CN108689759A - Organic-biological stimulin and its preparation application method - Google Patents
Organic-biological stimulin and its preparation application method Download PDFInfo
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- CN108689759A CN108689759A CN201810609612.9A CN201810609612A CN108689759A CN 108689759 A CN108689759 A CN 108689759A CN 201810609612 A CN201810609612 A CN 201810609612A CN 108689759 A CN108689759 A CN 108689759A
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- organic
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- biostimulant
- biological stimulin
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of organic-biological stimulin, are made of the raw material of following parts by weight:The complex micro organism fungicide of the cane molasses of 46~66 parts by weight, the biostimulant of 15~25 parts by weight, 6~10 parts by weight, wherein, biostimulant is at least two combination of maltitol, xylitol, shrimp protein, chitosan oligosaccharide, and complex micro organism fungicide is at least two combination of bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete.The invention also discloses a kind of configuration application methods of organic-biological stimulin.The present invention can make crop yield 10%-20%, promote disease-resistant rate and fruit quality, reduce fertilizer amount, cost-effective.
Description
Technical field
The present invention relates to agriculture field, specifically a kind of organic-biological stimulin and its preparation application method.
Background technology
Cane molasses are the juice from sugar cane that sugar industry will squeeze out, heated, neutralization, precipitation, filtering, concentration, crystallization
Etc. dense fluids remaining after processes sugaring, be commonly called as malt sugar.Belong to liquid energy feed.It is former that molasses are not only a kind of energy
Material, but also have many advantages, such as to digest and assimilate fast, improvement palatability, reduce dust, improve granular mass.Biostimulant is that have
The quick-acting organic matters of bioactivity, have the function of promote growth, increase resistance, biostimulant fertilizer transition and upgrade,
Application prospect is huge in terms of upgrading synergy.Most of existing fertilizer is inorganic fertilizer and organic fertilizer, and dosage is big, to a certain degree
On promote the growths of crops, but it contains and is largely difficult to the substance degraded, and long-time service can cause soil to be hardened, lump,
Crops continue the underproduction, bring harm to environment, do not meet environmentally protective theory.Therefore, exploitation one kind can it is cost-effective,
The new-type fertilizer for reducing fertilizer amount, raising crops quality and yield is very necessary.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of organic-biological stimulin and its application method is prepared, agriculture can be made
Crop yield 10%-20% promotes disease-resistant rate and fruit quality, reduces fertilizer amount, cost-effective.
In order to realize these purposes and other advantages according to the present invention, provide a kind of organic-biological stimulin, by with
The raw material of lower parts by weight is made:The cane molasses of 46~66 parts by weight, the biostimulant of 15~25 parts by weight, 6~10 weight
Part complex micro organism fungicide, wherein biostimulant be maltitol, xylitol, shrimp protein, chitosan oligosaccharide at least two
Combination, complex micro organism fungicide are bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, yeast
At least two combination of bacterium.
The present invention also provides a kind of preparation application methods of organic-biological stimulin, include the following steps:
Step 1: by the cane molasses of 46~66 parts by weight, the complex micro organism fungicide of 6~10 parts by weight, 15~25 weights
The biostimulant for measuring part is uniformly mixed, and forms mixed liquor;
48~72h Step 2: the mixed liquor that step 1 is obtained ferments under conditions of 24~30 DEG C, obtains organic-biological
Stimulin;
Wherein, biostimulant is at least two combination of maltitol, xylitol, shrimp protein, chitosan oligosaccharide, compound micro-
Bacteria agent be bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete at least two
Combination.
Preferably, 200~300 times of organic-biological stimulin dilution made from step 2 pours and imposes on crops.
Preferably, organic-biological stimulin made from step 2 dilutes 800~1000 times and is sprayed in corps leaf surface
It applies.
Preferably, organic-biological stimulin made from step 2 applies crops progress trickle irrigation according to 2~3 kgs/acre
Fertilizer.
