CN108310020A - A kind of preparation method of ganoderma lucidum medicine matter mycelia - Google Patents
A kind of preparation method of ganoderma lucidum medicine matter mycelia Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses a kind of preparation methods of ganoderma lucidum medicine matter mycelia, include the following steps：Medicine matter cultural hypha base is fitted into edible mushroom bag, in 120 124 DEG C of high pressure sterilizations 30 40 minutes；Cultivate lucid ganoderma stock culture；It cultivates lucid ganoderma stock culture and is inoculated with bacterium；By in ganoderma lucidum inoculation bacterium access medicine matter cultural hypha base under aseptic condition, bacterium bag is covered in culture to mycelia, collects bacteria stick, bacteria stick is dried, be crushed into powder to get.The present invention cultivates Ganoderma lucidum mycelium, not only shortens cost, improve product quality, and Chinese medicine leftover bits and pieces is made rationally to be utilized, improve resource utilization using Chinese medicine leftover bits and pieces as Medium for Ganoderma lucidum.
Description
Technical field
The present invention relates to ganoderma lucidum Cultivating techniques fields, more particularly, to a kind of preparation method of ganoderma lucidum medicine matter mycelia.
Background technology
As the fast development of the big Chinese Medicine Industry in China and the continuous propulsion of health service industry and international market are to being derived from
The favor of natural resources product promotes Chinese medicine and the production scale of natural medicinal living resources raw material constantly to expand, resource warp
Ji industrial chain is extended and is expanded.At the same time, it is discarded that a large amount of Chinese medicine is also produced in natural resources of Chinese medicinal materials industrialization process
Object is mainly derived from dis-medicinal part, medicinal material Habitat producing and the prepared slices of Chinese crude drugs of the generation of the planting of medicinal materials (cultivation) production process
The organizer official rank leftover bits and pieces discarded in production process.These general water content of Chinese medicine leftover bits and pieces are higher, and contain certain nutrition
Ingredient, it is easily corrupt, easily environment is caused seriously to pollute.At present to the processing method of traditional Chinese medicine waste：It becomes silted up and is used as waste collection
Middle stacking or burial do not only take up a large amount of soils in this way, generate largely harmful fermentation gas, and underground water easy to pollute；Yu Jiang
It is burned after traditional Chinese medicine waste drying, but the emissions such as volume of smoke, carbon dioxide is generated after burning, cause environmental pollution and resource
Significant wastage.
Ganoderma lucidum also known as celestial grass are a kind of rare Chinese medicines in China's medical treasure-house, collect health care and medicinal for all over the body.With cough-relieving
Relieving asthma, invigorating qi for tranquilization, the effect of promoting longevity.Pharmacology is proved with clinical research at all times, ganoderma lucidum truly have prevent and cure diseases, macrobiosis benefit
The effect of longevity.The Eastern Han Dynasty《Sheng Nong's herbal classic》, the Ming Dynasty famous medical scholar's Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》, all to ganoderma lucidum
Effect has detailed record extremely certainly.Present ganoderma lucidum has formally been recorded by National Pharmacopeia as drug, while it is state again
The new resource food of family's approval, has no toxic side effect, can be with medicine-food two-purpose.Although the pharmaceutical component of ganoderma lucidum is very abundant, wherein having
Effect composition can be divided into ten major class, including ganoderma lucidum polysaccharide, ganoderma lucidum polypeptide, triterpenes, 16 kinds of amino acid (wherein must containing seven kinds of human bodies
Need amino acid), protein, steroid, mannitol, coumarin glycosides, alkaloid, organic acid (main contain fumaric acid) and micro member
Plain Ge, P, Fe, Ca, Mn, Zn etc..
Based on the studies above, the present invention is directed to using Chinese medicine leftover bits and pieces as Medium for Ganoderma lucidum so that the Ganoderma Lucidum turned out
Silk has wider pharmacological action, while having pushed the recycling of Chinese medicine leftover bits and pieces, promotes natural resources of Chinese medicinal materials industry and walks
The development of circular economy, pollution-free industry.
