CN108299030A - Purposes of the algae broken wall fermentate as foliar fertilizer - Google Patents
Purposes of the algae broken wall fermentate as foliar fertilizer Download PDFInfo
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- CN108299030A CN108299030A CN201710017554.6A CN201710017554A CN108299030A CN 108299030 A CN108299030 A CN 108299030A CN 201710017554 A CN201710017554 A CN 201710017554A CN 108299030 A CN108299030 A CN 108299030A
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- algae
- fermentate
- broken wall
- chlorella
- fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The present invention provides a kind of using algae broken wall fermentate as the purposes of foliar fertilizer, the cell wall of microalgae is hydrolyzed using the cellulose hydrolytic enzyme secreted by thalline, mildly to release nutritional ingredient and element possessed by the microalgae, collecting those ingredients becomes an algae broken wall fermentate stoste.It can be applied to crop using the algae broken wall fermentate or plant and plant, and promote the growth of its stem, leaf and/or fruit.
Description
Technical field
Fermentate after being decomposed the present invention relates to a kind of algae its cell wall, especially with respect to it is a kind of will using thalline
Purposes of the collected fermentate as foliar fertilizer after breaking microalgal cell walls.
Background technology
Under normal circumstances, either industrial crops such as Cereal, melon and fruit class, greengrocery or ornamental plant potting be
So that it is bloomed, several more, number of results are mostly high-quality, and the use of fertilizer is most common and convenient method.It is traditionally most-often used
Fertilizer belongs to chemical fertilizer in nature, but chemical fertilizer fertilizer efficiency is single, be easy to cause environmental pollution, and long-time service but will lead to soil
Earth is destroyed, and soil property is made to be no longer appropriate for crop or plant strain growth.Therefore, to keep agricultural sustainable, big quantization is not depended on
The system agricultural industry for learning fertilizer and chemical pesticide is necessary, and could so reduce the impact to environmental ecology, in addition, production
The energy and cost can reduce, but still constant rate of production can be maintained, or even improve income and even more continue the target that agricultural is pursued forever.
If agricultural production does not depend on chemical fertilizer, need to just substitute with biological fertilizer, organic fertilizer and minerals fertilizer,
But chemistry is promoted with biological fertilizer, the technology of organic fertilizer utilization ratio is then current most valued geobiont skill
Art.Earliest biological fertilizer is after peasant harvests vegetables and fruits, then cultivates with burning, and is then to utilize legume in recent years
Crop rotation is carried out with cereal crops.Biological fertilizer refers to containing active microbial body (including its resting spore), such as bacterium, very
The microbial body of the microorganisms such as bacterium, algae and its particular formulations of metabolite have the effect of supply plant nutrient, such as
Promote supply and total amount or stimulation plant growth or the absorption for promoting Plant To Nutrient and element of plant nutrient and element.
Thus, it is found that and using one containing enrich aforementioned active ingredient and easily obtain or preserve biological natural organic fertilizer material, will be
One important topic of geobiont technology development.
So-called foliar fertilizer refers to the fertilizer for being applied to plant leaf surface.It applies fertilizer compared to general soil, the more normal quilt of foliage dressing
It is used in such as crop and the symptom of nutrient dificiency occurs, because soil is too sour, too alkali, too closely knit made certain elements shortcoming, heavy rain
Nutrient conveying produced by ponding or arid is bad and leads to the production of the promotion crop of nutrient imbalance or purpose.It is said in now
It seeks precision agriculture, high quality agricultural product, environmental protection, reduce medication under energy saving trend, foliage dressing is to a certain degree
On be a fairly effective means.Substantially, adjustment plant growth can utilize the change of fertilizer component to stimulate crop from status
Auxin/hormone is secreted, and changes its script physiological action and achievees the purpose that promote growth, although using foliar fertilizer as tune
The efficiency that whole plant physiology changes is far away from directly using plant hormone, however it is to the safe of people, to plant growth
The risk of control is also smaller.In addition, using plant hormone it is especially noted that the range of its effective concentration, and this range
Often fertilizer situation is influenced in by plant nutrition itself and physiological status, climate condition and soil, if concentration is inappropriate to making
The extent of injury of object will be quite serious.Therefore, it looks for one kind safely, effectively, and is not easy the leaf having a negative impact to plant
Leaven is also that one must solve the problems, such as.In addition, if a kind of fertilizer that can be used as foliar fertilizer and general root fertilizer simultaneously can be looked for
Group ingredient more can will adjust application side for crop or the culture for planting cultivation according to the factors such as the plant characteristic and growing environment
Formula, and the growth that more its can be promoted best.
