CN110396028A - A kind of bio-organic fertilizer and application suitable for rape - Google Patents
A kind of bio-organic fertilizer and application suitable for rape Download PDFInfo
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- CN110396028A CN110396028A CN201910620698.XA CN201910620698A CN110396028A CN 110396028 A CN110396028 A CN 110396028A CN 201910620698 A CN201910620698 A CN 201910620698A CN 110396028 A CN110396028 A CN 110396028A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention belongs to bio-organic fertilizer fields, disclose a kind of bio-organic fertilizer suitable for rape and application, by weight percentage, including following raw material: distillers ' grains powder 45-55%, rape dregs of rice 20-25%, rape straw 15-25%, complex micro organism fungicide 0.3-0.5%, cray shell powder 5-8%.Biological organic fertilizer of the invention is to ferment to obtain by specific complex micro organism fungicide, and the present invention can efficiently use the agriculture and industry wastes such as distillers ' grains, rape stalk, cray shell, can reduce production cost, reduce sclerotinia sclerotiorum disease incidence, improves yield of rape.
Description
Technical field
The invention belongs to bio-organic fertilizer field, specifically discloses a kind of bio-organic fertilizer suitable for rape and answer
With.
Background technique
As the valuable cargo input in modern agricultural production, fertilizer is ensureing crop high and stable yield and quality potentiality hair
Particularly important irreplaceable role is played in waving.China's rapeseed cultivation area crosses hundred million mu, other rapes are main with the world
Producing country compares, and China's rapeseed cultivation entirety land occupation condition is poor, soil organic matter content is low, nutrient dificiency type mostly and
Degree is high, soil fertilizer ability is poor, causes soil basic topography level lower, in addition China's major part rape is made as winter
The factors such as species are planted, and temperature is low, soil moisture is excessive insufficient with soil moisture content and deposits cause soil nutrient deliverability to decline.More than
Various factors is superimposed, and current China's rape main producing region average basal soil fertility is caused only to be able to satisfy rape high yield to nutrient
50% or so of demand.Currently, the fertilizer of rape is usually using traditional compound fertilizer or organic fertilizer etc., and it is traditional compound
Fertilizer formula is more single, cannot sufficiently meet the needs of rapeseed plants growth, and implementation result is unobvious.And organic fertilizer is usual
It is worth based on feces of livestock and poultry, containing the harmful substances such as a large amount of pathogenic bacteria and worm's ovum, heavy metal if fermentation is not thorough, directly
Application can contaminated soil.Fowl and animal excrement is handled by biofermentation technique mostly now, make hydrogen sulfide in fowl and animal excrement,
The malodorous elements such as indoles, amine are cleared up rapidly, moreover it is possible to make the Degradation and Transformations such as cellulose, the protein in fowl and animal excrement to plant
Substance that is beneficial and can directly absorbing.And existing organic fertilizer biofermentation technique has the disadvantage in that and mostly uses feces of livestock and poultry
Material mix variance is big, and fermentation period is long, kills harmful bacteria and worm's ovum is not thorough, organic substance conversion ratio is low, deodorizing effect
It is poor, may there are heavy metal or medicament residue that there is some potential safety problems.
For this reason, it is necessary to a kind of bio-organic fertilizer that rapeseed plants can be promoted to grow be studied, by vegetable raw material
Harmless treatment, be constructed to promote rapeseed plants growth, it is that soil can be improved, can improve rapeseed quality, yield can be improved
Efficient biologic-organic fertilizer.The rape dregs of rice and rape stalk derive from cole crop, and the major part for meeting cole crop growth is supported
Divide demand, but directly application then has the potential security risk of plant pathogenic microorganisms infection crop.The present invention with the rape dregs of rice and
It is micro- to carry out decomposed and pathogenic by complex microorganism During High-Temperature Composting for primary raw material of the rape stalk as organic fertilizer fermentation
The killing of biology, production are suitable for the biological organic fertilizer of growth of rape demand.
Summary of the invention
The object of the present invention is to provide a kind of biological organic fertilizer suitable for rape, which utilizes rape stalk, white
The wastes such as vinasse are free of harmful bacteria and worm's ovum, and the content of the poisonous and harmful substances such as heavy metal free can reduce production cost,
Improve rapeseed quality, improves yield.
