CN104744160A - Biocompound fertilizer for preventing and controlling sclerotinia and application method thereof - Google Patents

Biocompound fertilizer for preventing and controlling sclerotinia and application method thereof Download PDF

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Publication number
CN104744160A
CN104744160A CN201510108268.1A CN201510108268A CN104744160A CN 104744160 A CN104744160 A CN 104744160A CN 201510108268 A CN201510108268 A CN 201510108268A CN 104744160 A CN104744160 A CN 104744160A
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China
Prior art keywords
fertilizer
bacterium slag
composite fertilizer
fermented product
biocompound
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CN201510108268.1A
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CN104744160B (en
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刘勇
黄小琴
张蕾
伍文宪
周西全
刘红雨
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Institute of Plant Protection Sichuan Academy of Agricultural Sciences
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Institute of Plant Protection Sichuan Academy of Agricultural Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention discloses a biocompound fertilizer for preventing and controlling sclerotinia. The biocompound fertilizer is characterized by comprising the following components in parts by weight: 80-90 parts of fungi residue yeast and 10-20 parts of inorganic compound fertilizer, wherein the fungi residue yeast is obtained by fermenting the fungi residue with coniothyrium; in the fungi residue yeast, the coniothyrium spore concentration is 10<8> spore/g fungi residue; in the inorganic compound fertilizer, the content of nitrogen, phosphorus and potassium is greater than or equal to 90wt%. The raw material of the biocompound fertilizer is edible fungi residue; the fungi residue is fermented to produce coniothyrium and therefore the fungi residue can be changed into things of value; inorganic compound fertilizer is added into the yeast properly to form the biocompound fertilizer. The biocompound fertilizer can effectively increase the organics in soil and obviously prevent and control sclerotinia; the application mode is not strict and any fertilizing mode in the agricultural meaning can be used.