Invention content
The purpose of the present invention is to provide a kind of preparation method of ganoderma lucidum medicine matter mycelia, this method using Chinese medicine leftover bits and pieces as
Medium for Ganoderma lucidum cultivates Ganoderma lucidum mycelium, not only shortens cost, improve product quality, and Chinese medicine leftover bits and pieces is made to be closed
Reason utilizes, and improves resource utilization.
To achieve the above object, the present invention provides the following technical solutions：
A kind of preparation method of ganoderma lucidum medicine matter mycelia, includes the following steps：
(1) medicine matter cultural hypha base is fitted into edible mushroom bag, in 120-124 DEG C of high pressure sterilization 30-40 minutes；
(2) lucid ganoderma stock culture is cultivated：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first washed with clear water
Only, it then moves into inoculating hood；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and it is thin to be cut into 2-3mm
Piece is put into 24-28 DEG C of insulating box and cultivates 5-7 days, when white hypha covers with, as lucid ganoderma stock culture；
(3) culture lucid ganoderma stock culture is inoculated with bacterium：Lucid ganoderma stock culture obtained by step (2) is inoculated into liquid fermentation medium, in
It is cultivated in 28-32 DEG C of incubator 5 days (be preferable in 30 DEG C of incubators and cultivate 5 days), obtains ganoderma lucidum and be inoculated with bacterium；
(4) ganoderma lucidum inoculation bacterium obtained by step (3) is linked into medicine matter cultural hypha base obtained by step (1) under aseptic condition
In, culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, is crushed into powder,
To obtain the final product.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, step (1) the medicine matter cultural hypha base
It is made of the raw material of following parts by weight：85-105 parts of Chinese medicine leftover bits and pieces, 5-10 parts of wheat bran, 10-12 parts of cottonseed cake, pumpkin seeds 4-7
Part, 3-5 parts of Pulvis Fellis Suis, 1-2 parts of gypsum, 1-2 parts of glucose, 110-130 parts of water.
Preferably, the preparation process of the medicine matter cultural hypha base is as follows：
(1) it takes sesame oil to set in pot, is heated to 60 DEG C -70 DEG C (being preferably heated to 65 DEG C), put into pumpkin seeds, fry to golden yellow
When, it takes out immediately, drains oil, cool, it is spare；
(2) step (1) treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine are got a foothold
Feed powder is broken, add water to stir evenly to get.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, step (3) described liquid fermentation medium
By sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:5:3:0.3:0.1:100 are formulated；System
It is for step：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in triangular flask, impregnate 12-15 hours it is (excellent
Choosing is impregnated 14 hours), then triangular flask is put into shaking table, adjustment frequency of oscillation is 220-260r/min, is fermented at 30 DEG C
100 hours, filtering, collect to get.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, step (1) is in 122 DEG C of high pressure sterilizations 35
Minute.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, step (2) is put into 26 DEG C of insulating box
Culture 6 days.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, the Chinese medicine leftover bits and pieces is Schisandra chinensis, madder
Arbitrary two kinds or more of mixture in grass, Fructus Aurantii, corn stigma, tangerine pith.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, adjustment frequency of oscillation is 240r/min.
Beneficial effects of the present invention are embodied in：The present invention is by rationally designing culture medium prescription and cultural method so that spirit
The absorption of sesame mycelia energy maximal efficiency and the active constituent being enriched in Chinese medicine leftover bits and pieces, the pharmacology to extend Ganoderma lucidum mycelium are made
With improving the quality of Ganoderma lucidum mycelium.
Specific implementation mode
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
A kind of preparation method of 1 ganoderma lucidum medicine matter mycelia of embodiment, includes the following steps：
(1) medicine matter cultural hypha base is fitted into edible mushroom bag, in 120 DEG C of high pressure sterilizations 30 minutes；
(2) lucid ganoderma stock culture is cultivated：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first washed with clear water
Only, it then moves into inoculating hood；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and it is thin to be cut into 2-3mm
Piece is put into 24 DEG C of insulating box and cultivates 5 days, when white hypha covers with, as lucid ganoderma stock culture；
(3) culture lucid ganoderma stock culture is inoculated with bacterium：Lucid ganoderma stock culture obtained by step (2) is inoculated into liquid fermentation medium, in
It is cultivated 5 days in 28 DEG C of incubators, obtains ganoderma lucidum and be inoculated with bacterium；
(4) ganoderma lucidum inoculation bacterium obtained by step (3) is linked into medicine matter cultural hypha base obtained by step (1) under aseptic condition
In, culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, is crushed into powder,
To obtain the final product.