It is another object of the present invention to provide a kind of applications of biological organic fertilizer suitable for rape.
In order to achieve the above object, the present invention takes following technical measures:
A kind of biological organic fertilizer suitable for rape is sent out by carrying out compost fermentation to material with complex microorganism preparations
It banks up and is aged after ferment to obtain the final product；
Component in the material is in parts by weight, comprising: distillers ' grains powder 45-55, rape dregs of rice 20-25, rape stalk
Powder 15-25, complex micro organism fungicide 0.3-0.5, cray shell powder 5-8；
The living bacteria count for meeting microbial bacterial agent is hundred million CFU/g of 110-150, comprising: thermophilic fungus destroyed wire bacterium 9-
1200000000 CFU/g, bacillus subtilis 80-120 hundred million CFU/g, trichoderma harzianum 1-3 hundred million CFU/g, bacillus coagulans 15-30
Hundred million CFU/g of hundred million CFU/g, Paenibacillus polymyxa 1-3, hundred million CFU/g of aspergillus oryzae 1-3, hundred million CFU/g of lactobacillus plantarum 3-5；
The thermophilic fungus destroyed wire are as follows: Myceliophthora thermophila MT1810, deposit number are as follows: CCTCC
NO:M2018705；
The aspergillus oryzae are as follows: aspergillus oryzae (Aspergillus oryzae) A08, deposit number are as follows: CCTCC NO:M
2019505；
The lactobacillus plantarum are as follows: lactobacillus plantarum (Lactobacillus plantaLum) L16, deposit number are as follows:
CCTCC NO:M2019502；
Remaining bacterial strain is commercialization bacterial strain；
Above-described biological organic fertilizer, it is preferred that the component in the material is in parts by weight, comprising: distillers ' grains
Powder 50, the rape dregs of rice 20, rape straw 24, complex micro organism fungicide 0.5, cray shell powder 5.5；
Above-described biological organic fertilizer, it is preferred that the component in the material is in parts by weight, comprising: distillers ' grains
Powder 55, the rape dregs of rice 15, rape straw 23, complex micro organism fungicide 0.3, cray shell powder 6.7；
Above-described biological organic fertilizer, it is preferred that daily turning after fermentation temperature is increased to 60 DEG C or more, daily 1-2
It is secondary；
Above-described biological organic fertilizer, it is preferred that the overall weight of material is the 50%-60% of fermentation initial stage weight in wet base,
That is fermentation ends；
Above-described biological organic fertilizer, it is preferred that bank up ageing 10-20 days.
A kind of application of the biological organic fertilizer suitable for rape, the organic fertilizer including being used to prepare into anti-sclerotinia sclerotiorum,
Or directly it is prepared into Rape organic fertilizer.
Compared with prior art, the invention has the characteristics that:
1. the primary raw material of fertilizer is the agriculture and industry wastes such as distillers ' grains, rape stalk, cray shell, and current
In organic bio-fertilizer, the raw materials such as feces of livestock and poultry are generallyd use.Feces of livestock and poultry usually contains pathogenic microorganisms, roundworm egg, heavy metal
With medicament residue etc., there is certain security risk.Biological organic fertilizer of the invention mainly uses plant substrates raw material, safety
Controllably, without above-mentioned risk.
2. being degraded by microorganisms in composting fermentation process and gathering for shell due to joined cray shell in preparation method
Sugar.Chitosan promotes absorption of the plant to N, C, P and mineral matter element, can also induce plant for adjusting soil nutrient
Disease resistance is generated, plant growth is promoted, improves crop quality.
3. preparation method using fermentation maturity technology is banked up, is decomposed using the metabolic activity of complex microorganism and conversion object
Organic matter in material makes nutrient and organic matter in material reach stable and innoxious；The bacterium independently screened is added simultaneously
Strain, increases the amino acid content in fertilizer, while having the effect of inhibiting rape endophytic bacterial.
4. biological organic fertilizer can improve soil, culture fertility provides nutrition for crops, may additionally facilitate edaphon
Activity, particularly suitable for rapeseed plants growth use, thus increase rape yield and improve rape quality.
