WO2022088284A1 - Method of preparing silkworm chrysalis-cultured cordyceps militaris tablet - Google Patents
Method of preparing silkworm chrysalis-cultured cordyceps militaris tablet Download PDFInfo
- Publication number
- WO2022088284A1 WO2022088284A1 PCT/CN2020/129039 CN2020129039W WO2022088284A1 WO 2022088284 A1 WO2022088284 A1 WO 2022088284A1 CN 2020129039 W CN2020129039 W CN 2020129039W WO 2022088284 A1 WO2022088284 A1 WO 2022088284A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- cordyceps militaris
- silkworm
- silkworm chrysalis
- drying
- tablet
- Prior art date
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 87
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 15
- 239000008187 granular material Substances 0.000 claims abstract description 14
- 238000005469 granulation Methods 0.000 claims abstract description 11
- 230000003179 granulation Effects 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000010298 pulverizing process Methods 0.000 claims abstract description 8
- 239000007779 soft material Substances 0.000 claims abstract description 8
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- 241000190633 Cordyceps Species 0.000 claims description 20
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 229910052709 silver Inorganic materials 0.000 claims description 2
- 239000004332 silver Substances 0.000 claims description 2
- 239000004480 active ingredient Substances 0.000 abstract description 10
- 238000005507 spraying Methods 0.000 abstract 1
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 9
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 9
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 9
- 150000004676 glycans Chemical class 0.000 description 6
- 229920001282 polysaccharide Polymers 0.000 description 6
- 239000005017 polysaccharide Substances 0.000 description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 5
- FPVKHBSQESCIEP-UHFFFAOYSA-N (8S)-3-(2-deoxy-beta-D-erythro-pentofuranosyl)-3,6,7,8-tetrahydroimidazo[4,5-d][1,3]diazepin-8-ol Natural products C1C(O)C(CO)OC1N1C(NC=NCC2O)=C2N=C1 FPVKHBSQESCIEP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000209504 Poaceae Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- FPVKHBSQESCIEP-JQCXWYLXSA-N pentostatin Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC[C@H]2O)=C2N=C1 FPVKHBSQESCIEP-JQCXWYLXSA-N 0.000 description 2
- 229960002340 pentostatin Drugs 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
Definitions
- the invention relates to the technical field of cordyceps, in particular to a preparation method of silkworm cordyceps tablets.
- Silkworm Cordyceps militaris is a combination of insects and fungi which is artificially cultivated by inoculating the spores of wild natural Cordyceps militaris strains on silkworm chrysalis.
- Cordyceps militaris contains more cordycepin, cordycepic acid and SOD than natural cordyceps, northern cordyceps and silkworm cordyceps. Therefore, cordyceps militaris is a kind of cordyceps that is comparable to wild cordyceps.
- Silkworm Cordyceps militaris can be made into silkworm Cordyceps militaris tablets by post-processing, but the existing preparation method of silkworm Cordyceps militaris tablets has problems such as high cost and low content of active ingredients of silkworm Cordyceps militaris, and needs to be improved.
- the purpose of the present invention is to provide a preparation method of silkworm cordyceps militaris tablets, the preparation cost is low, and the silkworm cordyceps militaris tablet contains high active ingredient content of silkworm cordyceps militaris.
- the technical scheme of the present invention is to design a preparation method of Cordyceps militaris tablet, comprising the following steps:
- step 2) pulverizing: ultrafine pulverization is carried out to the silkworm cordyceps militaris cultivated in step 1) to obtain silkworm cordyceps militaris powder;
- step 5 Granulation: spray distilled water on the silkworm cordyceps militaris powder after the ultraviolet sterilization treatment in step 4), make silkworm cordyceps militaris soft material from silkworm cordyceps militaris powder, and then pour the silkworm cordyceps militaris soft material into a wet granulator and shake granulation to obtain Silkworm Cordyceps granules;
- step 6 After drying and processing the Cordyceps militaris granules obtained in step 5), add them to a tableting machine for tableting to obtain Cordyceps militaris tablets.
- step 1) the fresh silkworm chrysalis is first pierced with a silver needle for several times, then the pierced silkworm chrysalis is cleaned and sterilized, and then inoculated, dark cultured and light cultured.
- the temperature of the drying treatment is controlled at 25-30°C, and the drying treatment time is 2.5-4.5 hours.
- the ultraviolet sterilization treatment time is 30-35 minutes.
- step 5 an 85-90 mesh screen is used during the swing granulation.
