CN105123270A - Basswood ganoderma lucidum planting method - Google Patents
Basswood ganoderma lucidum planting method Download PDFInfo
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- CN105123270A CN105123270A CN201510553250.2A CN201510553250A CN105123270A CN 105123270 A CN105123270 A CN 105123270A CN 201510553250 A CN201510553250 A CN 201510553250A CN 105123270 A CN105123270 A CN 105123270A
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- linden
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- basswood
- bacterial classification
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Abstract
The invention discloses a basswood ganoderma lucidum planting method. The method comprises the steps of basswood processing, basswood block sterilization, wild ganoderma lucidum strain liquid preparation, inoculation, strain culture and follow-up management. During basswood block sterilization, basswood blocks are placed in a mixture to be heated and sterilized, wherein the mixture is prepared from 4% of radix sophorae flavescentis, 2% of neem, 0.7% of golden cypress, 0.23% of phoenix-tail fern, 1.5% of salt, 0.08% of betel nuts, 0.05% of annao pills, 0.028% of cortex lycii radicis, 0.015% of acne-fighting cream, 0.008% of patrinia heterophylla, 0.006% of cyrtomium fortune and the balance water. The method has the advantages that the influence of weather is quite small, operation is convenient, using is flexible, and cultivated ganoderma lucidum looks good and symmetrical and is rarely attacked by pests.
Description
Technical field
The present invention relates to agricultural food product production technical field, specifically, is the implantation methods of a kind of linden glossy ganoderma.
Background technology
Glossy ganoderma, profile is umbrella, cap kidney shape, semicircle or subcircular, is the fruit body of On Polyporaceae glossy ganoderma.There is invigorating qi for tranquilization, relieving cough and asthma effect, cough breathe heavily for dizzy egersis, palpitation, consumptive disease.Glossy ganoderma master is containing amino acid, polypeptide, protein, fungal lysozyme, and carbohydrate (reducing sugar and polysaccharide), ergosterol, triterpenes, coumarin glucoside, volatile oil, stearic acid, benzoic acid, alkaloid, vitamin B2 and C etc.
Current, many peasant households adopt cultivates glossy ganoderma by using broad-leaved weedtree to carry out cutting off the method for drying as planting matrix, but cultivation cycle is long, and output is unstable, and it is bad to turn out glossy ganoderma mouthfeel, inferior quality, easily experience insect pest and virus infections; Also have use microbial process, after cultivating fruiting kind in the medium, be seeded to mushroom room and carry out fruit body management, but the making step of medium is complicated in earlier stage, higher to environmental requirement, and need equipment investment large, be not suitable for popularizing.
Summary of the invention
Instant invention overcomes shortcoming of the prior art, provide the implantation methods of a kind of linden glossy ganoderma, the method is less by weather effect, handled easily, and mode is comparatively flexible, the glossy ganoderma cultivated not only good looking appearance, well-balanced, and less by pest infestation.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions:
An implantation methods for linden glossy ganoderma, comprises the following steps:
(1) processing of linden: select the linden that even, the tall and straight nothing of thickness is bending, cut into the cylindric linden block that length is 30 ~ 50cm, and cracking, snake, rotten linden block are removed;
(2) virus killing of linden block: linden block is put into by kuh-seng 4%, margosa 2%, golden cypress 0.7%, phoenix-tail fern 0.23%, salt 1.5%, betel nut 0.08%, Annaowan bolus 0.05%, the root bark of Chinese wolf-berry 0.028%, toxicity-eliminating paste 0.015%, Patrinia heterophylla 0.008%, in the compound of rhizome of cyrtomium 0.006% and all the other water composition, be put into together in pot, add a cover continuous heating 1.5 ~ 2.5 hours, flip heating 0.5 ~ 1 hour, water is poured out, again add water to and flood linden block face 4 ~ 6cm, add a cover heating 0.5 ~ 1 hour, between the period of heating, 3 ~ 4 times are stirred to linden block, finally take out linden block to be placed into aseptic shady and cool place and to cool, for subsequent use,