Preferably, the cane molasses in step 1 are pre-processed before mixing:By cane molasses first with 60 DEG C of temperature
Water is diluted, and the potassium ferrocyanide that mass fraction is 0.2% is then added, adjusts pH to 5.5, is heated to 105 DEG C of heat preservations
10min is cooled to 60 DEG C, then the sulphur acid for adjusting pH that addition mass fraction is 0.5% is added activated carbon and carries out ultrasound to 3.0
Oscillation treatment is irradiated using ultraviolet light during sonic oscillation, and polyacrylamide dry powder is added later, stirs evenly, and stands 2h, mistake
Filtrate is collected in filter, wherein the volume ratio of cane molasses and warm water is 5:6, the frequency of sonic oscillation is 25kHz, the time is
20min, ultraviolet light irradiation use wavelength for the UVB ultraviolet lights of 290nm.
Preferably, in step 1 biostimulant be shrimp protein and chitosan oligosaccharide combination, and shrimp protein and chitosan oligosaccharide
Weight ratio is 2:3, wherein shrimp protein is made by the following method:Shrimp head and shrimp shell are crushed, add water to stir evenly, hard
The ultra violet lamp 15min that rate is 55 watts, wavelength is 220nm, is then digested, and obtains enzymolysis liquid, then enzymolysis liquid is carried out
Decompression filters, and it is 30g/L to collect filtrate and the concentrated sulfuric acid to sulfuric acid concentration is added, and 100 DEG C of stirring hydrolysis 6h is heated to, after hydrolysis
It is 7 to be neutralized to pH with saturation sodium hydrate aqueous solution, and it is 25% to be concentrated in vacuo to solid content, and spray drying obtains powdered shrimp egg
In vain.
The present invention includes at least following advantageous effect:
The present invention adds beneficial organism and biostimulant using a large amount of cane molasses are generated in local sugar industry,
Through everfermentation, produce organic-biological stimulin product, for pour apply, foliage-spray or trickle irrigation, crop growth can be promoted, improved
The resistance of crops, reduces pest and disease damage, can improve crop yield and improve crop quality.Raw material used in the present invention is honest and clean
Valence is easy to get, cost-effective, makes crop yield 10%-20%, promotes disease-resistant rate and fruit quality.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail, to enable those skilled in the art with reference to specification
Word can be implemented according to this.
<Embodiment 1>
A kind of organic-biological stimulin, is made of the raw material of following parts by weight:The cane molasses of 46 parts by weight, 15 parts by weight
Biostimulant, 6 parts by weight complex micro organism fungicide, wherein biostimulant be maltitol, xylitol, shrimp protein,
At least two combination of chitosan oligosaccharide, complex micro organism fungicide is bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic
At least two combination of probiotics, lactic acid bacteria, saccharomycete.
The preparation application method of above-mentioned organic-biological stimulin is as follows:
Step 1: the biology of the cane molasses of 46 parts by weight, the complex micro organism fungicide of 6 parts by weight, 15 parts by weight is pierced
Hormone is uniformly mixed, and forms mixed liquor;
Step 2: the mixed liquor that step 1 obtains is fermented 48h under conditions of 24 DEG C, organic-biological stimulin is obtained;
Wherein, biostimulant is at least two combination of maltitol, xylitol, shrimp protein, chitosan oligosaccharide, compound micro-
Bacteria agent be bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete at least two
Combination.
<Embodiment 2>
A kind of organic-biological stimulin, is made of the raw material of following parts by weight:The cane molasses of 56 parts by weight, 20 parts by weight
Biostimulant, 8 parts by weight complex micro organism fungicide, wherein biostimulant be maltitol, xylitol, shrimp protein,
At least two combination of chitosan oligosaccharide, complex micro organism fungicide is bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic
At least two combination of probiotics, lactic acid bacteria, saccharomycete.