Invention content
In order to solve project above-mentioned, looking for and using the biological fertilizer of the preferable effect of a tool and safety, help peasant
Or cultivation person is planted, the growth demand of crop or plant can be allowed to be met or be promoted, while energy under suitable or less cost
It is enough to reach under the premise of environmental protection and continue agriculture target forever, realize the situation of financial gain and environment maintenance two-win, this hair
It is bright that a kind of algae broken wall fermentate is provided, and it is applied to plant and the purposes as fertilizer.The algae broken wall fermentate is
It is made by following methods：A culture in advance is added in the microalgae of one predetermined quantity and the thalline for having a predetermined ratio with the microalgae is molten
It ferments in liquid, fermentation temperature is preferably room temperature, and fermentation time is preferably 3~5 days, wherein the thalline in the phage solution
It can eccrine fiber element hydrolytic enzyme；The microalgae makes the thalline secrete the cellulose hydrolytic enzyme to hydrolyze the thin of the microalgae after being added
Cell wall；And it collects the microalgae and is formed by an algae broken wall fermentate stoste after cell wall hydrolysis.
The microalgae is added in the form of microalgae solution, and concentration in 10 liters of water bodys according to containing concentration expressed in percentage by weight
The microalgae meter of 10%-20%；The phage solution is the phage solution or concentration expressed in percentage by weight of concentration expressed in percentage by volume 1%-3%
The fungi of 0.3%-0.4%.
The volume ratio of microalgae solution and phage solution is not provided with special limitation, can be 0.5：1~1：5 range
It is interior, preferably 1：1~1：Between 3, more preferably 1：1.
In one embodiment of this invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein thalline
It can be fungi.The fungi can be Pleurotus eryngii bacterium (Pleurotus eryngii) or oyster mushroom (Pleurotus
Ostreatus), but it is not limited thereto.
In one embodiment of this invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein microalgae
Can be chlorella vulgaris (Chlorella vulgaris), original ball chlorella (Chlorella protothecoides), albumen
Core chlorella (Chlorella pyrenoidosa), oval core chlorella (Chlorella ellipsodiea), small bead
Algae (Chlorella minutissima) or Piophila casei chlorella (Chlorella protophila), it is not limited to this.
In one embodiment of this invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein algae
Broken wall fermentate can be applied to fruit crops, such as：Pawpaw, tomato, grape or banana plant, it is not limited to this.
In another aspect of the present invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein algae
Broken wall fermentate can be applied to cereal, root crop, such as：Sorghum, broad-leaved soybean (Glycine tomentella
Hayata), corn, wheat or gardening plant, such as camellia, it is not limited to this.
In another aspect of the present invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein algae
Broken wall fermentate can be applied to leaf vegetables crop, such as：Lettuce, pakchoi or mustard (scientific name：Brassica
Capitata var.Alboglabra) plant, it is not limited to this.
In another embodiment of the invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein algae
Class broken wall fermentate is the leaf surface for being applied to plant, the back side or the two.
In another embodiment of the invention, purposes of the algae broken wall fermentate as foliar fertilizer, the wherein algae
The concentration of class broken wall fermentate application is to dilute 500 times to 3000 times with the algae broken wall fermentate stoste, preferably dilutes 500
Again to 2000 times, most preferably 500 times to 1000 times.
By the present invention using algae broken wall fermentate as the purposes of foliar fertilizer, on the one hand can have by the algae rich
Rich organic compound, other active ingredients especially in amino acid or extract, provide crop or plant cultivation is optimal
Nutrient, and best absorption pattern and utilization are reached by blade face, reach and overcomes nutrient dificiency, becomes exclusive or adjustment in response to growing environment
On the one hand the purpose of growth demand is also also realized because the algae broken wall fermentate is nontoxic to people and animals, environmentally safe
Continue the target of agricultural forever.
Embodiments of the present invention described further below, following cited embodiments are to illustrate the present invention,
It is not limited to the scope of the present invention, it is any to be familiar with this those skilled in the art, without departing from the spirit and scope of the present invention, when can do
It is a little to change and retouch, therefore protection scope of the present invention should be defined by the scope of the appended claims.