- the temperature of the drying treatment is controlled at 45-50° C., and the water content of the granules of Cordyceps militaris is controlled below 4%.
- the advantages and beneficial effects of the present invention are as follows: a preparation method of the silkworm cordyceps militaris tablet is provided, the preparation cost is low, and the active ingredient content of the silkworm cordyceps militaris tablet is high.
- the method firstly uses silver needles to pierce the fresh silkworm chrysalis several times, and then cleans and sterilizes the pierced silkworm chrysalis, thereby ensuring and improving the cleaning and sterilizing treatment effect of the silkworm chrysalis, and laying a good foundation for the subsequent cultivation of the silkworm chrysalis.
- the present invention pierces the silkworm chrysalis multiple times, which can shorten the time for the emergence of Cordyceps militaris, and at the same time, can increase the amount of grasses produced, and can also increase the amount of cordycepin, cordycepic acid, Cordyceps polysaccharide, SOD enzyme, pentostatin, etc. in the silkworm Cordyceps militaris. Active ingredient content.
- the present invention adopts distilled water to granulate, has low cost, and is free from pollution in the whole process of treating silkworm Cordyceps militaris.
- the finally obtained silkworm Cordyceps militaris tablet contains various active ingredients such as cordycepin, cordycepin acid, cordyceps polysaccharide, etc., and the content of active ingredients is high .
- the content of cordycepin is not less than 0.8wt%
- the content of cordycepic acid is not less than 2.4wt%
- the content of Cordyceps polysaccharide is not less than 6wt%.
- the invention provides a preparation method of silkworm cordyceps tablet, comprising the following steps:
- the temperature of the drying treatment is controlled at 25 to 30°C, and the drying treatment time is 2.5 to 4.5 hours;
- the time of UV sterilization treatment is 30 to 35 minutes;
- step 5 Granulation: spray distilled water on the silkworm cordyceps militaris powder after the ultraviolet sterilization treatment in step 4), make silkworm cordyceps militaris soft material from silkworm cordyceps militaris powder, and then pour the silkworm cordyceps militaris soft material into a wet granulator and shake granulation to obtain Silkworm Cordyceps granules;
- Tablet compression add the Cordyceps militaris granules obtained in step 5) after drying treatment and add them to a tablet press to perform tablet compression to obtain Cordyceps militaris tablets;
- the temperature of the drying treatment is controlled at 45-50° C., and the water content of the Cordyceps militaris granules is controlled below 4%.
- the method firstly uses silver needles to pierce the fresh silkworm chrysalis multiple times, and then cleans and sterilizes the pierced silkworm chrysalis, thereby ensuring and improving the cleaning and sterilizing treatment effect of the silkworm chrysalis, and laying a good foundation for the subsequent cultivation of the silkworm chrysalis.
- the present invention pierces the silkworm chrysalis for many times, which can shorten the time of emergence of Cordyceps militaris, and at the same time can increase the amount of grasses produced, and can also increase the amount of cordycepin, cordycepic acid, Cordyceps polysaccharide, SOD enzyme, pentostatin, etc. in the silkworm Cordyceps militaris. Active ingredient content.
- the present invention adopts distilled water to granulate, has low cost, and is free from pollution in the whole process of treating silkworm Cordyceps militaris.
- the finally obtained silkworm Cordyceps militaris tablet contains various active ingredients such as cordycepin, cordycepin acid, cordyceps polysaccharide, etc., and the content of active ingredients is high .
- the content of cordycepin is not less than 0.8wt%
- the content of cordycepic acid is not less than 2.4wt%
- the content of Cordyceps polysaccharide is not less than 6wt%.
Abstract
A method of preparing a silkworm chrysalis-cultured Cordyceps militaris tablet, comprising the following steps: 1) cultivation: cultivating silkworm chrysalis-cultured Cordyceps militaris using fresh silkworm chrysalis; 2) pulverization: superfinely pulverizing the silkworm chrysalis-cultured Cordyceps militaris cultivated in step 1) to obtain a silkworm chrysalis-cultured Cordyceps militaris powder; 3) drying: drying the powder obtained in step 2); 4) sterilization: performing ultraviolet sterilization on the powder obtained in step 3); 5) granulation: spraying the powder which has undergone the ultraviolet sterilization in step 4) with distilled water to prepare same into a soft material, and then pouring the soft material into a wet granulator and performing shaking granulation to obtain granules; and 6) tableting: drying the granules obtained in step 5) and then adding the dried granules to a tableting machine and performing tableting to obtain a silkworm chrysalis-cultured Cordyceps militaris tablet. The method of preparing the silkworm chrysalis-cultured Cordyceps militaris tablet has low preparation costs, and the tablet has a high content of silkworm chrysalis-cultured Cordyceps militaris active ingredients.