Wherein, the medicine matter cultural hypha base is made (15g/ parts) of the raw material of following parts by weight：85 parts of Chinese medicine leftover bits and pieces,
5 parts of wheat bran, 10 parts of cottonseed cake, 4 parts of pumpkin seeds, 3 parts of Pulvis Fellis Suis, 1 part of gypsum, 1 part of glucose, 110 parts of water.
The preparation process of the medicine matter cultural hypha base is as follows：
(1) it takes sesame oil to set in pot, is heated to 60 DEG C, input pumpkin seeds take out immediately when frying to golden yellow, drain oil, put
It is cool, it is spare；
(2) step (1) treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine are got a foothold
Feed powder is broken, add water to stir evenly to get.
Wherein, the Chinese medicine leftover bits and pieces is the mixture of Schisandra chinensis and madder.
The liquid fermentation medium is by sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:
5:3:0.3:0.1:100 are formulated；Preparation process is：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in
It in triangular flask, impregnates 12 hours, then triangular flask is put into shaking table, adjustment frequency of oscillation is 220r/min, is issued in 30 DEG C
Ferment 100 hours, filtering, collect to get.
A kind of preparation method of 2 ganoderma lucidum medicine matter mycelia of embodiment, includes the following steps：
(1) medicine matter cultural hypha base is fitted into edible mushroom bag, in 124 DEG C of high pressure sterilizations 40 minutes；
(2) lucid ganoderma stock culture is cultivated：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first washed with clear water
Only, it then moves into inoculating hood；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and it is thin to be cut into 2-3mm
Piece is put into 28 DEG C of insulating box and cultivates 7 days, when white hypha covers with, as lucid ganoderma stock culture；
(3) culture lucid ganoderma stock culture is inoculated with bacterium：Lucid ganoderma stock culture obtained by step (2) is inoculated into liquid fermentation medium, in
It is cultivated 5 days in 32 DEG C of incubators, obtains ganoderma lucidum and be inoculated with bacterium；
(4) ganoderma lucidum inoculation bacterium obtained by step (3) is linked into medicine matter cultural hypha base obtained by step (1) under aseptic condition
In, culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, is crushed into powder,
To obtain the final product.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, step (1) the medicine matter cultural hypha base
It is made (35g/ parts) of the raw material of following parts by weight：105 parts of Chinese medicine leftover bits and pieces, 10 parts of wheat bran, 12 parts of cottonseed cake, pumpkin seeds 7
Part, 5 parts of Pulvis Fellis Suis, 2 parts of gypsum, 2 parts of glucose, 130 parts of water.
The preparation process of the medicine matter cultural hypha base is as follows：
(1) it takes sesame oil to set in pot, is heated to 70 DEG C, input pumpkin seeds take out immediately when frying to golden yellow, drain oil, put
It is cool, it is spare；
(2) step (1) treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine are got a foothold
Feed powder is broken, add water to stir evenly to get.
The Chinese medicine leftover bits and pieces is the mixture of Schisandra chinensis and Fructus Aurantii.
The liquid fermentation medium is by sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:
5:3:0.3:0.1:100 are formulated；Preparation process is：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in
It in triangular flask, impregnates 15 hours, then triangular flask is put into shaking table, adjustment frequency of oscillation is 260r/min, is issued in 30 DEG C
Ferment 100 hours, filtering, collect to get.