Description of the drawings
Fig. 1 is the comparison photo that the embodiment of the present invention is applied to pawpaw to its stem, leaf growth effect.
Fig. 2 is the comparison photo that the embodiment of the present invention is applied to camellia to its stem, leaf growth effect.
Fig. 3 is the comparison photo that the embodiment of the present invention is applied to that holy girl's tomato influences its fruit growth.
Fig. 4 is the comparison photo that the embodiment of the present invention is applied to black nylon dish to its stem, leaf growth effect.
Fig. 5 is the comparison photo that the embodiment of the present invention is applied to pakchoi to its stem, leaf growth effect.
Fig. 6 is the comparison photo that the embodiment of the present invention is applied to mustard to its stem, leaf growth effect.
Fig. 7 is the comparison photo that the embodiment of the present invention is applied to white mustard to its stem, leaf growth effect.
Specific implementation mode
A kind of purposes of the algae broken wall fermentate provided by the present invention as foliar fertilizer carries out algae broken wall fermentation first
The preparation of object.
The preparation of 1 algae broken wall fermentate of embodiment
The preparation of algae broken wall fermentate prepares mushroom solution and algae solution respectively first.What the present embodiment was utilized
The Pleurotus eryngii bacterium (Pleurotus eryngii) of mushroom category Mycophyta.Commercially available Pleurotus eryngii bacterium is cut into about 5mm pros with knife
Shape bulk is inoculated on solid medium (15g/L malt extract powders, 20g/L glucose, 6g/L yeast extract and 15g/L fine jades
Fat), it cultivates about 7 days.Aforementioned agar medium is dug out later, is added spare after 250mL sterile waters are homogenized；About algae
Class part, the present embodiment are then to take the chlorella powder (Chlorella vulgaris) of 0.5g that 10mL (weight ratios about 1 are added:20)
In sterile water, stir evenly rear spare.Pleurotus eryngii bacterium solution after taking aforementioned 10mL to homogenize, is added in the liquid culture solution of 80mL
(15g/L malt extract powders, 20g/L glucose and 6g/L yeast extract), adds 10mL green alga solution, ferments later in 50L
In bucket, at room temperature (25~35 DEG C), anaerobic fermentation is carried out about 3 days, you can obtain algae broken wall fermentate stoste.If must be with dry
Dry state is taken, then can be dried this stoste with 70 DEG C.
Preparation of the present invention about algae broken wall fermentate is using the cellulose hydrolytic enzyme secreted by thalline to algae
Cell wall is hydrolyzed, and compared to the mechanical system of grinding or cutting, or utilizes freezing repeatedly, the physics side of ultrasonic vibrating
Method or the chemical method permeated using the decomposition of special ferment, organic solvent utilize the cellulose of biological naturally secret of the invention
Hydrolytic enzyme, more can be more complete and retain all the components that the algae includes while high efficiency decomposes cell wall, and
Effect caused by its content can be made full use of.
Green alga in aforementioned utilized chlorella powder is also known as chlorella, Chlorococcum, is a kind of microalgae, is grown in fresh, nothing
The waters of pollution, belongs to one-celled plants, and cell size about only has 2~8 μm.Green alga has the two big features of plant：It is green with leaf
Body and cell wall, contain chlorophyll a and b, phylloerythrin, lutein etc. in chloroplaset, and its photosynthetic mode and high
Plant is identical.Green alga is dividing Class Xue Shang platymisciums circle (Kingdom Plantae), Chlorophyta (Division
Chlorophyta), Chlorococcum guiding principle (Class Chlorophyceae), Chlorococcale (OrderChlorocaccales), green ball
The Chlorococcum (GeneraChlorella) of algae section (Family Chlorellaceae).Available Chlorococcum is except aforementioned general
Lead to chlorella (Chlorella vulgaris) outside, still includes：Original ball chlorella (Chlorella protothecoides), egg
It is white nucleus chlorella (Chlorella pyrenoidosa), oval core chlorella (Chlorella ellipsodiea), small small
Ball algae (Chlorella minutissima) or Piophila casei chlorella (Chlorella protophila) etc., it is not limited to
This.
