mn, Zn etc..It has bidirectional modulation to human body
Effect, conditioning engine body internal balance, adjusts the metabolic function of human body, improves the immunocompetence of itself, especially to swollen
Knurl, hepar damnification, have a sleepless night aging preventive and therapeutic effect it is more notable.
In ganoderma lucidum product increasing today, the making and research and development of lucidum strain are particularly important, its yield, matter
None management tactics for not affecting the producer such as amount, input, output and the purchase intention of consumer.At present, lucidum strain is trained
Material is supported, is mainly made up of wood chip, wood chip, land plaster, sugar, water, the culture medium of the formula is easily moved back in the course of cultivation
Change, show as that mycelial growth is weak, vitality is not strong, and resistance is poor, and pest and disease damage is serious.
As can be seen here, deficiency of the prior art can be based on there is provided a kind of improved Medium for Ganoderma lucidum of composition of raw materials, and
The preparation method of ganoderma lucidum mycelium based on the culture medium, effectively solves to occur in ganoderma lucidum large-scale production even factory culture
The reduction of strain resistance, activity is not enough, vitality is poor, the problems such as yield poorly, urgently to be resolved hurrily as those skilled in the art
Technical barrier.
The content of the invention
The present invention in order to solve the above-mentioned technical problem there is provided the preparation method of a kind of Medium for Ganoderma lucidum and ganoderma lucidum mycelium,
The present invention is by adding appropriate vitamin B2 and scientific and reasonable compatibility other raw material.To significantly improve lucidum strain activity and connect
Success rate is planted, the Ganoderma Lucidum linden survival rate and yield being inoculated with out are higher.
In order to reach above-mentioned technique effect, the present invention includes following technical scheme：
A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：50~150 parts of wood chip, 10~40 parts
Wheat bran, 0.1~10 part of land plaster, 0.1~10 part of sugar and 0.0001~0.001 part of vitamin B2.
The above-mentioned each composition of raw materials of reasonable compatibility of the present invention, as the raw material components of Medium for Ganoderma lucidum, passes through the appropriate dimension of addition
Raw element B2, enhancing bacterial activity and rate of vaccination, and then improve ganoderma lucidum plantation yield.It has efficient, cheap, simple to operate, real
With property it is strong the features such as.
Further, the raw material components of following parts by weight are included：60~100 parts of wood chip, 15~35 parts of wheat bran,
0.5~5 part of land plaster, 0.5~5 part of sugar and 0.0002~0.0005 part of vitamin B2.
The content value of further preferably above-mentioned raw materials formula of the invention, so as to get Medium for Ganoderma lucidum it is more excellent.
Further, the raw material components of following parts by weight are included：0.01~0.5 part of magnesium sulfate, 0.01~0.5 part
Calcium superphosphate and 0.01~0.5 part of KI.
The present invention further has preferred above-mentioned raw materials component to carry out compatibility, so as to get medium nutrient content enrich, fill
Divide and meet ganoderma lucidum growth needs, greatly enhance bacterial activity and rate of vaccination.
Further, the raw material components of following parts by weight are included：5~20 parts of wheat embryo and 10~30 parts of fruit
Vegetable skin.
The present invention further has preferred above-mentioned raw materials component to carry out compatibility, combines the nutrient of above-mentioned raw materials component, makes it
Nutrition, function and effect can be protruded more, and its health care and diseases prevention, cure the disease and other effects can be more notable.
Further, the wood chip is broad-leaved class hardwood wood chip, and the fruits and vegetables skin includes apple skin, banana skin and flue
One or more in pericarp.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, 25 DEG C~30 DEG C of temperature is placed in, humidity 60~65%
Under the conditions of culture obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Further, the preparation method of the Medium for Ganoderma lucidum comprises the following steps：Wheat is uniformly sprinkled after wood chip is shakeout
Bran and land plaster obtain wood chip mixture, and sugar is configured into syrup, and the vitamin B2 of crushing is dissolved in syrup, will be dissolved with
The syrup of vitamin B2 is uniformly sprinkling upon the surface of wood chip mixture, and wood chip mixture is rolled while spreading, and makes each original
Material component is sufficiently mixed, and it is 50~60% to make the moisture content in medium finally given.
To hold wood chip, just not water outlet is advisable the culture medium finally given.
Further, magnesium sulfate, calcium superphosphate and KI are also dissolved with the syrup.
Further, wheat embryo and fruits and vegetables skin are also uniformly sprinkled with after wood chip is shakeout.
Further, the fruits and vegetables skin need to be preprocessed, specifically includes following steps：Take fruits and vegetables skin is cleaned to dry laggard
Row pulverization process obtains pretreated fruits and vegetables skin, and pretreated fruits and vegetables skin is uniformly spread to wood chip.