Description
本发明涉及虫草技术领域，具体涉及一种蚕蛹虫草片剂的制备方法。The invention relates to the technical field of cordyceps, in particular to a preparation method of silkworm cordyceps tablets.
蚕蛹虫草是用野生天然蛹虫草菌株孢子接种到蚕蛹上人工培育而成的虫菌结合体。蚕蛹虫草内含的虫草素、虫草酸、SOD等超过天然冬虫夏草、北虫草和榨蚕虫草，故蚕蛹虫草是一种能与野生冬虫夏草相媲美的虫草。Silkworm Cordyceps militaris is a combination of insects and fungi which is artificially cultivated by inoculating the spores of wild natural Cordyceps militaris strains on silkworm chrysalis. Cordyceps militaris contains more cordycepin, cordycepic acid and SOD than natural cordyceps, northern cordyceps and silkworm cordyceps. Therefore, cordyceps militaris is a kind of cordyceps that is comparable to wild cordyceps.
蚕蛹虫草可通过后期处理制作出蚕蛹虫草片剂，但现有的蚕蛹虫草片剂的制备方法，存在成本高、蚕蛹虫草有效成分含量底等问题，有待改进。Silkworm Cordyceps militaris can be made into silkworm Cordyceps militaris tablets by post-processing, but the existing preparation method of silkworm Cordyceps militaris tablets has problems such as high cost and low content of active ingredients of silkworm Cordyceps militaris, and needs to be improved.
本发明的目的在于提供一种蚕蛹虫草片剂的制备方法，其制备成本低，且蚕蛹虫草片剂中蚕蛹虫草有效成分含量高。The purpose of the present invention is to provide a preparation method of silkworm cordyceps militaris tablets, the preparation cost is low, and the silkworm cordyceps militaris tablet contains high active ingredient content of silkworm cordyceps militaris.
为实现上述目的，本发明的技术方案是设计一种蚕蛹虫草片剂的制备方法，包括如下步骤：In order to achieve the above object, the technical scheme of the present invention is to design a preparation method of Cordyceps militaris tablet, comprising the following steps:
1）培养：以鲜活的蚕蛹培养出蚕蛹虫草；1) Cultivation: Cultivate silkworm chrysalis with fresh silkworm chrysalis;
2）粉碎：对步骤1）培养出的蚕蛹虫草进行超微粉碎，得到蚕蛹虫草粉；2) pulverizing: ultrafine pulverization is carried out to the silkworm cordyceps militaris cultivated in step 1) to obtain silkworm cordyceps militaris powder;
3）烘干：对步骤2）得到的蚕蛹虫草粉进行烘干处理；3) Drying: drying the silkworm Cordyceps militaris powder obtained in step 2);
4）灭菌：对步骤3）烘干处理后的蚕蛹虫草粉进行紫外灭菌处理；4) Sterilization: UV sterilization is performed on the silkworm Cordyceps militaris powder after drying in step 3);
5）造粒：向步骤4）紫外灭菌处理后的蚕蛹虫草粉喷洒蒸馏水，将蚕蛹虫草粉制成蚕蛹虫草软材，再将蚕蛹虫草软材倒入湿法制粒机中摇摆制粒，得到蚕蛹虫草颗粒；5) Granulation: spray distilled water on the silkworm cordyceps militaris powder after the ultraviolet sterilization treatment in step 4), make silkworm cordyceps militaris soft material from silkworm cordyceps militaris powder, and then pour the silkworm cordyceps militaris soft material into a wet granulator and shake granulation to obtain Silkworm Cordyceps granules;
6）压片：将步骤5）得到的蚕蛹虫草颗粒烘干处理后加入压片机中进行压片，得到蚕蛹虫草片剂。6) Tableting: After drying and processing the Cordyceps militaris granules obtained in step 5), add them to a tableting machine for tableting to obtain Cordyceps militaris tablets.
优选的，步骤1）中，先用银针将鲜活的蚕蛹刺穿多次，再对刺穿后的蚕蛹进行清洗灭菌，再进行接种、暗培养和光培养。Preferably, in step 1), the fresh silkworm chrysalis is first pierced with a silver needle for several times, then the pierced silkworm chrysalis is cleaned and sterilized, and then inoculated, dark cultured and light cultured.