Aforementioned cellulose hydrolytic enzyme can will not have deliquescent cellulose decomposition into monosaccharide.Those ferment may include but
It is not limited to：Endo-type cellulose fibre amylase (endo- β -1,4-glucanase) E.C.3.2.1.4 (also known as β -1,4-D-
Glucanhydrolase or carboxymethylcellulase (CMCase)), circumscribed-type cellulose fibre amylase
(exo- β -1,4-glucanase) E.C.3.2.1.91 (also known as β -1,4-glucancellobiohydrolase or
Cellulose- β -1,4-glucancellobiosidase), the sweet ferment of β-glucose (β -1,4-glucosidase)
E.C.3.2.1.21 (also known as β -1,4-glucohydrolase or cellobiase).
Aforementioned cellulose hydrolytic enzyme can be secreted by different thalline, such as oyster mushroom (Pleurotus
Ostreatus hydrolyzed carboxymethylcellulo, e (Carboxymethylcellulose, CMC) can) be secreted.
Aforementioned Pleurotus eryngii bacterium is only to illustrate, and also can be the oyster mushroom (Pleurotus ostreatus) of Mycophyta, or
Other can secrete the bacterial strain of the aforementioned ferment for being hydrolyzed alga cells wall.
The volume ratio of aforementioned microalgae solution and phage solution is not provided with special limitation, can be 0.5：1~1：5 model
In enclosing, preferably 1：1~1：Between 3, more preferably 1：1.The concentration of the microalgae solution in 10 liters of water bodys according to containing weight
The microalgae meter of percentage concentration 10%-20%；The phage solution is the phage solution or weight of concentration expressed in percentage by volume 1%-3%
The fungi of percentage concentration 0.3%-0.4%.
2 foliar fertilizer of embodiment applies test-pawpaw
40 plants of the selection pawpaws strain of platform agriculture two is divided into control group and is tested in solarium with experimental group.Control group uses one
As chemical fertilizer (commercially available instant compound fertilizer 10-20-20).It takes 10 kilograms of fertilizer to be diluted with 10 tons of water, that is, dilutes 1000 times,
It is applied fertilizer with root reperfusion mode.Administration period is to be perfused 1 time for every 5~10 days, and (carrying out 2 months altogether) applies spill 6 times altogether.It is real
The algae broken wall fermentate stoste that group then takes 200mL as prepared by embodiment 1 is tested, is diluted with 200 liters of water, and dilution 1000
Times, and algae broken wall fermentate stostes of the 200mL as prepared by embodiment 1 separately is taken, it is diluted, that is, diluted with 400 liters of water
It 2000 times, is applied fertilizer in a manner of foliage spray respectively.Administration period is every 5~10 days 1 time (carrying out 2 months altogether), applies spill 6 altogether
It is secondary.It is noted down within the 2nd, 4 week from after the 1st fertilising, observation growth point (bud point) spacing is bloomed and result status, result
As shown in table 1 and Fig. 1.
Table 1
By the result of table 1 and Fig. 1 it is recognized that while the increased ratio of experimental group bud point is not so good as control group, but with regard to number of results
For mesh, the ratio of number of results is significant to be higher than control group.The obvious algae broken wall fermenation raw liquid using the present invention is as blade face
Fertilizer with promote pawpaw as a result, than using general chemical fertilizer effect it is more significant.
3 foliar fertilizer of embodiment applies test-camellia
It takes 700 7 inch camellia seedlings to be divided into control group to be tested with experimental group.Control group uses general chemical fertilizer (city
Sell instant compound fertilizer 10-20-20).It takes 10 kilograms of fertilizer to be diluted with 10 tons of water, that is, 1000 times is diluted, with soil-perfusion side
Formula is applied fertilizer.Administration period is that 1 time (between June~November) monthly is perfused, totally 6 times.Experimental group then takes 200ml such as embodiments 1
Prepared algae broken wall fermentate stoste is diluted with 200 liters of water, that is, is diluted 1000 times, carried out in a manner of foliage spray
Fertilising.Administration period is to apply within every 15 days to spill 1 time (carrying out 2 months altogether), applies spill 4 times altogether.It records within the 60th day from after the 1st fertilising
Record, the upgrowth situations such as observation leaf, stem, result is as shown in table 2 and Fig. 2.
