The present invention relates to a kind of production method of ganoderma lucidum mycelium, belong to mushroom culture technique field.

The cultivation of existing lucidum strain mostly is to carry out liquid state by shaking table in conical flask to cultivate 5-7 days, though this method can be turned out lucidum strain, because external environmental factor is restive, and easily microbiological contamination, and the spawn culture time is longer.

The objective of the invention is to overcome the deficiency of prior art and provide a kind of with short production cycle, cost is low, easily realizes suitability for industrialized production, and bacterial classification is difficult for the production method of the ganoderma lucidum mycelium that pollutes.

Technical scheme of the present invention is: this method is made up of the liquid deep layer fermenting of Ganoderma lucidum mycelium culture of strains, Ganoderma lucidum mycelium, the drying processing of ganoderma lucidum mycelium.Ganoderma lucidum mycelium culture of strains method is with ground rice 1～4%, sucrose 2～6%, dusty yeast 0.3～0.5%, dried silkworm chrysalis meal 0.1～0.9%, magnesium sulfate 0.1～0.2 ‰, potassium dihydrogen phosphate 0.2～0.3 ‰, all the other ratios are that the medium of inorganic salt solution is put into vessel in heating, boil and add behind the mixing in the box bottle and tighten bottleneck, under 120 ℃～125 ℃ temperature, carry out sterilization treatment, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the insulating box, under 27 ℃～29 ℃ temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours.The liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the eggplant-shape bottle and mycelium are taken out under aseptic condition in the input fermentation tank, the ratio of tank body and feed liquid is 1: 0.6～0.7, during nature chilling temperature to 29 ℃～30 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, throughput is that every cubic metre of liquid material of per minute feeds 1.0～1.3 cubic metres, the control pH value is between 5.0～6.8, fermentation process through 5～6 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation.It is with after ganoderma lucidum mycelium filtration, the centrifugation that the drying of ganoderma lucidum mycelium is handled, and carries out vacuum drying under 35 ℃～90 ℃ temperature, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.

The ganoderma lucidum mycelium that adopts said method to produce detects through Yunnan Province health and epidemic prevention station, it does not contain harmful material, carry out qualitative and quantitative analysis through Kunming Inst. of Botany, Chinese Academy of Sciences, think that ganoderma lucidum mycelium, zymotic fluid compare with natural glossy ganoderma, they have same or analogous composition, and wherein bioactive GL-B, amino acid and other nutrition are than fruit body height.

Embodiment of the invention flow chart is:

The invention will be further described below in conjunction with flow chart:

Embodiment one:

Ganoderma lucidum mycelium culture of strains method is with common ground rice 2%, sucrose 2%, dusty yeast 0.3%, dried silkworm chrysalis meal 0.1%, magnesium sulfate 0.1 ‰, potassium dihydrogen phosphate 0.2 ‰, all the other ratios are that the medium of inorganic salt solution is put into common vessel in heating, add in the eggplant-shape bottle after under 100 ℃ of temperature, boiling mixing and tighten bottleneck, on 120 ℃ of temperature, carry out sterilization treatment with conventional method, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the known insulating box, under 27 ℃ of temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours.The liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the eggplant-shape bottle and mycelium are taken out under aseptic condition in 1000 liters of fermentation tanks of input, the ratio of tank body and feed liquid is 1: 0.7, during nature chilling temperature to 29 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, the feeding amount is that every cubic metre of liquid material of per minute feeds 1 cubic metre, the control pH value is between 5.0～6.0, fermentation process through 5 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation.It is after the known plate-frame filtering of Ganoderma lucidum mycelium body and function, known centrifuge are separated, to carry out vacuum drying under 35 ℃ of temperature that the drying of ganoderma lucidum mycelium is handled, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.Glossy ganoderma dry powder yield is 8.3 ‰ under the above-mentioned condition.

Embodiment two:

Ganoderma lucidum mycelium culture of strains method is with purple rice powder 4%, dried silkworm chrysalis meal 0.9%, sucrose 6%, dusty yeast 0.5%, magnesium sulfate 0.2 ‰, potassium dihydrogen phosphate 0.3 ‰, all the other ratios are that the medium of inorganic salt solution is put into common vessel in heating, add in the eggplant-shape bottle after under 100 ℃ of temperature, boiling mixing and tighten bottleneck, on 125 ℃ of temperature, carry out sterilization treatment with conventional method, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the known insulating box, under 29 ℃ of temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours.The liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the eggplant-shape bottle and mycelium are taken out under aseptic condition in 1000 liters of fermentation tanks of input, the ratio of tank body and feed liquid is 1: 0.7, during nature chilling temperature to 30 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, the feeding amount is that every cubic metre of liquid material of per minute feeds 1.3 cubic metres, the control pH value is between 6～6.8, fermentation process through 6 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation.It is after the known plate-frame filtering of Ganoderma lucidum mycelium body and function, known centrifuge are separated, to carry out vacuum drying under 35 ℃ of temperature that the drying of ganoderma lucidum mycelium is handled, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.Glossy ganoderma dry powder yield is 8.4 ‰ under the above-mentioned condition.

The glossy ganoderma filament of above-mentioned two embodiment productions detects through Kunming Inst. of Botany, Chinese Academy of Sciences, contains 16 seed amino acids, and wherein eight kinds of necessary amino acid of human body contain seven kinds.Amino acid content is 6.07 times of fruit body in the ganoderma lucidum mycelium, and free aminoacid content is 2.19 times of fruit body.Contain 16 seed amino acids, carbohydrate in the ferment filtrate, Cobastab ₁, B ₂, its V ₀Content surpass the content of pear and other fruit, and do not contain harmful material.

The present invention compared with prior art has with short production cycle, and cost is low, and glossy ganoderma dry powder yield height, bacterial classification are difficult for polluting, and easily realize advantages such as suitability for industrialized production.