(3) preparation of Wild ganoderma bacterial classification liquid: select Wild ganoderma bacterial classification and be put in medium, put into constant-temperature shaking incubator, temperature is arranged on 35 ~ 46 DEG C, 120 ~ 140 hours, turns out Wild ganoderma bacterial classification liquid;
(4) inoculate: linden block and Wild ganoderma bacterial classification liquid are put in the turbine after sterilization and soaks, Wild ganoderma bacterial classification liquid will flood linden block face 2 ~ 3cm, the velocity of rotation of turbine is 60 ~ 80r/min, be placed on that temperature is 28 ~ 35 DEG C, humidity is carry out under the ventilation condition of 70 ~ 80%, the time is 90 ~ 100 hours;
(5) Spawn incubation: above-mentioned linden block is pulled out, be laid in and dry on frame, dry frame to be positioned in aseptic culturing room and to carry out, culturing room's temperature is 36 ~ 43 DEG C, and humidity is 65 ~ 75%, pressure is 0.75 ~ 0.8MPa, after 30 ~ 40 days, linden block is overturn, between culture period, ventilated 3 ~ 4 hours every 7 ~ 10 days, carried out moistening with atomizers spray mountain spring water to bacterial classification surface every 5 ~ 6 days, can wait for that fruit body is plucked;
(6) follow-up management: after having plucked fruit body, by linden block surface brush wash clean, putting into concentration is soak 5 ~ 6 hours in the salt solution of 0.5 ~ 0.7%, after drying in the shade, to be placed under burning sun 2 ~ 3 hours, to repeat step (2).
Further, the diameter of the described linden plate of step (1) is 35 ~ 45cm.
Further, be cut into machine cutting described in step (1), service machine is abrasive wheel cutting machine.
Further, step (3) described shaking table is rotary shaker, and rotating speed is 220 ~ 300r/min.
Further, step (3) described medium is as follows by the following method: by rice 10%, potassium dihydrogen sulfate 2%, glucose 1.6%, Cobastab
10.9%, lotus dish 0.75%, macaroni 0.23%, banana 0.08%, pithecellobium clypearia 0.004%, witchweed 0.006%, garden euphorbia herb 0.001%, alum 0.0009%, stringy stonecrop 0.0002%, climbing groundsel 0.0008 and excess water are uniformly mixed, normal temperature leaves standstill 0.5 ~ 1 hour, to put in electric cooker Steam by water bath 2 ~ 3 hours, impurity is isolated, show that filtrate is described medium, being placed on temperature is preserve under the condition of 2 ~ 5 DEG C.
Further, step (4) described turbine is steam turbine.
Further, the described mountain spring water of step (5) is killed virus through ultraviolet before using.
Compared with prior art, the invention has the beneficial effects as follows:
(1) select Wild ganoderma bacterial classification to cultivate, the healthy green non-pollution of bacterial classification can be ensured from source, do not contain artificial adding ingredient, harmless; (2) linden plate is used to cultivate, linden plate toughness is strong, and hardness is moderate, has soft feeling, be applicable to repeatedly cultivating, and verified by linden plate as the glossy ganoderma that base material is cultivated be in outward appearance or glossy ganoderma contained nutritional cost all to make base material than other materials better; (3) the present invention uses first and realizes strain inoculation on linden plate in the mode of turbine, can ensure bacterial classification energy high density, highly actively attach on linden plate, improves quantum of output.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
An implantation methods for linden glossy ganoderma, comprises the following steps:
(1) processing of linden: select the linden that even, the tall and straight nothing of thickness is bending, cut into the cylindric linden block that length is 35cm, and cracking, snake, rotten linden block are removed; ;
(2) virus killing of linden block: linden block is put into by kuh-seng 4%, margosa 2%, golden cypress 0.7%, phoenix-tail fern 0.23%, salt 1.5%, betel nut 0.08%, Annaowan bolus 0.05%, the root bark of Chinese wolf-berry 0.028%, toxicity-eliminating paste 0.015%, Patrinia heterophylla 0.008%, in the compound of rhizome of cyrtomium 0.006% and all the other water composition, be put into together in pot, add a cover continuous heating 2.5 hours, flip heats 1 hour, water is poured out, again add water to and flood linden block face 4.5cm, add a cover heating 1 hour, between the period of heating, 4 times are stirred to linden block, finally take out linden block to be placed into aseptic shady and cool place and to cool, for subsequent use,