优选的，步骤3）中，烘干处理的温度控制在25～30℃，烘干处理的时间为2.5～4.5小时。Preferably, in step 3), the temperature of the drying treatment is controlled at 25-30°C, and the drying treatment time is 2.5-4.5 hours.
优选的，步骤4）中，紫外灭菌处理的时间为30～35分钟。Preferably, in step 4), the ultraviolet sterilization treatment time is 30-35 minutes.
优选的，步骤5）中，摇摆制粒时采用85～90目的筛网。Preferably, in step 5), an 85-90 mesh screen is used during the swing granulation.
优选的，步骤6）中，烘干处理的温度控制在45～50℃，且使蚕蛹虫草颗粒的含水量控制在4％以下。Preferably, in step 6), the temperature of the drying treatment is controlled at 45-50° C., and the water content of the granules of Cordyceps militaris is controlled below 4%.
本发明的优点和有益效果在于：提供一种蚕蛹虫草片剂的制备方法，其制备成本低，且蚕蛹虫草片剂中蚕蛹虫草有效成分含量高。The advantages and beneficial effects of the present invention are as follows: a preparation method of the silkworm cordyceps militaris tablet is provided, the preparation cost is low, and the active ingredient content of the silkworm cordyceps militaris tablet is high.
本发明先用银针将鲜活的蚕蛹刺穿多次，再对刺穿后的蚕蛹进行清洗灭菌，可保证和提高蚕蛹的清洗灭菌处理效果，给后续蚕蛹虫草的培养打好基础。The method firstly uses silver needles to pierce the fresh silkworm chrysalis several times, and then cleans and sterilizes the pierced silkworm chrysalis, thereby ensuring and improving the cleaning and sterilizing treatment effect of the silkworm chrysalis, and laying a good foundation for the subsequent cultivation of the silkworm chrysalis.
且本发明将蚕蛹刺穿多次，可缩短蚕蛹虫草的出草时间，同时也能增加出草量，还能提高蚕蛹虫草中虫草素、虫草酸、虫草多糖、SOD酶、喷司他丁等有效成分的含量。In addition, the present invention pierces the silkworm chrysalis multiple times, which can shorten the time for the emergence of Cordyceps militaris, and at the same time, can increase the amount of grasses produced, and can also increase the amount of cordycepin, cordycepic acid, Cordyceps polysaccharide, SOD enzyme, pentostatin, etc. in the silkworm Cordyceps militaris. Active ingredient content.
本发明采用蒸馏水造粒，成本低，且对蚕蛹虫草的处理过程中全程无污染，最终得到的蚕蛹虫草片剂，含有虫草素、虫草酸、虫草多糖等多种有效成分，且有效成分含量高。The present invention adopts distilled water to granulate, has low cost, and is free from pollution in the whole process of treating silkworm Cordyceps militaris. The finally obtained silkworm Cordyceps militaris tablet contains various active ingredients such as cordycepin, cordycepin acid, cordyceps polysaccharide, etc., and the content of active ingredients is high .
本发明蚕蛹虫草片剂中：In the silkworm cordyceps tablet of the present invention:
虫草素的含量不低于0.8wt%，The content of cordycepin is not less than 0.8wt%,
虫草酸的含量不低于2.4wt%，The content of cordycepic acid is not less than 2.4wt%,
虫草多糖的含量不低于6wt%。The content of Cordyceps polysaccharide is not less than 6wt%.
下面结合实施例，对本发明的具体实施方式作进一步描述。以下实施例仅用于更加清楚地说明本发明的技术方案，而不能以此来限制本发明的保护范围。The specific embodiments of the present invention will be further described below with reference to the examples. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and cannot be used to limit the protection scope of the present invention.
