CN103299823A - Method for improving yield and trace element enrichment of liquid fermentation mycelium of lucid ganoderma - Google Patents

Method for improving yield and trace element enrichment of liquid fermentation mycelium of lucid ganoderma Download PDF

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Publication number
CN103299823A
CN103299823A CN 201210073103 CN201210073103A CN103299823A CN 103299823 A CN103299823 A CN 103299823A CN 201210073103 CN201210073103 CN 201210073103 CN 201210073103 A CN201210073103 A CN 201210073103A CN 103299823 A CN103299823 A CN 103299823A
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mycelium
ganoderma lucidum
enrichment
ganoderma
product
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张佳
高虹
史德芳
蒋杰
郭永富
张建国
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HUBEI JIAFU BIOLOGICAL TECHNOLOGY Co Ltd
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HUBEI JIAFU BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The invention relates to the field of liquid fermentation mycelium of lucid ganoderma and provides a method for improving yield and trace element enrichment of the liquid fermentation mycelium of the lucid ganoderma. The method is characterized by comprising the steps of inoculating a slope lucid ganoderma strain onto a liquid seed culture medium added with trace element-containing substances, performing mutation breeding by ultraviolet rays and cobalt-60 gamma rays, placing the lucid ganoderma mycelium into a fermentation tank for expansion breeding step by step, and performing separation filtering, vacuum microwave drying and crushing on the lucid ganoderma mycelium to obtain a product. The method adopts the fermentation process controlled by adopting pH value steady state feedback step by step according to the composition and distribution of the trace elements in the culture medium, thus more facilitating improving the conversion efficiency of multiple trace elements; the process is efficient and controllable, and the mycelium is high in additional value and suitable for process production; and the drying method for the mycelium adopts hot wind combined with a vacuum microwave drying technology and keeps the biological activity of effective constituents in the mycelium.

