CN105001021A - Total-element compound microorganism organic fertilizer and preparing technology thereof - Google Patents
Total-element compound microorganism organic fertilizer and preparing technology thereof Download PDFInfo
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- CN105001021A CN105001021A CN201510468295.XA CN201510468295A CN105001021A CN 105001021 A CN105001021 A CN 105001021A CN 201510468295 A CN201510468295 A CN 201510468295A CN 105001021 A CN105001021 A CN 105001021A
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Abstract
Total-element compound microorganism organic fertilizer comprises, by weight, 40-60 parts of bacillus, 4-6 parts of actinomycetes and 30-50 parts of chemical fertilizer. The bacillus is composed of bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus, bacillus pumilus and bacillus megatherium. The chemical fertilizer contains oxygen, phosphorus and potassium. The invention further provides a preparing technology of the organic fertilizer. The preparing technology is simple, feasible, scientific, reasonable and suitable for industrial production. The prepared organic fertilizer integrates efficiency increasing of bio-fertilizer, long acting of organic fertilizer and quick acting of chemical fertilizer, the disease resistance, drought resistance and the low-temperature resistance of crops can be enhanced, the soil is loosened, and the hardening is removed; acid bases are conditioned, and the soil is repaired; residues are degraded, and the field is purified; the fertilizer efficiency is improved, and cost is reduced; the disaster is resisted, and stable yields despite drought or excessive rain are ensured; early maturing, quality upgrading and other metabolism mechanisms are promoted, the high yield of crops is increased, the quality of the crops is improved, and the economic income and social benefits of farmers are increased.
Description
Technical field
The present invention relates to microbial fertilizer technical field, particularly relate to a kind of complete first complex microorganism organic fertilizer material and preparation technology thereof.
Background technology
China consumes the chemical fertilizer of 5,900 ten thousand tons of nutrients resources at present every year, account for about 30% of world Fertilizer total burn-off, chemical nitrogen fertilizer utilization ratio only 30%, rate of loss is up to 60%, greatly consume the Nonrenewable resources (coal for chemical fertilizers production, Sweet natural gas), excessively for a long time use chemical fertilizer, China's Soil structure is caused to be seriously damaged, organic content sharply declines, a series of distinct issues such as structure of soil microbial community serious unbalance, therefore, Development of Novel fertilizer, strengthen soil vitality, with sustainable raising higher yield of crops, stable, seem abnormal important.
Tradition fertilizer available nutrient is low, and fertilizer efficiency is slow, and amount of application is large, and the man power and material of at substantial in manufacture and use procedure, economic benefit is low.Bio-feritlizer is movable by a large amount of beneficial microorganism, soil fertility can be increased, stimulate and regulation and control plant growth, strengthen degeneration-resistant and resistance against diseases, improve crop quality, but because itself is not containing nutritive element, it is a kind of indirect fertilizer, and be subject to the impact of natural temperature and soil fertility, to the generation of viable bacteria spore, survival has certain restricted influence, so chemical fertilizer and fertilizer can not be replaced completely, therefore, in the urgent need to developing a kind of novel complete first complex microorganism organic fertilizer material, reach and can improve output, Soil structure can be improved again, increase soil fertility, increase crop disease-resistant ability and improve soil organic matter content.
Summary of the invention
For the problems referred to above, the invention provides the synergy of a kind of collection biological bacteria, fertilizer is long-acting, complete first complex microorganism organic fertilizer material that chemical fertilizer is quick-acting to be integrated and preparation technology thereof.
For realizing above-mentioned purpose of the present invention, the invention provides a kind of complete first complex microorganism organic fertilizer material, being made up of the raw material of following parts by weight: genus bacillus 40-60 part, actinomycetes 4-6 part, chemical fertilizer 30-50 part.
Described genus bacillus is made up of subtilis, Bacillus licheniformis, Bacillus mucilaginosus, bacillus pumilus, bacillus megaterium; The weight ratio of described subtilis, Bacillus licheniformis, Bacillus mucilaginosus, bacillus pumilus, bacillus megaterium is 1:1:1:1:1.
Described chemical fertilizer is oxygen, phosphorus, potassium.
The present invention also provides a kind of preparation technology of complete first complex microorganism organic fertilizer material, comprise: adopt plate dilution method to carry out purifying agaric, rejuvenation is carried out after described purifying agaric, obtain one-level and produce bacterium, then Kolle flask or shaking table, obtain second class inoculum, microscopy after 48h, bacterial classification is pure, pollution-free miscellaneous bacteria, and namely accessible seeding tank ferments; Cultivation material put in advance by described seeding tank, 120 DEG C of sterilizing 30min, pH value 7.0-7.5, fermentation time 15-20h, it is many and proceed to fermentor tank without after miscellaneous bacteria that bacterium number observed by microscope mirror post, described fermentor tank needs to add load weighted fermentation materials before putting tank, stir material rear enclosed sterilizing 120 DEG C, sterilizing 30min, pH value 7.0-7.5, fermentation time 48-72h, microscopy is observed, the spore that fermented liquid produces is many and quantity reaches standard, with plate dilution method or microscope, the blue look dyeing of last leather, determines that bacterium number puts tank after errorless; Then be that sorbent material adsorbs with brown coal, carry out composite, stir after cryodrying, packaging, to obtain final product.