本发明提供一种蚕蛹虫草片剂的制备方法，包括如下步骤：The invention provides a preparation method of silkworm cordyceps tablet, comprising the following steps:
1）培养：以鲜活的蚕蛹培养出蚕蛹虫草；1) Cultivation: Cultivate silkworm chrysalis with fresh silkworm chrysalis;
先用银针将鲜活的蚕蛹刺穿多次，再对刺穿后的蚕蛹进行清洗灭菌，再进行接种、暗培养和光培养；First pierce the fresh silkworm chrysalis with silver needles for many times, then clean and sterilize the pierced silkworm chrysalis, and then carry out inoculation, dark culture and light culture;
2）粉碎：对步骤1）培养出的蚕蛹虫草进行超微粉碎，得到蚕蛹虫草粉；2) pulverization: ultrafine pulverization is carried out to the silkworm cordyceps militaris cultivated in step 1) to obtain silkworm cordyceps militaris powder;
3）烘干：对步骤2）得到的蚕蛹虫草粉进行烘干处理；3) Drying: drying the silkworm Cordyceps militaris powder obtained in step 2);
烘干处理的温度控制在25～30℃，烘干处理的时间为2.5～4.5小时；The temperature of the drying treatment is controlled at 25 to 30°C, and the drying treatment time is 2.5 to 4.5 hours;
4）灭菌：对步骤3）烘干处理后的蚕蛹虫草粉进行紫外灭菌处理；4) Sterilization: UV sterilization is performed on the silkworm Cordyceps militaris powder after drying in step 3);
紫外灭菌处理的时间为30～35分钟；The time of UV sterilization treatment is 30 to 35 minutes;
5）造粒：向步骤4）紫外灭菌处理后的蚕蛹虫草粉喷洒蒸馏水，将蚕蛹虫草粉制成蚕蛹虫草软材，再将蚕蛹虫草软材倒入湿法制粒机中摇摆制粒，得到蚕蛹虫草颗粒；5) Granulation: spray distilled water on the silkworm cordyceps militaris powder after the ultraviolet sterilization treatment in step 4), make silkworm cordyceps militaris soft material from silkworm cordyceps militaris powder, and then pour the silkworm cordyceps militaris soft material into a wet granulator and shake granulation to obtain Silkworm Cordyceps granules;
摇摆制粒时采用85～90目的筛网；85-90 mesh screen is used in the swing granulation;
6）压片：将步骤5）得到的蚕蛹虫草颗粒烘干处理后加入压片机中进行压片，得到蚕蛹虫草片剂；6) Tablet compression: add the Cordyceps militaris granules obtained in step 5) after drying treatment and add them to a tablet press to perform tablet compression to obtain Cordyceps militaris tablets;
烘干处理的温度控制在45～50℃，且使蚕蛹虫草颗粒的含水量控制在4％以下。The temperature of the drying treatment is controlled at 45-50° C., and the water content of the Cordyceps militaris granules is controlled below 4%.
本发明先用银针将鲜活的蚕蛹刺穿多次，再对刺穿后的蚕蛹进行清洗灭菌，可保证和提高蚕蛹的清洗灭菌处理效果，给后续蚕蛹虫草的培养打好基础。The method firstly uses silver needles to pierce the fresh silkworm chrysalis multiple times, and then cleans and sterilizes the pierced silkworm chrysalis, thereby ensuring and improving the cleaning and sterilizing treatment effect of the silkworm chrysalis, and laying a good foundation for the subsequent cultivation of the silkworm chrysalis.
且本发明将蚕蛹刺穿多次，可缩短蚕蛹虫草的出草时间，同时也能增加出草量，还能提高蚕蛹虫草中虫草素、虫草酸、虫草多糖、SOD酶、喷司他丁等有效成分的含量。In addition, the present invention pierces the silkworm chrysalis for many times, which can shorten the time of emergence of Cordyceps militaris, and at the same time can increase the amount of grasses produced, and can also increase the amount of cordycepin, cordycepic acid, Cordyceps polysaccharide, SOD enzyme, pentostatin, etc. in the silkworm Cordyceps militaris. Active ingredient content.
本发明采用蒸馏水造粒，成本低，且对蚕蛹虫草的处理过程中全程无污染，最终得到的蚕蛹虫草片剂，含有虫草素、虫草酸、虫草多糖等多种有效成分，且有效成分含量高。The present invention adopts distilled water to granulate, has low cost, and is free from pollution in the whole process of treating silkworm Cordyceps militaris. The finally obtained silkworm Cordyceps militaris tablet contains various active ingredients such as cordycepin, cordycepin acid, cordyceps polysaccharide, etc., and the content of active ingredients is high .
本发明蚕蛹虫草片剂中：In the silkworm cordyceps tablet of the present invention:
虫草素的含量不低于0.8wt%，The content of cordycepin is not less than 0.8wt%,
虫草酸的含量不低于2.4wt%，The content of cordycepic acid is not less than 2.4wt%,
虫草多糖的含量不低于6wt%。The content of Cordyceps polysaccharide is not less than 6wt%.