The preparation application method of above-mentioned organic-biological stimulin is as follows:
Step 1: the biology of the cane molasses of 56 parts by weight, the complex micro organism fungicide of 8 parts by weight, 20 parts by weight is pierced
Hormone is uniformly mixed, and forms mixed liquor;
Step 2: the mixed liquor that step 1 obtains is fermented 60h under conditions of 26 DEG C, organic-biological stimulin is obtained;
Wherein, biostimulant is at least two combination of maltitol, xylitol, shrimp protein, chitosan oligosaccharide, compound micro-
Bacteria agent be bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete at least two
Combination.
<Embodiment 3>
A kind of organic-biological stimulin, is made of the raw material of following parts by weight:The cane molasses of 66 parts by weight, 25 parts by weight
Biostimulant, 10 parts by weight complex micro organism fungicide, wherein biostimulant be maltitol, xylitol, shrimp egg
In vain, at least two combination of chitosan oligosaccharide, complex micro organism fungicide are bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, light
Close at least two combination of probiotics, lactic acid bacteria, saccharomycete.
The preparation application method of above-mentioned organic-biological stimulin is as follows:
Step 1: the biology of the cane molasses of 66 parts by weight, the complex micro organism fungicide of 10 parts by weight, 25 parts by weight is pierced
Hormone is uniformly mixed, and forms mixed liquor;
Step 2: the mixed liquor that step 1 obtains is fermented 72h under conditions of 30 DEG C, organic-biological stimulin is obtained;
Wherein, biostimulant is at least two combination of maltitol, xylitol, shrimp protein, chitosan oligosaccharide, compound micro-
Bacteria agent be bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete at least two
Combination.
<Embodiment 4>
On the basis of embodiment 2, the cane molasses in step 1 are pre-processed before mixing:By cane molasses elder generation
It is diluted with 60 DEG C of warm water, the potassium ferrocyanide that mass fraction is 0.2% is then added, adjusts pH to 5.5, is heated to 105
DEG C heat preservation 10min, is cooled to 60 DEG C, and sulphur acid for adjusting pH that mass fraction is 0.5% is added to 3.0, be then added activated carbon into
Row sonic oscillation is handled, and is irradiated using ultraviolet light during sonic oscillation, and polyacrylamide dry powder is added later, stirs evenly, quiet
2h is set, is filtered, filtrate is collected, wherein the volume ratio of cane molasses and warm water is 5:6, the frequency of sonic oscillation be 25kHz, when
Between be 20min, ultraviolet light irradiation uses wavelength for the UVB ultraviolet lights of 290nm.
<Embodiment 5>
On the basis of embodiment 2, in step 1 biostimulant be shrimp protein and chitosan oligosaccharide combination, and shrimp protein with
The weight ratio of chitosan oligosaccharide is 2:3, wherein shrimp protein is made by the following method:Shrimp head and shrimp shell are crushed, add water stirring equal
It is even, it with the ultra violet lamp 15min that power is 55 watts, wavelength is 220nm, is then digested, obtains enzymolysis liquid, then will enzymolysis
Liquid carries out decompression suction filtration, and it is 30g/L to collect filtrate and the concentrated sulfuric acid to sulfuric acid concentration is added, and is heated to 100 DEG C of stirring hydrolysis 6h, hydrolysis
After with saturation sodium hydrate aqueous solution be neutralized to pH be 7, be concentrated in vacuo to solid content be 25%, spray drying obtain powder
Shape shrimp protein.
<Embodiment 6>
On the basis of embodiment 2, the cane molasses in step 1 are pre-processed before mixing:By cane molasses elder generation
It is diluted with 60 DEG C of warm water, the potassium ferrocyanide that mass fraction is 0.2% is then added, adjusts pH to 5.5, is heated to 105
DEG C heat preservation 10min, is cooled to 60 DEG C, and sulphur acid for adjusting pH that mass fraction is 0.5% is added to 3.0, be then added activated carbon into
Row sonic oscillation is handled, and is irradiated using ultraviolet light during sonic oscillation, and polyacrylamide dry powder is added later, stirs evenly, quiet
2h is set, is filtered, filtrate is collected, wherein the volume ratio of cane molasses and warm water is 5:6, the frequency of sonic oscillation be 25kHz, when
Between be 20min, ultraviolet light irradiation uses wavelength for the UVB ultraviolet lights of 290nm.