Specific embodiment
According to following embodiment, the present invention can be better understood, but the embodiment described is to preferably explain this
Invention, rather than limiting the invention.Technical solution of the present invention is if not otherwise specified the routine side of this field
Case；The reagent or material derive from commercial channel if not otherwise specified.
The present embodiments relate to the bacterium source arrived is as follows:
The thermophilic fungus destroyed wire are as follows: Myceliophthora thermophila MT1810, deposit number are as follows: CCTCC
NO:M2018705；
The aspergillus oryzae are as follows: aspergillus oryzae (Aspergillus oryzae) A08, deposit number are as follows: CCTCC NO:M
2019505；
The lactobacillus plantarum are as follows: lactobacillus plantarum (lactobacillus plantaLum) L16, deposit number are as follows:
CCTCC NO:M2019502；
The bacillus subtilis are as follows: Bacillus subtilis, the limited public affairs of development in science and technology are raised by China from Hubei
Department；
The Trichoderma harzianum are as follows: Trichoderma harzianum, to the limited public affairs of bioengineering in Hubei
Department；
The bacillus coagulans are as follows: Bacillus coagulans, the limited public affairs of development in science and technology are raised by China from Hubei
Department；
The Paenibacillus polymyxa are as follows: Paenibacillus polymyxa derives from Wuhan section promise biotechnology
Co., Ltd；
The distillers ' grains powder used is to produce the distillers ' grains powder after white wine using Luzhou-flavor liquo technique by raw material of sorghum.
Embodiment 1:
The mutagenesis screening of aspergillus oryzae protease superior strain:
Co 60 mutagenesis is carried out in academy of agricultural sciences, Hubei Province Radiation Center.Fresh, spore aspergillus oryzae strain inclined-plane abundant is taken,
With suitable aseptic water washing spore for containing 0.1% Tween 80, blood cell plate, dilution apply plate count, guarantee spore count 107
A/mL or more.Respectively take 5ml spore suspension to be placed in 4 sterile triangular flasks, respectively acceptable dose be 185Gy, 395Gy,
The Co 60 gamma-ray irradiation of 605Gy, 815Gy, control do not receive irradiation.By each spore liquid received after radiation exposure, coating
In on casein medium, cultivating, counting and selecting big 70-80 plants of bacterium colony of transparent circle, save to the inclined-plane PDA.
The bacterial strain that primary dcreening operation is saved with bran mass culture, measures proteinase activity respectively, saves live high-enzyme strain；Together
When the live high-enzyme strain filtered out will be inoculated in using yellow wine lees as in the culture medium of main matrix, carry out enzyme activity determination, choose
Enzyme activity highest bacterial strain carries out next round mutagenesis.
By three-wheel Co 60 mutagenesis, screen that one plant of speed of growth is fast, the strong aspergillus oryzae superior strain of enzymatic productivity, in bran
With the inoculation of 1% (m/m) inoculum concentration on skin solid medium, grow 48h at 28 DEG C, proteinase activity up to 5150U/g (with
Butt meter).
The bacterial strain was preserved in China typical culture collection center (CCTCC) on July 1st, 2019, classification naming:
Aspergillus oryzae A08, deposit number are CCTCC NO:M 2019505, address: Wuhan, China Wuhan University.
Casein medium: casein 4g, MgSO4·7H2O 0.5g, Na2HPO4·7H2O 1.07g, KH2PO4
0.36g, agar 20g, 1000mL, pH6.5-7.0,121 DEG C of sterilizing 30min of distilled water.
PDA culture medium: potato (peeling) 200g, sucrose 20g, water 1000mL.By peeling potatoes, it is cut into small pieces, in
In pot plus water 1000mL boils half an hour, is filtered with double gauze, takes its filtrate sugaring, and water is added to supply 1000mL, agar
20g, pH are natural.121 DEG C of sterilizing 30min.
Bran mass: wheat bran 1000g, water 1000mL, pH are natural.121 DEG C of sterilizing 30min.
Embodiment 2:
The screening of lactobacillus plantarum L16 bacterial strain:
1. the preparation of culture medium:
BCP culture medium: peptone 5g；Yeast extract 3g；Lactose 5g；Agar 20g；0.5% bromocresol purple 10ml；Distilled water
1000ml；PH value 6.8~7.0.