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明技术原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。The above are only the preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the technical principles of the present invention, several improvements and modifications can be made. These improvements and modifications It should also be regarded as the protection scope of the present invention.
Claims (6)
- 一种蚕蛹虫草片剂的制备方法，其特征在于，包括如下步骤：A kind of preparation method of silkworm cordyceps tablet, is characterized in that, comprises the steps:1）培养：以鲜活的蚕蛹培养出蚕蛹虫草；1) Cultivation: Cultivate silkworm chrysalis with fresh silkworm chrysalis;2）粉碎：对步骤1）培养出的蚕蛹虫草进行超微粉碎，得到蚕蛹虫草粉；2) pulverization: ultrafine pulverization is carried out to the silkworm cordyceps militaris cultivated in step 1) to obtain silkworm cordyceps militaris powder;3）烘干：对步骤2）得到的蚕蛹虫草粉进行烘干处理；3) Drying: drying the silkworm Cordyceps militaris powder obtained in step 2);4）灭菌：对步骤3）烘干处理后的蚕蛹虫草粉进行紫外灭菌处理；4) Sterilization: UV sterilization is performed on the silkworm Cordyceps militaris powder after drying in step 3);5）造粒：向步骤4）紫外灭菌处理后的蚕蛹虫草粉喷洒蒸馏水，将蚕蛹虫草粉制成蚕蛹虫草软材，再将蚕蛹虫草软材倒入湿法制粒机中摇摆制粒，得到蚕蛹虫草颗粒；5) Granulation: spray distilled water on the silkworm cordyceps militaris powder after the ultraviolet sterilization treatment in step 4), make silkworm cordyceps militaris soft material from silkworm cordyceps militaris powder, and then pour the silkworm cordyceps militaris soft material into a wet granulator and shake granulation to obtain Silkworm Cordyceps granules;6）压片：将步骤5）得到的蚕蛹虫草颗粒烘干处理后加入压片机中进行压片，得到蚕蛹虫草片剂。6) Tableting: After drying and processing the Cordyceps militaris granules obtained in step 5), add them to a tableting machine for tableting to obtain Cordyceps militaris tablets.
- 根据权利要求1所述的蚕蛹虫草片剂的制备方法，其特征在于，步骤1）中，先用银针将鲜活的蚕蛹刺穿多次，再对刺穿后的蚕蛹进行清洗灭菌，再进行接种、暗培养和光培养。The preparation method of silkworm chrysalis tablet according to claim 1, is characterized in that, in step 1), first use silver needle to pierce the fresh silkworm chrysalis multiple times, and then clean and sterilize the pierced silkworm chrysalis, Inoculation, dark culture and light culture were performed again.
- 根据权利要求2所述的蚕蛹虫草片剂的制备方法，其特征在于，步骤3）中，烘干处理的温度控制在25～30℃，烘干处理的时间为2.5～4.5小时。The preparation method of silkworm Cordyceps militaris tablet according to claim 2, characterized in that, in step 3), the temperature of the drying treatment is controlled at 25-30°C, and the drying treatment time is 2.5-4.5 hours.
- 根据权利要求3所述的蚕蛹虫草片剂的制备方法，其特征在于，步骤4）中，紫外灭菌处理的时间为30～35分钟。The preparation method of silkworm cordyceps militaris tablet according to claim 3, characterized in that, in step 4), the time of ultraviolet sterilization treatment is 30-35 minutes.
- 根据权利要求4所述的蚕蛹虫草片剂的制备方法，其特征在于，步骤5）中，摇摆制粒时采用85～90目的筛网。The preparation method of the silkworm Cordyceps militaris tablet according to claim 4, characterized in that, in step 5), 85-90 mesh sieves are used during swing granulation.