Using above-mentioned technical proposal, including following beneficial effect：A kind of Medium for Ganoderma lucidum and Ganoderma Lucidum that the present invention is provided
The preparation method of filament, composition of raw materials compatibility is scientific and reasonable, and the ganoderma lucidum mycelium activity obtained based on the culture medium inoculated is stronger,
And rate of vaccination is higher, and then improve ganoderma lucidum plantation yield.The present invention is provided simultaneously Medium for Ganoderma lucidum and the system of ganoderma lucidum mycelium
Preparation Method has the advantages that efficient, cheap, simple to operate, practical.
Embodiment
The present invention is described in further detail below by specific embodiment.
Embodiment one：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：50 parts of wood chip, 40 parts
Wheat bran, 0.1 part of land plaster, 10 parts of sugar and 0.0001 part of vitamin B2.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, training under conditions of 25 DEG C DEG C of temperature, humidity 65% is placed in
Support and obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment two：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：60 parts of wood chip, 35 parts
Wheat bran, 0.5 part of land plaster, 5 parts of sugar and 0.0002 part of vitamin B2.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 28 DEG C of temperature, humidity 62% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment three：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：70 parts of wood chip, 30 parts
Wheat bran, 1 part of land plaster, 4 parts of sugar, 0.00022 part of vitamin B2,0.01 part of magnesium sulfate, 0.5 part of calcium superphosphate and
0.01 part of KI.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Wheat bran is uniformly sprinkled after wood chip is shakeout and land plaster obtains wood chip mixture,
Sugar is configured to syrup, the vitamin B2 of crushing, magnesium sulfate, calcium superphosphate and KI are dissolved in syrup, will be dissolved with
The syrup of vitamin B2 is uniformly sprinkling upon the surface of wood chip mixture, and wood chip mixture is rolled while spreading, and makes each original
Material component is sufficiently mixed, and it is 50% to make the moisture content in medium finally given；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 29 DEG C of temperature, humidity 61% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Example IV：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：80 parts of wood chip, 25 parts
Wheat bran, 2 parts of land plaster, 3 parts of sugar, 0.0001 part of vitamin B2,0.1 part of magnesium sulfate, 0.2 part of calcium superphosphate, 0.1
The KI, 5 parts of wheat embryo and 10 parts of fruits and vegetables skin of part.
The wood chip is broad-leaved class hardwood wood chip, and the fruits and vegetables skin is included in apple skin, banana skin and pitaya peel
It is one or more of
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Wheat bran, land plaster, wheat embryo and fruits and vegetables are uniformly sprinkled after wood chip is shakeout
Skin obtains wood chip mixture, and sugar is configured into syrup, and the vitamin B2 of crushing, magnesium sulfate, calcium superphosphate and KI are dissolved
Into syrup, the syrup for being dissolved with vitamin B2 is uniformly sprinkling upon to the surface of wood chip mixture, wood chip mixed while spreading
Thing is rolled, and is sufficiently mixed each raw material components, and it is 60% to make the moisture content in medium finally given；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 30 DEG C of temperature, humidity 60% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment five：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：78 parts of wood chip, 20 parts
Wheat bran, 1 part of land plaster, 1 part of sugar, 0.00025 part of vitamin B2,0.25 part of magnesium sulfate, 0.25 part of calcium superphosphate,
0.25 part of KI, 10 parts of wheat embryo and 20 parts of fruits and vegetables skin.
The wood chip is broad-leaved class hardwood wood chip, and the fruits and vegetables skin is included in apple skin, banana skin and pitaya peel
It is one or more of.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Pulverization process, which is carried out, after taking that fruits and vegetables skin is cleaned and drying obtains pretreated
Fruits and vegetables skin, pretreated fruits and vegetables skin is uniformly spread to wood chip；Wheat bran, land plaster, wheat are uniformly sprinkled after wood chip is shakeout
Plumule and pretreated fruits and vegetables skin obtain wood chip mixture, and sugar is configured into syrup, by the vitamin B2 of crushing, magnesium sulfate,
Calcium superphosphate and KI are dissolved in syrup, and the syrup for being dissolved with vitamin B2 is uniformly sprinkling upon to the surface of wood chip mixture,
Wood chip mixture is rolled while spreading, each raw material components are sufficiently mixed, makes the moisture content in medium finally given
For 55%；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 28 DEG C of temperature, humidity 64% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment six：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：90 parts of wood chip, 30 parts
Wheat bran, 4 parts of land plaster, 1 part of sugar, 0.0003 part of vitamin B2,0.2 part of magnesium sulfate, 0.3 part of calcium superphosphate, 0.1
The KI, 20 parts of wheat embryo and 30 parts of fruits and vegetables skin of part.