Description

A kind of composite bio-fertilizer and application method thereof preventing and treating sclerotium disease
Technical field
The invention belongs to composite bio-fertilizer field, be specifically related to a kind of composite bio-fertilizer and the application method thereof of preventing and treating sclerotium disease.
Technical background
Sclerotium disease is by Sclerotinia (Sclerotinia) fungus-caused Plant diseases, the stem of main harm plant is climing, blade and fruit, site of pathological change is combined into structure degree of tightness by mycelium and differs, smooth surface or coarse, the sclerotium that shape, size, color are different.Be injured plant except the plants such as common rape, vegetables, also have the crops such as Chinese medicinal materials, flowers, mulberry, leguminous forage.
Mainly also resting on for the controlling mode of sclerotium disease at present uses on the chemical prevention and control method such as derosal or dimetachlone, produces serious disadvantageous effect to the pollution of soil and sustainable use.
In the early days of foundation, the average organic content of soil is 3.7%, and even to this day, the average organic content of soil only has 1.7% in China.Soil organic matter content is most important for the growth of farm crop, and the factor impact nutritive substance in soil, soil physico-chemical property etc. being affected to crop growth is huge.
Therefore, for the cultivation of the crop of susceptible sclerotium disease, use and can prevent and treat the fertilizer that sclerotium disease can improve again soil organic matter content, seem very necessary.
Summary of the invention
For the shortcoming of prior art, an object of the present invention is to provide a kind of composite bio-fertilizer preventing and treating sclerotium disease, and with parts by weight, this composite fertilizer comprises following component:
Bacterium slag fermented product 80-90 part
Inorganic composite fertilizer 10-20 part
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell, and in described bacterium slag fermented product, Coniothyrium minitans concentration is 10 8individual spore/g bacterium slag;
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium >=90wt%.
Bacterium slag has higher organic content; usually can be used as cultivation matrix to use; but the edible mushrooms containing a large amount of live body in bacterium slag; directly be applied in soil; not only easily cause soil Flora Disturbance; and unpasteurized bacterium slag also continued growth can go out edible mushrooms individuality when being directly applied in soil, reduce the output of farm crop on the contrary, the edible fungi residue without sterilizing even can bring the pathogenic bacterium of plant into.Simple bacterium slag after thermophilic fermentation, although directly can be applied to soil, to improve the soil organism, its too high cost peasant household can not be accepted and accepted with agriculture production, more without the effect of preventing and treating sclerotium disease.The present inventor is through long-felt, and find to utilize that shield shell is mould can carry out innoxious fermentative processing to bacterium slag, after fermentation, the mould dominant bacteria become in bacterium slag of shield shell, can efficiently suppress even to remove the miscellaneous bacteria in bacterium slag.Because shield shell is mould, there is the effect of preventing and treating sclerotium disease efficiently, while making gained bacterium slag fermented product of the present invention may be used for improving the soil organism, the effect of preventing and treating sclerotium disease significantly can also be played.
Existing result of study display, the fermented substrate of current shield shell mould based on wheat or crop material, and comes into operation after usually needing wash-out spore to be prepared into microbial inoculum after fermentation again.Because the process in early stage that wheat fermentative production shield shell removing mildew cost is high, use crop material to produce is loaded down with trivial details, the complete interlude formed to microbial inoculum and come into operation that ferments is complicated, and equipment requirements height waits factors to constrain the Application and Development of the mould biological prevention and control agent of shield shell to a certain extent.
Therefore bacterium slag is utilized to cultivate shield shell mould as substrate fermentation, not only eliminate the cumbersome process of crushing straw, and itself be rich in edible fungus mycelium and residue nutritive substance due to bacterium slag, therefore need not add a large amount of auxiliary materials or other nutritive substances again when the shield shell of fermenting is mould; Compared with wheat, bacterium slag source is wide, and cost is low.
In addition, bacterium slag is through the mould Secondary Fermentation of shield shell, the hard-degraded substance such as Mierocrystalline cellulose, xylogen reduces greatly, can directly fermented product be sprinkled in soil, achieve and can improve soil organic matter content while preventing and treating sclerotium disease, not only there is effect of fertile medicine integration, also make the harmless treatment by product of bacterium slag achieve zero residue.
Inorganic fertilizer add the fertility can optimizing composite bio-fertilizer of the present invention, be beneficial to the growth of farm crop.
Preferably, described bacterium slag fermented product is 85 parts, and inorganic complex is 15 parts.
Preferably, described bacterium slag fermented product is for add in bacterium slag by Coniothyrium minitans, and control temperature is at 18-21 DEG C, and ferment 15-18 days and obtain, described Coniothyrium minitans concentration is 10 6individual spore/g bacterium slag.
In the present invention, bacterium slag fermented product utilizes the fermentation of shield shell mould participation bacterium slag and obtains, and it is worthy of note no matter use which kind of fermentation mode, as long as the concentration of shield shell mould is 10 in bacterium slag fermented product 8individual spore/g bacterium slag, just can realize effect of the present invention.When fermentation mode is set to: add in bacterium slag by mould for shield shell, control temperature at 18-21 DEG C, bacterium slag fermentation needed for shortest time.
Second object of the present invention is the application method providing above-mentioned composite bio-fertilizer, and the method is: described composite fertilizer imposed in soil with 40-50Kg/ mu.
The method of application of described composite fertilizer is any one fertilization mode in agriculture meaning.
Preferably, the method for application of described composite fertilizer is for spreading fertilizer over the fields.
Beneficial effect of the present invention:
1, the raw material of composite bio-fertilizer is bacterium slag, wide material sources, bacterium slag can be turned waste into wealth;
2, while composite bio-fertilizer of the present invention effectively can improve the soil organism, the generation of sclerotium disease can also significantly be prevented and treated;
3, the method for application of composite bio-fertilizer of the present invention requires not harsh, the fertilization mode in any one agriculture meaning available.
Embodiment
Embodiment 1
Bacterium slag fermented product 80 parts
Inorganic composite fertilizer 20 parts
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell.Fermentation mode is: add in bacterium slag by shield shell mould liquid, and take shelter from rain and stack fermentation 30 days in outdoor, the concentration of described shield shell mould is 10 6individual spore/g bacterium slag.
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium are 90wt%.
By described composite bio-fertilizer with 40 kg/m 2impose in soil, fertilization mode is for spreading fertilizer over the fields.
Embodiment 2
Bacterium slag fermented product 90 parts
Inorganic composite fertilizer 10 parts
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell.Fermentation mode is: add in bacterium slag by shield shell mould liquid, and take shelter from rain and stack fermentation 25 days in outdoor, the concentration of described shield shell mould is 10 6individual spore/g bacterium slag.
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium are 92wt%.
By described composite bio-fertilizer with 50 kg/m 2impose in soil, fertilization mode is for spreading fertilizer over the fields.
Embodiment 3
Bacterium slag fermented product 85 parts
Inorganic composite fertilizer 15 parts
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell.Fermentation mode is: add in bacterium slag by shield shell mould liquid, and control temperature, at 21 DEG C, ferments 15 days, and the concentration of described shield shell mould is 10 6individual spore/g bacterium slag.
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium are 90wt%.
By described composite bio-fertilizer with 40 kg/m 2impose in soil, fertilization mode is row replacement.
Embodiment 4
Bacterium slag fermented product 85 parts
Inorganic composite fertilizer 15 parts
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell.Fermentation mode is: add in bacterium slag by shield shell mould liquid, and control temperature, at 18 DEG C, ferments 18 days, and the concentration of described shield shell mould is 10 6individual spore/g bacterium slag.
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium are 95wt%.
By described composite bio-fertilizer with 45 kg/m 2impose in soil, fertilization mode is row replacement.
Embodiment 5
Bacterium slag fermented product 85 parts
Inorganic composite fertilizer 15 parts
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell.Fermentation mode is: add in bacterium slag by shield shell mould liquid, and control temperature, at 20 DEG C, ferments 16 days, and the concentration of described shield shell mould is 10 6individual spore/g bacterium slag.
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium are 95wt%.
By described composite bio-fertilizer with 50 kg/m 2impose in soil, fertilization mode is for spreading fertilizer over the fields.
Test example 1
Embodiment 1-5 is to preventing and treating sclerotinia rot of colza effect in table 1.Wherein control group is not for use composite bio-fertilizer of the present invention.Unit square soil sclerotinite sclerotium quantity and rape seedling sclerotium disease incidence all with control group for 100%.A represents unit square soil sclerotinite sclerotium quantity, and B represents rape seedling sclerotium disease incidence.
Table 1
Test example 2
Embodiment 1-5 to the raising effect of soil organic matter content in table 2.Before not using composite bio-fertilizer of the present invention, through measuring, the organic content of soil is 3.1%.
Table 2