A kind of preparation method of 3 ganoderma lucidum medicine matter mycelia of embodiment, includes the following steps：
(1) medicine matter cultural hypha base is fitted into edible mushroom bag, in 121 DEG C of high pressure sterilizations 32 minutes；
(2) lucid ganoderma stock culture is cultivated：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first washed with clear water
Only, it then moves into inoculating hood；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and it is thin to be cut into 2-3mm
Piece is put into 25 DEG C of insulating box and cultivates 5 days, when white hypha covers with, as lucid ganoderma stock culture；
(3) culture lucid ganoderma stock culture is inoculated with bacterium：Lucid ganoderma stock culture obtained by step (2) is inoculated into liquid fermentation medium, in
It is cultivated 5 days in 28 DEG C of incubators, obtains ganoderma lucidum and be inoculated with bacterium；
(4) ganoderma lucidum inoculation bacterium obtained by step (3) is linked into medicine matter cultural hypha base obtained by step (1) under aseptic condition
In, culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, is crushed into powder,
To obtain the final product.
The medicine matter cultural hypha base is made (20g/ parts) of the raw material of following parts by weight：90 parts of Chinese medicine leftover bits and pieces, wheat bran
6.5 parts, 10.5 parts of cottonseed cake, 5 parts of pumpkin seeds, 3.5 parts of Pulvis Fellis Suis, 1 part of gypsum, 1 part of glucose, 115 parts of water；Medicine matter mycelia
The preparation process of culture medium is as follows：
(1) it takes sesame oil to set in pot, is heated to 60 DEG C, input pumpkin seeds take out immediately when frying to golden yellow, drain oil, put
It is cool, it is spare；
(2) step (1) treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine are got a foothold
Feed powder is broken, add water to stir evenly to get.
The Chinese medicine leftover bits and pieces is the mixture of Fructus Aurantii and corn stigma.
The liquid fermentation medium is by sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:
5:3:0.3:0.1:100 are formulated；Preparation process is：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in
It in triangular flask, impregnates 12 hours, then triangular flask is put into shaking table, adjustment frequency of oscillation is 230r/min, is issued in 30 DEG C
Ferment 100 hours, filtering, collect to get.
A kind of preparation method of 4 ganoderma lucidum medicine matter mycelia of embodiment, includes the following steps：
(1) medicine matter cultural hypha base is fitted into edible mushroom bag, in 123 DEG C of high pressure sterilizations 38 minutes；
(2) lucid ganoderma stock culture is cultivated：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first washed with clear water
Only, it then moves into inoculating hood；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and it is thin to be cut into 2-3mm
Piece is put into 28 DEG C of insulating box and cultivates 7 days, when white hypha covers with, as lucid ganoderma stock culture；
(3) culture lucid ganoderma stock culture is inoculated with bacterium：Lucid ganoderma stock culture obtained by step (2) is inoculated into liquid fermentation medium, in
It is cultivated 5 days in 32 DEG C of incubators, obtains ganoderma lucidum and be inoculated with bacterium；
(4) ganoderma lucidum inoculation bacterium obtained by step (3) is linked into medicine matter cultural hypha base obtained by step (1) under aseptic condition
In, culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, is crushed into powder,
To obtain the final product.
The medicine matter cultural hypha base is made (30g/ parts) of the raw material of following parts by weight：100 parts of Chinese medicine leftover bits and pieces, wheat bran
8 parts, 11.5 parts of cottonseed cake, 6 parts of pumpkin seeds, 4.5 parts of Pulvis Fellis Suis, 2 parts of gypsum, 2 parts of glucose, 125 parts of water；Medicine matter mycelia is trained
The preparation process for supporting base is as follows：
(1) it takes sesame oil to set in pot, is heated to 70 DEG C, input pumpkin seeds take out immediately when frying to golden yellow, drain oil, put
It is cool, it is spare；
(2) step (1) treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine are got a foothold
Feed powder is broken, add water to stir evenly to get.
The Chinese medicine leftover bits and pieces is the mixture of Schisandra chinensis and tangerine pith.
The liquid fermentation medium is by sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:
5:3:0.3:0.1:100 are formulated；Preparation process is：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in
It in triangular flask, impregnates 15 hours, then triangular flask is put into shaking table, adjustment frequency of oscillation is 250r/min, is issued in 30 DEG C
Ferment 100 hours, filtering, collect to get.