- 根据权利要求5所述的蚕蛹虫草片剂的制备方法，其特征在于，步骤6）中，烘干处理的温度控制在45～50℃，且使蚕蛹虫草颗粒的含水量控制在4％以下。The preparation method of silkworm cordyceps militaris tablets according to claim 5, characterized in that, in step 6), the temperature of the drying treatment is controlled at 45-50° C., and the water content of the silkworm cordyceps militaris granules is controlled below 4%.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011160769.1 | 2020-10-27 | ||
| CN202011160769.1A CN112190602A (en) | 2020-10-27 | 2020-10-27 | Preparation method of silkworm chrysalis cordyceps sinensis tablets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2022088284A1 true WO2022088284A1 (en) | 2022-05-05 |
Family
ID=74011467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2020/129039 WO2022088284A1 (en) | 2020-10-27 | 2020-11-16 | Method of preparing silkworm chrysalis-cultured cordyceps militaris tablet |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN112190602A (en) |
| WO (1) | WO2022088284A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114963700A (en) * | 2022-05-26 | 2022-08-30 | 四川默森药业有限公司 | Dried silkworm chrysalis cordyceps sinensis and preparation process thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114870040A (en) * | 2022-05-13 | 2022-08-09 | 苏州家和蚕业生物科技有限公司 | Sterilization method of cordyceps militaris |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515920A (en) * | 2011-12-23 | 2012-06-27 | 正源堂（天津）生物科技有限公司 | Culture of cordyceps militaris and preparation method of oral cordyceps militaris tablet |
| CN103652923A (en) * | 2013-12-11 | 2014-03-26 | 黄秀英 | Preparation method of cordyceps sinensis lozenge |
| CN104381996A (en) * | 2014-11-19 | 2015-03-04 | 苏州俞氏药业有限公司 | Cordyceps militaris tablet and preparation method thereof |
| CN104585733A (en) * | 2014-12-31 | 2015-05-06 | 台州康晶生物科技有限公司 | Cordyceps sinensis oral tablet and preparation method thereof |
| CN104605334A (en) * | 2014-12-31 | 2015-05-13 | 浙江理工大学 | Cordyceps sinensis oral tablet and preparation method thereof |
| CN104705642A (en) * | 2014-12-31 | 2015-06-17 | 浙江理工大学 | Cordyceps militaris oral tablet and preparation method thereof |
| CN206228621U (en) * | 2016-08-28 | 2017-06-09 | 吉安市御美丽健康产业股份有限公司 | A kind of pelletizing press sheet equipment for Cordyceps militaris piece |
| KR20180118413A (en) * | 2017-04-21 | 2018-10-31 | 주식회사머쉬텍 | Composition comprising Cordycepin as an effective ingredient for preventing or treating of Liver cancer and Method for preparing Butanol fraction of Cordyceps militaris |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091772A (en) * | 1993-03-06 | 1994-09-07 | 冯建忠 | The method of using baked silkworm chrysalis for artificially breeding Chinese caterpillar fungus |
| CN101429483A (en) * | 2008-12-16 | 2009-05-13 | 张洪新 | Cultivation method for silkworm chrysalis cordyceps sinensis and capsule thereof |
| CN102037856B (en) * | 2009-10-16 | 2012-06-20 | 北京农业生物技术研究中心 | Simple cordyceps militaris strain rejuvenation method |
| CN103430776B (en) * | 2013-09-04 | 2014-12-10 | 鲁东大学 | Silkworm pupa-worm grass culture method capable of improving yield and quality |
| CN103688759A (en) * | 2013-12-20 | 2014-04-02 | 凌中鑫 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
| CN104303835A (en) * | 2014-09-29 | 2015-01-28 | 苏州家和农业生物科技研究中心 | Cultivation method of cordyceps sinensis |
| CN105902577A (en) * | 2016-04-10 | 2016-08-31 | 台州康晶生物科技有限公司 | Low-sugar Cordyceps militaris oral tablet and preparation method thereof |
| CN106810366A (en) * | 2017-01-24 | 2017-06-09 | 北京新创青龙湖种植专业合作社 | A kind of cultural method for improving cordycepin content in Cordyceps militaris mycelia and silkworm chrysalis Cordyceps sinensis |
| CN107409746A (en) * | 2017-05-02 | 2017-12-01 | 徐安义 | A kind of breeding method of silkworm chrysalis Cordyceps sinensis |
| CN111758486B (en) * | 2020-07-13 | 2021-11-23 | 江西菌草生物科技有限公司 | Large-scale high-yield production and cultivation method for early-maturing cordyceps militaris of tussah |
| CN112772282B (en) * | 2021-03-11 | 2022-12-02 | 齐齐哈尔大学 | Cultivation method of cordyceps militaris |
-
2020
- 2020-10-27 CN CN202011160769.1A patent/CN112190602A/en active Pending
- 2020-11-16 WO PCT/CN2020/129039 patent/WO2022088284A1/en active Application Filing
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102515920A (en) * | 2011-12-23 | 2012-06-27 | 正源堂（天津）生物科技有限公司 | Culture of cordyceps militaris and preparation method of oral cordyceps militaris tablet |