Table 2
By the result of table 2 and Fig. 2 it is found that in the form part of leaf, the leaf color of control group and experimental group can all present dark green
Color, but the blade face area of experimental group is larger, and also tender shoots, compared to the only several control groups having, experimental group every is almost
There are tender shoots, whole growing state good.In terms of Stem nematode, control group does not have notable difference at the beginning, until the 45th day
Just there are apparent growth, 75 centimeters of the long average out to of branch, 1.1 centimeters of stem diameter average out to；As for control group, limb growth rate is not
Have stagnation behavior, from apply spill after just gradually grow up, 95 centimeters of the long average out to of branch, 1.4 centimeters of stem diameter average out to.Therefore, most
Whole strain camellia weight afterwards, 2.3 kilograms of experimental group average out to, in comparison, control group only has 1.8 kilograms, has lacked about 27% fully
Weight.From the above results, it can be seen that, by the method for administration of the present invention, in particular with algae broken wall fermentate as foliar fertilizer
Land use systems, can effectively generate really growth promote effect.Compared to past known algae only be used as nutriment,
Promote the health foods such as immunocompetence (Jiang Shanzong please be join to wait, cellulose hydrolytic enzyme in the application study of green alga industry,
2006, agricultural raw the 7th phase of skill industry quarterly) for, the present invention provides one completely different from known effectiveness.
4 foliar fertilizer of embodiment applies test-holy girl's tomato
Control group is divided to be tested with experimental group Two Areas with 300 level ground gardens.Control group uses general chemical fertilizer (city
Sell instant compound fertilizer 10-20-20).Take 1 kilogram of fertilizer to be diluted with 1 ton of water, that is, dilute 1000 times in a manner of soil-perfusion into
Row fertilising.Administration period is monthly to be perfused 1 time, totally 6 times.Experimental group then takes algae broken wall hairs of the 200ml as prepared by embodiment 1
Ferment object stoste is diluted with 200 liters of water, that is, dilutes 1000 times, in plant sprout after three weeks fruiting period when, with foliage spray side
Formula is applied fertilizer.Administration period is to apply within every 15 days to spill 1 time (carrying out 2 months altogether), applies spill 4 times altogether.From the 60th day after the 1st fertilising
It is noted down, the upgrowth situations such as observation leaf, stem, result such as table 3 is as shown in figure 3.
Table 3
By the result of table 3 and Fig. 3 it is found that in fruit morphology part, the fruit of control group more it is thin it is flat, it is not of uniform size and
Gloss dimness is partially yellow, but experimental group is in comparison, and fruit is quite full, the more uniform consistent and color of size is ruddy.In addition, just
Fruit sensory test, control group is more tender, moisture is less, but the fruit of experimental group is crisp compared with Q because full, and washiness, whole
Body fruit quality is excellent.On the other hand, control group number of results is more for yield, and total harvest is only 280 kilograms, and experimental group is
350 kilograms, improve yield about 25%.In addition, the sugariness of experimental group also greatly improves 4 degree.From the above results, it can be seen that, by this hair
Bright method of administration, the Land use systems in particular with algae broken wall fermentate as foliar fertilizer can effectively facilitate fruit really
Real growth, and quality, the sugariness made it have, are equally completely different from known new effectiveness.
5 foliar fertilizer of embodiment applies test-corn
Control group is divided to be tested with experimental group Two Areas with 2 hectares of fields.Control group uses general chemical fertilizer (city
Sell instant compound fertilizer 10-20-20), it takes 10 kilograms of fertilizer to be diluted with 10 tons of water, that is, 1000 times is diluted, with foliage spray side
Formula is applied fertilizer.Administration period is to apply within every 10 days to spill 1 time (carrying out 6 months altogether), applies spill 18 times altogether.Experimental group is strictly according to the facts using 10L
Apply the algae broken wall fermenation raw liquid prepared by example 1, diluted with 10 tonnes of water, that is, dilute 1000 times, in a manner of foliage spray into
Row fertilising.Administration period is to apply within every 10 days to spill 1 time (carrying out 6 months altogether), applies spill 18 times altogether.Growth situation is as shown in table 4.
Table 4
As shown in Table 4, the Stem nematode of experimental group plant obtains more sturdy, weightening up to 77% per stem, and beautiful per stem
Grain of rice number also has up to 69% growth.Therefore, the corn output per hectare of experimental group greatly improved 45%.It is obvious to utilize this
The algae broken wall fermenation raw liquid of invention is more significant by the effect than general chemical fertilizer as fertilizer purposes.
