CN1099217A - Method for production of Ganoderma lucidum mycelium - Google Patents
Method for production of Ganoderma lucidum mycelium Download PDFInfo
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- CN1099217A CN1099217A CN94107140A CN94107140A CN1099217A CN 1099217 A CN1099217 A CN 1099217A CN 94107140 A CN94107140 A CN 94107140A CN 94107140 A CN94107140 A CN 94107140A CN 1099217 A CN1099217 A CN 1099217A
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- ganoderma lucidum
- lucidum mycelium
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Abstract
The culture medium of present solid culture method is composed of rice flour, silkworm chrysalis powder, cane sugar, ferment powder, magnesium sulfate and potassium dihydrogen phosphate. Glossy ganoderma hyphae are obtained by deep layer fermentation of hypha liquor, its powder is obtained through drying and crushing. The present method features short production period, low cost, high yield and not easy to be polluted hyphae.
Description
The present invention relates to a kind of production method of ganoderma lucidum mycelium, belong to mushroom culture technique field.
The cultivation of existing lucidum strain mostly is to carry out liquid state by shaking table in conical flask to cultivate 5-7 days, though this method can be turned out lucidum strain, because external environmental factor is restive, and easily microbiological contamination, and the spawn culture time is longer.
The objective of the invention is to overcome the deficiency of prior art and provide a kind of with short production cycle, cost is low, easily realizes suitability for industrialized production, and bacterial classification is difficult for the production method of the ganoderma lucidum mycelium that pollutes.
Technical scheme of the present invention is: this method is made up of the liquid deep layer fermenting of Ganoderma lucidum mycelium culture of strains, Ganoderma lucidum mycelium, the drying processing of ganoderma lucidum mycelium.Ganoderma lucidum mycelium culture of strains method is with ground rice 1～4%, sucrose 2～6%, dusty yeast 0.3～0.5%, dried silkworm chrysalis meal 0.1～0.9%, magnesium sulfate 0.1～0.2 ‰, potassium dihydrogen phosphate 0.2～0.3 ‰, all the other ratios are that the medium of inorganic salt solution is put into vessel in heating, boil and add behind the mixing in the box bottle and tighten bottleneck, under 120 ℃～125 ℃ temperature, carry out sterilization treatment, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the insulating box, under 27 ℃～29 ℃ temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours.The liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the eggplant-shape bottle and mycelium are taken out under aseptic condition in the input fermentation tank, the ratio of tank body and feed liquid is 1: 0.6～0.7, during nature chilling temperature to 29 ℃～30 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, throughput is that every cubic metre of liquid material of per minute feeds 1.0～1.3 cubic metres, the control pH value is between 5.0～6.8, fermentation process through 5～6 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation.It is with after ganoderma lucidum mycelium filtration, the centrifugation that the drying of ganoderma lucidum mycelium is handled, and carries out vacuum drying under 35 ℃～90 ℃ temperature, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.
The ganoderma lucidum mycelium that adopts said method to produce detects through Yunnan Province health and epidemic prevention station, it does not contain harmful material, carry out qualitative and quantitative analysis through Kunming Inst. of Botany, Chinese Academy of Sciences, think that ganoderma lucidum mycelium, zymotic fluid compare with natural glossy ganoderma, they have same or analogous composition, and wherein bioactive GL-B, amino acid and other nutrition are than fruit body height.
Embodiment of the invention flow chart is:
The invention will be further described below in conjunction with flow chart:
Embodiment one:
Ganoderma lucidum mycelium culture of strains method is with common ground rice 2%, sucrose 2%, dusty yeast 0.3%, dried silkworm chrysalis meal 0.1%, magnesium sulfate 0.1 ‰, potassium dihydrogen phosphate 0.2 ‰, all the other ratios are that the medium of inorganic salt solution is put into common vessel in heating, add in the eggplant-shape bottle after under 100 ℃ of temperature, boiling mixing and tighten bottleneck, on 120 ℃ of temperature, carry out sterilization treatment with conventional method, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the known insulating box, under 27 ℃ of temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours.The liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the eggplant-shape bottle and mycelium are taken out under aseptic condition in 1000 liters of fermentation tanks of input, the ratio of tank body and feed liquid is 1: 0.7, during nature chilling temperature to 29 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, the feeding amount is that every cubic metre of liquid material of per minute feeds 1 cubic metre, the control pH value is between 5.0～6.0, fermentation process through 5 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation.It is after the known plate-frame filtering of Ganoderma lucidum mycelium body and function, known centrifuge are separated, to carry out vacuum drying under 35 ℃ of temperature that the drying of ganoderma lucidum mycelium is handled, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.Glossy ganoderma dry powder yield is 8.3 ‰ under the above-mentioned condition.