The wood chip is broad-leaved class hardwood wood chip, and the fruits and vegetables skin is included in apple skin, banana skin and pitaya peel
It is one or more of.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Pulverization process, which is carried out, after taking that fruits and vegetables skin is cleaned and drying obtains pretreated
Fruits and vegetables skin, pretreated fruits and vegetables skin is uniformly spread to wood chip；Wheat bran, land plaster, wheat are uniformly sprinkled after wood chip is shakeout
Plumule and pretreated fruits and vegetables skin obtain wood chip mixture, and sugar is configured into syrup, by the vitamin B2 of crushing, magnesium sulfate,
Calcium superphosphate and KI are dissolved in syrup, and the syrup for being dissolved with vitamin B2 is uniformly sprinkling upon to the surface of wood chip mixture,
Wood chip mixture is rolled while spreading, each raw material components are sufficiently mixed, makes the moisture content in medium finally given
For 58%；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 25 DEG C of temperature, humidity 62% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment seven：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：90 parts of wood chip, 20 parts
Wheat bran, 4 parts of land plaster, 1 part of sugar, 0.0004 part of vitamin B2,0.5 part of magnesium sulfate, 0.01 part of calcium superphosphate and
0.5 part of KI.
The wood chip is broad-leaved class hardwood wood chip.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Wheat bran is uniformly sprinkled after wood chip is shakeout and land plaster obtains wood chip mixture,
Sugar is configured to syrup, the vitamin B2 of crushing, magnesium sulfate, calcium superphosphate and KI are dissolved in syrup, will be dissolved with
The syrup of vitamin B2 is uniformly sprinkling upon the surface of wood chip mixture, and wood chip mixture is rolled while spreading, and makes each original
Material component is sufficiently mixed, and it is 59% to make the moisture content in medium finally given；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 28 DEG C of temperature, humidity 62% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment eight：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：100 parts of wood chip, 15 parts
Wheat bran, 5 parts of land plaster, 0.5 part of sugar and 0.0005 part of vitamin B2.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Wheat bran is uniformly sprinkled after wood chip is shakeout and land plaster obtains wood chip mixture,
Sugar is configured to syrup, the vitamin B2 of crushing, magnesium sulfate, calcium superphosphate and KI are dissolved in syrup, will be dissolved with
The syrup of vitamin B2 is uniformly sprinkling upon the surface of wood chip mixture, and wood chip mixture is rolled while spreading, and makes each original
Material component is sufficiently mixed, and it is 60% to make the moisture content in medium finally given；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 30 DEG C of temperature, humidity 63% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment nine：A kind of Medium for Ganoderma lucidum, includes the raw material components of following parts by weight：150 parts of wood chip, 10 parts
Wheat bran, 10 parts of land plaster, 0.1 part of sugar and 0.001 part of vitamin B2.
A kind of preparation method of ganoderma lucidum mycelium, comprises the following steps：
Step one：Prepare Medium for Ganoderma lucidum：Wheat bran is uniformly sprinkled after wood chip is shakeout and land plaster obtains wood chip mixture,
Sugar is configured to syrup, the vitamin B2 of crushing, magnesium sulfate, calcium superphosphate and KI are dissolved in syrup, will be dissolved with
The syrup of vitamin B2 is uniformly sprinkling upon the surface of wood chip mixture, and wood chip mixture is rolled while spreading, and makes each original
Material component is sufficiently mixed, and it is 55% to make the moisture content in medium finally given；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, culture under conditions of 28 DEG C of temperature, humidity 65% is placed in
Obtain ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is above-mentioned Medium for Ganoderma lucidum.
Embodiment one is to the Mycelium Growth of Ganoderma lucidum situation test experience of embodiment nine：
Successively using the Medium for Ganoderma lucidum described in embodiment one to embodiment nine, using the ganoderma lucidum mycelium of the culture medium
Preparation method carry out ganoderma lucidum mycelium cultivation experimental group the most, while using conventional medium carry out ganoderma lucidum mycelium culture as
Control group, observes and records control group and the growing state of experimental group mycelia daily, growing way from mycelia, dense, neat and clean
Mycelial growth situation is investigated in terms of the appearance characters such as whiteness and mycelial growth rate.Experimental result is shown in Table 1.
The Mycelium Growth of Ganoderma lucidum situation testing result of table 1
As known from Table 1, the ganoderma lucidum mycelium prepared by above-described embodiment one to the Medium for Ganoderma lucidum of embodiment nine, from mycelia
The speed of growth, outward appearance and mycelium growth vigor will become apparent from its resistance, activity and be significantly improved compared to conventional medium.Together
When most preferred embodiment be embodiment five.