A kind of preparation method of 5 ganoderma lucidum medicine matter mycelia of embodiment, includes the following steps：
(1) medicine matter cultural hypha base is fitted into edible mushroom bag, in 12 DEG C of high pressure sterilizations 35 minutes；
(2) lucid ganoderma stock culture is cultivated：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first washed with clear water
Only, it then moves into inoculating hood；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and it is thin to be cut into 2-3mm
Piece is put into 26 DEG C of insulating box and cultivates 6 days, when white hypha covers with, as lucid ganoderma stock culture；
(3) culture lucid ganoderma stock culture is inoculated with bacterium：Lucid ganoderma stock culture obtained by step (2) is inoculated into liquid fermentation medium, in
It is cultivated 5 days in 30 DEG C of incubators, obtains ganoderma lucidum and be inoculated with bacterium；
(4) ganoderma lucidum inoculation bacterium obtained by step (3) is linked into medicine matter cultural hypha base obtained by step (1) under aseptic condition
In, culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, is crushed into powder,
To obtain the final product.
A kind of preparation method of above-mentioned ganoderma lucidum medicine matter mycelia, Preferable scheme is that, step (1) the medicine matter cultural hypha base
It is made (25g/ parts) of the raw material of following parts by weight：95 parts of Chinese medicine leftover bits and pieces, 7.5 parts of wheat bran, 11 parts of cottonseed cake, pumpkin seeds 5.5
Part, 4 parts of Pulvis Fellis Suis, 1.5 parts of gypsum, 1.5 parts of glucose, 120 parts of water；The preparation process of medicine matter cultural hypha base is as follows：
(1) it takes sesame oil to set in pot, is heated to 65 DEG C, input pumpkin seeds take out immediately when frying to golden yellow, drain oil, put
It is cool, it is spare；
(2) step (1) treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine are got a foothold
Feed powder is broken, add water to stir evenly to get.
The Chinese medicine leftover bits and pieces is the mixture of Schisandra chinensis and Fructus Aurantii.
The liquid fermentation medium is by sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:
5:3:0.3:0.1:100 are formulated；Preparation process is：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in
It in triangular flask, impregnates 14 hours, then triangular flask is put into shaking table, adjustment frequency of oscillation is 240r/min, is issued in 30 DEG C
Ferment 100 hours, filtering, collect to get.
The experimental study of test example present invention gained ganoderma lucidum medicine matter mycelia：
Subjects：Ganoderma lucidum medicine matter mycelia obtained by 1-5 of the embodiment of the present invention is experimental group；
Control group is to cultivate gained after Chinese medicine matter cultural hypha base of the present invention is replaced with common cultural hypha base in the market
Ganoderma lucidum mycelium；Wherein, other condition of culture of control group are identical with the present invention.
Test method：(1) dry mycelial weight is measured：Experimental group and control group take the bacteria stick of equivalent weight, drying to crush respectively
After fine powder, compare each group dry mycelial weight size.
(2) diameter of bacteria stick is measured：Experimental group and control group pick 50 bacteria sticks at random respectively, measure the diameter of bacteria stick,
It averages.
Test result：Mycelia weight is apparently higher than mycelia weight obtained by control group obtained by each embodiment of experimental group, and difference exists
22.1%-25.6%, difference have conspicuousness (P ＞ 0.01)；Mycelia weight compares obtained by embodiment 1-5, is not significantly different
(P ＞ 0.05).Gained bacteria stick diameter is between 25mm-28mm in experimental group, and a diameter of 20mm of bacteria stick obtained by control group；More than
Statistics indicate that present invention gained mycelia is because be enriched Chinese medicine beneficiating ingredient so that weight obviously increases, and also shows this hair indirectly
Bright gained mycelia has health-care components more, and medical value is wider；Bacteria stick diameter of the present invention is significantly greater than control group, explanation simultaneously
The formula of Chinese-medicinal culture medium is reasonable, promotes the growth of Ganoderma lucidum mycelium, improves mycelia quality.