MRS fluid nutrient medium: glucose 20g/L；Peptone 10g/L；Yeast extract 5g/L；Beef extract powder 10g/L；
Dibasic ammonium citrate 2g/L；Sodium acetate 5g/L；Tween 80 1g/L；K2HPO4 2g/L；MgSO4·7H2O 0.058g/L；
MnSO4·4H2O 0.25g/L；Distilled water 1000ml；pH6.5-6.8.
PDA culture medium: potato 200g；Glucose 20g；15~20g of agar；Distilled water 1000ml；Natural PH.
The agar of addition 1.5% on the basis of solid medium liquid medium within, 115 DEG C of all culture mediums sterilizings
20min。
2. primary dcreening operation: pickles, orchard Rhizosphere Soil, the rotten leaf in vegetable plot and each 5g of chopped hot pepper sample being taken to be placed in 300ml MLS liquid
After body culture medium culture 12h, 1ml culture solution gradient dilution is taken, BCP plate is coated on, selects wherein allusion quotation after 37 DEG C of culture 48h
The bacterium colony of type lactic acid bacteria colonial morphology (flavescence of bacterium colony periphery) carries out scribing line and is separately cultured, each Sample selection 5 or so, will
Obtained single colonie is transferred to MLS fluid nutrient medium culture 12h, MLS slant preservation.
3. secondary screening: carrying out plate secondary screening to 20 bacterial strains (number L1-L20) that primary dcreening operation obtains, be directed to tomato early epidemic respectively
Sick (pathogen: sporulation), cucumber anthracnose (pathogen: colletotrichum) and cucumber fusarium axysporum (sharp knife Fusariumsp
Cucumber specialized form) three kinds of frequently seen plants Pathogen detection its bacteriostasis properties, specific as follows:
It is beaten on PDA plate with the punch of diameter 0.6cm and takes cultured pathogen bacterium piece, transferred in another PDA
The lactic acid bacteria (number L1-L20) of activation, is respectively symmetrically seeded in away from pathogen colony edge 3cm by plate center with needle is chosen
Place, control only connect pathogen, later 26 DEG C of constant temperature incubations.When covering with plate wait compare, the average colony diameter of pathogen is measured
With the average antibacterial bandwidth of lactic acid bacteria, bacteriostasis rate is calculated, is weighed with the average bacteriostasis rate size of lactic acid bacteria with average antibacterial bandwidth
Measure the power of antagonism.
Finishing screen selects the antagonistic effect that bacterial strain L16 behaves oneself best to three kinds of pathogens, presses down to the plate of colletotrichum
Rate processed is 44.29%, is 40% to sporulation inhibiting rate, is 45.71% to sharp knife Fusariumsp cucumber transformant inhibiting rate
(table 1).Bacterial strain L16 shows preferable Biocontrol Potential, next carries out follow-up study by object of bacterial strain L16.
Inhibitory effect of the 1 bacterial strain L16 of table to phytopathogen
Bacterial strain L16 biochemical identification: the purpose bacterial strain L16 screened is simply dyed with crystal violet, and passes through light
Microscope is learned to be observed.It was found that the bacterial strain is gram-positive cocci, no brood cell is single, in pairs in straight or curved rod-shaped
Or at chain.Bacterium colony is that the smooth wet, droplet-like in milky, surface, circle, protuberance, edge are whole on MRS solid medium
Together, colony diameter about 3mm.Physiology and biochemistry identifies bacterial strain L16 amphimicrobian, does not restore nitrate, do not liquefy gelatin, catalase
It is all negative with oxidizing ferment.It can be grown in grape hydrochlorate, and produce CO2；Mono- lactic acid of L is produced using glucose homofermentative lactic；Hair
The ribose of 1 molecule of ferment or other pentoses generate the lactic acid of 1 molecule and the acetic acid of 1 molecule.Optimum growth temperature is 35 DEG C.
The 16s of bacterial strain is identified: picking L16 single colonie is expanded with universal primer, carries out 16S LLNA to amplified production
Sequencing analysis simultaneously constructs systematic evolution tree.The result shows that the sequence and Lactobacillus plantaLum
The 16S rDNA sequence of subsp.aLgentoLatens and Lactobacillus plantaLum subsp.plantaLum
Similitude highest, up to 99%, it may be determined that the bacterial strain is lactobacillus plantarum, is named as lactobacillus plantarum L16.The bacterial strain in
On June 28th, 2019 is sent to China typical culture collection center preservation, classification naming: Lactobacillus plantaLum
L16, deposit number are as follows: CCTCC NO:M2019502, address: Wuhan, China Wuhan University.