| CN103652923A (en) * | 2013-12-11 | 2014-03-26 | 黄秀英 | Preparation method of cordyceps sinensis lozenge |
| CN104381996A (en) * | 2014-11-19 | 2015-03-04 | 苏州俞氏药业有限公司 | Cordyceps militaris tablet and preparation method thereof |
| CN104585733A (en) * | 2014-12-31 | 2015-05-06 | 台州康晶生物科技有限公司 | Cordyceps sinensis oral tablet and preparation method thereof |
| CN104605334A (en) * | 2014-12-31 | 2015-05-13 | 浙江理工大学 | Cordyceps sinensis oral tablet and preparation method thereof |
| CN104705642A (en) * | 2014-12-31 | 2015-06-17 | 浙江理工大学 | Cordyceps militaris oral tablet and preparation method thereof |
| CN206228621U (en) * | 2016-08-28 | 2017-06-09 | 吉安市御美丽健康产业股份有限公司 | A kind of pelletizing press sheet equipment for Cordyceps militaris piece |
| KR20180118413A (en) * | 2017-04-21 | 2018-10-31 | 주식회사머쉬텍 | Composition comprising Cordycepin as an effective ingredient for preventing or treating of Liver cancer and Method for preparing Butanol fraction of Cordyceps militaris |
Non-Patent Citations (1)
| Title |
|---|
| LAN ZHANG, DAN WANG, JING－SHI NIE: "Effect of Cordyceps militaris on humoral immunity and cellular immunity", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, vol. 37, no. 5, 31 March 2016 (2016-03-31), XP055924854 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114963700A (en) * | 2022-05-26 | 2022-08-30 | 四川默森药业有限公司 | Dried silkworm chrysalis cordyceps sinensis and preparation process thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112190602A (en) | 2021-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1669440A (en) | Plant insects protecting agent | |
| WO2022088284A1 (en) | Method of preparing silkworm chrysalis-cultured cordyceps militaris tablet | |
| CN104892086B (en) | A kind of compound seaweed fertilizer and preparation method thereof | |
| CN103518608B (en) | Anoectochilus formosanus seedling cultivation medium and seedling cultivation method | |
| CN107396753A (en) | A kind of cultivation technique of Silicon-rich flat mushroom | |
| CN103408335B (en) | Microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation | |
| CN104744160A (en) | Biocompound fertilizer for preventing and controlling sclerotinia and application method thereof | |
| CN104694395B (en) | One plant of Metarhizium anisopliae and its zymotechnique to peanut rot disease preventive treatment with preventive effect | |
| CN114027097A (en) | Mushroom culture medium containing nut green seedcase and preparation method thereof | |
| CN104221526B (en) | A kind of application process of soil activating agent | |
| CN109937975B (en) | Standardized earthworm breeding method | |
| CN107417409A (en) | A kind of deadwood leaf preparation method of organic fertilizer of the long lichens of energy | |
| CN106867947A (en) | A kind of method for preparing insecticide through microbial fermentation as raw material with China tree | |
| CN109526551A (en) | A kind of cultural method of natural selenium-rich agaricus bisporus | |
| CN108689759A (en) | Organic-biological stimulin and its preparation application method | |
| CN108184906A (en) | A kind of preparation method of attractive insecticide for fruit fly | |
| CN111893076A (en) | Microbial insecticide for preventing and treating aphids and preparation method thereof | |
| CN110301292A (en) | A kind of low-carbon compound selenium-enriched edible mushroom compost and its implantation methods | |
| CN110476703A (en) | A kind of cultural method for reducing edible mushroom pest and disease damage and occurring | |
| CN1632101A (en) | Method for preparing medicine-fertilizer double purpose soil microbial community trichoderm-virida preparation | |
| CN109879678A (en) | A kind of composite microbic bacterial fertilizer and its preparation method and application | |
| CN116574771B (en) | Alginic acid fermentation broth and preparation method and application thereof | |
| CN109526974A (en) | A kind of composition and application method of efficient prevention and treatment rice blast | |
| WO2022088283A1 (en) | Preparation method for silkworm cordyceps militaris concentrated solution | |
| CN106831129A (en) | The preparation method and straw mushroom breeding method of straw mushroom compost |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 20959444 Country of ref document: EP Kind code of ref document: A1 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| 122 | Ep: pct application non-entry in european phase |
Ref document number: 20959444 Country of ref document: EP Kind code of ref document: A1 |