Compare by representative example it should be pointed out that specific implementation mode is the present invention, it is clear that skill of the invention
Art scheme is not limited to the above embodiments, and can also have many variations.Those skilled in the art define public affairs with the present invention
Written description open or according to file is undoubted to be obtained, and this patent scope of the claimed is considered as.
Claims (8)
1. a kind of preparation method of ganoderma lucidum medicine matter mycelia, which is characterized in that include the following steps：
（1）Medicine matter cultural hypha base is fitted into edible mushroom bag, in 120-124 DEG C of high pressure sterilization 30-40 minutes；
（2）Cultivate lucid ganoderma stock culture：Select handle is short, meat is thick, brown-black, glossiness ganoderma lucidum as strain, first with clear water clean, so
It moves into inoculating hood afterwards；With 75% alcohol rinse, then three times with distilled water flushing, filter paper blots, and is cut into 2-3mm thin slices, is put into
It is cultivated 5-7 days in 24-28 DEG C of insulating box, when white hypha covers with, as lucid ganoderma stock culture；
（3）It cultivates lucid ganoderma stock culture and is inoculated with bacterium：By step（2）Gained lucid ganoderma stock culture is inoculated into liquid fermentation medium, in 28-32
It is cultivated 5 days in DEG C incubator（It is preferable in 30 DEG C of incubators and cultivates 5 days）, obtain ganoderma lucidum and be inoculated with bacterium；
（4）By step under aseptic condition（3）Gained ganoderma lucidum inoculation bacterium is linked into step（1）In gained medicine matter cultural hypha base, in
Culture to mycelia covers with bacterium bag under the conditions of 26 DEG C of temperature, humidity 60%, collects bacteria stick, bacteria stick is dried, be crushed into powder to get.
2. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 1, which is characterized in that step（1）The medicine
Matter cultural hypha base is made of the raw material of following parts by weight：85-105 parts of Chinese medicine leftover bits and pieces, 5-10 parts of wheat bran, cottonseed cake 10-12
Part, 4-7 parts of pumpkin seeds, 3-5 parts of Pulvis Fellis Suis, 1-2 parts of gypsum, 1-2 parts of glucose, 110-130 parts of water.
3. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 2, which is characterized in that the medicine matter mycelia training
The preparation process for supporting base is as follows：
（1）It takes sesame oil to set in pot, is heated to 60 DEG C -70 DEG C（It is preferably heated to 65 DEG C）, pumpkin seeds are put into, when frying to golden yellow,
It takes out immediately, drains oil, cool, it is spare；
（2）By step（1）Treated pumpkin seeds and wheat bran, cottonseed cake, Pulvis Fellis Suis, gypsum, glucose and Chinese medicine leftover bits and pieces feed powder
It is broken, add water to stir evenly to get.
4. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 1, which is characterized in that step（3）The liquid
Body fermentation medium is by sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water according to mass ratio 1:5:3:0.3:0.1:100
It is formulated；Preparation process is：Sucrose, dried silkworm chrysalis meal, wheat bran, magnesium sulfate, dibastic sodium phosphate and water are placed in triangular flask, impregnated
12-15 hours（It is preferred that impregnating 14 hours）, then triangular flask is put into shaking table, adjustment frequency of oscillation is 220-260r/min,
Ferment 100 hours at 30 DEG C, filter, collect to get.
5. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 1, which is characterized in that step（1）In 122 DEG C
High pressure sterilization 35 minutes.
6. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 1, which is characterized in that step（2）It is put into 26
DEG C insulating box in cultivate 6 days.
7. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 2, which is characterized in that the Chinese medicine leftover bits and pieces
For the arbitrary two kinds or more of mixture in Schisandra chinensis, madder, Fructus Aurantii, corn stigma, tangerine pith.
8. a kind of preparation method of ganoderma lucidum medicine matter mycelia according to claim 4, which is characterized in that adjusting frequency of oscillation is
240r/min。
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| CN111700218A (en) * | 2020-05-13 | 2020-09-25 | 中国农业科学院农产品加工研究所 | Corn functional fungus grain and preparation method thereof |
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