The weight ratio of described brown coal and bacterium is 7:3, and described brown coal reach 100-120 order through accurate pulverized particles density.
Compared with prior art beneficial effect of the present invention.
Complete first complex microorganism organic fertilizer material provided by the invention and preparation technology thereof, have the activating soil of biological bacteria concurrently, transform the function of soil nutrient, organic fertilizing soil, the improvement feature of soil and the quick-acting functions of inorganic nutrients, increase available nutrient in soil, oxygen, phosphorus, potassium and organic matter, add soil beneficial microorganism population and quantity, disease-resistant, the drought resisting of crop can be strengthened again simultaneously, cryophylactic ability and chessom, elimination harden; Conditioning soda acid, rehabilitating soil; Degrade residual, purification field; Improve fertilizer efficiency, reduce costs; Resist disaster, high yields irrespective of drought or water logging; Promote the metabolism such as precocity, upgrading quality; Preparation is simple for this complete first complex microorganism organic fertilizer material, scientific and reasonable, is suitable for suitability for industrialized production, to raising higher yield of crops, improves crop quality, and increasing peasant economy income has positive effect with social benefit.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
The invention provides a kind of complete first complex microorganism organic fertilizer material, be made up of the raw material of following parts by weight: genus bacillus 50 parts, 5 parts, actinomycetes, 45 parts, chemical fertilizer.
Described genus bacillus is made up of the raw material of following parts by weight: subtilis 10 parts, Bacillus licheniformis 10 parts, Bacillus mucilaginosus 10 parts, bacillus pumilus 10 parts, bacillus megaterium 10 parts.
Described chemical fertilizer is oxygen, phosphorus, potassium.
Described actinomycetes, subtilis, Bacillus licheniformis, Bacillus mucilaginosus, bacillus pumilus, bacillus megaterium are purchased from Agricultural University Of Shenyang, Institute of Microorganism, Academia Sinica.
The present invention also provides a kind of preparation technology of complete first complex microorganism organic fertilizer material, comprise: adopt plate dilution method to carry out purifying agaric, rejuvenation is carried out after described purifying agaric, obtain one-level and produce bacterium (inclined-plane), then shaking table, obtains second class inoculum, microscopy after 48h, bacterial classification is pure, pollution-free miscellaneous bacteria, and namely accessible seeding tank ferments, described seeding tank is put in advance and is cultivated material (peptone 0.05 part, yeast extract paste 0.5 part, potassium primary phosphate 0.2 part, 0.5 part, magnesium sulfate, iron trichloride 0.02 part), 120 DEG C of sterilizing 30min, pH value 7.0-7.5, fermentation time 15-20h, microscope mirror post is observed bacterium number many (more than 1/3) and is proceeded to fermentor tank without after miscellaneous bacteria, described fermentor tank needs to add load weighted fermentation materials (peptone 0.05 part before putting tank, yeast extract paste 0.5 part, potassium primary phosphate 0.2 part, 0.5 part, magnesium sulfate, iron trichloride 0.02 part), stir material rear enclosed, 120 DEG C of sterilizing 30min, pH value 7.0-7.5, fermentation time 48-72h, microscopy is observed, the spore that fermented liquid produces is many and quantity reaches standard (during observation, spore is full of observation window), the blue look dyeing of last leather, determine that bacterium number puts tank after errorless with plate dilution method or microscope, then be that sorbent material adsorbs with brown coal, cryodrying, then carries out composite, stirs, and packaging, to obtain final product.
The weight ratio of described brown coal and bacterium is 7:3, and described brown coal pick up from the maximum outdoor coal tomography in the whole nation of Tongliao, Inner Mongolia Huo Cun Guo Le, reach 100-120 order through accurate pulverized particles density.Employing brown coal carry out the best feature of absorption: after absorption, bacterium spore quantity is high, can reach 180-200 hundred million/g, and organic content is up to 60-70%; If dried through low temp dryer, bacterium spore quantity just can reach 300-400 hundred million/g, thus can ensure the quality that complete first complex microorganism organic fertilizer material is composite and effect again.