Biostimulant is the combination of shrimp protein and chitosan oligosaccharide in step 1, and the weight ratio of shrimp protein and chitosan oligosaccharide is
2:3, wherein shrimp protein is made by the following method:Shrimp head and shrimp shell are crushed, water is added to stir evenly, is 55 watts, wave with power
The ultra violet lamp 15min of a length of 220nm, is then digested, and obtains enzymolysis liquid, then enzymolysis liquid is carried out decompression suction filtration, is received
Integrate filtrate and the concentrated sulfuric acid is added to sulfuric acid concentration as 30g/L, 100 DEG C of stirring hydrolysis 6h is heated to, with saturation hydrogen-oxygen after hydrolysis
It is 7 to change sodium water solution to be neutralized to pH, and it is 25% to be concentrated in vacuo to solid content, and spray drying obtains powdered shrimp protein.
The organic-biological stimulin prepared in embodiment 1-6 dilutes 200~300 times and pours respectively imposes on crops.
The organic-biological stimulin prepared in embodiment 1-6 dilutes 800~1000 times and is sprayed in corps leaf surface respectively
It applies.
The organic-biological stimulin prepared in embodiment 1-6 carries out trickle irrigation to crops according to 2~3 kgs/acre respectively and applies
Fertilizer.
<Dui Bishiyan >
Comparative example 1 be using pachyrhizus lateral bud, stem, leaf as primary raw material, addition brown sugar, fructose, agar, steamed beef soup, zinc sulfate,
One kind or arbitrary proportion in potassium carbonate, lime, potassium dihydrogen phosphate and ammonium sulfate it is two or more, be uniformly mixed, ferment,
Fermentation temperature be 15~40 DEG C, fermentation 18 days after, adjust pH to 5.5~8.5, after sterilizing press filtration obtain biostimulant.
Comparative example 2 is remaining ingredient, parameter and preparation process and embodiment 2 using pachyrhizus lateral bud, stem, leaf as primary raw material
It is identical, prepare biostimulant.
The biostimulant that organic-biological stimulin prepared by embodiment 1-6 is prepared with comparative example 1-2 respectively according to
Lower three kinds of modes (I/II/III) are administered to cassava and watermelon (cassava and watermelon plant 1 mu and apply fertilizer 3 times respectively), and divide
Do not counted growing height in certain time, whole disease-resistant rate, yield and harvest fresh cassava starch average content and
The average sugar content of the watermelon of harvest, wherein the growing height in certain time is the number that cassava measures in same time period
Value (cassava from growth 140 days to 170 days), as shown in table 1.
I, 260 times of dilution pours and imposes on cassava respectively, and interval is 15 days;
II, 900 times of dilution is sprayed on watermelon blade face respectively, and interval is 15 days;
III, respectively according to 2 kgs/acre to cassava carry out fertigation, interval be 15 days.
Table 1
As shown in Table 1, after organic-biological stimulin prepared by the method for the present invention is for cassava and watermelon, make cassava
10%-20% is improved with the yield of watermelon, and the average content of disease-resistant rate and starch is improved.In addition, according to practical life
Statistics in production can make utilization rate of fertilizer at least improve 5-9% using the biostimulant of invention, and pesticide dosage saves 5%-
10%.