6 foliar fertilizer of embodiment applies test-wheat
Control group is divided to be tested with experimental group Two Areas with 2 hectares of fields.Control group uses general chemical fertilizer (city
Sell instant compound fertilizer 10-20-20), it takes 10 kilograms of fertilizer to be diluted with 10 tons of water, that is, 1000 times is diluted, with foliage spray side
Formula is applied fertilizer.Administration period is to apply within every 10 days to spill 1 time (carrying out 4 months altogether), applies spill 12 times altogether.Experimental group is strictly according to the facts using 10L
Apply the algae broken wall fermenation raw liquid prepared by example 1, diluted with 10 tonnes of water, that is, dilute 1000 times, in a manner of foliage spray into
Row fertilising.Administration period is to apply within every 10 days to spill 1 time (carrying out 4 months altogether), applies spill 12 times altogether.Growth situation is as shown in table 5.
Table 5
As shown in Table 5, the grain ear that experimental group wheat plant is tied is more full, and paddy galassing increases 40%, and whole
Yield is greatly improved to 140%, almost the 2.5 of script times.The obvious algae broken wall fermenation raw liquid using the present invention is as fertilizer
Expect purposes, the effect than general chemical fertilizer is more significant.
7 foliar fertilizer of embodiment applies test-grape
Control group is divided to be tested with experimental group Two Areas with 2 hectares of fields.Control group uses general chemical fertilizer (city
Sell instant compound fertilizer 10-20-20), it takes 10 kilograms of fertilizer to be diluted with 10 tons of water, that is, 1000 times is diluted, with foliage spray side
Formula is applied fertilizer.Administration period is to apply within every 10 days to spill 1 time (carrying out 7 months altogether), applies spill 21 times altogether.Experimental group is strictly according to the facts using 10L
Apply the algae broken wall fermenation raw liquid prepared by example 1, diluted with 10 tonnes of water, that is, dilute 1000 times, in a manner of foliage spray into
Row fertilising.Administration period is to apply within every 10 days to spill 1 time (carrying out 7 months altogether), applies spill 21 times altogether.Growth situation is as shown in table 6.
Table 6
As shown in Table 6, each tendril of experimental group grapevine seedling equally grows more sturdy and length, every tendril increase weight
30%, and the yield of per hectare seems that the growth to tendril is related, also increases about 25%.Importantly, the sugariness of grape by
14 degree of script are greatly improved to 19 degree.It therefore, will be than one using the algae broken wall fermenation raw liquid of the present invention as fertilizer purposes
As chemical fertilizer effect it is more significant, sugariness, the quality of fruit are also obviously improved.
Application test-black nylon dish of 8 foliar fertilizer of embodiment
Control group is divided to be tested with experimental group Two Areas with 300 square centimeters of fields.Control group uses general chemistry
Fertilizer (commercially available instant compound fertilizer 10-20-20), takes 50 milliliters of fertilizer to be diluted with 25 literss of water, that is, 500 times is diluted, with leaf
Face spray pattern applies fertilizer.Administration period is to apply within every 7 days to spill 1 time (carrying out 1 month altogether), applies spill 4 times altogether.Test group uses 50
Algae broken wall fermenation raw liquid (liquid) of the milliliter as prepared by embodiment 1 is diluted with 25 liters of water, that is, 500 times is diluted, with leaf
Face spray pattern applies fertilizer.Administration period is to apply within every 7 days to spill 1 time (carrying out 1 month altogether), applies spill 4 times altogether, growth result is such as
Shown in table 7 and Fig. 4.
Table 7
By table 7 and Fig. 4 it is found that in black nylon dish experimental group, the build of each plant is more strong using general fertilizers than script
Big many, no matter leaf length, leaf width, stem length, stem thickness both increase about 15%~60%, and lobe numbers also increase 50%, make
Whole yield greatly improves.In addition, the moth in observation dish strain, it has been found that the part of experimental group reduces 7%, therefore, profit
With the algae broken wall fermenation raw liquid of the present invention as fertilizer purposes, other than more significant than the effect of general chemical fertilizer,
Improve the resistance of plant pair moth.