For determining the beneficial effect of the complete first complex microorganism organic fertilizer material of the present invention further, and for large-scale demonstration promote foundation is provided, the field demonstration of complete first complex microorganism organic fertilizer material result of use on grape has been carried out at Yao Qian street, Shujiatun District, Shenyang City major groove village Han Hai, specific as follows.
1, materials and methods.
1.1 demonstration place basic conditions: be located in the vineyard of Yao Qian street, Shujiatun District, Shenyang City major groove village Han Hai to demonstration, be experimental field the new yellow sand beach opened up wasteland, the average age of tree of grape is 5 years, and plantation level is suitable with local production level.
1.2 for examination fertilizer: complete first complex microorganism organic fertilizer material that the present embodiment provides, other fertilizer are prepared by Han Hai, and nitrogen, phosphorus, K composite fertilizer are purchased from the fraternal Fei Ye company limited in Shenyang four (nitrogen 15%, phosphorus 15%, potassium 15%).
1.3 for studying thing: grape, kind is huge rich grape.
1.4 test carrier and underwriters.
Test carrier: entrust Shujiatun District, Shenyang City agrotechnical station specifically to bear enforcement by Shenyang Xin Jiajie biotechnological formulation company limited.
Test underwriter: Li Jun (senior agronomist).
1.5 test process.
This test adopts simple contrast design, if two process, does not establish repetition, and each process is respectively.
Process 1, on the basis of peasant habit fertilising (fertilizer+nitrogen of chicken manure fermenting, phosphorus, K composite fertilizer), complete first complex microorganism organic fertilizer material 40kg that the present embodiment provides executed by every mu of base; In grape rudiment, bloom, Fruit and painted phase, complete execute with mu punching of pouring water first complex microorganism organic fertilizer material 20kg that the present embodiment provides respectively; Grape gather after base execute complete first complex microorganism organic fertilizer material 40kg that the present embodiment provides, use complete first complex microorganism organic fertilizer material 100kg that the present embodiment provides the time of infertility altogether, demonstration area area 20 mu.
Process 2, peasant habit fertilising (contrast), check plot area 20 mu.
Demonstration area and check plot fertilising kind, consumption, time, method require to carry out in strict accordance with exemplary design, and other field measures are completely the same.
2, demonstration result and analysis.
2.1 different treatment are on the impact of grape breeding time.
Table 1: each process grape record breeding time table (day/moon).
| Process | Budding period | Flowering period | Fruit expanding period | The fruit color phase | Ripening stage | Breeding time |
| 1 | 20/4 | 20/5 | 20/6 | 20/7 | 15/8 | 117 days |
| 2 | 22/4 | 24/5 | 27/6 | 30/7 | 27/8 | 127 days |
As shown in Table 1, process 1(demonstration area) breeding time comparatively process 2(check plot) shift to an earlier date to some extent, particularly, the budding period shifts to an earlier date 2 days, shifts to an earlier date 4 days flowering period, and fruit expanding period shifts to an earlier date 7 days, and the fruit color phase shifts to an earlier date 10 days, and the ripening stage shifts to an earlier date 12 days.Process 1(demonstration area) whole breeding time be 117 days, comparatively process 2(check plot) shorten 10 days.
2.2 different treatment are on the impact of Economic Characters of Grape: each process is chosen representational 5 grape seedlingses and observed and measure, and the every indexes of economic control value drawn, in table 2.
Table 2: the Economic Characters of Grape synopsis of different treatment.
| Process 1 | Process 2 | |
| Mu strain number (strain) | 330 | 330 |
| Take out tip number (group) | 14.5 | 13.8 |
| Fruit ear long (cm) | 26.0 | 26.7 |
| Single fruit transverse diameter (cm) | 2.02 | 1.94 |
| Single fruit spike number (cm) | 10.4 | 9.6 |
| Spike weight (g) | 1062.5 | 1011.0 |
| Single fruit weight (g) | 13.4 | 11.9 |
| Pol (%) | 14.98 | 10.63 |
As shown in Table 2, it is 14.5 groups that tip number is taken out in process 1, and comparatively processing 2 increases by 0.7 group; The fruit ear of process 1 is long is 26.0cm, comparatively processes 2 and reduces 0.7cm; The single fruit transverse diameter of process 1 is 2.02cm, and comparatively processing 2 increases 0.08cm; The single-strain tassel number of process 1 is 10.4 fringes, and comparatively processing 2 increases 1.5g; The pol of process 1 is 14.98%, and comparatively processing 2 increases by 4.35%.Therefore, the demonstration area process of complete first complex microorganism organic fertilizer of providing of the present embodiment effectively can increase the every indexes of economic of grape.
The impact that 2.3 different treatment produce grape.