(3) preparation of Wild ganoderma bacterial classification liquid: select Wild ganoderma bacterial classification and be put in medium, put into constant-temperature shaking incubator, temperature is arranged on 40 DEG C, 125 hours, turns out Wild ganoderma bacterial classification liquid;
(4) inoculate: linden block and Wild ganoderma bacterial classification liquid are put in the turbine after sterilization and soaks, Wild ganoderma bacterial classification liquid will flood linden block face 2.5cm, the velocity of rotation of turbine is 70r/min, be placed on that temperature is 32 DEG C, humidity is carry out under the ventilation condition of 75%, the time is 95 hours;
(5) Spawn incubation: above-mentioned linden block is pulled out, be laid in and dry on frame, dry frame to be positioned in aseptic culturing room and to carry out, culturing room's temperature is 42 DEG C, and humidity is 70%, pressure is 0.8MPa, after 30 days, linden block is overturn, between culture period, ventilated 3 hours every 8 days, carried out moistening with atomizers spray mountain spring water to bacterial classification surface every 6 days, can wait for that fruit body is plucked;
(6) follow-up management: after having plucked fruit body, by linden block surface brush wash clean, putting into concentration is soak 6 hours in the salt solution of 0.6%, after drying in the shade, is placed in burning sun lower 2 hours, repeats step (2).
Further, the diameter of the described linden plate of step (1) is 45cm.
Further, be cut into machine cutting described in step (1), service machine is abrasive wheel cutting machine.
Further, step (3) described shaking table is rotary shaker, and rotating speed is 250r/min.
Further, step (3) described medium is as follows by the following method: by rice 10%, potassium dihydrogen sulfate 2%, glucose 1.6%, Cobastab
10.9%, lotus dish 0.75%, macaroni 0.23%, banana 0.08%, pithecellobium clypearia 0.004%, witchweed 0.006%, garden euphorbia herb 0.001%, alum 0.0009%, stringy stonecrop 0.0002%, climbing groundsel 0.0008 and excess water are uniformly mixed, normal temperature leaves standstill 1 hour, to put in electric cooker Steam by water bath 2 hours, impurity is isolated, show that filtrate is described medium, being placed on temperature is preserve under the condition of 4 DEG C.
Further, step (4) described turbine is steam turbine.
Further, the described mountain spring water of step (5) is killed virus through ultraviolet before using.
Last it is noted that these are only the preferred embodiments of the present invention; be not limited to the present invention; although with reference to embodiment to invention has been detailed description; for a person skilled in the art; it still can be modified to the technical scheme described in foregoing embodiments; or equivalent replacement is carried out to wherein portion of techniques feature; but it is within the spirit and principles in the present invention all; any amendment of doing, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. an implantation methods for linden glossy ganoderma, is characterized in that, comprises the following steps:
(1) processing of linden: select the linden that even, the tall and straight nothing of thickness is bending, cut into the cylindric linden block that length is 30 ~ 50cm, and cracking, snake, rotten linden block are removed;
(2) virus killing of linden block: linden block is put into by kuh-seng 4%, margosa 2%, golden cypress 0.7%, phoenix-tail fern 0.23%, salt 1.5%, betel nut 0.08%, Annaowan bolus 0.05%, the root bark of Chinese wolf-berry 0.028%, toxicity-eliminating paste 0.015%, Patrinia heterophylla 0.008%, in the compound of rhizome of cyrtomium 0.006% and all the other water composition, be put into together in pot, add a cover continuous heating 1.5 ~ 2.5 hours, flip heating 0.5 ~ 1 hour, water is poured out, again add water to and flood linden block face 4 ~ 6cm, add a cover heating 0.5 ~ 1 hour, between the period of heating, 3 ~ 4 times are stirred to linden block, finally take out linden block to be placed into aseptic shady and cool place and to cool, for subsequent use,