Claims (6)

1. prevent and treat a composite bio-fertilizer for sclerotium disease, it is characterized in that, with parts by weight, described composite fertilizer comprises following component:
Bacterium slag fermented product 80-90 part
Inorganic composite fertilizer 10-20 part
Described bacterium slag fermented product obtains for utilizing mould the carrying out after fermentation bacterium slag of shield shell, and in described bacterium slag fermented product, Coniothyrium minitans concentration is 10 8individual spore/g bacterium slag;
In described inorganic composite fertilizer, nitrogen, phosphorus, potassium >=90wt%.
2. composite fertilizer according to claim 1, is characterized in that, described bacterium slag fermented product is 85 parts, and inorganic complex is 15 parts.
3. composite fertilizer according to claim 1, is characterized in that, described bacterium slag fermented product is for add in bacterium slag by Coniothyrium minitans, and control temperature is at 18-21 DEG C, and ferment 15-18 days and obtain, described Coniothyrium minitans concentration is 10 8individual spore/g bacterium slag.
4. the application method of composite fertilizer according to claim 1, is characterized in that, described composite fertilizer is imposed in soil with 40-50Kg/ mu.
5. application method according to claim 4, is characterized in that, the method for application of described composite fertilizer is any one fertilization mode in agriculture meaning.
6. the application method according to claim 4 or 5, is characterized in that, the method for application of described composite fertilizer is for spreading fertilizer over the fields.
CN201510108268.1A 2015-03-12 2015-03-12 A kind of biological compound fertilizer and its application process for preventing and treating sclerotiniose Active CN104744160B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105016795A (en) * 2015-07-24 2015-11-04 芜湖欧标农业发展有限公司 Organic fertilizer prepared from solidago Canadensis and cinnamomum camphora leaf
CN108976031A (en) * 2018-08-31 2018-12-11 怀化远东化肥有限公司 A kind of chelate compound fertilizer and preparation method thereof of the long prevention and treatment sclerotiniose of fertilizer efficiency
CN110396028A (en) * 2019-07-10 2019-11-01 湖北新保得生物科技有限公司 A kind of bio-organic fertilizer and application suitable for rape
CN112409091A (en) * 2019-08-21 2021-02-26 华中农业大学 Bacterial fertilizer composition based on mixed application of coniothyrium minitans and rape long-acting special formula fertilizer and application thereof
CN113416104A (en) * 2021-06-10 2021-09-21 中国农业科学院油料作物研究所 Spraying type preparation for biologically preventing sclerotinia and promoting high yield of rape and preparation method thereof

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CN102234619A (en) * 2011-06-16 2011-11-09 四川省农业科学院植物保护研究所 Biocontrol bacteria coniothyrium minitans Cm2004 strain for preventing and treating sclerotiniose and preparation method and application thereof
CN102408996A (en) * 2010-09-26 2012-04-11 华中农业大学 Wettable powder of biocontrol bacteria coniothyrium minitans Chy-1C-1 and application
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105016795A (en) * 2015-07-24 2015-11-04 芜湖欧标农业发展有限公司 Organic fertilizer prepared from solidago Canadensis and cinnamomum camphora leaf
CN108976031A (en) * 2018-08-31 2018-12-11 怀化远东化肥有限公司 A kind of chelate compound fertilizer and preparation method thereof of the long prevention and treatment sclerotiniose of fertilizer efficiency
CN110396028A (en) * 2019-07-10 2019-11-01 湖北新保得生物科技有限公司 A kind of bio-organic fertilizer and application suitable for rape
CN110396028B (en) * 2019-07-10 2021-08-03 湖北新保得生物科技有限公司 Biological organic fertilizer suitable for rape and application thereof
CN112409091A (en) * 2019-08-21 2021-02-26 华中农业大学 Bacterial fertilizer composition based on mixed application of coniothyrium minitans and rape long-acting special formula fertilizer and application thereof
CN113416104A (en) * 2021-06-10 2021-09-21 中国农业科学院油料作物研究所 Spraying type preparation for biologically preventing sclerotinia and promoting high yield of rape and preparation method thereof

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