9 foliar fertilizer of embodiment applies test-pakchoi
Control group is divided to be tested with experimental group Two Areas with 300 square centimeters of fields.Control group uses general chemistry
Fertilizer (commercially available instant compound fertilizer 10-20-20), takes 50 milliliters of fertilizer to be diluted with 25 literss of water, that is, 500 times is diluted, with leaf
Face spray pattern applies fertilizer.Administration period is to apply within every 7 days to spill 1 time (carrying out 1 month altogether), applies spill 4 times altogether.Experimental group uses 50
Algae broken wall fermenation raw liquid (liquid) of the milliliter as prepared by embodiment 1 is diluted with 25 liters of water, that is, 500 times is diluted, with leaf
Face spray pattern applies fertilizer.Administration period is to apply within every 7 days to spill 1 time (carrying out 1 month altogether), applies spill 4 times altogether.Growth situation is such as
Shown in table 8 and Fig. 5.
Table 8
By table 8 and Fig. 5 it is found that in pakchoi experimental group, the build of each plant is grown than script using general fertilizers
Many, no matter leaf length, leaf width, stem length, stem thickness both increase about 20%~58%, and lobe numbers also increase 57%, make whole
The yield of body greatly improves.In addition, the moth in observation dish strain, it has been found that the part of experimental group reduces 5%, therefore, utilizes
The algae broken wall fermenation raw liquid of the present invention other than more significant than the effect of general chemical fertilizer, is also carried as fertilizer purposes
The high resistance of plant pair moth.
10 foliar fertilizer of embodiment applies test-mustard
Control group is divided to be tested with experimental group Two Areas with 300 square centimeters of fields.Control group uses general chemistry
Fertilizer (commercially available instant compound fertilizer 10-20-20), takes 50 milliliters of fertilizer to be diluted with 25 literss of water, that is, 500 times is diluted, with leaf
Face spray pattern applies fertilizer.Administration period is to apply within every 7 days to spill 1 time (carrying out 1 month altogether), applies spill 4 times altogether.Test group uses 50
Algae broken wall fermenation raw liquid (liquid) of the milliliter as prepared by embodiment 1 is diluted with 25 liters of water, that is, 500 times is diluted, with leaf
Face spray pattern applies fertilizer.Administration period is to apply within every 7 days to spill 1 time (carrying out 1 month altogether), applies spill 4 times altogether, growth result is such as
Shown in table 9 and Fig. 6.
Table 9
By table 9 and Fig. 6 it is found that in mustard experimental group, the build of each plant is grown than script using general fertilizers
Many, no matter leaf length, leaf width, stem length, stem thickness both increase about 28%~50%, and lobe numbers also increase 33%, make whole
The yield of body greatly improves.In addition, the moth in observation dish strain, it has been found that the part of experimental group reduces 3%, therefore, utilizes
The algae broken wall fermenation raw liquid of the present invention other than more significant than the effect of general chemical fertilizer, is also carried as fertilizer purposes
The high resistance of plant pair moth.
Application test-white mustard of 11 foliar fertilizer of embodiment
Control group is divided to be tested with experimental group Two Areas with 300 square centimeters of fields.Control group uses general chemistry
Fertilizer takes 50 milliliters of fertilizer to be diluted with 25 literss of water, that is, dilutes 500 times, applied fertilizer in a manner of foliage spray.Administration period
It was applied for every 7 days and spills 1 time (carrying out 1 month altogether), applied spill 4 times altogether.Test group is broken using 50 milliliters of algae as prepared by embodiment 1
Wall fermenation raw liquid (liquid) is diluted with 25 liters of water, that is, is diluted 500 times, applied fertilizer in a manner of foliage spray.Administration period
It was applied for every 7 days and spills 1 time (carrying out 1 month altogether), applied spill 4 times altogether, growth result is as shown in table 10 and Fig. 7.
Table 10
By table 10 and Fig. 7 it is found that the white mustard of experimental group, leaf length, leaf width, stem length, stem thickness both increase about 13%
~22%, though increasing degree is smaller, but compares and grow many using general fertilizers originally.In addition, lobe numbers but increase
75%, thus it is speculated that being the increase of the number of blade causes the size increasing degree of each blade to reduce.On the other hand, the moth in dish strain is observed
Worm, it has been found that the part of experimental group is equally reduced, and reduces 2%, therefore, utilizes the algae broken wall fermenation raw liquid of the present invention
As fertilizer purposes, other than better than general chemical fertilizer, the resistance of plant pair moth is also improved.