(3) preparation of Wild ganoderma bacterial classification liquid: select Wild ganoderma bacterial classification and be put in medium, put into constant-temperature shaking incubator, temperature is arranged on 35 ~ 46 DEG C, 120 ~ 140 hours, turns out Wild ganoderma bacterial classification liquid;
(4) inoculate: linden block and Wild ganoderma bacterial classification liquid are put in the turbine after sterilization and soaks, Wild ganoderma bacterial classification liquid will flood linden block face 2 ~ 3cm, the velocity of rotation of turbine is 60 ~ 80r/min, be placed on that temperature is 28 ~ 35 DEG C, humidity is carry out under the ventilation condition of 70 ~ 80%, the time is 90 ~ 100 hours;
(5) Spawn incubation: above-mentioned linden block is pulled out, be laid in and dry on frame, dry frame to be positioned in aseptic culturing room and to carry out, culturing room's temperature is 36 ~ 43 DEG C, and humidity is 65 ~ 75%, pressure is 0.75 ~ 0.8MPa, after 30 ~ 40 days, linden block is overturn, between culture period, ventilated 3 ~ 4 hours every 7 ~ 10 days, carried out moistening with atomizers spray mountain spring water to bacterial classification surface every 5 ~ 6 days, can wait for that fruit body is plucked;
(6) follow-up management: after having plucked fruit body, by linden block surface brush wash clean, putting into concentration is soak 5 ~ 6 hours in the salt solution of 0.5 ~ 0.7%, after drying in the shade, to be placed under burning sun 2 ~ 3 hours, to repeat step (2).
2. the implantation methods of a kind of linden glossy ganoderma according to claim 1, is characterized in that, the diameter of the described linden plate of step (1) is 35 ~ 45cm.
3. the implantation methods of a kind of linden glossy ganoderma according to claim 1, is characterized in that, be cut into machine cutting described in step (1), service machine is abrasive wheel cutting machine.
4. the implantation methods of a kind of linden glossy ganoderma according to claim 1, is characterized in that, step (3) described shaking table is rotary shaker, and rotating speed is 220 ~ 300r/min.
5. the implantation methods of a kind of linden glossy ganoderma according to claim 1, is characterized in that, step (3) described medium is as follows by the following method: by rice 10%, potassium dihydrogen sulfate 2%, glucose 1.6%, Cobastab
10.9%, lotus dish 0.75%, macaroni 0.23%, banana 0.08%, pithecellobium clypearia 0.004%, witchweed 0.006%, garden euphorbia herb 0.001%, alum 0.0009%, stringy stonecrop 0.0002%, climbing groundsel 0.0008 and excess water are uniformly mixed, normal temperature leaves standstill 0.5 ~ 1 hour, to put in electric cooker Steam by water bath 2 ~ 3 hours, impurity is isolated, show that filtrate is described medium, being placed on temperature is preserve under the condition of 2 ~ 5 DEG C.
6. the implantation methods of a kind of linden glossy ganoderma according to claim 1, is characterized in that, step (4) described turbine is steam turbine.
7. the implantation methods of a kind of linden glossy ganoderma according to claim 1, is characterized in that, kills virus before the described mountain spring water of step (5) uses through ultraviolet.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107409758A (en) * | 2017-08-28 | 2017-12-01 | 灵川县金晨菌业有限公司 | A kind of method of cultivation basswood ganoderma lucidum |
CN107466678A (en) * | 2017-08-28 | 2017-12-15 | 灵川县金晨菌业有限公司 | A kind of method of cultivation basswood mushroom |
CN109089729A (en) * | 2018-09-07 | 2018-12-28 | 贵州高山百益食用菌发展有限公司 | A kind of making and use method of external source corncob nutrition packet |
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CN104115673A (en) * | 2014-08-08 | 2014-10-29 | 江西省农业科学院农业应用微生物研究所 | Ornamental ganoderma lucidum cultivation method |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN107409758A (en) * | 2017-08-28 | 2017-12-01 | 灵川县金晨菌业有限公司 | A kind of method of cultivation basswood ganoderma lucidum |
CN107466678A (en) * | 2017-08-28 | 2017-12-15 | 灵川县金晨菌业有限公司 | A kind of method of cultivation basswood mushroom |
CN109089729A (en) * | 2018-09-07 | 2018-12-28 | 贵州高山百益食用菌发展有限公司 | A kind of making and use method of external source corncob nutrition packet |
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Application publication date: 20151209 |