Embodiment 3:
Inhibitory effect of the lactobacillus plantarum L16 to rape pathogenic microorganism:
It is beaten on PDA plate with the punch of diameter 0.6cm and takes cultured rape pathogen bacterium piece, transferred in another
The lactobacillus plantarum L16 of activation is symmetrically seeded in away from pathogen colony edge 3cm with needle is chosen, is compareed by PDA plate center
Pathogen is only connect, later 26 DEG C of constant temperature incubations.When covering with plate wait compare, the average colony diameter and lactic acid of pathogen are measured
The average antibacterial bandwidth of bacterium, calculates bacteriostasis rate, measures antagonism with average antibacterial bandwidth with the average bacteriostasis rate size of lactic acid bacteria
The power of effect.
Bacterial strain L16 shows good antagonistic effect to two kinds of pathogens of rape, and the plate inhibiting rate to rape endophytic bacterial is
37.14%, it is 48.57% (table 2) to rape alternaric bacteria inhibiting rate, bacterial strain L16 shows preferable biological and ecological methods to prevent plant disease, pests, and erosion in rape and dives
Power.
Bacteriostatic activity of the 2 lactobacillus plantarum L16 of table to rape pathogen
Embodiment 4:
A kind of bio-organic fertilizer suitable for rape, is obtained by following step:
Distillers ' grains, rape stalk, cray shell are crushed to 2-3mm size；
500kg distillers ' grains powder, 200kg rape dregs of rice powder, 240kg rape straw, 55kg cray shell powder are mixed, adjustment
Moisture is to 55%, and inoculation 5kg complex micro organism fungicide is uniformly mixed, daily turning after fermentation temperature is increased to 60 DEG C or more, often
It 1-2 times, fermentation period 21 days, the overall weight of material was the 55% of fermentation initial stage weight in wet base.The environment temperature of the present embodiment is
20-35℃。
The total bacteria count of the complex micro organism fungicide is 14,000,000,000 CFU/g, comprising: 1,000,000,000 CFU/g of thermophilic fungus destroyed wire bacterium,
10,000,000,000 CFU/g of bacillus subtilis, 200,000,000 CFU/g of trichoderma harzianum, 2,000,000,000 CFU/g of bacillus coagulans, mostly viscous class gemma bar
200,000,000 CFU/g of bacterium, aspergillus oryzae bacterium number are 200,000,000 CFU/g, 400,000,000 CFU/g of lactobacillus plantarum.
Finished product can be obtained after banking up ageing after fermentation 20 days.
Embodiment 5:
A kind of bio-organic fertilizer suitable for rape, is obtained by following step:
550kg distillers ' grains powder, 150kg rape dregs of rice powder, 230kg rape straw, 67kg cray shell powder are mixed, adjustment
Moisture is to 55%, and inoculation 3kg complex micro organism fungicide is uniformly mixed, daily turning after fermentation temperature is increased to 60 DEG C or more, often
It 1-2 times, fermentation period 28 days, the overall weight of material was the 60% of fermentation initial stage weight in wet base.The environment temperature of the present embodiment is
5-20℃。
Complex micro organism fungicide used is the same as embodiment 4；
Finished product can be obtained after banking up ageing after fermentation 20 days.
Embodiment 6:
The physical and chemical index of organic fertilizer obtained:
Referring to the index and method of NY884-2012 biological organic fertilizer professional standard defined, to embodiment 4 and embodiment 5
Biological organic fertilizer obtained is detected, as a result as shown in table 3 below, the living bacteria count in two samples is respectively 12.7 hundred million/
G and 15.2 hundred million/g is much higher than 0.20 hundred million/g of professional standard.Other technologies index also all meets the requirement of NY884-2012.