In addition, taking average numerical value after organic-biological stimulin prepared by the present invention is detected, obtains it and contain organic matter
Content >=125g/L, NP2O5K2O >=100g/L, ZnMnB >=30g/L, living bacteria count (cfu) >=200,000,000/g.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (7)
1. organic-biological stimulin, which is characterized in that be made of the raw material of following parts by weight:The cane suger of 46~66 parts by weight
The complex micro organism fungicide of honey, the biostimulant of 15~25 parts by weight, 6~10 parts by weight, wherein biostimulant is malt
At least two combination of sugar alcohol, xylitol, shrimp protein, chitosan oligosaccharide, complex micro organism fungicide are bacillus subtilis, colloid bud
At least two combination of spore bacillus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete.
2. the preparation application method of organic-biological stimulin as described in claim 1, which is characterized in that include the following steps:
Step 1: by the cane molasses of 46~66 parts by weight, the complex micro organism fungicide of 6~10 parts by weight, 15~25 parts by weight
Biostimulant be uniformly mixed, formed mixed liquor;
48~72h Step 2: the mixed liquor that step 1 is obtained ferments under conditions of 24~30 DEG C obtains organic-biological stimulation
Element;
Wherein, biostimulant is at least two combination of maltitol, xylitol, shrimp protein, chitosan oligosaccharide, complex microorganism
Microbial inoculum be bacillus subtilis, colloid bacillus cereus, nitrogen-fixing bacteria, photosynthetic probiotics, lactic acid bacteria, saccharomycete at least two group
It closes.
3. the preparation application method of organic-biological stimulin as claimed in claim 2, which is characterized in that have made from step 2
200~300 times of machine biostimulant dilution, which is poured, imposes on crops.
4. the preparation application method of organic-biological stimulin as claimed in claim 2, which is characterized in that have made from step 2
Machine biostimulant dilutes 800~1000 times and is sprayed in corps leaf surface.
5. the preparation application method of organic-biological stimulin as claimed in claim 2, which is characterized in that have made from step 2
Machine biostimulant carries out fertigation according to 2~3 kgs/acre to crops.
6. the preparation application method of organic-biological stimulin as claimed in claim 2, which is characterized in that the sugarcane in step 1
Molasses are pre-processed before mixing:Cane molasses are first diluted with 60 DEG C of warm water, mass fraction, which is then added, is
0.2% potassium ferrocyanide adjusts pH to 5.5, is heated to 105 DEG C of heat preservation 10min, is cooled to 60 DEG C, and mass fraction, which is added, is
Then 0.5% sulphur acid for adjusting pH is added activated carbon and carries out sonic oscillation processing, ultraviolet light is used during sonic oscillation to 3.0
Irradiation is added polyacrylamide dry powder, stirs evenly later, stands 2h, and filtering collects filtrate, wherein cane molasses and warm water
Volume ratio be 5:6, the frequency of sonic oscillation is 25kHz, time 20min, and ultraviolet light irradiation uses wavelength for 290nm's
UVB ultraviolet lights.
7. the preparation application method of organic-biological stimulin as claimed in claim 2, which is characterized in that biology thorn in step 1
Hormone is the combination of shrimp protein and chitosan oligosaccharide, and the weight ratio of shrimp protein and chitosan oligosaccharide is 2:3, wherein shrimp protein by with
Lower section method is made:Shrimp head and shrimp shell are crushed, add water to stir evenly, with the ultra violet lamp that power is 55 watts, wavelength is 220nm
Then 15min is digested, obtain enzymolysis liquid, then enzymolysis liquid is carried out decompression suction filtration, collects filtrate and the concentrated sulfuric acid is added to sulfuric acid
A concentration of 30g/L is heated to 100 DEG C of stirring hydrolysis 6h, and it is 7 to be neutralized to pH with saturation sodium hydrate aqueous solution after hydrolysis,
It is 25% to be concentrated in vacuo to solid content, and spray drying obtains powdered shrimp protein.
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| CN113185350A (en) * | 2021-04-13 | 2021-07-30 | 中科韶华(四川)牧业研究院有限公司 | Weeding type bio-organic fertilizer, preparation method and application |
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Application publication date: 20181023 |
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