By above-mentioned experiment it is found that purposes of the present invention using algae broken wall fermentate as foliar fertilizer, in addition to root, stem,
In the growth regulating of leaf and fruit, generate outside the effect for promoting differentiation and development, it also can be by other contained by the fermentate
Active ingredient provides nutrient or element needed for plant strain growth, thus more can further promote the development of each organ of plant at
It is long, simultaneously because the algae broken wall fermentate has no plant itself negative growth effect, therefore in the control of concentration
Also compared with the plant injury that need not be worried caused by its imbalance.
Claims (9)
1. a kind of purposes of algae broken wall fermentate as foliar fertilizer, wherein the algae broken wall fermentate is by following methods system
：
Culture in advance is added in the microalgae of predetermined quantity and is had with the microalgae in the phage solution of predetermined ratio and is fermented,
In, the thalline in the phage solution can eccrine fiber element hydrolytic enzyme；
The microalgae makes the thalline secrete the cell wall of the cellulose hydrolytic enzyme to hydrolyze the microalgae after being added；And to collect this micro-
Algae is formed by algae broken wall fermentate stoste after cell wall hydrolysis.
2. purposes of the algae broken wall fermentate according to claim 1 as foliar fertilizer, which is characterized in that the thalline is
Fungi.
3. purposes of the algae broken wall fermentate according to claim 2 as foliar fertilizer, which is characterized in that the fungi is
Pleurotus eryngii bacterium (Pleurotus eryngii) or oyster mushroom (Pleurotus ostreatus).
4. purposes of the algae broken wall fermentate according to claim 1 as foliar fertilizer, which is characterized in that the microalgae is
Chlorella vulgaris (Chlorella vulgaris), original ball chlorella (Chlorella protothecoides), pyrenoids are small
Ball algae (Chlorella pyrenoidosa), oval core chlorella (Chlorella ellipsodiea), small chlorella
(Chlorella minutissima) or Piophila casei chlorella (Chlorella protophila).
5. purposes of the algae broken wall fermentate according to claim 1 as foliar fertilizer, which is characterized in that the algae is broken
Wall fermentate is to be applied to pawpaw, tomato, grape or banana plant.
6. purposes of the algae broken wall fermentate according to claim 1 as foliar fertilizer, which is characterized in that the algae is broken
Wall fermentate is to be applied to sorghum, broad-leaved soybean, corn, camellia or wheat plant.
7. purposes of the algae broken wall fermentate according to claim 1 as foliar fertilizer, which is characterized in that the algae is broken
Wall fermentate is to be applied to lettuce, pakchoi or mustard plant.
8. purposes of the algae broken wall fermentate according to claim 1,5,6 or 7 as foliar fertilizer, which is characterized in that institute
It is the leaf surface for being applied to plant, the back side or the two to state algae broken wall fermentate.
9. purposes of the algae broken wall fermentate according to claim 1 as foliar fertilizer, which is characterized in that the algae is broken
The concentration of wall fermentate application is to dilute 500 times to 3000 times with the algae broken wall fermentate stoste.
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| CN201710017554.6A CN108299030A (en) | 2017-01-11 | 2017-01-11 | Purposes of the algae broken wall fermentate as foliar fertilizer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710017554.6A CN108299030A (en) | 2017-01-11 | 2017-01-11 | Purposes of the algae broken wall fermentate as foliar fertilizer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112159825A (en) * | 2020-09-25 | 2021-01-01 | 广州友方生物科技有限公司 | Method for extracting DHA (docosahexaenoic acid) by fermentation |
| EP4052577A1 (en) * | 2021-03-05 | 2022-09-07 | AlgiMatter B.V. | Use of microalgae |
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| CN102060578A (en) * | 2010-11-15 | 2011-05-18 | 北京科技大学 | Method for producing microecological foliar fertilizer by utilizing probiotics and microalgae |
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| CN112159825A (en) * | 2020-09-25 | 2021-01-01 | 广州友方生物科技有限公司 | Method for extracting DHA (docosahexaenoic acid) by fermentation |
| EP4052577A1 (en) * | 2021-03-05 | 2022-09-07 | AlgiMatter B.V. | Use of microalgae |
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Application publication date: 20180720 |