Embodiment two:
Ganoderma lucidum mycelium culture of strains method is with purple rice powder 4%, dried silkworm chrysalis meal 0.9%, sucrose 6%, dusty yeast 0.5%, magnesium sulfate 0.2 ‰, potassium dihydrogen phosphate 0.3 ‰, all the other ratios are that the medium of inorganic salt solution is put into common vessel in heating, add in the eggplant-shape bottle after under 100 ℃ of temperature, boiling mixing and tighten bottleneck, on 125 ℃ of temperature, carry out sterilization treatment with conventional method, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the known insulating box, under 29 ℃ of temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours.The liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the eggplant-shape bottle and mycelium are taken out under aseptic condition in 1000 liters of fermentation tanks of input, the ratio of tank body and feed liquid is 1: 0.7, during nature chilling temperature to 30 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, the feeding amount is that every cubic metre of liquid material of per minute feeds 1.3 cubic metres, the control pH value is between 6～6.8, fermentation process through 6 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation.It is after the known plate-frame filtering of Ganoderma lucidum mycelium body and function, known centrifuge are separated, to carry out vacuum drying under 35 ℃ of temperature that the drying of ganoderma lucidum mycelium is handled, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.Glossy ganoderma dry powder yield is 8.4 ‰ under the above-mentioned condition.
The glossy ganoderma filament of above-mentioned two embodiment productions detects through Kunming Inst. of Botany, Chinese Academy of Sciences, contains 16 seed amino acids, and wherein eight kinds of necessary amino acid of human body contain seven kinds.Amino acid content is 6.07 times of fruit body in the ganoderma lucidum mycelium, and free aminoacid content is 2.19 times of fruit body.Contain 16 seed amino acids, carbohydrate in the ferment filtrate, Cobastab
1, B
2, its V
0Content surpass the content of pear and other fruit, and do not contain harmful material.
The present invention compared with prior art has with short production cycle, and cost is low, and glossy ganoderma dry powder yield height, bacterial classification are difficult for polluting, and easily realize advantages such as suitability for industrialized production.
Claims (3)
- A kind of production method of ganoderma lucidum mycelium is made up of the liquid deep layer fermenting of Ganoderma lucidum mycelium culture of strains, Ganoderma lucidum mycelium, the drying processing of ganoderma lucidum mycelium, it is characterized in that:1, Ganoderma lucidum mycelium culture of strains method is with ground rice 1～4%, dried silkworm chrysalis meal 0.1～0.9%, sucrose 2～6%, dusty yeast 0.3～0.5%, magnesium sulfate 0.1～0.2 ‰, potassium dihydrogen phosphate 0.2～0.3 ‰, all the other ratios are that the medium of inorganic salt solution is put into vessel in heating, boil and add behind the mixing in the eggplant-shape bottle and tighten bottleneck, under 120 ℃～125 ℃ temperature through sterilization treatment, naturally the cooling back is standby, under aseptic condition, inclined-plane red ganoderma bacterial strain is inserted in the bottle then, tighten to be placed on behind the bottleneck and carry out constant temperature culture in the insulating box, under 27 ℃～29 ℃ temperature, cultivate and obtained ganoderma lucidum mycelium in 96 hours;
- 2, the liquid deep layer fermenting process of Ganoderma lucidum mycelium is that medium in the box bottle and mycelium are taken out under aseptic condition, in the input fermentation tank, when the ratio of tank body and feed liquid is 1: 0.6～0.7 a natural chilling temperature to 29 ℃～30 ℃, bacterial classification is inoculated in the liquid material by the inoculation mouth under aseptic condition, begin after the sealing to stir, feed filtrated air simultaneously, throughput is that every cubic metre of liquid material of per minute feeds 1.0～1.3 cubic metres, the control pH value is between 5.0～6.8, fermentation process through 5～6 days, fermentation reaches terminal point, Ganoderma lucidum mycelium maturation;