Description

A kind of Ganoderma lucidum submerged fermentation mycelium improves the method for output and micro-enrichment
Technical field
The present invention relates to Ganoderma lucidum submerged fermentation mycelium field, is the method that a kind of Ganoderma lucidum submerged fermentation mycelium improves output and micro-enrichment.
Background technology
The consciousness of living with health of people along with developing rapidly of China's economy improves, under current allegro working method, the dysbolism that the bad habits and customs that different crowd forms cause, thereby the reduction that the trace element that causes is taken in is diseases induced, one of tracing it to its cause is exactly that people's dietary structure is unreasonable, the loss of the too meticulous nutriment that causes of edible processing mode and trace element.Therefore one of focus of Nutritional studies is exactly the meals enhanced products of developing and promoting high nutrition, non-chemically synthesize, be suitable for digesting and assimilating at present, reaches instant edible, absorbs complete, the superior dietetic purpose of quality.
Glossy ganoderma contains multiple physiologically active ingredient as a kind of fungi of dietotherapeutic, and medical value is higher, and is praised highly by China's traditional medicine always.Can supply in a large number mycelium by the liquid deep layer fermenting technology, mycelium can organically combine the trace elements such as zinc, iron, selenium, germanium simultaneously, further improve the health-care effect of tunning, the ganoderma lucidum fruitbody of comparing, be easy to digest and assimilate, production cycle is shorter, and edible quality safety is controlled, and therefore can be used as current a kind of effective technology satisfies people to the demand of ganoderma lucidum product variation and edible health.
Trace element has important physiological function, it is the growth factor that to synthesize voluntarily in the human body, quantitative proportion is not only arranged when coexisting in human body, the more important thing is that they adjusting, balance by shareds such as mutual synergies and are keeping the normal physiological metabolism of human body, excessive metallic element can cause poisoning, suppresses thalli growth.In ganoderma lucidum liquid submerged fermentation process, only has clear and definite mycelium to the different transform modes of different trace elements, by regulating addition, the control conversion ratio, realize that ganoderma lucidum mycelium forms the complex compound part by biofermentation with relevant trace element, by the impact on Ganoderma lucidum mycelium bulk-growth and trophic function of science test and reasonable assessment trace element, adopt mutagenesis acclimatization technology and interpolation trace element to transform lucidum strain, reach and improve the ganoderma lucidum mycelium biomass and strengthen ganoderma lucidum mycelium to the accumulation ability purpose of wholesome trace element.
Existing patent with the Ganoderma lucidum submerged fermentation technical licensing has " preparation method of ganoderma biological activity multivitamin intesifying agent " (Chinese patent grant number CN100379850C), take Ganoderma Lucidum as bio-carrier, at first be the preparation medium, prepare medium with carbon source, nitrogenous source, chiltern element; Next is the medium sterilization that will prepare; The 3rd is to add Liquid Strain of Ganoderma Lucidum in medium; The 4th is that certain hour is cultivated in ventilation under the uniform temperature condition, obtains liquid form product; The 5th be liquid form product is concentrated, dry, be broken and crushed into powder products.Technique of the present invention is simple, and is easy to operate, can be widely used in immunization programs for children power low, children's calcium deficiency, hypoferric anemia; Human female is weak, physiologic anemia; Prevention and the treatment of the osteoporosis that the decline of the elderly's immunity function, endocrinopathy cause; Existing patent with the Ganoderma lucidum submerged fermentation art applications has " a kind of liquid fermentation method that utilizes rare earth element to improve yield of ganoderma lucidum triterpenoid " (Chinese patent application number 201010230701.6), " production method of multielement glossy ganoderma selenium " (Chinese patent application 99108733.X), " method for producing germanium-enriched glossy ganoderma mycelium " (Chinese patent application number 200910011338.6), " ultraviolet ray irradiation improves the method for vitamin D content in the lucid ganoderma calcium fortifier " (Chinese patent application number 201010230330.1), weak point is summarized as: 1. trace element forms single; 2. do not carry out the mutagenesis domestication to having the Rich in Trace Element ability and being suitable for the liquid state fermentation ganoderma strain capable, or induced-mutation technique is single; 3. process for mycelium after the fermentation, particularly dry processing is simple, causes the mycelium solubility poor, and quality reduces, even has affected the activity of mycelium active ingredient.
Summary of the invention
The objective of the invention is to adopt ultraviolet joint Co 60 gamma ray mutagenesis domestication, screening is suitable for the ganoderma strain capable of enrichment various trace elements, through a kind of edible safety of liquid fermentation and culture, be beneficial to the ganoderma lucidum mycelium that is rich in various trace elements of absorption of human body and utilization.
Technical scheme of the present invention is:
A kind of Ganoderma lucidum submerged fermentation mycelium improves the method for output and micro-enrichment, it is characterized in that the inclined-plane lucidum strain is inoculated into liquid seed culture medium, add in the medium and contain microelement substance through ultraviolet and the domestication of Co 60 gamma ray mutagenic and breeding, enlarge step by step cultivation to fermentation tank again, through behind the liquid deep layer fermenting, ganoderma lucidum mycelium separating and filtering, vacuum microwave drying, pulverizing obtain product;
Add consisting of of trace element in its seed and the fermentation medium configuration:
Figure BSA00000686780400021
The mutagenesis domesticated strain of selecting is: lucidum strain is gold ground glossy ganoderma (state's product are recognized bacterium 2007044), river sesame No. 6 (state's product are recognized bacterium 2007045), glossy ganoderma G26 (state's product are recognized bacterium 2007046), No. 1, Ganoderma lucidum (state's product are recognized bacterium 2007047);