3 biological organic fertilizer testing result of table
| Project | 4 sample of embodiment | 5 sample of embodiment | Technical indicator |
| Living bacteria count (CFU), hundred million/g | 12.7 | 15.2 | ≥0.20 |
| Organic matter (in terms of butt), % | 73.7 | 75.2 | ≥40.0 |
| Moisture, % | 25.6 | 22.8 | ≤30.0 |
| pH | 6.2 | 6.1 | 5.5-8.5 |
| Excrement colibacillus group number, a/g | 18 | 23 | ≤100 |
| Induced worm egg death rate, % | 100 | 100 | ≥95 |
| Total arsenic (As) (in terms of butt), mg/kg | 0.2 | 0.2 | ≤15 |
| Total cadmium (Cd) (in terms of butt), mg/kg | 0.3 | 0.4 | ≤3 |
| Total lead (Pb) (in terms of butt), mg/kg | It is not detected | It is not detected | ≤50 |
| Total chromium (Cr) (in terms of butt), mg/kg | 0.9 | 1.1 | ≤150 |
| Total mercury (Hg) (in terms of butt), mg/kg | It is not detected | It is not detected | ≤2 |
| Free aminoacid content (in terms of butt), % | 1.21 | 1.08 | / |
Aflatoxin and sclerotinite detection are carried out to biological organic fertilizer made from embodiment 4, as a result as shown in table 4 below,
Aflatoxin B in organic fertilizer1Substantially degraded, degradation rate 65%；And after During High-Temperature Composting is fermented, in organic fertilizer
Sclerotinite sporocyst all kill, can't detect after fermentation.The biological organic fertilizer can be used safely in cole crop fertilising.
Mycotoxin and sclerotinite testing result in 4 biological organic fertilizer of table
| Project name | Before fermentation | After fermentation |
| Aflatoxin B1(in terms of butt), μ g/kg | 10 | 3.5 |
| Nuclear disk bacterium number, a/kg | 120 | It is not detected |
Embodiment 7:
Biological organic fertilizer made from embodiment 4 is used for rapeseed cultivation test, to verify biological organic fertilizer to growth of rape
With the effect of disease control.
Test sets two processing altogether: (1) blank control, mu apply urea (H2NCONH2) 25kg, monoammonium phosphate (NH4H2PO4)
9kg, potassium chloride (KCl) 10kg, boron (Na2B4O7·10H2O)0.4kg；(2) biological organic fertilizer group, mu apply urea (H2NCONH2)
25kg, monoammonium phosphate (NH4H2PO4) 9kg, potassium chloride (KCl) 10kg, boron (Na2B4O7·10H2O) 0.4kg, biological organic fertilizer
50kg.Test is arranged by random district's groups, is repeated three times, each plot area 20m2.Testing rape variety is middle oil miscellaneous No. 5, oil
20000 plants/acre of colza density in planting, 600 plants of each cell, in Hua Zhong Agriculture University, experimental plot is completed in the school for test.
Test result is as shown in table 5, and biological organic fertilizer processing group is compared than blank control group, rape maturity period sclerotiniose hair
Sick rate reduces by 36.7%, and disease index declines 53.2%, output increased 12.2%.
Influence of 5 biological organic fertilizer of table to sclerotinia sclerotiorum and yield
| Project | Diseased plant rate (%) | Disease index | Yield |
| Control group | 9.8 | 8.25 | 2198.8 |
| Test group | 6.2 | 3.86 | 2467.0 |
| Amplification | - 36.7% | - 53.2% | + 12.2% |
In the present invention, lactobacillus plantarum L16 is to Sclerotinia sclerotiorum (sclerotinite Sclerotinia sclerotiorum)
There is good Inhibiting and protective function, the disease incidence of sclerotiniose can be effectively reduced.Aspergillus oryzae A08 has high protein enzyme activity simultaneously
Property, the crude protein in distillers ' grains and rapeseed meal can be decomposed well, improve the amino acid content in tunning.
Meanwhile microorganism can generate a large amount of heat during the fermentation, so as to make the water in fermentation substrate mixture
Divide and volatilize away, achievees the effect that dehydration, generated heat effectively can thoroughly kill harmful bacteria and worm's ovum.Condense gemma
Bacillus and lactobacillus plantarum L16 can generate lactic acid, and so that the chitin in cray shell is sloughed acetyl molecular radical, to become shell poly-
Sugar.Chitosan has inhibition to the spore germination of phytopathogen and mycelia growth, prevents pathogenic bacterial infection plant
Shield function has inducing action, and can promote plant growth.