Table 3: different treatment vintage contrast table.
| Process | Per mu yield (kg) | Mu volume increase (kg) | Stimulation ratio (%) |
| 1 | 3099.5 | 377.1 | 13.85 |
| 2 | 2722.4 | - | - |
As shown in Table 3, process 1(demonstration area) output be 3099.5kg, comparatively process 2(check plot) increase production 377.1kg, stimulation ratio is 13.85%.
2.4 economic efficiency contrast.
Table 4: different treatment economic efficiency contrast table.
| Process | Per mu yield (kg) | Per mu yield value (unit) | Mu drops into (unit) | Produce and throw ratio |
| 1 | 3099.5 | 29445.3 | 1438 | 20.48:1 |
| 2 | 2722.4 | 21779.2 | 1098 | 19.84:1 |
Note: process 1(demonstration area) grape unit price 9.5 yuan/kg, process 2(check plot) grape unit price 8 yuan/kg; Mu drops into and refers to every mu of fertilizers input.
As shown in Table 4, process 1(demonstration area) per mu yield value is 29445.3 yuan, comparatively processes 2(check plot) increase by 7666.1 yuan.Due to process 1(demonstration area) grape Time To Market in advance, average unit cost comparatively processes 2(check plot) increase by 1.5 yuan/kg, therefore, although process 1(demonstration area) add the input of complete first complex microorganism organic fertilizer, produce and throw than still higher than process 2(check plot).
3, conclusion.
The huge rich grape growing way using the complete first complex microorganism organic fertilizer of the present embodiment is vigorous, and disease and pest reduces, fruit uniform particles, colouring is fast, and fruit yield and quality are improved, and Time To Market shifts to an earlier date 10 days, add economic benefit, therefore, can use by spread on grape.
Claims (4)
1. a complete first complex microorganism organic fertilizer material, is characterized in that, be made up of following raw material: genus bacillus 40-60 part, actinomycetes 4-6 part, chemical fertilizer 30-50 part; Described genus bacillus is made up of subtilis, Bacillus licheniformis, Bacillus mucilaginosus, bacillus pumilus, bacillus megaterium; Described chemical fertilizer is oxygen, phosphorus, potassium.
2. complete first complex microorganism organic fertilizer material as claimed in claim 1, is characterized in that, the ratio of described subtilis, Bacillus licheniformis, Bacillus mucilaginosus, bacillus pumilus, bacillus megaterium is 1:1:1:1:1.
3. the preparation technology of complete first complex microorganism organic fertilizer material as claimed in claim 1, it is characterized in that, comprise: adopt plate dilution method to carry out purifying agaric, after described purifying agaric, carry out rejuvenation, obtain one-level and produce bacterium, then Kolle flask or shaking table, obtain second class inoculum, microscopy after 48h, bacterial classification is pure, pollution-free miscellaneous bacteria, namely accessible seeding tank ferments; Cultivation material put in advance by described seeding tank, 120 DEG C of sterilizing 30min, pH value 7.0-7.5, fermentation time 15-20h, it is many and proceed to fermentor tank without after miscellaneous bacteria that bacterium number observed by microscope mirror post, described fermentor tank needs to add load weighted fermentation materials before putting tank, stir material rear enclosed sterilizing 120 DEG C, sterilizing 30min, pH value 7.0-7.5, fermentation time 48-72h, microscopy is observed, the spore that fermented liquid produces is many and quantity reaches standard, with plate dilution method or microscope, the blue look dyeing of last leather, determines that bacterium number puts tank after errorless; Then be that sorbent material adsorbs with brown coal, carry out composite, stir after cryodrying, packaging, to obtain final product.
4. the preparation technology of complete first complex microorganism organic fertilizer material as claimed in claim 1, is characterized in that, the ratio of described brown coal and bacterium is 7:3, and described brown coal reach 100-120 order through accurate pulverized particles density.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
| CN106495904A (en) * | 2016-11-14 | 2017-03-15 | 四川农业大学 | A kind of preparation method and application of the special complete first biological organic fertilizer of cloud and mist 87 |
| CN110437010A (en) * | 2019-08-12 | 2019-11-12 | 沈阳市大光农业服务中心 | A kind of drug and two kinds of bacteria combination formula prevention and treatment crop dead seedlings and rotten technology |
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| CN1150133A (en) * | 1996-09-20 | 1997-05-21 | 北京大学 | Efficient biological compound fertilizer and its preparing process |
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| CN106008101A (en) * | 2016-05-25 | 2016-10-12 | 山西凯盛肥业集团有限公司 | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof |
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| CN110437010A (en) * | 2019-08-12 | 2019-11-12 | 沈阳市大光农业服务中心 | A kind of drug and two kinds of bacteria combination formula prevention and treatment crop dead seedlings and rotten technology |
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Application publication date: 20151028 |