The comparison of ganoderma lucidum fruitbody character and yield：
Example one to embodiment nine cultivates obtained ganoderma lucidum mycelium, and further progress, which is cultivated, obtains ganoderma lucidum in fact
Body, records and sees successively
Examine the shape and output condition of corresponding ganoderma lucidum fruitbody.Testing result is shown in Table 2.
The ganoderma lucidum fruitbody character of table 2 and yield testing result
As known from Table 2, the spirit that the ganoderma lucidum mycelium that the embodiment of the present invention one is prepared to embodiment nine is further cultivated
Sesame fructification, bacteria cover diameter, cap thickness, monolithic fresh weight, yield, biological transformation ratio are obvious compared to what conventional medium was present
Advantage.Its bacteria cover diameter, thickness, yield and biological transformation ratio can be seen that by the present invention from the economical character of fructification
Each item data is significantly improved after method culture, and single bag produces intensive, and yield is significantly improved.It is wherein most preferably real
Apply example five.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (10)
1. a kind of Medium for Ganoderma lucidum, it is characterised in that include the raw material components of following parts by weight：50~150 parts of wood chip, 10
~40 parts of wheat bran, 0.1~10 part of land plaster, 0.1~10 part of sugar and 0.0001~0.001 part of vitamin B2.
2. Medium for Ganoderma lucidum according to claim 1, it is characterised in that include the raw material components of following parts by weight：60
~100 parts of wood chip, 15~35 parts of wheat bran, 0.5~5 part of land plaster, 0.5~5 part of sugar and 0.0002~0.0005 part
Vitamin B2.
3. Medium for Ganoderma lucidum according to claim 1, it is characterised in that also include the raw material components of following parts by weight：
0.01~0.5 part of magnesium sulfate, 0.01~0.5 part of calcium superphosphate and 0.01~0.5 part of KI.
4. the Medium for Ganoderma lucidum according to any one of claims 1 to 3, it is characterised in that also including following parts by weight
Raw material components：5~20 parts of wheat embryo and 10~30 parts of fruits and vegetables skin.
5. Medium for Ganoderma lucidum according to claim 4, it is characterised in that the wood chip is broad-leaved class hardwood wood chip, institute
Stating fruits and vegetables skin includes the one or more in apple skin, banana skin and pitaya peel.
6. a kind of preparation method of ganoderma lucidum mycelium, it is characterised in that comprise the following steps：
Step one：Prepare Medium for Ganoderma lucidum；
Step 2：Sterilizing：Take the culture medium of preparation to be placed in cultivation bag, carry out sterilization treatment；
Step 3：Inoculation：Aseptically, to inoculation ganoderma lucidum second class inoculum on culture medium；
Step 4：Culture：The ganoderma lucidum three-class strain after inoculation is taken, 25 DEG C~30 DEG C of temperature, the condition of humidity 60~65% is placed in
Lower culture obtains ganoderma lucidum mycelium.
The Medium for Ganoderma lucidum is the Medium for Ganoderma lucidum described in any one of Claims 1 to 5.
7. require the preparation method of the ganoderma lucidum mycelium described in 6 according to going there, it is characterised in that the system of the Medium for Ganoderma lucidum
Preparation Method comprises the following steps：Wheat bran is uniformly sprinkled after wood chip is shakeout and land plaster obtains wood chip mixture, sugar is configured to
Syrup, the vitamin B2 of crushing is dissolved in syrup, and the syrup for being dissolved with vitamin B2 is uniformly sprinkling upon into wood chip mixture
Surface, rolls while spreading to wood chip mixture, is sufficiently mixed each raw material components, contains the culture medium finally given
Water is 50~60%.
8. the preparation method of ganoderma lucidum mycelium according to claim 7, it is characterised in that be also dissolved with sulphur in the syrup
Sour magnesium, calcium superphosphate and KI.
9. the preparation method of ganoderma lucidum mycelium according to claim 7, it is characterised in that also uniformly spread after wood chip is shakeout
There are wheat embryo and fruits and vegetables skin.
10. the preparation method of ganoderma lucidum mycelium according to claim 9, it is characterised in that the fruits and vegetables skin need to be through pre- place
Reason, specifically includes following steps：Pulverization process, which is carried out, after taking that fruits and vegetables skin is cleaned and drying obtains pretreated fruits and vegetables skin, will be pre-
Fruits and vegetables skin after processing is uniformly spread to wood chip.
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| CN108029952A (en) * | 2017-12-04 | 2018-05-15 | 王广彦 | It is a kind of to be used to cultivate the culture medium of ganoderma lucidum mycelium and manufactured ganoderma lucidum mycelium |
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| CN108029952A (en) * | 2017-12-04 | 2018-05-15 | 王广彦 | It is a kind of to be used to cultivate the culture medium of ganoderma lucidum mycelium and manufactured ganoderma lucidum mycelium |
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