Claims (8)
1. a kind of biological organic fertilizer suitable for rape is fermented by carrying out compost fermentation to material with complex micro organism fungicide
After bank up and be aged to obtain the final product；
Component in the material is in parts by weight, comprising: distillers ' grains powder 45-55, rape dregs of rice 20-25, rape straw 15-
25, complex micro organism fungicide 0.3-0.5, cray shell powder 5-8；
The living bacteria count for meeting microbial bacterial agent is hundred million CFU/g of 110-150, comprising: thermophilic fungus destroyed wire bacterium 9-12 hundred million
Hundred million CFU/ of CFU/g, bacillus subtilis 80-120 hundred million CFU/g, trichoderma harzianum 1-3 hundred million CFU/g, bacillus coagulans 15-30
Hundred million CFU/g of g, Paenibacillus polymyxa 1-3, hundred million CFU/g of aspergillus oryzae 1-3, hundred million CFU/g of lactobacillus plantarum 3-5；
The thermophilic fungus destroyed wire are as follows:Myceliophthora thermophila MT1810, deposit number are as follows: CCTCC NO:
M2018705；
The aspergillus oryzae are as follows: aspergillus oryzae (Aspergillus oryzae) A08, deposit number are as follows: CCTCC NO:M
2019505；
The lactobacillus plantarum are as follows: lactobacillus plantarum (Lactobacillus plantaLum) L16, deposit number are as follows:
CCTCC NO:M2019502.
2. organic fertilizer according to claim 1, the component in the material is in parts by weight, comprising: distillers ' grains powder
50, the rape dregs of rice 20, rape straw 24, complex micro organism fungicide 0.5, cray shell powder 5.5.
3. organic fertilizer according to claim 1, the component in the material is in parts by weight, comprising: distillers ' grains powder
55, the rape dregs of rice 15, rape straw 23, complex micro organism fungicide 0.3, cray shell powder 6.7.
4. organic fertilizer according to claim 1, daily turning after fermentation temperature is increased to 60 DEG C or more is 1-2 times daily.
5. organic fertilizer according to claim 1, the overall weight of material is the 50%-60% of fermentation initial stage weight in wet base, that is, is sent out
Ferment terminates.
6. organic fertilizer according to claim 1 banks up ageing 10-20 days.
7. biological organic fertilizer described in claim 1 is in the application being prepared into anti-sclerotinia sclerotiorum organic fertilizer.
8. biological organic fertilizer described in claim 1 is in the application being prepared into Rape organic fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910620698.XA CN110396028B (en) | 2019-07-10 | 2019-07-10 | Biological organic fertilizer suitable for rape and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910620698.XA CN110396028B (en) | 2019-07-10 | 2019-07-10 | Biological organic fertilizer suitable for rape and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN110396028A true CN110396028A (en) | 2019-11-01 |
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Cited By (5)
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| CN110937944A (en) * | 2019-12-23 | 2020-03-31 | 安徽省紫园生态农业科技有限公司 | Preparation method and use method of fertilizer based on Chinese rose planting |
| CN112299910A (en) * | 2020-11-06 | 2021-02-02 | 合肥官亭林海生态农业示范园有限公司 | Composting fermentation process of microbial organic vinasse |
| CN112400906A (en) * | 2021-01-04 | 2021-02-26 | 河南比赛尔农业科技有限公司 | Rape plant waste bacterium powder preparation for preventing and treating plant root diseases and preparation method and application thereof |
| CN113773126A (en) * | 2021-09-27 | 2021-12-10 | 湖北新保得生物科技有限公司 | Biological organic fertilizer for preventing and treating clubroot of Chinese cabbage and application thereof |
| CN118126846A (en) * | 2024-05-06 | 2024-06-04 | 云南省林业和草原科学院 | A culture method and application of endophytic fungus strain of Pseudocerma chinensis |
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| CN118126846A (en) * | 2024-05-06 | 2024-06-04 | 云南省林业和草原科学院 | A culture method and application of endophytic fungus strain of Pseudocerma chinensis |
| CN118126846B (en) * | 2024-05-06 | 2024-07-12 | 云南省林业和草原科学院 | Culture method and application of pseudogold Mao Nasheng fungus strain |
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