The method of mutagenesis of selecting is ultraviolet and the mutagenesis of Co 60 gamma ray, and the condition of ultraviolet mutagenesis is: get the seed culture medium of inoculation after 2 days, liquid level thickness is 2cm, directly over the uviol lamp of power 20W, apart from 20cm, 28 ℃ shone in continuous three days, each 10min; The Co 60 mutagenic condition is: the bacterial strain of selecting behind the ultraviolet mutagenesis of learning from else's experience, be transferred in the seed culture medium, and cultivate after 2 days, under 8KGy Co 60 gamma ray, shine 24h;
In the fermented and cultured process, the pH value has adopted control by stages, and the 3-4 days initial stage of fermented and cultured, the pH value was controlled at 5.5-6.1, adjusted to subsequently 4.0-3.3;
The dry heated-air drying combined vacuum microwave drying technology that adopts of mycelium, dividing thickness after mycelium filters is 1.5cm, at 60 ℃ of heated-air drying 2h, vacuum microwave drying then, vacuum is 25Kpa, microwave power 10KW, drying time 42min.
Owing to adopting technique scheme, the present invention is used for the glossy ganoderma liquid submerged fermentation with various trace elements, has greatly improved the output of ganoderma lucidum mycelium, and mycelium production and GL-B reach respectively 80g/L and 4.8g/L; Mycelium that the present invention produces has passed through the mutagenesis domestication, more be conducive to the chemical element that human body is difficult for absorbing is converted into biological complex state, effectively improved mycelial bioavilability, and contained micro-abundant species, higher edible and medical value had; The present invention institute forms according to trace element in the medium and distributes, and adopts the zymotechnique of pH value stable state feedback control by stages, more is conducive to improve the various trace elements transformation efficiency, and technique is efficiently controlled, and the mycelium added value is higher, is suitable for explained hereafter; Mycelial drying means adopts hot air combined vacuum microwave drying technology among the present invention, has kept the biologically active of active ingredient in the mycelium.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1
A kind of Ganoderma lucidum submerged fermentation mycelium improves the method for output and micro-enrichment, and selected mutagenesis domesticated strain is: lucidum strain is gold ground glossy ganoderma (state's product are recognized bacterium 2007044), river sesame No. 6 (state's product are recognized bacterium 2007045), glossy ganoderma G26 (state's product are recognized bacterium 2007046), No. 1, Ganoderma lucidum (state's product are recognized bacterium 2007047);
Add consisting of of trace element in the seed of carbon source, nitrogenous source and trace element preparation and the fermentation medium configuration:
Figure BSA00000686780400041
The medium sterilization that configures, 121 ℃, 30min;
To on the PDA medium slant, be inoculated into liquid seed culture medium by eugonic lucidum strain, through ultraviolet and the domestication of Co 60 mutagenic and breeding, method of mutagenesis is ultraviolet and Co 60 mutagenesis, get the seed culture medium of inoculation after 2 days, liquid level thickness is 2cm, directly over the uviol lamp of power 20W, apart from 20cm, 28 ℃ shone in continuous three days, each 10min; The Co 60 mutagenic condition is: the bacterial strain of selecting behind the ultraviolet mutagenesis of learning from else's experience, be transferred in the seed culture medium, and cultivate after 2 days, irradiation is 24 hours under 8KGy Co 60 gamma ray.According to biomass indexes, select Ganoderma lucidum and be the best bacterial classification of growth No. 1;
Ganoderma lucidum is carried out fermentation tank for No. 1 enlarge cultivation, in the fermented and cultured process, the pH value has adopted control by stages, and at 4 days initial stages of fermented and cultured, the pH value is controlled at 5.5, adjusts to subsequently 3.3;
Through behind the liquid deep layer fermenting, the ganoderma lucidum mycelium separating and filtering, the dry heated-air drying combined vacuum microwave drying technology that adopts of mycelium, dividing thickness after mycelium filters is 1.5cm, at 60 ℃ of heated-air drying 2h, then vacuum microwave dryings, vacuum is 25Kpa, microwave power 10KW, drying time 42min, crushed after being dried obtains product.
Embodiment 2
A kind of Ganoderma lucidum submerged fermentation mycelium improves the method for output and micro-enrichment, and selected mutagenesis domesticated strain is: lucidum strain is gold ground glossy ganoderma (state's product are recognized bacterium 2007044), river sesame No. 6 (state's product are recognized bacterium 2007045), glossy ganoderma G26 (state's product are recognized bacterium 2007046), No. 1, Ganoderma lucidum (state's product are recognized bacterium 2007047);
Add consisting of of trace element in the seed of carbon source, nitrogenous source and trace element preparation and the fermentation medium configuration:
Figure BSA00000686780400051
The medium sterilization that configures, 121 ℃, 30min;
To on the PDA medium slant, be inoculated into liquid seed culture medium by eugonic lucidum strain, through ultraviolet and the domestication of Co 60 mutagenic and breeding, method of mutagenesis is ultraviolet and Co 60 mutagenesis, the condition of ultraviolet mutagenesis is: get the seed culture medium of inoculation after 2 days, liquid level thickness is 2cm, directly over the uviol lamp of power 20W, apart from 20cm, 28 ℃ shone in continuous three days, each 10min; The Co 60 mutagenic condition is: the bacterial strain of selecting behind the ultraviolet mutagenesis of learning from else's experience, be transferred in the seed culture medium, and cultivate after 2 days, irradiation is 24 hours under 8KGy Co 60 gamma ray.According to biomass indexes, No. 6 (state's product are recognized bacterium 2007045) is the best bacterial classification of growth to select the river sesame;
The river sesame is occupied bacterial classification for No. 6 carry out fermentation tank expansion cultivation, in the fermented and cultured process, the pH value has adopted control by stages, and at 3 days initial stages of fermented and cultured, the pH value is controlled at 6.1, adjusts to subsequently 4.0;
Through behind the liquid deep layer fermenting, the ganoderma lucidum mycelium separating and filtering, the dry heated-air drying combined vacuum microwave drying technology that adopts of mycelium, dividing thickness after mycelium filters is 1.5cm, at 60 ℃ of heated-air drying 2h, then vacuum microwave dryings, vacuum is 25Kpa, microwave power 10KW, drying time 42min, crushed after being dried obtains product.