- 3, the processing of the drying of ganoderma lucidum mycelium is with after ganoderma lucidum mycelium filtration, the centrifugation, carries out vacuum drying under 35 ℃～90 ℃ temperature, and the ganoderma lucidum mycelium after drying is handled is made glossy ganoderma dry powder more after crushed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94107140A CN1099217A (en) | 1994-06-10 | 1994-06-10 | Method for production of Ganoderma lucidum mycelium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94107140A CN1099217A (en) | 1994-06-10 | 1994-06-10 | Method for production of Ganoderma lucidum mycelium |
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| CN1099217A true CN1099217A (en) | 1995-03-01 |
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| CN94107140A Pending CN1099217A (en) | 1994-06-10 | 1994-06-10 | Method for production of Ganoderma lucidum mycelium |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077781C (en) * | 1997-12-11 | 2002-01-16 | 山西大学 | Glossy ganoderma nourishing powder and its production method |
| CN102318864A (en) * | 2011-07-27 | 2012-01-18 | 武汉百安生物科技有限责任公司 | Drink containing soy peptide and edible fungi polysaccharide and preparation method thereof |
| CN102550296A (en) * | 2012-02-15 | 2012-07-11 | 梅州市微生物研究所 | Indoor cultivation method for Ganoderma lucidum |
| CN102792849A (en) * | 2011-05-27 | 2012-11-28 | 绵阳市星光微生物研究所 | Preparation method and application of solid ganoderma lucidum mycelia |
| CN104584859A (en) * | 2015-01-01 | 2015-05-06 | 曾建斌 | Production method for soft edible lucid ganoderma |
| CN105018350A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium |
| CN105052536A (en) * | 2015-07-23 | 2015-11-18 | 广西仙草堂制药有限责任公司 | Method for cultivating Lingzhi bacterial strains via submerged fermentation |
| CN106579376A (en) * | 2016-11-30 | 2017-04-26 | 山东瑞芝生物科技有限公司 | Formula of semisolid ganoderma lucidum zymogen powder, and secondary-fermentation fully-automatic production technology |
| CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
| CN107548890A (en) * | 2017-10-24 | 2018-01-09 | 翔天农业开发集团股份有限公司 | The liquid cultivating method of mushroom |
| CN107667783A (en) * | 2017-10-24 | 2018-02-09 | 翔天农业开发集团股份有限公司 | A kind of sterile cavity method for tissue separation |
| CN108310020A (en) * | 2018-02-06 | 2018-07-24 | 青岛润达生物科技有限公司 | A kind of preparation method of ganoderma lucidum medicine matter mycelia |
| CN108575549A (en) * | 2018-03-15 | 2018-09-28 | 贵州原生态生物科技有限公司 | A kind of simulated wild glossy ganoderma cultivating superior high-yield method |
| CN109275819A (en) * | 2018-11-27 | 2019-01-29 | 广东中测食品化妆品安全评价中心有限公司 | A kind of Ganoderma lucidum submerged fermentation technology and its fermented product and application |
-
1994
- 1994-06-10 CN CN94107140A patent/CN1099217A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077781C (en) * | 1997-12-11 | 2002-01-16 | 山西大学 | Glossy ganoderma nourishing powder and its production method |
| CN102792849A (en) * | 2011-05-27 | 2012-11-28 | 绵阳市星光微生物研究所 | Preparation method and application of solid ganoderma lucidum mycelia |
| CN102318864A (en) * | 2011-07-27 | 2012-01-18 | 武汉百安生物科技有限责任公司 | Drink containing soy peptide and edible fungi polysaccharide and preparation method thereof |
| CN102318864B (en) * | 2011-07-27 | 2013-01-16 | 武汉百安生物科技有限责任公司 | Drink containing soy peptide and edible fungi polysaccharide and preparation method thereof |
| CN102550296A (en) * | 2012-02-15 | 2012-07-11 | 梅州市微生物研究所 | Indoor cultivation method for Ganoderma lucidum |
| CN105018350A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium |
| CN104584859A (en) * | 2015-01-01 | 2015-05-06 | 曾建斌 | Production method for soft edible lucid ganoderma |
| CN105052536A (en) * | 2015-07-23 | 2015-11-18 | 广西仙草堂制药有限责任公司 | Method for cultivating Lingzhi bacterial strains via submerged fermentation |
| CN106579376A (en) * | 2016-11-30 | 2017-04-26 | 山东瑞芝生物科技有限公司 | Formula of semisolid ganoderma lucidum zymogen powder, and secondary-fermentation fully-automatic production technology |
| CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
| CN107548890A (en) * | 2017-10-24 | 2018-01-09 | 翔天农业开发集团股份有限公司 | The liquid cultivating method of mushroom |
| CN107667783A (en) * | 2017-10-24 | 2018-02-09 | 翔天农业开发集团股份有限公司 | A kind of sterile cavity method for tissue separation |
| CN108310020A (en) * | 2018-02-06 | 2018-07-24 | 青岛润达生物科技有限公司 | A kind of preparation method of ganoderma lucidum medicine matter mycelia |
| CN108575549A (en) * | 2018-03-15 | 2018-09-28 | 贵州原生态生物科技有限公司 | A kind of simulated wild glossy ganoderma cultivating superior high-yield method |
| CN109275819A (en) * | 2018-11-27 | 2019-01-29 | 广东中测食品化妆品安全评价中心有限公司 | A kind of Ganoderma lucidum submerged fermentation technology and its fermented product and application |
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