Claims (6)

1. a Ganoderma lucidum submerged fermentation mycelium improves the method for output and micro-enrichment, it is characterized in that the inclined-plane lucidum strain is inoculated into liquid seed culture medium, add in the medium and contain microelement substance through ultraviolet and the domestication of Co 60 gamma ray mutagenic and breeding, enlarge step by step cultivation to fermentation tank again, through behind the liquid deep layer fermenting, ganoderma lucidum mycelium separating and filtering, vacuum microwave drying, pulverizing obtain product.
2. improve the method for output and micro-enrichment according to right 1 described a kind of Ganoderma lucidum submerged fermentation mycelium, it is characterized in that, add consisting of of trace element in its seed and the fermentation medium configuration:
3. improve the method for output and micro-enrichment according to right 1 described a kind of Ganoderma lucidum submerged fermentation mycelium, it is characterized in that selected mutagenesis domesticated strain is: lucidum strain is gold ground glossy ganoderma (state's product are recognized bacterium 2007044), river sesame No. 6 (state's product are recognized bacterium 2007045), glossy ganoderma G26 (state's product are recognized bacterium 2007046), No. 1, Ganoderma lucidum (state's product are recognized bacterium 2007047).
4. improve the method for output and micro-enrichment according to right 1 described a kind of Ganoderma lucidum submerged fermentation mycelium, it is characterized in that selected method of mutagenesis is ultraviolet and the mutagenesis of Co 60 gamma ray, the condition of ultraviolet mutagenesis is: get the seed culture medium of inoculation after 2 days, liquid level thickness is 2cm, directly over the uviol lamp of power 20W, apart from 20cm, 28 ℃ shone in continuous three days, each 10min; The Co 60 mutagenic condition is: the bacterial strain of selecting behind the ultraviolet mutagenesis of learning from else's experience, be transferred in the seed culture medium, and cultivate after 2 days, under 8KGy Co 60 gamma ray, shine 24h.
5. according to the method for right 1 described a kind of Ganoderma lucidum submerged fermentation mycelium enrichment various trace elements, it is characterized in that in the fermented and cultured process, the pH value has adopted control by stages, the 3-4 days initial stage of fermented and cultured, the pH value is controlled at 5.5-6.1, adjusts to subsequently 4.0-3.3.
6. according to the method for right 1 described a kind of Ganoderma lucidum submerged fermentation mycelium enrichment various trace elements, it is characterized in that the dry heated-air drying combined vacuum microwave drying technology that adopts of mycelium, dividing thickness after mycelium filters is 1.5cm, at 60 ℃ of heated-air drying 2h, then vacuum microwave drying, vacuum is 25Kpa, microwave power 10KW, drying time 42min.
CN 201210073103 2012-03-14 2012-03-14 Method for improving yield and trace element enrichment of liquid fermentation mycelium of lucid ganoderma Pending CN103299823A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104642142A (en) * 2015-03-18 2015-05-27 云南明侍达生物科技有限责任公司 Mutation breeding method of lucid ganoderma strains
CN105009930A (en) * 2014-04-25 2015-11-04 江苏中祥高科技实业有限公司 Production method for selenium-enriched ganoderma mycelium
CN105494088A (en) * 2015-12-21 2016-04-20 镇江盛弘景观植物有限公司 Disease-resistant variety breeding method of wild ganoderma lucidum
CN105876784A (en) * 2016-03-28 2016-08-24 伊春林业科学院 Nerve-tranquilizing and heart-nourishing potassium-enriched blackcurrant capsules
CN111394414A (en) * 2020-03-25 2020-07-10 上海市农业科学院 Multi-stage liquid submerged fermentation method for lucid ganoderma

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105009930A (en) * 2014-04-25 2015-11-04 江苏中祥高科技实业有限公司 Production method for selenium-enriched ganoderma mycelium
CN105009930B (en) * 2014-04-25 2018-03-09 江苏中祥高科技实业有限公司 A kind of Se-rich lucid ganoderma mycelium production method
CN104642142A (en) * 2015-03-18 2015-05-27 云南明侍达生物科技有限责任公司 Mutation breeding method of lucid ganoderma strains
US10028480B2 (en) 2015-03-18 2018-07-24 Yunnan Mingshida-Science-Tech Co., Ltd. Ganoderma lucidum strain suitable for large-scale liquid fermentation culture, method of mutation breeding the same, and use of the strain
US10721903B2 (en) 2015-03-18 2020-07-28 Yunnan Mingshida-Science-Tech Co., Ltd. Ganoderma lucidum strain suitable for large-scale liquid fermentation culture and method of using the strain
CN105494088A (en) * 2015-12-21 2016-04-20 镇江盛弘景观植物有限公司 Disease-resistant variety breeding method of wild ganoderma lucidum
CN105876784A (en) * 2016-03-28 2016-08-24 伊春林业科学院 Nerve-tranquilizing and heart-nourishing potassium-enriched blackcurrant capsules
CN111394414A (en) * 2020-03-25 2020-07-10 上海市农业科学院 Multi-stage liquid submerged fermentation method for lucid ganoderma
CN111394414B (en) * 2020-03-25 2023-06-16 上海市农业科学院 Multistage liquid submerged fermentation method for ganoderma lucidum

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Application publication date: 20130918